Rroxscaffold_7G00203230

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0017411
Physical Location & Seq
Reverse (-)
51816923 .. 51823348
6426 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00203230.1

Sequence Viewer

Length: 846 bp
ATGGATCTGGTTTATATCATCCATTCTTTATCAATTGCTATATATAGGGACGATGTAGTTCCAATTAGATGGCCAGTGGAATATTCAAGCAGTTGCAGTGAATCTGATCCTCTGGAGTTCCTTTCAACACCATTATCTTGTCTCACTCTGAGAGACGAGCCAGCTGAAAATTCAACTGCATCTTATATGAGATGCGAAATACCTTTCGAAATGGGCTATATTGTGGAGAATTGCAAGACCTACTTGAGCGCTATTTCTTCCGTTAAGCTCACCTACACCGGCGGCGAGACCAAGATTCTCACCATCGAACGCACCATCAAATTCCCCGCCCTCGTCGCCGTGCTCTCCGCCCTCTCCGACAACGACGTGACGTTCAAGTACCAGCTCCCCGAGGAAGGCCTCAACGCCTTGATCTCCGTCACCAACGACGACGACCTTGAGCACGTGATGCACGAGTATGATCGCCTCTACCGGGCCTCCACCAGGATGAGGCTGTTTCTCTTCCCGGTCAACCACAGCGACAGTTTCGGGTTTGATAGGTCGTCCGATAGGGATCGCTTCGTTGTGGCTTTGAATTCGGGTCCCGAGATGCCGGATCCGGTGGCCCAAAATCCAGAAATTCAAACAAACTCGAAATGGCCAACCCCCAAATCTTCTCTCTACACCCAAAACCTCTTCTTTATGGGCACATCCAAATCACAGCCTCCCAAAACAACAGATTTCCCAACAGATAACAAAACCAACAGAGCACGTCTCCGTGATTGTTTACCCGAGGATGGACCGCAGATCCGCGACGGCAATCGGAGGTCGTGGGTACCCCACTGCAGGCATAACCACCGCCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

281

Amino Acids

32.13

Weight (kDa)

5.55

Isoelectric Point (pI)

49.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 87 - 169 4.9e-16 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 814
AccB1I GGYRCC 1 cut(s) 814
AccII CGCG 1 cut(s) 792
AciI CCGC 7 cut(s) 282, 327, 348, 782, 790, 838, 841
AclWI GGATC 6 cut(s) 12, 101, 561, 590, 603, 781
AcoI YGGCCR 2 cut(s) 71, 638
AcsI RAATTY 4 cut(s) 169, 320, 574, 618
AcvI CACGTG 1 cut(s) 445
AfaI GTAC 2 cut(s) 380, 816
AfeI AGCGCT 1 cut(s) 250
AfiI CCNNNNNNNGG 6 cut(s) 395, 472, 483, 489, 776, 825
AgsI TTSAA 6 cut(s) 87, 126, 174, 376, 574, 623
AjiI CACGTC 2 cut(s) 367, 752
AjnI CCWGG 1 cut(s) 482
AluBI AGCT 3 cut(s) 164, 268, 385
AluI AGCT 3 cut(s) 164, 268, 385
Alw21I GWGCWC 3 cut(s) 345, 444, 751
Alw26I GTCTC 4 cut(s) 146, 147, 281, 758
AlwI GGATC 6 cut(s) 12, 101, 561, 590, 603, 781
Ama87I CYCGRG 3 cut(s) 389, 584, 770
Aor51HI AGCGCT 1 cut(s) 250
AoxI GGCC 5 cut(s) 71, 397, 474, 603, 638
ApoI RAATTY 4 cut(s) 169, 320, 574, 618
Asp718I GGTACC 1 cut(s) 814
AspLEI GCGC 1 cut(s) 251
AspS9I GGNCC 4 cut(s) 474, 581, 604, 779
AsuC2I CCSGG 2 cut(s) 473, 506
AsuHPI GGTGA 3 cut(s) 262, 292, 412
AsuII TTCGAA 1 cut(s) 207
AvaI CYCGRG 3 cut(s) 389, 584, 770
AvaII GGWCC 2 cut(s) 581, 779
BaeGI GKGCMC 1 cut(s) 689
BalI TGGCCA 2 cut(s) 73, 640
BamHI GGATCC 1 cut(s) 595
BanI GGYRCC 1 cut(s) 814
BauI CACGAG 1 cut(s) 452
BbrPI CACGTG 1 cut(s) 445
Bbv12I GWGCWC 3 cut(s) 345, 444, 751
BccI CCATC 4 cut(s) 63, 311, 323, 770
BceAI ACGGC 2 cut(s) 323, 811
BciT130I CCWGG 1 cut(s) 484
BcnI CCSGG 2 cut(s) 473, 506
BcoDI GTCTC 4 cut(s) 146, 147, 281, 758
BfaI CTAG 1 cut(s) 844
BfmI CTRYAG 1 cut(s) 823
BfoI RGCGCY 1 cut(s) 252
BisI GCNGC 2 cut(s) 283, 841
BlsI GCNGC 2 cut(s) 284, 842
Bme1390I CCNGG 3 cut(s) 473, 484, 506
Bme18I GGWCC 2 cut(s) 581, 779
BmeT110I CYCGRG 3 cut(s) 389, 584, 770
BmgBI CACGTC 2 cut(s) 367, 752
BmgT120I GGNCC 4 cut(s) 474, 581, 604, 779
BmiI GGNNCC 4 cut(s) 582, 583, 597, 816
BmrFI CCNGG 3 cut(s) 473, 484, 506
BmsI GCATC 4 cut(s) 182, 188, 438, 579
BplI GAGNNNNNCTC 2 cut(s) 738, 770
BpmI CTGGAG 1 cut(s) 134
Bpu14I TTCGAA 1 cut(s) 207
BpuEI CTTGAG 2 cut(s) 265, 458
BpuMI CCSGG 2 cut(s) 473, 506
BsaAI YACGTR 1 cut(s) 445
BsaBI GATNNNNATC 1 cut(s) 552
BsaI GGTCTC 1 cut(s) 281
BsaJI CCNNGG 2 cut(s) 390, 771
BsaWI WCCGGW 1 cut(s) 598
BsaXI ACNNNNNCTCC 2 cut(s) 461, 491
Bsc4I CCNNNNNNNGG 6 cut(s) 395, 472, 483, 489, 776, 825
Bse118I RCCGGY 1 cut(s) 278
Bse1I ACTGG 1 cut(s) 74
Bse8I GATNNNNATC 1 cut(s) 552
BseBI CCWGG 1 cut(s) 484
BseDI CCNNGG 2 cut(s) 390, 771
BseGI GGATG 4 cut(s) 18, 492, 689, 781
BseJI GATNNNNATC 1 cut(s) 552
BseLI CCNNNNNNNGG 6 cut(s) 395, 472, 483, 489, 776, 825
BseMII CTCAG 1 cut(s) 140
BseNI ACTGG 1 cut(s) 74
BseSI GKGCMC 1 cut(s) 689
Bsh1236I CGCG 1 cut(s) 792
BshFI GGCC 5 cut(s) 73, 399, 476, 605, 640
BshNI GGYRCC 1 cut(s) 814
BsiHKAI GWGCWC 3 cut(s) 345, 444, 751
BsiHKCI CYCGRG 3 cut(s) 389, 584, 770
BsiSI CCGG 5 cut(s) 279, 472, 506, 593, 599
BslFI GGGAC 2 cut(s) 62, 567
BslI CCNNNNNNNGG 6 cut(s) 395, 472, 483, 489, 776, 825
BsmAI GTCTC 4 cut(s) 146, 147, 281, 758
BsmBI CGTCTC 2 cut(s) 147, 758
BsmFI GGGAC 2 cut(s) 62, 567
BsnI GGCC 5 cut(s) 73, 399, 476, 605, 640
Bso31I GGTCTC 1 cut(s) 281
BsoBI CYCGRG 3 cut(s) 389, 584, 770
Bsp119I TTCGAA 1 cut(s) 207
Bsp1286I GDGCHC 4 cut(s) 345, 444, 689, 751
Bsp143I GATC 7 cut(s) 4, 106, 411, 460, 553, 595, 786
BspACI CCGC 7 cut(s) 282, 327, 348, 782, 790, 838, 841
BspANI GGCC 5 cut(s) 73, 399, 476, 605, 640
BspCNI CTCAG 1 cut(s) 141
BspFNI CGCG 1 cut(s) 792
BspLI GGNNCC 4 cut(s) 582, 583, 597, 816
BspMAI CTGCAG 1 cut(s) 827
BspPI GGATC 6 cut(s) 12, 101, 561, 590, 603, 781
BspT104I TTCGAA 1 cut(s) 207
BspT107I GGYRCC 1 cut(s) 814
BspTNI GGTCTC 1 cut(s) 281
BsrFI RCCGGY 1 cut(s) 278
BsrI ACTGG 1 cut(s) 74
BssAI RCCGGY 1 cut(s) 278
BssECI CCNNGG 2 cut(s) 390, 771
BssMI GATC 7 cut(s) 4, 106, 411, 460, 553, 595, 786
BssSI CACGAG 1 cut(s) 452
Bst2BI CACGAG 1 cut(s) 452
Bst2UI CCWGG 1 cut(s) 484
Bst4CI ACNGT 1 cut(s) 524
Bst6I CTCTTC 2 cut(s) 506, 680
BstAPI GCANNNNNTGC 1 cut(s) 448
BstBAI YACGTR 1 cut(s) 445
BstBI TTCGAA 1 cut(s) 207
BstC8I GCNNGC 2 cut(s) 162, 827
BstDEI CTNAG 1 cut(s) 149
BstENI CCTNNNNNAGG 1 cut(s) 481
BstF5I GGATG 4 cut(s) 18, 492, 689, 781
BstFNI CGCG 1 cut(s) 792
BstH2I RGCGCY 1 cut(s) 252
BstHHI GCGC 1 cut(s) 251
BstKTI GATC 7 cut(s) 7, 109, 414, 463, 556, 598, 789
BstMAI GTCTC 4 cut(s) 146, 147, 281, 758
BstMBI GATC 7 cut(s) 4, 106, 411, 460, 553, 595, 786
BstMWI GCNNNNNNNGC 2 cut(s) 335, 448
BstNI CCWGG 1 cut(s) 484
BstSCI CCNGG 3 cut(s) 471, 482, 504
BstSFI CTRYAG 1 cut(s) 823
BstSLI GKGCMC 1 cut(s) 689
BstUI CGCG 1 cut(s) 792
BstX2I RGATCY 3 cut(s) 4, 595, 786
BstXI CCANNNNNNTGG 1 cut(s) 69
BstYI RGATCY 3 cut(s) 4, 595, 786
BsuRI GGCC 5 cut(s) 73, 399, 476, 605, 640
BtrI CACGTC 2 cut(s) 367, 752
BtsCI GGATG 4 cut(s) 18, 492, 689, 781
BtsI GCAGTG 2 cut(s) 103, 820
BtsIMutI CAGTG 3 cut(s) 81, 103, 820
Cac8I GCNNGC 2 cut(s) 162, 827
CfoI GCGC 1 cut(s) 251
Cfr10I RCCGGY 1 cut(s) 278
Cfr13I GGNCC 4 cut(s) 474, 581, 604, 779
Csp6I GTAC 2 cut(s) 379, 815
CviQI GTAC 2 cut(s) 379, 815
DdeI CTNAG 1 cut(s) 149
DpnI GATC 7 cut(s) 6, 108, 413, 462, 555, 597, 788
DpnII GATC 7 cut(s) 4, 106, 411, 460, 553, 595, 786
EaeI YGGCCR 2 cut(s) 71, 638
Eam1104I CTCTTC 2 cut(s) 506, 680
EarI CTCTTC 2 cut(s) 506, 680
EciI GGCGGA 1 cut(s) 337
Eco147I AGGCCT 1 cut(s) 399
Eco31I GGTCTC 1 cut(s) 281
Eco47I GGWCC 2 cut(s) 581, 779
Eco47III AGCGCT 1 cut(s) 250
Eco72I CACGTG 1 cut(s) 445
Eco88I CYCGRG 3 cut(s) 389, 584, 770
EcoNI CCTNNNNNAGG 1 cut(s) 481
EcoO109I RGGNCCY 1 cut(s) 581
EcoRI GAATTC 1 cut(s) 574
EcoRII CCWGG 1 cut(s) 482
Esp3I CGTCTC 2 cut(s) 147, 758
FalI AAGNNNNNCTT 2 cut(s) 227, 259
FaqI GGGAC 2 cut(s) 62, 567
FauI CCCGC 1 cut(s) 334
Fnu4HI GCNGC 2 cut(s) 283, 841
FokI GGATG 4 cut(s) 5, 499, 676, 788
Fsp4HI GCNGC 2 cut(s) 283, 841
FspBI CTAG 1 cut(s) 844
GlaI GCGC 1 cut(s) 250
GluI GCNGC 2 cut(s) 283, 841
GsuI CTGGAG 1 cut(s) 134
HaeII RGCGCY 1 cut(s) 252
HaeIII GGCC 5 cut(s) 73, 399, 476, 605, 640
HapII CCGG 5 cut(s) 279, 472, 506, 593, 599
HhaI GCGC 1 cut(s) 251
Hin6I GCGC 1 cut(s) 249
HinP1I GCGC 1 cut(s) 249
HincII GTYRAC 1 cut(s) 511
HindII GTYRAC 1 cut(s) 511
HinfI GANTC 2 cut(s) 101, 295
HpaII CCGG 5 cut(s) 279, 472, 506, 593, 599
HphI GGTGA 3 cut(s) 262, 292, 412
Hpy166II GTNNAC 2 cut(s) 511, 767
Hpy188I TCNGA 5 cut(s) 106, 150, 358, 547, 804
Hpy188III TCNNGA 3 cut(s) 113, 584, 614
Hpy8I GTNNAC 2 cut(s) 511, 767
Hpy99I CGWCG 5 cut(s) 338, 368, 431, 434, 797
HpyAV CCTTC 1 cut(s) 389
HpyCH4III ACNGT 1 cut(s) 524
HpyCH4IV ACGT 4 cut(s) 366, 371, 444, 751
HpyCH4V TGCA 5 cut(s) 96, 179, 234, 451, 825
HpyF10VI GCNNNNNNNGC 2 cut(s) 335, 448
HpyF3I CTNAG 1 cut(s) 149
HpySE526I ACGT 4 cut(s) 366, 371, 444, 751
HspAI GCGC 1 cut(s) 249
KflI GGGWCCC 1 cut(s) 581
KpnI GGTACC 1 cut(s) 818
Kzo9I GATC 7 cut(s) 4, 106, 411, 460, 553, 595, 786
LmnI GCTCC 1 cut(s) 390
LweI GCATC 4 cut(s) 182, 188, 438, 579
MaeI CTAG 1 cut(s) 844
MaeII ACGT 4 cut(s) 366, 371, 444, 751
MaeIII GTNAC 2 cut(s) 367, 418
MalI GATC 7 cut(s) 6, 108, 413, 462, 555, 597, 788
MboI GATC 7 cut(s) 4, 106, 411, 460, 553, 595, 786
MboII GAAGA 4 cut(s) 249, 493, 645, 667
MfeI CAATTG 1 cut(s) 33
MflI RGATCY 3 cut(s) 4, 595, 786
MhlI GDGCHC 4 cut(s) 345, 444, 689, 751
MlsI TGGCCA 2 cut(s) 73, 640
MluCI AATT 7 cut(s) 33, 63, 169, 229, 320, 574, 618
MluNI TGGCCA 2 cut(s) 73, 640
MmeI TCCRAC 1 cut(s) 381
Mox20I TGGCCA 2 cut(s) 73, 640
MscI TGGCCA 2 cut(s) 73, 640
MseI TTAA 1 cut(s) 264
MslI CAYNNNNRTG 2 cut(s) 456, 485
Msp20I TGGCCA 2 cut(s) 73, 640
MspA1I CMGCKG 1 cut(s) 164
MspI CCGG 5 cut(s) 279, 472, 506, 593, 599
MspR9I CCNGG 3 cut(s) 473, 484, 506
MunI CAATTG 1 cut(s) 33
MvaI CCWGG 1 cut(s) 484
MvnI CGCG 1 cut(s) 792
MwoI GCNNNNNNNGC 2 cut(s) 335, 448
NciI CCSGG 2 cut(s) 473, 506
NdeII GATC 7 cut(s) 4, 106, 411, 460, 553, 595, 786
NlaIV GGNNCC 4 cut(s) 582, 583, 597, 816
NmuCI GTSAC 2 cut(s) 367, 418
NspV TTCGAA 1 cut(s) 207
PceI AGGCCT 1 cut(s) 399
PcsI WCGNNNNNNNCGW 1 cut(s) 450
PfeI GAWTC 2 cut(s) 101, 295
PkrI GCNGC 2 cut(s) 284, 842
PmaCI CACGTG 1 cut(s) 445
PmlI CACGTG 1 cut(s) 445
Ppu21I YACGTR 1 cut(s) 445
PpuMI RGGWCCY 1 cut(s) 581
Psp5II RGGWCCY 1 cut(s) 581
Psp6I CCWGG 1 cut(s) 482
PspCI CACGTG 1 cut(s) 445
PspGI CCWGG 1 cut(s) 482
PspN4I GGNNCC 4 cut(s) 582, 583, 597, 816
PspPI GGNCC 4 cut(s) 474, 581, 604, 779
PspPPI RGGWCCY 1 cut(s) 581
PstI CTGCAG 1 cut(s) 827
PsuI RGATCY 3 cut(s) 4, 595, 786
PvuII CAGCTG 1 cut(s) 164
RsaI GTAC 2 cut(s) 380, 816
RsaNI GTAC 2 cut(s) 379, 815
RseI CAYNNNNRTG 2 cut(s) 456, 485
SaqAI TTAA 1 cut(s) 264
SatI GCNGC 2 cut(s) 283, 841
Sau3AI GATC 7 cut(s) 4, 106, 411, 460, 553, 595, 786
Sau96I GGNCC 4 cut(s) 474, 581, 604, 779
ScrFI CCNGG 3 cut(s) 473, 484, 506
SduI GDGCHC 4 cut(s) 345, 444, 689, 751
SfaNI GCATC 4 cut(s) 182, 188, 438, 579
SfcI CTRYAG 1 cut(s) 823
SfuI TTCGAA 1 cut(s) 207
SgrAI CRCCGGYG 1 cut(s) 278
SinI GGWCC 2 cut(s) 581, 779
SmiMI CAYNNNNRTG 2 cut(s) 456, 485
SmlI CTYRAG 2 cut(s) 244, 437
SmoI CTYRAG 2 cut(s) 244, 437
Sse9I AATT 7 cut(s) 33, 63, 169, 229, 320, 574, 618
SseBI AGGCCT 1 cut(s) 399
SsiI CCGC 7 cut(s) 282, 327, 348, 782, 790, 838, 841
SspI AATATT 1 cut(s) 83
SspMI CTAG 1 cut(s) 844
StuI AGGCCT 1 cut(s) 399
StyD4I CCNGG 3 cut(s) 471, 482, 504
TaaI ACNGT 1 cut(s) 524
TaiI ACGT 4 cut(s) 369, 374, 447, 754
TaqI TCGA 3 cut(s) 207, 306, 632
TasI AATT 7 cut(s) 33, 63, 169, 229, 320, 574, 618
TauI GCSGC 2 cut(s) 285, 843
TfiI GAWTC 2 cut(s) 101, 295
Tru1I TTAA 1 cut(s) 264
Tru9I TTAA 1 cut(s) 264
TscAI CASTG 3 cut(s) 81, 103, 827
TseFI GTSAC 2 cut(s) 367, 418
Tsp45I GTSAC 2 cut(s) 367, 418
TspGWI ACGGA 3 cut(s) 250, 406, 746
TspRI CASTG 3 cut(s) 81, 103, 827
VpaK11BI GGWCC 2 cut(s) 581, 779
XagI CCTNNNNNAGG 1 cut(s) 481
XapI RAATTY 4 cut(s) 169, 320, 574, 618
XspI CTAG 1 cut(s) 844
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.