Rmu_sc0005995.1_g000010

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005995.1
OG0011771
Physical Location & Seq
Forward (+)
48040 .. 49382
1343 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 654 bp
atgggttccgcagatgaagatgggcctgaaacaggtaatgtaccacaaacaggtggtgctggggaattcgatccggctcaaattgttgccaaagccgtgctgtgtttcaataacaactatgtatacagcagctgcgaacagtcttttagattgaatgagagtggagatctgaatgttccaaaagacaaaaccgacgagttctgcaatggtgcttgcctcacagagacacaccttgtgctcaactgtgttgatcacattttggccaacttcgtcttctacaacaaggccactataagagatgttagagatactgttcaggccggatgtggatctggccctgaaagaggcaacttcaacgtggaggagcatatggctgagacgagcaaggcacgcaagcctgcaacttaccagattcttgttgggcttgttttgatgagaggatcagaagcggacgttcgcaatgccgtaaaagtgcggacgtcttctgacgcgattaggcgcggcactattcttgcggaacaaagaccagacgtcgatcttgagcttcttcttgccggtggactcgccgcctaccagtttgcagtccaccttgatcggggtgcccatggtctccgtttggcaagagtcgcgccgccttcggcgcctgatcgttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Endosulfine

Protein Analysis

217

Amino Acids

23.41

Weight (kDa)

5.25

Isoelectric Point (pI)

32.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7731 PF24865 25 - 124 3.8e-44 Domain of unknown function (DUF7731)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011771)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49350 AT5G49350
fragaria_vesca FvH4_4g19330
malus_domestica MD13G1157600.v1.1 MD16G1158500.v1.1
prunus_persica Prupe.1G123300_v2.0.a1
pyrus_communis pycom13g13490 pycom16g13340
rosa_chinensis RchiOBHm_Chr4g0424351
rosa_laevigata RLG00000007482
rosa_multiflora Rmu_sc0005995.1_g000010
rosa_roxburghii Rroxscaffold_5G00366300
rosa_rugosa Rorug04G0196600 Rorug04G0196700
rosa_samantha Rh4AG253000 Rh4BG258400 Rh4CG269400 Rh4DG252800
rosa_wichuraiana Rw4G021820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 482, 534
AccB1I GGYRCC 2 cut(s) 599, 640
AccI GTMKAC 1 cut(s) 123
AccII CGCG 3 cut(s) 491, 501, 629
AciI CCGC 7 cut(s) 9, 449, 475, 501, 515, 567, 632
AclWI GGATC 3 cut(s) 65, 337, 448
AcoI YGGCCR 1 cut(s) 261
AcsI RAATTY 1 cut(s) 65
AcyI GRCGYC 3 cut(s) 479, 531, 641
AdeI CACNNNGTG 1 cut(s) 235
AfaI GTAC 1 cut(s) 42
AfiI CCNNNNNNNGG 4 cut(s) 32, 50, 344, 595
AgsI TTSAA 3 cut(s) 109, 154, 355
AhdI GACNNNNNGTC 1 cut(s) 530
AjuI GAANNNNNNNTTGG 2 cut(s) 257, 289
AluBI AGCT 2 cut(s) 132, 544
AluI AGCT 2 cut(s) 132, 544
Alw21I GWGCWC 1 cut(s) 240
Alw26I GTCTC 3 cut(s) 218, 371, 614
AlwI GGATC 3 cut(s) 65, 337, 448
AlwNI CAGNNNCTG 1 cut(s) 132
AoxI GGCC 5 cut(s) 23, 261, 285, 318, 334
ApeKI GCWGC 2 cut(s) 129, 132
ApoI RAATTY 1 cut(s) 65
AspLEI GCGC 3 cut(s) 501, 631, 643
AspS9I GGNCC 2 cut(s) 23, 335
BaeGI GKGCMC 1 cut(s) 604
BalI TGGCCA 1 cut(s) 263
BanI GGYRCC 2 cut(s) 599, 640
BbsI GAAGAC 2 cut(s) 265, 474
Bbv12I GWGCWC 1 cut(s) 240
BbvI GCAGC 2 cut(s) 119, 141
BccI CCATC 1 cut(s) 14
BceAI ACGGC 2 cut(s) 80, 449
BclI TGATCA 1 cut(s) 250
BcoDI GTCTC 3 cut(s) 218, 371, 614
BfoI RGCGCY 1 cut(s) 644
BglII AGATCT 1 cut(s) 166
BisI GCNGC 5 cut(s) 130, 133, 502, 567, 632
BlsI GCNGC 5 cut(s) 131, 134, 503, 568, 633
BmeRI GACNNNNNGTC 1 cut(s) 530
BmgT120I GGNCC 2 cut(s) 23, 335
BmiI GGNNCC 3 cut(s) 7, 601, 642
BpiI GAAGAC 2 cut(s) 265, 474
BpuEI CTTGAG 1 cut(s) 560
BsaBI GATNNNNATC 1 cut(s) 328
BsaHI GRCGYC 3 cut(s) 479, 531, 641
BsaI GGTCTC 1 cut(s) 614
BsaJI CCNNGG 1 cut(s) 604
Bsc4I CCNNNNNNNGG 4 cut(s) 32, 50, 344, 595
Bse118I RCCGGY 1 cut(s) 554
Bse1I ACTGG 1 cut(s) 574
Bse3DI GCAATG 2 cut(s) 211, 466
Bse8I GATNNNNATC 1 cut(s) 328
BseDI CCNNGG 1 cut(s) 604
BseGI GGATG 1 cut(s) 329
BseJI GATNNNNATC 1 cut(s) 328
BseLI CCNNNNNNNGG 4 cut(s) 32, 50, 344, 595
BseMI GCAATG 2 cut(s) 211, 466
BseMII CTCAG 1 cut(s) 366
BseNI ACTGG 1 cut(s) 574
BseRI GAGGAG 1 cut(s) 377
BseSI GKGCMC 1 cut(s) 604
BseXI GCAGC 2 cut(s) 119, 141
BseYI CCCAGC 1 cut(s) 59
Bsh1236I CGCG 3 cut(s) 491, 501, 629
BshFI GGCC 5 cut(s) 25, 263, 287, 320, 336
BshNI GGYRCC 2 cut(s) 599, 640
BsiHKAI GWGCWC 1 cut(s) 240
BsiSI CCGG 3 cut(s) 74, 321, 555
BslI CCNNNNNNNGG 4 cut(s) 32, 50, 344, 595
BsmAI GTCTC 3 cut(s) 218, 371, 614
BsmBI CGTCTC 1 cut(s) 371
BsnI GGCC 5 cut(s) 25, 263, 287, 320, 336
Bso31I GGTCTC 1 cut(s) 614
Bsp1286I GDGCHC 2 cut(s) 240, 604
Bsp143I GATC 8 cut(s) 70, 166, 250, 329, 440, 535, 592, 646
Bsp19I CCATGG 1 cut(s) 604
BspACI CCGC 7 cut(s) 9, 449, 475, 501, 515, 567, 632
BspANI GGCC 5 cut(s) 25, 263, 287, 320, 336
BspCNI CTCAG 1 cut(s) 367
BspFNI CGCG 3 cut(s) 491, 501, 629
BspLI GGNNCC 3 cut(s) 7, 601, 642
BspPI GGATC 3 cut(s) 65, 337, 448
BspT107I GGYRCC 2 cut(s) 599, 640
BspTNI GGTCTC 1 cut(s) 614
BsrDI GCAATG 2 cut(s) 211, 466
BsrFI RCCGGY 1 cut(s) 554
BsrI ACTGG 1 cut(s) 574
BssAI RCCGGY 1 cut(s) 554
BssECI CCNNGG 1 cut(s) 604
BssMI GATC 8 cut(s) 70, 166, 250, 329, 440, 535, 592, 646
BssNAI GTATAC 1 cut(s) 124
BssNI GRCGYC 3 cut(s) 479, 531, 641
BssT1I CCWWGG 1 cut(s) 604
Bst1107I GTATAC 1 cut(s) 124
Bst4CI ACNGT 3 cut(s) 141, 245, 313
BstACI GRCGYC 3 cut(s) 479, 531, 641
BstC8I GCNNGC 4 cut(s) 214, 391, 395, 399
BstDEI CTNAG 1 cut(s) 375
BstDSI CCRYGG 1 cut(s) 604
BstENI CCTNNNNNAGG 2 cut(s) 30, 342
BstF5I GGATG 1 cut(s) 329
BstFNI CGCG 3 cut(s) 491, 501, 629
BstH2I RGCGCY 1 cut(s) 644
BstHHI GCGC 3 cut(s) 501, 631, 643
BstKTI GATC 8 cut(s) 73, 169, 253, 332, 443, 538, 595, 649
BstMAI GTCTC 3 cut(s) 218, 371, 614
BstMBI GATC 8 cut(s) 70, 166, 250, 329, 440, 535, 592, 646
BstMWI GCNNNNNNNGC 3 cut(s) 390, 626, 640
BstSLI GKGCMC 1 cut(s) 604
BstUI CGCG 3 cut(s) 491, 501, 629
BstV1I GCAGC 2 cut(s) 119, 141
BstV2I GAAGAC 2 cut(s) 265, 474
BstX2I RGATCY 2 cut(s) 166, 329
BstYI RGATCY 2 cut(s) 166, 329
BstZ17I GTATAC 1 cut(s) 124
BsuRI GGCC 5 cut(s) 25, 263, 287, 320, 336
BtgI CCRYGG 1 cut(s) 604
BtsCI GGATG 1 cut(s) 329
Cac8I GCNNGC 4 cut(s) 214, 391, 395, 399
CaiI CAGNNNCTG 1 cut(s) 132
CfoI GCGC 3 cut(s) 501, 631, 643
Cfr10I RCCGGY 1 cut(s) 554
Cfr13I GGNCC 2 cut(s) 23, 335
CseI GACGC 1 cut(s) 497
Csp6I GTAC 1 cut(s) 41
CviAII CATG 1 cut(s) 605
CviQI GTAC 1 cut(s) 41
DdeI CTNAG 1 cut(s) 375
DinI GGCGCC 1 cut(s) 642
DpnI GATC 8 cut(s) 72, 168, 252, 331, 442, 537, 594, 648
DpnII GATC 8 cut(s) 70, 166, 250, 329, 440, 535, 592, 646
DraIII CACNNNGTG 1 cut(s) 235
DriI GACNNNNNGTC 1 cut(s) 530
EaeI YGGCCR 1 cut(s) 261
Eam1105I GACNNNNNGTC 1 cut(s) 530
Eco130I CCWWGG 1 cut(s) 604
Eco31I GGTCTC 1 cut(s) 614
EcoNI CCTNNNNNAGG 2 cut(s) 30, 342
EcoRI GAATTC 1 cut(s) 65
EcoT14I CCWWGG 1 cut(s) 604
EgeI GGCGCC 1 cut(s) 642
EheI GGCGCC 1 cut(s) 642
ErhI CCWWGG 1 cut(s) 604
Esp3I CGTCTC 1 cut(s) 371
FaeI CATG 1 cut(s) 608
FaiI YATR 6 cut(s) 120, 124, 293, 369, 371, 606
FatI CATG 1 cut(s) 604
FauNDI CATATG 1 cut(s) 369
FbaI TGATCA 1 cut(s) 250
FblI GTMKAC 1 cut(s) 123
Fnu4HI GCNGC 5 cut(s) 130, 133, 502, 567, 632
FokI GGATG 1 cut(s) 336
Fsp4HI GCNGC 5 cut(s) 130, 133, 502, 567, 632
GlaI GCGC 3 cut(s) 500, 630, 642
GluI GCNGC 5 cut(s) 130, 133, 502, 567, 632
GsaI CCCAGC 1 cut(s) 63
HaeII RGCGCY 1 cut(s) 644
HaeIII GGCC 5 cut(s) 25, 263, 287, 320, 336
HapII CCGG 3 cut(s) 74, 321, 555
HgaI GACGC 1 cut(s) 497
HhaI GCGC 3 cut(s) 501, 631, 643
Hin1I GRCGYC 3 cut(s) 479, 531, 641
Hin1II CATG 1 cut(s) 608
Hin6I GCGC 3 cut(s) 499, 629, 641
HinP1I GCGC 3 cut(s) 499, 629, 641
HinfI GANTC 3 cut(s) 412, 561, 624
HpaII CCGG 3 cut(s) 74, 321, 555
Hpy166II GTNNAC 3 cut(s) 124, 560, 586
Hpy188I TCNGA 3 cut(s) 171, 445, 487
Hpy188III TCNNGA 1 cut(s) 539
Hpy8I GTNNAC 3 cut(s) 124, 560, 586
Hpy99I CGWCG 2 cut(s) 197, 536
HpyAV CCTTC 1 cut(s) 645
HpyCH4III ACNGT 3 cut(s) 141, 245, 313
HpyCH4IV ACGT 4 cut(s) 357, 453, 479, 531
HpyCH4V TGCA 3 cut(s) 204, 401, 581
HpyF10VI GCNNNNNNNGC 3 cut(s) 390, 626, 640
HpyF3I CTNAG 1 cut(s) 375
HpySE526I ACGT 4 cut(s) 357, 453, 479, 531
Hsp92I GRCGYC 3 cut(s) 479, 531, 641
Hsp92II CATG 1 cut(s) 608
HspAI GCGC 3 cut(s) 499, 629, 641
KasI GGCGCC 1 cut(s) 640
Ksp22I TGATCA 1 cut(s) 250
Kzo9I GATC 8 cut(s) 70, 166, 250, 329, 440, 535, 592, 646
LmnI GCTCC 1 cut(s) 364
Lsp1109I GCAGC 2 cut(s) 119, 141
MaeII ACGT 4 cut(s) 357, 453, 479, 531
MalI GATC 8 cut(s) 72, 168, 252, 331, 442, 537, 594, 648
MboI GATC 8 cut(s) 70, 166, 250, 329, 440, 535, 592, 646
MboII GAAGA 4 cut(s) 29, 265, 474, 539
MflI RGATCY 2 cut(s) 166, 329
MhlI GDGCHC 2 cut(s) 240, 604
MlsI TGGCCA 1 cut(s) 263
MluCI AATT 2 cut(s) 65, 81
MluNI TGGCCA 1 cut(s) 263
Mly113I GGCGCC 1 cut(s) 641
MlyI GAGTC 2 cut(s) 555, 633
MnlI CCTC 4 cut(s) 227, 338, 355, 431
Mox20I TGGCCA 1 cut(s) 263
MscI TGGCCA 1 cut(s) 263
Msp20I TGGCCA 1 cut(s) 263
MspA1I CMGCKG 1 cut(s) 132
MspI CCGG 3 cut(s) 74, 321, 555
MvnI CGCG 3 cut(s) 491, 501, 629
MwoI GCNNNNNNNGC 3 cut(s) 390, 626, 640
NarI GGCGCC 1 cut(s) 641
NcoI CCATGG 1 cut(s) 604
NdeI CATATG 1 cut(s) 369
NdeII GATC 8 cut(s) 70, 166, 250, 329, 440, 535, 592, 646
NlaIII CATG 1 cut(s) 608
NlaIV GGNNCC 3 cut(s) 7, 601, 642
PfeI GAWTC 1 cut(s) 412
PkrI GCNGC 5 cut(s) 131, 134, 503, 568, 633
PleI GAGTC 2 cut(s) 555, 632
PluTI GGCGCC 1 cut(s) 644
PpsI GAGTC 2 cut(s) 555, 632
PspFI CCCAGC 1 cut(s) 59
PspN4I GGNNCC 3 cut(s) 7, 601, 642
PspPI GGNCC 2 cut(s) 23, 335
PstNI CAGNNNCTG 1 cut(s) 132
PsuI RGATCY 2 cut(s) 166, 329
PvuII CAGCTG 1 cut(s) 132
RsaI GTAC 1 cut(s) 42
RsaNI GTAC 1 cut(s) 41
SatI GCNGC 5 cut(s) 130, 133, 502, 567, 632
Sau3AI GATC 8 cut(s) 70, 166, 250, 329, 440, 535, 592, 646
Sau96I GGNCC 2 cut(s) 23, 335
SchI GAGTC 2 cut(s) 555, 633
SduI GDGCHC 2 cut(s) 240, 604
SfoI GGCGCC 1 cut(s) 642
SmlI CTYRAG 1 cut(s) 539
SmoI CTYRAG 1 cut(s) 539
Sse9I AATT 2 cut(s) 65, 81
SsiI CCGC 7 cut(s) 9, 449, 475, 501, 515, 567, 632
SspDI GGCGCC 1 cut(s) 640
StyI CCWWGG 1 cut(s) 604
TaaI ACNGT 3 cut(s) 141, 245, 313
TaiI ACGT 4 cut(s) 360, 456, 482, 534
TaqI TCGA 2 cut(s) 69, 534
TasI AATT 2 cut(s) 65, 81
TauI GCSGC 3 cut(s) 504, 569, 634
TfiI GAWTC 1 cut(s) 412
TseI GCWGC 2 cut(s) 129, 132
TspDTI ATGAA 1 cut(s) 30
TspGWI ACGGA 1 cut(s) 602
XagI CCTNNNNNAGG 2 cut(s) 30, 342
XapI RAATTY 1 cut(s) 65
XcmI CCANNNNNNNNNTGG 1 cut(s) 416
XmiI GTMKAC 1 cut(s) 123
ZraI GACGTC 2 cut(s) 480, 532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.