Rmu_sc0006186.1_g000002

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006186.1
OG0005377
Physical Location & Seq
Forward (+)
11544 .. 12510
967 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 792 bp
atgggcttaaatctgcagatacaggagctagggcgtttggtttgcaatttggtgacagtggttcctgaaggaatcgttgtcttcttctcttcatttgattatgaaggagaggtgtacaatgcatgggaggcttcaggcatcctcgatagaattaagaagaagaagcgtctatttagagagcctaggaagaatacatatgtagaatctgttctgaaggagtacaaggaaacaattgataccttgtcttcaggagaaaggaaagaaaatccttctcagagtggtgccatacttcttgctgttgttggtggtaagatatcggagggcatcaacttgagtgatgggatgggtcggtgtatagtcatggttggactgccctatgcgagcccttctgatgttgagctgatggagagggtgaagcacattgaaagattgggaaattcaaattcgattaagatgaccaattctttacgtggtaatgaactttacagtggagaagcacatgaaggatttgatatcctccgaagttgcagacacagaggaaaagaatattatgagaatctttgcatgaaagctgtaaaccagtccatcggtagagcaatccggcatataaatgattatgcagcaattttactagttgatacacgctatgcagctgactcctcaacacggaatttcaggcatccaactagcaagctcccacagtggataaaggatcgttttgttgcttcaggagattacggccaacttcataggatgttgcatcagttttttagttataacaaaaagagatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEAD_2 Helicase_C_2

Protein Analysis

263

Amino Acids

29.92

Weight (kDa)

9.16

Isoelectric Point (pI)

38.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helicase_C_2 PF13307 15 - 226 8.9e-44 Helicase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 777
AccB1I GGYRCC 1 cut(s) 281
AclWI GGATC 1 cut(s) 720
AcoI YGGCCR 1 cut(s) 739
AcsI RAATTY 3 cut(s) 436, 442, 670
AcuI CTGAAG 5 cut(s) 87, 117, 231, 233, 711
AfaI GTAC 2 cut(s) 116, 221
AfiI CCNNNNNNNGG 1 cut(s) 666
AgsI TTSAA 2 cut(s) 425, 441
AhlI ACTAGT 1 cut(s) 631
AluBI AGCT 5 cut(s) 28, 400, 572, 653, 694
AluI AGCT 5 cut(s) 28, 400, 572, 653, 694
AlwI GGATC 1 cut(s) 720
AoxI GGCC 1 cut(s) 739
ApeKI GCWGC 2 cut(s) 620, 650
ApoI RAATTY 3 cut(s) 436, 442, 670
Asp700I GAANNNNTTC 1 cut(s) 207
AspA2I CCTAGG 1 cut(s) 182
AsuHPI GGTGA 2 cut(s) 64, 424
AvrII CCTAGG 1 cut(s) 182
BanI GGYRCC 1 cut(s) 281
BanII GRGCYC 1 cut(s) 386
BbsI GAAGAC 2 cut(s) 73, 237
BbvI GCAGC 2 cut(s) 632, 662
BccI CCATC 4 cut(s) 332, 337, 397, 593
BceAI ACGGC 1 cut(s) 754
BcuI ACTAGT 1 cut(s) 631
BfaI CTAG 4 cut(s) 29, 183, 632, 687
BfmI CTRYAG 1 cut(s) 14
BisI GCNGC 2 cut(s) 621, 651
BlnI CCTAGG 1 cut(s) 182
BlsI GCNGC 2 cut(s) 622, 652
BmiI GGNNCC 2 cut(s) 63, 283
BmsI GCATC 4 cut(s) 147, 333, 688, 769
BpiI GAAGAC 2 cut(s) 73, 237
BpuEI CTTGAG 1 cut(s) 352
BsaAI YACGTR 1 cut(s) 470
BsaJI CCNNGG 1 cut(s) 182
Bsc4I CCNNNNNNNGG 1 cut(s) 666
Bse1I ACTGG 1 cut(s) 580
BseDI CCNNGG 1 cut(s) 182
BseGI GGATG 4 cut(s) 138, 348, 679, 759
BseLI CCNNNNNNNGG 1 cut(s) 666
BseMII CTCAG 1 cut(s) 287
BseNI ACTGG 1 cut(s) 580
BseRI GAGGAG 1 cut(s) 649
BseXI GCAGC 2 cut(s) 632, 662
BshFI GGCC 1 cut(s) 741
BshNI GGYRCC 1 cut(s) 281
BsiSI CCGG 1 cut(s) 601
BslI CCNNNNNNNGG 1 cut(s) 666
BsnI GGCC 1 cut(s) 741
Bsp1286I GDGCHC 1 cut(s) 386
Bsp1407I TGTACA 1 cut(s) 114
Bsp143I GATC 1 cut(s) 712
BspANI GGCC 1 cut(s) 741
BspCNI CTCAG 1 cut(s) 286
BspLI GGNNCC 2 cut(s) 63, 283
BspMAI CTGCAG 1 cut(s) 18
BspPI GGATC 1 cut(s) 720
BspT107I GGYRCC 1 cut(s) 281
BsrGI TGTACA 1 cut(s) 114
BsrI ACTGG 1 cut(s) 580
BssECI CCNNGG 1 cut(s) 182
BssMI GATC 1 cut(s) 712
BssT1I CCWWGG 1 cut(s) 182
Bst4CI ACNGT 3 cut(s) 58, 488, 702
Bst6I CTCTTC 1 cut(s) 94
BstAUI TGTACA 1 cut(s) 114
BstBAI YACGTR 1 cut(s) 470
BstC8I GCNNGC 2 cut(s) 382, 692
BstDEI CTNAG 1 cut(s) 273
BstF5I GGATG 4 cut(s) 138, 348, 679, 759
BstKTI GATC 1 cut(s) 715
BstMBI GATC 1 cut(s) 712
BstMWI GCNNNNNNNGC 1 cut(s) 128
BstSFI CTRYAG 1 cut(s) 14
BstV1I GCAGC 2 cut(s) 632, 662
BstV2I GAAGAC 2 cut(s) 73, 237
BsuRI GGCC 1 cut(s) 741
BtsCI GGATG 4 cut(s) 138, 348, 679, 759
BtsIMutI CAGTG 3 cut(s) 63, 493, 707
Cac8I GCNNGC 2 cut(s) 382, 692
CseI GACGC 1 cut(s) 155
Csp6I GTAC 2 cut(s) 115, 220
CviAII CATG 4 cut(s) 123, 361, 500, 565
CviQI GTAC 2 cut(s) 115, 220
DdeI CTNAG 1 cut(s) 273
DpnI GATC 1 cut(s) 714
DpnII GATC 1 cut(s) 712
EaeI YGGCCR 1 cut(s) 739
Eam1104I CTCTTC 1 cut(s) 94
EarI CTCTTC 1 cut(s) 94
Eco130I CCWWGG 1 cut(s) 182
Eco24I GRGCYC 1 cut(s) 386
Eco32I GATATC 2 cut(s) 315, 514
Eco57I CTGAAG 5 cut(s) 87, 117, 231, 233, 711
EcoRV GATATC 2 cut(s) 315, 514
EcoT14I CCWWGG 1 cut(s) 182
EcoT22I ATGCAT 1 cut(s) 124
EcoT38I GRGCYC 1 cut(s) 386
ErhI CCWWGG 1 cut(s) 182
FaeI CATG 4 cut(s) 126, 364, 503, 568
FatI CATG 4 cut(s) 122, 360, 499, 564
FauNDI CATATG 1 cut(s) 196
Fnu4HI GCNGC 2 cut(s) 621, 651
FokI GGATG 4 cut(s) 125, 355, 666, 766
FriOI GRGCYC 1 cut(s) 386
Fsp4HI GCNGC 2 cut(s) 621, 651
FspBI CTAG 4 cut(s) 29, 183, 632, 687
GluI GCNGC 2 cut(s) 621, 651
HaeIII GGCC 1 cut(s) 741
HapII CCGG 1 cut(s) 601
HgaI GACGC 1 cut(s) 155
Hin1II CATG 4 cut(s) 126, 364, 503, 568
HinfI GANTC 4 cut(s) 72, 203, 556, 656
HpaII CCGG 1 cut(s) 601
HphI GGTGA 2 cut(s) 64, 424
Hpy166II GTNNAC 2 cut(s) 115, 577
Hpy188I TCNGA 5 cut(s) 213, 276, 319, 391, 521
Hpy188III TCNNGA 3 cut(s) 65, 249, 729
Hpy8I GTNNAC 2 cut(s) 115, 577
HpyAV CCTTC 6 cut(s) 62, 98, 208, 279, 396, 497
HpyCH4III ACNGT 3 cut(s) 58, 488, 702
HpyCH4IV ACGT 1 cut(s) 469
HpyCH4V TGCA 8 cut(s) 16, 45, 122, 528, 564, 620, 650, 760
HpyF10VI GCNNNNNNNGC 1 cut(s) 128
HpyF3I CTNAG 1 cut(s) 273
HpySE526I ACGT 1 cut(s) 469
Hsp92II CATG 4 cut(s) 126, 364, 503, 568
Kzo9I GATC 1 cut(s) 712
LmnI GCTCC 2 cut(s) 25, 699
LpnPI CCDG 8 cut(s) 8, 78, 120, 234, 593, 614, 661, 714
Lsp1109I GCAGC 2 cut(s) 632, 662
LweI GCATC 4 cut(s) 147, 333, 688, 769
MaeI CTAG 4 cut(s) 29, 183, 632, 687
MaeII ACGT 1 cut(s) 469
MaeIII GTNAC 1 cut(s) 52
MalI GATC 1 cut(s) 714
MboI GATC 1 cut(s) 712
MboII GAAGA 7 cut(s) 73, 76, 81, 169, 172, 199, 237
MfeI CAATTG 1 cut(s) 231
MhlI GDGCHC 1 cut(s) 386
MluCI AATT 8 cut(s) 46, 150, 231, 436, 442, 460, 624, 670
MlyI GAGTC 1 cut(s) 650
MmeI TCCRAC 2 cut(s) 346, 707
MnlI CCTC 8 cut(s) 103, 121, 152, 313, 402, 527, 530, 670
Mph1103I ATGCAT 1 cut(s) 124
MroXI GAANNNNTTC 1 cut(s) 207
MseI TTAA 3 cut(s) 8, 153, 450
MslI CAYNNNNRTG 1 cut(s) 609
MspA1I CMGCKG 1 cut(s) 653
MspI CCGG 1 cut(s) 601
MunI CAATTG 1 cut(s) 231
MwoI GCNNNNNNNGC 1 cut(s) 128
NdeI CATATG 1 cut(s) 196
NdeII GATC 1 cut(s) 712
NlaIII CATG 4 cut(s) 126, 364, 503, 568
NlaIV GGNNCC 2 cut(s) 63, 283
NmuCI GTSAC 1 cut(s) 52
NsiI ATGCAT 1 cut(s) 124
PdmI GAANNNNTTC 1 cut(s) 207
PfeI GAWTC 3 cut(s) 72, 203, 556
PkrI GCNGC 2 cut(s) 622, 652
PleI GAGTC 1 cut(s) 650
PpsI GAGTC 1 cut(s) 650
Ppu21I YACGTR 1 cut(s) 470
PsiI TTATAA 1 cut(s) 777
PspN4I GGNNCC 2 cut(s) 63, 283
PsrI GAACNNNNNNTAC 2 cut(s) 192, 224
PstI CTGCAG 1 cut(s) 18
PvuII CAGCTG 1 cut(s) 653
RsaI GTAC 2 cut(s) 116, 221
RsaNI GTAC 2 cut(s) 115, 220
RseI CAYNNNNRTG 1 cut(s) 609
SaqAI TTAA 3 cut(s) 8, 153, 450
SatI GCNGC 2 cut(s) 621, 651
Sau3AI GATC 1 cut(s) 712
SchI GAGTC 1 cut(s) 650
SduI GDGCHC 1 cut(s) 386
SetI ASST 8 cut(s) 30, 114, 242, 402, 472, 574, 655, 696
SfaNI GCATC 4 cut(s) 147, 333, 688, 769
SfcI CTRYAG 1 cut(s) 14
SmiMI CAYNNNNRTG 1 cut(s) 609
SmlI CTYRAG 1 cut(s) 331
SmoI CTYRAG 1 cut(s) 331
SpeI ACTAGT 1 cut(s) 631
Sse9I AATT 8 cut(s) 46, 150, 231, 436, 442, 460, 624, 670
SspI AATATT 1 cut(s) 548
SspMI CTAG 4 cut(s) 29, 183, 632, 687
StyI CCWWGG 1 cut(s) 182
TaaI ACNGT 3 cut(s) 58, 488, 702
TaiI ACGT 1 cut(s) 472
TaqI TCGA 2 cut(s) 144, 446
TasI AATT 8 cut(s) 46, 150, 231, 436, 442, 460, 624, 670
TatI WGTACW 2 cut(s) 114, 219
TfiI GAWTC 3 cut(s) 72, 203, 556
Tru1I TTAA 3 cut(s) 8, 153, 450
Tru9I TTAA 3 cut(s) 8, 153, 450
TscAI CASTG 3 cut(s) 63, 493, 707
TseFI GTSAC 1 cut(s) 52
TseI GCWGC 2 cut(s) 620, 650
Tsp45I GTSAC 1 cut(s) 52
TspDTI ATGAA 6 cut(s) 81, 117, 492, 516, 581, 737
TspGWI ACGGA 1 cut(s) 682
TspRI CASTG 3 cut(s) 63, 493, 707
XapI RAATTY 3 cut(s) 436, 442, 670
XmaJI CCTAGG 1 cut(s) 182
XmnI GAANNNNTTC 1 cut(s) 207
XspI CTAG 4 cut(s) 29, 183, 632, 687
Zsp2I ATGCAT 1 cut(s) 124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.