Rmu_ssc0000117.1_g000011

DNA-binding protein ESCAROLA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000117.1
OG0006163
Physical Location & Seq
Reverse (-)
34002 .. 38085
4084 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1101 bp
atgtcaggatctgagacgggagtgatgaccagtagagagccgtttctgcaggctagtcctctccagtcactgactcagtcacagtcacagagcatgcgcttggactacacgtctggcactaatgctatctataagcccgtcaccgccgccgcctcaccgccgccttaccagtccactgctggagctgctgctccgggggacagttcaactggtgctggtgctgttatgtcatctgggcagcttaacatgaacatgggtggtgagccaatgaagaggaagagagggaggccgaggaagtatgggccagatgggacaatgtcattggctctgtcaccttcagcacagtctgctgtccctgttaaccagtccagtggaggcttttctcctcctcctccgccaccgcctcctgctgctgcttctggaggcgctgcctcgcccactacttccaccaagaaagccagaggcaggccgcctggttctacgaatacaaagaagcagcaattggaagctttgggatctgcaggcatgggatttacaccacatgtcatcactgttaaagctggagaggatgtatcttcaaaaattatgtcgttctctcagaatggtcctagggctgtttgtatcttgtcagcaaatggagcgatatcaaatgtgactcttcgccaagcagccacatctggtggaactgttacatatgaggggcggtttgaaattttatcactttctggctcatttcttctatctgaaattgatggccagcgcagcagaactggaggattaagtgtctcactgtctagctcagatggtcgagttttgggtggttgtgtggcaggtctcctgactgctgcctgtcctgttcaggtagttgttgggagctttgctgcagatggtcgaaaagaaccaaagacggaaatgaaaaaggatccttcatctgcaggaccacggcttgcagcagctagcgggccaaacggggtcagtagtccaccgtcacatggaactctcagtgaatcctcaggtgggcagggaagtccacttaaccagagtacaggagccggcaataacaataacccacaaggcatgtcaagcatgtcctggaagtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

366

Amino Acids

36.69

Weight (kDa)

9.85

Isoelectric Point (pI)

62.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 179 - 294 1.4e-27 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 821
AclWI GGATC 4 cut(s) 16, 523, 917, 930
AcoI YGGCCR 1 cut(s) 754
AcsI RAATTY 1 cut(s) 711
AcuI CTGAAG 1 cut(s) 321
AfaI GTAC 1 cut(s) 1045
AfiI CCNNNNNNNGG 3 cut(s) 465, 992, 1017
AflIII ACRYGT 2 cut(s) 108, 541
AgsI TTSAA 3 cut(s) 207, 579, 710
AhdI GACNNNNNGTC 1 cut(s) 109
AjiI CACGTC 1 cut(s) 111
AjnI CCWGG 2 cut(s) 472, 1091
AjuI GAANNNNNNNTTGG 2 cut(s) 485, 517
AluBI AGCT 7 cut(s) 185, 241, 509, 560, 798, 876, 956
AluI AGCT 7 cut(s) 185, 241, 509, 560, 798, 876, 956
Alw26I GTCTC 3 cut(s) 8, 790, 839
AlwI GGATC 4 cut(s) 16, 523, 917, 930
AlwNI CAGNNNCTG 2 cut(s) 11, 70
AoxI GGCC 5 cut(s) 287, 302, 467, 754, 962
ApoI RAATTY 1 cut(s) 711
ArsI GACNNNNNNTTYG 2 cut(s) 572, 604
AspA2I CCTAGG 1 cut(s) 608
AspLEI GCGC 3 cut(s) 99, 428, 762
AspS9I GGNCC 4 cut(s) 302, 605, 938, 962
AsuC2I CCSGG 1 cut(s) 195
AsuHPI GGTGA 4 cut(s) 133, 147, 272, 324
AsuNHI GCTAGC 1 cut(s) 956
AvaII GGWCC 2 cut(s) 605, 938
AvrII CCTAGG 1 cut(s) 608
AxyI CCTNAGG 1 cut(s) 1012
BalI TGGCCA 1 cut(s) 756
BamHI GGATCC 1 cut(s) 922
BccI CCATC 4 cut(s) 302, 746, 797, 881
BceAI ACGGC 2 cut(s) 25, 959
BciT130I CCWGG 2 cut(s) 474, 1093
BcnI CCSGG 1 cut(s) 195
BcoDI GTCTC 3 cut(s) 8, 790, 839
BfaI CTAG 4 cut(s) 54, 609, 795, 957
BfmI CTRYAG 4 cut(s) 47, 519, 882, 933
BfoI RGCGCY 1 cut(s) 429
BfuAI ACCTGC 1 cut(s) 821
BlnI CCTAGG 1 cut(s) 608
Bme1390I CCNGG 3 cut(s) 195, 474, 1093
Bme18I GGWCC 2 cut(s) 605, 938
BmeRI GACNNNNNGTC 1 cut(s) 109
BmgBI CACGTC 1 cut(s) 111
BmgT120I GGNCC 4 cut(s) 302, 605, 938, 962
BmiI GGNNCC 2 cut(s) 924, 1051
BmrFI CCNGG 3 cut(s) 195, 474, 1093
BmtI GCTAGC 1 cut(s) 960
BpmI CTGGAG 5 cut(s) 47, 201, 441, 582, 792
BpuMI CCSGG 1 cut(s) 195
BsaI GGTCTC 1 cut(s) 839
BsaJI CCNNGG 4 cut(s) 194, 290, 608, 941
BsaXI ACNNNNNCTCC 2 cut(s) 555, 585
Bsc4I CCNNNNNNNGG 3 cut(s) 465, 992, 1017
Bse118I RCCGGY 1 cut(s) 1052
Bse1I ACTGG 7 cut(s) 30, 64, 169, 214, 364, 369, 775
Bse21I CCTNAGG 1 cut(s) 1012
BseBI CCWGG 2 cut(s) 474, 1093
BseDI CCNNGG 4 cut(s) 194, 290, 608, 941
BseGI GGATG 1 cut(s) 574
BseLI CCNNNNNNNGG 3 cut(s) 465, 992, 1017
BseMII CTCAG 5 cut(s) 89, 611, 813, 1015, 1026
BseNI ACTGG 7 cut(s) 30, 64, 169, 214, 364, 369, 775
BseRI GAGGAG 3 cut(s) 375, 378, 381
BshFI GGCC 5 cut(s) 289, 304, 469, 756, 964
BsiSI CCGG 2 cut(s) 194, 1053
BslFI GGGAC 3 cut(s) 212, 325, 338
BslI CCNNNNNNNGG 3 cut(s) 465, 992, 1017
BsmAI GTCTC 3 cut(s) 8, 790, 839
BsmBI CGTCTC 1 cut(s) 8
BsmFI GGGAC 3 cut(s) 212, 325, 338
BsnI GGCC 5 cut(s) 289, 304, 469, 756, 964
Bso31I GGTCTC 1 cut(s) 839
Bsp143I GATC 3 cut(s) 8, 515, 922
BspANI GGCC 5 cut(s) 289, 304, 469, 756, 964
BspCNI CTCAG 6 cut(s) 4, 88, 610, 812, 1014, 1025
BspLI GGNNCC 2 cut(s) 924, 1051
BspMAI CTGCAG 4 cut(s) 51, 523, 886, 937
BspMI ACCTGC 1 cut(s) 821
BspOI GCTAGC 1 cut(s) 960
BspPI GGATC 4 cut(s) 16, 523, 917, 930
BspTNI GGTCTC 1 cut(s) 839
BsrFI RCCGGY 1 cut(s) 1052
BsrI ACTGG 7 cut(s) 30, 64, 169, 214, 364, 369, 775
BssAI RCCGGY 1 cut(s) 1052
BssECI CCNNGG 4 cut(s) 194, 290, 608, 941
BssMI GATC 3 cut(s) 8, 515, 922
BssT1I CCWWGG 1 cut(s) 608
Bst2UI CCWGG 2 cut(s) 474, 1093
Bst4CI ACNGT 7 cut(s) 84, 203, 345, 553, 688, 792, 987
Bst6I CTCTTC 3 cut(s) 266, 272, 663
BstAPI GCANNNNNTGC 1 cut(s) 347
BstC8I GCNNGC 9 cut(s) 51, 95, 467, 523, 758, 948, 958, 962, 1054
BstDEI CTNAG 6 cut(s) 12, 75, 597, 799, 1001, 1012
BstDSI CCRYGG 1 cut(s) 941
BstF5I GGATG 1 cut(s) 574
BstH2I RGCGCY 1 cut(s) 429
BstHHI GCGC 3 cut(s) 99, 428, 762
BstKTI GATC 3 cut(s) 11, 518, 925
BstMAI GTCTC 3 cut(s) 8, 790, 839
BstMBI GATC 3 cut(s) 8, 515, 922
BstMWI GCNNNNNNNGC 6 cut(s) 46, 185, 347, 638, 762, 1083
BstNI CCWGG 2 cut(s) 474, 1093
BstNSI RCATGY 4 cut(s) 97, 545, 1081, 1090
BstSCI CCNGG 3 cut(s) 193, 472, 1091
BstSFI CTRYAG 4 cut(s) 47, 519, 882, 933
BstX2I RGATCY 3 cut(s) 8, 515, 922
BstXI CCANNNNNNTGG 1 cut(s) 371
BstYI RGATCY 3 cut(s) 8, 515, 922
Bsu36I CCTNAGG 1 cut(s) 1012
BsuRI GGCC 5 cut(s) 289, 304, 469, 756, 964
BtgI CCRYGG 1 cut(s) 941
BtrI CACGTC 1 cut(s) 111
BtsCI GGATG 1 cut(s) 574
BtsI GCAGTG 1 cut(s) 174
BtsIMutI CAGTG 6 cut(s) 68, 174, 376, 549, 788, 1009
BveI ACCTGC 1 cut(s) 821
Cac8I GCNNGC 9 cut(s) 51, 95, 467, 523, 758, 948, 958, 962, 1054
CaiI CAGNNNCTG 2 cut(s) 11, 70
CfoI GCGC 3 cut(s) 99, 428, 762
Cfr10I RCCGGY 1 cut(s) 1052
Cfr13I GGNCC 4 cut(s) 302, 605, 938, 962
Csp6I GTAC 1 cut(s) 1044
CviAII CATG 8 cut(s) 94, 247, 253, 526, 542, 992, 1078, 1087
CviQI GTAC 1 cut(s) 1044
DdeI CTNAG 6 cut(s) 12, 75, 597, 799, 1001, 1012
DpnI GATC 3 cut(s) 10, 517, 924
DpnII GATC 3 cut(s) 8, 515, 922
DriI GACNNNNNGTC 1 cut(s) 109
EaeI YGGCCR 1 cut(s) 754
Eam1104I CTCTTC 3 cut(s) 266, 272, 663
Eam1105I GACNNNNNGTC 1 cut(s) 109
EarI CTCTTC 3 cut(s) 266, 272, 663
EciI GGCGGA 1 cut(s) 384
Eco130I CCWWGG 1 cut(s) 608
Eco31I GGTCTC 1 cut(s) 839
Eco32I GATATC 1 cut(s) 645
Eco47I GGWCC 2 cut(s) 605, 938
Eco57I CTGAAG 1 cut(s) 321
Eco81I CCTNAGG 1 cut(s) 1012
EcoRII CCWGG 2 cut(s) 472, 1091
EcoRV GATATC 1 cut(s) 645
EcoT14I CCWWGG 1 cut(s) 608
ErhI CCWWGG 1 cut(s) 608
Esp3I CGTCTC 1 cut(s) 8
FaeI CATG 8 cut(s) 97, 250, 256, 529, 545, 995, 1081, 1090
FaqI GGGAC 3 cut(s) 212, 325, 338
FatI CATG 8 cut(s) 93, 246, 252, 525, 541, 991, 1077, 1086
FauI CCCGC 1 cut(s) 953
FauNDI CATATG 1 cut(s) 694
FokI GGATG 1 cut(s) 581
FspBI CTAG 4 cut(s) 54, 609, 795, 957
GlaI GCGC 3 cut(s) 98, 427, 761
GsuI CTGGAG 5 cut(s) 47, 201, 441, 582, 792
HaeII RGCGCY 1 cut(s) 429
HaeIII GGCC 5 cut(s) 289, 304, 469, 756, 964
HapII CCGG 2 cut(s) 194, 1053
HhaI GCGC 3 cut(s) 99, 428, 762
Hin1II CATG 8 cut(s) 97, 250, 256, 529, 545, 995, 1081, 1090
Hin6I GCGC 3 cut(s) 97, 426, 760
HinP1I GCGC 3 cut(s) 97, 426, 760
HincII GTYRAC 1 cut(s) 361
HindII GTYRAC 1 cut(s) 361
HindIII AAGCTT 1 cut(s) 507
HinfI GANTC 3 cut(s) 73, 655, 1007
HpaI GTTAAC 1 cut(s) 361
HpaII CCGG 2 cut(s) 194, 1053
HphI GGTGA 4 cut(s) 133, 147, 272, 324
Hpy166II GTNNAC 4 cut(s) 174, 361, 983, 1031
Hpy188I TCNGA 4 cut(s) 13, 600, 745, 802
Hpy188III TCNNGA 3 cut(s) 6, 420, 838
Hpy8I GTNNAC 4 cut(s) 174, 361, 983, 1031
HpyAV CCTTC 2 cut(s) 345, 936
HpyCH4III ACNGT 7 cut(s) 84, 203, 345, 553, 688, 792, 987
HpyCH4IV ACGT 1 cut(s) 110
HpyCH4V TGCA 5 cut(s) 49, 521, 884, 935, 950
HpyF10VI GCNNNNNNNGC 6 cut(s) 46, 185, 347, 638, 762, 1083
HpyF3I CTNAG 6 cut(s) 12, 75, 597, 799, 1001, 1012
HpySE526I ACGT 1 cut(s) 110
Hsp92II CATG 8 cut(s) 97, 250, 256, 529, 545, 995, 1081, 1090
HspAI GCGC 3 cut(s) 97, 426, 760
KroI GCCGGC 1 cut(s) 1052
KroNI GCCGGC 1 cut(s) 1054
KspAI GTTAAC 1 cut(s) 361
Kzo9I GATC 3 cut(s) 8, 515, 922
LmnI GCTCC 5 cut(s) 182, 196, 638, 873, 1049
MaeI CTAG 4 cut(s) 54, 609, 795, 957
MaeII ACGT 1 cut(s) 110
MaeIII GTNAC 8 cut(s) 66, 78, 84, 139, 330, 652, 688, 987
MalI GATC 3 cut(s) 10, 517, 924
MboI GATC 3 cut(s) 8, 515, 922
MboII GAAGA 5 cut(s) 283, 289, 567, 650, 728
MfeI CAATTG 1 cut(s) 500
MflI RGATCY 3 cut(s) 8, 515, 922
MlsI TGGCCA 1 cut(s) 756
MluCI AATT 4 cut(s) 500, 582, 711, 747
MluNI TGGCCA 1 cut(s) 756
MlyI GAGTC 2 cut(s) 67, 649
Mox20I TGGCCA 1 cut(s) 756
MroNI GCCGGC 1 cut(s) 1052
MscI TGGCCA 1 cut(s) 756
MseI TTAA 5 cut(s) 243, 360, 555, 779, 1035
MslI CAYNNNNRTG 1 cut(s) 251
Msp20I TGGCCA 1 cut(s) 756
MspI CCGG 2 cut(s) 194, 1053
MspR9I CCNGG 3 cut(s) 195, 474, 1093
MunI CAATTG 1 cut(s) 500
MvaI CCWGG 2 cut(s) 474, 1093
MwoI GCNNNNNNNGC 6 cut(s) 46, 185, 347, 638, 762, 1083
NaeI GCCGGC 1 cut(s) 1054
NciI CCSGG 1 cut(s) 195
NdeI CATATG 1 cut(s) 694
NdeII GATC 3 cut(s) 8, 515, 922
NgoMIV GCCGGC 1 cut(s) 1052
NheI GCTAGC 1 cut(s) 956
NlaIII CATG 8 cut(s) 97, 250, 256, 529, 545, 995, 1081, 1090
NlaIV GGNNCC 2 cut(s) 924, 1051
NmeAIII GCCGAG 1 cut(s) 315
NmuCI GTSAC 7 cut(s) 66, 78, 84, 139, 330, 652, 987
NspI RCATGY 4 cut(s) 97, 545, 1081, 1090
PaeI GCATGC 1 cut(s) 97
PciI ACATGT 1 cut(s) 541
PdiI GCCGGC 1 cut(s) 1054
PfeI GAWTC 1 cut(s) 1007
PflFI GACNNNGTC 2 cut(s) 76, 316
PfoI TCCNGGA 1 cut(s) 1091
PleI GAGTC 2 cut(s) 67, 649
PpsI GAGTC 2 cut(s) 67, 649
PscI ACATGT 1 cut(s) 541
Psp6I CCWGG 2 cut(s) 472, 1091
PspGI CCWGG 2 cut(s) 472, 1091
PspN4I GGNNCC 2 cut(s) 924, 1051
PspPI GGNCC 4 cut(s) 302, 605, 938, 962
PstI CTGCAG 4 cut(s) 51, 523, 886, 937
PstNI CAGNNNCTG 2 cut(s) 11, 70
PsuI RGATCY 3 cut(s) 8, 515, 922
PsyI GACNNNGTC 2 cut(s) 76, 316
RsaI GTAC 1 cut(s) 1045
RsaNI GTAC 1 cut(s) 1044
RseI CAYNNNNRTG 1 cut(s) 251
SaqAI TTAA 5 cut(s) 243, 360, 555, 779, 1035
Sau3AI GATC 3 cut(s) 8, 515, 922
Sau96I GGNCC 4 cut(s) 302, 605, 938, 962
SchI GAGTC 2 cut(s) 67, 649
ScrFI CCNGG 3 cut(s) 195, 474, 1093
SfcI CTRYAG 4 cut(s) 47, 519, 882, 933
SinI GGWCC 2 cut(s) 605, 938
SmiMI CAYNNNNRTG 1 cut(s) 251
SphI GCATGC 1 cut(s) 97
Sse9I AATT 4 cut(s) 500, 582, 711, 747
SspMI CTAG 4 cut(s) 54, 609, 795, 957
StyD4I CCNGG 3 cut(s) 193, 472, 1091
StyI CCWWGG 1 cut(s) 608
TaaI ACNGT 7 cut(s) 84, 203, 345, 553, 688, 792, 987
TaiI ACGT 1 cut(s) 113
TaqI TCGA 2 cut(s) 808, 892
TasI AATT 4 cut(s) 500, 582, 711, 747
TatI WGTACW 1 cut(s) 1043
TauI GCSGC 4 cut(s) 149, 152, 163, 472
TfiI GAWTC 1 cut(s) 1007
Tru1I TTAA 5 cut(s) 243, 360, 555, 779, 1035
Tru9I TTAA 5 cut(s) 243, 360, 555, 779, 1035
TscAI CASTG 6 cut(s) 75, 181, 376, 556, 795, 1009
TseFI GTSAC 7 cut(s) 66, 78, 84, 139, 330, 652, 987
Tsp45I GTSAC 7 cut(s) 66, 78, 84, 139, 330, 652, 987
TspDTI ATGAA 4 cut(s) 263, 284, 918, 929
TspGWI ACGGA 1 cut(s) 923
TspRI CASTG 6 cut(s) 75, 181, 376, 556, 795, 1009
Tth111I GACNNNGTC 2 cut(s) 76, 316
VpaK11BI GGWCC 2 cut(s) 605, 938
XapI RAATTY 1 cut(s) 711
XceI RCATGY 4 cut(s) 97, 545, 1081, 1090
XcmI CCANNNNNNNNNTGG 1 cut(s) 176
XmaJI CCTAGG 1 cut(s) 608
XspI CTAG 4 cut(s) 54, 609, 795, 957
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.