Rmu_ssc0000133.1_g000001

DUF761-associated sequence motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000133.1
OG0009893
Physical Location & Seq
Forward (+)
1 .. 4182
4182 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1454 bp
gggcagcaggcgtgcgacgatgctgcaggggcagtaggaggcacacgggtgctcaagggctgcaggagcatcgcacggggcagcaggcgtgcgacgatgctgcaggggcagtaggaggcacacgggtgctcaagggttgcaagggcagtaggaggcacacgggtgctcaagggctgcaggagcatcgcacgtgtctatcttcccacataacaaaaccctttgccggttatcggttcaacttcgacatgaagttgctgtcttcttctgaatcctctcctttttcttctttttcttcgtgttcatcatccaccaccactaccaccacttttgatctaaccaattcaaaaggtgctactaatggatgcattgctggcattttgcgcagaattctctgctatggtagcttgcctacatacccttctgatcatatctcagaagaaaccaattctgtcgatcaatctcaaaatgatcgagcattcaagtcgaaaaacaaaacaggggaaacagaggcatctgccactccaagcttagtggcaaggctaatggggttggagtcaattccagatgccaacttggtctccaccccaaattcaatatcccgtagccggtctatgaattctgtggaccggctttcgggagaccatgatccaatcgaggcacatgagcgccgacgagtcaagtcgtttagagagatgccaacatttattgaaaatgaggaattccttgttcttagctttgagaatgggagtgaaagaaaagaaaggagatcgaaggggagcaaaaatgagacgggccatggagaattgaggaagaagaaggcaggaaagagcagaaacaagggtaatagaaaacaaagagggtttgagaaggaaaagaagcaagttcaagaagaaaaaagcagaagggttttgaaagatttgaatgggagggaaatcctaagaagcaaaacctctcataaacccaagaaagagaccatctttgatatgcttaagaatgtagaatctgattgtacctcggaagatttaagcccggtttctgtccttgattgtggtcagtttctagttgataacgaagttcctacatcagaagaagatccagtcatggtgagttcatttccgagaagaaaagacgactgtttaacttgtgatgcaagaaaaacaaagaaaatcgaaggcatatgtcttggagcaaagaaagagtatcatactgcagagtactttgagattttgggtgaaatttgtactctgactgaagctgaattggttggatcaaattggagcgtacacaaaggcatctggaataatcatgaaggttctgcaggaattgctgcagatttcgagtcacaaattctaggccaattaatagaggagctcgtggatcaactcgctgattttcctactgaatattcaaaacctgtaagatttacgaccattgtaaatttggtattactataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VARLMGL

Protein Analysis

483

Amino Acids

53.56

Weight (kDa)

8.65

Isoelectric Point (pI)

57.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VARLMGL PF14383 173 - 190 9.8e-08 DUF761-associated sequence motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009893)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G58630
fragaria_vesca FvH4_3g38760 FvH4_3g38760
malus_domestica MD03G1068300.v1.1 MD11G1072700.v1.1
prunus_persica Prupe.6G056200_v2.0.a1
pyrus_communis pycom03g05470 pycom03g05480 pycom11g06040
rosa_chinensis RchiOBHm_Chr5g0069911
rosa_laevigata RLG00000036107
rosa_multiflora Rmu_ssc0000133.1_g000001
rosa_roxburghii Rroxscaffold_1G00011150
rosa_rugosa Rorug05G0400700
rosa_samantha Rh5AG457800 Rh5BG476600 Rh5BG476700 Rh5CG500400 Rh5CG500500 Rh5DG488500
rosa_wichuraiana Rw5G042750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 382
AclWI GGATC 4 cut(s) 640, 1087, 1275, 1385
AcsI RAATTY 7 cut(s) 386, 588, 615, 718, 1235, 1346, 1437
AcuI CTGAAG 1 cut(s) 1271
AcvI CACGTG 1 cut(s) 191
AfaI GTAC 4 cut(s) 1011, 1216, 1242, 1283
AfiI CCNNNNNNNGG 4 cut(s) 223, 230, 605, 633
AflII CTTAAG 1 cut(s) 988
AflIII ACRYGT 1 cut(s) 190
AgsI TTSAA 9 cut(s) 237, 344, 480, 593, 709, 886, 912, 921, 1409
AleI CACNNNNGTG 3 cut(s) 47, 124, 161
AluBI AGCT 5 cut(s) 404, 527, 734, 1255, 1371
AluI AGCT 5 cut(s) 404, 527, 734, 1255, 1371
Alw21I GWGCWC 4 cut(s) 54, 131, 168, 1373
Alw26I GTCTC 4 cut(s) 582, 632, 781, 964
AlwI GGATC 4 cut(s) 640, 1087, 1275, 1385
AoxI GGCC 2 cut(s) 792, 1353
ApeKI GCWGC 7 cut(s) 4, 23, 60, 81, 100, 174, 1327
ApoI RAATTY 7 cut(s) 386, 588, 615, 718, 1235, 1346, 1437
AseI ATTAAT 1 cut(s) 1360
AspLEI GCGC 2 cut(s) 383, 668
AspS9I GGNCC 2 cut(s) 624, 792
AsuC2I CCSGG 1 cut(s) 1030
AsuHPI GGTGA 2 cut(s) 1116, 1243
AvaII GGWCC 1 cut(s) 624
BanII GRGCYC 1 cut(s) 1373
BauI CACGAG 1 cut(s) 1372
BbrPI CACGTG 1 cut(s) 191
BbsI GAAGAC 1 cut(s) 251
Bbv12I GWGCWC 4 cut(s) 54, 131, 168, 1373
BbvI GCAGC 7 cut(s) 10, 16, 47, 87, 93, 161, 1314
BccI CCATC 1 cut(s) 982
BclI TGATCA 1 cut(s) 423
BcnI CCSGG 1 cut(s) 1030
BcoDI GTCTC 4 cut(s) 582, 632, 781, 964
BfaI CTAG 2 cut(s) 1060, 1351
BfmI CTRYAG 7 cut(s) 24, 61, 101, 175, 1208, 1316, 1328
BfoI RGCGCY 1 cut(s) 669
BfrI CTTAAG 1 cut(s) 988
BisI GCNGC 7 cut(s) 5, 24, 61, 82, 101, 175, 1328
BlsI GCNGC 7 cut(s) 6, 25, 62, 83, 102, 176, 1329
BmcAI AGTACT 1 cut(s) 1216
Bme1390I CCNGG 1 cut(s) 1030
Bme18I GGWCC 1 cut(s) 624
BmgT120I GGNCC 2 cut(s) 624, 792
BmrFI CCNGG 1 cut(s) 1030
BpiI GAAGAC 1 cut(s) 251
BpuEI CTTGAG 3 cut(s) 38, 115, 152
BpuMI CCSGG 1 cut(s) 1030
BsaAI YACGTR 1 cut(s) 191
BsaI GGTCTC 3 cut(s) 582, 632, 964
BsaJI CCNNGG 2 cut(s) 795, 1013
BsaXI ACNNNNNCTCC 2 cut(s) 562, 592
Bsc4I CCNNNNNNNGG 4 cut(s) 223, 230, 605, 633
Bse118I RCCGGY 3 cut(s) 223, 605, 626
Bse1I ACTGG 1 cut(s) 1096
Bse3DI GCAATG 1 cut(s) 365
BseDI CCNNGG 2 cut(s) 795, 1013
BseGI GGATG 2 cut(s) 304, 367
BseLI CCNNNNNNNGG 4 cut(s) 223, 230, 605, 633
BseMI GCAATG 1 cut(s) 365
BseMII CTCAG 1 cut(s) 446
BseNI ACTGG 1 cut(s) 1096
BseRI GAGGAG 1 cut(s) 1381
BseXI GCAGC 7 cut(s) 10, 16, 47, 87, 93, 161, 1314
BshFI GGCC 2 cut(s) 794, 1355
BsiHKAI GWGCWC 4 cut(s) 54, 131, 168, 1373
BsiSI CCGG 4 cut(s) 224, 606, 627, 1030
BslI CCNNNNNNNGG 4 cut(s) 223, 230, 605, 633
BsmAI GTCTC 4 cut(s) 582, 632, 781, 964
BsmBI CGTCTC 1 cut(s) 781
BsmI GAATGC 1 cut(s) 475
BsnI GGCC 2 cut(s) 794, 1355
Bso31I GGTCTC 3 cut(s) 582, 632, 964
Bsp1286I GDGCHC 4 cut(s) 54, 131, 168, 1373
Bsp143I GATC 9 cut(s) 330, 423, 453, 468, 645, 766, 1092, 1267, 1377
Bsp19I CCATGG 1 cut(s) 795
BspANI GGCC 2 cut(s) 794, 1355
BspCNI CTCAG 1 cut(s) 445
BspHI TCATGA 1 cut(s) 1305
BspMAI CTGCAG 7 cut(s) 28, 65, 105, 179, 1212, 1320, 1332
BspPI GGATC 4 cut(s) 640, 1087, 1275, 1385
BspTI CTTAAG 1 cut(s) 988
BspTNI GGTCTC 3 cut(s) 582, 632, 964
BsrDI GCAATG 1 cut(s) 365
BsrFI RCCGGY 3 cut(s) 223, 605, 626
BsrI ACTGG 1 cut(s) 1096
BssAI RCCGGY 3 cut(s) 223, 605, 626
BssECI CCNNGG 2 cut(s) 795, 1013
BssMI GATC 9 cut(s) 330, 423, 453, 468, 645, 766, 1092, 1267, 1377
BssSI CACGAG 1 cut(s) 1372
BssT1I CCWWGG 1 cut(s) 795
Bst2BI CACGAG 1 cut(s) 1372
Bst4CI ACNGT 1 cut(s) 1135
BstAFI CTTAAG 1 cut(s) 988
BstAPI GCANNNNNTGC 1 cut(s) 1324
BstBAI YACGTR 1 cut(s) 191
BstC8I GCNNGC 6 cut(s) 9, 13, 86, 90, 372, 406
BstDEI CTNAG 4 cut(s) 432, 528, 730, 937
BstDSI CCRYGG 1 cut(s) 795
BstF5I GGATG 2 cut(s) 304, 367
BstH2I RGCGCY 1 cut(s) 669
BstHHI GCGC 2 cut(s) 383, 668
BstKTI GATC 9 cut(s) 333, 426, 456, 471, 648, 769, 1095, 1270, 1380
BstMAI GTCTC 4 cut(s) 582, 632, 781, 964
BstMBI GATC 9 cut(s) 330, 423, 453, 468, 645, 766, 1092, 1267, 1377
BstMWI GCNNNNNNNGC 8 cut(s) 29, 66, 106, 180, 371, 380, 401, 1324
BstSCI CCNGG 1 cut(s) 1028
BstSFI CTRYAG 7 cut(s) 24, 61, 101, 175, 1208, 1316, 1328
BstV1I GCAGC 7 cut(s) 10, 16, 47, 87, 93, 161, 1314
BstV2I GAAGAC 1 cut(s) 251
BstX2I RGATCY 1 cut(s) 1092
BstYI RGATCY 1 cut(s) 1092
BsuRI GGCC 2 cut(s) 794, 1355
BtgI CCRYGG 1 cut(s) 795
BtgZI GCGATG 2 cut(s) 55, 169
BtsCI GGATG 2 cut(s) 304, 367
Cac8I GCNNGC 6 cut(s) 9, 13, 86, 90, 372, 406
CciI TCATGA 1 cut(s) 1305
CfoI GCGC 2 cut(s) 383, 668
Cfr10I RCCGGY 3 cut(s) 223, 605, 626
Cfr13I GGNCC 2 cut(s) 624, 792
Csp6I GTAC 4 cut(s) 1010, 1215, 1241, 1282
CspCI CAANNNNNGTGG 4 cut(s) 309, 344, 512, 547
CviAII CATG 6 cut(s) 246, 643, 661, 796, 1101, 1306
CviQI GTAC 4 cut(s) 1010, 1215, 1241, 1282
DdeI CTNAG 4 cut(s) 432, 528, 730, 937
DpnI GATC 9 cut(s) 332, 425, 455, 470, 647, 768, 1094, 1269, 1379
DpnII GATC 9 cut(s) 330, 423, 453, 468, 645, 766, 1092, 1267, 1377
Ecl136II GAGCTC 1 cut(s) 1371
Eco130I CCWWGG 1 cut(s) 795
Eco24I GRGCYC 1 cut(s) 1373
Eco31I GGTCTC 3 cut(s) 582, 632, 964
Eco47I GGWCC 1 cut(s) 624
Eco53kI GAGCTC 1 cut(s) 1371
Eco57I CTGAAG 1 cut(s) 1271
Eco72I CACGTG 1 cut(s) 191
EcoICRI GAGCTC 1 cut(s) 1371
EcoRI GAATTC 3 cut(s) 386, 615, 718
EcoT14I CCWWGG 1 cut(s) 795
EcoT22I ATGCAT 1 cut(s) 367
EcoT38I GRGCYC 1 cut(s) 1373
ErhI CCWWGG 1 cut(s) 795
Esp3I CGTCTC 1 cut(s) 781
FaeI CATG 6 cut(s) 249, 646, 664, 799, 1104, 1309
FatI CATG 6 cut(s) 245, 642, 660, 795, 1100, 1305
FauNDI CATATG 1 cut(s) 1177
FbaI TGATCA 1 cut(s) 423
Fnu4HI GCNGC 7 cut(s) 5, 24, 61, 82, 101, 175, 1328
FokI GGATG 2 cut(s) 291, 374
FriOI GRGCYC 1 cut(s) 1373
Fsp4HI GCNGC 7 cut(s) 5, 24, 61, 82, 101, 175, 1328
FspBI CTAG 2 cut(s) 1060, 1351
FspI TGCGCA 1 cut(s) 382
GlaI GCGC 2 cut(s) 382, 667
GluI GCNGC 7 cut(s) 5, 24, 61, 82, 101, 175, 1328
HaeII RGCGCY 1 cut(s) 669
HaeIII GGCC 2 cut(s) 794, 1355
HapII CCGG 4 cut(s) 224, 606, 627, 1030
HhaI GCGC 2 cut(s) 383, 668
Hin1II CATG 6 cut(s) 249, 646, 664, 799, 1104, 1309
Hin6I GCGC 2 cut(s) 381, 666
HinP1I GCGC 2 cut(s) 381, 666
HindIII AAGCTT 1 cut(s) 525
HinfI GANTC 5 cut(s) 268, 553, 674, 1000, 1339
HpaII CCGG 4 cut(s) 224, 606, 627, 1030
HphI GGTGA 2 cut(s) 1116, 1243
Hpy166II GTNNAC 2 cut(s) 624, 1284
Hpy188I TCNGA 8 cut(s) 267, 423, 435, 1005, 1017, 1086, 1118, 1247
Hpy188III TCNNGA 5 cut(s) 562, 635, 886, 1296, 1306
Hpy8I GTNNAC 2 cut(s) 624, 1284
Hpy99I CGWCG 3 cut(s) 20, 97, 674
HpyAV CCTTC 7 cut(s) 428, 765, 810, 861, 896, 1165, 1303
HpyCH4III ACNGT 1 cut(s) 1135
HpyCH4IV ACGT 1 cut(s) 190
HpyF10VI GCNNNNNNNGC 8 cut(s) 29, 66, 106, 180, 371, 380, 401, 1324
HpyF3I CTNAG 4 cut(s) 432, 528, 730, 937
HpySE526I ACGT 1 cut(s) 190
Hsp92II CATG 6 cut(s) 249, 646, 664, 799, 1104, 1309
HspAI GCGC 2 cut(s) 381, 666
Ksp22I TGATCA 1 cut(s) 423
Kzo9I GATC 9 cut(s) 330, 423, 453, 468, 645, 766, 1092, 1267, 1377
LmnI GCTCC 6 cut(s) 66, 180, 776, 1186, 1277, 1368
Lsp1109I GCAGC 7 cut(s) 10, 16, 47, 87, 93, 161, 1314
MaeI CTAG 2 cut(s) 1060, 1351
MaeII ACGT 1 cut(s) 190
MaeIII GTNAC 1 cut(s) 1340
MalI GATC 9 cut(s) 332, 425, 455, 470, 647, 768, 1094, 1269, 1379
MboI GATC 9 cut(s) 330, 423, 453, 468, 645, 766, 1092, 1267, 1377
MflI RGATCY 1 cut(s) 1092
MhlI GDGCHC 4 cut(s) 54, 131, 168, 1373
MlyI GAGTC 3 cut(s) 562, 683, 1348
MmeI TCCRAC 2 cut(s) 530, 1245
Mph1103I ATGCAT 1 cut(s) 367
MseI TTAA 4 cut(s) 989, 1024, 1138, 1360
MslI CAYNNNNRTG 3 cut(s) 47, 124, 161
MspCI CTTAAG 1 cut(s) 988
MspI CCGG 4 cut(s) 224, 606, 627, 1030
MspR9I CCNGG 1 cut(s) 1030
Mva1269I GAATGC 1 cut(s) 475
MwoI GCNNNNNNNGC 8 cut(s) 29, 66, 106, 180, 371, 380, 401, 1324
NciI CCSGG 1 cut(s) 1030
NcoI CCATGG 1 cut(s) 795
NdeI CATATG 1 cut(s) 1177
NdeII GATC 9 cut(s) 330, 423, 453, 468, 645, 766, 1092, 1267, 1377
NlaIII CATG 6 cut(s) 249, 646, 664, 799, 1104, 1309
NmuCI GTSAC 1 cut(s) 1340
NsbI TGCGCA 1 cut(s) 382
NsiI ATGCAT 1 cut(s) 367
OliI CACNNNNGTG 3 cut(s) 47, 124, 161
PagI TCATGA 1 cut(s) 1305
PctI GAATGC 1 cut(s) 475
PfeI GAWTC 2 cut(s) 268, 1000
PkrI GCNGC 7 cut(s) 6, 25, 62, 83, 102, 176, 1329
PleI GAGTC 3 cut(s) 561, 682, 1347
PmaCI CACGTG 1 cut(s) 191
PmlI CACGTG 1 cut(s) 191
PpsI GAGTC 3 cut(s) 561, 682, 1347
Ppu21I YACGTR 1 cut(s) 191
PshBI ATTAAT 1 cut(s) 1360
Psp124BI GAGCTC 1 cut(s) 1373
PspCI CACGTG 1 cut(s) 191
PspPI GGNCC 2 cut(s) 624, 792
PstI CTGCAG 7 cut(s) 28, 65, 105, 179, 1212, 1320, 1332
PsuI RGATCY 1 cut(s) 1092
RsaI GTAC 4 cut(s) 1011, 1216, 1242, 1283
RsaNI GTAC 4 cut(s) 1010, 1215, 1241, 1282
RseI CAYNNNNRTG 3 cut(s) 47, 124, 161
SacI GAGCTC 1 cut(s) 1373
SaqAI TTAA 4 cut(s) 989, 1024, 1138, 1360
SatI GCNGC 7 cut(s) 5, 24, 61, 82, 101, 175, 1328
Sau3AI GATC 9 cut(s) 330, 423, 453, 468, 645, 766, 1092, 1267, 1377
Sau96I GGNCC 2 cut(s) 624, 792
ScaI AGTACT 1 cut(s) 1216
SchI GAGTC 3 cut(s) 562, 683, 1348
ScrFI CCNGG 1 cut(s) 1030
SduI GDGCHC 4 cut(s) 54, 131, 168, 1373
SfcI CTRYAG 7 cut(s) 24, 61, 101, 175, 1208, 1316, 1328
SinI GGWCC 1 cut(s) 624
SmiMI CAYNNNNRTG 3 cut(s) 47, 124, 161
SmlI CTYRAG 4 cut(s) 53, 130, 167, 988
SmoI CTYRAG 4 cut(s) 53, 130, 167, 988
SspI AATATT 1 cut(s) 1405
SspMI CTAG 2 cut(s) 1060, 1351
SstI GAGCTC 1 cut(s) 1373
StyD4I CCNGG 1 cut(s) 1028
StyI CCWWGG 1 cut(s) 795
TaaI ACNGT 1 cut(s) 1135
TaiI ACGT 1 cut(s) 193
TaqI TCGA 8 cut(s) 242, 452, 471, 484, 653, 769, 1169, 1337
TatI WGTACW 2 cut(s) 1214, 1240
TfiI GAWTC 2 cut(s) 268, 1000
Tru1I TTAA 4 cut(s) 989, 1024, 1138, 1360
Tru9I TTAA 4 cut(s) 989, 1024, 1138, 1360
TseFI GTSAC 1 cut(s) 1340
TseI GCWGC 7 cut(s) 4, 23, 60, 81, 100, 174, 1327
Tsp45I GTSAC 1 cut(s) 1340
TspDTI ATGAA 5 cut(s) 262, 290, 628, 1100, 1322
Vha464I CTTAAG 1 cut(s) 988
VpaK11BI GGWCC 1 cut(s) 624
VspI ATTAAT 1 cut(s) 1360
XapI RAATTY 7 cut(s) 386, 588, 615, 718, 1235, 1346, 1437
XcmI CCANNNNNNNNNTGG 1 cut(s) 1437
XspI CTAG 2 cut(s) 1060, 1351
ZrmI AGTACT 1 cut(s) 1216
Zsp2I ATGCAT 1 cut(s) 367
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.