Rmu_ssc0000184.1_g000039

Sucrase/ferredoxin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000184.1
OG0007599
Physical Location & Seq
Forward (+)
156425 .. 159398
2974 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 903 bp
atggctagcgcccgagaccgagacgactccttgctcccatttacgacgcccccatccacgtcgtcttccccgattgtgatatccgacccgctcgacgggttcctctccgacccgaattcccacatcgggaccagcgcctccgggagcttccagaacgagggtttgttggcagatactagcgcgagcggcagcgacgccgagtttgggttttcgaggggcgagttccggacgagtcaactcgccggcacggtggagttttatcagaggcatgtgtttttgtgctacaagaacccgcaggtgtggccgccgaggattgaggcggcggagtttgatcggctgccgaggctgctccacgcggccgtgatggcgaggagggtggatatgaggaaacagactcgcttaacaatatgtgaagggcacgatggaactgagacttcaaatggtgatgtattaattttccccgacatgatcagatacagggatcgccgttgtggagtctgtgggcctcccttgatcaatagattcagagaagagatagatttgcatggtctacaaggtaaagtgtctgttaggccatgttcacacattggggggcataagtatgcaggaaatgtaattatatttggatcaaatatcaacaaagaagtcactggccactggtatggatatgttgctccagaagatgtacctcaattgcttgagcagcatattgggagaggagaaattgtagactggctatggaggggtcaaatgggtttatcagaagaacaacagaagaaatctcaagaactaaggctccagcccaatggcgagtcaaatgtggagaaaagaaatctcaagttgaaattgggggctgttgccaggaaaacggaagctcttcttgctgccagaacacttcgttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Suc_Fer-like

Protein Analysis

300

Amino Acids

33.44

Weight (kDa)

7.12

Isoelectric Point (pI)

45.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Suc_Fer-like PF06999 160 - 249 5.4e-26 Sucrase/ferredoxin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 286
Acc36I ACCTGC 1 cut(s) 286
AccBSI CCGCTC 2 cut(s) 91, 186
AccI GTMKAC 2 cut(s) 550, 729
AccII CGCG 2 cut(s) 182, 356
AccIII TCCGGA 1 cut(s) 225
AciI CCGC 7 cut(s) 89, 186, 293, 305, 320, 323, 356
AclWI GGATC 2 cut(s) 489, 634
AcoI YGGCCR 3 cut(s) 302, 357, 652
AcsI RAATTY 1 cut(s) 115
AcyI GRCGYC 2 cut(s) 47, 195
AfaI GTAC 1 cut(s) 687
AfiI CCNNNNNNNGG 4 cut(s) 95, 126, 157, 204
AgsI TTSAA 2 cut(s) 438, 844
AjiI CACGTC 1 cut(s) 60
AjnI CCWGG 1 cut(s) 860
AloI GAACNNNNNNTCC 2 cut(s) 780, 812
AluBI AGCT 2 cut(s) 147, 875
AluI AGCT 2 cut(s) 147, 875
Alw26I GTCTC 3 cut(s) 9, 15, 425
AlwI GGATC 2 cut(s) 489, 634
Ama87I CYCGRG 1 cut(s) 12
Aor13HI TCCGGA 1 cut(s) 225
AoxI GGCC 5 cut(s) 302, 357, 503, 572, 652
ApeKI GCWGC 5 cut(s) 189, 337, 346, 703, 884
ApoI RAATTY 1 cut(s) 115
ArsI GACNNNNNNTTYG 2 cut(s) 185, 217
AseI ATTAAT 1 cut(s) 452
Asp700I GAANNNNTTC 1 cut(s) 876
AspLEI GCGC 3 cut(s) 11, 137, 182
AspS9I GGNCC 2 cut(s) 129, 503
AsuC2I CCSGG 1 cut(s) 142
AsuHPI GGTGA 1 cut(s) 455
AsuNHI GCTAGC 1 cut(s) 5
AvaI CYCGRG 1 cut(s) 12
AvaII GGWCC 1 cut(s) 129
BaeGI GKGCMC 1 cut(s) 420
BalI TGGCCA 1 cut(s) 654
BbsI GAAGAC 1 cut(s) 57
BbvI GCAGC 5 cut(s) 201, 324, 333, 715, 871
BccI CCATC 3 cut(s) 61, 358, 416
BceAI ACGGC 2 cut(s) 344, 471
BciT130I CCWGG 1 cut(s) 862
BclI TGATCA 2 cut(s) 468, 513
BcnI CCSGG 1 cut(s) 142
BcoDI GTCTC 3 cut(s) 9, 15, 425
BfaI CTAG 2 cut(s) 6, 177
BfoI RGCGCY 2 cut(s) 12, 138
BfuAI ACCTGC 1 cut(s) 286
BglI GCCNNNNNGGC 1 cut(s) 365
BisI GCNGC 9 cut(s) 187, 190, 305, 321, 338, 347, 357, 704, 885
BlsI GCNGC 9 cut(s) 188, 191, 306, 322, 339, 348, 358, 705, 886
Bme1390I CCNGG 2 cut(s) 142, 862
Bme18I GGWCC 1 cut(s) 129
BmeT110I CYCGRG 1 cut(s) 12
BmgBI CACGTC 1 cut(s) 60
BmgT120I GGNCC 2 cut(s) 129, 503
BmiI GGNNCC 3 cut(s) 101, 130, 797
BmrFI CCNGG 2 cut(s) 142, 862
BmtI GCTAGC 1 cut(s) 9
BpiI GAAGAC 1 cut(s) 57
BpmI CTGGAG 2 cut(s) 660, 782
BpuEI CTTGAG 3 cut(s) 719, 768, 821
BpuMI CCSGG 1 cut(s) 142
BsaHI GRCGYC 2 cut(s) 47, 195
BsaI GGTCTC 1 cut(s) 9
BsaJI CCNNGG 2 cut(s) 308, 341
BsaWI WCCGGW 1 cut(s) 225
BsaXI ACNNNNNCTCC 4 cut(s) 711, 741, 780, 810
Bsc4I CCNNNNNNNGG 4 cut(s) 95, 126, 157, 204
Bse118I RCCGGY 1 cut(s) 242
Bse1I ACTGG 3 cut(s) 655, 662, 737
BseAI TCCGGA 1 cut(s) 225
BseBI CCWGG 1 cut(s) 862
BseDI CCNNGG 2 cut(s) 308, 341
BseGI GGATG 1 cut(s) 53
BseLI CCNNNNNNNGG 4 cut(s) 95, 126, 157, 204
BseMII CTCAG 1 cut(s) 420
BseNI ACTGG 3 cut(s) 655, 662, 737
BseRI GAGGAG 2 cut(s) 385, 732
BseSI GKGCMC 1 cut(s) 420
BseX3I CGGCCG 1 cut(s) 357
BseXI GCAGC 5 cut(s) 201, 324, 333, 715, 871
Bsh1236I CGCG 2 cut(s) 182, 356
Bsh1285I CGRYCG 1 cut(s) 360
BshFI GGCC 5 cut(s) 304, 359, 505, 574, 654
BsiEI CGRYCG 1 cut(s) 360
BsiHKCI CYCGRG 1 cut(s) 12
BsiSI CCGG 3 cut(s) 141, 226, 243
BslFI GGGAC 1 cut(s) 142
BslI CCNNNNNNNGG 4 cut(s) 95, 126, 157, 204
BsmAI GTCTC 3 cut(s) 9, 15, 425
BsmBI CGTCTC 1 cut(s) 15
BsmFI GGGAC 1 cut(s) 142
BsnI GGCC 5 cut(s) 304, 359, 505, 574, 654
Bso31I GGTCTC 1 cut(s) 9
BsoBI CYCGRG 1 cut(s) 12
Bsp1286I GDGCHC 1 cut(s) 420
Bsp13I TCCGGA 1 cut(s) 225
Bsp143I GATC 5 cut(s) 331, 468, 481, 513, 626
BspACI CCGC 7 cut(s) 89, 186, 293, 305, 320, 323, 356
BspANI GGCC 5 cut(s) 304, 359, 505, 574, 654
BspCNI CTCAG 1 cut(s) 421
BspEI TCCGGA 1 cut(s) 225
BspFNI CGCG 2 cut(s) 182, 356
BspLI GGNNCC 3 cut(s) 101, 130, 797
BspMI ACCTGC 1 cut(s) 286
BspOI GCTAGC 1 cut(s) 9
BspPI GGATC 2 cut(s) 489, 634
BspQI GCTCTTC 1 cut(s) 882
BspTNI GGTCTC 1 cut(s) 9
BsrBI CCGCTC 2 cut(s) 91, 186
BsrFI RCCGGY 1 cut(s) 242
BsrI ACTGG 3 cut(s) 655, 662, 737
BssAI RCCGGY 1 cut(s) 242
BssECI CCNNGG 2 cut(s) 308, 341
BssMI GATC 5 cut(s) 331, 468, 481, 513, 626
BssNI GRCGYC 2 cut(s) 47, 195
Bst2UI CCWGG 1 cut(s) 862
Bst4CI ACNGT 1 cut(s) 250
Bst6I CTCTTC 2 cut(s) 525, 882
BstACI GRCGYC 2 cut(s) 47, 195
BstC8I GCNNGC 3 cut(s) 7, 184, 244
BstDEI CTNAG 2 cut(s) 429, 791
BstF5I GGATG 1 cut(s) 53
BstFNI CGCG 2 cut(s) 182, 356
BstH2I RGCGCY 2 cut(s) 12, 138
BstHHI GCGC 3 cut(s) 11, 137, 182
BstKTI GATC 5 cut(s) 334, 471, 484, 516, 629
BstMAI GTCTC 3 cut(s) 9, 15, 425
BstMBI GATC 5 cut(s) 331, 468, 481, 513, 626
BstMCI CGRYCG 1 cut(s) 360
BstMWI GCNNNNNNNGC 7 cut(s) 186, 301, 343, 346, 365, 703, 881
BstNI CCWGG 1 cut(s) 862
BstNSI RCATGY 1 cut(s) 272
BstSCI CCNGG 2 cut(s) 140, 860
BstSLI GKGCMC 1 cut(s) 420
BstUI CGCG 2 cut(s) 182, 356
BstV1I GCAGC 5 cut(s) 201, 324, 333, 715, 871
BstV2I GAAGAC 1 cut(s) 57
BstXI CCANNNNNNTGG 2 cut(s) 662, 806
BstZI CGGCCG 1 cut(s) 357
BsuRI GGCC 5 cut(s) 304, 359, 505, 574, 654
BtrI CACGTC 1 cut(s) 60
BtsCI GGATG 1 cut(s) 53
BtsIMutI CAGTG 2 cut(s) 648, 655
BveI ACCTGC 1 cut(s) 286
Cac8I GCNNGC 3 cut(s) 7, 184, 244
CfoI GCGC 3 cut(s) 11, 137, 182
Cfr10I RCCGGY 1 cut(s) 242
Cfr13I GGNCC 2 cut(s) 129, 503
CseI GACGC 2 cut(s) 55, 203
Csp6I GTAC 1 cut(s) 686
CviAII CATG 4 cut(s) 269, 466, 545, 576
CviQI GTAC 1 cut(s) 686
DdeI CTNAG 2 cut(s) 429, 791
DpnI GATC 5 cut(s) 333, 470, 483, 515, 628
DpnII GATC 5 cut(s) 331, 468, 481, 513, 626
EaeI YGGCCR 3 cut(s) 302, 357, 652
EagI CGGCCG 1 cut(s) 357
Eam1104I CTCTTC 2 cut(s) 525, 882
EarI CTCTTC 2 cut(s) 525, 882
EciI GGCGGA 1 cut(s) 338
EclXI CGGCCG 1 cut(s) 357
Eco31I GGTCTC 1 cut(s) 9
Eco32I GATATC 1 cut(s) 81
Eco47I GGWCC 1 cut(s) 129
Eco52I CGGCCG 1 cut(s) 357
Eco88I CYCGRG 1 cut(s) 12
EcoRI GAATTC 1 cut(s) 115
EcoRII CCWGG 1 cut(s) 860
EcoRV GATATC 1 cut(s) 81
Esp3I CGTCTC 1 cut(s) 15
FaeI CATG 4 cut(s) 272, 469, 548, 579
FalI AAGNNNNNCTT 2 cut(s) 864, 896
FaqI GGGAC 1 cut(s) 142
FatI CATG 4 cut(s) 268, 465, 544, 575
FauI CCCGC 2 cut(s) 96, 300
FbaI TGATCA 2 cut(s) 468, 513
FblI GTMKAC 2 cut(s) 550, 729
Fnu4HI GCNGC 9 cut(s) 187, 190, 305, 321, 338, 347, 357, 704, 885
FokI GGATG 1 cut(s) 40
Fsp4HI GCNGC 9 cut(s) 187, 190, 305, 321, 338, 347, 357, 704, 885
FspBI CTAG 2 cut(s) 6, 177
GlaI GCGC 3 cut(s) 10, 136, 181
GluI GCNGC 9 cut(s) 187, 190, 305, 321, 338, 347, 357, 704, 885
GsuI CTGGAG 2 cut(s) 660, 782
HaeII RGCGCY 2 cut(s) 12, 138
HaeIII GGCC 5 cut(s) 304, 359, 505, 574, 654
HapII CCGG 3 cut(s) 141, 226, 243
HgaI GACGC 2 cut(s) 55, 203
HhaI GCGC 3 cut(s) 11, 137, 182
Hin1I GRCGYC 2 cut(s) 47, 195
Hin1II CATG 4 cut(s) 272, 469, 548, 579
Hin6I GCGC 3 cut(s) 9, 135, 180
HinP1I GCGC 3 cut(s) 9, 135, 180
HincII GTYRAC 1 cut(s) 236
HindII GTYRAC 1 cut(s) 236
HinfI GANTC 6 cut(s) 26, 232, 394, 495, 522, 812
HpaII CCGG 3 cut(s) 141, 226, 243
HphI GGTGA 1 cut(s) 455
Hpy166II GTNNAC 4 cut(s) 236, 551, 581, 730
Hpy188I TCNGA 6 cut(s) 85, 109, 264, 473, 527, 763
Hpy188III TCNNGA 5 cut(s) 127, 151, 226, 677, 785
Hpy8I GTNNAC 4 cut(s) 236, 551, 581, 730
Hpy99I CGWCG 4 cut(s) 49, 64, 98, 197
HpyAV CCTTC 1 cut(s) 407
HpyCH4III ACNGT 1 cut(s) 250
HpyCH4IV ACGT 1 cut(s) 59
HpyCH4V TGCA 2 cut(s) 544, 605
HpyF10VI GCNNNNNNNGC 7 cut(s) 186, 301, 343, 346, 365, 703, 881
HpyF3I CTNAG 2 cut(s) 429, 791
HpySE526I ACGT 1 cut(s) 59
Hsp92I GRCGYC 2 cut(s) 47, 195
Hsp92II CATG 4 cut(s) 272, 469, 548, 579
HspAI GCGC 3 cut(s) 9, 135, 180
Kpn2I TCCGGA 1 cut(s) 225
KroI GCCGGC 1 cut(s) 242
KroNI GCCGGC 1 cut(s) 244
Ksp22I TGATCA 2 cut(s) 468, 513
Kzo9I GATC 5 cut(s) 331, 468, 481, 513, 626
LguI GCTCTTC 1 cut(s) 882
LmnI GCTCC 5 cut(s) 39, 144, 354, 679, 801
Lsp1109I GCAGC 5 cut(s) 201, 324, 333, 715, 871
MaeI CTAG 2 cut(s) 6, 177
MaeII ACGT 1 cut(s) 59
MaeIII GTNAC 1 cut(s) 646
MalI GATC 5 cut(s) 333, 470, 483, 515, 628
MbiI CCGCTC 2 cut(s) 91, 186
MboI GATC 5 cut(s) 331, 468, 481, 513, 626
MboII GAAGA 6 cut(s) 57, 542, 692, 776, 787, 869
MfeI CAATTG 1 cut(s) 692
MhlI GDGCHC 1 cut(s) 420
MlsI TGGCCA 1 cut(s) 654
MluCI AATT 6 cut(s) 115, 453, 615, 692, 723, 845
MluNI TGGCCA 1 cut(s) 654
MlyI GAGTC 5 cut(s) 20, 241, 388, 504, 821
MmeI TCCRAC 2 cut(s) 108, 132
Mox20I TGGCCA 1 cut(s) 654
MroI TCCGGA 1 cut(s) 225
MroNI GCCGGC 1 cut(s) 242
MroXI GAANNNNTTC 1 cut(s) 876
MscI TGGCCA 1 cut(s) 654
MseI TTAA 3 cut(s) 401, 452, 901
MslI CAYNNNNRTG 2 cut(s) 600, 660
Msp20I TGGCCA 1 cut(s) 654
MspI CCGG 3 cut(s) 141, 226, 243
MspR9I CCNGG 2 cut(s) 142, 862
MunI CAATTG 1 cut(s) 692
MvaI CCWGG 1 cut(s) 862
MvnI CGCG 2 cut(s) 182, 356
MwoI GCNNNNNNNGC 7 cut(s) 186, 301, 343, 346, 365, 703, 881
NaeI GCCGGC 1 cut(s) 244
NciI CCSGG 1 cut(s) 142
NdeII GATC 5 cut(s) 331, 468, 481, 513, 626
NgoMIV GCCGGC 1 cut(s) 242
NheI GCTAGC 1 cut(s) 5
NlaIII CATG 4 cut(s) 272, 469, 548, 579
NlaIV GGNNCC 3 cut(s) 101, 130, 797
NmeAIII GCCGAG 3 cut(s) 223, 333, 366
NmuCI GTSAC 1 cut(s) 646
NspI RCATGY 1 cut(s) 272
PaqCI CACCTGC 1 cut(s) 286
PciSI GCTCTTC 1 cut(s) 882
PcsI WCGNNNNNNNCGW 1 cut(s) 68
PdiI GCCGGC 1 cut(s) 244
PdmI GAANNNNTTC 1 cut(s) 876
PfeI GAWTC 1 cut(s) 522
PfoI TCCNGGA 1 cut(s) 140
PkrI GCNGC 9 cut(s) 188, 191, 306, 322, 339, 348, 358, 705, 886
PleI GAGTC 5 cut(s) 20, 240, 388, 503, 820
PpsI GAGTC 5 cut(s) 20, 240, 388, 503, 820
PshBI ATTAAT 1 cut(s) 452
Psp6I CCWGG 1 cut(s) 860
PspGI CCWGG 1 cut(s) 860
PspN4I GGNNCC 3 cut(s) 101, 130, 797
PspPI GGNCC 2 cut(s) 129, 503
RsaI GTAC 1 cut(s) 687
RsaNI GTAC 1 cut(s) 686
RseI CAYNNNNRTG 2 cut(s) 600, 660
SapI GCTCTTC 1 cut(s) 882
SaqAI TTAA 3 cut(s) 401, 452, 901
SatI GCNGC 9 cut(s) 187, 190, 305, 321, 338, 347, 357, 704, 885
Sau3AI GATC 5 cut(s) 331, 468, 481, 513, 626
Sau96I GGNCC 2 cut(s) 129, 503
SchI GAGTC 5 cut(s) 20, 241, 388, 504, 821
ScrFI CCNGG 2 cut(s) 142, 862
SduI GDGCHC 1 cut(s) 420
SetI ASST 6 cut(s) 62, 149, 300, 559, 691, 877
SinI GGWCC 1 cut(s) 129
SmiMI CAYNNNNRTG 2 cut(s) 600, 660
SmlI CTYRAG 3 cut(s) 698, 783, 836
SmoI CTYRAG 3 cut(s) 698, 783, 836
Sse9I AATT 6 cut(s) 115, 453, 615, 692, 723, 845
SsiI CCGC 7 cut(s) 89, 186, 293, 305, 320, 323, 356
SspMI CTAG 2 cut(s) 6, 177
StyD4I CCNGG 2 cut(s) 140, 860
TaaI ACNGT 1 cut(s) 250
TaiI ACGT 1 cut(s) 62
TaqI TCGA 2 cut(s) 93, 212
TaqII GACCGA 1 cut(s) 33
TasI AATT 6 cut(s) 115, 453, 615, 692, 723, 845
TauI GCSGC 4 cut(s) 189, 307, 323, 359
TfiI GAWTC 1 cut(s) 522
Tru1I TTAA 3 cut(s) 401, 452, 901
Tru9I TTAA 3 cut(s) 401, 452, 901
TscAI CASTG 2 cut(s) 655, 662
TseFI GTSAC 1 cut(s) 646
TseI GCWGC 5 cut(s) 189, 337, 346, 703, 884
Tsp45I GTSAC 1 cut(s) 646
TspGWI ACGGA 1 cut(s) 884
TspRI CASTG 2 cut(s) 655, 662
VpaK11BI GGWCC 1 cut(s) 129
VspI ATTAAT 1 cut(s) 452
XapI RAATTY 1 cut(s) 115
XceI RCATGY 1 cut(s) 272
XmiI GTMKAC 2 cut(s) 550, 729
XmnI GAANNNNTTC 1 cut(s) 876
XspI CTAG 2 cut(s) 6, 177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.