Rmu_ssc0000286.1_g000019

Protein EI24 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000286.1
OG0008616
Physical Location & Seq
Forward (+)
120573 .. 132042
11470 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1176 bp
atggaagctctgagcgcaaaatctaagctagccgcggtgctttggcttgagggcttcagacaggcctgttgccttcaccgtgtgctgattctctgccacaggtccagaaagcttctgatacgcacaggacagtgttttctgttgaacggtttcattttcttaggaagcataattatccttaactcgttcatcatcccagctctatactggatattgcctgatatgtgtccagaatctagttctcaagaattctgctcatttgctgtcatttgggaattttattccttcttacgtcatggactcatacaaatcttttatgtaacttggttatacccattatatgtattcagctttatcctgagcaacatctggtacaatgatattgccaagtatggattttctgcaatggggagacctgtgattaacaaattggatcccgtcaggcaaagtgatgcatcaaccttacaaaatgcagctggcatggaaagaccaactgacctaggacgggtcatgattggaataggagagcaggtttattcagtgcttcttttgagctgtttcttcttagaggtatatgtgacaggatttataccgtatgtgggcaaggcattaaattttctgcttctttcctggatgtatgcctactactgttttgagtacaaatggaatttttctgaaatcggtctagacaaaaggctggacttctttgaatctaactgggcattttttgctggttttggaagcccatgcgttgtacctcttttctttttctcacctcttgtgagctatgggtgtatggctatactctttccgctgtttgttttgacagctacaggttcagaagctgagcaacttatttcttctcgaagaacaagctggggaggcgcatgtttcggacggcttcaaatatttaagactgcagatagtttgtcgatgtggttgttgtcgctatttcctgtacaatcaaagagacgtctgcaagaacagaaatatctcagtcttcgacaacgaaactctccaaatcaattcgtctccttgctctggtcgtccctctggcatcctctagcggcgattctcacccacgacggcgctagaaggcggcgaagattcgtgtattcagacccgatcggttctcctatctccgacgtcggcgaggcttcaagtcttcttggttga

Protein Analysis

391

Amino Acids

44.72

Weight (kDa)

8.91

Isoelectric Point (pI)

57.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EI24 PF07264 24 - 271 1.3e-27 Sulfate transporter CysZ/Etoposide-induced protein 2.4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 976, 1149
Acc36I ACCTGC 1 cut(s) 520
AccII CGCG 1 cut(s) 35
AciI CCGC 5 cut(s) 33, 35, 812, 1067, 1099
AclWI GGATC 2 cut(s) 428, 441
AcsI RAATTY 4 cut(s) 248, 275, 613, 667
AcuI CTGAAG 1 cut(s) 40
AcyI GRCGYC 2 cut(s) 973, 1146
AdeI CACNNNGTG 1 cut(s) 82
AfaI GTAC 4 cut(s) 374, 659, 756, 960
AfiI CCNNNNNNNGG 3 cut(s) 505, 599, 1041
AgsI TTSAA 4 cut(s) 145, 710, 905, 1161
AjnI CCWGG 1 cut(s) 629
Alw26I GTCTC 3 cut(s) 406, 964, 1036
AlwI GGATC 2 cut(s) 428, 441
AlwNI CAGNNNCTG 1 cut(s) 845
AoxI GGCC 1 cut(s) 63
ApeKI GCWGC 1 cut(s) 473
ApoI RAATTY 4 cut(s) 248, 275, 613, 667
Asp700I GAANNNNTTC 1 cut(s) 149
AspA2I CCTAGG 1 cut(s) 499
AspLEI GCGC 3 cut(s) 17, 887, 1091
AspS9I GGNCC 1 cut(s) 102
AsuHPI GGTGA 3 cut(s) 68, 765, 1069
AsuNHI GCTAGC 1 cut(s) 28
AvaII GGWCC 1 cut(s) 102
AvrII CCTAGG 1 cut(s) 499
BamHI GGATCC 1 cut(s) 433
BbsI GAAGAC 2 cut(s) 992, 1157
BbvI GCAGC 1 cut(s) 485
BceAI ACGGC 2 cut(s) 914, 1102
BciT130I CCWGG 1 cut(s) 631
BcoDI GTCTC 3 cut(s) 406, 964, 1036
BfaI CTAG 6 cut(s) 29, 237, 500, 686, 1064, 1092
BfmI CTRYAG 2 cut(s) 831, 918
BfoI RGCGCY 1 cut(s) 1092
BfuAI ACCTGC 1 cut(s) 520
BisI GCNGC 4 cut(s) 33, 474, 1068, 1100
BlnI CCTAGG 1 cut(s) 499
BlpI GCTNAGC 1 cut(s) 846
BlsI GCNGC 4 cut(s) 34, 475, 1069, 1101
Bme1390I CCNGG 1 cut(s) 631
Bme18I GGWCC 1 cut(s) 102
BmgT120I GGNCC 1 cut(s) 102
BmiI GGNNCC 1 cut(s) 435
BmrFI CCNGG 1 cut(s) 631
BmrI ACTGGG 1 cut(s) 727
BmsI GCATC 3 cut(s) 442, 464, 1066
BmtI GCTAGC 1 cut(s) 32
BmuI ACTGGG 1 cut(s) 727
BpiI GAAGAC 2 cut(s) 992, 1157
Bpu10I CCTNAGC 1 cut(s) 359
Bpu1102I GCTNAGC 1 cut(s) 846
BpuEI CTTGAG 2 cut(s) 68, 228
BsaHI GRCGYC 2 cut(s) 973, 1146
BsaI GGTCTC 1 cut(s) 406
BsaJI CCNNGG 2 cut(s) 33, 499
BsaXI ACNNNNNCTCC 2 cut(s) 516, 546
Bsc4I CCNNNNNNNGG 3 cut(s) 505, 599, 1041
Bse1I ACTGG 2 cut(s) 212, 722
Bse3DI GCAATG 1 cut(s) 411
BseBI CCWGG 1 cut(s) 631
BseDI CCNNGG 2 cut(s) 33, 499
BseGI GGATG 3 cut(s) 192, 639, 1057
BseLI CCNNNNNNNGG 3 cut(s) 505, 599, 1041
BseMI GCAATG 1 cut(s) 411
BseMII CTCAG 3 cut(s) 350, 837, 1009
BseNI ACTGG 2 cut(s) 212, 722
BseXI GCAGC 1 cut(s) 485
BseYI CCCAGC 2 cut(s) 196, 876
Bsh1236I CGCG 1 cut(s) 35
Bsh1285I CGRYCG 1 cut(s) 1128
BshFI GGCC 1 cut(s) 65
BsiEI CGRYCG 1 cut(s) 1128
BslFI GGGAC 1 cut(s) 1033
BslI CCNNNNNNNGG 3 cut(s) 505, 599, 1041
BsmAI GTCTC 3 cut(s) 406, 964, 1036
BsmBI CGTCTC 2 cut(s) 964, 1036
BsmFI GGGAC 1 cut(s) 1033
BsnI GGCC 1 cut(s) 65
Bso31I GGTCTC 1 cut(s) 406
Bsp1407I TGTACA 1 cut(s) 958
Bsp143I GATC 2 cut(s) 433, 1125
Bsp1720I GCTNAGC 1 cut(s) 846
BspACI CCGC 5 cut(s) 33, 35, 812, 1067, 1099
BspANI GGCC 1 cut(s) 65
BspCNI CTCAG 3 cut(s) 351, 838, 1008
BspFNI CGCG 1 cut(s) 35
BspHI TCATGA 1 cut(s) 510
BspLI GGNNCC 1 cut(s) 435
BspMAI CTGCAG 1 cut(s) 922
BspMI ACCTGC 1 cut(s) 520
BspOI GCTAGC 1 cut(s) 32
BspPI GGATC 2 cut(s) 428, 441
BspTNI GGTCTC 1 cut(s) 406
BsrDI GCAATG 1 cut(s) 411
BsrGI TGTACA 1 cut(s) 958
BsrI ACTGG 2 cut(s) 212, 722
BssECI CCNNGG 2 cut(s) 33, 499
BssMI GATC 2 cut(s) 433, 1125
BssNI GRCGYC 2 cut(s) 973, 1146
BssT1I CCWWGG 1 cut(s) 499
Bst2UI CCWGG 1 cut(s) 631
Bst4CI ACNGT 5 cut(s) 80, 132, 149, 594, 650
BstACI GRCGYC 2 cut(s) 973, 1146
BstAPI GCANNNNNTGC 1 cut(s) 728
BstAUI TGTACA 1 cut(s) 958
BstC8I GCNNGC 2 cut(s) 30, 478
BstDEI CTNAG 7 cut(s) 11, 24, 160, 359, 565, 846, 995
BstDSI CCRYGG 1 cut(s) 33
BstF5I GGATG 3 cut(s) 192, 639, 1057
BstFNI CGCG 1 cut(s) 35
BstH2I RGCGCY 1 cut(s) 1092
BstHHI GCGC 3 cut(s) 17, 887, 1091
BstKTI GATC 2 cut(s) 436, 1128
BstMAI GTCTC 3 cut(s) 406, 964, 1036
BstMBI GATC 2 cut(s) 433, 1125
BstMCI CGRYCG 1 cut(s) 1128
BstMWI GCNNNNNNNGC 3 cut(s) 14, 728, 882
BstNI CCWGG 1 cut(s) 631
BstNSI RCATGY 1 cut(s) 891
BstSCI CCNGG 1 cut(s) 629
BstSFI CTRYAG 2 cut(s) 831, 918
BstUI CGCG 1 cut(s) 35
BstV1I GCAGC 1 cut(s) 485
BstV2I GAAGAC 2 cut(s) 992, 1157
BstX2I RGATCY 1 cut(s) 433
BstYI RGATCY 1 cut(s) 433
BsuRI GGCC 1 cut(s) 65
BtgI CCRYGG 1 cut(s) 33
BtsCI GGATG 3 cut(s) 192, 639, 1057
BtsIMutI CAGTG 2 cut(s) 137, 546
BveI ACCTGC 1 cut(s) 520
Cac8I GCNNGC 2 cut(s) 30, 478
CaiI CAGNNNCTG 1 cut(s) 845
CciI TCATGA 1 cut(s) 510
CfoI GCGC 3 cut(s) 17, 887, 1091
Cfr13I GGNCC 1 cut(s) 102
Cfr42I CCGCGG 1 cut(s) 36
Csp6I GTAC 4 cut(s) 373, 658, 755, 959
CviAII CATG 5 cut(s) 296, 481, 511, 747, 888
CviQI GTAC 4 cut(s) 373, 658, 755, 959
DdeI CTNAG 7 cut(s) 11, 24, 160, 359, 565, 846, 995
DpnI GATC 2 cut(s) 435, 1127
DpnII GATC 2 cut(s) 433, 1125
DraIII CACNNNGTG 1 cut(s) 82
Eco130I CCWWGG 1 cut(s) 499
Eco147I AGGCCT 1 cut(s) 65
Eco31I GGTCTC 1 cut(s) 406
Eco47I GGWCC 1 cut(s) 102
Eco57I CTGAAG 1 cut(s) 40
EcoRI GAATTC 1 cut(s) 248
EcoRII CCWGG 1 cut(s) 629
EcoT14I CCWWGG 1 cut(s) 499
EcoT22I ATGCAT 1 cut(s) 457
ErhI CCWWGG 1 cut(s) 499
Esp3I CGTCTC 2 cut(s) 964, 1036
FaeI CATG 5 cut(s) 299, 484, 514, 750, 891
FaqI GGGAC 1 cut(s) 1033
FatI CATG 5 cut(s) 295, 480, 510, 746, 887
Fnu4HI GCNGC 4 cut(s) 33, 474, 1068, 1100
FokI GGATG 3 cut(s) 179, 646, 1044
Fsp4HI GCNGC 4 cut(s) 33, 474, 1068, 1100
FspBI CTAG 6 cut(s) 29, 237, 500, 686, 1064, 1092
GlaI GCGC 3 cut(s) 16, 886, 1090
GluI GCNGC 4 cut(s) 33, 474, 1068, 1100
GsaI CCCAGC 2 cut(s) 200, 880
HaeII RGCGCY 1 cut(s) 1092
HaeIII GGCC 1 cut(s) 65
HhaI GCGC 3 cut(s) 17, 887, 1091
Hin1I GRCGYC 2 cut(s) 973, 1146
Hin1II CATG 5 cut(s) 299, 484, 514, 750, 891
Hin6I GCGC 3 cut(s) 15, 885, 1089
HinP1I GCGC 3 cut(s) 15, 885, 1089
HindIII AAGCTT 1 cut(s) 110
HinfI GANTC 6 cut(s) 88, 233, 300, 710, 1072, 1107
HphI GGTGA 3 cut(s) 68, 765, 1069
Hpy188I TCNGA 8 cut(s) 12, 59, 117, 676, 841, 896, 1120, 1144
Hpy188III TCNNGA 7 cut(s) 105, 230, 245, 358, 511, 686, 864
Hpy99I CGWCG 3 cut(s) 1088, 1148, 1151
HpyAV CCTTC 3 cut(s) 83, 295, 1089
HpyCH4III ACNGT 5 cut(s) 80, 132, 149, 594, 650
HpyCH4IV ACGT 3 cut(s) 292, 973, 1146
HpyCH4V TGCA 5 cut(s) 404, 455, 473, 920, 979
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 728, 882
HpyF3I CTNAG 7 cut(s) 11, 24, 160, 359, 565, 846, 995
HpySE526I ACGT 3 cut(s) 292, 973, 1146
Hsp92I GRCGYC 2 cut(s) 973, 1146
Hsp92II CATG 5 cut(s) 299, 484, 514, 750, 891
HspAI GCGC 3 cut(s) 15, 885, 1089
KspI CCGCGG 1 cut(s) 36
Kzo9I GATC 2 cut(s) 433, 1125
Lsp1109I GCAGC 1 cut(s) 485
LweI GCATC 3 cut(s) 442, 464, 1066
MaeI CTAG 6 cut(s) 29, 237, 500, 686, 1064, 1092
MaeII ACGT 3 cut(s) 292, 973, 1146
MaeIII GTNAC 2 cut(s) 319, 577
MalI GATC 2 cut(s) 435, 1127
MboI GATC 2 cut(s) 433, 1125
MboII GAAGA 6 cut(s) 553, 852, 879, 992, 1116, 1157
MflI RGATCY 1 cut(s) 433
MluCI AATT 7 cut(s) 171, 248, 275, 428, 613, 667, 1025
MlyI GAGTC 1 cut(s) 294
MmeI TCCRAC 1 cut(s) 1167
MnlI CCTC 8 cut(s) 43, 562, 768, 786, 875, 1061, 1071, 1147
Mph1103I ATGCAT 1 cut(s) 457
MroXI GAANNNNTTC 1 cut(s) 149
MseI TTAA 4 cut(s) 180, 423, 611, 912
MspA1I CMGCKG 3 cut(s) 35, 476, 814
MspR9I CCNGG 1 cut(s) 631
MvaI CCWGG 1 cut(s) 631
MvnI CGCG 1 cut(s) 35
MwoI GCNNNNNNNGC 3 cut(s) 14, 728, 882
NdeII GATC 2 cut(s) 433, 1125
NheI GCTAGC 1 cut(s) 28
NlaIII CATG 5 cut(s) 299, 484, 514, 750, 891
NlaIV GGNNCC 1 cut(s) 435
NmuCI GTSAC 1 cut(s) 577
NsiI ATGCAT 1 cut(s) 457
NspI RCATGY 1 cut(s) 891
PagI TCATGA 1 cut(s) 510
PceI AGGCCT 1 cut(s) 65
PdmI GAANNNNTTC 1 cut(s) 149
PfeI GAWTC 5 cut(s) 88, 233, 710, 1072, 1107
PfoI TCCNGGA 1 cut(s) 629
PkrI GCNGC 4 cut(s) 34, 475, 1069, 1101
Ple19I CGATCG 1 cut(s) 1128
PleI GAGTC 1 cut(s) 294
PpsI GAGTC 1 cut(s) 294
Psp6I CCWGG 1 cut(s) 629
PspFI CCCAGC 2 cut(s) 196, 876
PspGI CCWGG 1 cut(s) 629
PspN4I GGNNCC 1 cut(s) 435
PspPI GGNCC 1 cut(s) 102
PstI CTGCAG 1 cut(s) 922
PstNI CAGNNNCTG 1 cut(s) 845
PsuI RGATCY 1 cut(s) 433
PvuI CGATCG 1 cut(s) 1128
PvuII CAGCTG 1 cut(s) 476
RsaI GTAC 4 cut(s) 374, 659, 756, 960
RsaNI GTAC 4 cut(s) 373, 658, 755, 959
SacII CCGCGG 1 cut(s) 36
SaqAI TTAA 4 cut(s) 180, 423, 611, 912
SatI GCNGC 4 cut(s) 33, 474, 1068, 1100
Sau3AI GATC 2 cut(s) 433, 1125
Sau96I GGNCC 1 cut(s) 102
SchI GAGTC 1 cut(s) 294
ScrFI CCNGG 1 cut(s) 631
SfaNI GCATC 3 cut(s) 442, 464, 1066
SfcI CTRYAG 2 cut(s) 831, 918
Sfr303I CCGCGG 1 cut(s) 36
SgrBI CCGCGG 1 cut(s) 36
SinI GGWCC 1 cut(s) 102
SmlI CTYRAG 2 cut(s) 47, 243
SmoI CTYRAG 2 cut(s) 47, 243
Sse9I AATT 7 cut(s) 171, 248, 275, 428, 613, 667, 1025
SseBI AGGCCT 1 cut(s) 65
SsiI CCGC 5 cut(s) 33, 35, 812, 1067, 1099
SspI AATATT 1 cut(s) 909
SspMI CTAG 6 cut(s) 29, 237, 500, 686, 1064, 1092
StuI AGGCCT 1 cut(s) 65
StyD4I CCNGG 1 cut(s) 629
StyI CCWWGG 1 cut(s) 499
TaaI ACNGT 5 cut(s) 80, 132, 149, 594, 650
TaiI ACGT 3 cut(s) 295, 976, 1149
TaqI TCGA 3 cut(s) 865, 932, 1003
TaqII GACCGA 1 cut(s) 671
TasI AATT 7 cut(s) 171, 248, 275, 428, 613, 667, 1025
TatI WGTACW 2 cut(s) 657, 958
TauI GCSGC 3 cut(s) 35, 1070, 1102
TfiI GAWTC 5 cut(s) 88, 233, 710, 1072, 1107
Tru1I TTAA 4 cut(s) 180, 423, 611, 912
Tru9I TTAA 4 cut(s) 180, 423, 611, 912
TscAI CASTG 2 cut(s) 137, 546
TseFI GTSAC 1 cut(s) 577
TseI GCWGC 1 cut(s) 473
Tsp45I GTSAC 1 cut(s) 577
TspDTI ATGAA 2 cut(s) 142, 178
TspRI CASTG 2 cut(s) 137, 546
VpaK11BI GGWCC 1 cut(s) 102
XapI RAATTY 4 cut(s) 248, 275, 613, 667
XbaI TCTAGA 1 cut(s) 685
XceI RCATGY 1 cut(s) 891
XcmI CCANNNNNNNNNTGG 1 cut(s) 204
XmaJI CCTAGG 1 cut(s) 499
XmnI GAANNNNTTC 1 cut(s) 149
XspI CTAG 6 cut(s) 29, 237, 500, 686, 1064, 1092
ZraI GACGTC 2 cut(s) 974, 1147
Zsp2I ATGCAT 1 cut(s) 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.