Rmu_ssc0000291.1_g000033

DNA polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000291.1
OG0010240
Physical Location & Seq
Reverse (-)
145488 .. 151371
5884 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3165 bp
atgaagggtcaggagcaaagggatcgtcttttgggtcgtttatttgcttatggtgctcttggacgatcaggaagactagctgaggagtggatttctgacagaaatactccgcacataaaggaattcactagtcttctcattactcttgcatcaaagaagagatacttgcaagagcctgctgtatcaattattttagactcgattgaaaagttgcccctagaagctttgttgactcatgttctagaagctccaggacttcatgaatggtttgaaggggctattgaaattggaaatcctgatgctttgcttttggctctgaaaattggagaaaaactttctgtggacagtgcaagatttgggaaacttctgccagacccatttgttccaaacaaactgttctctgctgatcatctatcttctcttgctaatagtttaaaggaatctaccttctgtcagccacgcattcacaatgtttggcctgttctagtaaatattctgttgccagatagagttttgcaggctgaagatgcactgactatttcaaattccttaaagaagcacaaaaagaatcgcaagtctagctcctctgatgaagatattgcaaagaatttccagtgcttctgcgaggtgattattgagggatcccttctcccatcatcccatgatcgcaaacacttggcctttgatgttctgcttcttctcctaccgaggttgcctgcttcttacataccaatttgtttgtcttacaaagttgttcagtgcatgattgatgtacttccaacaatggatgcttggctcaagaaaattgtgcaaaattttattaagacattatcagaatgggtcggggatgatgatgtcagaagggtttctgtcataatggctctacagaaacacagcaatggtagatttgattgtatcacacgaacaaaaacagttaaggatttgatggcagacttcagaacagaatcgggttgcatgctgtttattcagaacttgttgaacatgttcgtggatgaaagtcatgcttctgatgaaccttcagatcagagtataacaacagatgacaattctgagataggttctattgaagacaaggactctgttgcaatgggaaattctgattttttgaaaacatggatagtggaatccctcccctgtatattaaaaaatttaaagctagaaccagaagctaagtttagggttcaaaaagaaattttgaagtttctagctgttcagggtttgttcactgcatctcttggcactgaagtgacatcctttgaattacaagagaaatttaggtggccaaaagcagccacctcaagtgctctttgcaggatgtgtattgagcagctccagttactgttggcaaattctcagaaaggagaaggtccacgtggtttgccaaatcgactcgagtcaaatgatcttggctcatattttatgcggtttcttagcactttgtgcaacattccttcaatttctctctttcgacctttggataccgaggaagaaaacactttgaaaaaattgcaagcgatggaaacttcactttctaaagaggaaaggaattgtgggcttagcagtgaggcaaataagttgcatgcactaagatacttgctcatccagctgcttttgcaaatgctccttcgaccgaaagaattcttagtagcggtgtctgagcttattatatgctgtaaaaaggctttccccgtttttgatcttgttgattcctgtggagaagacaacttagatggtgatgatgctcctgcagtaatggatgttcttgtggatacattgctttcattgctgccacagtcatcagctcctatgcgaactgctattgagcaggtctttaaatacttctgtgttgacatcacggatgatggtctgctgcggatgttgagggtcattaggaagaatttgaaacctgctagacatcaggatgctgatagtgaagacattgacgatgacgaagatgaagattttcttaacattgaagaagatgaggtcattgatagagctgagactggtgaaacaggtgacagtgatgagcagacagacgagtctgaggccgactctgaggctgttgatggtgttggggaagttcccatggaaattcctgacgcttctgatgaatcagatggagggatggatgatgatgcaatgttccggatggacacttatcttgcacggatcttcaaagagcgaaagaatctagctgggggtgacactgctcatcagcaactcatgctgtttaagcttcgagttctttctttgctagaaatttacctgcatgaaaatccagataagccacaggttttgttggtttactcaaatttggctcgcgcgtttgctgaaccgcatactgcagaaagtagtgaacagcttggacagcgtatatggggaattctgcaaaagaaaatatttaaagcaaaggaccatcccaagggtgaagatgtggaactatctactctagaatctttgttgcaaagaaatttgaagttggcttcaaaaccaatcaagagaaagaaatctgctgccaacccgtcaaagaagaagcagtcggcctcctggaaccgacagaaaatgattgcttcccttgcacagagttctaccttctggattctgaagatcattgatgcaagaaattttccagagtctgagctgcagagagtttttgatattttccagggtgttctggtagaatatttcaatagtaagaagtcccaaatcaaatctgaatttttgaaagaaatatttagaagaaggccttggatggggcgttatctctttggcttccttttggagaaatgcggaagttcaaagtcagactttcggcgagtagaagcactcgatatggtgagtgaaatattgaagtccccgggcttatctgatggaagtggccaggaagctttgaagaaaattatgaaaagccatctgcaaaaactctgtcagttgatagagcaattgcttacaaatatgccagaaaagcagtcaagacgagctgaagtacggaagttttgcgggaagatctttcagatgatagcaacccttaagctgagcaagtcttttctcaagaatttagctccagatgctcacgccaagtgtgaatcacagcttggtgatcagtttaagaacttgaagaagatacaggttggcgtgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000182 GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003712 GO:0003714 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006091 GO:0006139 GO:0006351 GO:0006355 GO:0006360 GO:0006725 GO:0006807 GO:0006915 GO:0006950 GO:0007154 GO:0007165 GO:0007623 GO:0008134 GO:0008150 GO:0008152 GO:0008219 GO:0008270 GO:0009058 GO:0009059 GO:0009267 GO:0009303 GO:0009605 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0009991 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010629 GO:0010941 GO:0010942 GO:0012501 GO:0015980 GO:0016070 GO:0016072 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022900 GO:0022904 GO:0023052 GO:0031074 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032501 GO:0032774 GO:0032922 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0035556 GO:0042149 GO:0042564 GO:0042594 GO:0042790 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045333 GO:0045787 GO:0045892 GO:0045934 GO:0046483 GO:0046872 GO:0046914 GO:0048511 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0051726 GO:0055114 GO:0060255 GO:0065007 GO:0070013 GO:0070888 GO:0071156 GO:0071158 GO:0071496 GO:0071704 GO:0072331 GO:0072332 GO:0080090 GO:0090068 GO:0090304 GO:0097159 GO:0097190 GO:0097193 GO:0097659 GO:0098781 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000112 GO:2000113 GO:2000209 GO:2000210 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_phi

Protein Analysis

1054

Amino Acids

119.07

Weight (kDa)

5.69

Isoelectric Point (pI)

43.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_phi PF04931 1 - 764 1e-159 DNA polymerase phi
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010240)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64420
fragaria_vesca FvH4_2g11160
malus_domestica MD05G1049800.v1.1 MD10G1056900.v1.1
prunus_persica Prupe.8G075100_v2.0.a1
pyrus_communis pycom05g04160 pycom05g04170 pycom10g04380
rosa_chinensis RchiOBHm_Chr6g0269481
rosa_laevigata RLG00000013848 RLG00000013849
rosa_multiflora Rmu_ssc0000291.1_g000033
rosa_roxburghii Rroxscaffold_7G00198700
rosa_rugosa Rorug06G0048500
rosa_samantha Rh6AG168400 Rh6BG173800 Rh6CG168000 Rh6CG168100 Rh6DG160000 Rh6DG160100
rosa_wichuraiana Rw6G014500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2062
Acc36I ACCTGC 3 cut(s) 1837, 1936, 2298
AccII CGCG 2 cut(s) 2346, 2348
AccIII TCCGGA 1 cut(s) 2169
AciI CCGC 7 cut(s) 110, 1444, 1670, 1894, 2360, 2814, 3024
AclWI GGATC 4 cut(s) 30, 636, 649, 2201
AcoI YGGCCR 2 cut(s) 1301, 2902
AcuI CTGAAG 6 cut(s) 543, 940, 1023, 1284, 2648, 3027
AcvI CACGTG 1 cut(s) 1394
AdeI CACNNNGTG 1 cut(s) 1461
AfaI GTAC 2 cut(s) 774, 3012
AfiI CCNNNNNNNGG 2 cut(s) 2446, 2777
AflII CTTAAG 1 cut(s) 3053
AflIII ACRYGT 1 cut(s) 1002
AhlI ACTAGT 1 cut(s) 128
AjnI CCWGG 4 cut(s) 250, 2570, 2688, 2904
AjuI GAANNNNNNNTTGG 4 cut(s) 2755, 2787, 3102, 3134
AleI CACNNNNGTG 1 cut(s) 1265
Alw21I GWGCWC 2 cut(s) 58, 1327
Alw26I GTCTC 1 cut(s) 2018
AlwI GGATC 4 cut(s) 30, 636, 649, 2201
AlwNI CAGNNNCTG 2 cut(s) 1360, 2660
Ama87I CYCGRG 2 cut(s) 1412, 2881
Aor13HI TCCGGA 1 cut(s) 2169
AoxI GGCC 7 cut(s) 476, 678, 1301, 2070, 2565, 2768, 2902
ApeKI GCWGC 7 cut(s) 1310, 1348, 1627, 1807, 1891, 2537, 2665
ArsI GACNNNNNNTTYG 4 cut(s) 10, 42, 2935, 2967
Asp700I GAANNNNTTC 4 cut(s) 865, 1004, 1658, 1983
AspLEI GCGC 1 cut(s) 2348
AspS9I GGNCC 2 cut(s) 1388, 2437
AsuC2I CCSGG 2 cut(s) 2882, 2883
AsuHPI GGTGA 8 cut(s) 640, 1766, 2042, 2051, 2237, 2462, 2872, 3134
AvaI CYCGRG 2 cut(s) 1412, 2881
AvaII GGWCC 2 cut(s) 1388, 2437
BalI TGGCCA 2 cut(s) 1303, 2904
BamHI GGATCC 1 cut(s) 641
BbrPI CACGTG 1 cut(s) 1394
BbsI GAAGAC 5 cut(s) 79, 125, 1095, 1746, 1962
Bbv12I GWGCWC 2 cut(s) 58, 1327
BbvCI CCTCAGC 1 cut(s) 81
BbvI GCAGC 7 cut(s) 1322, 1360, 1614, 1794, 1878, 2524, 2652
BcgI CGANNNNNNTGC 2 cut(s) 5, 39
BciT130I CCWGG 4 cut(s) 252, 2572, 2690, 2906
BciVI GTATCC 2 cut(s) 1492, 1783
BclI TGATCA 2 cut(s) 406, 3124
BcnI CCSGG 2 cut(s) 2882, 2883
BcoDI GTCTC 1 cut(s) 2018
BcuI ACTAGT 1 cut(s) 128
BfmI CTRYAG 4 cut(s) 884, 1767, 2367, 2666
BfrI CTTAAG 1 cut(s) 3053
BfuAI ACCTGC 3 cut(s) 1837, 1936, 2298
BfuI GTATCC 2 cut(s) 1492, 1783
BglII AGATCT 1 cut(s) 3030
BisI GCNGC 7 cut(s) 1311, 1349, 1628, 1808, 1892, 2538, 2666
BlpI GCTNAGC 2 cut(s) 1577, 3059
BlsI GCNGC 7 cut(s) 1312, 1350, 1629, 1809, 1893, 2539, 2667
Bme1390I CCNGG 6 cut(s) 252, 2572, 2690, 2882, 2883, 2906
Bme18I GGWCC 2 cut(s) 1388, 2437
BmeT110I CYCGRG 2 cut(s) 1412, 2881
BmgT120I GGNCC 2 cut(s) 1388, 2437
BmiI GGNNCC 2 cut(s) 643, 2576
BmrFI CCNGG 6 cut(s) 252, 2572, 2690, 2882, 2883, 2906
BoxI GACNNNNGTC 1 cut(s) 1414
BpiI GAAGAC 5 cut(s) 79, 125, 1095, 1746, 1962
BplI GAGNNNNNCTC 2 cut(s) 2060, 2092
BpmI CTGGAG 3 cut(s) 234, 1337, 3072
Bpu10I CCTNAGC 1 cut(s) 81
Bpu1102I GCTNAGC 2 cut(s) 1577, 3059
BpuEI CTTGAG 3 cut(s) 782, 1303, 3059
BpuMI CCSGG 2 cut(s) 2882, 2883
BsaAI YACGTR 1 cut(s) 1394
BsaJI CCNNGG 8 cut(s) 707, 1503, 2109, 2445, 2689, 2771, 2880, 2881
BsaWI WCCGGW 1 cut(s) 2169
Bsc4I CCNNNNNNNGG 2 cut(s) 2446, 2777
Bse1I ACTGG 3 cut(s) 613, 1354, 2032
Bse3DI GCAATG 5 cut(s) 904, 1113, 1793, 1802, 2169
BseAI TCCGGA 1 cut(s) 2169
BseBI CCWGG 4 cut(s) 252, 2572, 2690, 2906
BseDI CCNNGG 8 cut(s) 707, 1503, 2109, 2445, 2689, 2771, 2880, 2881
BseLI CCNNNNNNNGG 2 cut(s) 2446, 2777
BseMI GCAATG 5 cut(s) 904, 1113, 1793, 1802, 2169
BseMII CTCAG 9 cut(s) 72, 1062, 1388, 1668, 2013, 2058, 2070, 2652, 3050
BseNI ACTGG 3 cut(s) 613, 1354, 2032
BseRI GAGGAG 2 cut(s) 98, 574
BseXI GCAGC 7 cut(s) 1322, 1360, 1614, 1794, 1878, 2524, 2652
BseYI CCCAGC 1 cut(s) 2219
Bsh1236I CGCG 2 cut(s) 2346, 2348
Bsh1285I CGRYCG 1 cut(s) 1652
BshFI GGCC 7 cut(s) 478, 680, 1303, 2072, 2567, 2770, 2904
BsiEI CGRYCG 1 cut(s) 1652
BsiHKAI GWGCWC 2 cut(s) 58, 1327
BsiHKCI CYCGRG 2 cut(s) 1412, 2881
BsiSI CCGG 2 cut(s) 2170, 2882
BslFI GGGAC 2 cut(s) 2710, 2863
BslI CCNNNNNNNGG 2 cut(s) 2446, 2777
BsmAI GTCTC 1 cut(s) 2018
BsmFI GGGAC 2 cut(s) 2710, 2863
BsmI GAATGC 1 cut(s) 462
BsnI GGCC 7 cut(s) 478, 680, 1303, 2072, 2567, 2770, 2904
BsoBI CYCGRG 2 cut(s) 1412, 2881
Bsp1286I GDGCHC 2 cut(s) 58, 1327
Bsp13I TCCGGA 1 cut(s) 2169
Bsp1720I GCTNAGC 2 cut(s) 1577, 3059
Bsp19I CCATGG 1 cut(s) 2109
BspACI CCGC 7 cut(s) 110, 1444, 1670, 1894, 2360, 2814, 3024
BspANI GGCC 7 cut(s) 478, 680, 1303, 2072, 2567, 2770, 2904
BspCNI CTCAG 9 cut(s) 73, 1063, 1387, 1669, 2014, 2059, 2071, 2653, 3051
BspEI TCCGGA 1 cut(s) 2169
BspFNI CGCG 2 cut(s) 2346, 2348
BspHI TCATGA 1 cut(s) 259
BspLI GGNNCC 2 cut(s) 643, 2576
BspMAI CTGCAG 3 cut(s) 1771, 2371, 2670
BspMI ACCTGC 3 cut(s) 1837, 1936, 2298
BspPI GGATC 4 cut(s) 30, 636, 649, 2201
BspTI CTTAAG 1 cut(s) 3053
BsrDI GCAATG 5 cut(s) 904, 1113, 1793, 1802, 2169
BsrI ACTGG 3 cut(s) 613, 1354, 2032
BssECI CCNNGG 8 cut(s) 707, 1503, 2109, 2445, 2689, 2771, 2880, 2881
BssT1I CCWWGG 3 cut(s) 2109, 2445, 2771
Bst2UI CCWGG 4 cut(s) 252, 2572, 2690, 2906
Bst4CI ACNGT 6 cut(s) 347, 396, 934, 1362, 1815, 2045
Bst6I CTCTTC 1 cut(s) 152
BstAFI CTTAAG 1 cut(s) 3053
BstAPI GCANNNNNTGC 2 cut(s) 1461, 2248
BstBAI YACGTR 1 cut(s) 1394
BstC8I GCNNGC 7 cut(s) 177, 519, 717, 977, 1533, 1602, 2344
BstDSI CCRYGG 1 cut(s) 2109
BstFNI CGCG 2 cut(s) 2346, 2348
BstHHI GCGC 1 cut(s) 2348
BstMAI GTCTC 1 cut(s) 2018
BstMCI CGRYCG 1 cut(s) 1652
BstNI CCWGG 4 cut(s) 252, 2572, 2690, 2906
BstNSI RCATGY 3 cut(s) 979, 1006, 1604
BstPAI GACNNNNGTC 1 cut(s) 1414
BstSCI CCNGG 6 cut(s) 250, 2570, 2688, 2880, 2881, 2904
BstSFI CTRYAG 4 cut(s) 884, 1767, 2367, 2666
BstUI CGCG 2 cut(s) 2346, 2348
BstV1I GCAGC 7 cut(s) 1322, 1360, 1614, 1794, 1878, 2524, 2652
BstV2I GAAGAC 5 cut(s) 79, 125, 1095, 1746, 1962
BstX2I RGATCY 3 cut(s) 641, 2193, 3030
BstYI RGATCY 3 cut(s) 641, 2193, 3030
BsuI GTATCC 2 cut(s) 1492, 1783
BsuRI GGCC 7 cut(s) 478, 680, 1303, 2072, 2567, 2770, 2904
BtgI CCRYGG 1 cut(s) 2109
BtgZI GCGATG 1 cut(s) 1550
BtsI GCAGTG 3 cut(s) 1245, 1588, 2229
BtsIMutI CAGTG 9 cut(s) 352, 530, 620, 764, 1245, 1260, 1588, 2050, 2229
BveI ACCTGC 3 cut(s) 1837, 1936, 2298
Cac8I GCNNGC 7 cut(s) 177, 519, 717, 977, 1533, 1602, 2344
CaiI CAGNNNCTG 2 cut(s) 1360, 2660
CciI TCATGA 1 cut(s) 259
CfoI GCGC 1 cut(s) 2348
Cfr13I GGNCC 2 cut(s) 1388, 2437
Cfr9I CCCGGG 1 cut(s) 2881
CseI GACGC 1 cut(s) 2132
Csp6I GTAC 2 cut(s) 773, 3011
CspCI CAANNNNNGTGG 4 cut(s) 1380, 1415, 2301, 2336
CviQI GTAC 2 cut(s) 773, 3011
DraI TTTAAA 4 cut(s) 435, 1173, 1855, 2428
DraIII CACNNNGTG 1 cut(s) 1461
DrdI GACNNNNNNGTC 1 cut(s) 2062
DseDI GACNNNNNNGTC 1 cut(s) 2062
EaeI YGGCCR 2 cut(s) 1301, 2902
Eam1104I CTCTTC 1 cut(s) 152
EarI CTCTTC 1 cut(s) 152
Eco130I CCWWGG 3 cut(s) 2109, 2445, 2771
Eco147I AGGCCT 1 cut(s) 2770
Eco47I GGWCC 2 cut(s) 1388, 2437
Eco57I CTGAAG 6 cut(s) 543, 940, 1023, 1284, 2648, 3027
Eco72I CACGTG 1 cut(s) 1394
Eco88I CYCGRG 2 cut(s) 1412, 2881
EcoRI GAATTC 3 cut(s) 122, 1658, 2406
EcoRII CCWGG 4 cut(s) 250, 2570, 2688, 2904
EcoT14I CCWWGG 3 cut(s) 2109, 2445, 2771
ErhI CCWWGG 3 cut(s) 2109, 2445, 2771
FaqI GGGAC 2 cut(s) 2710, 2863
FauI CCCGC 1 cut(s) 3017
FbaI TGATCA 2 cut(s) 406, 3124
Fnu4HI GCNGC 7 cut(s) 1311, 1349, 1628, 1808, 1892, 2538, 2666
Fsp4HI GCNGC 7 cut(s) 1311, 1349, 1628, 1808, 1892, 2538, 2666
GlaI GCGC 1 cut(s) 2347
GluI GCNGC 7 cut(s) 1311, 1349, 1628, 1808, 1892, 2538, 2666
GsaI CCCAGC 1 cut(s) 2223
GsuI CTGGAG 3 cut(s) 234, 1337, 3072
HaeIII GGCC 7 cut(s) 478, 680, 1303, 2072, 2567, 2770, 2904
HapII CCGG 2 cut(s) 2170, 2882
HgaI GACGC 1 cut(s) 2132
HhaI GCGC 1 cut(s) 2348
Hin6I GCGC 1 cut(s) 2346
HinP1I GCGC 1 cut(s) 2346
HincII GTYRAC 2 cut(s) 231, 1870
HindII GTYRAC 2 cut(s) 231, 1870
HindIII AAGCTT 3 cut(s) 222, 2258, 2910
HpaII CCGG 2 cut(s) 2170, 2882
HphI GGTGA 8 cut(s) 640, 1766, 2042, 2051, 2237, 2462, 2872, 3134
Hpy166II GTNNAC 7 cut(s) 231, 343, 1245, 1391, 1870, 2329, 2381
Hpy8I GTNNAC 7 cut(s) 231, 343, 1245, 1391, 1870, 2329, 2381
HpyCH4III ACNGT 6 cut(s) 347, 396, 934, 1362, 1815, 2045
HpyCH4IV ACGT 1 cut(s) 1393
HpySE526I ACGT 1 cut(s) 1393
HspAI GCGC 1 cut(s) 2346
Kpn2I TCCGGA 1 cut(s) 2169
Ksp22I TGATCA 2 cut(s) 406, 3124
LmnI GCTCC 8 cut(s) 13, 253, 587, 1356, 1647, 1768, 1828, 3091
Lsp1109I GCAGC 7 cut(s) 1322, 1360, 1614, 1794, 1878, 2524, 2652
MaeII ACGT 1 cut(s) 1393
MaeIII GTNAC 4 cut(s) 1267, 1356, 2039, 2225
MfeI CAATTG 1 cut(s) 2966
MflI RGATCY 3 cut(s) 641, 2193, 3030
MhlI GDGCHC 2 cut(s) 58, 1327
MlsI TGGCCA 2 cut(s) 1303, 2904
MluNI TGGCCA 2 cut(s) 1303, 2904
MlyI GAGTC 8 cut(s) 191, 226, 1091, 1404, 1424, 2069, 2072, 2666
MmeI TCCRAC 1 cut(s) 803
Mox20I TGGCCA 2 cut(s) 1303, 2904
MroI TCCGGA 1 cut(s) 2169
MroXI GAANNNNTTC 4 cut(s) 865, 1004, 1658, 1983
MscI TGGCCA 2 cut(s) 1303, 2904
MslI CAYNNNNRTG 4 cut(s) 897, 1007, 1265, 1941
Msp20I TGGCCA 2 cut(s) 1303, 2904
MspA1I CMGCKG 1 cut(s) 1627
MspCI CTTAAG 1 cut(s) 3053
MspI CCGG 2 cut(s) 2170, 2882
MspR9I CCNGG 6 cut(s) 252, 2572, 2690, 2882, 2883, 2906
MunI CAATTG 1 cut(s) 2966
Mva1269I GAATGC 1 cut(s) 462
MvaI CCWGG 4 cut(s) 252, 2572, 2690, 2906
MvnI CGCG 2 cut(s) 2346, 2348
NciI CCSGG 2 cut(s) 2882, 2883
NcoI CCATGG 1 cut(s) 2109
NlaIV GGNNCC 2 cut(s) 643, 2576
NmuCI GTSAC 3 cut(s) 1267, 2039, 2225
NspI RCATGY 3 cut(s) 979, 1006, 1604
OliI CACNNNNGTG 1 cut(s) 1265
PaeI GCATGC 2 cut(s) 979, 1604
PaeR7I CTCGAG 1 cut(s) 1412
PagI TCATGA 1 cut(s) 259
PceI AGGCCT 1 cut(s) 2770
PciI ACATGT 1 cut(s) 1002
PctI GAATGC 1 cut(s) 462
PdmI GAANNNNTTC 4 cut(s) 865, 1004, 1658, 1983
PfoI TCCNGGA 2 cut(s) 250, 2570
PkrI GCNGC 7 cut(s) 1312, 1350, 1629, 1809, 1893, 2539, 2667
PleI GAGTC 8 cut(s) 191, 226, 1091, 1404, 1423, 2069, 2071, 2665
PmaCI CACGTG 1 cut(s) 1394
PmlI CACGTG 1 cut(s) 1394
PpsI GAGTC 8 cut(s) 191, 226, 1091, 1404, 1423, 2069, 2071, 2665
Ppu21I YACGTR 1 cut(s) 1394
PscI ACATGT 1 cut(s) 1002
PshAI GACNNNNGTC 1 cut(s) 1414
Psp6I CCWGG 4 cut(s) 250, 2570, 2688, 2904
PspCI CACGTG 1 cut(s) 1394
PspFI CCCAGC 1 cut(s) 2219
PspGI CCWGG 4 cut(s) 250, 2570, 2688, 2904
PspN4I GGNNCC 2 cut(s) 643, 2576
PspPI GGNCC 2 cut(s) 1388, 2437
PspXI VCTCGAGB 1 cut(s) 1412
PsrI GAACNNNNNNTAC 2 cut(s) 1764, 1796
PstI CTGCAG 3 cut(s) 1771, 2371, 2670
PstNI CAGNNNCTG 2 cut(s) 1360, 2660
PsuI RGATCY 3 cut(s) 641, 2193, 3030
PvuII CAGCTG 1 cut(s) 1627
RsaI GTAC 2 cut(s) 774, 3012
RsaNI GTAC 2 cut(s) 773, 3011
RseI CAYNNNNRTG 4 cut(s) 897, 1007, 1265, 1941
SatI GCNGC 7 cut(s) 1311, 1349, 1628, 1808, 1892, 2538, 2666
Sau96I GGNCC 2 cut(s) 1388, 2437
SchI GAGTC 8 cut(s) 191, 226, 1091, 1404, 1424, 2069, 2072, 2666
ScrFI CCNGG 6 cut(s) 252, 2572, 2690, 2882, 2883, 2906
SduI GDGCHC 2 cut(s) 58, 1327
SfcI CTRYAG 4 cut(s) 884, 1767, 2367, 2666
Sfr274I CTCGAG 1 cut(s) 1412
SinI GGWCC 2 cut(s) 1388, 2437
SlaI CTCGAG 1 cut(s) 1412
SmaI CCCGGG 1 cut(s) 2883
SmiMI CAYNNNNRTG 4 cut(s) 897, 1007, 1265, 1941
SmlI CTYRAG 5 cut(s) 797, 1318, 1412, 3053, 3074
SmoI CTYRAG 5 cut(s) 797, 1318, 1412, 3053, 3074
SpeI ACTAGT 1 cut(s) 128
SphI GCATGC 2 cut(s) 979, 1604
SseBI AGGCCT 1 cut(s) 2770
SsiI CCGC 7 cut(s) 110, 1444, 1670, 1894, 2360, 2814, 3024
SspI AATATT 5 cut(s) 493, 2424, 2708, 2757, 2871
StuI AGGCCT 1 cut(s) 2770
StyD4I CCNGG 6 cut(s) 250, 2570, 2688, 2880, 2881, 2904
StyI CCWWGG 3 cut(s) 2109, 2445, 2771
TaaI ACNGT 6 cut(s) 347, 396, 934, 1362, 1815, 2045
TaiI ACGT 1 cut(s) 1396
TaqI TCGA 7 cut(s) 200, 1408, 1413, 1489, 1648, 2263, 2853
TaqII GACCGA 1 cut(s) 1666
TatI WGTACW 1 cut(s) 772
TscAI CASTG 9 cut(s) 352, 537, 620, 764, 1252, 1267, 1588, 2050, 2236
TseFI GTSAC 3 cut(s) 1267, 2039, 2225
TseI GCWGC 7 cut(s) 1310, 1348, 1627, 1807, 1891, 2537, 2665
Tsp45I GTSAC 3 cut(s) 1267, 2039, 2225
TspGWI ACGGA 3 cut(s) 1892, 2206, 3028
TspMI CCCGGG 1 cut(s) 2881
TspRI CASTG 9 cut(s) 352, 537, 620, 764, 1252, 1267, 1588, 2050, 2236
Vha464I CTTAAG 1 cut(s) 3053
VpaK11BI GGWCC 2 cut(s) 1388, 2437
XbaI TCTAGA 2 cut(s) 241, 2473
XceI RCATGY 3 cut(s) 979, 1006, 1604
XhoI CTCGAG 1 cut(s) 1412
XmaI CCCGGG 1 cut(s) 2881
XmnI GAANNNNTTC 4 cut(s) 865, 1004, 1658, 1983
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.