Rmu_ssc0000385.1_g000007

C2 and GRAM domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000385.1
OG0006300
Physical Location & Seq
Reverse (-)
19465 .. 24217
4753 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3081 bp
atgaagctggtggttcaagtgatgggggctcgagatctgccggcaatggatctaaacgggctgagtgatccgtacgtgaaggtgaagatgggaaagcagaagttcaagaccaaagtggtgaagaagacgttaaacccatattggggtgaagagttcacgtttcgagtcgacgacctcaacgacgagctcctcatctcttgtttggatgaagataagtacttcaacgacgacttcgtgggctgcgtcaaattccctgtttcccaagtcttcgatgcccccaacaagtgcctcgacacttgttggcaccctctccagcccaagagcaagaagcccaagcacaaggattgcggcgagattcttcttaatatctctctcagtacaaacaatgcattctccgactccgcttccgatggtgatcacatcaggagggattccgacattggaggtgaatcaccctcaaggttatccgaaacggcttcggcttcccctcagagaggcaagcttgatgaacttccattctccaaggaagataaagaaaaggaaaagactcttgcccagaaatcccttgctggccgacttgttcagatgttcaataaaaatcctgacgcacctccaatatcttcaaaccactctagcaaagctgacttgactgagcttgttgaaaccaaggccgacatttctgaggccacttattctgaggatcactcctcttgtgttccttttgaagagctaatgaaaacaatgcagttacgcgatcaggctactgaaacccccgagaacctaccgggtggagtgcttctagatcaaatgtatgttactgaacccaaacacctcaacactttactcttttcaccggattctacttttccaaaggcgttggcagacctgcatggaactacagaattggaacaaggaccctggaaatttgaaaacgacagcttaaaaagagtggttacatatgttaaggcagctagcaaattaatcaaggcttgcaaaggaagtgaggaccaaacatatctcaaagccgatgggaaagtttttgcagttttttcaagtgtaagcactcctgatgttccatatgggaaaacttttaggaccgagttgctctactgcattacacctgggcctgagctgccatcgggagaacaatctacacgctttgtaatatcctggcgcatgaattttctgcagagcaccatgatgaaaggaatgatagaaaatggagctcggcaaggtttaaaggacagttatgagcagtatgcaactttattatcgcagaacgttaagccagctgattccaaagaccttggatccaataaggaccaggttttggcatcattgcaggctgaatcccagtctgattggaagctggcagtacaatattttgctaatttcactgtggtctccacattttttattggattgtatatgctggtacacatctacctggctacacccagcactattcaagggttggagtttgttgggcttgacttgccagattcaattggtgaattcattgtttgtggtgtcctggtacttcaaggagaacgtgtactgggtttgatctcacgctttatgcaggccagagtgcagaaaggcagtgaccatggagtcaaagcccgaggagatggttggttgctaactgttgccttgattgaaggaagtaatatagcagctgtagattcaagtgggttctctgatccatacgtggtgttttcttgcaatggaaaagctagaactagctcaatcaagttccagaaatgcgaccctatgtggaacgaaatatttgagtttgatgcaatggatgagcctccttctgtgctggatgtggaaatttatgattttgatggtccttttgatgaagctacctctctgggccatgctgaaatcaattttgtgaaaaccaatatatcagatttagctgatatgtggattcctcttcaaggaaagttagctcaggcttgtcagtcaaaactccacttgagaatttttttaaacaatacaagaggtggcaatgttgttaatcactttttaaataagatggagaaggaggttggaaagaagattactgtacgttctcctcaaacaaattcagcattccaaaaactctttgggcttccaccagaggaatttcttatcaacgactttacatgtcacttgaaacgaaaaatgcccctgcagggccgcctatttctttctgcgagaataattgggttccatgcaaatttatttggtcgcaagacaaagttctttttcctctgggaggatatagaagatattcatattgttcctccaactctgtcatctatgggtagtccaactatagccatgactctgcggcaaggtagaggtatggatgcaaggcatggtgcaaagacacaggatgaggaaggcaggctgaagttccacttccagtccttcgtatccttcaatgtagcgaacaggacaattatggccctgtggaaggctagatctttgagtcctgagcagaaggtgcaaataatagaagaagaatcagaagtcaagatcctccaaactgaagagagtgggtctttcttgggccttgatgatgtcagcatgtctgaggtttattcatccttgcattcagttcctacaaatttcttcgcagagctattcggtggaggtgaattggatcgtagagttatggagaaagctggttgtcttaactattctcacaccccatgggaatcagagaagggcgatgtctatgtgaggcaaatatattacagatatgacaaacgtgtttcccaatatagaggagaggtgactagtactcagcaaaaatcccgcctttctgatagaaatggttggctttttcaagaagtcatgaccctccatgcaattccactcggtgactatttcaatgttcacatccgataccaaattgaggactcatcctcaacaccaccagggtgtctggtaaaagtatactttggcattgagtggctcaaaagcactaagcatcagaaaagaattacaaaaaatgttctcaagaatctgcaagatcgcctaaaggtctcctttgctgtagttgagaaggagttcacatcaagatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 DUF4782 GRAM

Protein Analysis

1026

Amino Acids

115.48

Weight (kDa)

6.07

Isoelectric Point (pI)

47.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 2 - 96 2.4e-29 C2 domain
VASt PF16016 266 - 412 4.5e-32 VAD1 Analog of StAR-related lipid transfer domain
C2 PF00168 547 - 648 4e-16 C2 domain
GRAM PF02893 700 - 807 8.4e-17 GRAM domain
VASt PF16016 872 - 1006 7.6e-23 VAD1 Analog of StAR-related lipid transfer domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1613
Acc36I ACCTGC 1 cut(s) 894
AccB1I GGYRCC 1 cut(s) 303
AccB7I CCANNNNNTGG 1 cut(s) 1330
AccI GTMKAC 2 cut(s) 168, 2952
AccII CGCG 1 cut(s) 753
AciI CCGC 5 cut(s) 348, 402, 2188, 2341, 2812
AclI AACGTT 1 cut(s) 1281
AclWI GGATC 8 cut(s) 57, 62, 708, 1305, 1318, 1697, 2524, 2664
AcoI YGGCCR 1 cut(s) 571
AcuI CTGAAG 2 cut(s) 2423, 2562
AdeI CACNNNGTG 1 cut(s) 2876
AfaI GTAC 9 cut(s) 74, 218, 379, 1377, 1437, 1539, 1557, 2076, 2797
AfiI CCNNNNNNNGG 9 cut(s) 142, 143, 494, 1330, 1946, 2183, 2266, 2467, 2911
AflIII ACRYGT 3 cut(s) 1552, 2153, 2764
AhlI ACTAGT 1 cut(s) 2792
AjnI CCWGG 7 cut(s) 917, 1120, 1169, 1323, 1446, 1533, 2932
AleI CACNNNNGTG 1 cut(s) 2935
Alw21I GWGCWC 3 cut(s) 189, 1196, 1228
Alw26I GTCTC 2 cut(s) 1408, 3046
AlwI GGATC 8 cut(s) 57, 62, 708, 1305, 1318, 1697, 2524, 2664
Ama87I CYCGRG 3 cut(s) 30, 773, 1623
AoxI GGCC 9 cut(s) 571, 669, 684, 1124, 1584, 1879, 2185, 2457, 2563
ApeKI GCWGC 4 cut(s) 240, 968, 1132, 1676
AseI ATTAAT 1 cut(s) 980
Asp700I GAANNNNTTC 2 cut(s) 2280, 3065
AspLEI GCGC 1 cut(s) 1176
AsuC2I CCSGG 1 cut(s) 786
AsuNHI GCTAGC 1 cut(s) 971
AvaI CYCGRG 3 cut(s) 30, 773, 1623
AvaII GGWCC 5 cut(s) 914, 1006, 1095, 1321, 1853
BamHI GGATCC 1 cut(s) 1310
BanI GGYRCC 1 cut(s) 303
BanII GRGCYC 3 cut(s) 31, 189, 1228
BbsI GAAGAC 2 cut(s) 131, 259
Bbv12I GWGCWC 3 cut(s) 189, 1196, 1228
BbvI GCAGC 4 cut(s) 227, 980, 1119, 1688
BccI CCATC 8 cut(s) 16, 82, 404, 1022, 1144, 1625, 1844, 2038
BceAI ACGGC 1 cut(s) 489
BciT130I CCWGG 7 cut(s) 919, 1122, 1171, 1325, 1448, 1535, 2934
BciVI GTATCC 1 cut(s) 2437
BclI TGATCA 1 cut(s) 415
BcnI CCSGG 1 cut(s) 786
BcoDI GTCTC 2 cut(s) 1408, 3046
BcuI ACTAGT 1 cut(s) 2792
BfaI CTAG 7 cut(s) 633, 800, 972, 1737, 1743, 2472, 2793
BfmI CTRYAG 6 cut(s) 897, 1187, 1680, 2180, 2325, 3051
BfuAI ACCTGC 1 cut(s) 894
BfuI GTATCC 1 cut(s) 2437
BglII AGATCT 2 cut(s) 34, 2474
BisI GCNGC 7 cut(s) 241, 349, 969, 1133, 1677, 2188, 2342
BlsI GCNGC 7 cut(s) 242, 350, 970, 1134, 1678, 2189, 2343
BmcAI AGTACT 2 cut(s) 218, 2797
Bme1390I CCNGG 8 cut(s) 786, 919, 1122, 1171, 1325, 1448, 1535, 2934
Bme18I GGWCC 5 cut(s) 914, 1006, 1095, 1321, 1853
BmeT110I CYCGRG 3 cut(s) 30, 773, 1623
BmiI GGNNCC 4 cut(s) 305, 916, 1312, 2219
BmrFI CCNGG 8 cut(s) 786, 919, 1122, 1171, 1325, 1448, 1535, 2934
BmrI ACTGGG 2 cut(s) 1348, 1568
BmsI GCATC 5 cut(s) 262, 1343, 1789, 2350, 2995
BmtI GCTAGC 1 cut(s) 975
BmuI ACTGGG 2 cut(s) 1348, 1568
BpiI GAAGAC 2 cut(s) 131, 259
BplI GAGNNNNNCTC 2 cut(s) 689, 721
BpmI CTGGAG 1 cut(s) 296
Bpu10I CCTNAGC 3 cut(s) 1128, 1959, 2487
BpuEI CTTGAG 3 cut(s) 442, 2005, 2999
BpuMI CCSGG 1 cut(s) 786
BsaAI YACGTR 2 cut(s) 76, 1711
BsaBI GATNNNNATC 1 cut(s) 414
BsaI GGTCTC 2 cut(s) 1408, 3046
BsaJI CCNNGG 9 cut(s) 522, 666, 917, 1121, 1306, 1609, 1624, 2705, 2933
BsaWI WCCGGW 1 cut(s) 853
BsaXI ACNNNNNCTCC 2 cut(s) 1539, 1569
Bsc4I CCNNNNNNNGG 9 cut(s) 142, 143, 494, 1330, 1946, 2183, 2266, 2467, 2911
Bse118I RCCGGY 1 cut(s) 40
Bse1I ACTGG 3 cut(s) 1354, 1563, 2416
Bse3DI GCAATG 5 cut(s) 51, 1337, 1732, 1809, 2023
Bse8I GATNNNNATC 1 cut(s) 414
BseBI CCWGG 7 cut(s) 919, 1122, 1171, 1325, 1448, 1535, 2934
BseDI CCNNGG 9 cut(s) 522, 666, 917, 1121, 1306, 1609, 1624, 2705, 2933
BseGI GGATG 8 cut(s) 211, 1813, 1834, 2365, 2392, 2597, 2895, 2918
BseJI GATNNNNATC 1 cut(s) 414
BseLI CCNNNNNNNGG 9 cut(s) 142, 143, 494, 1330, 1946, 2183, 2266, 2467, 2911
BseMI GCAATG 5 cut(s) 51, 1337, 1732, 1809, 2023
BseNI ACTGG 3 cut(s) 1354, 1563, 2416
BseRI GAGGAG 5 cut(s) 179, 697, 1641, 2073, 2796
BseXI GCAGC 4 cut(s) 227, 980, 1119, 1688
BseYI CCCAGC 1 cut(s) 1457
BsgI GTGCAG 1 cut(s) 1613
Bsh1236I CGCG 1 cut(s) 753
BshFI GGCC 9 cut(s) 573, 671, 686, 1126, 1586, 1881, 2187, 2459, 2565
BshNI GGYRCC 1 cut(s) 303
BsiHKAI GWGCWC 3 cut(s) 189, 1196, 1228
BsiHKCI CYCGRG 3 cut(s) 30, 773, 1623
BsiSI CCGG 3 cut(s) 41, 785, 854
BsiWI CGTACG 1 cut(s) 72
BslI CCNNNNNNNGG 9 cut(s) 142, 143, 494, 1330, 1946, 2183, 2266, 2467, 2911
BsmAI GTCTC 2 cut(s) 1408, 3046
BsmI GAATGC 3 cut(s) 389, 2099, 2605
BsnI GGCC 9 cut(s) 573, 671, 686, 1126, 1586, 1881, 2187, 2459, 2565
Bso31I GGTCTC 2 cut(s) 1408, 3046
BsoBI CYCGRG 3 cut(s) 30, 773, 1623
Bsp1286I GDGCHC 4 cut(s) 31, 189, 1196, 1228
Bsp19I CCATGG 2 cut(s) 1609, 2705
BspACI CCGC 5 cut(s) 348, 402, 2188, 2341, 2812
BspANI GGCC 9 cut(s) 573, 671, 686, 1126, 1586, 1881, 2187, 2459, 2565
BspFNI CGCG 1 cut(s) 753
BspHI TCATGA 1 cut(s) 2850
BspLI GGNNCC 4 cut(s) 305, 916, 1312, 2219
BspMAI CTGCAG 2 cut(s) 1191, 2184
BspMI ACCTGC 1 cut(s) 894
BspOI GCTAGC 1 cut(s) 975
BspPI GGATC 8 cut(s) 57, 62, 708, 1305, 1318, 1697, 2524, 2664
BspQI GCTCTTC 1 cut(s) 720
BspT107I GGYRCC 1 cut(s) 303
BspTNI GGTCTC 2 cut(s) 1408, 3046
BsrDI GCAATG 5 cut(s) 51, 1337, 1732, 1809, 2023
BsrFI RCCGGY 1 cut(s) 40
BsrI ACTGG 3 cut(s) 1354, 1563, 2416
BssAI RCCGGY 1 cut(s) 40
BssECI CCNNGG 9 cut(s) 522, 666, 917, 1121, 1306, 1609, 1624, 2705, 2933
BssNAI GTATAC 1 cut(s) 2953
BssT1I CCWWGG 5 cut(s) 522, 666, 1306, 1609, 2705
Bst1107I GTATAC 1 cut(s) 2953
Bst2UI CCWGG 7 cut(s) 919, 1122, 1171, 1325, 1448, 1535, 2934
Bst4CI ACNGT 4 cut(s) 1247, 1399, 1648, 2074
Bst6I CTCTTC 4 cut(s) 144, 720, 1947, 2538
BstAPI GCANNNNNTGC 1 cut(s) 2497
BstBAI YACGTR 2 cut(s) 76, 1711
BstDSI CCRYGG 2 cut(s) 1609, 2705
BstENI CCTNNNNNAGG 4 cut(s) 492, 1944, 2264, 2465
BstF5I GGATG 8 cut(s) 211, 1813, 1834, 2365, 2392, 2597, 2895, 2918
BstFNI CGCG 1 cut(s) 753
BstHHI GCGC 1 cut(s) 1176
BstMAI GTCTC 2 cut(s) 1408, 3046
BstMWI GCNNNNNNNGC 3 cut(s) 1132, 1495, 2497
BstNI CCWGG 7 cut(s) 919, 1122, 1171, 1325, 1448, 1535, 2934
BstNSI RCATGY 2 cut(s) 2157, 2584
BstSCI CCNGG 8 cut(s) 784, 917, 1120, 1169, 1323, 1446, 1533, 2932
BstSFI CTRYAG 6 cut(s) 897, 1187, 1680, 2180, 2325, 3051
BstUI CGCG 1 cut(s) 753
BstV1I GCAGC 4 cut(s) 227, 980, 1119, 1688
BstV2I GAAGAC 2 cut(s) 131, 259
BstX2I RGATCY 5 cut(s) 34, 49, 1310, 2474, 2529
BstYI RGATCY 5 cut(s) 34, 49, 1310, 2474, 2529
BstZ17I GTATAC 1 cut(s) 2953
BsuI GTATCC 1 cut(s) 2437
BsuRI GGCC 9 cut(s) 573, 671, 686, 1126, 1586, 1881, 2187, 2459, 2565
BtgI CCRYGG 2 cut(s) 1609, 2705
BtgZI GCGATG 1 cut(s) 2739
BtsCI GGATG 8 cut(s) 211, 1813, 1834, 2365, 2392, 2597, 2895, 2918
BtsI GCAGTG 1 cut(s) 1609
BtsIMutI CAGTG 2 cut(s) 1395, 1609
BveI ACCTGC 1 cut(s) 894
CciI TCATGA 1 cut(s) 2850
CfoI GCGC 1 cut(s) 1176
Cfr10I RCCGGY 1 cut(s) 40
CseI GACGC 2 cut(s) 232, 614
CsiI ACCWGGT 1 cut(s) 1323
Csp6I GTAC 9 cut(s) 73, 217, 378, 1376, 1436, 1538, 1556, 2075, 2796
CviQI GTAC 9 cut(s) 73, 217, 378, 1376, 1436, 1538, 1556, 2075, 2796
DraI TTTAAA 3 cut(s) 1239, 1998, 2037
DraIII CACNNNGTG 1 cut(s) 2876
DrdI GACNNNNNNGTC 1 cut(s) 1613
DseDI GACNNNNNNGTC 1 cut(s) 1613
EaeI YGGCCR 1 cut(s) 571
Eam1104I CTCTTC 4 cut(s) 144, 720, 1947, 2538
EarI CTCTTC 4 cut(s) 144, 720, 1947, 2538
Ecl136II GAGCTC 2 cut(s) 187, 1226
Eco130I CCWWGG 5 cut(s) 522, 666, 1306, 1609, 2705
Eco24I GRGCYC 3 cut(s) 31, 189, 1228
Eco31I GGTCTC 2 cut(s) 1408, 3046
Eco47I GGWCC 5 cut(s) 914, 1006, 1095, 1321, 1853
Eco53kI GAGCTC 2 cut(s) 187, 1226
Eco57I CTGAAG 2 cut(s) 2423, 2562
Eco88I CYCGRG 3 cut(s) 30, 773, 1623
EcoICRI GAGCTC 2 cut(s) 187, 1226
EcoNI CCTNNNNNAGG 4 cut(s) 492, 1944, 2264, 2465
EcoO109I RGGNCCY 1 cut(s) 914
EcoRI GAATTC 1 cut(s) 1514
EcoRII CCWGG 7 cut(s) 917, 1120, 1169, 1323, 1446, 1533, 2932
EcoT14I CCWWGG 5 cut(s) 522, 666, 1306, 1609, 2705
EcoT22I ATGCAT 1 cut(s) 391
EcoT38I GRGCYC 3 cut(s) 31, 189, 1228
ErhI CCWWGG 5 cut(s) 522, 666, 1306, 1609, 2705
FalI AAGNNNNNCTT 4 cut(s) 2396, 2428, 3029, 3061
FauI CCCGC 1 cut(s) 2819
FauNDI CATATG 2 cut(s) 958, 1078
FbaI TGATCA 1 cut(s) 415
FblI GTMKAC 2 cut(s) 168, 2952
Fnu4HI GCNGC 7 cut(s) 241, 349, 969, 1133, 1677, 2188, 2342
FokI GGATG 8 cut(s) 218, 1820, 1841, 2372, 2399, 2584, 2882, 2905
FriOI GRGCYC 3 cut(s) 31, 189, 1228
Fsp4HI GCNGC 7 cut(s) 241, 349, 969, 1133, 1677, 2188, 2342
FspBI CTAG 7 cut(s) 633, 800, 972, 1737, 1743, 2472, 2793
GlaI GCGC 1 cut(s) 1175
GluI GCNGC 7 cut(s) 241, 349, 969, 1133, 1677, 2188, 2342
GsaI CCCAGC 1 cut(s) 1461
GsuI CTGGAG 1 cut(s) 296
HaeIII GGCC 9 cut(s) 573, 671, 686, 1126, 1586, 1881, 2187, 2459, 2565
HapII CCGG 3 cut(s) 41, 785, 854
HgaI GACGC 2 cut(s) 232, 614
HhaI GCGC 1 cut(s) 1176
Hin6I GCGC 1 cut(s) 1174
HinP1I GCGC 1 cut(s) 1174
HincII GTYRAC 1 cut(s) 169
HindII GTYRAC 1 cut(s) 169
HindIII AAGCTT 1 cut(s) 500
HpaII CCGG 3 cut(s) 41, 785, 854
Hpy166II GTNNAC 7 cut(s) 156, 169, 1438, 1556, 2893, 2953, 3069
Hpy8I GTNNAC 7 cut(s) 156, 169, 1438, 1556, 2893, 2953, 3069
Hpy99I CGWCG 3 cut(s) 173, 185, 230
HpyCH4III ACNGT 4 cut(s) 1247, 1399, 1648, 2074
HpyCH4IV ACGT 8 cut(s) 75, 128, 158, 1281, 1552, 1710, 2077, 2764
HpyF10VI GCNNNNNNNGC 3 cut(s) 1132, 1495, 2497
HpySE526I ACGT 8 cut(s) 75, 128, 158, 1281, 1552, 1710, 2077, 2764
HspAI GCGC 1 cut(s) 1174
KroI GCCGGC 1 cut(s) 40
KroNI GCCGGC 1 cut(s) 42
Ksp22I TGATCA 1 cut(s) 415
LguI GCTCTTC 1 cut(s) 720
LmnI GCTCC 2 cut(s) 192, 1223
Lsp1109I GCAGC 4 cut(s) 227, 980, 1119, 1688
LweI GCATC 5 cut(s) 262, 1343, 1789, 2350, 2995
MabI ACCWGGT 1 cut(s) 1323
MaeI CTAG 7 cut(s) 633, 800, 972, 1737, 1743, 2472, 2793
MaeII ACGT 8 cut(s) 75, 128, 158, 1281, 1552, 1710, 2077, 2764
MaeIII GTNAC 7 cut(s) 747, 814, 952, 1604, 2156, 2788, 2876
MfeI CAATTG 1 cut(s) 1506
MflI RGATCY 5 cut(s) 34, 49, 1310, 2474, 2529
MhlI GDGCHC 4 cut(s) 31, 189, 1196, 1228
MlyI GAGTC 7 cut(s) 174, 392, 541, 1623, 2329, 2491, 2909
MmeI TCCRAC 6 cut(s) 420, 459, 1455, 2038, 2321, 2345
Mph1103I ATGCAT 1 cut(s) 391
MroNI GCCGGC 1 cut(s) 40
MroXI GAANNNNTTC 2 cut(s) 2280, 3065
MslI CAYNNNNRTG 2 cut(s) 1199, 2935
MspA1I CMGCKG 2 cut(s) 1292, 1679
MspI CCGG 3 cut(s) 41, 785, 854
MspR9I CCNGG 8 cut(s) 786, 919, 1122, 1171, 1325, 1448, 1535, 2934
MunI CAATTG 1 cut(s) 1506
Mva1269I GAATGC 3 cut(s) 389, 2099, 2605
MvaI CCWGG 7 cut(s) 919, 1122, 1171, 1325, 1448, 1535, 2934
MvnI CGCG 1 cut(s) 753
MwoI GCNNNNNNNGC 3 cut(s) 1132, 1495, 2497
NaeI GCCGGC 1 cut(s) 42
NciI CCSGG 1 cut(s) 786
NcoI CCATGG 2 cut(s) 1609, 2705
NdeI CATATG 2 cut(s) 958, 1078
NgoMIV GCCGGC 1 cut(s) 40
NheI GCTAGC 1 cut(s) 971
NlaIV GGNNCC 4 cut(s) 305, 916, 1312, 2219
NmeAIII GCCGAG 1 cut(s) 1207
NmuCI GTSAC 4 cut(s) 1604, 2156, 2788, 2876
NsiI ATGCAT 1 cut(s) 391
NspI RCATGY 2 cut(s) 2157, 2584
OliI CACNNNNGTG 1 cut(s) 2935
PaeR7I CTCGAG 1 cut(s) 30
PagI TCATGA 1 cut(s) 2850
PciI ACATGT 1 cut(s) 2153
PciSI GCTCTTC 1 cut(s) 720
PcsI WCGNNNNNNNCGW 3 cut(s) 177, 231, 240
PctI GAATGC 3 cut(s) 389, 2099, 2605
PdiI GCCGGC 1 cut(s) 42
PdmI GAANNNNTTC 2 cut(s) 2280, 3065
Pfl23II CGTACG 1 cut(s) 72
PflMI CCANNNNNTGG 1 cut(s) 1330
PkrI GCNGC 7 cut(s) 242, 350, 970, 1134, 1678, 2189, 2343
PleI GAGTC 7 cut(s) 173, 392, 541, 1622, 2329, 2490, 2909
PpsI GAGTC 7 cut(s) 173, 392, 541, 1622, 2329, 2490, 2909
Ppu21I YACGTR 2 cut(s) 76, 1711
PpuMI RGGWCCY 1 cut(s) 914
PscI ACATGT 1 cut(s) 2153
PshBI ATTAAT 1 cut(s) 980
Psp124BI GAGCTC 2 cut(s) 189, 1228
Psp1406I AACGTT 1 cut(s) 1281
Psp5II RGGWCCY 1 cut(s) 914
Psp6I CCWGG 7 cut(s) 917, 1120, 1169, 1323, 1446, 1533, 2932
PspFI CCCAGC 1 cut(s) 1457
PspGI CCWGG 7 cut(s) 917, 1120, 1169, 1323, 1446, 1533, 2932
PspLI CGTACG 1 cut(s) 72
PspN4I GGNNCC 4 cut(s) 305, 916, 1312, 2219
PspPPI RGGWCCY 1 cut(s) 914
PstI CTGCAG 2 cut(s) 1191, 2184
PsuI RGATCY 5 cut(s) 34, 49, 1310, 2474, 2529
PvuII CAGCTG 2 cut(s) 1292, 1679
RsaI GTAC 9 cut(s) 74, 218, 379, 1377, 1437, 1539, 1557, 2076, 2797
RsaNI GTAC 9 cut(s) 73, 217, 378, 1376, 1436, 1538, 1556, 2075, 2796
RseI CAYNNNNRTG 2 cut(s) 1199, 2935
SacI GAGCTC 2 cut(s) 189, 1228
SalI GTCGAC 1 cut(s) 167
SapI GCTCTTC 1 cut(s) 720
SatI GCNGC 7 cut(s) 241, 349, 969, 1133, 1677, 2188, 2342
SbfI CCTGCAGG 1 cut(s) 2184
ScaI AGTACT 2 cut(s) 218, 2797
SchI GAGTC 7 cut(s) 174, 392, 541, 1623, 2329, 2491, 2909
ScrFI CCNGG 8 cut(s) 786, 919, 1122, 1171, 1325, 1448, 1535, 2934
SdaI CCTGCAGG 1 cut(s) 2184
SduI GDGCHC 4 cut(s) 31, 189, 1196, 1228
SexAI ACCWGGT 1 cut(s) 1323
SfaNI GCATC 5 cut(s) 262, 1343, 1789, 2350, 2995
SfcI CTRYAG 6 cut(s) 897, 1187, 1680, 2180, 2325, 3051
Sfr274I CTCGAG 1 cut(s) 30
SinI GGWCC 5 cut(s) 914, 1006, 1095, 1321, 1853
SlaI CTCGAG 1 cut(s) 30
SmiMI CAYNNNNRTG 2 cut(s) 1199, 2935
SmlI CTYRAG 4 cut(s) 30, 457, 1984, 3014
SmoI CTYRAG 4 cut(s) 30, 457, 1984, 3014
SpeI ACTAGT 1 cut(s) 2792
Sse8387I CCTGCAGG 1 cut(s) 2184
SsiI CCGC 5 cut(s) 348, 402, 2188, 2341, 2812
SspI AATATT 2 cut(s) 1382, 1788
SspMI CTAG 7 cut(s) 633, 800, 972, 1737, 1743, 2472, 2793
SstI GAGCTC 2 cut(s) 189, 1228
StyD4I CCNGG 8 cut(s) 784, 917, 1120, 1169, 1323, 1446, 1533, 2932
StyI CCWWGG 5 cut(s) 522, 666, 1306, 1609, 2705
TaaI ACNGT 4 cut(s) 1247, 1399, 1648, 2074
TaiI ACGT 8 cut(s) 78, 131, 161, 1284, 1555, 1713, 2080, 2767
TaqI TCGA 5 cut(s) 31, 163, 168, 270, 291
TaqII GACCGA 1 cut(s) 1112
TatI WGTACW 5 cut(s) 216, 377, 1375, 1555, 2795
TauI GCSGC 3 cut(s) 351, 2190, 2344
TscAI CASTG 2 cut(s) 1402, 1609
TseFI GTSAC 4 cut(s) 1604, 2156, 2788, 2876
TseI GCWGC 4 cut(s) 240, 968, 1132, 1676
Tsp45I GTSAC 4 cut(s) 1604, 2156, 2788, 2876
TspGWI ACGGA 1 cut(s) 60
TspRI CASTG 2 cut(s) 1402, 1609
Van91I CCANNNNNTGG 1 cut(s) 1330
VpaK11BI GGWCC 5 cut(s) 914, 1006, 1095, 1321, 1853
VspI ATTAAT 1 cut(s) 980
XagI CCTNNNNNAGG 4 cut(s) 492, 1944, 2264, 2465
XbaI TCTAGA 1 cut(s) 799
XceI RCATGY 2 cut(s) 2157, 2584
XcmI CCANNNNNNNNNTGG 1 cut(s) 2111
XhoI CTCGAG 1 cut(s) 30
XmiI GTMKAC 2 cut(s) 168, 2952
XmnI GAANNNNTTC 2 cut(s) 2280, 3065
XspI CTAG 7 cut(s) 633, 800, 972, 1737, 1743, 2472, 2793
ZrmI AGTACT 2 cut(s) 218, 2797
Zsp2I ATGCAT 1 cut(s) 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.