Rmu_ssc0000395.1_g000019

Protein of unknown function, DUF547

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000395.1
OG0004953
Physical Location & Seq
Forward (+)
79893 .. 81076
1184 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 894 bp
atgcaacgaagcgaggacaagcagaagagacttgttcttgaggaagaggttctgaaattgcaagaagagttggatggggagcaaacacttaatagggttcttcactgcgcacttcatggacctgctctatcacatccatgtcttctctcattgctaccacctcaggttcaggaactattttcagagctggaaatggtggaagacgagatcagtgggcttgaaagaaaagtcgaagagctgaaactgatgttgtacaaagagagagctcaaacaagagagtgggaaatgcataggaggcagtgggaacagaatcaatcattcttcagagctggaaatcagtcactgattgagggatcgagatcgcagaactacgaagcattgagaaaagaaaaaaggagagtaagggatagaagagcctctgttggttctgcttctgatatgcaaaggtggaatttcacaaaaactgatggagagattgctgaaatgtcaaggagactaagcagaagaagcagaattcaaagcattatgaacaatgaaagtggcattcagaaaccaaatgaactctcggaacagttgctcaagtgccttataggcatatttcttgaactaaaacagacctcattggaaaaagaggggtcatcatctgttgtgccaaagcttactctctcttgtatgaactcaaaagggttcatgccgaaaacctcattcaactgcaaatcatcagcgctcttcaactacaatacatcacatgttgacccttattgcattttgccagatatagatggtgctgtccgggacgttggcccatataagaacttcatccaaatcacaagaagctcgttagacatcagacgcttatcagattgttcaaatggaattgaaaaattgaggtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF547 Lzipper-MIP1

Protein Analysis

297

Amino Acids

34.37

Weight (kDa)

8.41

Isoelectric Point (pI)

64.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lzipper-MIP1 PF14389 7 - 87 6.2e-18 Leucine-zipper of ternary complex factor MIP1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 109
Acc36I ACCTGC 1 cut(s) 130
AclWI GGATC 1 cut(s) 361
AcsI RAATTY 2 cut(s) 451, 513
AcuI CTGAAG 1 cut(s) 307
AfaI GTAC 1 cut(s) 254
AfeI AGCGCT 1 cut(s) 726
AflIII ACRYGT 1 cut(s) 748
AgsI TTSAA 7 cut(s) 221, 518, 605, 709, 733, 870, 881
AluBI AGCT 6 cut(s) 187, 238, 266, 329, 658, 837
AluI AGCT 6 cut(s) 187, 238, 266, 329, 658, 837
Alw21I GWGCWC 1 cut(s) 268
Alw26I GTCTC 2 cut(s) 22, 487
AlwI GGATC 1 cut(s) 361
AlwNI CAGNNNCTG 1 cut(s) 343
Aor51HI AGCGCT 1 cut(s) 726
AoxI GGCC 1 cut(s) 802
ApoI RAATTY 2 cut(s) 451, 513
Asp700I GAANNNNTTC 1 cut(s) 48
AspLEI GCGC 2 cut(s) 110, 727
AspS9I GGNCC 2 cut(s) 119, 803
AsuC2I CCSGG 1 cut(s) 794
AvaII GGWCC 1 cut(s) 119
AxyI CCTNAGG 1 cut(s) 162
BanII GRGCYC 1 cut(s) 268
BbsI GAAGAC 2 cut(s) 134, 207
Bbv12I GWGCWC 1 cut(s) 268
BccI CCATC 3 cut(s) 68, 461, 776
BcnI CCSGG 1 cut(s) 794
BcoDI GTCTC 2 cut(s) 22, 487
BfoI RGCGCY 1 cut(s) 728
BfuAI ACCTGC 1 cut(s) 130
BglI GCCNNNNNGGC 1 cut(s) 591
Bme1390I CCNGG 1 cut(s) 794
Bme18I GGWCC 1 cut(s) 119
BmgT120I GGNCC 2 cut(s) 119, 803
BmrFI CCNGG 1 cut(s) 794
BpiI GAAGAC 2 cut(s) 134, 207
BpuEI CTTGAG 2 cut(s) 59, 563
BpuMI CCSGG 1 cut(s) 794
BsaBI GATNNNNATC 1 cut(s) 358
Bse21I CCTNAGG 1 cut(s) 162
Bse3DI GCAATG 1 cut(s) 149
Bse8I GATNNNNATC 1 cut(s) 358
BseGI GGATG 3 cut(s) 79, 133, 819
BseJI GATNNNNATC 1 cut(s) 358
BseMI GCAATG 1 cut(s) 149
BseMII CTCAG 1 cut(s) 176
BshFI GGCC 1 cut(s) 804
BsiHKAI GWGCWC 1 cut(s) 268
BsiSI CCGG 1 cut(s) 793
BslFI GGGAC 1 cut(s) 809
BsmAI GTCTC 2 cut(s) 22, 487
BsmFI GGGAC 1 cut(s) 809
BsmI GAATGC 1 cut(s) 543
BsnI GGCC 1 cut(s) 804
Bsp1286I GDGCHC 1 cut(s) 268
Bsp1407I TGTACA 1 cut(s) 252
Bsp143I GATC 3 cut(s) 207, 353, 359
BspANI GGCC 1 cut(s) 804
BspCNI CTCAG 1 cut(s) 175
BspMI ACCTGC 1 cut(s) 130
BspPI GGATC 1 cut(s) 361
BspQI GCTCTTC 3 cut(s) 228, 406, 734
BsrDI GCAATG 1 cut(s) 149
BsrGI TGTACA 1 cut(s) 252
BssMI GATC 3 cut(s) 207, 353, 359
Bst4CI ACNGT 1 cut(s) 573
Bst6I CTCTTC 6 cut(s) 20, 39, 60, 228, 406, 734
BstAUI TGTACA 1 cut(s) 252
BstDEI CTNAG 2 cut(s) 162, 497
BstF5I GGATG 3 cut(s) 79, 133, 819
BstH2I RGCGCY 1 cut(s) 728
BstHHI GCGC 2 cut(s) 110, 727
BstKTI GATC 3 cut(s) 210, 356, 362
BstMAI GTCTC 2 cut(s) 22, 487
BstMBI GATC 3 cut(s) 207, 353, 359
BstMWI GCNNNNNNNGC 3 cut(s) 295, 507, 591
BstNSI RCATGY 1 cut(s) 752
BstSCI CCNGG 1 cut(s) 792
BstV2I GAAGAC 2 cut(s) 134, 207
Bsu36I CCTNAGG 1 cut(s) 162
BsuRI GGCC 1 cut(s) 804
BtsCI GGATG 3 cut(s) 79, 133, 819
BtsI GCAGTG 2 cut(s) 103, 305
BtsIMutI CAGTG 4 cut(s) 103, 217, 305, 341
BveI ACCTGC 1 cut(s) 130
CaiI CAGNNNCTG 1 cut(s) 343
CfoI GCGC 2 cut(s) 110, 727
Cfr13I GGNCC 2 cut(s) 119, 803
CseI GACGC 1 cut(s) 861
Csp6I GTAC 1 cut(s) 253
CspCI CAANNNNNGTGG 2 cut(s) 520, 555
CviAII CATG 4 cut(s) 116, 138, 691, 749
CviJI RGCY 9 cut(s) 187, 217, 238, 266, 329, 416, 658, 804, 837
CviKI_1 RGCY 9 cut(s) 187, 217, 238, 266, 329, 416, 658, 804, 837
CviQI GTAC 1 cut(s) 253
DdeI CTNAG 2 cut(s) 162, 497
DpnI GATC 3 cut(s) 209, 355, 361
DpnII GATC 3 cut(s) 207, 353, 359
Eam1104I CTCTTC 6 cut(s) 20, 39, 60, 228, 406, 734
EarI CTCTTC 6 cut(s) 20, 39, 60, 228, 406, 734
Ecl136II GAGCTC 1 cut(s) 266
Eco24I GRGCYC 1 cut(s) 268
Eco47I GGWCC 1 cut(s) 119
Eco47III AGCGCT 1 cut(s) 726
Eco53kI GAGCTC 1 cut(s) 266
Eco57I CTGAAG 1 cut(s) 307
Eco81I CCTNAGG 1 cut(s) 162
EcoICRI GAGCTC 1 cut(s) 266
EcoRI GAATTC 1 cut(s) 513
EcoT22I ATGCAT 1 cut(s) 291
EcoT38I GRGCYC 1 cut(s) 268
FaeI CATG 4 cut(s) 119, 141, 694, 752
FaqI GGGAC 1 cut(s) 809
FatI CATG 4 cut(s) 115, 137, 690, 748
FokI GGATG 3 cut(s) 86, 120, 806
FriOI GRGCYC 1 cut(s) 268
FspI TGCGCA 1 cut(s) 109
GlaI GCGC 2 cut(s) 109, 726
HaeII RGCGCY 1 cut(s) 728
HaeIII GGCC 1 cut(s) 804
HapII CCGG 1 cut(s) 793
HgaI GACGC 1 cut(s) 861
HhaI GCGC 2 cut(s) 110, 727
Hin1II CATG 4 cut(s) 119, 141, 694, 752
Hin6I GCGC 2 cut(s) 108, 725
HinP1I GCGC 2 cut(s) 108, 725
HincII GTYRAC 1 cut(s) 754
HindII GTYRAC 1 cut(s) 754
HindIII AAGCTT 1 cut(s) 656
HinfI GANTC 1 cut(s) 310
HpaII CCGG 1 cut(s) 793
Hpy166II GTNNAC 1 cut(s) 754
Hpy188I TCNGA 8 cut(s) 54, 184, 326, 436, 549, 568, 851, 862
Hpy188III TCNNGA 4 cut(s) 38, 170, 357, 602
Hpy8I GTNNAC 1 cut(s) 754
HpyCH4III ACNGT 1 cut(s) 573
HpyCH4IV ACGT 1 cut(s) 798
HpyCH4V TGCA 6 cut(s) 4, 61, 289, 442, 714, 765
HpyF10VI GCNNNNNNNGC 3 cut(s) 295, 507, 591
HpyF3I CTNAG 2 cut(s) 162, 497
HpySE526I ACGT 1 cut(s) 798
Hsp92II CATG 4 cut(s) 119, 141, 694, 752
HspAI GCGC 2 cut(s) 108, 725
Kzo9I GATC 3 cut(s) 207, 353, 359
LguI GCTCTTC 3 cut(s) 228, 406, 734
LmnI GCTCC 1 cut(s) 79
LpnPI CCDG 7 cut(s) 135, 149, 155, 173, 315, 786, 806
MaeII ACGT 1 cut(s) 798
MaeIII GTNAC 1 cut(s) 339
MalI GATC 3 cut(s) 209, 355, 361
MboI GATC 3 cut(s) 207, 353, 359
MhlI GDGCHC 1 cut(s) 268
MluCI AATT 5 cut(s) 56, 451, 513, 876, 884
MmeI TCCRAC 1 cut(s) 51
Mph1103I ATGCAT 1 cut(s) 291
MroXI GAANNNNTTC 1 cut(s) 48
MseI TTAA 1 cut(s) 90
MslI CAYNNNNRTG 1 cut(s) 136
MspI CCGG 1 cut(s) 793
MspR9I CCNGG 1 cut(s) 794
Mva1269I GAATGC 1 cut(s) 543
MwoI GCNNNNNNNGC 3 cut(s) 295, 507, 591
NciI CCSGG 1 cut(s) 794
NdeII GATC 3 cut(s) 207, 353, 359
NlaIII CATG 4 cut(s) 119, 141, 694, 752
NmuCI GTSAC 1 cut(s) 339
NsbI TGCGCA 1 cut(s) 109
NsiI ATGCAT 1 cut(s) 291
NspI RCATGY 1 cut(s) 752
PciI ACATGT 1 cut(s) 748
PciSI GCTCTTC 3 cut(s) 228, 406, 734
PctI GAATGC 1 cut(s) 543
PdmI GAANNNNTTC 1 cut(s) 48
PfeI GAWTC 1 cut(s) 310
PfoI TCCNGGA 1 cut(s) 792
PscI ACATGT 1 cut(s) 748
Psp124BI GAGCTC 1 cut(s) 268
PspPI GGNCC 2 cut(s) 119, 803
PstNI CAGNNNCTG 1 cut(s) 343
RsaI GTAC 1 cut(s) 254
RsaNI GTAC 1 cut(s) 253
RseI CAYNNNNRTG 1 cut(s) 136
SacI GAGCTC 1 cut(s) 268
SapI GCTCTTC 3 cut(s) 228, 406, 734
SaqAI TTAA 1 cut(s) 90
Sau3AI GATC 3 cut(s) 207, 353, 359
Sau96I GGNCC 2 cut(s) 119, 803
ScrFI CCNGG 1 cut(s) 794
SduI GDGCHC 1 cut(s) 268
SinI GGWCC 1 cut(s) 119
SmiMI CAYNNNNRTG 1 cut(s) 136
SmlI CTYRAG 2 cut(s) 38, 578
SmoI CTYRAG 2 cut(s) 38, 578
Sse9I AATT 5 cut(s) 56, 451, 513, 876, 884
SstI GAGCTC 1 cut(s) 268
StyD4I CCNGG 1 cut(s) 792
TaaI ACNGT 1 cut(s) 573
TaiI ACGT 1 cut(s) 801
TaqI TCGA 2 cut(s) 231, 356
TasI AATT 5 cut(s) 56, 451, 513, 876, 884
TatI WGTACW 1 cut(s) 252
TfiI GAWTC 1 cut(s) 310
Tru1I TTAA 1 cut(s) 90
Tru9I TTAA 1 cut(s) 90
TscAI CASTG 4 cut(s) 110, 217, 305, 348
TseFI GTSAC 1 cut(s) 339
Tsp45I GTSAC 1 cut(s) 339
TspDTI ATGAA 7 cut(s) 104, 542, 549, 573, 679, 689, 808
TspRI CASTG 4 cut(s) 110, 217, 305, 348
VpaK11BI GGWCC 1 cut(s) 119
XapI RAATTY 2 cut(s) 451, 513
XceI RCATGY 1 cut(s) 752
XmnI GAANNNNTTC 1 cut(s) 48
Zsp2I ATGCAT 1 cut(s) 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.