Rmu_ssc0000397.1_g000049

protein phosphatase 2C 22

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000397.1
OG0008007
Physical Location & Seq
Reverse (-)
192860 .. 194560
1701 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 984 bp
atgaggacgaaggcgtcagacgtcactgttgaactgggagtagaagaagagaagtatgaatcgaagttcattccaattgtgcgctctggagcatggactgacattggttctcgctcaaacatggaagatgtttatgtctgtgtggacaattttatgcatgactatggtctcaagaatttcactgatggccccagtggcttctacggggtctttgatggacatggaggaaagcatgccgctgactttgctagctctaatttgccaaagttcatttttgaggatgaagacttccctggtgatattcaaaaggttgtaagttcagcatttctgcaaactgacactgcctttcaagaagcctgcactttggatgctacccttgcttctggtaccactgcattgacagcgcttcttgttggaaggctgttggtggtagctaatgctggagactgtcgagcagtgctttgccgccgtggaaaagcaattgcgatgtcaagcgatcacaaacctatatgcagcaaagagatcaaacgcgtcgaggcatgtggaggatcagtgtatgatggctaccttaatgggcagcttaatgtcactcgcgctttgggagactggcatatggaaggaatgaagagcagggatggtgggccactcagtgcagagccagaggttaagactgtaaaactaacggaggaagacgagttccttatcattggctgtgatgggatctgggatgtgtttaggagtcaaaatgcagtggattttgctcgtcgcaggcttcaggagcacaatgacccagccacatgtagcaaggatttagttgatgaggctttgaagaggaagagtagcgacaatctggctgttgtggtagtttgcttccaacacgagcctccacccaatttgattgcccctcgctcaagagtgcagaggagcttctctgctgaaggtttgagggagttgcaaagcttcttggatgacttgaaaacttga

Protein Analysis

327

Amino Acids

35.93

Weight (kDa)

5.07

Isoelectric Point (pI)

48.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 36 - 283 5.2e-70 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 13
AatII GACGTC 1 cut(s) 24
Acc65I GGTACC 1 cut(s) 386
AccB1I GGYRCC 1 cut(s) 386
AccII CGCG 2 cut(s) 531, 594
AciI CCGC 2 cut(s) 237, 466
AclWI GGATC 2 cut(s) 556, 728
AcsI RAATTY 1 cut(s) 175
AcuI CTGAAG 2 cut(s) 758, 957
AcyI GRCGYC 2 cut(s) 14, 21
AdeI CACNNNGTG 1 cut(s) 650
AfaI GTAC 1 cut(s) 388
AfeI AGCGCT 1 cut(s) 405
AflIII ACRYGT 2 cut(s) 529, 797
AgsI TTSAA 5 cut(s) 32, 305, 350, 829, 976
AjnI CCWGG 1 cut(s) 292
AloI GAACNNNNNNTCC 2 cut(s) 680, 712
AluBI AGCT 5 cut(s) 252, 434, 580, 927, 960
AluI AGCT 5 cut(s) 252, 434, 580, 927, 960
Alw21I GWGCWC 1 cut(s) 783
Alw26I GTCTC 3 cut(s) 173, 438, 597
AlwI GGATC 2 cut(s) 556, 728
Aor51HI AGCGCT 1 cut(s) 405
AoxI GGCC 2 cut(s) 187, 641
ApeKI GCWGC 2 cut(s) 513, 577
ApoI RAATTY 1 cut(s) 175
Asp718I GGTACC 1 cut(s) 386
AspLEI GCGC 3 cut(s) 84, 406, 596
AspS9I GGNCC 2 cut(s) 188, 641
AsuHPI GGTGA 1 cut(s) 308
AsuNHI GCTAGC 1 cut(s) 248
BanI GGYRCC 1 cut(s) 386
BauI CACGAG 1 cut(s) 878
BbsI GAAGAC 2 cut(s) 291, 696
Bbv12I GWGCWC 1 cut(s) 783
BbvI GCAGC 2 cut(s) 525, 589
BccI CCATC 5 cut(s) 179, 209, 554, 629, 710
BceAI ACGGC 1 cut(s) 453
BciT130I CCWGG 1 cut(s) 294
BcoDI GTCTC 3 cut(s) 173, 438, 597
BfaI CTAG 1 cut(s) 249
BfoI RGCGCY 1 cut(s) 407
BglI GCCNNNNNGGC 1 cut(s) 195
BisI GCNGC 4 cut(s) 237, 466, 514, 578
BlsI GCNGC 4 cut(s) 238, 467, 515, 579
Bme1390I CCNGG 1 cut(s) 294
BmgT120I GGNCC 2 cut(s) 188, 641
BmiI GGNNCC 2 cut(s) 190, 388
BmrFI CCNGG 1 cut(s) 294
BmrI ACTGGG 2 cut(s) 44, 186
BmsI GCATC 1 cut(s) 358
BmtI GCTAGC 1 cut(s) 252
BmuI ACTGGG 2 cut(s) 44, 186
BoxI GACNNNNGTC 1 cut(s) 165
BpiI GAAGAC 2 cut(s) 291, 696
BpmI CTGGAG 2 cut(s) 108, 462
BpuEI CTTGAG 2 cut(s) 155, 895
BsaHI GRCGYC 2 cut(s) 14, 21
BsaI GGTCTC 1 cut(s) 173
BsaJI CCNNGG 2 cut(s) 292, 469
BsaXI ACNNNNNCTCC 2 cut(s) 537, 567
Bse1I ACTGG 3 cut(s) 39, 192, 611
BseBI CCWGG 1 cut(s) 294
BseDI CCNNGG 2 cut(s) 292, 469
BseGI GGATG 5 cut(s) 286, 373, 640, 733, 973
BseMII CTCAG 1 cut(s) 661
BseNI ACTGG 3 cut(s) 39, 192, 611
BseRI GAGGAG 1 cut(s) 937
BseXI GCAGC 2 cut(s) 525, 589
BseYI CCCAGC 1 cut(s) 790
BsgI GTGCAG 3 cut(s) 343, 672, 938
Bsh1236I CGCG 2 cut(s) 531, 594
BshFI GGCC 2 cut(s) 189, 643
BshNI GGYRCC 1 cut(s) 386
BsiHKAI GWGCWC 1 cut(s) 783
BsmAI GTCTC 3 cut(s) 173, 438, 597
BsnI GGCC 2 cut(s) 189, 643
Bso31I GGTCTC 1 cut(s) 173
Bsp1286I GDGCHC 1 cut(s) 783
Bsp143I GATC 4 cut(s) 496, 522, 548, 720
BspACI CCGC 2 cut(s) 237, 466
BspANI GGCC 2 cut(s) 189, 643
BspCNI CTCAG 1 cut(s) 660
BspFNI CGCG 2 cut(s) 531, 594
BspLI GGNNCC 2 cut(s) 190, 388
BspOI GCTAGC 1 cut(s) 252
BspPI GGATC 2 cut(s) 556, 728
BspQI GCTCTTC 1 cut(s) 620
BspT107I GGYRCC 1 cut(s) 386
BspTNI GGTCTC 1 cut(s) 173
BsrI ACTGG 3 cut(s) 39, 192, 611
BssECI CCNNGG 2 cut(s) 292, 469
BssMI GATC 4 cut(s) 496, 522, 548, 720
BssNI GRCGYC 2 cut(s) 14, 21
BssSI CACGAG 1 cut(s) 878
Bst2BI CACGAG 1 cut(s) 878
Bst2UI CCWGG 1 cut(s) 294
Bst4CI ACNGT 3 cut(s) 28, 449, 673
Bst6I CTCTTC 4 cut(s) 42, 620, 824, 830
BstACI GRCGYC 2 cut(s) 14, 21
BstC8I GCNNGC 4 cut(s) 234, 250, 358, 770
BstDEI CTNAG 1 cut(s) 647
BstDSI CCRYGG 1 cut(s) 469
BstF5I GGATG 5 cut(s) 286, 373, 640, 733, 973
BstFNI CGCG 2 cut(s) 531, 594
BstH2I RGCGCY 1 cut(s) 407
BstHHI GCGC 3 cut(s) 84, 406, 596
BstKTI GATC 4 cut(s) 499, 525, 551, 723
BstMAI GTCTC 3 cut(s) 173, 438, 597
BstMBI GATC 4 cut(s) 496, 522, 548, 720
BstMWI GCNNNNNNNGC 5 cut(s) 195, 245, 377, 401, 778
BstNI CCWGG 1 cut(s) 294
BstNSI RCATGY 3 cut(s) 236, 543, 801
BstPAI GACNNNNGTC 1 cut(s) 165
BstSCI CCNGG 1 cut(s) 292
BstUI CGCG 2 cut(s) 531, 594
BstV1I GCAGC 2 cut(s) 525, 589
BstV2I GAAGAC 2 cut(s) 291, 696
BstX2I RGATCY 1 cut(s) 720
BstYI RGATCY 1 cut(s) 720
BsuRI GGCC 2 cut(s) 189, 643
BtgI CCRYGG 1 cut(s) 469
BtgZI GCGATG 1 cut(s) 500
BtsCI GGATG 5 cut(s) 286, 373, 640, 733, 973
BtsI GCAGTG 4 cut(s) 339, 390, 462, 756
BtsIMutI CAGTG 9 cut(s) 24, 180, 199, 339, 390, 462, 558, 655, 756
Cac8I GCNNGC 4 cut(s) 234, 250, 358, 770
CfoI GCGC 3 cut(s) 84, 406, 596
Cfr13I GGNCC 2 cut(s) 188, 641
CseI GACGC 2 cut(s) 3, 520
Csp6I GTAC 1 cut(s) 387
CspCI CAANNNNNGTGG 2 cut(s) 876, 911
CviAII CATG 7 cut(s) 93, 121, 158, 221, 233, 540, 798
CviQI GTAC 1 cut(s) 387
DdeI CTNAG 1 cut(s) 647
DpnI GATC 4 cut(s) 498, 524, 550, 722
DpnII GATC 4 cut(s) 496, 522, 548, 720
DraIII CACNNNGTG 1 cut(s) 650
DrdI GACNNNNNNGTC 1 cut(s) 13
DseDI GACNNNNNNGTC 1 cut(s) 13
Eam1104I CTCTTC 4 cut(s) 42, 620, 824, 830
EarI CTCTTC 4 cut(s) 42, 620, 824, 830
Eco31I GGTCTC 1 cut(s) 173
Eco47III AGCGCT 1 cut(s) 405
Eco57I CTGAAG 2 cut(s) 758, 957
EcoRII CCWGG 1 cut(s) 292
EcoT22I ATGCAT 1 cut(s) 159
FaeI CATG 7 cut(s) 96, 124, 161, 224, 236, 543, 801
FatI CATG 7 cut(s) 92, 120, 157, 220, 232, 539, 797
FauNDI CATATG 1 cut(s) 612
Fnu4HI GCNGC 4 cut(s) 237, 466, 514, 578
FokI GGATG 5 cut(s) 293, 380, 647, 740, 980
Fsp4HI GCNGC 4 cut(s) 237, 466, 514, 578
FspBI CTAG 1 cut(s) 249
GlaI GCGC 3 cut(s) 83, 405, 595
GluI GCNGC 4 cut(s) 237, 466, 514, 578
GsaI CCCAGC 1 cut(s) 794
GsuI CTGGAG 2 cut(s) 108, 462
HaeII RGCGCY 1 cut(s) 407
HaeIII GGCC 2 cut(s) 189, 643
HgaI GACGC 2 cut(s) 3, 520
HhaI GCGC 3 cut(s) 84, 406, 596
Hin1I GRCGYC 2 cut(s) 14, 21
Hin1II CATG 7 cut(s) 96, 124, 161, 224, 236, 543, 801
Hin6I GCGC 3 cut(s) 82, 404, 594
HinP1I GCGC 3 cut(s) 82, 404, 594
HindIII AAGCTT 1 cut(s) 958
HinfI GANTC 2 cut(s) 59, 739
HphI GGTGA 1 cut(s) 308
Hpy166II GTNNAC 1 cut(s) 145
Hpy188I TCNGA 1 cut(s) 19
Hpy188III TCNNGA 5 cut(s) 87, 172, 350, 776, 912
Hpy8I GTNNAC 1 cut(s) 145
Hpy99I CGWCG 2 cut(s) 536, 768
HpyAV CCTTC 4 cut(s) 4, 411, 611, 932
HpyCH4III ACNGT 3 cut(s) 28, 449, 673
HpyCH4IV ACGT 1 cut(s) 21
HpyCH4V TGCA 9 cut(s) 157, 331, 360, 395, 513, 653, 749, 919, 955
HpyF10VI GCNNNNNNNGC 5 cut(s) 195, 245, 377, 401, 778
HpyF3I CTNAG 1 cut(s) 647
HpySE526I ACGT 1 cut(s) 21
Hsp92I GRCGYC 2 cut(s) 14, 21
Hsp92II CATG 7 cut(s) 96, 124, 161, 224, 236, 543, 801
HspAI GCGC 3 cut(s) 82, 404, 594
KpnI GGTACC 1 cut(s) 390
Kzo9I GATC 4 cut(s) 496, 522, 548, 720
LguI GCTCTTC 1 cut(s) 620
LmnI GCTCC 3 cut(s) 89, 778, 924
Lsp1109I GCAGC 2 cut(s) 525, 589
LweI GCATC 1 cut(s) 358
MaeI CTAG 1 cut(s) 249
MaeII ACGT 1 cut(s) 21
MaeIII GTNAC 2 cut(s) 22, 586
MalI GATC 4 cut(s) 498, 524, 550, 722
MboI GATC 4 cut(s) 496, 522, 548, 720
MboII GAAGA 8 cut(s) 56, 59, 137, 296, 637, 701, 841, 847
MfeI CAATTG 2 cut(s) 75, 480
MflI RGATCY 1 cut(s) 720
MhlI GDGCHC 1 cut(s) 783
MluCI AATT 6 cut(s) 75, 148, 175, 256, 480, 892
MluI ACGCGT 1 cut(s) 529
MlyI GAGTC 1 cut(s) 748
MmeI TCCRAC 2 cut(s) 394, 898
Mph1103I ATGCAT 1 cut(s) 159
MseI TTAA 3 cut(s) 570, 582, 666
MslI CAYNNNNRTG 1 cut(s) 162
MspA1I CMGCKG 1 cut(s) 239
MspR9I CCNGG 1 cut(s) 294
MunI CAATTG 2 cut(s) 75, 480
MvaI CCWGG 1 cut(s) 294
MvnI CGCG 2 cut(s) 531, 594
MwoI GCNNNNNNNGC 5 cut(s) 195, 245, 377, 401, 778
NdeI CATATG 1 cut(s) 612
NdeII GATC 4 cut(s) 496, 522, 548, 720
NheI GCTAGC 1 cut(s) 248
NlaIII CATG 7 cut(s) 96, 124, 161, 224, 236, 543, 801
NlaIV GGNNCC 2 cut(s) 190, 388
NmuCI GTSAC 2 cut(s) 22, 586
NsiI ATGCAT 1 cut(s) 159
NspI RCATGY 3 cut(s) 236, 543, 801
PaeI GCATGC 1 cut(s) 236
PciI ACATGT 1 cut(s) 797
PciSI GCTCTTC 1 cut(s) 620
PfeI GAWTC 1 cut(s) 59
PkrI GCNGC 4 cut(s) 238, 467, 515, 579
PleI GAGTC 1 cut(s) 747
PpsI GAGTC 1 cut(s) 747
PscI ACATGT 1 cut(s) 797
PshAI GACNNNNGTC 1 cut(s) 165
Psp6I CCWGG 1 cut(s) 292
PspFI CCCAGC 1 cut(s) 790
PspGI CCWGG 1 cut(s) 292
PspN4I GGNNCC 2 cut(s) 190, 388
PspPI GGNCC 2 cut(s) 188, 641
PsuI RGATCY 1 cut(s) 720
RsaI GTAC 1 cut(s) 388
RsaNI GTAC 1 cut(s) 387
RseI CAYNNNNRTG 1 cut(s) 162
SapI GCTCTTC 1 cut(s) 620
SaqAI TTAA 3 cut(s) 570, 582, 666
SatI GCNGC 4 cut(s) 237, 466, 514, 578
Sau3AI GATC 4 cut(s) 496, 522, 548, 720
Sau96I GGNCC 2 cut(s) 188, 641
SchI GAGTC 1 cut(s) 748
ScrFI CCNGG 1 cut(s) 294
SduI GDGCHC 1 cut(s) 783
SfaNI GCATC 1 cut(s) 358
SmiMI CAYNNNNRTG 1 cut(s) 162
SmlI CTYRAG 2 cut(s) 170, 910
SmoI CTYRAG 2 cut(s) 170, 910
SphI GCATGC 1 cut(s) 236
Sse9I AATT 6 cut(s) 75, 148, 175, 256, 480, 892
SsiI CCGC 2 cut(s) 237, 466
SspMI CTAG 1 cut(s) 249
StyD4I CCNGG 1 cut(s) 292
TaaI ACNGT 3 cut(s) 28, 449, 673
TaiI ACGT 1 cut(s) 24
TaqI TCGA 3 cut(s) 62, 451, 534
TasI AATT 6 cut(s) 75, 148, 175, 256, 480, 892
TauI GCSGC 2 cut(s) 239, 468
TfiI GAWTC 1 cut(s) 59
Tru1I TTAA 3 cut(s) 570, 582, 666
Tru9I TTAA 3 cut(s) 570, 582, 666
TscAI CASTG 9 cut(s) 31, 187, 199, 346, 397, 462, 558, 655, 756
TseFI GTSAC 2 cut(s) 22, 586
TseI GCWGC 2 cut(s) 513, 577
Tsp45I GTSAC 2 cut(s) 22, 586
TspDTI ATGAA 5 cut(s) 58, 72, 259, 297, 638
TspGWI ACGGA 1 cut(s) 698
TspRI CASTG 9 cut(s) 31, 187, 199, 346, 397, 462, 558, 655, 756
XapI RAATTY 1 cut(s) 175
XceI RCATGY 3 cut(s) 236, 543, 801
XspI CTAG 1 cut(s) 249
ZraI GACGTC 1 cut(s) 22
Zsp2I ATGCAT 1 cut(s) 159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.