Rmu_ssc0000397.1_g000050

protein phosphatase 2C 22

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000397.1
OG0008007
Physical Location & Seq
Reverse (-)
194878 .. 195441
564 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 564 bp
ctgttggtggtagctaatgctggagactgtcgagcagtgctttgccgccgtggaaaagcaattgcgatgtcaagcgatcacaaacctatatgcagcaaagagatcaaacgcgtcgaggcatgtggaggatcagtgtatgatggctaccttaatgggcagcttaatgtcactcgcgctttgggagactggcatatggaaggaatgaagagcagggatggtgggccactcagtgcagagccagaggttaagactgtaaaactaacggaggaagacgagttccttatcattggctgtgatgggatctgggatgtgtttaggagtcaaaatgcagtggattttgctcgtcgcaggcttcaggagcacaatgacccagccacatgtagcaaggatttagttgatgaggctttgaagaggaagagtagcgacaatctggctgttgtggtagtttgcttccaacacgagcctccacccaatttgattgcccctcgctcaagagtgcagaggagcttctctgctgaaggtttgagggagttgcaaagcttcttggatgacttgaaaacttga

Protein Analysis

187

Amino Acids

20.71

Weight (kDa)

6.13

Isoelectric Point (pI)

57.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 2 - 143 8.9e-52 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 111, 174
AciI CCGC 1 cut(s) 46
AclWI GGATC 2 cut(s) 136, 308
AcuI CTGAAG 2 cut(s) 338, 537
AdeI CACNNNGTG 1 cut(s) 230
AflIII ACRYGT 2 cut(s) 109, 377
AgsI TTSAA 2 cut(s) 409, 556
AloI GAACNNNNNNTCC 2 cut(s) 260, 292
AluBI AGCT 4 cut(s) 14, 160, 507, 540
AluI AGCT 4 cut(s) 14, 160, 507, 540
Alw21I GWGCWC 1 cut(s) 363
Alw26I GTCTC 2 cut(s) 18, 177
AlwI GGATC 2 cut(s) 136, 308
AoxI GGCC 1 cut(s) 221
ApeKI GCWGC 2 cut(s) 93, 157
AspLEI GCGC 1 cut(s) 176
AspS9I GGNCC 1 cut(s) 221
BauI CACGAG 1 cut(s) 458
BbsI GAAGAC 1 cut(s) 276
Bbv12I GWGCWC 1 cut(s) 363
BbvI GCAGC 2 cut(s) 105, 169
BccI CCATC 3 cut(s) 134, 209, 290
BceAI ACGGC 1 cut(s) 33
BcoDI GTCTC 2 cut(s) 18, 177
BisI GCNGC 3 cut(s) 46, 94, 158
BlsI GCNGC 3 cut(s) 47, 95, 159
BmgT120I GGNCC 1 cut(s) 221
BpiI GAAGAC 1 cut(s) 276
BpmI CTGGAG 1 cut(s) 42
BpuEI CTTGAG 1 cut(s) 475
BsaJI CCNNGG 1 cut(s) 49
BsaXI ACNNNNNCTCC 2 cut(s) 117, 147
Bse1I ACTGG 1 cut(s) 191
BseDI CCNNGG 1 cut(s) 49
BseGI GGATG 3 cut(s) 220, 313, 553
BseMII CTCAG 1 cut(s) 241
BseNI ACTGG 1 cut(s) 191
BseRI GAGGAG 1 cut(s) 517
BseXI GCAGC 2 cut(s) 105, 169
BseYI CCCAGC 1 cut(s) 370
BsgI GTGCAG 2 cut(s) 252, 518
Bsh1236I CGCG 2 cut(s) 111, 174
BshFI GGCC 1 cut(s) 223
BsiHKAI GWGCWC 1 cut(s) 363
BsmAI GTCTC 2 cut(s) 18, 177
BsnI GGCC 1 cut(s) 223
Bsp1286I GDGCHC 1 cut(s) 363
Bsp143I GATC 4 cut(s) 76, 102, 128, 300
BspACI CCGC 1 cut(s) 46
BspANI GGCC 1 cut(s) 223
BspCNI CTCAG 1 cut(s) 240
BspFNI CGCG 2 cut(s) 111, 174
BspPI GGATC 2 cut(s) 136, 308
BspQI GCTCTTC 1 cut(s) 200
BsrI ACTGG 1 cut(s) 191
BssECI CCNNGG 1 cut(s) 49
BssMI GATC 4 cut(s) 76, 102, 128, 300
BssSI CACGAG 1 cut(s) 458
Bst2BI CACGAG 1 cut(s) 458
Bst4CI ACNGT 2 cut(s) 29, 253
Bst6I CTCTTC 3 cut(s) 200, 404, 410
BstC8I GCNNGC 1 cut(s) 350
BstDEI CTNAG 1 cut(s) 227
BstDSI CCRYGG 1 cut(s) 49
BstF5I GGATG 3 cut(s) 220, 313, 553
BstFNI CGCG 2 cut(s) 111, 174
BstHHI GCGC 1 cut(s) 176
BstKTI GATC 4 cut(s) 79, 105, 131, 303
BstMAI GTCTC 2 cut(s) 18, 177
BstMBI GATC 4 cut(s) 76, 102, 128, 300
BstMWI GCNNNNNNNGC 1 cut(s) 358
BstNSI RCATGY 2 cut(s) 123, 381
BstUI CGCG 2 cut(s) 111, 174
BstV1I GCAGC 2 cut(s) 105, 169
BstV2I GAAGAC 1 cut(s) 276
BstX2I RGATCY 1 cut(s) 300
BstYI RGATCY 1 cut(s) 300
BsuRI GGCC 1 cut(s) 223
BtgI CCRYGG 1 cut(s) 49
BtgZI GCGATG 1 cut(s) 80
BtsCI GGATG 3 cut(s) 220, 313, 553
BtsI GCAGTG 2 cut(s) 42, 336
BtsIMutI CAGTG 4 cut(s) 42, 138, 235, 336
Cac8I GCNNGC 1 cut(s) 350
CfoI GCGC 1 cut(s) 176
Cfr13I GGNCC 1 cut(s) 221
CseI GACGC 1 cut(s) 100
CspCI CAANNNNNGTGG 2 cut(s) 456, 491
CviAII CATG 2 cut(s) 120, 378
DdeI CTNAG 1 cut(s) 227
DpnI GATC 4 cut(s) 78, 104, 130, 302
DpnII GATC 4 cut(s) 76, 102, 128, 300
DraIII CACNNNGTG 1 cut(s) 230
Eam1104I CTCTTC 3 cut(s) 200, 404, 410
EarI CTCTTC 3 cut(s) 200, 404, 410
Eco57I CTGAAG 2 cut(s) 338, 537
FaeI CATG 2 cut(s) 123, 381
FaiI YATR 7 cut(s) 89, 91, 121, 138, 192, 194, 379
FatI CATG 2 cut(s) 119, 377
FauNDI CATATG 1 cut(s) 192
Fnu4HI GCNGC 3 cut(s) 46, 94, 158
FokI GGATG 3 cut(s) 227, 320, 560
Fsp4HI GCNGC 3 cut(s) 46, 94, 158
GlaI GCGC 1 cut(s) 175
GluI GCNGC 3 cut(s) 46, 94, 158
GsaI CCCAGC 1 cut(s) 374
GsuI CTGGAG 1 cut(s) 42
HaeIII GGCC 1 cut(s) 223
HgaI GACGC 1 cut(s) 100
HhaI GCGC 1 cut(s) 176
Hin1II CATG 2 cut(s) 123, 381
Hin6I GCGC 1 cut(s) 174
HinP1I GCGC 1 cut(s) 174
HindIII AAGCTT 1 cut(s) 538
HinfI GANTC 1 cut(s) 319
Hpy188III TCNNGA 2 cut(s) 356, 492
Hpy99I CGWCG 2 cut(s) 116, 348
HpyAV CCTTC 2 cut(s) 191, 512
HpyCH4III ACNGT 2 cut(s) 29, 253
HpyCH4V TGCA 5 cut(s) 93, 233, 329, 499, 535
HpyF10VI GCNNNNNNNGC 1 cut(s) 358
HpyF3I CTNAG 1 cut(s) 227
Hsp92II CATG 2 cut(s) 123, 381
HspAI GCGC 1 cut(s) 174
Kzo9I GATC 4 cut(s) 76, 102, 128, 300
LguI GCTCTTC 1 cut(s) 200
LmnI GCTCC 2 cut(s) 358, 504
LpnPI CCDG 9 cut(s) 6, 172, 196, 252, 289, 334, 341, 384, 416
Lsp1109I GCAGC 2 cut(s) 105, 169
MaeIII GTNAC 1 cut(s) 166
MalI GATC 4 cut(s) 78, 104, 130, 302
MboI GATC 4 cut(s) 76, 102, 128, 300
MboII GAAGA 4 cut(s) 217, 281, 421, 427
MfeI CAATTG 1 cut(s) 60
MflI RGATCY 1 cut(s) 300
MhlI GDGCHC 1 cut(s) 363
MluCI AATT 2 cut(s) 60, 472
MluI ACGCGT 1 cut(s) 109
MlyI GAGTC 1 cut(s) 328
MmeI TCCRAC 1 cut(s) 478
MseI TTAA 3 cut(s) 150, 162, 246
MunI CAATTG 1 cut(s) 60
MvnI CGCG 2 cut(s) 111, 174
MwoI GCNNNNNNNGC 1 cut(s) 358
NdeI CATATG 1 cut(s) 192
NdeII GATC 4 cut(s) 76, 102, 128, 300
NlaIII CATG 2 cut(s) 123, 381
NmuCI GTSAC 1 cut(s) 166
NspI RCATGY 2 cut(s) 123, 381
PciI ACATGT 1 cut(s) 377
PciSI GCTCTTC 1 cut(s) 200
PkrI GCNGC 3 cut(s) 47, 95, 159
PleI GAGTC 1 cut(s) 327
PpsI GAGTC 1 cut(s) 327
PscI ACATGT 1 cut(s) 377
PspFI CCCAGC 1 cut(s) 370
PspPI GGNCC 1 cut(s) 221
PsuI RGATCY 1 cut(s) 300
SapI GCTCTTC 1 cut(s) 200
SaqAI TTAA 3 cut(s) 150, 162, 246
SatI GCNGC 3 cut(s) 46, 94, 158
Sau3AI GATC 4 cut(s) 76, 102, 128, 300
Sau96I GGNCC 1 cut(s) 221
SchI GAGTC 1 cut(s) 328
SduI GDGCHC 1 cut(s) 363
SetI ASST 8 cut(s) 16, 88, 150, 162, 246, 509, 523, 542
SmlI CTYRAG 1 cut(s) 490
SmoI CTYRAG 1 cut(s) 490
Sse9I AATT 2 cut(s) 60, 472
SsiI CCGC 1 cut(s) 46
TaaI ACNGT 2 cut(s) 29, 253
TaqI TCGA 2 cut(s) 31, 114
TasI AATT 2 cut(s) 60, 472
TauI GCSGC 1 cut(s) 48
Tru1I TTAA 3 cut(s) 150, 162, 246
Tru9I TTAA 3 cut(s) 150, 162, 246
TscAI CASTG 4 cut(s) 42, 138, 235, 336
TseFI GTSAC 1 cut(s) 166
TseI GCWGC 2 cut(s) 93, 157
Tsp45I GTSAC 1 cut(s) 166
TspDTI ATGAA 1 cut(s) 218
TspGWI ACGGA 1 cut(s) 278
TspRI CASTG 4 cut(s) 42, 138, 235, 336
XceI RCATGY 2 cut(s) 123, 381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.