Rroxscaffold_163G00450820

aldo-keto reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000163
OG0015644
Physical Location & Seq
Reverse (-)
229 .. 2582
2354 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_163G00450820.1

Sequence Viewer

Length: 504 bp
ATGATTCAAGATGGGATCAAGAAATGGGAAAAAAGCTTTTATTATTGTGTTTTCACTCCTAAGCTCACATTCGGTGCCTCTCTATATTCTCGAAAGATTTCTAATTATAAGGTTTCAAAATTGGGGTTTGGATGCATGGGTTTGACTTTTGTCAAGTATCCTGGTGTTGAAGAGGATGGAATCTCCATTATAAAGCATGCCTTCAGTAAAGGAATCACTTTCTTTGACACATCTGATATATATGGAACTGATCATGCCAATGAAATTCTTGTTGGCAAGGCCTTGAAGCAGTTGCCAAGAGAAAAGGTTCAATTAGCTTCAAAGTTTGGTGTTGTAAGCTTAGGACCTCGTTTGGTGGTAAAAGGAACTCCGGAGTATGTCCGATCATGCTGCGAGGCTAGTTTGAAGCGTCTTGGTGTCGACTACATTGATCTCTACTATTATCACCGCATTGACCAAACGGTGCCAATAGAGGAAACTGTGAGTTTGAAATCATGCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

167

Amino Acids

18.85

Weight (kDa)

9.0

Isoelectric Point (pI)

33.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 40 - 162 2.2e-27 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 108, 191
AccB1I GGYRCC 2 cut(s) 74, 463
AccI GTMKAC 1 cut(s) 420
AccIII TCCGGA 1 cut(s) 370
AciI CCGC 1 cut(s) 448
AclWI GGATC 1 cut(s) 23
AcsI RAATTY 1 cut(s) 264
AcuI CTGAAG 1 cut(s) 187
AgsI TTSAA 8 cut(s) 8, 117, 170, 286, 311, 321, 406, 490
AjnI CCWGG 1 cut(s) 160
AjuI GAANNNNNNNTTGG 2 cut(s) 255, 287
AluBI AGCT 4 cut(s) 36, 64, 317, 339
AluI AGCT 4 cut(s) 36, 64, 317, 339
AlwI GGATC 1 cut(s) 23
Aor13HI TCCGGA 1 cut(s) 370
AoxI GGCC 1 cut(s) 279
ApeKI GCWGC 1 cut(s) 390
ApoI RAATTY 1 cut(s) 264
Asp700I GAANNNNTTC 2 cut(s) 97, 306
AspS9I GGNCC 1 cut(s) 344
AsuHPI GGTGA 1 cut(s) 437
AvaII GGWCC 1 cut(s) 344
BanI GGYRCC 2 cut(s) 74, 463
BbvI GCAGC 1 cut(s) 377
BccI CCATC 2 cut(s) 5, 170
BcgI CGANNNNNNTGC 2 cut(s) 372, 406
BciT130I CCWGG 1 cut(s) 162
BciVI GTATCC 1 cut(s) 168
BclI TGATCA 1 cut(s) 250
BfaI CTAG 1 cut(s) 399
BfuI GTATCC 1 cut(s) 168
BisI GCNGC 1 cut(s) 391
BlsI GCNGC 1 cut(s) 392
Bme1390I CCNGG 1 cut(s) 162
Bme18I GGWCC 1 cut(s) 344
BmgT120I GGNCC 1 cut(s) 344
BmiI GGNNCC 2 cut(s) 76, 465
BmrFI CCNGG 1 cut(s) 162
BmsI GCATC 1 cut(s) 122
BoxI GACNNNNGTC 1 cut(s) 149
Bpu10I CCTNAGC 2 cut(s) 60, 340
BsaWI WCCGGW 1 cut(s) 370
BseAI TCCGGA 1 cut(s) 370
BseBI CCWGG 1 cut(s) 162
BseGI GGATG 2 cut(s) 137, 181
BseXI GCAGC 1 cut(s) 377
BshFI GGCC 1 cut(s) 281
BshNI GGYRCC 2 cut(s) 74, 463
BsiSI CCGG 1 cut(s) 371
BsnI GGCC 1 cut(s) 281
Bsp13I TCCGGA 1 cut(s) 370
Bsp143I GATC 4 cut(s) 15, 250, 383, 430
BspACI CCGC 1 cut(s) 448
BspANI GGCC 1 cut(s) 281
BspEI TCCGGA 1 cut(s) 370
BspLI GGNNCC 2 cut(s) 76, 465
BspPI GGATC 1 cut(s) 23
BspT107I GGYRCC 2 cut(s) 74, 463
BssMI GATC 4 cut(s) 15, 250, 383, 430
Bst2UI CCWGG 1 cut(s) 162
Bst4CI ACNGT 2 cut(s) 463, 481
Bst6I CTCTTC 1 cut(s) 165
BstC8I GCNNGC 1 cut(s) 198
BstDEI CTNAG 2 cut(s) 60, 340
BstF5I GGATG 2 cut(s) 137, 181
BstKTI GATC 4 cut(s) 18, 253, 386, 433
BstMBI GATC 4 cut(s) 15, 250, 383, 430
BstNI CCWGG 1 cut(s) 162
BstNSI RCATGY 2 cut(s) 200, 502
BstPAI GACNNNNGTC 1 cut(s) 149
BstSCI CCNGG 1 cut(s) 160
BstV1I GCAGC 1 cut(s) 377
BsuI GTATCC 1 cut(s) 168
BsuRI GGCC 1 cut(s) 281
BtsCI GGATG 2 cut(s) 137, 181
Cac8I GCNNGC 1 cut(s) 198
Cfr13I GGNCC 1 cut(s) 344
CseI GACGC 1 cut(s) 398
CviAII CATG 6 cut(s) 136, 197, 254, 387, 495, 499
CviJI RGCY 6 cut(s) 36, 64, 281, 317, 339, 398
CviKI_1 RGCY 6 cut(s) 36, 64, 281, 317, 339, 398
DdeI CTNAG 2 cut(s) 60, 340
DpnI GATC 4 cut(s) 17, 252, 385, 432
DpnII GATC 4 cut(s) 15, 250, 383, 430
Eam1104I CTCTTC 1 cut(s) 165
EarI CTCTTC 1 cut(s) 165
Eco147I AGGCCT 1 cut(s) 281
Eco47I GGWCC 1 cut(s) 344
Eco57I CTGAAG 1 cut(s) 187
EcoO109I RGGNCCY 1 cut(s) 344
EcoRII CCWGG 1 cut(s) 160
EcoT22I ATGCAT 2 cut(s) 137, 500
FaeI CATG 6 cut(s) 139, 200, 257, 390, 498, 502
FalI AAGNNNNNCTT 2 cut(s) 185, 217
FatI CATG 6 cut(s) 135, 196, 253, 386, 494, 498
FbaI TGATCA 1 cut(s) 250
FblI GTMKAC 1 cut(s) 420
Fnu4HI GCNGC 1 cut(s) 391
FokI GGATG 2 cut(s) 144, 188
Fsp4HI GCNGC 1 cut(s) 391
FspBI CTAG 1 cut(s) 399
GluI GCNGC 1 cut(s) 391
HaeIII GGCC 1 cut(s) 281
HapII CCGG 1 cut(s) 371
HgaI GACGC 1 cut(s) 398
Hin1II CATG 6 cut(s) 139, 200, 257, 390, 498, 502
HincII GTYRAC 1 cut(s) 421
HindII GTYRAC 1 cut(s) 421
HindIII AAGCTT 2 cut(s) 34, 337
HinfI GANTC 3 cut(s) 4, 180, 213
HpaII CCGG 1 cut(s) 371
HphI GGTGA 1 cut(s) 437
Hpy166II GTNNAC 1 cut(s) 421
Hpy188I TCNGA 2 cut(s) 235, 383
Hpy188III TCNNGA 4 cut(s) 8, 19, 90, 371
Hpy8I GTNNAC 1 cut(s) 421
HpyAV CCTTC 1 cut(s) 211
HpyCH4III ACNGT 2 cut(s) 463, 481
HpyCH4V TGCA 2 cut(s) 135, 498
HpyF3I CTNAG 2 cut(s) 60, 340
Hsp92II CATG 6 cut(s) 139, 200, 257, 390, 498, 502
Kpn2I TCCGGA 1 cut(s) 370
Ksp22I TGATCA 1 cut(s) 250
Kzo9I GATC 4 cut(s) 15, 250, 383, 430
LpnPI CCDG 3 cut(s) 147, 174, 384
Lsp1109I GCAGC 1 cut(s) 377
LweI GCATC 1 cut(s) 122
MaeI CTAG 1 cut(s) 399
MalI GATC 4 cut(s) 17, 252, 385, 432
MboI GATC 4 cut(s) 15, 250, 383, 430
MboII GAAGA 1 cut(s) 182
MluCI AATT 4 cut(s) 103, 119, 264, 311
MnlI CCTC 5 cut(s) 88, 166, 357, 388, 466
Mph1103I ATGCAT 2 cut(s) 137, 500
MroI TCCGGA 1 cut(s) 370
MroXI GAANNNNTTC 2 cut(s) 97, 306
MslI CAYNNNNRTG 1 cut(s) 258
MspI CCGG 1 cut(s) 371
MspR9I CCNGG 1 cut(s) 162
MvaI CCWGG 1 cut(s) 162
NdeII GATC 4 cut(s) 15, 250, 383, 430
NlaIII CATG 6 cut(s) 139, 200, 257, 390, 498, 502
NlaIV GGNNCC 2 cut(s) 76, 465
NsiI ATGCAT 2 cut(s) 137, 500
NspI RCATGY 2 cut(s) 200, 502
PaeI GCATGC 1 cut(s) 200
PceI AGGCCT 1 cut(s) 281
PdmI GAANNNNTTC 2 cut(s) 97, 306
PfeI GAWTC 3 cut(s) 4, 180, 213
PkrI GCNGC 1 cut(s) 392
PpuMI RGGWCCY 1 cut(s) 344
PshAI GACNNNNGTC 1 cut(s) 149
PsiI TTATAA 2 cut(s) 108, 191
Psp5II RGGWCCY 1 cut(s) 344
Psp6I CCWGG 1 cut(s) 160
PspGI CCWGG 1 cut(s) 160
PspN4I GGNNCC 2 cut(s) 76, 465
PspPI GGNCC 1 cut(s) 344
PspPPI RGGWCCY 1 cut(s) 344
RseI CAYNNNNRTG 1 cut(s) 258
SalI GTCGAC 1 cut(s) 419
SatI GCNGC 1 cut(s) 391
Sau3AI GATC 4 cut(s) 15, 250, 383, 430
Sau96I GGNCC 1 cut(s) 344
ScrFI CCNGG 1 cut(s) 162
SetI ASST 7 cut(s) 38, 66, 114, 309, 319, 341, 349
SfaNI GCATC 1 cut(s) 122
SinI GGWCC 1 cut(s) 344
SmiMI CAYNNNNRTG 1 cut(s) 258
SphI GCATGC 1 cut(s) 200
Sse9I AATT 4 cut(s) 103, 119, 264, 311
SseBI AGGCCT 1 cut(s) 281
SsiI CCGC 1 cut(s) 448
SspMI CTAG 1 cut(s) 399
StuI AGGCCT 1 cut(s) 281
StyD4I CCNGG 1 cut(s) 160
TaaI ACNGT 2 cut(s) 463, 481
TaqI TCGA 2 cut(s) 91, 420
TasI AATT 4 cut(s) 103, 119, 264, 311
TfiI GAWTC 3 cut(s) 4, 180, 213
TseI GCWGC 1 cut(s) 390
TspDTI ATGAA 1 cut(s) 276
VpaK11BI GGWCC 1 cut(s) 344
XapI RAATTY 1 cut(s) 264
XceI RCATGY 2 cut(s) 200, 502
XmiI GTMKAC 1 cut(s) 420
XmnI GAANNNNTTC 2 cut(s) 97, 306
XspI CTAG 1 cut(s) 399
Zsp2I ATGCAT 2 cut(s) 137, 500
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.