Rh3AG036100

aldo-keto reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0015644
Physical Location & Seq
Forward (+)
2418260 .. 2420628
2369 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG036100.1

Sequence Viewer

Length: 567 bp
ATGGCTGAACAGAAGGAAATACAGATCCCAAAGGTTAAACTTGGAACTCAAGGGTTGGAGGTTTCAAAATTGGGGTTTGGATGCGTGGGTTTGACTTTTGCCAAGTATCCTGTAAAGGAATCACGTTCTTTGACACATCTGATATATATATATGGAACTGATCATGCCAATGAAATTCTTGTTGGCAAGGCCTTGAAGCAGTTGCCAAGAGAGAAGGTTCAATTAGCCTCGAAGTTTGGTGTTGTAAGCTTAGGACCTCCTCGTGTGGTAGTAAATGGAACTCCGGAGTATGTCCGGTCATGTTGCGAGGCTAGCTTGAAGCGTCTTGGTGTCGACTACATTGATCTCTACTATTATCACCGCATCGACCAAACGGTGCCAATAGAGGAAACTATTAAGAACTTGGATACCAACATTGGCTCCTTAAGTCTAAATCTCAAGGAAGACGACCTCAAAGAAATCTCTGATGCTGTTCCACTCGACCAAGTAGCTGGTGATAAGAGTTTTGAAAGCACGAGTCATGTGCAATGGAAATTCGCCAACACTCCTGCAAAGACTCTGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

188

Amino Acids

20.88

Weight (kDa)

6.9

Isoelectric Point (pI)

32.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 50 - 136 1.8e-18 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 376
AccI GTMKAC 1 cut(s) 333
AccIII TCCGGA 1 cut(s) 283
AciI CCGC 1 cut(s) 361
AclWI GGATC 1 cut(s) 19
AcsI RAATTY 2 cut(s) 174, 533
AflII CTTAAG 1 cut(s) 424
AgsI TTSAA 5 cut(s) 66, 196, 221, 319, 509
AjuI GAANNNNNNNTTGG 2 cut(s) 165, 197
AluBI AGCT 3 cut(s) 249, 315, 491
AluI AGCT 3 cut(s) 249, 315, 491
AlwI GGATC 1 cut(s) 19
Aor13HI TCCGGA 1 cut(s) 283
AoxI GGCC 1 cut(s) 189
ApoI RAATTY 2 cut(s) 174, 533
AspS9I GGNCC 1 cut(s) 254
AsuHPI GGTGA 2 cut(s) 350, 506
AsuNHI GCTAGC 1 cut(s) 311
AvaII GGWCC 1 cut(s) 254
BanI GGYRCC 1 cut(s) 376
BauI CACGAG 2 cut(s) 261, 514
BbsI GAAGAC 1 cut(s) 450
BcgI CGANNNNNNTGC 2 cut(s) 505, 539
BciVI GTATCC 2 cut(s) 117, 400
BclI TGATCA 1 cut(s) 160
BfaI CTAG 1 cut(s) 312
BfrI CTTAAG 1 cut(s) 424
BfuI GTATCC 2 cut(s) 117, 400
Bme18I GGWCC 1 cut(s) 254
BmgT120I GGNCC 1 cut(s) 254
BmiI GGNNCC 2 cut(s) 378, 421
BmsI GCATC 3 cut(s) 71, 372, 457
BmtI GCTAGC 1 cut(s) 315
BpiI GAAGAC 1 cut(s) 450
Bpu10I CCTNAGC 1 cut(s) 250
BpuEI CTTGAG 2 cut(s) 33, 422
BsaWI WCCGGW 2 cut(s) 283, 294
BsaXI ACNNNNNCTCC 2 cut(s) 404, 434
Bse3DI GCAATG 1 cut(s) 533
BseAI TCCGGA 1 cut(s) 283
BseGI GGATG 1 cut(s) 86
BseMI GCAATG 1 cut(s) 533
BseRI GAGGAG 1 cut(s) 249
BshFI GGCC 1 cut(s) 191
BshNI GGYRCC 1 cut(s) 376
BsiSI CCGG 2 cut(s) 284, 295
BsnI GGCC 1 cut(s) 191
Bsp13I TCCGGA 1 cut(s) 283
Bsp143I GATC 3 cut(s) 24, 160, 343
BspACI CCGC 1 cut(s) 361
BspANI GGCC 1 cut(s) 191
BspEI TCCGGA 1 cut(s) 283
BspLI GGNNCC 2 cut(s) 378, 421
BspOI GCTAGC 1 cut(s) 315
BspPI GGATC 1 cut(s) 19
BspT107I GGYRCC 1 cut(s) 376
BspTI CTTAAG 1 cut(s) 424
BsrDI GCAATG 1 cut(s) 533
BssMI GATC 3 cut(s) 24, 160, 343
BssSI CACGAG 2 cut(s) 261, 514
Bst2BI CACGAG 2 cut(s) 261, 514
Bst4CI ACNGT 1 cut(s) 376
BstAFI CTTAAG 1 cut(s) 424
BstC8I GCNNGC 1 cut(s) 313
BstDEI CTNAG 1 cut(s) 250
BstF5I GGATG 1 cut(s) 86
BstKTI GATC 3 cut(s) 27, 163, 346
BstMBI GATC 3 cut(s) 24, 160, 343
BstMWI GCNNNNNNNGC 1 cut(s) 312
BstV2I GAAGAC 1 cut(s) 450
BstX2I RGATCY 1 cut(s) 24
BstXI CCANNNNNNTGG 1 cut(s) 491
BstYI RGATCY 1 cut(s) 24
BsuI GTATCC 2 cut(s) 117, 400
BsuRI GGCC 1 cut(s) 191
BtsCI GGATG 1 cut(s) 86
Cac8I GCNNGC 1 cut(s) 313
Cfr13I GGNCC 1 cut(s) 254
CseI GACGC 1 cut(s) 311
CviAII CATG 3 cut(s) 164, 300, 521
CviJI RGCY 8 cut(s) 5, 191, 227, 249, 311, 315, 420, 491
CviKI_1 RGCY 8 cut(s) 5, 191, 227, 249, 311, 315, 420, 491
DdeI CTNAG 1 cut(s) 250
DpnI GATC 3 cut(s) 26, 162, 345
DpnII GATC 3 cut(s) 24, 160, 343
Eco147I AGGCCT 1 cut(s) 191
Eco47I GGWCC 1 cut(s) 254
EcoO109I RGGNCCY 1 cut(s) 254
FaeI CATG 3 cut(s) 167, 303, 524
FaiI YATR 9 cut(s) 145, 147, 149, 151, 153, 165, 291, 301, 522
FatI CATG 3 cut(s) 163, 299, 520
FbaI TGATCA 1 cut(s) 160
FblI GTMKAC 1 cut(s) 333
FokI GGATG 1 cut(s) 93
FspBI CTAG 1 cut(s) 312
HaeIII GGCC 1 cut(s) 191
HapII CCGG 2 cut(s) 284, 295
HgaI GACGC 1 cut(s) 311
Hin1II CATG 3 cut(s) 167, 303, 524
HincII GTYRAC 1 cut(s) 334
HindII GTYRAC 1 cut(s) 334
HindIII AAGCTT 1 cut(s) 247
HinfI GANTC 3 cut(s) 119, 517, 556
HpaII CCGG 2 cut(s) 284, 295
HphI GGTGA 2 cut(s) 350, 506
Hpy166II GTNNAC 1 cut(s) 334
Hpy188I TCNGA 3 cut(s) 141, 466, 561
Hpy188III TCNNGA 1 cut(s) 284
Hpy8I GTNNAC 1 cut(s) 334
HpyAV CCTTC 2 cut(s) 7, 208
HpyCH4III ACNGT 1 cut(s) 376
HpyCH4IV ACGT 1 cut(s) 124
HpyCH4V TGCA 2 cut(s) 526, 551
HpyF10VI GCNNNNNNNGC 1 cut(s) 312
HpyF3I CTNAG 1 cut(s) 250
HpySE526I ACGT 1 cut(s) 124
Hsp92II CATG 3 cut(s) 167, 303, 524
Kpn2I TCCGGA 1 cut(s) 283
Ksp22I TGATCA 1 cut(s) 160
Kzo9I GATC 3 cut(s) 24, 160, 343
LmnI GCTCC 1 cut(s) 425
LpnPI CCDG 5 cut(s) 123, 297, 308, 477, 561
LweI GCATC 3 cut(s) 71, 372, 457
MaeI CTAG 1 cut(s) 312
MaeII ACGT 1 cut(s) 124
MalI GATC 3 cut(s) 26, 162, 345
MboI GATC 3 cut(s) 24, 160, 343
MboII GAAGA 1 cut(s) 455
MflI RGATCY 1 cut(s) 24
MluCI AATT 5 cut(s) 68, 174, 221, 533, 562
MlyI GAGTC 2 cut(s) 526, 550
MmeI TCCRAC 1 cut(s) 36
MnlI CCTC 7 cut(s) 52, 238, 267, 270, 301, 379, 461
MroI TCCGGA 1 cut(s) 283
MseI TTAA 3 cut(s) 36, 396, 425
MslI CAYNNNNRTG 1 cut(s) 168
MspCI CTTAAG 1 cut(s) 424
MspI CCGG 2 cut(s) 284, 295
MwoI GCNNNNNNNGC 1 cut(s) 312
NdeII GATC 3 cut(s) 24, 160, 343
NheI GCTAGC 1 cut(s) 311
NlaIII CATG 3 cut(s) 167, 303, 524
NlaIV GGNNCC 2 cut(s) 378, 421
PceI AGGCCT 1 cut(s) 191
PfeI GAWTC 1 cut(s) 119
PleI GAGTC 2 cut(s) 525, 550
PpsI GAGTC 2 cut(s) 525, 550
PpuMI RGGWCCY 1 cut(s) 254
Psp5II RGGWCCY 1 cut(s) 254
PspN4I GGNNCC 2 cut(s) 378, 421
PspPI GGNCC 1 cut(s) 254
PspPPI RGGWCCY 1 cut(s) 254
PsuI RGATCY 1 cut(s) 24
RseI CAYNNNNRTG 1 cut(s) 168
SalI GTCGAC 1 cut(s) 332
SaqAI TTAA 3 cut(s) 36, 396, 425
Sau3AI GATC 3 cut(s) 24, 160, 343
Sau96I GGNCC 1 cut(s) 254
SchI GAGTC 2 cut(s) 526, 550
SetI ASST 9 cut(s) 36, 63, 127, 219, 251, 259, 317, 453, 493
SfaNI GCATC 3 cut(s) 71, 372, 457
SinI GGWCC 1 cut(s) 254
SmiMI CAYNNNNRTG 1 cut(s) 168
SmlI CTYRAG 3 cut(s) 48, 424, 437
SmoI CTYRAG 3 cut(s) 48, 424, 437
Sse9I AATT 5 cut(s) 68, 174, 221, 533, 562
SseBI AGGCCT 1 cut(s) 191
SsiI CCGC 1 cut(s) 361
SspMI CTAG 1 cut(s) 312
StuI AGGCCT 1 cut(s) 191
TaaI ACNGT 1 cut(s) 376
TaiI ACGT 1 cut(s) 127
TaqI TCGA 4 cut(s) 230, 333, 366, 480
TasI AATT 5 cut(s) 68, 174, 221, 533, 562
TfiI GAWTC 1 cut(s) 119
Tru1I TTAA 3 cut(s) 36, 396, 425
Tru9I TTAA 3 cut(s) 36, 396, 425
TspDTI ATGAA 1 cut(s) 186
Vha464I CTTAAG 1 cut(s) 424
VpaK11BI GGWCC 1 cut(s) 254
XapI RAATTY 2 cut(s) 174, 533
XmiI GTMKAC 1 cut(s) 333
XspI CTAG 1 cut(s) 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.