Rroxscaffold_6G00428670

aldo-keto reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0015644
Physical Location & Seq
Forward (+)
49050341 .. 49051070
730 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00428670.1

Sequence Viewer

Length: 450 bp
ATGGCTGTCCAAATTCCTAGAGTGAAACTCGGGAGTGAGGGATTTGAGGTTTCCAAGTTGGGACTTGGTTGTTTGGGACTTAGTGGATTCTACAATGCTCCACTAGCTGAAGAGGCTGGCATATCAATAATCAAGCATGCATTCGATAATGGAATCACGTTCTTTGATACATCAGATATTTATGGACCAGGAACTAATGAAGTTTTGATCGGAAAGGCATTGAAGCAGCTGCCTCGAGATAAGGTTCAGGTTGCTACAAAGTTTGGTATTGTAAATATCAAGAAACCACAAGTTACACTTAATAGCAGTCCTGAGTATATACGCTCCTGCTGCGAGGCTAGCCTGAAGCGCCTTGATGTGGAGTACATTGATCTTTACTATCAGCACCGTGTAGACCCATTAGTACCAATAGAGGATACCGTGAGTATTACACATTTTCCCTTGGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

149

Amino Acids

16.41

Weight (kDa)

5.6

Isoelectric Point (pI)

31.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 20 - 142 4.7e-30 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 393
AcsI RAATTY 1 cut(s) 12
AcuI CTGAAG 2 cut(s) 129, 365
AfaI GTAC 2 cut(s) 365, 405
AfiI CCNNNNNNNGG 1 cut(s) 358
AgsI TTSAA 1 cut(s) 223
AjnI CCWGG 1 cut(s) 187
AluBI AGCT 2 cut(s) 107, 229
AluI AGCT 2 cut(s) 107, 229
Ama87I CYCGRG 2 cut(s) 29, 234
ApeKI GCWGC 3 cut(s) 226, 229, 330
ApoI RAATTY 1 cut(s) 12
AspLEI GCGC 1 cut(s) 351
AspS9I GGNCC 1 cut(s) 185
AsuNHI GCTAGC 1 cut(s) 338
AvaI CYCGRG 2 cut(s) 29, 234
AvaII GGWCC 1 cut(s) 185
BbvI GCAGC 3 cut(s) 216, 238, 317
BcgI CGANNNNNNTGC 2 cut(s) 215, 249
BciT130I CCWGG 1 cut(s) 189
BciVI GTATCC 1 cut(s) 409
BfaI CTAG 3 cut(s) 18, 104, 339
BfoI RGCGCY 1 cut(s) 352
BfuI GTATCC 1 cut(s) 409
BisI GCNGC 3 cut(s) 227, 230, 331
BlsI GCNGC 3 cut(s) 228, 231, 332
Bme1390I CCNGG 1 cut(s) 189
Bme18I GGWCC 1 cut(s) 185
BmeT110I CYCGRG 2 cut(s) 29, 234
BmgT120I GGNCC 1 cut(s) 185
BmrFI CCNGG 1 cut(s) 189
BmtI GCTAGC 1 cut(s) 342
BplI GAGNNNNNCTC 2 cut(s) 12, 44
BsaJI CCNNGG 1 cut(s) 441
Bsc4I CCNNNNNNNGG 1 cut(s) 358
BseBI CCWGG 1 cut(s) 189
BseDI CCNNGG 1 cut(s) 441
BseLI CCNNNNNNNGG 1 cut(s) 358
BseMII CTCAG 1 cut(s) 303
BseXI GCAGC 3 cut(s) 216, 238, 317
BsiHKCI CYCGRG 2 cut(s) 29, 234
BslFI GGGAC 2 cut(s) 75, 90
BslI CCNNNNNNNGG 1 cut(s) 358
BsmFI GGGAC 2 cut(s) 75, 90
BsmI GAATGC 1 cut(s) 140
BsoBI CYCGRG 2 cut(s) 29, 234
Bsp143I GATC 2 cut(s) 207, 370
BspCNI CTCAG 1 cut(s) 304
BspOI GCTAGC 1 cut(s) 342
BssECI CCNNGG 1 cut(s) 441
BssMI GATC 2 cut(s) 207, 370
BssT1I CCWWGG 1 cut(s) 441
Bst2UI CCWGG 1 cut(s) 189
Bst4CI ACNGT 2 cut(s) 389, 421
Bst6I CTCTTC 1 cut(s) 105
BstC8I GCNNGC 3 cut(s) 118, 138, 340
BstDEI CTNAG 2 cut(s) 80, 312
BstH2I RGCGCY 1 cut(s) 352
BstHHI GCGC 1 cut(s) 351
BstKTI GATC 2 cut(s) 210, 373
BstMBI GATC 2 cut(s) 207, 370
BstMWI GCNNNNNNNGC 5 cut(s) 104, 113, 330, 339, 348
BstNI CCWGG 1 cut(s) 189
BstNSI RCATGY 1 cut(s) 140
BstSCI CCNGG 1 cut(s) 187
BstV1I GCAGC 3 cut(s) 216, 238, 317
BsuI GTATCC 1 cut(s) 409
Cac8I GCNNGC 3 cut(s) 118, 138, 340
CfoI GCGC 1 cut(s) 351
Cfr13I GGNCC 1 cut(s) 185
Csp6I GTAC 2 cut(s) 364, 404
CviAII CATG 1 cut(s) 137
CviJI RGCY 6 cut(s) 5, 107, 116, 229, 338, 342
CviKI_1 RGCY 6 cut(s) 5, 107, 116, 229, 338, 342
CviQI GTAC 2 cut(s) 364, 404
DdeI CTNAG 2 cut(s) 80, 312
DpnI GATC 2 cut(s) 209, 372
DpnII GATC 2 cut(s) 207, 370
Eam1104I CTCTTC 1 cut(s) 105
EarI CTCTTC 1 cut(s) 105
Eco130I CCWWGG 1 cut(s) 441
Eco47I GGWCC 1 cut(s) 185
Eco57I CTGAAG 2 cut(s) 129, 365
Eco88I CYCGRG 2 cut(s) 29, 234
EcoRII CCWGG 1 cut(s) 187
EcoT14I CCWWGG 1 cut(s) 441
EcoT22I ATGCAT 1 cut(s) 142
ErhI CCWWGG 1 cut(s) 441
FaeI CATG 1 cut(s) 140
FaiI YATR 5 cut(s) 122, 138, 183, 318, 320
FalI AAGNNNNNCTT 2 cut(s) 282, 314
FaqI GGGAC 2 cut(s) 75, 90
FatI CATG 1 cut(s) 136
FblI GTMKAC 1 cut(s) 393
Fnu4HI GCNGC 3 cut(s) 227, 230, 331
Fsp4HI GCNGC 3 cut(s) 227, 230, 331
FspBI CTAG 3 cut(s) 18, 104, 339
GlaI GCGC 1 cut(s) 350
GluI GCNGC 3 cut(s) 227, 230, 331
HaeII RGCGCY 1 cut(s) 352
HhaI GCGC 1 cut(s) 351
Hin1II CATG 1 cut(s) 140
Hin6I GCGC 1 cut(s) 349
HinP1I GCGC 1 cut(s) 349
HinfI GANTC 2 cut(s) 87, 153
Hpy166II GTNNAC 1 cut(s) 394
Hpy188I TCNGA 2 cut(s) 175, 212
Hpy188III TCNNGA 4 cut(s) 31, 236, 280, 311
Hpy8I GTNNAC 1 cut(s) 394
HpyCH4III ACNGT 2 cut(s) 389, 421
HpyCH4IV ACGT 1 cut(s) 158
HpyCH4V TGCA 1 cut(s) 140
HpyF10VI GCNNNNNNNGC 5 cut(s) 104, 113, 330, 339, 348
HpyF3I CTNAG 2 cut(s) 80, 312
HpySE526I ACGT 1 cut(s) 158
Hsp92II CATG 1 cut(s) 140
HspAI GCGC 1 cut(s) 349
Kzo9I GATC 2 cut(s) 207, 370
LmnI GCTCC 2 cut(s) 103, 329
LpnPI CCDG 7 cut(s) 102, 174, 201, 233, 324, 340, 356
Lsp1109I GCAGC 3 cut(s) 216, 238, 317
MaeI CTAG 3 cut(s) 18, 104, 339
MaeII ACGT 1 cut(s) 158
MaeIII GTNAC 1 cut(s) 292
MalI GATC 2 cut(s) 209, 372
MboI GATC 2 cut(s) 207, 370
MboII GAAGA 1 cut(s) 122
MluCI AATT 1 cut(s) 12
MnlI CCTC 6 cut(s) 31, 40, 106, 243, 328, 406
Mph1103I ATGCAT 1 cut(s) 142
MseI TTAA 1 cut(s) 300
MspA1I CMGCKG 1 cut(s) 229
MspR9I CCNGG 1 cut(s) 189
Mva1269I GAATGC 1 cut(s) 140
MvaI CCWGG 1 cut(s) 189
MwoI GCNNNNNNNGC 5 cut(s) 104, 113, 330, 339, 348
NdeII GATC 2 cut(s) 207, 370
NheI GCTAGC 1 cut(s) 338
NlaIII CATG 1 cut(s) 140
NsiI ATGCAT 1 cut(s) 142
NspI RCATGY 1 cut(s) 140
PaeI GCATGC 1 cut(s) 140
PaeR7I CTCGAG 1 cut(s) 234
PctI GAATGC 1 cut(s) 140
PfeI GAWTC 2 cut(s) 87, 153
PkrI GCNGC 3 cut(s) 228, 231, 332
Psp6I CCWGG 1 cut(s) 187
PspGI CCWGG 1 cut(s) 187
PspPI GGNCC 1 cut(s) 185
PvuII CAGCTG 1 cut(s) 229
RsaI GTAC 2 cut(s) 365, 405
RsaNI GTAC 2 cut(s) 364, 404
SaqAI TTAA 1 cut(s) 300
SatI GCNGC 3 cut(s) 227, 230, 331
Sau3AI GATC 2 cut(s) 207, 370
Sau96I GGNCC 1 cut(s) 185
ScrFI CCNGG 1 cut(s) 189
SetI ASST 6 cut(s) 51, 109, 161, 231, 246, 252
Sfr274I CTCGAG 1 cut(s) 234
SinI GGWCC 1 cut(s) 185
SlaI CTCGAG 1 cut(s) 234
SmlI CTYRAG 1 cut(s) 234
SmoI CTYRAG 1 cut(s) 234
SphI GCATGC 1 cut(s) 140
Sse9I AATT 1 cut(s) 12
SspMI CTAG 3 cut(s) 18, 104, 339
StyD4I CCNGG 1 cut(s) 187
StyI CCWWGG 1 cut(s) 441
TaaI ACNGT 2 cut(s) 389, 421
TaiI ACGT 1 cut(s) 161
TaqI TCGA 2 cut(s) 144, 235
TasI AATT 1 cut(s) 12
TatI WGTACW 1 cut(s) 363
TfiI GAWTC 2 cut(s) 87, 153
Tru1I TTAA 1 cut(s) 300
Tru9I TTAA 1 cut(s) 300
TseI GCWGC 3 cut(s) 226, 229, 330
TspDTI ATGAA 1 cut(s) 213
VpaK11BI GGWCC 1 cut(s) 185
XapI RAATTY 1 cut(s) 12
XceI RCATGY 1 cut(s) 140
XhoI CTCGAG 1 cut(s) 234
XmiI GTMKAC 1 cut(s) 393
XspI CTAG 3 cut(s) 18, 104, 339
Zsp2I ATGCAT 1 cut(s) 142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.