Rh3AG036000

aldo-keto reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0015644
Physical Location & Seq
Forward (+)
2409533 .. 2413977
4445 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG036000.1

Sequence Viewer

Length: 603 bp
ATGGCTGAACAGAAGGGAGTACAGATCCCAAAGGTTAAACTCGGAACTCAAGGATTTGAGGTTTCAAAATTGGGGTTTGGATGCATGGGTTTGACTTTTGTCAAGTATCCTGGTGTTGAAGAGGATGGAATCTCCATTATAAAGCATGCCTTCAGTAAAGGAATCACTTTCTTTGACACATCTGATATATATGGAACTGATCATGCCAATGAAATTCTTGTTGGCAAGGCCTTGAAGCAGTTGCCAAGAGAAAAGGTTCAATTAGCCTCAAAGTTTGGTGTTGTAAGCTTAGGACCTCGTTTGGTGGTAAAGGGAACTCCGGAGTATGTCCGATCATGCTGCGAGGCTAGCTTGAAGCGTCTTGGTGTCGACTACATTGATCTCTACTATTATCACCGCATTGACCAAACGGTGCCAATAGAGGAAACTATTAAGAACTTGGATACCAACATTGGCTCCTTGAGTCTCAATCTCAAGGAAGACGACCTTAAAGAAATCTCTGACGCTGTTCCACTCGACCAAGTAGCTGGTGATAAGACTTTTGAATTTGCGAGTCATGTGCAATGGAAATTCGCCGACACCCCTCAAAAGACTACGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

200

Amino Acids

22.18

Weight (kDa)

5.84

Isoelectric Point (pI)

25.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 23 - 148 4e-28 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 140
AccB1I GGYRCC 1 cut(s) 412
AccI GTMKAC 1 cut(s) 369
AccIII TCCGGA 1 cut(s) 319
AciI CCGC 1 cut(s) 397
AclWI GGATC 1 cut(s) 19
AcsI RAATTY 3 cut(s) 213, 545, 569
AcuI CTGAAG 1 cut(s) 136
AfaI GTAC 1 cut(s) 21
AgsI TTSAA 6 cut(s) 66, 119, 235, 260, 355, 545
AjnI CCWGG 1 cut(s) 109
AjuI GAANNNNNNNTTGG 2 cut(s) 204, 236
AluBI AGCT 3 cut(s) 288, 351, 527
AluI AGCT 3 cut(s) 288, 351, 527
Alw26I GTCTC 1 cut(s) 470
AlwI GGATC 1 cut(s) 19
Aor13HI TCCGGA 1 cut(s) 319
AoxI GGCC 1 cut(s) 228
ApeKI GCWGC 1 cut(s) 339
ApoI RAATTY 3 cut(s) 213, 545, 569
Asp700I GAANNNNTTC 1 cut(s) 255
AspS9I GGNCC 1 cut(s) 293
AsuHPI GGTGA 2 cut(s) 386, 542
AsuNHI GCTAGC 1 cut(s) 347
AvaII GGWCC 1 cut(s) 293
BanI GGYRCC 1 cut(s) 412
BbsI GAAGAC 1 cut(s) 486
BbvI GCAGC 1 cut(s) 326
BccI CCATC 1 cut(s) 119
BcgI CGANNNNNNTGC 4 cut(s) 321, 355, 541, 575
BciT130I CCWGG 1 cut(s) 111
BciVI GTATCC 2 cut(s) 117, 436
BclI TGATCA 1 cut(s) 199
BcoDI GTCTC 1 cut(s) 470
BfaI CTAG 1 cut(s) 348
BfuI GTATCC 2 cut(s) 117, 436
BisI GCNGC 1 cut(s) 340
BlsI GCNGC 1 cut(s) 341
Bme1390I CCNGG 1 cut(s) 111
Bme18I GGWCC 1 cut(s) 293
BmgT120I GGNCC 1 cut(s) 293
BmiI GGNNCC 2 cut(s) 414, 457
BmrFI CCNGG 1 cut(s) 111
BmsI GCATC 1 cut(s) 71
BmtI GCTAGC 1 cut(s) 351
BoxI GACNNNNGTC 1 cut(s) 98
BpiI GAAGAC 1 cut(s) 486
Bpu10I CCTNAGC 1 cut(s) 289
BpuEI CTTGAG 3 cut(s) 33, 458, 481
BsaWI WCCGGW 1 cut(s) 319
BsaXI ACNNNNNCTCC 2 cut(s) 440, 470
Bse3DI GCAATG 1 cut(s) 569
BseAI TCCGGA 1 cut(s) 319
BseBI CCWGG 1 cut(s) 111
BseGI GGATG 2 cut(s) 86, 130
BseMI GCAATG 1 cut(s) 569
BseXI GCAGC 1 cut(s) 326
BshFI GGCC 1 cut(s) 230
BshNI GGYRCC 1 cut(s) 412
BsiSI CCGG 1 cut(s) 320
BsmAI GTCTC 1 cut(s) 470
BsnI GGCC 1 cut(s) 230
Bsp13I TCCGGA 1 cut(s) 319
Bsp143I GATC 4 cut(s) 24, 199, 332, 379
BspACI CCGC 1 cut(s) 397
BspANI GGCC 1 cut(s) 230
BspEI TCCGGA 1 cut(s) 319
BspLI GGNNCC 2 cut(s) 414, 457
BspOI GCTAGC 1 cut(s) 351
BspPI GGATC 1 cut(s) 19
BspT107I GGYRCC 1 cut(s) 412
BsrDI GCAATG 1 cut(s) 569
BssMI GATC 4 cut(s) 24, 199, 332, 379
Bst2UI CCWGG 1 cut(s) 111
Bst4CI ACNGT 1 cut(s) 412
Bst6I CTCTTC 1 cut(s) 114
BstC8I GCNNGC 2 cut(s) 147, 349
BstDEI CTNAG 1 cut(s) 289
BstF5I GGATG 2 cut(s) 86, 130
BstKTI GATC 4 cut(s) 27, 202, 335, 382
BstMAI GTCTC 1 cut(s) 470
BstMBI GATC 4 cut(s) 24, 199, 332, 379
BstMWI GCNNNNNNNGC 1 cut(s) 348
BstNI CCWGG 1 cut(s) 111
BstNSI RCATGY 1 cut(s) 149
BstPAI GACNNNNGTC 1 cut(s) 98
BstSCI CCNGG 1 cut(s) 109
BstV1I GCAGC 1 cut(s) 326
BstV2I GAAGAC 1 cut(s) 486
BstX2I RGATCY 1 cut(s) 24
BstXI CCANNNNNNTGG 1 cut(s) 527
BstYI RGATCY 1 cut(s) 24
BsuI GTATCC 2 cut(s) 117, 436
BsuRI GGCC 1 cut(s) 230
BtsCI GGATG 2 cut(s) 86, 130
Cac8I GCNNGC 2 cut(s) 147, 349
Cfr13I GGNCC 1 cut(s) 293
CseI GACGC 2 cut(s) 347, 512
Csp6I GTAC 1 cut(s) 20
CviAII CATG 5 cut(s) 85, 146, 203, 336, 557
CviJI RGCY 8 cut(s) 5, 230, 266, 288, 347, 351, 456, 527
CviKI_1 RGCY 8 cut(s) 5, 230, 266, 288, 347, 351, 456, 527
CviQI GTAC 1 cut(s) 20
DdeI CTNAG 1 cut(s) 289
DpnI GATC 4 cut(s) 26, 201, 334, 381
DpnII GATC 4 cut(s) 24, 199, 332, 379
Eam1104I CTCTTC 1 cut(s) 114
EarI CTCTTC 1 cut(s) 114
Eco147I AGGCCT 1 cut(s) 230
Eco47I GGWCC 1 cut(s) 293
Eco57I CTGAAG 1 cut(s) 136
EcoO109I RGGNCCY 1 cut(s) 293
EcoRII CCWGG 1 cut(s) 109
EcoT22I ATGCAT 1 cut(s) 86
FaeI CATG 5 cut(s) 88, 149, 206, 339, 560
FalI AAGNNNNNCTT 4 cut(s) 134, 166, 471, 503
FatI CATG 5 cut(s) 84, 145, 202, 335, 556
FbaI TGATCA 1 cut(s) 199
FblI GTMKAC 1 cut(s) 369
Fnu4HI GCNGC 1 cut(s) 340
FokI GGATG 2 cut(s) 93, 137
Fsp4HI GCNGC 1 cut(s) 340
FspBI CTAG 1 cut(s) 348
GluI GCNGC 1 cut(s) 340
HaeIII GGCC 1 cut(s) 230
HapII CCGG 1 cut(s) 320
HgaI GACGC 2 cut(s) 347, 512
Hin1II CATG 5 cut(s) 88, 149, 206, 339, 560
HincII GTYRAC 1 cut(s) 370
HindII GTYRAC 1 cut(s) 370
HindIII AAGCTT 1 cut(s) 286
HinfI GANTC 4 cut(s) 129, 162, 463, 553
HpaII CCGG 1 cut(s) 320
HphI GGTGA 2 cut(s) 386, 542
Hpy166II GTNNAC 1 cut(s) 370
Hpy188I TCNGA 4 cut(s) 44, 184, 332, 502
Hpy188III TCNNGA 1 cut(s) 320
Hpy8I GTNNAC 1 cut(s) 370
HpyAV CCTTC 2 cut(s) 7, 160
HpyCH4III ACNGT 1 cut(s) 412
HpyCH4V TGCA 2 cut(s) 84, 562
HpyF10VI GCNNNNNNNGC 1 cut(s) 348
HpyF3I CTNAG 1 cut(s) 289
Hsp92II CATG 5 cut(s) 88, 149, 206, 339, 560
Kpn2I TCCGGA 1 cut(s) 319
Ksp22I TGATCA 1 cut(s) 199
Kzo9I GATC 4 cut(s) 24, 199, 332, 379
LmnI GCTCC 1 cut(s) 461
LpnPI CCDG 4 cut(s) 96, 123, 333, 513
Lsp1109I GCAGC 1 cut(s) 326
LweI GCATC 1 cut(s) 71
MaeI CTAG 1 cut(s) 348
MalI GATC 4 cut(s) 26, 201, 334, 381
MboI GATC 4 cut(s) 24, 199, 332, 379
MboII GAAGA 2 cut(s) 131, 491
MflI RGATCY 1 cut(s) 24
MluCI AATT 6 cut(s) 68, 213, 260, 545, 569, 598
MlyI GAGTC 2 cut(s) 472, 562
MnlI CCTC 7 cut(s) 52, 115, 277, 306, 337, 415, 594
Mph1103I ATGCAT 1 cut(s) 86
MroI TCCGGA 1 cut(s) 319
MroXI GAANNNNTTC 1 cut(s) 255
MseI TTAA 3 cut(s) 36, 432, 489
MslI CAYNNNNRTG 1 cut(s) 207
MspI CCGG 1 cut(s) 320
MspR9I CCNGG 1 cut(s) 111
MvaI CCWGG 1 cut(s) 111
MwoI GCNNNNNNNGC 1 cut(s) 348
NdeII GATC 4 cut(s) 24, 199, 332, 379
NheI GCTAGC 1 cut(s) 347
NlaIII CATG 5 cut(s) 88, 149, 206, 339, 560
NlaIV GGNNCC 2 cut(s) 414, 457
NsiI ATGCAT 1 cut(s) 86
NspI RCATGY 1 cut(s) 149
PaeI GCATGC 1 cut(s) 149
PceI AGGCCT 1 cut(s) 230
PdmI GAANNNNTTC 1 cut(s) 255
PfeI GAWTC 2 cut(s) 129, 162
PkrI GCNGC 1 cut(s) 341
PleI GAGTC 2 cut(s) 471, 561
PpsI GAGTC 2 cut(s) 471, 561
PpuMI RGGWCCY 1 cut(s) 293
PshAI GACNNNNGTC 1 cut(s) 98
PsiI TTATAA 1 cut(s) 140
Psp5II RGGWCCY 1 cut(s) 293
Psp6I CCWGG 1 cut(s) 109
PspGI CCWGG 1 cut(s) 109
PspN4I GGNNCC 2 cut(s) 414, 457
PspPI GGNCC 1 cut(s) 293
PspPPI RGGWCCY 1 cut(s) 293
PsuI RGATCY 1 cut(s) 24
RsaI GTAC 1 cut(s) 21
RsaNI GTAC 1 cut(s) 20
RseI CAYNNNNRTG 1 cut(s) 207
SalI GTCGAC 1 cut(s) 368
SaqAI TTAA 3 cut(s) 36, 432, 489
SatI GCNGC 1 cut(s) 340
Sau3AI GATC 4 cut(s) 24, 199, 332, 379
Sau96I GGNCC 1 cut(s) 293
SchI GAGTC 2 cut(s) 472, 562
ScrFI CCNGG 1 cut(s) 111
SetI ASST 8 cut(s) 36, 63, 258, 290, 298, 353, 489, 529
SfaNI GCATC 1 cut(s) 71
SinI GGWCC 1 cut(s) 293
SmiMI CAYNNNNRTG 1 cut(s) 207
SmlI CTYRAG 3 cut(s) 48, 460, 473
SmoI CTYRAG 3 cut(s) 48, 460, 473
SphI GCATGC 1 cut(s) 149
Sse9I AATT 6 cut(s) 68, 213, 260, 545, 569, 598
SseBI AGGCCT 1 cut(s) 230
SsiI CCGC 1 cut(s) 397
SspMI CTAG 1 cut(s) 348
StuI AGGCCT 1 cut(s) 230
StyD4I CCNGG 1 cut(s) 109
TaaI ACNGT 1 cut(s) 412
TaqI TCGA 2 cut(s) 369, 516
TasI AATT 6 cut(s) 68, 213, 260, 545, 569, 598
TatI WGTACW 1 cut(s) 19
TfiI GAWTC 2 cut(s) 129, 162
Tru1I TTAA 3 cut(s) 36, 432, 489
Tru9I TTAA 3 cut(s) 36, 432, 489
TseI GCWGC 1 cut(s) 339
TspDTI ATGAA 1 cut(s) 225
VpaK11BI GGWCC 1 cut(s) 293
XapI RAATTY 3 cut(s) 213, 545, 569
XceI RCATGY 1 cut(s) 149
XmiI GTMKAC 1 cut(s) 369
XmnI GAANNNNTTC 1 cut(s) 255
XspI CTAG 1 cut(s) 348
Zsp2I ATGCAT 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.