Rroxscaffold_1G00030770

Lysine methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0004944
Physical Location & Seq
Forward (+)
41123916 .. 41135580
11665 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00030770.1

Sequence Viewer

Length: 813 bp
ATGGTGGCGGCGCTATTTTTGGTACACGGTTGGTTCTCCATTTTTCGACCACCTCTAGCCTCGCCCCGGTCGGGTTACGAAGCTTGTGAGACGGTTTTCTTTGACGTTCGAGTGGACGTATTGGTAGGTCATTGTTACCAGCTGACCACTTTCACATTTGGGTCTCAAGTTCTTCAACTCCTTTGCCTCCAATCTGCTTCCACTGATTTTGATCTTACAGGACAACTGGTGTGGCCAGGGGCAATGCTGTTGAATGATTACCTTTCTAAGAATGCAGAGATCCTGCAAGGATGCACTATTATTGAGTTAGGGTCTGGGGTTGAGAGAAGATATATAGCAATGACTATGGGAGAATGCTCTAATTTTGCAGTCAATGTTCTTTATTTGACTAGTTTAAGTTTTCTTTCTCAGATCTTGAATAAAAATATAGAGCTACACGCTTCTTCTGATAATCCTGAATACTGTGCTGGATTAGCAGCAGAGAAACTAGAATGGGGAAATTCTGAGCAGATCTCCCAGATTTTACAAAAGTATTCAAGAGGGTTCGATTTGATTCTAGGAGCTGATATATATATCCTGAATTCAACACAACAGTCAAGCATTACTTCGCTTTTTGACACAGTGGAAGAACTTCTTCGAGTTAGAAAAGAAGGAAAGTGCAAATTCATACTAGCCTATGTGTCCCGTGCTAAGACATCTGATACTAGAATTCTAATGAAGCGGGCCCGACGTAAGATGCAGATAAATGAATTAGTTGGGACTCGATCCTTCGTTGGCAATCTCGAAGGACTTATCTATGAGGTGAACCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_16

Protein Analysis

270

Amino Acids

30.26

Weight (kDa)

5.71

Isoelectric Point (pI)

49.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_16 PF10294 73 - 233 1.8e-11 Lysine methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 8, 721
AclWI GGATC 2 cut(s) 274, 759
AcoI YGGCCR 1 cut(s) 233
AcsI RAATTY 4 cut(s) 499, 580, 662, 708
AfaI GTAC 1 cut(s) 24
AfiI CCNNNNNNNGG 2 cut(s) 66, 71
AgsI TTSAA 5 cut(s) 176, 253, 418, 537, 585
AhlI ACTAGT 1 cut(s) 389
AjnI CCWGG 1 cut(s) 235
AluBI AGCT 4 cut(s) 83, 142, 433, 563
AluI AGCT 4 cut(s) 83, 142, 433, 563
Alw26I GTCTC 2 cut(s) 83, 168
AlwI GGATC 2 cut(s) 274, 759
AoxI GGCC 2 cut(s) 233, 723
ApaI GGGCCC 1 cut(s) 727
ApeKI GCWGC 1 cut(s) 476
ApoI RAATTY 4 cut(s) 499, 580, 662, 708
Asp700I GAANNNNTTC 2 cut(s) 630, 633
AspLEI GCGC 1 cut(s) 13
AspS9I GGNCC 2 cut(s) 723, 724
AsuC2I CCSGG 1 cut(s) 67
BaeGI GKGCMC 1 cut(s) 727
BalI TGGCCA 1 cut(s) 235
BanII GRGCYC 1 cut(s) 727
BbvI GCAGC 1 cut(s) 488
BciT130I CCWGG 1 cut(s) 237
BcnI CCSGG 1 cut(s) 67
BcoDI GTCTC 2 cut(s) 83, 168
BcuI ACTAGT 1 cut(s) 389
BfaI CTAG 6 cut(s) 56, 390, 488, 557, 671, 705
BfmI CTRYAG 1 cut(s) 809
BfoI RGCGCY 1 cut(s) 14
BglII AGATCT 2 cut(s) 411, 510
BisI GCNGC 2 cut(s) 9, 477
BlsI GCNGC 2 cut(s) 10, 478
Bme1390I CCNGG 2 cut(s) 67, 237
BmgT120I GGNCC 2 cut(s) 723, 724
BmiI GGNNCC 1 cut(s) 725
BmrFI CCNGG 2 cut(s) 67, 237
BmsI GCATC 2 cut(s) 281, 726
BplI GAGNNNNNCTC 2 cut(s) 497, 529
BpuEI CTTGAG 1 cut(s) 150
BpuMI CCSGG 1 cut(s) 67
BsaBI GATNNNNATC 1 cut(s) 210
BsaI GGTCTC 1 cut(s) 168
BsaJI CCNNGG 2 cut(s) 65, 236
Bsc4I CCNNNNNNNGG 2 cut(s) 66, 71
Bse1I ACTGG 1 cut(s) 231
Bse3DI GCAATG 2 cut(s) 249, 345
Bse8I GATNNNNATC 1 cut(s) 210
BseBI CCWGG 1 cut(s) 237
BseDI CCNNGG 2 cut(s) 65, 236
BseGI GGATG 1 cut(s) 296
BseJI GATNNNNATC 1 cut(s) 210
BseLI CCNNNNNNNGG 2 cut(s) 66, 71
BseMI GCAATG 2 cut(s) 249, 345
BseMII CTCAG 2 cut(s) 422, 495
BseNI ACTGG 1 cut(s) 231
BseSI GKGCMC 1 cut(s) 727
BseXI GCAGC 1 cut(s) 488
Bsh1285I CGRYCG 1 cut(s) 71
BshFI GGCC 2 cut(s) 235, 725
BsiEI CGRYCG 1 cut(s) 71
BsiSI CCGG 1 cut(s) 67
BslFI GGGAC 2 cut(s) 667, 772
BslI CCNNNNNNNGG 2 cut(s) 66, 71
BsmAI GTCTC 2 cut(s) 83, 168
BsmBI CGTCTC 1 cut(s) 83
BsmFI GGGAC 2 cut(s) 667, 772
BsmI GAATGC 2 cut(s) 277, 359
BsnI GGCC 2 cut(s) 235, 725
Bso31I GGTCTC 1 cut(s) 168
Bsp120I GGGCCC 1 cut(s) 723
Bsp1286I GDGCHC 1 cut(s) 727
Bsp143I GATC 5 cut(s) 211, 279, 411, 510, 764
BspACI CCGC 2 cut(s) 8, 721
BspANI GGCC 2 cut(s) 235, 725
BspCNI CTCAG 2 cut(s) 421, 496
BspLI GGNNCC 1 cut(s) 725
BspPI GGATC 2 cut(s) 274, 759
BspTNI GGTCTC 1 cut(s) 168
BsrDI GCAATG 2 cut(s) 249, 345
BsrI ACTGG 1 cut(s) 231
BssECI CCNNGG 2 cut(s) 65, 236
BssMI GATC 5 cut(s) 211, 279, 411, 510, 764
Bst2UI CCWGG 1 cut(s) 237
Bst4CI ACNGT 5 cut(s) 29, 94, 464, 594, 622
BstC8I GCNNGC 1 cut(s) 723
BstDEI CTNAG 4 cut(s) 267, 408, 504, 690
BstF5I GGATG 1 cut(s) 296
BstH2I RGCGCY 1 cut(s) 14
BstHHI GCGC 1 cut(s) 13
BstKTI GATC 5 cut(s) 214, 282, 414, 513, 767
BstMAI GTCTC 2 cut(s) 83, 168
BstMBI GATC 5 cut(s) 211, 279, 411, 510, 764
BstMCI CGRYCG 1 cut(s) 71
BstMWI GCNNNNNNNGC 1 cut(s) 473
BstNI CCWGG 1 cut(s) 237
BstSCI CCNGG 2 cut(s) 65, 235
BstSFI CTRYAG 1 cut(s) 809
BstSLI GKGCMC 1 cut(s) 727
BstV1I GCAGC 1 cut(s) 488
BstX2I RGATCY 3 cut(s) 279, 411, 510
BstYI RGATCY 3 cut(s) 279, 411, 510
BsuRI GGCC 2 cut(s) 235, 725
BtsCI GGATG 1 cut(s) 296
BtsIMutI CAGTG 2 cut(s) 201, 627
Cac8I GCNNGC 1 cut(s) 723
CfoI GCGC 1 cut(s) 13
Cfr13I GGNCC 2 cut(s) 723, 724
Csp6I GTAC 1 cut(s) 23
CspCI CAANNNNNGTGG 4 cut(s) 190, 212, 225, 247
CviJI RGCY 8 cut(s) 59, 83, 142, 235, 433, 563, 674, 725
CviKI_1 RGCY 8 cut(s) 59, 83, 142, 235, 433, 563, 674, 725
CviQI GTAC 1 cut(s) 23
DdeI CTNAG 4 cut(s) 267, 408, 504, 690
DpnI GATC 5 cut(s) 213, 281, 413, 512, 766
DpnII GATC 5 cut(s) 211, 279, 411, 510, 764
EaeI YGGCCR 1 cut(s) 233
Eco24I GRGCYC 1 cut(s) 727
Eco31I GGTCTC 1 cut(s) 168
EcoRI GAATTC 2 cut(s) 580, 708
EcoRII CCWGG 1 cut(s) 235
EcoT38I GRGCYC 1 cut(s) 727
Esp3I CGTCTC 1 cut(s) 83
FalI AAGNNNNNCTT 4 cut(s) 589, 621, 618, 650
FaqI GGGAC 2 cut(s) 667, 772
FauI CCCGC 1 cut(s) 714
Fnu4HI GCNGC 2 cut(s) 9, 477
FokI GGATG 1 cut(s) 303
FriOI GRGCYC 1 cut(s) 727
Fsp4HI GCNGC 2 cut(s) 9, 477
FspBI CTAG 6 cut(s) 56, 390, 488, 557, 671, 705
GlaI GCGC 1 cut(s) 12
GluI GCNGC 2 cut(s) 9, 477
HaeII RGCGCY 1 cut(s) 14
HaeIII GGCC 2 cut(s) 235, 725
HapII CCGG 1 cut(s) 67
HhaI GCGC 1 cut(s) 13
Hin6I GCGC 1 cut(s) 11
HinP1I GCGC 1 cut(s) 11
HindIII AAGCTT 1 cut(s) 81
HinfI GANTC 2 cut(s) 553, 760
HpaII CCGG 1 cut(s) 67
Hpy166II GTNNAC 3 cut(s) 25, 115, 805
Hpy188I TCNGA 4 cut(s) 411, 448, 505, 700
Hpy188III TCNNGA 5 cut(s) 415, 455, 537, 577, 782
Hpy8I GTNNAC 3 cut(s) 25, 115, 805
Hpy99I CGWCG 1 cut(s) 732
HpyAV CCTTC 3 cut(s) 644, 778, 779
HpyCH4III ACNGT 5 cut(s) 29, 94, 464, 594, 622
HpyCH4IV ACGT 3 cut(s) 105, 117, 730
HpyCH4V TGCA 6 cut(s) 275, 286, 294, 368, 660, 739
HpyF10VI GCNNNNNNNGC 1 cut(s) 473
HpyF3I CTNAG 4 cut(s) 267, 408, 504, 690
HpySE526I ACGT 3 cut(s) 105, 117, 730
HspAI GCGC 1 cut(s) 11
Kzo9I GATC 5 cut(s) 211, 279, 411, 510, 764
LmnI GCTCC 1 cut(s) 560
Lsp1109I GCAGC 1 cut(s) 488
LweI GCATC 2 cut(s) 281, 726
MaeI CTAG 6 cut(s) 56, 390, 488, 557, 671, 705
MaeII ACGT 3 cut(s) 105, 117, 730
MaeIII GTNAC 2 cut(s) 74, 134
MalI GATC 5 cut(s) 213, 281, 413, 512, 766
MboI GATC 5 cut(s) 211, 279, 411, 510, 764
MboII GAAGA 5 cut(s) 164, 339, 435, 626, 638
MflI RGATCY 3 cut(s) 279, 411, 510
MhlI GDGCHC 1 cut(s) 727
MlsI TGGCCA 1 cut(s) 235
MluCI AATT 6 cut(s) 361, 499, 580, 662, 708, 749
MluNI TGGCCA 1 cut(s) 235
MlyI GAGTC 1 cut(s) 754
MnlI CCTC 5 cut(s) 63, 70, 197, 533, 793
Mox20I TGGCCA 1 cut(s) 235
MroXI GAANNNNTTC 2 cut(s) 630, 633
MscI TGGCCA 1 cut(s) 235
MseI TTAA 1 cut(s) 395
Msp20I TGGCCA 1 cut(s) 235
MspA1I CMGCKG 1 cut(s) 142
MspI CCGG 1 cut(s) 67
MspR9I CCNGG 2 cut(s) 67, 237
Mva1269I GAATGC 2 cut(s) 277, 359
MvaI CCWGG 1 cut(s) 237
MwoI GCNNNNNNNGC 1 cut(s) 473
NciI CCSGG 1 cut(s) 67
NdeII GATC 5 cut(s) 211, 279, 411, 510, 764
NlaIV GGNNCC 1 cut(s) 725
PctI GAATGC 2 cut(s) 277, 359
PdmI GAANNNNTTC 2 cut(s) 630, 633
PfeI GAWTC 1 cut(s) 553
PkrI GCNGC 2 cut(s) 10, 478
PleI GAGTC 1 cut(s) 754
PpsI GAGTC 1 cut(s) 754
Psp6I CCWGG 1 cut(s) 235
PspGI CCWGG 1 cut(s) 235
PspN4I GGNNCC 1 cut(s) 725
PspOMI GGGCCC 1 cut(s) 723
PspPI GGNCC 2 cut(s) 723, 724
PsuI RGATCY 3 cut(s) 279, 411, 510
PvuII CAGCTG 1 cut(s) 142
RsaI GTAC 1 cut(s) 24
RsaNI GTAC 1 cut(s) 23
SaqAI TTAA 1 cut(s) 395
SatI GCNGC 2 cut(s) 9, 477
Sau3AI GATC 5 cut(s) 211, 279, 411, 510, 764
Sau96I GGNCC 2 cut(s) 723, 724
SchI GAGTC 1 cut(s) 754
ScrFI CCNGG 2 cut(s) 67, 237
SduI GDGCHC 1 cut(s) 727
SfaNI GCATC 2 cut(s) 281, 726
SfcI CTRYAG 1 cut(s) 809
SmlI CTYRAG 1 cut(s) 165
SmoI CTYRAG 1 cut(s) 165
SpeI ACTAGT 1 cut(s) 389
Sse9I AATT 6 cut(s) 361, 499, 580, 662, 708, 749
SsiI CCGC 2 cut(s) 8, 721
SspMI CTAG 6 cut(s) 56, 390, 488, 557, 671, 705
StyD4I CCNGG 2 cut(s) 65, 235
TaaI ACNGT 5 cut(s) 29, 94, 464, 594, 622
TaiI ACGT 3 cut(s) 108, 120, 733
TaqI TCGA 6 cut(s) 46, 109, 546, 637, 763, 783
TasI AATT 6 cut(s) 361, 499, 580, 662, 708, 749
TauI GCSGC 1 cut(s) 11
TfiI GAWTC 1 cut(s) 553
Tru1I TTAA 1 cut(s) 395
Tru9I TTAA 1 cut(s) 395
TscAI CASTG 2 cut(s) 208, 627
TseI GCWGC 1 cut(s) 476
TspDTI ATGAA 3 cut(s) 655, 731, 762
TspRI CASTG 2 cut(s) 208, 627
XapI RAATTY 4 cut(s) 499, 580, 662, 708
XmnI GAANNNNTTC 2 cut(s) 630, 633
XspI CTAG 6 cut(s) 56, 390, 488, 557, 671, 705
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.