Rw5G031300

Lysine methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0004944
Physical Location & Seq
Reverse (-)
51175077 .. 51183684
8608 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G031300.1

Sequence Viewer

Length: 720 bp
ATGGAAAGAGAAGGAGAAGACGACGACGACATGGTTTGCTTAGACGAATCGTTTTTCATCGATGACAATTACCAGCTGACCACTTTCACATTTGGGTCTCAAGTTGTTCAACTCCTTTGCCTCCAATCTGCTTCCACTGATTTTGATCTTACAGGACAATTGGTGTGGCCAGGGGCAATGCTGTTGAATGATTACCTTTCTAAGAATGCAGAGATCCTGCAAGGATGCACTGTTATTGAGTTAGGGTCTGGGGTTGGTATTACTGGAATACTATGCAGCAGATTTTGCAGTCAAGTTGTTTTAACAGACCACAATGAAGAAGTGCTCAAGATCTTGAATAAAAATATAGAGCTACACGCATCTTCTGATAATCCTGAATACTGTGCTGGATTAGCAGCGGAGAAACTAGAATGGGGAAATTCTGAGCAGATCTCCCAGATTTTACAAAAGTATTCAAGAGGGTTCGATTTGATTCTTGGAGCTGATATATGCTTTCAGCAGTCAAGCATTACTTCGCTTTTTGACACGGTGGAAGAACTTCTTCGAGTTAGAAAAGAAGGAAAGTGCAAATTCATACTAGCCTATGTGTCCCGTGCTAAGACCATGGATTCATTGGTCATTAGTGAAGCGGCCCGACGTAAGATGCAGATAAATGAATTAGTTGGGACTCGATCCTTCGTTGGCAATCTCGAAGGACTTATCTATGAGGTGATCCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_16

Protein Analysis

239

Amino Acids

26.65

Weight (kDa)

4.43

Isoelectric Point (pI)

49.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_16 PF10294 51 - 200 6.9e-22 Lysine methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 398, 629
AclWI GGATC 3 cut(s) 208, 666, 706
AcoI YGGCCR 1 cut(s) 167
AcsI RAATTY 2 cut(s) 418, 569
AgsI TTSAA 4 cut(s) 110, 187, 337, 456
AjnI CCWGG 1 cut(s) 169
AluBI AGCT 3 cut(s) 76, 352, 482
AluI AGCT 3 cut(s) 76, 352, 482
Alw21I GWGCWC 1 cut(s) 327
Alw26I GTCTC 1 cut(s) 102
AlwI GGATC 3 cut(s) 208, 666, 706
AoxI GGCC 2 cut(s) 167, 630
ApeKI GCWGC 2 cut(s) 276, 395
ApoI RAATTY 2 cut(s) 418, 569
Asp700I GAANNNNTTC 2 cut(s) 537, 540
AspS9I GGNCC 1 cut(s) 631
BalI TGGCCA 1 cut(s) 169
BbsI GAAGAC 1 cut(s) 24
Bbv12I GWGCWC 1 cut(s) 327
BbvI GCAGC 2 cut(s) 288, 407
BciT130I CCWGG 1 cut(s) 171
BcoDI GTCTC 1 cut(s) 102
BfaI CTAG 2 cut(s) 407, 578
BglII AGATCT 2 cut(s) 330, 429
BisI GCNGC 3 cut(s) 277, 396, 630
BlsI GCNGC 3 cut(s) 278, 397, 631
Bme1390I CCNGG 1 cut(s) 171
BmgT120I GGNCC 1 cut(s) 631
BmrFI CCNGG 1 cut(s) 171
BmsI GCATC 3 cut(s) 215, 368, 633
BpiI GAAGAC 1 cut(s) 24
BplI GAGNNNNNCTC 2 cut(s) 416, 448
BpuEI CTTGAG 2 cut(s) 84, 311
Bsa29I ATCGAT 1 cut(s) 60
BsaBI GATNNNNATC 1 cut(s) 144
BsaI GGTCTC 1 cut(s) 102
BsaJI CCNNGG 2 cut(s) 170, 603
Bse1I ACTGG 1 cut(s) 268
Bse3DI GCAATG 1 cut(s) 183
Bse8I GATNNNNATC 1 cut(s) 144
BseBI CCWGG 1 cut(s) 171
BseCI ATCGAT 1 cut(s) 60
BseDI CCNNGG 2 cut(s) 170, 603
BseGI GGATG 1 cut(s) 230
BseJI GATNNNNATC 1 cut(s) 144
BseMI GCAATG 1 cut(s) 183
BseMII CTCAG 1 cut(s) 414
BseNI ACTGG 1 cut(s) 268
BseXI GCAGC 2 cut(s) 288, 407
BshFI GGCC 2 cut(s) 169, 632
BshVI ATCGAT 1 cut(s) 60
BsiHKAI GWGCWC 1 cut(s) 327
BslFI GGGAC 2 cut(s) 574, 679
BsmAI GTCTC 1 cut(s) 102
BsmFI GGGAC 2 cut(s) 574, 679
BsmI GAATGC 1 cut(s) 211
BsnI GGCC 2 cut(s) 169, 632
Bso31I GGTCTC 1 cut(s) 102
Bsp1286I GDGCHC 1 cut(s) 327
Bsp143I GATC 6 cut(s) 145, 213, 330, 429, 671, 711
Bsp19I CCATGG 1 cut(s) 603
BspACI CCGC 2 cut(s) 398, 629
BspANI GGCC 2 cut(s) 169, 632
BspCNI CTCAG 1 cut(s) 415
BspDI ATCGAT 1 cut(s) 60
BspPI GGATC 3 cut(s) 208, 666, 706
BspTNI GGTCTC 1 cut(s) 102
BsrDI GCAATG 1 cut(s) 183
BsrI ACTGG 1 cut(s) 268
BssECI CCNNGG 2 cut(s) 170, 603
BssMI GATC 6 cut(s) 145, 213, 330, 429, 671, 711
BssT1I CCWWGG 1 cut(s) 603
Bst2UI CCWGG 1 cut(s) 171
Bst4CI ACNGT 3 cut(s) 232, 383, 529
BstAPI GCANNNNNTGC 1 cut(s) 285
BstDEI CTNAG 4 cut(s) 40, 201, 423, 597
BstDSI CCRYGG 1 cut(s) 603
BstF5I GGATG 1 cut(s) 230
BstKTI GATC 6 cut(s) 148, 216, 333, 432, 674, 714
BstMAI GTCTC 1 cut(s) 102
BstMBI GATC 6 cut(s) 145, 213, 330, 429, 671, 711
BstMWI GCNNNNNNNGC 2 cut(s) 285, 392
BstNI CCWGG 1 cut(s) 171
BstSCI CCNGG 1 cut(s) 169
BstV1I GCAGC 2 cut(s) 288, 407
BstV2I GAAGAC 1 cut(s) 24
BstX2I RGATCY 3 cut(s) 213, 330, 429
BstYI RGATCY 3 cut(s) 213, 330, 429
Bsu15I ATCGAT 1 cut(s) 60
BsuRI GGCC 2 cut(s) 169, 632
BsuTUI ATCGAT 1 cut(s) 60
BtgI CCRYGG 1 cut(s) 603
BtsCI GGATG 1 cut(s) 230
BtsIMutI CAGTG 2 cut(s) 135, 228
Cfr13I GGNCC 1 cut(s) 631
ClaI ATCGAT 1 cut(s) 60
CspCI CAANNNNNGTGG 4 cut(s) 124, 146, 159, 181
CviAII CATG 2 cut(s) 31, 604
CviJI RGCY 6 cut(s) 76, 169, 352, 482, 581, 632
CviKI_1 RGCY 6 cut(s) 76, 169, 352, 482, 581, 632
DdeI CTNAG 4 cut(s) 40, 201, 423, 597
DpnI GATC 6 cut(s) 147, 215, 332, 431, 673, 713
DpnII GATC 6 cut(s) 145, 213, 330, 429, 671, 711
EaeI YGGCCR 1 cut(s) 167
Eco130I CCWWGG 1 cut(s) 603
Eco31I GGTCTC 1 cut(s) 102
EcoRII CCWGG 1 cut(s) 169
EcoT14I CCWWGG 1 cut(s) 603
ErhI CCWWGG 1 cut(s) 603
FaeI CATG 2 cut(s) 34, 607
FaiI YATR 9 cut(s) 32, 274, 347, 488, 490, 575, 585, 605, 705
FalI AAGNNNNNCTT 4 cut(s) 496, 528, 525, 557
FaqI GGGAC 2 cut(s) 574, 679
FatI CATG 2 cut(s) 30, 603
Fnu4HI GCNGC 3 cut(s) 277, 396, 630
FokI GGATG 1 cut(s) 237
Fsp4HI GCNGC 3 cut(s) 277, 396, 630
FspBI CTAG 2 cut(s) 407, 578
GluI GCNGC 3 cut(s) 277, 396, 630
HaeIII GGCC 2 cut(s) 169, 632
Hin1II CATG 2 cut(s) 34, 607
HinfI GANTC 4 cut(s) 47, 472, 608, 667
Hpy188I TCNGA 2 cut(s) 367, 424
Hpy188III TCNNGA 5 cut(s) 328, 334, 374, 456, 689
Hpy99I CGWCG 3 cut(s) 26, 29, 639
HpyAV CCTTC 4 cut(s) 5, 551, 685, 686
HpyCH4III ACNGT 3 cut(s) 232, 383, 529
HpyCH4IV ACGT 1 cut(s) 637
HpyCH4V TGCA 7 cut(s) 209, 220, 228, 276, 288, 567, 646
HpyF10VI GCNNNNNNNGC 2 cut(s) 285, 392
HpyF3I CTNAG 4 cut(s) 40, 201, 423, 597
HpySE526I ACGT 1 cut(s) 637
Hsp92II CATG 2 cut(s) 34, 607
Kzo9I GATC 6 cut(s) 145, 213, 330, 429, 671, 711
LmnI GCTCC 1 cut(s) 479
Lsp1109I GCAGC 2 cut(s) 288, 407
LweI GCATC 3 cut(s) 215, 368, 633
MaeI CTAG 2 cut(s) 407, 578
MaeII ACGT 1 cut(s) 637
MalI GATC 6 cut(s) 147, 215, 332, 431, 673, 713
MboI GATC 6 cut(s) 145, 213, 330, 429, 671, 711
MboII GAAGA 5 cut(s) 29, 329, 354, 533, 545
MfeI CAATTG 1 cut(s) 158
MflI RGATCY 3 cut(s) 213, 330, 429
MhlI GDGCHC 1 cut(s) 327
MlsI TGGCCA 1 cut(s) 169
MluCI AATT 5 cut(s) 67, 158, 418, 569, 656
MluNI TGGCCA 1 cut(s) 169
MlyI GAGTC 1 cut(s) 661
MnlI CCTC 3 cut(s) 131, 452, 700
Mox20I TGGCCA 1 cut(s) 169
MroXI GAANNNNTTC 2 cut(s) 537, 540
MscI TGGCCA 1 cut(s) 169
MseI TTAA 1 cut(s) 302
Msp20I TGGCCA 1 cut(s) 169
MspA1I CMGCKG 2 cut(s) 76, 398
MspR9I CCNGG 1 cut(s) 171
MunI CAATTG 1 cut(s) 158
Mva1269I GAATGC 1 cut(s) 211
MvaI CCWGG 1 cut(s) 171
MwoI GCNNNNNNNGC 2 cut(s) 285, 392
NcoI CCATGG 1 cut(s) 603
NdeII GATC 6 cut(s) 145, 213, 330, 429, 671, 711
NlaIII CATG 2 cut(s) 34, 607
PctI GAATGC 1 cut(s) 211
PdmI GAANNNNTTC 2 cut(s) 537, 540
PfeI GAWTC 3 cut(s) 47, 472, 608
PkrI GCNGC 3 cut(s) 278, 397, 631
PleI GAGTC 1 cut(s) 661
PpsI GAGTC 1 cut(s) 661
Psp6I CCWGG 1 cut(s) 169
PspGI CCWGG 1 cut(s) 169
PspPI GGNCC 1 cut(s) 631
PsuI RGATCY 3 cut(s) 213, 330, 429
PvuII CAGCTG 1 cut(s) 76
SaqAI TTAA 1 cut(s) 302
SatI GCNGC 3 cut(s) 277, 396, 630
Sau3AI GATC 6 cut(s) 145, 213, 330, 429, 671, 711
Sau96I GGNCC 1 cut(s) 631
SchI GAGTC 1 cut(s) 661
ScrFI CCNGG 1 cut(s) 171
SduI GDGCHC 1 cut(s) 327
SetI ASST 6 cut(s) 78, 198, 354, 484, 640, 711
SfaNI GCATC 3 cut(s) 215, 368, 633
SmlI CTYRAG 2 cut(s) 99, 326
SmoI CTYRAG 2 cut(s) 99, 326
Sse9I AATT 5 cut(s) 67, 158, 418, 569, 656
SsiI CCGC 2 cut(s) 398, 629
SspMI CTAG 2 cut(s) 407, 578
StyD4I CCNGG 1 cut(s) 169
StyI CCWWGG 1 cut(s) 603
TaaI ACNGT 3 cut(s) 232, 383, 529
TaiI ACGT 1 cut(s) 640
TaqI TCGA 5 cut(s) 60, 465, 544, 670, 690
TasI AATT 5 cut(s) 67, 158, 418, 569, 656
TauI GCSGC 1 cut(s) 632
TfiI GAWTC 3 cut(s) 47, 472, 608
Tru1I TTAA 1 cut(s) 302
Tru9I TTAA 1 cut(s) 302
TscAI CASTG 2 cut(s) 142, 235
TseI GCWGC 2 cut(s) 276, 395
TspDTI ATGAA 5 cut(s) 46, 330, 562, 600, 669
TspRI CASTG 2 cut(s) 142, 235
XapI RAATTY 2 cut(s) 418, 569
XcmI CCANNNNNNNNNTGG 1 cut(s) 610
XmnI GAANNNNTTC 2 cut(s) 537, 540
XspI CTAG 2 cut(s) 407, 578
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.