Rh5AG331200

Lysine methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0004944
Physical Location & Seq
Reverse (-)
52708678 .. 52717551
8874 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG331200.1

Sequence Viewer

Length: 582 bp
ATGGAAAGAGAAGGAGAAGACGACGACATAGTCTGCTTAGACGAATCGTTTTTCATCGATGACAATTACCAGCTGACCACTTTCACTTTTGGGTCTCAAGTTCTTCAACTCCTTTGCCTCCAATCTGCTTCCACTGATTTTGATCTTACAGGACAATTGGTGTGGCCAGGGGCAATGCTGTTGAATGATTACCTTTCTAAGAATGCAGAGATCCTGCAAGGATGCACTGTTATTGAGTTAGGGTCTGGGGTTGGTATTACTGGAATACTATGCAGCAGATTTTGCAATCAAGTTGTTTTAACAGACCACAATGAAGAAGTGCTCAAGATCTTGAATAAAAATATAGAGCTACACGCATCTTCTGATAATCCTGAATACTGTGCTGGATTAGCAGCGGAGAAACTAGAATGGGGAAATTCTGAGCAGATCTCCCAGATTTTACAAAAGTATTCAAGAGGGTTCGATTTGATTCTTGGAGCTGATATATATATCCTGAATTGTTATTCTTTTGCCTTTATAATAAAGAAAGTTGGGATCTATTCAGGGATCCCACAAGTTGACCAAAAAAATGAAAATAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_16

Protein Analysis

193

Amino Acids

21.5

Weight (kDa)

4.19

Isoelectric Point (pI)

40.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_16 PF10294 50 - 163 9.9e-17 Lysine methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 518
AasI GACNNNNNNGTC 1 cut(s) 29
AciI CCGC 1 cut(s) 395
AclWI GGATC 4 cut(s) 205, 541, 542, 554
AcoI YGGCCR 1 cut(s) 164
AcsI RAATTY 1 cut(s) 415
AgsI TTSAA 4 cut(s) 107, 184, 334, 453
AjnI CCWGG 1 cut(s) 166
AluBI AGCT 3 cut(s) 73, 349, 479
AluI AGCT 3 cut(s) 73, 349, 479
Alw21I GWGCWC 1 cut(s) 324
Alw26I GTCTC 1 cut(s) 99
AlwI GGATC 4 cut(s) 205, 541, 542, 554
AoxI GGCC 1 cut(s) 164
ApeKI GCWGC 2 cut(s) 273, 392
ApoI RAATTY 1 cut(s) 415
BalI TGGCCA 1 cut(s) 166
BamHI GGATCC 1 cut(s) 546
BbsI GAAGAC 1 cut(s) 24
Bbv12I GWGCWC 1 cut(s) 324
BbvI GCAGC 2 cut(s) 285, 404
BciT130I CCWGG 1 cut(s) 168
BcoDI GTCTC 1 cut(s) 99
BfaI CTAG 1 cut(s) 404
BglII AGATCT 2 cut(s) 327, 426
BisI GCNGC 2 cut(s) 274, 393
BlsI GCNGC 2 cut(s) 275, 394
Bme1390I CCNGG 1 cut(s) 168
BmiI GGNNCC 1 cut(s) 548
BmrFI CCNGG 1 cut(s) 168
BmsI GCATC 2 cut(s) 212, 365
BpiI GAAGAC 1 cut(s) 24
BplI GAGNNNNNCTC 2 cut(s) 413, 445
BpuEI CTTGAG 2 cut(s) 81, 308
Bsa29I ATCGAT 1 cut(s) 57
BsaBI GATNNNNATC 1 cut(s) 141
BsaI GGTCTC 1 cut(s) 99
BsaJI CCNNGG 1 cut(s) 167
Bse1I ACTGG 1 cut(s) 265
Bse3DI GCAATG 1 cut(s) 180
Bse8I GATNNNNATC 1 cut(s) 141
BseBI CCWGG 1 cut(s) 168
BseCI ATCGAT 1 cut(s) 57
BseDI CCNNGG 1 cut(s) 167
BseGI GGATG 1 cut(s) 227
BseJI GATNNNNATC 1 cut(s) 141
BseMI GCAATG 1 cut(s) 180
BseMII CTCAG 1 cut(s) 411
BseNI ACTGG 1 cut(s) 265
BseXI GCAGC 2 cut(s) 285, 404
BshFI GGCC 1 cut(s) 166
BshVI ATCGAT 1 cut(s) 57
BsiHKAI GWGCWC 1 cut(s) 324
BsmAI GTCTC 1 cut(s) 99
BsmI GAATGC 1 cut(s) 208
BsnI GGCC 1 cut(s) 166
Bso31I GGTCTC 1 cut(s) 99
Bsp1286I GDGCHC 1 cut(s) 324
Bsp143I GATC 6 cut(s) 142, 210, 327, 426, 534, 546
BspACI CCGC 1 cut(s) 395
BspANI GGCC 1 cut(s) 166
BspCNI CTCAG 1 cut(s) 412
BspDI ATCGAT 1 cut(s) 57
BspLI GGNNCC 1 cut(s) 548
BspPI GGATC 4 cut(s) 205, 541, 542, 554
BspTNI GGTCTC 1 cut(s) 99
BsrDI GCAATG 1 cut(s) 180
BsrI ACTGG 1 cut(s) 265
BssECI CCNNGG 1 cut(s) 167
BssMI GATC 6 cut(s) 142, 210, 327, 426, 534, 546
Bst2UI CCWGG 1 cut(s) 168
Bst4CI ACNGT 2 cut(s) 229, 380
BstAPI GCANNNNNTGC 1 cut(s) 282
BstDEI CTNAG 3 cut(s) 37, 198, 420
BstF5I GGATG 1 cut(s) 227
BstKTI GATC 6 cut(s) 145, 213, 330, 429, 537, 549
BstMAI GTCTC 1 cut(s) 99
BstMBI GATC 6 cut(s) 142, 210, 327, 426, 534, 546
BstMWI GCNNNNNNNGC 2 cut(s) 282, 389
BstNI CCWGG 1 cut(s) 168
BstSCI CCNGG 1 cut(s) 166
BstV1I GCAGC 2 cut(s) 285, 404
BstV2I GAAGAC 1 cut(s) 24
BstX2I RGATCY 5 cut(s) 210, 327, 426, 534, 546
BstYI RGATCY 5 cut(s) 210, 327, 426, 534, 546
Bsu15I ATCGAT 1 cut(s) 57
BsuRI GGCC 1 cut(s) 166
BsuTUI ATCGAT 1 cut(s) 57
BtsCI GGATG 1 cut(s) 227
BtsIMutI CAGTG 2 cut(s) 132, 225
ClaI ATCGAT 1 cut(s) 57
CspCI CAANNNNNGTGG 4 cut(s) 121, 143, 156, 178
CviJI RGCY 4 cut(s) 73, 166, 349, 479
CviKI_1 RGCY 4 cut(s) 73, 166, 349, 479
DdeI CTNAG 3 cut(s) 37, 198, 420
DpnI GATC 6 cut(s) 144, 212, 329, 428, 536, 548
DpnII GATC 6 cut(s) 142, 210, 327, 426, 534, 546
DrdI GACNNNNNNGTC 1 cut(s) 29
DseDI GACNNNNNNGTC 1 cut(s) 29
EaeI YGGCCR 1 cut(s) 164
Eco31I GGTCTC 1 cut(s) 99
EcoRII CCWGG 1 cut(s) 166
FaiI YATR 7 cut(s) 29, 271, 344, 485, 487, 489, 518
Fnu4HI GCNGC 2 cut(s) 274, 393
FokI GGATG 1 cut(s) 234
Fsp4HI GCNGC 2 cut(s) 274, 393
FspBI CTAG 1 cut(s) 404
GluI GCNGC 2 cut(s) 274, 393
HaeIII GGCC 1 cut(s) 166
HincII GTYRAC 1 cut(s) 559
HindII GTYRAC 1 cut(s) 559
HinfI GANTC 2 cut(s) 44, 469
Hpy166II GTNNAC 1 cut(s) 559
Hpy188I TCNGA 2 cut(s) 364, 421
Hpy188III TCNNGA 5 cut(s) 325, 331, 371, 453, 493
Hpy8I GTNNAC 1 cut(s) 559
Hpy99I CGWCG 1 cut(s) 26
HpyAV CCTTC 1 cut(s) 5
HpyCH4III ACNGT 2 cut(s) 229, 380
HpyCH4V TGCA 5 cut(s) 206, 217, 225, 273, 285
HpyF10VI GCNNNNNNNGC 2 cut(s) 282, 389
HpyF3I CTNAG 3 cut(s) 37, 198, 420
Kzo9I GATC 6 cut(s) 142, 210, 327, 426, 534, 546
LmnI GCTCC 1 cut(s) 476
Lsp1109I GCAGC 2 cut(s) 285, 404
LweI GCATC 2 cut(s) 212, 365
MaeI CTAG 1 cut(s) 404
MalI GATC 6 cut(s) 144, 212, 329, 428, 536, 548
MboI GATC 6 cut(s) 142, 210, 327, 426, 534, 546
MboII GAAGA 4 cut(s) 29, 95, 326, 351
MfeI CAATTG 1 cut(s) 155
MflI RGATCY 5 cut(s) 210, 327, 426, 534, 546
MhlI GDGCHC 1 cut(s) 324
MlsI TGGCCA 1 cut(s) 166
MluCI AATT 5 cut(s) 64, 155, 415, 496, 577
MluNI TGGCCA 1 cut(s) 166
MnlI CCTC 2 cut(s) 128, 449
Mox20I TGGCCA 1 cut(s) 166
MscI TGGCCA 1 cut(s) 166
MseI TTAA 1 cut(s) 299
Msp20I TGGCCA 1 cut(s) 166
MspA1I CMGCKG 2 cut(s) 73, 395
MspR9I CCNGG 1 cut(s) 168
MunI CAATTG 1 cut(s) 155
Mva1269I GAATGC 1 cut(s) 208
MvaI CCWGG 1 cut(s) 168
MwoI GCNNNNNNNGC 2 cut(s) 282, 389
NdeII GATC 6 cut(s) 142, 210, 327, 426, 534, 546
NlaIV GGNNCC 1 cut(s) 548
PctI GAATGC 1 cut(s) 208
PfeI GAWTC 2 cut(s) 44, 469
PflFI GACNNNGTC 1 cut(s) 29
PkrI GCNGC 2 cut(s) 275, 394
PsiI TTATAA 1 cut(s) 518
Psp6I CCWGG 1 cut(s) 166
PspGI CCWGG 1 cut(s) 166
PspN4I GGNNCC 1 cut(s) 548
PsuI RGATCY 5 cut(s) 210, 327, 426, 534, 546
PsyI GACNNNGTC 1 cut(s) 29
PvuII CAGCTG 1 cut(s) 73
SaqAI TTAA 1 cut(s) 299
SatI GCNGC 2 cut(s) 274, 393
Sau3AI GATC 6 cut(s) 142, 210, 327, 426, 534, 546
ScrFI CCNGG 1 cut(s) 168
SduI GDGCHC 1 cut(s) 324
SetI ASST 4 cut(s) 75, 195, 351, 481
SfaNI GCATC 2 cut(s) 212, 365
SmlI CTYRAG 2 cut(s) 96, 323
SmoI CTYRAG 2 cut(s) 96, 323
Sse9I AATT 5 cut(s) 64, 155, 415, 496, 577
SsiI CCGC 1 cut(s) 395
SspMI CTAG 1 cut(s) 404
StyD4I CCNGG 1 cut(s) 166
TaaI ACNGT 2 cut(s) 229, 380
TaqI TCGA 2 cut(s) 57, 462
TasI AATT 5 cut(s) 64, 155, 415, 496, 577
TfiI GAWTC 2 cut(s) 44, 469
Tru1I TTAA 1 cut(s) 299
Tru9I TTAA 1 cut(s) 299
TscAI CASTG 2 cut(s) 139, 232
TseI GCWGC 2 cut(s) 273, 392
TspDTI ATGAA 2 cut(s) 43, 327
TspRI CASTG 2 cut(s) 139, 232
Tth111I GACNNNGTC 1 cut(s) 29
XapI RAATTY 1 cut(s) 415
XspI CTAG 1 cut(s) 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.