Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0004294

Physical Location & Seq

Genomic Coordinates Reverse (-)

14865146 .. 14881136

Gene Structure 15991 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00093510.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1506 bp

ATGACACTCTGTATTTTGTCATTTAAGCACACCCACAAGGATGATAGCTGGGATAAGAAATCTCGAGCATCTTTCAATGACTGGGATCTCCACATCTGGCTCAGTGAACTAGGTTCAGTTGACAGTGATGGTCGTAAGACGAGTACTGGTAATATTCCTTCCACAGTTAATTTAGATTCTAGAAAAACTGGGTCTTCTGTGAAGTTTTGGACCATGGCTGAAGCCTCCCCACCTCAGAAAATGGGCATTCAAAGAGTTGAGGTGGGGGAGAGAGGCCTTGTTGCTTTGAAGAATATAAGAAAGGGAGAGAAGCTGCTGTTTGTGCCTCCCTCTTTGTTCATCACTGCAGACTCAGAATGGACAAATGGGGAGGCTGGTAAGGTGTTGAAGCAAAATTCTGTACCAGACTGGCCATTACTAGCAACCTATCTGATTAGTGAAGCGAGTTACTTGAAATCTTCGAGATGGAACAACTATATATCTGCCTTACCAAGGCAGCCTTATTCACTTCTGTATTGGACTCGCGAAGAGCTTGATCGATACTTGGAGGCATCACAGATTAGGCAGAGGGCAATTGAAAGAGTTACTAATGTTGTTGGAACATACAATGATCTAAGGCTTAGGATATTCTCAAAGCACCCTGATTTATTTCCGGAAGAGGTATTCAACATAGAAACCTTCAAATGGTCATTTGGAATTCTTTTCTCACGCTTGGTTCGATTACCCTCAATGGATGGGAAGGTTGCCTTGGTTCCTTGGGCAGATATGCTGAATCATAGTTGTGAGGTGGATACGTTTTTGGATTATGATAAATCATCCCAGGGAGTTGTTTTTACAACAGATAGGCCATATCAGCCAGGTGAGCAGGTCTTTATTTCCTATGGGAAGAAGTCCAATGGAGAGTTGTTGCTATCATATGGGTTTGTCCCAAGCAAGGGCACCAACCCTAGTGATTCCGTTGAGTTGTCAGTTTCACTTCCAAAATCTGACAAATGTTATAAGGAGAAGTTGGAAGCGCTGAGAAAGTATGGACTATCAGCTACAGAGTGTTTTCCTGTACGAATTACTGGCTGGCCATCGGAGCTAATGGCATTTGCTTATTTAGCTGTCAGCCCTCCAAGTATGAACAAACAATTTGAAGAGCTAGCGGTGGACTTGTCTACTATGAAGAAAGCTTCTCCGAGTAGAGGAGTTTTGAGAGAGGATTTTGATCCGGTTGAAATTGCCCAAGCTTATGAAAATGTGCAAGCTAGCATTTGGATGTCATTAAGGAAATGGACCGGGTTCTTGGAATCGAGGGGGTTGAATTGTTATTTTCTCATAATCTTGGCTATGAAAGCGTGGCGAAAGTCGATTGTGAAACAGACAGCATGCCAAATAGAAGGTAGTGAGCTCTACATTGGGTTTTATCCGCAATATCAATTGGGGAAAAAACTAATAATAATAAGAGAAAGAAAAAAAATCTATGTAAATAGAGAGAATGGACTAGCCCCGCTAGTCAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00310 map00310

Pfam Domains

Protein Families

Rubis-subs-bind SET

Protein Analysis

501

Amino Acids

57.15

Weight (kDa)

8.98

Isoelectric Point (pI)

41.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SET	PF00856	91 - 295	7.5e-12	SET domain
Rubis-subs-bind	PF09273	327 - 386	5.7e-09	Rubisco LSMT substrate-binding
IGPS	PF00218	387 - 419	4.5e-06	Indole-3-glycerol phosphate synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004294)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G07670 AT3G07670
fragaria_vesca	FvH4_6g40490 FvH4_6g40490 FvH4_6g40490 FvH4_6g40490 FvH4_6g40490 FvH4_6g40490 FvH4_6g40490
malus_domestica	MD17G1118300.v1.1
prunus_persica	Prupe.3G196100_v2.0.a1 Prupe.3G196100_v2.0.a1
pyrus_communis	pycom17g10880
rosa_chinensis	RchiOBHm_Chr2g0155741 RchiOBHm_Chr2g0155751
rosa_laevigata	RLG00000020860
rosa_multiflora	Rmu_co8091832.1_g000001 Rmu_sc0002712.1_g000018 Rmu_sc0003417.1_g000003 Rmu_sc0011953.1_g000001
rosa_roxburghii	Rroxscaffold_2G00093510
rosa_rugosa	Rorug02G0449800
rosa_samantha	Rh2AG515700 Rh2AG515800 Rh2BG526700 Rh2BG526800 Rh2CG500700 Rh2CG500800 Rh2DG535700 Rh2DG535800
rosa_wichuraiana	Rw2G042410 Rw2G042420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	999
Acc36I	ACCTGC	1 cut(s)	856
AccB1I	GGYRCC	1 cut(s)	938
AccI	GTMKAC	1 cut(s)	1160
AccII	CGCG	1 cut(s)	525
AccIII	TCCGGA	1 cut(s)	652
AciI	CCGC	3 cut(s)	1148, 1412, 1493
AclWI	GGATC	2 cut(s)	93, 1205
AcoI	YGGCCR	2 cut(s)	410, 1073
AcsI	RAATTY	2 cut(s)	394, 696
AcuI	CTGAAG	1 cut(s)	240
AfaI	GTAC	3 cut(s)	145, 402, 1059
AfeI	AGCGCT	1 cut(s)	1017
AfiI	CCNNNNNNNGG	5 cut(s)	492, 684, 934, 935, 1187
AjnI	CCWGG	2 cut(s)	819, 856
AjuI	GAANNNNNNNTTGG	2 cut(s)	731, 763
Alw21I	GWGCWC	1 cut(s)	1395
AlwI	GGATC	2 cut(s)	93, 1205
Ama87I	CYCGRG	1 cut(s)	63
Aor13HI	TCCGGA	1 cut(s)	652
Aor51HI	AGCGCT	1 cut(s)	1017
AoxI	GGCC	4 cut(s)	274, 410, 845, 1073
ApeKI	GCWGC	2 cut(s)	313, 496
ApoI	RAATTY	2 cut(s)	394, 696
AspLEI	GCGC	1 cut(s)	1018
AspS9I	GGNCC	2 cut(s)	210, 1278
AsuC2I	CCSGG	1 cut(s)	1282
AsuHPI	GGTGA	1 cut(s)	872
AsuNHI	GCTAGC	2 cut(s)	1144, 1250
AvaI	CYCGRG	1 cut(s)	63
AvaII	GGWCC	2 cut(s)	210, 1278
BaeGI	GKGCMC	1 cut(s)	941
BalI	TGGCCA	2 cut(s)	412, 1075
BanI	GGYRCC	1 cut(s)	938
BanII	GRGCYC	1 cut(s)	1395
BarI	GAAGNNNNNNTAC	2 cut(s)	142, 174
BbsI	GAAGAC	1 cut(s)	186
Bbv12I	GWGCWC	1 cut(s)	1395
BbvI	GCAGC	2 cut(s)	300, 508
BccI	CCATC	4 cut(s)	122, 459, 728, 1084
BciT130I	CCWGG	2 cut(s)	821, 858
BciVI	GTATCC	1 cut(s)	784
BcnI	CCSGG	1 cut(s)	1282
BfaI	CTAG	8 cut(s)	110, 180, 419, 948, 1145, 1251, 1487, 1496
BfmI	CTRYAG	2 cut(s)	345, 1041
BfoI	RGCGCY	1 cut(s)	1019
BfuAI	ACCTGC	1 cut(s)	856
BfuI	GTATCC	1 cut(s)	784
BisI	GCNGC	2 cut(s)	314, 497
BlsI	GCNGC	2 cut(s)	315, 498
BmcAI	AGTACT	1 cut(s)	145
Bme1390I	CCNGG	3 cut(s)	821, 858, 1282
Bme18I	GGWCC	2 cut(s)	210, 1278
BmeT110I	CYCGRG	1 cut(s)	63
BmgT120I	GGNCC	2 cut(s)	210, 1278
BmiI	GGNNCC	2 cut(s)	753, 940
BmrFI	CCNGG	3 cut(s)	821, 858, 1282
BmrI	ACTGGG	2 cut(s)	91, 198
BmsI	GCATC	2 cut(s)	77, 560
BmtI	GCTAGC	2 cut(s)	1148, 1254
BmuI	ACTGGG	2 cut(s)	91, 198
BpiI	GAAGAC	1 cut(s)	186
Bpu10I	CCTNAGC	1 cut(s)	620
BpuMI	CCSGG	1 cut(s)	1282
Bsa29I	ATCGAT	1 cut(s)	538
BsaBI	GATNNNNATC	1 cut(s)	1209
BsaJI	CCNNGG	6 cut(s)	213, 491, 747, 755, 819, 820
BsaWI	WCCGGW	2 cut(s)	652, 1213
Bsc4I	CCNNNNNNNGG	5 cut(s)	492, 684, 934, 935, 1187
Bse1I	ACTGG	5 cut(s)	86, 151, 193, 413, 1072
Bse8I	GATNNNNATC	1 cut(s)	1209
BseAI	TCCGGA	1 cut(s)	652
BseBI	CCWGG	2 cut(s)	821, 858
BseCI	ATCGAT	1 cut(s)	538
BseDI	CCNNGG	6 cut(s)	213, 491, 747, 755, 819, 820
BseGI	GGATG	4 cut(s)	46, 739, 815, 1266
BseJI	GATNNNNATC	1 cut(s)	1209
BseLI	CCNNNNNNNGG	5 cut(s)	492, 684, 934, 935, 1187
BseMII	CTCAG	4 cut(s)	115, 248, 366, 1010
BseNI	ACTGG	5 cut(s)	86, 151, 193, 413, 1072
BseRI	GAGGAG	1 cut(s)	1203
BseSI	GKGCMC	1 cut(s)	941
BseXI	GCAGC	2 cut(s)	300, 508
BseYI	CCCAGC	1 cut(s)	48
Bsh1236I	CGCG	1 cut(s)	525
BshFI	GGCC	4 cut(s)	276, 412, 847, 1075
BshNI	GGYRCC	1 cut(s)	938
BshVI	ATCGAT	1 cut(s)	538
BsiHKAI	GWGCWC	1 cut(s)	1395
BsiHKCI	CYCGRG	1 cut(s)	63
BsiSI	CCGG	3 cut(s)	653, 1214, 1281
BslFI	GGGAC	1 cut(s)	911
BslI	CCNNNNNNNGG	5 cut(s)	492, 684, 934, 935, 1187
BsmFI	GGGAC	1 cut(s)	911
BsmI	GAATGC	1 cut(s)	246
BsnI	GGCC	4 cut(s)	276, 412, 847, 1075
BsoBI	CYCGRG	1 cut(s)	63
Bsp1286I	GDGCHC	2 cut(s)	941, 1395
Bsp13I	TCCGGA	1 cut(s)	652
Bsp143I	GATC	4 cut(s)	85, 535, 610, 1210
Bsp19I	CCATGG	1 cut(s)	213
Bsp68I	TCGCGA	1 cut(s)	525
BspACI	CCGC	3 cut(s)	1148, 1412, 1493
BspANI	GGCC	4 cut(s)	276, 412, 847, 1075
BspCNI	CTCAG	4 cut(s)	114, 247, 365, 1011
BspDI	ATCGAT	1 cut(s)	538
BspEI	TCCGGA	1 cut(s)	652
BspFNI	CGCG	1 cut(s)	525
BspLI	GGNNCC	2 cut(s)	753, 940
BspMAI	CTGCAG	1 cut(s)	349
BspMI	ACCTGC	1 cut(s)	856
BspOI	GCTAGC	2 cut(s)	1148, 1254
BspPI	GGATC	2 cut(s)	93, 1205
BspQI	GCTCTTC	2 cut(s)	522, 1134
BspT107I	GGYRCC	1 cut(s)	938
BsrI	ACTGG	5 cut(s)	86, 151, 193, 413, 1072
BssECI	CCNNGG	6 cut(s)	213, 491, 747, 755, 819, 820
BssMI	GATC	4 cut(s)	85, 535, 610, 1210
BssT1I	CCWWGG	4 cut(s)	213, 491, 747, 755
Bst2UI	CCWGG	2 cut(s)	821, 858
Bst4CI	ACNGT	2 cut(s)	125, 166
Bst6I	CTCTTC	3 cut(s)	522, 651, 1134
BstC8I	GCNNGC	5 cut(s)	1073, 1146, 1248, 1252, 1372
BstDEI	CTNAG	6 cut(s)	101, 234, 352, 614, 620, 1019
BstDSI	CCRYGG	1 cut(s)	213
BstENI	CCTNNNNNAGG	1 cut(s)	490
BstF5I	GGATG	4 cut(s)	46, 739, 815, 1266
BstFNI	CGCG	1 cut(s)	525
BstH2I	RGCGCY	1 cut(s)	1019
BstHHI	GCGC	1 cut(s)	1018
BstKTI	GATC	4 cut(s)	88, 538, 613, 1213
BstMBI	GATC	4 cut(s)	85, 535, 610, 1210
BstMWI	GCNNNNNNNGC	6 cut(s)	322, 853, 862, 1081, 1103, 1337
BstNI	CCWGG	2 cut(s)	821, 858
BstNSI	RCATGY	1 cut(s)	1374
BstSCI	CCNGG	3 cut(s)	819, 856, 1280
BstSFI	CTRYAG	2 cut(s)	345, 1041
BstSLI	GKGCMC	1 cut(s)	941
BstUI	CGCG	1 cut(s)	525
BstV1I	GCAGC	2 cut(s)	300, 508
BstV2I	GAAGAC	1 cut(s)	186
BstX2I	RGATCY	1 cut(s)	85
BstYI	RGATCY	1 cut(s)	85
Bsu15I	ATCGAT	1 cut(s)	538
BsuI	GTATCC	1 cut(s)	784
BsuRI	GGCC	4 cut(s)	276, 412, 847, 1075
BsuTUI	ATCGAT	1 cut(s)	538
BtgI	CCRYGG	1 cut(s)	213
BtsCI	GGATG	4 cut(s)	46, 739, 815, 1266
BtsI	GCAGTG	1 cut(s)	342
BtsIMutI	CAGTG	3 cut(s)	109, 130, 342
BtuMI	TCGCGA	1 cut(s)	525
BveI	ACCTGC	1 cut(s)	856
Cac8I	GCNNGC	5 cut(s)	1073, 1146, 1248, 1252, 1372
CfoI	GCGC	1 cut(s)	1018
Cfr13I	GGNCC	2 cut(s)	210, 1278
ClaI	ATCGAT	1 cut(s)	538
Csp6I	GTAC	3 cut(s)	144, 401, 1058
CviAII	CATG	2 cut(s)	214, 1371
CviQI	GTAC	3 cut(s)	144, 401, 1058
DdeI	CTNAG	6 cut(s)	101, 234, 352, 614, 620, 1019
DpnI	GATC	4 cut(s)	87, 537, 612, 1212
DpnII	GATC	4 cut(s)	85, 535, 610, 1210
EaeI	YGGCCR	2 cut(s)	410, 1073
Eam1104I	CTCTTC	3 cut(s)	522, 651, 1134
EarI	CTCTTC	3 cut(s)	522, 651, 1134
Ecl136II	GAGCTC	1 cut(s)	1393
Eco130I	CCWWGG	4 cut(s)	213, 491, 747, 755
Eco147I	AGGCCT	1 cut(s)	276
Eco24I	GRGCYC	1 cut(s)	1395
Eco47I	GGWCC	2 cut(s)	210, 1278
Eco47III	AGCGCT	1 cut(s)	1017
Eco53kI	GAGCTC	1 cut(s)	1393
Eco57I	CTGAAG	1 cut(s)	240
Eco88I	CYCGRG	1 cut(s)	63
EcoICRI	GAGCTC	1 cut(s)	1393
EcoNI	CCTNNNNNAGG	1 cut(s)	490
EcoRI	GAATTC	1 cut(s)	696
EcoRII	CCWGG	2 cut(s)	819, 856
EcoT14I	CCWWGG	4 cut(s)	213, 491, 747, 755
EcoT38I	GRGCYC	1 cut(s)	1395
ErhI	CCWWGG	4 cut(s)	213, 491, 747, 755
FaeI	CATG	2 cut(s)	217, 1374
FalI	AAGNNNNNCTT	4 cut(s)	484, 516, 731, 763
FaqI	GGGAC	1 cut(s)	911
FatI	CATG	2 cut(s)	213, 1370
FauI	CCCGC	1 cut(s)	1500
FauNDI	CATATG	1 cut(s)	916
FblI	GTMKAC	1 cut(s)	1160
Fnu4HI	GCNGC	2 cut(s)	314, 497
FokI	GGATG	4 cut(s)	53, 746, 802, 1273
FriOI	GRGCYC	1 cut(s)	1395
Fsp4HI	GCNGC	2 cut(s)	314, 497
FspBI	CTAG	8 cut(s)	110, 180, 419, 948, 1145, 1251, 1487, 1496
GlaI	GCGC	1 cut(s)	1017
GluI	GCNGC	2 cut(s)	314, 497
GsaI	CCCAGC	1 cut(s)	52
HaeII	RGCGCY	1 cut(s)	1019
HaeIII	GGCC	4 cut(s)	276, 412, 847, 1075
HapII	CCGG	3 cut(s)	653, 1214, 1281
HhaI	GCGC	1 cut(s)	1018
Hin1II	CATG	2 cut(s)	217, 1374
Hin6I	GCGC	1 cut(s)	1016
HinP1I	GCGC	1 cut(s)	1016
HincII	GTYRAC	1 cut(s)	121
HindII	GTYRAC	1 cut(s)	121
HindIII	AAGCTT	2 cut(s)	1173, 1230
HinfI	GANTC	6 cut(s)	176, 350, 520, 772, 953, 1292
HpaII	CCGG	3 cut(s)	653, 1214, 1281
HphI	GGTGA	1 cut(s)	872
Hpy166II	GTNNAC	4 cut(s)	107, 121, 1153, 1161
Hpy188I	TCNGA	6 cut(s)	237, 355, 432, 988, 1081, 1182
Hpy188III	TCNNGA	5 cut(s)	63, 180, 462, 524, 653
Hpy8I	GTNNAC	4 cut(s)	107, 121, 1153, 1161
HpyAV	CCTTC	4 cut(s)	168, 688, 733, 1376
HpyCH4III	ACNGT	2 cut(s)	125, 166
HpyCH4IV	ACGT	1 cut(s)	794
HpyCH4V	TGCA	2 cut(s)	347, 1246
HpyF10VI	GCNNNNNNNGC	6 cut(s)	322, 853, 862, 1081, 1103, 1337
HpyF3I	CTNAG	6 cut(s)	101, 234, 352, 614, 620, 1019
HpySE526I	ACGT	1 cut(s)	794
Hsp92II	CATG	2 cut(s)	217, 1374
HspAI	GCGC	1 cut(s)	1016
Kpn2I	TCCGGA	1 cut(s)	652
Kzo9I	GATC	4 cut(s)	85, 535, 610, 1210
LguI	GCTCTTC	2 cut(s)	522, 1134
LmnI	GCTCC	1 cut(s)	1081
Lsp1109I	GCAGC	2 cut(s)	300, 508
LweI	GCATC	2 cut(s)	77, 560
MaeI	CTAG	8 cut(s)	110, 180, 419, 948, 1145, 1251, 1487, 1496
MaeII	ACGT	1 cut(s)	794
MaeIII	GTNAC	2 cut(s)	446, 583
MalI	GATC	4 cut(s)	87, 537, 612, 1212
MboI	GATC	4 cut(s)	85, 535, 610, 1210
MboII	GAAGA	8 cut(s)	186, 301, 450, 539, 668, 898, 1151, 1180
MfeI	CAATTG	2 cut(s)	573, 1421
MflI	RGATCY	1 cut(s)	85
MhlI	GDGCHC	2 cut(s)	941, 1395
MlsI	TGGCCA	2 cut(s)	412, 1075
MluCI	AATT	9 cut(s)	169, 394, 573, 696, 1062, 1133, 1221, 1306, 1421
MluNI	TGGCCA	2 cut(s)	412, 1075
MlyI	GAGTC	2 cut(s)	344, 514
MmeI	TCCRAC	2 cut(s)	577, 990
Mox20I	TGGCCA	2 cut(s)	412, 1075
MroI	TCCGGA	1 cut(s)	652
MscI	TGGCCA	2 cut(s)	412, 1075
MseI	TTAA	3 cut(s)	24, 168, 1268
MslI	CAYNNNNRTG	3 cut(s)	39, 780, 1259
Msp20I	TGGCCA	2 cut(s)	412, 1075
MspI	CCGG	3 cut(s)	653, 1214, 1281
MspR9I	CCNGG	3 cut(s)	821, 858, 1282
MunI	CAATTG	2 cut(s)	573, 1421
Mva1269I	GAATGC	1 cut(s)	246
MvaI	CCWGG	2 cut(s)	821, 858
MvnI	CGCG	1 cut(s)	525
MwoI	GCNNNNNNNGC	6 cut(s)	322, 853, 862, 1081, 1103, 1337
NciI	CCSGG	1 cut(s)	1282
NcoI	CCATGG	1 cut(s)	213
NdeI	CATATG	1 cut(s)	916
NdeII	GATC	4 cut(s)	85, 535, 610, 1210
NheI	GCTAGC	2 cut(s)	1144, 1250
NlaIII	CATG	2 cut(s)	217, 1374
NlaIV	GGNNCC	2 cut(s)	753, 940
NruI	TCGCGA	1 cut(s)	525
NspI	RCATGY	1 cut(s)	1374
PaeI	GCATGC	1 cut(s)	1374
PaeR7I	CTCGAG	1 cut(s)	63
PasI	CCCWGGG	1 cut(s)	820
PceI	AGGCCT	1 cut(s)	276
PciSI	GCTCTTC	2 cut(s)	522, 1134
PcsI	WCGNNNNNNNCGW	1 cut(s)	715
PctI	GAATGC	1 cut(s)	246
PfeI	GAWTC	4 cut(s)	176, 772, 953, 1292
PkrI	GCNGC	2 cut(s)	315, 498
PleI	GAGTC	2 cut(s)	344, 514
PpsI	GAGTC	2 cut(s)	344, 514
PsiI	TTATAA	1 cut(s)	999
Psp124BI	GAGCTC	1 cut(s)	1395
Psp6I	CCWGG	2 cut(s)	819, 856
PspFI	CCCAGC	1 cut(s)	48
PspGI	CCWGG	2 cut(s)	819, 856
PspN4I	GGNNCC	2 cut(s)	753, 940
PspPI	GGNCC	2 cut(s)	210, 1278
PstI	CTGCAG	1 cut(s)	349
PsuI	RGATCY	1 cut(s)	85
RruI	TCGCGA	1 cut(s)	525
RsaI	GTAC	3 cut(s)	145, 402, 1059
RsaNI	GTAC	3 cut(s)	144, 401, 1058
RseI	CAYNNNNRTG	3 cut(s)	39, 780, 1259
SacI	GAGCTC	1 cut(s)	1395
SapI	GCTCTTC	2 cut(s)	522, 1134
SaqAI	TTAA	3 cut(s)	24, 168, 1268
SatI	GCNGC	2 cut(s)	314, 497
Sau3AI	GATC	4 cut(s)	85, 535, 610, 1210
Sau96I	GGNCC	2 cut(s)	210, 1278
ScaI	AGTACT	1 cut(s)	145
SchI	GAGTC	2 cut(s)	344, 514
ScrFI	CCNGG	3 cut(s)	821, 858, 1282
SduI	GDGCHC	2 cut(s)	941, 1395
SfaNI	GCATC	2 cut(s)	77, 560
SfcI	CTRYAG	2 cut(s)	345, 1041
Sfr274I	CTCGAG	1 cut(s)	63
SinI	GGWCC	2 cut(s)	210, 1278
SlaI	CTCGAG	1 cut(s)	63
SmiMI	CAYNNNNRTG	3 cut(s)	39, 780, 1259
SmlI	CTYRAG	1 cut(s)	63
SmoI	CTYRAG	1 cut(s)	63
SphI	GCATGC	1 cut(s)	1374
Sse9I	AATT	9 cut(s)	169, 394, 573, 696, 1062, 1133, 1221, 1306, 1421
SseBI	AGGCCT	1 cut(s)	276
SsiI	CCGC	3 cut(s)	1148, 1412, 1493
SspI	AATATT	1 cut(s)	154
SspMI	CTAG	8 cut(s)	110, 180, 419, 948, 1145, 1251, 1487, 1496
SstI	GAGCTC	1 cut(s)	1395
StuI	AGGCCT	1 cut(s)	276
StyD4I	CCNGG	3 cut(s)	819, 856, 1280
StyI	CCWWGG	4 cut(s)	213, 491, 747, 755
TaaI	ACNGT	2 cut(s)	125, 166
TaiI	ACGT	1 cut(s)	797
TaqI	TCGA	6 cut(s)	64, 461, 538, 718, 1295, 1352
TasI	AATT	9 cut(s)	169, 394, 573, 696, 1062, 1133, 1221, 1306, 1421
TatI	WGTACW	1 cut(s)	143
TfiI	GAWTC	4 cut(s)	176, 772, 953, 1292
Tru1I	TTAA	3 cut(s)	24, 168, 1268
Tru9I	TTAA	3 cut(s)	24, 168, 1268
TscAI	CASTG	3 cut(s)	109, 130, 349
TseI	GCWGC	2 cut(s)	313, 496
TspDTI	ATGAA	5 cut(s)	328, 1139, 1181, 1251, 1349
TspGWI	ACGGA	1 cut(s)	946
TspRI	CASTG	3 cut(s)	109, 130, 349
VpaK11BI	GGWCC	2 cut(s)	210, 1278
XagI	CCTNNNNNAGG	1 cut(s)	490
XapI	RAATTY	2 cut(s)	394, 696
XbaI	TCTAGA	1 cut(s)	179
XceI	RCATGY	1 cut(s)	1374
XhoI	CTCGAG	1 cut(s)	63
XmiI	GTMKAC	1 cut(s)	1160
XspI	CTAG	8 cut(s)	110, 180, 419, 948, 1145, 1251, 1487, 1496
ZrmI	AGTACT	1 cut(s)	145

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.