Rorug02G0449800

Ribulose-1,5 bisphosphate carboxylase oxygenase large subunit N-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000405
Physical Location & Seq
Reverse (-)
57719371 .. 57720996
1626 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0449800.1

Sequence Viewer

Length: 324 bp
ATGCTTGTGGATGATGGAGGGGAAGTTGTGACTGCTTCGGAGTGCCCCAACTGCCGGAGACTCTTTTGTGCTCAATGCAGGGTTGTGTGGCATGCAGGGATTGATTGTAGTGAGTTTCAGAGGTTGAGTAGGGATGAAAGAGGGATGGAAGATATCATGGTGATGGAACTTGCTAAGAAACAGCAATGGAGGAGATGCCCAAGGTGCAAGTTCTATGTGGAGAAGACTGCTGGCTGCTCACACATTACTTGCAGGTGTGGATTCGAATTTTTCTATGGCTGTGGATCCAATTGGAGTAATTGCCATGGATGTAGAGCGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00310 map00310
Pfam Domains
Protein Families
Rubis-subs-bind SET

Protein Analysis

107

Amino Acids

12.32

Weight (kDa)

7.96

Isoelectric Point (pI)

46.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IBR PF01485 13 - 36 3.1e-06 IBR domain, a half RING-finger domain
IBR PF01485 57 - 101 3.5e-06 IBR domain, a half RING-finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000405)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25330
fragaria_vesca FvH4_2g12920 FvH4_2g12920 FvH4_2g12920 FvH4_2g12920 FvH4_3g17720 FvH4_3g17720 FvH4_3g20410 FvH4_3g20410 FvH4_5g23730 FvH4_5g23730 FvH4_5g23730 FvH4_6g40510 FvH4_6g40520 FvH4_6g40530 FvH4_6g40530
malus_domestica MD09G1128800.v1.1 MD09G1128900.v1.1 MD09G1129100.v1.1 MD17G1118100.v1.1
prunus_persica Prupe.3G196400_v2.0.a1 Prupe.3G196400_v2.0.a1 Prupe.3G196400_v2.0.a1 Prupe.3G196400_v2.0.a1 Prupe.3G196400_v2.0.a1 Prupe.3G196500_v2.0.a1 Prupe.3G196500_v2.0.a1
pyrus_communis pycom09g05230 pycom09g05240 pycom10g00180 pycom17g10860
rosa_chinensis RchiOBHm_Chr2g0133241 RchiOBHm_Chr2g0133311 RchiOBHm_Chr2g0155531 RchiOBHm_Chr2g0155771 RchiOBHm_Chr2g0155781 RchiOBHm_Chr2g0155791 RchiOBHm_Chr5g0029431 RchiOBHm_Chr5g0034301 RchiOBHm_Chr7g0213061
rosa_laevigata RLG00000002835 RLG00000019323 RLG00000020863 RLG00000020864 RLG00000033165 RLG00000033538
rosa_multiflora Rmu_co8060268.1_g000001 Rmu_sc0000062.1_g000021 Rmu_sc0001121.1_g000020 Rmu_sc0001446.1_g000013 Rmu_sc0002712.1_g000021 Rmu_sc0003417.1_g000005 Rmu_sc0023254.1_g000004 Rmu_sc0024719.1_g000001 Rmu_sc0033434.1_g000002
rosa_roxburghii Rroxscaffold_1G00045970 Rroxscaffold_2G00093480 Rroxscaffold_2G00093490 Rroxscaffold_2G00093500 Rroxscaffold_2G00111280 Rroxscaffold_3G00246280 Rroxscaffold_7G00195910
rosa_rugosa Rorug02G0310900 Rorug02G0449800 Rorug02G0449900 Rorug04G0061300 Rorug05G0145100 Rorug06G0068700 Rorug07G0139100
rosa_samantha Rh2AG363400 Rh2AG515900 Rh2AG516000 Rh2BG369100 Rh2BG525000 Rh2BG526900 Rh2BG527000 Rh2CG346700 Rh2CG500900 Rh2CG501000 Rh2DG386100 Rh2DG535900 Rh2DG536000 Rh5AG236700 Rh5BG204800 Rh5BG237200 Rh5CG226800 Rh5CG267000 Rh5DG208400 Rh5DG244700 Rh7AG270400 Rh7AG270500 Rh7BG266000 Rh7CG289700 Rh7CG289800 Rh7DG278700
rosa_wichuraiana Rw2G029570 Rw2G042430 Rw2G042440 Rw5G021290 Rw7G023500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 243
Acc36I ACCTGC 1 cut(s) 243
AclWI GGATC 2 cut(s) 279, 292
AcsI RAATTY 1 cut(s) 266
AfiI CCNNNNNNNGG 1 cut(s) 54
Alw21I GWGCWC 1 cut(s) 73
Alw26I GTCTC 1 cut(s) 52
AlwI GGATC 2 cut(s) 279, 292
ApeKI GCWGC 1 cut(s) 234
ApoI RAATTY 1 cut(s) 266
AsuHPI GGTGA 1 cut(s) 172
AsuII TTCGAA 1 cut(s) 264
BaeGI GKGCMC 1 cut(s) 47
BamHI GGATCC 1 cut(s) 284
BbsI GAAGAC 1 cut(s) 230
Bbv12I GWGCWC 1 cut(s) 73
BbvI GCAGC 1 cut(s) 221
BccI CCATC 3 cut(s) 8, 139, 157
BcoDI GTCTC 1 cut(s) 52
BfuAI ACCTGC 1 cut(s) 243
BisI GCNGC 1 cut(s) 235
BlsI GCNGC 1 cut(s) 236
BmiI GGNNCC 1 cut(s) 286
BmsI GCATC 1 cut(s) 185
BpiI GAAGAC 1 cut(s) 230
Bpu14I TTCGAA 1 cut(s) 264
BsaJI CCNNGG 2 cut(s) 200, 304
BsaXI ACNNNNNCTCC 2 cut(s) 9, 39
Bsc4I CCNNNNNNNGG 1 cut(s) 54
Bse3DI GCAATG 1 cut(s) 191
BseDI CCNNGG 2 cut(s) 200, 304
BseGI GGATG 4 cut(s) 16, 139, 150, 314
BseLI CCNNNNNNNGG 1 cut(s) 54
BseMI GCAATG 1 cut(s) 191
BseRI GAGGAG 1 cut(s) 205
BseSI GKGCMC 1 cut(s) 47
BseXI GCAGC 1 cut(s) 221
BsiHKAI GWGCWC 1 cut(s) 73
BsiSI CCGG 1 cut(s) 55
BslI CCNNNNNNNGG 1 cut(s) 54
BsmAI GTCTC 1 cut(s) 52
Bsp119I TTCGAA 1 cut(s) 264
Bsp1286I GDGCHC 2 cut(s) 47, 73
Bsp143I GATC 1 cut(s) 284
Bsp19I CCATGG 1 cut(s) 304
BspLI GGNNCC 1 cut(s) 286
BspMI ACCTGC 1 cut(s) 243
BspPI GGATC 2 cut(s) 279, 292
BspT104I TTCGAA 1 cut(s) 264
BsrDI GCAATG 1 cut(s) 191
BssECI CCNNGG 2 cut(s) 200, 304
BssMI GATC 1 cut(s) 284
BssT1I CCWWGG 2 cut(s) 200, 304
BstBI TTCGAA 1 cut(s) 264
BstC8I GCNNGC 2 cut(s) 93, 232
BstDEI CTNAG 1 cut(s) 174
BstDSI CCRYGG 1 cut(s) 304
BstF5I GGATG 4 cut(s) 16, 139, 150, 314
BstKTI GATC 1 cut(s) 287
BstMAI GTCTC 1 cut(s) 52
BstMBI GATC 1 cut(s) 284
BstMWI GCNNNNNNNGC 2 cut(s) 51, 204
BstNSI RCATGY 1 cut(s) 95
BstSLI GKGCMC 1 cut(s) 47
BstV1I GCAGC 1 cut(s) 221
BstV2I GAAGAC 1 cut(s) 230
BstX2I RGATCY 1 cut(s) 284
BstYI RGATCY 1 cut(s) 284
BtgI CCRYGG 1 cut(s) 304
BtsCI GGATG 4 cut(s) 16, 139, 150, 314
BveI ACCTGC 1 cut(s) 243
Cac8I GCNNGC 2 cut(s) 93, 232
CviAII CATG 3 cut(s) 92, 157, 305
CviJI RGCY 2 cut(s) 234, 279
CviKI_1 RGCY 2 cut(s) 234, 279
DdeI CTNAG 1 cut(s) 174
DpnI GATC 1 cut(s) 286
DpnII GATC 1 cut(s) 284
Eco130I CCWWGG 2 cut(s) 200, 304
Eco32I GATATC 1 cut(s) 154
EcoRV GATATC 1 cut(s) 154
EcoT14I CCWWGG 2 cut(s) 200, 304
ErhI CCWWGG 2 cut(s) 200, 304
FaeI CATG 3 cut(s) 95, 160, 308
FaiI YATR 5 cut(s) 93, 158, 216, 276, 306
FatI CATG 3 cut(s) 91, 156, 304
Fnu4HI GCNGC 1 cut(s) 235
FokI GGATG 3 cut(s) 23, 146, 157
Fsp4HI GCNGC 1 cut(s) 235
GluI GCNGC 1 cut(s) 235
HapII CCGG 1 cut(s) 55
Hin1II CATG 3 cut(s) 95, 160, 308
HinfI GANTC 2 cut(s) 60, 261
HpaII CCGG 1 cut(s) 55
HphI GGTGA 1 cut(s) 172
Hpy188I TCNGA 2 cut(s) 40, 120
HpyCH4V TGCA 4 cut(s) 78, 95, 207, 252
HpyF10VI GCNNNNNNNGC 2 cut(s) 51, 204
HpyF3I CTNAG 1 cut(s) 174
Hsp92II CATG 3 cut(s) 95, 160, 308
Kzo9I GATC 1 cut(s) 284
LpnPI CCDG 5 cut(s) 64, 68, 81, 216, 238
Lsp1109I GCAGC 1 cut(s) 221
LweI GCATC 1 cut(s) 185
MaeIII GTNAC 1 cut(s) 28
MalI GATC 1 cut(s) 286
MboI GATC 1 cut(s) 284
MboII GAAGA 2 cut(s) 161, 235
MfeI CAATTG 1 cut(s) 289
MflI RGATCY 1 cut(s) 284
MhlI GDGCHC 2 cut(s) 47, 73
MluCI AATT 3 cut(s) 266, 289, 298
MlyI GAGTC 1 cut(s) 54
MnlI CCTC 4 cut(s) 11, 114, 134, 183
MslI CAYNNNNRTG 1 cut(s) 161
MspI CCGG 1 cut(s) 55
MunI CAATTG 1 cut(s) 289
MwoI GCNNNNNNNGC 2 cut(s) 51, 204
NcoI CCATGG 1 cut(s) 304
NdeII GATC 1 cut(s) 284
NlaIII CATG 3 cut(s) 95, 160, 308
NlaIV GGNNCC 1 cut(s) 286
NmuCI GTSAC 1 cut(s) 28
NspI RCATGY 1 cut(s) 95
NspV TTCGAA 1 cut(s) 264
PaeI GCATGC 1 cut(s) 95
PaqCI CACCTGC 1 cut(s) 243
PfeI GAWTC 1 cut(s) 261
PkrI GCNGC 1 cut(s) 236
PleI GAGTC 1 cut(s) 54
PpsI GAGTC 1 cut(s) 54
PspN4I GGNNCC 1 cut(s) 286
PsuI RGATCY 1 cut(s) 284
RseI CAYNNNNRTG 1 cut(s) 161
SatI GCNGC 1 cut(s) 235
Sau3AI GATC 1 cut(s) 284
SchI GAGTC 1 cut(s) 54
SduI GDGCHC 2 cut(s) 47, 73
SetI ASST 3 cut(s) 125, 206, 257
SfaNI GCATC 1 cut(s) 185
SfuI TTCGAA 1 cut(s) 264
SmiMI CAYNNNNRTG 1 cut(s) 161
SphI GCATGC 1 cut(s) 95
Sse9I AATT 3 cut(s) 266, 289, 298
StyI CCWWGG 2 cut(s) 200, 304
TaqI TCGA 1 cut(s) 264
TasI AATT 3 cut(s) 266, 289, 298
TfiI GAWTC 1 cut(s) 261
TseFI GTSAC 1 cut(s) 28
TseI GCWGC 1 cut(s) 234
Tsp45I GTSAC 1 cut(s) 28
TspDTI ATGAA 1 cut(s) 150
XapI RAATTY 1 cut(s) 266
XceI RCATGY 1 cut(s) 95
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.