Rh7BG266000

Isoflavone reductase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0000405
Physical Location & Seq
Reverse (-)
24357481 .. 24362339
4859 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG266000.1

Sequence Viewer

Length: 1191 bp
ATGGATTTGGAGTTCAGGTCGTGCGTGGACGACGCGTGGTACCACGTCCGAATAGAGACGGACGGTGACGGCGGGGATCGACTGAGAGTCAAGTTCGTCGGCTTCTCCAACGATCACGACGAGGTTTATGACACCGAGGAGTTGACGTCGTTTAGAGACGTGGAGGAGTTTAGGTCGAGGTTCAGGCGTATGTCGATTCAGGTTCAAGACTGGGAGTGCAGAAAGGTGGTGAAGGGGACGGTCGTGTGTGCAGCCATTTCAATTGGCCCCGACGACTGCCGCTTCTACGACGCCGTTGTTGATCGGGTGGTACATAAGGAACATCGGCTCGTAAAAGGAAAACAAGAATGCTCATGCAACTTCATTCTATTTTGGAGACATGGTCCAAAAGCTCGATCATTAACTGCTCAAACTCTTGAAAACATTTGCAGAGTGCAACCTCCTGTGGACAAATTAGATCCGCTGCTGACCACTTTTCTTGACACAGCAACGGAAAAAATAGAAACCATTCTTTCCAGTATTTTCAAAGTTCCTCATGAAGCTTCTCATCACAGAGAACCTTGTACACCCAACTGCAGGGAAGAGCTAAGCTTTTCTAAACGTCTAAACAAGGATACAAGCCTTTCCAGGCGATCTTTAAGTAAAGAAATGATTGACAATCCTTTGGAAAAGGATGAACATGACATGGACATAGGAGGTGTTCCTTATATGTTACTGGTTGAGAACCTTGAGAAGGGGATATCACCATTCACAATTATGGAATTCATACACCAGCAAGCTACAATCTCATGTCAAGCATCTGTTTGGCCAAGTAAGTCATCAGAGGCATATACAAGAGGAACCATCATAGTTGACAGCAAAAGAAATCTGGAAAAGATATCTGACTTTTTAGAGAATCCAGATCACGTTATCATTTCCTCAAAAGGAAGGCCGTGGGTAATGACTGAGAAATCCGCATTACATGAGACACCAGGGGCATCGATACAAAGCTTCATGCTCATGTCTCAGACAATATTACAGAATAGAAGCCCTACAACGAACAATGGATTGAAGGTTGTCATTTCTGGAACTAAAGAGTATGAGACAGCTAAGTTGCTGAAGGATTTGTTTTTGGCATTTGTAAAGCATCAAAGTCGACTTCACAGGAGGTTACTTATAGTAGAGGGAAAGATCTCGCATTTGATGCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NmrA

Protein Analysis

396

Amino Acids

45.43

Weight (kDa)

6.44

Isoelectric Point (pI)

47.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAWADEE PF16719 1 - 143 3.8e-32 SAWADEE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000405)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25330
fragaria_vesca FvH4_2g12920 FvH4_2g12920 FvH4_2g12920 FvH4_2g12920 FvH4_3g17720 FvH4_3g17720 FvH4_3g20410 FvH4_3g20410 FvH4_5g23730 FvH4_5g23730 FvH4_5g23730 FvH4_6g40510 FvH4_6g40520 FvH4_6g40530 FvH4_6g40530
malus_domestica MD09G1128800.v1.1 MD09G1128900.v1.1 MD09G1129100.v1.1 MD17G1118100.v1.1
prunus_persica Prupe.3G196400_v2.0.a1 Prupe.3G196400_v2.0.a1 Prupe.3G196400_v2.0.a1 Prupe.3G196400_v2.0.a1 Prupe.3G196400_v2.0.a1 Prupe.3G196500_v2.0.a1 Prupe.3G196500_v2.0.a1
pyrus_communis pycom09g05230 pycom09g05240 pycom10g00180 pycom17g10860
rosa_chinensis RchiOBHm_Chr2g0133241 RchiOBHm_Chr2g0133311 RchiOBHm_Chr2g0155531 RchiOBHm_Chr2g0155771 RchiOBHm_Chr2g0155781 RchiOBHm_Chr2g0155791 RchiOBHm_Chr5g0029431 RchiOBHm_Chr5g0034301 RchiOBHm_Chr7g0213061
rosa_laevigata RLG00000002835 RLG00000019323 RLG00000020863 RLG00000020864 RLG00000033165 RLG00000033538
rosa_multiflora Rmu_co8060268.1_g000001 Rmu_sc0000062.1_g000021 Rmu_sc0001121.1_g000020 Rmu_sc0001446.1_g000013 Rmu_sc0002712.1_g000021 Rmu_sc0003417.1_g000005 Rmu_sc0023254.1_g000004 Rmu_sc0024719.1_g000001 Rmu_sc0033434.1_g000002
rosa_roxburghii Rroxscaffold_1G00045970 Rroxscaffold_2G00093480 Rroxscaffold_2G00093490 Rroxscaffold_2G00093500 Rroxscaffold_2G00111280 Rroxscaffold_3G00246280 Rroxscaffold_7G00195910
rosa_rugosa Rorug02G0310900 Rorug02G0449800 Rorug02G0449900 Rorug04G0061300 Rorug05G0145100 Rorug06G0068700 Rorug07G0139100
rosa_samantha Rh2AG363400 Rh2AG515900 Rh2AG516000 Rh2BG369100 Rh2BG525000 Rh2BG526900 Rh2BG527000 Rh2CG346700 Rh2CG500900 Rh2CG501000 Rh2DG386100 Rh2DG535900 Rh2DG536000 Rh5AG236700 Rh5BG204800 Rh5BG237200 Rh5CG226800 Rh5CG267000 Rh5DG208400 Rh5DG244700 Rh7AG270400 Rh7AG270500 Rh7BG266000 Rh7CG289700 Rh7CG289800 Rh7DG278700
rosa_wichuraiana Rw2G029570 Rw2G042430 Rw2G042440 Rw5G021290 Rw7G023500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 149
Acc65I GGTACC 1 cut(s) 39
AccB1I GGYRCC 1 cut(s) 39
AccI GTMKAC 1 cut(s) 1135
AccII CGCG 1 cut(s) 35
AciI CCGC 4 cut(s) 72, 280, 461, 954
AclWI GGATC 2 cut(s) 84, 452
AcoI YGGCCR 1 cut(s) 806
AcsI RAATTY 1 cut(s) 761
AcuI CTGAAG 1 cut(s) 1118
AcyI GRCGYC 2 cut(s) 146, 291
AfaI GTAC 3 cut(s) 41, 312, 565
AfiI CCNNNNNNNGG 2 cut(s) 576, 733
AflIII ACRYGT 1 cut(s) 33
AgsI TTSAA 5 cut(s) 206, 261, 419, 526, 1051
AhdI GACNNNNNGTC 1 cut(s) 86
AjiI CACGTC 2 cut(s) 46, 160
AjnI CCWGG 2 cut(s) 626, 970
AloI GAACNNNNNNTCC 1 cut(s) 28
AluBI AGCT 7 cut(s) 392, 542, 586, 591, 779, 990, 1088
AluI AGCT 7 cut(s) 392, 542, 586, 591, 779, 990, 1088
Alw26I GTCTC 6 cut(s) 50, 150, 370, 959, 1008, 1076
AlwI GGATC 2 cut(s) 84, 452
AoxI GGCC 3 cut(s) 265, 806, 929
ApeKI GCWGC 2 cut(s) 251, 463
ApoI RAATTY 1 cut(s) 761
Asp700I GAANNNNTTC 1 cut(s) 507
Asp718I GGTACC 1 cut(s) 39
AspS9I GGNCC 2 cut(s) 266, 383
AsuHPI GGTGA 3 cut(s) 77, 241, 735
AvaII GGWCC 1 cut(s) 383
BalI TGGCCA 1 cut(s) 808
BanI GGYRCC 1 cut(s) 39
BbvI GCAGC 2 cut(s) 263, 450
BccI CCATC 1 cut(s) 851
BceAI ACGGC 3 cut(s) 85, 278, 916
BcgI CGANNNNNNTGC 2 cut(s) 1115, 1149
BciT130I CCWGG 2 cut(s) 628, 972
BciVI GTATCC 1 cut(s) 607
BcoDI GTCTC 6 cut(s) 50, 150, 370, 959, 1008, 1076
BfmI CTRYAG 1 cut(s) 574
BfuI GTATCC 1 cut(s) 607
BglII AGATCT 1 cut(s) 1170
BisI GCNGC 3 cut(s) 252, 280, 464
BlpI GCTNAGC 1 cut(s) 587
BlsI GCNGC 3 cut(s) 253, 281, 465
Bme1390I CCNGG 2 cut(s) 628, 972
Bme18I GGWCC 1 cut(s) 383
BmeRI GACNNNNNGTC 1 cut(s) 86
BmgBI CACGTC 2 cut(s) 46, 160
BmgT120I GGNCC 2 cut(s) 266, 383
BmiI GGNNCC 3 cut(s) 41, 268, 841
BmrFI CCNGG 2 cut(s) 628, 972
BmrI ACTGGG 1 cut(s) 220
BmsI GCATC 4 cut(s) 806, 986, 1135, 1173
BmuI ACTGGG 1 cut(s) 220
Bpu1102I GCTNAGC 1 cut(s) 587
BpuEI CTTGAG 1 cut(s) 749
Bsa29I ATCGAT 1 cut(s) 980
BsaHI GRCGYC 2 cut(s) 146, 291
BsaJI CCNNGG 3 cut(s) 135, 932, 971
BsaXI ACNNNNNCTCC 7 cut(s) 32, 131, 158, 161, 188, 367, 397
Bsc4I CCNNNNNNNGG 2 cut(s) 576, 733
Bse1I ACTGG 3 cut(s) 215, 516, 720
BseBI CCWGG 2 cut(s) 628, 972
BseCI ATCGAT 1 cut(s) 980
BseDI CCNNGG 3 cut(s) 135, 932, 971
BseGI GGATG 1 cut(s) 679
BseLI CCNNNNNNNGG 2 cut(s) 576, 733
BseMII CTCAG 3 cut(s) 74, 936, 1019
BseNI ACTGG 3 cut(s) 215, 516, 720
BseRI GAGGAG 2 cut(s) 152, 179
BseXI GCAGC 2 cut(s) 263, 450
BsgI GTGCAG 2 cut(s) 238, 270
Bsh1236I CGCG 1 cut(s) 35
Bsh1285I CGRYCG 1 cut(s) 243
BshFI GGCC 3 cut(s) 267, 808, 931
BshNI GGYRCC 1 cut(s) 39
BshVI ATCGAT 1 cut(s) 980
BsiEI CGRYCG 1 cut(s) 243
BslFI GGGAC 1 cut(s) 250
BslI CCNNNNNNNGG 2 cut(s) 576, 733
BsmAI GTCTC 6 cut(s) 50, 150, 370, 959, 1008, 1076
BsmBI CGTCTC 2 cut(s) 50, 150
BsmFI GGGAC 1 cut(s) 250
BsmI GAATGC 1 cut(s) 353
BsnI GGCC 3 cut(s) 267, 808, 931
Bsp1407I TGTACA 1 cut(s) 563
Bsp143I GATC 8 cut(s) 76, 112, 301, 395, 457, 632, 901, 1170
Bsp1720I GCTNAGC 1 cut(s) 587
BspACI CCGC 4 cut(s) 72, 280, 461, 954
BspANI GGCC 3 cut(s) 267, 808, 931
BspCNI CTCAG 3 cut(s) 75, 937, 1018
BspDI ATCGAT 1 cut(s) 980
BspFNI CGCG 1 cut(s) 35
BspHI TCATGA 1 cut(s) 535
BspLI GGNNCC 3 cut(s) 41, 268, 841
BspMAI CTGCAG 1 cut(s) 578
BspPI GGATC 2 cut(s) 84, 452
BspQI GCTCTTC 1 cut(s) 576
BspT107I GGYRCC 1 cut(s) 39
BsrGI TGTACA 1 cut(s) 563
BsrI ACTGG 3 cut(s) 215, 516, 720
BssECI CCNNGG 3 cut(s) 135, 932, 971
BssMI GATC 8 cut(s) 76, 112, 301, 395, 457, 632, 901, 1170
BssNI GRCGYC 2 cut(s) 146, 291
Bst2UI CCWGG 2 cut(s) 628, 972
Bst4CI ACNGT 2 cut(s) 65, 241
Bst6I CTCTTC 1 cut(s) 576
BstACI GRCGYC 2 cut(s) 146, 291
BstAPI GCANNNNNTGC 1 cut(s) 1183
BstAUI TGTACA 1 cut(s) 563
BstC8I GCNNGC 1 cut(s) 777
BstDEI CTNAG 5 cut(s) 83, 587, 945, 1005, 1089
BstDSI CCRYGG 1 cut(s) 932
BstENI CCTNNNNNAGG 1 cut(s) 731
BstF5I GGATG 1 cut(s) 679
BstFNI CGCG 1 cut(s) 35
BstKTI GATC 8 cut(s) 79, 115, 304, 398, 460, 635, 904, 1173
BstMAI GTCTC 6 cut(s) 50, 150, 370, 959, 1008, 1076
BstMBI GATC 8 cut(s) 76, 112, 301, 395, 457, 632, 901, 1170
BstMCI CGRYCG 1 cut(s) 243
BstMWI GCNNNNNNNGC 1 cut(s) 1183
BstNI CCWGG 2 cut(s) 628, 972
BstSCI CCNGG 2 cut(s) 626, 970
BstSFI CTRYAG 1 cut(s) 574
BstUI CGCG 1 cut(s) 35
BstV1I GCAGC 2 cut(s) 263, 450
BstX2I RGATCY 2 cut(s) 457, 1170
BstYI RGATCY 2 cut(s) 457, 1170
Bsu15I ATCGAT 1 cut(s) 980
BsuI GTATCC 1 cut(s) 607
BsuRI GGCC 3 cut(s) 267, 808, 931
BsuTUI ATCGAT 1 cut(s) 980
BtgI CCRYGG 1 cut(s) 932
BtrI CACGTC 2 cut(s) 46, 160
BtsCI GGATG 1 cut(s) 679
Cac8I GCNNGC 1 cut(s) 777
CciI TCATGA 1 cut(s) 535
Cfr13I GGNCC 2 cut(s) 266, 383
ClaI ATCGAT 1 cut(s) 980
CseI GACGC 2 cut(s) 41, 299
Csp6I GTAC 3 cut(s) 40, 311, 564
CspCI CAANNNNNGTGG 2 cut(s) 460, 495
CviAII CATG 9 cut(s) 354, 380, 536, 680, 685, 789, 962, 994, 1000
CviQI GTAC 3 cut(s) 40, 311, 564
DdeI CTNAG 5 cut(s) 83, 587, 945, 1005, 1089
DpnI GATC 8 cut(s) 78, 114, 303, 397, 459, 634, 903, 1172
DpnII GATC 8 cut(s) 76, 112, 301, 395, 457, 632, 901, 1170
DriI GACNNNNNGTC 1 cut(s) 86
EaeI YGGCCR 1 cut(s) 806
Eam1104I CTCTTC 1 cut(s) 576
Eam1105I GACNNNNNGTC 1 cut(s) 86
EarI CTCTTC 1 cut(s) 576
Eco32I GATATC 2 cut(s) 741, 879
Eco47I GGWCC 1 cut(s) 383
Eco57I CTGAAG 1 cut(s) 1118
EcoNI CCTNNNNNAGG 1 cut(s) 731
EcoRI GAATTC 1 cut(s) 761
EcoRII CCWGG 2 cut(s) 626, 970
EcoRV GATATC 2 cut(s) 741, 879
Esp3I CGTCTC 2 cut(s) 50, 150
FaeI CATG 9 cut(s) 357, 383, 539, 683, 688, 792, 965, 997, 1003
FaqI GGGAC 1 cut(s) 250
FatI CATG 9 cut(s) 353, 379, 535, 679, 684, 788, 961, 993, 999
FauI CCCGC 1 cut(s) 65
FblI GTMKAC 1 cut(s) 1135
Fnu4HI GCNGC 3 cut(s) 252, 280, 464
FokI GGATG 1 cut(s) 686
Fsp4HI GCNGC 3 cut(s) 252, 280, 464
GluI GCNGC 3 cut(s) 252, 280, 464
HaeIII GGCC 3 cut(s) 267, 808, 931
HgaI GACGC 2 cut(s) 41, 299
Hin1I GRCGYC 2 cut(s) 146, 291
Hin1II CATG 9 cut(s) 357, 383, 539, 683, 688, 792, 965, 997, 1003
HincII GTYRAC 3 cut(s) 144, 853, 1136
HindII GTYRAC 3 cut(s) 144, 853, 1136
HindIII AAGCTT 3 cut(s) 540, 589, 988
HinfI GANTC 3 cut(s) 87, 196, 895
HphI GGTGA 3 cut(s) 77, 241, 735
Hpy166II GTNNAC 6 cut(s) 28, 144, 448, 566, 853, 1136
Hpy188I TCNGA 5 cut(s) 50, 823, 883, 1008, 1190
Hpy188III TCNNGA 8 cut(s) 116, 206, 416, 479, 536, 869, 899, 1065
Hpy8I GTNNAC 6 cut(s) 28, 144, 448, 566, 853, 1136
Hpy99I CGWCG 6 cut(s) 35, 101, 122, 151, 275, 293
HpyAV CCTTC 5 cut(s) 226, 727, 921, 1045, 1093
HpyCH4III ACNGT 2 cut(s) 65, 241
HpyCH4IV ACGT 5 cut(s) 45, 146, 159, 601, 906
HpyCH4V TGCA 6 cut(s) 219, 251, 357, 429, 436, 576
HpyF10VI GCNNNNNNNGC 1 cut(s) 1183
HpyF3I CTNAG 5 cut(s) 83, 587, 945, 1005, 1089
HpySE526I ACGT 5 cut(s) 45, 146, 159, 601, 906
Hsp92I GRCGYC 2 cut(s) 146, 291
Hsp92II CATG 9 cut(s) 357, 383, 539, 683, 688, 792, 965, 997, 1003
KpnI GGTACC 1 cut(s) 43
Kzo9I GATC 8 cut(s) 76, 112, 301, 395, 457, 632, 901, 1170
LguI GCTCTTC 1 cut(s) 576
Lsp1109I GCAGC 2 cut(s) 263, 450
LweI GCATC 4 cut(s) 806, 986, 1135, 1173
MaeII ACGT 5 cut(s) 45, 146, 159, 601, 906
MaeIII GTNAC 3 cut(s) 65, 711, 1149
MalI GATC 8 cut(s) 78, 114, 303, 397, 459, 634, 903, 1172
MboI GATC 8 cut(s) 76, 112, 301, 395, 457, 632, 901, 1170
MboII GAAGA 1 cut(s) 593
MfeI CAATTG 1 cut(s) 261
MflI RGATCY 2 cut(s) 457, 1170
MlsI TGGCCA 1 cut(s) 808
MluCI AATT 4 cut(s) 261, 452, 753, 761
MluI ACGCGT 1 cut(s) 33
MluNI TGGCCA 1 cut(s) 808
MlyI GAGTC 1 cut(s) 96
MmeI TCCRAC 1 cut(s) 132
Mox20I TGGCCA 1 cut(s) 808
MroXI GAANNNNTTC 1 cut(s) 507
MscI TGGCCA 1 cut(s) 808
MseI TTAA 2 cut(s) 401, 638
MslI CAYNNNNRTG 2 cut(s) 755, 998
Msp20I TGGCCA 1 cut(s) 808
MspA1I CMGCKG 1 cut(s) 463
MspR9I CCNGG 2 cut(s) 628, 972
MunI CAATTG 1 cut(s) 261
Mva1269I GAATGC 1 cut(s) 353
MvaI CCWGG 2 cut(s) 628, 972
MvnI CGCG 1 cut(s) 35
MwoI GCNNNNNNNGC 1 cut(s) 1183
NdeII GATC 8 cut(s) 76, 112, 301, 395, 457, 632, 901, 1170
NlaIII CATG 9 cut(s) 357, 383, 539, 683, 688, 792, 965, 997, 1003
NlaIV GGNNCC 3 cut(s) 41, 268, 841
NmuCI GTSAC 1 cut(s) 65
PagI TCATGA 1 cut(s) 535
PciSI GCTCTTC 1 cut(s) 576
PcsI WCGNNNNNNNCGW 1 cut(s) 117
PctI GAATGC 1 cut(s) 353
PdmI GAANNNNTTC 1 cut(s) 507
PfeI GAWTC 2 cut(s) 196, 895
PflFI GACNNNGTC 1 cut(s) 381
PkrI GCNGC 3 cut(s) 253, 281, 465
PleI GAGTC 1 cut(s) 95
PpsI GAGTC 1 cut(s) 95
Psp6I CCWGG 2 cut(s) 626, 970
PspGI CCWGG 2 cut(s) 626, 970
PspN4I GGNNCC 3 cut(s) 41, 268, 841
PspPI GGNCC 2 cut(s) 266, 383
PstI CTGCAG 1 cut(s) 578
PsuI RGATCY 2 cut(s) 457, 1170
PsyI GACNNNGTC 1 cut(s) 381
RsaI GTAC 3 cut(s) 41, 312, 565
RsaNI GTAC 3 cut(s) 40, 311, 564
RseI CAYNNNNRTG 2 cut(s) 755, 998
SalI GTCGAC 1 cut(s) 1134
SapI GCTCTTC 1 cut(s) 576
SaqAI TTAA 2 cut(s) 401, 638
SatI GCNGC 3 cut(s) 252, 280, 464
Sau3AI GATC 8 cut(s) 76, 112, 301, 395, 457, 632, 901, 1170
Sau96I GGNCC 2 cut(s) 266, 383
SchI GAGTC 1 cut(s) 96
ScrFI CCNGG 2 cut(s) 628, 972
SfaNI GCATC 4 cut(s) 806, 986, 1135, 1173
SfcI CTRYAG 1 cut(s) 574
SinI GGWCC 1 cut(s) 383
SmiMI CAYNNNNRTG 2 cut(s) 755, 998
SmlI CTYRAG 1 cut(s) 728
SmoI CTYRAG 1 cut(s) 728
Sse9I AATT 4 cut(s) 261, 452, 753, 761
SsiI CCGC 4 cut(s) 72, 280, 461, 954
SspI AATATT 1 cut(s) 1014
StyD4I CCNGG 2 cut(s) 626, 970
TaaI ACNGT 2 cut(s) 65, 241
TaiI ACGT 5 cut(s) 48, 149, 162, 604, 909
TaqI TCGA 6 cut(s) 79, 176, 194, 394, 980, 1135
TasI AATT 4 cut(s) 261, 452, 753, 761
TatI WGTACW 1 cut(s) 563
TauI GCSGC 1 cut(s) 282
TfiI GAWTC 2 cut(s) 196, 895
Tru1I TTAA 2 cut(s) 401, 638
Tru9I TTAA 2 cut(s) 401, 638
TseFI GTSAC 1 cut(s) 65
TseI GCWGC 2 cut(s) 251, 463
Tsp45I GTSAC 1 cut(s) 65
TspDTI ATGAA 5 cut(s) 352, 552, 690, 754, 982
TspGWI ACGGA 2 cut(s) 74, 506
Tth111I GACNNNGTC 1 cut(s) 381
VpaK11BI GGWCC 1 cut(s) 383
XagI CCTNNNNNAGG 1 cut(s) 731
XapI RAATTY 1 cut(s) 761
XmiI GTMKAC 1 cut(s) 1135
XmnI GAANNNNTTC 1 cut(s) 507
ZraI GACGTC 1 cut(s) 147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.