pycom10g00180

poly(A)-specific ribonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0000405
Physical Location & Seq
Forward (+)
272100 .. 272688
589 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g00180.2

Sequence Viewer

Length: 492 bp
ATGGCGGTCGAGTTCAGGTCTGGCTTGGGCGACGCCTGGTACGACGTCCTGATAGTGAAGGACGGCTGTGTTCGACTGAGAGTGAAGTTCGTCGGCTTCGGCGACGACCATGACGAGGATTATGAAGGTGAGGGAGTTGAGTTCGTTCAACGACATCGACGAGTTCAGGGCACGGAGTGCATCTCTGCCTCCACTAATACAGCGCCGTTTTCTCAGTACCACCACCACCAATGTCTTAAAGAAATGGGCCGTGAAGGAAGTGACGAAGTCCAATTTTCCCGAGTTCCTGGAGGAGTTCAAGAACCACCTGTCCACTTCCGACTTCGTGCCCATGTCGCTTTGACACCGCCGACAAGTCGTATTGCAAGGCTAACTACGCCGCCGAGCGGTTTTAGCTTCTCCAGTTCGCCGCCTGCCCTTTCACTCTCAGAGCCTCCAAGCTTAACCCTTAACCTTCCATACAACTTCGATTGTTCCCAAGGGATGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0043167 GO:0043169
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
CAF1

Protein Analysis

164

Amino Acids

18.13

Weight (kDa)

5.76

Isoelectric Point (pI)

53.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000405)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25330
fragaria_vesca FvH4_2g12920 FvH4_2g12920 FvH4_2g12920 FvH4_2g12920 FvH4_3g17720 FvH4_3g17720 FvH4_3g20410 FvH4_3g20410 FvH4_5g23730 FvH4_5g23730 FvH4_5g23730 FvH4_6g40510 FvH4_6g40520 FvH4_6g40530 FvH4_6g40530
malus_domestica MD09G1128800.v1.1 MD09G1128900.v1.1 MD09G1129100.v1.1 MD17G1118100.v1.1
prunus_persica Prupe.3G196400_v2.0.a1 Prupe.3G196400_v2.0.a1 Prupe.3G196400_v2.0.a1 Prupe.3G196400_v2.0.a1 Prupe.3G196400_v2.0.a1 Prupe.3G196500_v2.0.a1 Prupe.3G196500_v2.0.a1
pyrus_communis pycom09g05230 pycom09g05240 pycom10g00180 pycom17g10860
rosa_chinensis RchiOBHm_Chr2g0133241 RchiOBHm_Chr2g0133311 RchiOBHm_Chr2g0155531 RchiOBHm_Chr2g0155771 RchiOBHm_Chr2g0155781 RchiOBHm_Chr2g0155791 RchiOBHm_Chr5g0029431 RchiOBHm_Chr5g0034301 RchiOBHm_Chr7g0213061
rosa_laevigata RLG00000002835 RLG00000019323 RLG00000020863 RLG00000020864 RLG00000033165 RLG00000033538
rosa_multiflora Rmu_co8060268.1_g000001 Rmu_sc0000062.1_g000021 Rmu_sc0001121.1_g000020 Rmu_sc0001446.1_g000013 Rmu_sc0002712.1_g000021 Rmu_sc0003417.1_g000005 Rmu_sc0023254.1_g000004 Rmu_sc0024719.1_g000001 Rmu_sc0033434.1_g000002
rosa_roxburghii Rroxscaffold_1G00045970 Rroxscaffold_2G00093480 Rroxscaffold_2G00093490 Rroxscaffold_2G00093500 Rroxscaffold_2G00111280 Rroxscaffold_3G00246280 Rroxscaffold_7G00195910
rosa_rugosa Rorug02G0310900 Rorug02G0449800 Rorug02G0449900 Rorug04G0061300 Rorug05G0145100 Rorug06G0068700 Rorug07G0139100
rosa_samantha Rh2AG363400 Rh2AG515900 Rh2AG516000 Rh2BG369100 Rh2BG525000 Rh2BG526900 Rh2BG527000 Rh2CG346700 Rh2CG500900 Rh2CG501000 Rh2DG386100 Rh2DG535900 Rh2DG536000 Rh5AG236700 Rh5BG204800 Rh5BG237200 Rh5CG226800 Rh5CG267000 Rh5DG208400 Rh5DG244700 Rh7AG270400 Rh7AG270500 Rh7BG266000 Rh7CG289700 Rh7CG289800 Rh7DG278700
rosa_wichuraiana Rw2G029570 Rw2G042430 Rw2G042440 Rw5G021290 Rw7G023500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 48
AccBSI CCGCTC 1 cut(s) 387
AciI CCGC 5 cut(s) 5, 347, 380, 387, 410
AcyI GRCGYC 2 cut(s) 33, 45
AdeI CACNNNGTG 1 cut(s) 177
AfaI GTAC 2 cut(s) 41, 218
AfiI CCNNNNNNNGG 2 cut(s) 115, 386
AgsI TTSAA 2 cut(s) 149, 299
AjnI CCWGG 2 cut(s) 35, 286
AloI GAACNNNNNNTCC 2 cut(s) 294, 326
AluBI AGCT 2 cut(s) 396, 441
AluI AGCT 2 cut(s) 396, 441
Ama87I CYCGRG 1 cut(s) 279
AoxI GGCC 1 cut(s) 247
AspLEI GCGC 1 cut(s) 205
AspS9I GGNCC 1 cut(s) 247
AsuHPI GGTGA 1 cut(s) 140
AvaI CYCGRG 1 cut(s) 279
BaeGI GKGCMC 2 cut(s) 173, 331
BceAI ACGGC 3 cut(s) 79, 190, 234
BciT130I CCWGG 2 cut(s) 37, 288
BfoI RGCGCY 1 cut(s) 206
BisI GCNGC 2 cut(s) 380, 410
BlsI GCNGC 2 cut(s) 381, 411
Bme1390I CCNGG 2 cut(s) 37, 288
BmeT110I CYCGRG 1 cut(s) 279
BmgT120I GGNCC 1 cut(s) 247
BmrFI CCNGG 2 cut(s) 37, 288
BmsI GCATC 1 cut(s) 189
BplI GAGNNNNNCTC 2 cut(s) 167, 199
BpmI CTGGAG 2 cut(s) 309, 385
BsaHI GRCGYC 2 cut(s) 33, 45
BsaJI CCNNGG 1 cut(s) 478
Bsc4I CCNNNNNNNGG 2 cut(s) 115, 386
Bse1I ACTGG 1 cut(s) 402
BseBI CCWGG 2 cut(s) 37, 288
BseDI CCNNGG 1 cut(s) 478
BseGI GGATG 1 cut(s) 489
BseLI CCNNNNNNNGG 2 cut(s) 115, 386
BseMII CTCAG 3 cut(s) 68, 227, 441
BseNI ACTGG 1 cut(s) 402
BseRI GAGGAG 1 cut(s) 306
BseSI GKGCMC 2 cut(s) 173, 331
Bsh1285I CGRYCG 1 cut(s) 9
BshFI GGCC 1 cut(s) 249
BsiEI CGRYCG 1 cut(s) 9
BsiHKCI CYCGRG 1 cut(s) 279
BslI CCNNNNNNNGG 2 cut(s) 115, 386
BsnI GGCC 1 cut(s) 249
BsoBI CYCGRG 1 cut(s) 279
Bsp1286I GDGCHC 2 cut(s) 173, 331
BspACI CCGC 5 cut(s) 5, 347, 380, 387, 410
BspANI GGCC 1 cut(s) 249
BspCNI CTCAG 3 cut(s) 69, 226, 440
BsrBI CCGCTC 1 cut(s) 387
BsrI ACTGG 1 cut(s) 402
BssECI CCNNGG 1 cut(s) 478
BssNI GRCGYC 2 cut(s) 33, 45
BssT1I CCWWGG 1 cut(s) 478
Bst2UI CCWGG 2 cut(s) 37, 288
BstACI GRCGYC 2 cut(s) 33, 45
BstAPI GCANNNNNTGC 1 cut(s) 177
BstC8I GCNNGC 1 cut(s) 414
BstDEI CTNAG 3 cut(s) 77, 213, 427
BstF5I GGATG 1 cut(s) 489
BstH2I RGCGCY 1 cut(s) 206
BstHHI GCGC 1 cut(s) 205
BstMCI CGRYCG 1 cut(s) 9
BstMWI GCNNNNNNNGC 4 cut(s) 177, 335, 376, 393
BstNI CCWGG 2 cut(s) 37, 288
BstSCI CCNGG 2 cut(s) 35, 286
BstSLI GKGCMC 2 cut(s) 173, 331
BsuRI GGCC 1 cut(s) 249
BtsCI GGATG 1 cut(s) 489
Cac8I GCNNGC 1 cut(s) 414
CfoI GCGC 1 cut(s) 205
Cfr13I GGNCC 1 cut(s) 247
CseI GACGC 1 cut(s) 41
Csp6I GTAC 2 cut(s) 40, 217
CviAII CATG 2 cut(s) 110, 332
CviJI RGCY 8 cut(s) 24, 66, 96, 249, 370, 396, 433, 441
CviKI_1 RGCY 8 cut(s) 24, 66, 96, 249, 370, 396, 433, 441
CviQI GTAC 2 cut(s) 40, 217
DdeI CTNAG 3 cut(s) 77, 213, 427
DraIII CACNNNGTG 1 cut(s) 177
Eco130I CCWWGG 1 cut(s) 478
Eco88I CYCGRG 1 cut(s) 279
EcoRII CCWGG 2 cut(s) 35, 286
EcoT14I CCWWGG 1 cut(s) 478
ErhI CCWWGG 1 cut(s) 478
FaeI CATG 2 cut(s) 113, 335
FaiI YATR 4 cut(s) 111, 123, 333, 460
FatI CATG 2 cut(s) 109, 331
Fnu4HI GCNGC 2 cut(s) 380, 410
Fsp4HI GCNGC 2 cut(s) 380, 410
GlaI GCGC 1 cut(s) 204
GluI GCNGC 2 cut(s) 380, 410
GsuI CTGGAG 2 cut(s) 309, 385
HaeII RGCGCY 1 cut(s) 206
HaeIII GGCC 1 cut(s) 249
HgaI GACGC 1 cut(s) 41
HhaI GCGC 1 cut(s) 205
Hin1I GRCGYC 2 cut(s) 33, 45
Hin1II CATG 2 cut(s) 113, 335
Hin6I GCGC 1 cut(s) 203
HinP1I GCGC 1 cut(s) 203
HindIII AAGCTT 1 cut(s) 439
HphI GGTGA 1 cut(s) 140
Hpy166II GTNNAC 1 cut(s) 313
Hpy188I TCNGA 2 cut(s) 320, 430
Hpy188III TCNNGA 3 cut(s) 49, 279, 299
Hpy8I GTNNAC 1 cut(s) 313
Hpy99I CGWCG 5 cut(s) 35, 47, 95, 107, 162
HpyAV CCTTC 4 cut(s) 52, 119, 248, 464
HpyCH4IV ACGT 1 cut(s) 45
HpyCH4V TGCA 2 cut(s) 180, 365
HpyF10VI GCNNNNNNNGC 4 cut(s) 177, 335, 376, 393
HpyF3I CTNAG 3 cut(s) 77, 213, 427
HpySE526I ACGT 1 cut(s) 45
Hsp92I GRCGYC 2 cut(s) 33, 45
Hsp92II CATG 2 cut(s) 113, 335
HspAI GCGC 1 cut(s) 203
LweI GCATC 1 cut(s) 189
MaeII ACGT 1 cut(s) 45
MaeIII GTNAC 1 cut(s) 260
MbiI CCGCTC 1 cut(s) 387
MhlI GDGCHC 2 cut(s) 173, 331
MluCI AATT 1 cut(s) 272
MmeI TCCRAC 1 cut(s) 343
MnlI CCTC 5 cut(s) 109, 124, 199, 284, 444
MseI TTAA 3 cut(s) 237, 443, 450
MspR9I CCNGG 2 cut(s) 37, 288
MvaI CCWGG 2 cut(s) 37, 288
MwoI GCNNNNNNNGC 4 cut(s) 177, 335, 376, 393
NlaIII CATG 2 cut(s) 113, 335
NmeAIII GCCGAG 1 cut(s) 408
NmuCI GTSAC 1 cut(s) 260
PcsI WCGNNNNNNNCGW 4 cut(s) 39, 99, 111, 157
PflFI GACNNNGTC 1 cut(s) 266
PfoI TCCNGGA 1 cut(s) 286
PkrI GCNGC 2 cut(s) 381, 411
Psp6I CCWGG 2 cut(s) 35, 286
PspGI CCWGG 2 cut(s) 35, 286
PspPI GGNCC 1 cut(s) 247
PsyI GACNNNGTC 1 cut(s) 266
RsaI GTAC 2 cut(s) 41, 218
RsaNI GTAC 2 cut(s) 40, 217
SaqAI TTAA 3 cut(s) 237, 443, 450
SatI GCNGC 2 cut(s) 380, 410
Sau96I GGNCC 1 cut(s) 247
ScrFI CCNGG 2 cut(s) 37, 288
SduI GDGCHC 2 cut(s) 173, 331
SetI ASST 7 cut(s) 20, 48, 130, 310, 398, 443, 456
SfaNI GCATC 1 cut(s) 189
Sse9I AATT 1 cut(s) 272
SsiI CCGC 5 cut(s) 5, 347, 380, 387, 410
StyD4I CCNGG 2 cut(s) 35, 286
StyI CCWWGG 1 cut(s) 478
TaiI ACGT 1 cut(s) 48
TaqI TCGA 4 cut(s) 9, 73, 157, 468
TasI AATT 1 cut(s) 272
TauI GCSGC 2 cut(s) 382, 412
Tru1I TTAA 3 cut(s) 237, 443, 450
Tru9I TTAA 3 cut(s) 237, 443, 450
TseFI GTSAC 1 cut(s) 260
Tsp45I GTSAC 1 cut(s) 260
TspDTI ATGAA 1 cut(s) 138
TspGWI ACGGA 1 cut(s) 188
Tth111I GACNNNGTC 1 cut(s) 266
ZraI GACGTC 1 cut(s) 46
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.