Rroxscaffold_2G00122870

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0015773
Physical Location & Seq
Forward (+)
56292748 .. 56298119
5372 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00122870.1

Sequence Viewer

Length: 435 bp
ATGGGTCGGGCCGAGGGCGTCTTCCATGGGGCGAAGCCGGAGGTTATGCTCGTAACGGTGGCATGGTGGTCGACGGCGCAGGACTGCAGGGATGCTAGGGATTCTCAGGTTTCCTACTCGGTCAATGGTTTGGAGTATTTGGGCCAATTGGGCCTCAAACTGGATGGTGAGCCTCATCCTTGGGCTTGGTCCGGGGTTATGCAAGGCTTGGACTACCTCTTTGCTAGGGAGTTGGATGGAAGTATAGGGGGAAACAAGGGGAAGAAAAGAGAGGCGCTGCCTGAGAATTGCCCAGAAAGAACAAAAGAGGAGAGAGATAGAGCGATGTCGGCGACTCTTGAGAAGAGAATACAGATGGAGTGGGGTGTACTAGAGTGGACCACGCTGCAAAAGTATTATGAAGAAAAATTGGATACACACCAACACACAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

144

Amino Acids

16.46

Weight (kDa)

5.6

Isoelectric Point (pI)

22.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 71
AcyI GRCGYC 1 cut(s) 18
AdeI CACNNNGTG 1 cut(s) 432
AfaI GTAC 1 cut(s) 369
AfiI CCNNNNNNNGG 1 cut(s) 160
AoxI GGCC 3 cut(s) 9, 142, 151
ApeKI GCWGC 2 cut(s) 277, 385
ArsI GACNNNNNNTTYG 2 cut(s) 203, 235
AspLEI GCGC 2 cut(s) 79, 277
AspS9I GGNCC 5 cut(s) 9, 142, 151, 189, 378
AsuC2I CCSGG 1 cut(s) 193
AsuHPI GGTGA 1 cut(s) 179
AvaII GGWCC 2 cut(s) 189, 378
BbsI GAAGAC 1 cut(s) 13
BbvI GCAGC 2 cut(s) 264, 372
BccI CCATC 3 cut(s) 158, 230, 349
BceAI ACGGC 1 cut(s) 90
BcgI CGANNNNNNTGC 2 cut(s) 51, 85
BciVI GTATCC 1 cut(s) 406
BcnI CCSGG 1 cut(s) 193
BfaI CTAG 3 cut(s) 96, 225, 371
BfmI CTRYAG 1 cut(s) 85
BfoI RGCGCY 1 cut(s) 278
BfuI GTATCC 1 cut(s) 406
BglI GCCNNNNNGGC 1 cut(s) 150
BisI GCNGC 2 cut(s) 278, 386
BlsI GCNGC 2 cut(s) 279, 387
Bme1390I CCNGG 1 cut(s) 193
Bme18I GGWCC 2 cut(s) 189, 378
BmgT120I GGNCC 5 cut(s) 9, 142, 151, 189, 378
BmrFI CCNGG 1 cut(s) 193
BmsI GCATC 1 cut(s) 82
BpiI GAAGAC 1 cut(s) 13
BpuEI CTTGAG 1 cut(s) 359
BpuMI CCSGG 1 cut(s) 193
BsaHI GRCGYC 1 cut(s) 18
BsaJI CCNNGG 4 cut(s) 12, 25, 179, 192
Bsc4I CCNNNNNNNGG 1 cut(s) 160
Bse1I ACTGG 1 cut(s) 165
BseDI CCNNGG 4 cut(s) 12, 25, 179, 192
BseGI GGATG 4 cut(s) 97, 169, 175, 241
BseLI CCNNNNNNNGG 1 cut(s) 160
BseMII CTCAG 2 cut(s) 119, 273
BseNI ACTGG 1 cut(s) 165
BseRI GAGGAG 1 cut(s) 323
BseXI GCAGC 2 cut(s) 264, 372
BshFI GGCC 3 cut(s) 11, 144, 153
BsiSI CCGG 2 cut(s) 38, 192
BslI CCNNNNNNNGG 1 cut(s) 160
BsnI GGCC 3 cut(s) 11, 144, 153
Bsp19I CCATGG 1 cut(s) 25
BspANI GGCC 3 cut(s) 11, 144, 153
BspCNI CTCAG 2 cut(s) 118, 274
BspMAI CTGCAG 1 cut(s) 89
BsrI ACTGG 1 cut(s) 165
BssECI CCNNGG 4 cut(s) 12, 25, 179, 192
BssNI GRCGYC 1 cut(s) 18
BssT1I CCWWGG 2 cut(s) 25, 179
Bst4CI ACNGT 1 cut(s) 58
Bst6I CTCTTC 1 cut(s) 338
BstACI GRCGYC 1 cut(s) 18
BstDEI CTNAG 2 cut(s) 105, 282
BstDSI CCRYGG 1 cut(s) 25
BstF5I GGATG 4 cut(s) 97, 169, 175, 241
BstH2I RGCGCY 1 cut(s) 278
BstHHI GCGC 2 cut(s) 79, 277
BstMWI GCNNNNNNNGC 2 cut(s) 150, 329
BstSCI CCNGG 1 cut(s) 191
BstSFI CTRYAG 1 cut(s) 85
BstV1I GCAGC 2 cut(s) 264, 372
BstV2I GAAGAC 1 cut(s) 13
BsuI GTATCC 1 cut(s) 406
BsuRI GGCC 3 cut(s) 11, 144, 153
BtgI CCRYGG 1 cut(s) 25
BtgZI GCGATG 1 cut(s) 338
BtsCI GGATG 4 cut(s) 97, 169, 175, 241
CfoI GCGC 2 cut(s) 79, 277
Cfr13I GGNCC 5 cut(s) 9, 142, 151, 189, 378
CseI GACGC 1 cut(s) 7
Csp6I GTAC 1 cut(s) 368
CviAII CATG 2 cut(s) 26, 63
CviJI RGCY 7 cut(s) 11, 37, 144, 153, 172, 185, 207
CviKI_1 RGCY 7 cut(s) 11, 37, 144, 153, 172, 185, 207
CviQI GTAC 1 cut(s) 368
DdeI CTNAG 2 cut(s) 105, 282
DraIII CACNNNGTG 1 cut(s) 432
Eam1104I CTCTTC 1 cut(s) 338
EarI CTCTTC 1 cut(s) 338
Eco130I CCWWGG 2 cut(s) 25, 179
Eco47I GGWCC 2 cut(s) 189, 378
EcoT14I CCWWGG 2 cut(s) 25, 179
ErhI CCWWGG 2 cut(s) 25, 179
FaeI CATG 2 cut(s) 29, 66
FaiI YATR 6 cut(s) 27, 47, 64, 200, 245, 399
FatI CATG 2 cut(s) 25, 62
FblI GTMKAC 1 cut(s) 71
Fnu4HI GCNGC 2 cut(s) 278, 386
FokI GGATG 4 cut(s) 104, 162, 176, 248
Fsp4HI GCNGC 2 cut(s) 278, 386
FspBI CTAG 3 cut(s) 96, 225, 371
GlaI GCGC 2 cut(s) 78, 276
GluI GCNGC 2 cut(s) 278, 386
HaeII RGCGCY 1 cut(s) 278
HaeIII GGCC 3 cut(s) 11, 144, 153
HapII CCGG 2 cut(s) 38, 192
HgaI GACGC 1 cut(s) 7
HhaI GCGC 2 cut(s) 79, 277
Hin1I GRCGYC 1 cut(s) 18
Hin1II CATG 2 cut(s) 29, 66
Hin6I GCGC 2 cut(s) 77, 275
HinP1I GCGC 2 cut(s) 77, 275
HincII GTYRAC 1 cut(s) 72
HindII GTYRAC 1 cut(s) 72
HinfI GANTC 2 cut(s) 101, 334
HpaII CCGG 2 cut(s) 38, 192
HphI GGTGA 1 cut(s) 179
Hpy166II GTNNAC 3 cut(s) 72, 368, 378
Hpy188III TCNNGA 1 cut(s) 338
Hpy8I GTNNAC 3 cut(s) 72, 368, 378
Hpy99I CGWCG 1 cut(s) 76
HpyCH4III ACNGT 1 cut(s) 58
HpyCH4V TGCA 3 cut(s) 87, 202, 388
HpyF10VI GCNNNNNNNGC 2 cut(s) 150, 329
HpyF3I CTNAG 2 cut(s) 105, 282
Hsp92I GRCGYC 1 cut(s) 18
Hsp92II CATG 2 cut(s) 29, 66
HspAI GCGC 2 cut(s) 77, 275
LpnPI CCDG 8 cut(s) 51, 65, 73, 92, 146, 205, 294, 306
Lsp1109I GCAGC 2 cut(s) 264, 372
LweI GCATC 1 cut(s) 82
MaeI CTAG 3 cut(s) 96, 225, 371
MaeIII GTNAC 1 cut(s) 52
MboII GAAGA 4 cut(s) 13, 274, 355, 413
MfeI CAATTG 1 cut(s) 146
MluCI AATT 3 cut(s) 146, 286, 407
MlyI GAGTC 1 cut(s) 328
MmeI TCCRAC 1 cut(s) 213
MnlI CCTC 7 cut(s) 7, 34, 164, 183, 227, 265, 301
MspI CCGG 2 cut(s) 38, 192
MspR9I CCNGG 1 cut(s) 193
MunI CAATTG 1 cut(s) 146
MwoI GCNNNNNNNGC 2 cut(s) 150, 329
NciI CCSGG 1 cut(s) 193
NcoI CCATGG 1 cut(s) 25
NlaIII CATG 2 cut(s) 29, 66
NmeAIII GCCGAG 1 cut(s) 37
PfeI GAWTC 1 cut(s) 101
PkrI GCNGC 2 cut(s) 279, 387
PleI GAGTC 1 cut(s) 328
PpsI GAGTC 1 cut(s) 328
PspPI GGNCC 5 cut(s) 9, 142, 151, 189, 378
PstI CTGCAG 1 cut(s) 89
RsaI GTAC 1 cut(s) 369
RsaNI GTAC 1 cut(s) 368
SalI GTCGAC 1 cut(s) 70
SatI GCNGC 2 cut(s) 278, 386
Sau96I GGNCC 5 cut(s) 9, 142, 151, 189, 378
SchI GAGTC 1 cut(s) 328
ScrFI CCNGG 1 cut(s) 193
SetI ASST 3 cut(s) 45, 111, 219
SfaNI GCATC 1 cut(s) 82
SfcI CTRYAG 1 cut(s) 85
SfiI GGCCNNNNNGGCC 1 cut(s) 150
SinI GGWCC 2 cut(s) 189, 378
SmlI CTYRAG 1 cut(s) 338
SmoI CTYRAG 1 cut(s) 338
Sse9I AATT 3 cut(s) 146, 286, 407
SspMI CTAG 3 cut(s) 96, 225, 371
StyD4I CCNGG 1 cut(s) 191
StyI CCWWGG 2 cut(s) 25, 179
TaaI ACNGT 1 cut(s) 58
TaqI TCGA 1 cut(s) 71
TaqII GACCGA 1 cut(s) 109
TasI AATT 3 cut(s) 146, 286, 407
TatI WGTACW 1 cut(s) 367
TfiI GAWTC 1 cut(s) 101
TseI GCWGC 2 cut(s) 277, 385
TspDTI ATGAA 1 cut(s) 414
VpaK11BI GGWCC 2 cut(s) 189, 378
XmiI GTMKAC 1 cut(s) 71
XspI CTAG 3 cut(s) 96, 225, 371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.