Rroxscaffold_2G00144220

Thiol-disulfide oxidoreductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0010979
Physical Location & Seq
Forward (+)
82141526 .. 82144321
2796 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00144220.1

Sequence Viewer

Length: 1125 bp
ATGGCGACCTTGGCGCTCCTGAGCCTAACATCTCCTTCACTCTCCTCTCCCAGATCTCTTCCTCGCTCAAATCACACCAGATTCGGTGTCCTTACTACCACTTCGGGTTGGGCTCGGAGAACTGTGCGGCTTCCAGTTCTATGCTCAGCTTCGGAGCCCAATCAAAACGCTGAATCCGAACCGGAAGCGACGTCGTCTCCGCCATCGTCTTCTTCGAGTGACTTGACCTACAAACTATGTGCCGGGCTCGGAGGCATCGGGTTTCTCGAAACGACCTACTTGACCTTCCTCAAGCTCACGAATTCCGATGCCTTCTGCCCCACCGGCGGCGGCGGCGGCGGCAGTTGCGGCGACATCCTCAACAGTGAATACGCCGTCGTTTTTGGGGTTCCTCTTCCGTTGTTTGGAATGCTTGCGTATGGGTTAGTTGCTACACTTGGTGTGCAGCTGTTGACTGCAAAGAGTTTACCTTTTGGAATTGGGAAATCTGATGCTGGCTTGGTTTTACTTGGAACTACAACCTCCATGGCAGCTGCTAGTGCTTGTTTCTTGTACGTTCTTAGCACACAGTTTTCGGGAACTTCGTGTTCGTATTGTTTGTTATCGGCTTTCCTCTCCTTCAGCTTATTTTTCATCACTTTAAAGGATTTTGGGTTCGAAAAAGTACAAAAAGAGGCGGGTTTATTGGTGTGTATAGCTGTCTTGGTGGCTGTGACTCTGAACAGGTCATATAGTGCACCTGTATCCTCAAGCCCATCTGATGTTGATCTTCCATACTTTACAACTGAGATAACAACACCATCAAGTCCGTTTGCCATATCTCTTGCAAAGCATCTAAATGCTATAGGTGCTAAAATGTATGGCGCTTTTTGGTGTTCACATTGTGTTGAACAAAAACAGATGTTTGGACGTGAAGCAGCGAAGGAATTGAACTATGTTGAGTGCTTCCCTGGTGGATTTAGAAAGGGAACTATAATGGCAAAGGCATGTATTGATGCCAAACTTGAAGGTTTTCCTACTTGGGTCATCAATGGACAGGTTTTGAGTGGAGAAAAAGATTTGCTAGAGCTTGCAGAGGCTTCTGGGTTTGAGTTGAACGAATTTAGTCAGACCCTCATAGAGTAA

Protein Analysis

374

Amino Acids

39.54

Weight (kDa)

5.39

Isoelectric Point (pI)

46.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VKOR PF07884 79 - 213 1.2e-24 Vitamin K epoxide reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010979)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G35760 AT4G35760
fragaria_vesca FvH4_1g10920 FvH4_1g10920
malus_domestica MD02G1122300.v1.1 MD15G1236200.v1.1
prunus_persica Prupe.7G176300_v2.0.a1
pyrus_communis pycom02g09520 pycom15g21020
rosa_chinensis RchiOBHm_Chr2g0098181
rosa_laevigata RLG00000016769
rosa_multiflora Rmu_sc0009178.1_g000009 Rmu_sc0023861.1_g000003
rosa_roxburghii Rroxscaffold_2G00144220
rosa_rugosa Rorug02G0070800
rosa_samantha Rh2AG118100 Rh2BG121100 Rh2CG122700 Rh2DG122600
rosa_wichuraiana Rw2G009240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 194
AciI CCGC 9 cut(s) 127, 200, 327, 330, 333, 336, 339, 348, 677
AcsI RAATTY 2 cut(s) 301, 1100
AcuI CTGAAG 1 cut(s) 604
AcyI GRCGYC 1 cut(s) 191
AdeI CACNNNGTG 2 cut(s) 440, 884
AfaI GTAC 2 cut(s) 554, 666
AfiI CCNNNNNNNGG 2 cut(s) 326, 404
AgsI TTSAA 4 cut(s) 890, 931, 1007, 1096
AjiI CACGTC 1 cut(s) 911
AjnI CCWGG 1 cut(s) 949
AloI GAACNNNNNNTCC 2 cut(s) 638, 670
AluBI AGCT 7 cut(s) 149, 295, 448, 533, 624, 698, 1069
AluI AGCT 7 cut(s) 149, 295, 448, 533, 624, 698, 1069
Alw21I GWGCWC 1 cut(s) 739
Alw26I GTCTC 1 cut(s) 201
Alw44I GTGCAC 1 cut(s) 735
ApaLI GTGCAC 1 cut(s) 735
ApeKI GCWGC 4 cut(s) 445, 530, 533, 917
ApoI RAATTY 2 cut(s) 301, 1100
Asp700I GAANNNNTTC 1 cut(s) 1011
AspLEI GCGC 2 cut(s) 16, 866
AsuC2I CCSGG 1 cut(s) 244
AsuII TTCGAA 1 cut(s) 657
BaeGI GKGCMC 1 cut(s) 739
BanII GRGCYC 3 cut(s) 115, 159, 249
BbsI GAAGAC 1 cut(s) 201
Bbv12I GWGCWC 1 cut(s) 739
BbvI GCAGC 4 cut(s) 457, 520, 542, 929
BccI CCATC 3 cut(s) 211, 763, 808
BceAI ACGGC 1 cut(s) 359
BciT130I CCWGG 1 cut(s) 951
BciVI GTATCC 1 cut(s) 754
BcnI CCSGG 1 cut(s) 244
BcoDI GTCTC 1 cut(s) 201
BfaI CTAG 2 cut(s) 537, 1064
BfmI CTRYAG 1 cut(s) 843
BfoI RGCGCY 2 cut(s) 17, 867
BfuI GTATCC 1 cut(s) 754
BglI GCCNNNNNGGC 1 cut(s) 324
BglII AGATCT 1 cut(s) 53
BlpI GCTNAGC 1 cut(s) 145
Bme1390I CCNGG 2 cut(s) 244, 951
BmgBI CACGTC 1 cut(s) 911
BmiI GGNNCC 2 cut(s) 156, 390
BmrFI CCNGG 2 cut(s) 244, 951
BmsI GCATC 5 cut(s) 264, 298, 481, 841, 985
BpiI GAAGAC 1 cut(s) 201
Bpu10I CCTNAGC 1 cut(s) 20
Bpu1102I GCTNAGC 1 cut(s) 145
Bpu14I TTCGAA 1 cut(s) 657
BpuEI CTTGAG 2 cut(s) 275, 733
BpuMI CCSGG 1 cut(s) 244
BsaBI GATNNNNATC 1 cut(s) 765
BsaHI GRCGYC 1 cut(s) 191
BsaJI CCNNGG 3 cut(s) 9, 525, 949
BsaWI WCCGGW 1 cut(s) 181
BsaXI ACNNNNNCTCC 2 cut(s) 181, 211
Bsc4I CCNNNNNNNGG 2 cut(s) 326, 404
Bse118I RCCGGY 1 cut(s) 323
Bse1I ACTGG 1 cut(s) 134
Bse8I GATNNNNATC 1 cut(s) 765
BseBI CCWGG 1 cut(s) 951
BseDI CCNNGG 3 cut(s) 9, 525, 949
BseGI GGATG 1 cut(s) 354
BseJI GATNNNNATC 1 cut(s) 765
BseLI CCNNNNNNNGG 2 cut(s) 326, 404
BseMII CTCAG 3 cut(s) 11, 159, 777
BseNI ACTGG 1 cut(s) 134
BseRI GAGGAG 1 cut(s) 34
BseSI GKGCMC 1 cut(s) 739
BseXI GCAGC 4 cut(s) 457, 520, 542, 929
BsgI GTGCAG 1 cut(s) 464
BsiHKAI GWGCWC 1 cut(s) 739
BsiSI CCGG 3 cut(s) 182, 243, 324
BslI CCNNNNNNNGG 2 cut(s) 326, 404
BsmAI GTCTC 1 cut(s) 201
BsmBI CGTCTC 1 cut(s) 201
BsmI GAATGC 1 cut(s) 414
Bsp119I TTCGAA 1 cut(s) 657
Bsp1286I GDGCHC 4 cut(s) 115, 159, 249, 739
Bsp143I GATC 2 cut(s) 53, 766
Bsp1720I GCTNAGC 1 cut(s) 145
Bsp19I CCATGG 1 cut(s) 525
BspACI CCGC 9 cut(s) 127, 200, 327, 330, 333, 336, 339, 348, 677
BspCNI CTCAG 3 cut(s) 12, 158, 778
BspLI GGNNCC 2 cut(s) 156, 390
BspT104I TTCGAA 1 cut(s) 657
BsrFI RCCGGY 1 cut(s) 323
BsrI ACTGG 1 cut(s) 134
BssAI RCCGGY 1 cut(s) 323
BssECI CCNNGG 3 cut(s) 9, 525, 949
BssMI GATC 2 cut(s) 53, 766
BssNI GRCGYC 1 cut(s) 191
BssT1I CCWWGG 2 cut(s) 9, 525
Bst2UI CCWGG 1 cut(s) 951
Bst4CI ACNGT 3 cut(s) 124, 365, 570
Bst6I CTCTTC 2 cut(s) 63, 399
BstACI GRCGYC 1 cut(s) 191
BstBI TTCGAA 1 cut(s) 657
BstC8I GCNNGC 3 cut(s) 414, 496, 1071
BstDEI CTNAG 4 cut(s) 20, 145, 560, 786
BstDSI CCRYGG 1 cut(s) 525
BstF5I GGATG 1 cut(s) 354
BstH2I RGCGCY 2 cut(s) 17, 867
BstHHI GCGC 2 cut(s) 16, 866
BstKTI GATC 2 cut(s) 56, 769
BstMAI GTCTC 1 cut(s) 201
BstMBI GATC 2 cut(s) 53, 766
BstMWI GCNNNNNNNGC 9 cut(s) 11, 324, 333, 336, 339, 345, 348, 539, 848
BstNI CCWGG 1 cut(s) 951
BstNSI RCATGY 1 cut(s) 990
BstSCI CCNGG 2 cut(s) 242, 949
BstSFI CTRYAG 1 cut(s) 843
BstSLI GKGCMC 1 cut(s) 739
BstV1I GCAGC 4 cut(s) 457, 520, 542, 929
BstV2I GAAGAC 1 cut(s) 201
BstX2I RGATCY 1 cut(s) 53
BstYI RGATCY 1 cut(s) 53
BsuI GTATCC 1 cut(s) 754
BtgI CCRYGG 1 cut(s) 525
BtrI CACGTC 1 cut(s) 911
BtsCI GGATG 1 cut(s) 354
BtsIMutI CAGTG 1 cut(s) 370
Cac8I GCNNGC 3 cut(s) 414, 496, 1071
CfoI GCGC 2 cut(s) 16, 866
Cfr10I RCCGGY 1 cut(s) 323
Csp6I GTAC 2 cut(s) 553, 665
CviAII CATG 2 cut(s) 526, 987
CviQI GTAC 2 cut(s) 553, 665
DdeI CTNAG 4 cut(s) 20, 145, 560, 786
DpnI GATC 2 cut(s) 55, 768
DpnII GATC 2 cut(s) 53, 766
DraI TTTAAA 1 cut(s) 642
DraIII CACNNNGTG 2 cut(s) 440, 884
Eam1104I CTCTTC 2 cut(s) 63, 399
EarI CTCTTC 2 cut(s) 63, 399
EciI GGCGGA 1 cut(s) 189
Eco130I CCWWGG 2 cut(s) 9, 525
Eco24I GRGCYC 3 cut(s) 115, 159, 249
Eco57I CTGAAG 1 cut(s) 604
EcoRI GAATTC 1 cut(s) 301
EcoRII CCWGG 1 cut(s) 949
EcoT14I CCWWGG 2 cut(s) 9, 525
EcoT38I GRGCYC 3 cut(s) 115, 159, 249
ErhI CCWWGG 2 cut(s) 9, 525
Esp3I CGTCTC 1 cut(s) 201
FaeI CATG 2 cut(s) 529, 990
FatI CATG 2 cut(s) 525, 986
FauI CCCGC 1 cut(s) 670
FokI GGATG 1 cut(s) 341
FriOI GRGCYC 3 cut(s) 115, 159, 249
FspBI CTAG 2 cut(s) 537, 1064
GlaI GCGC 2 cut(s) 15, 865
HaeII RGCGCY 2 cut(s) 17, 867
HapII CCGG 3 cut(s) 182, 243, 324
HhaI GCGC 2 cut(s) 16, 866
Hin1I GRCGYC 1 cut(s) 191
Hin1II CATG 2 cut(s) 529, 990
Hin6I GCGC 2 cut(s) 14, 864
HinP1I GCGC 2 cut(s) 14, 864
HincII GTYRAC 1 cut(s) 453
HindII GTYRAC 1 cut(s) 453
HinfI GANTC 3 cut(s) 81, 173, 715
HpaII CCGG 3 cut(s) 182, 243, 324
Hpy166II GTNNAC 4 cut(s) 453, 467, 737, 878
Hpy188I TCNGA 9 cut(s) 117, 154, 178, 251, 307, 490, 720, 760, 1110
Hpy188III TCNNGA 4 cut(s) 19, 266, 298, 576
Hpy8I GTNNAC 4 cut(s) 453, 467, 737, 878
Hpy99I CGWCG 3 cut(s) 193, 196, 380
HpyAV CCTTC 6 cut(s) 45, 295, 322, 628, 916, 1001
HpyCH4III ACNGT 3 cut(s) 124, 365, 570
HpyCH4IV ACGT 3 cut(s) 191, 555, 910
HpyCH4V TGCA 5 cut(s) 445, 458, 737, 827, 1073
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 324, 333, 336, 339, 345, 348, 539, 848
HpyF3I CTNAG 4 cut(s) 20, 145, 560, 786
HpySE526I ACGT 3 cut(s) 191, 555, 910
Hsp92I GRCGYC 1 cut(s) 191
Hsp92II CATG 2 cut(s) 529, 990
HspAI GCGC 2 cut(s) 14, 864
Kzo9I GATC 2 cut(s) 53, 766
LmnI GCTCC 2 cut(s) 21, 154
Lsp1109I GCAGC 4 cut(s) 457, 520, 542, 929
LweI GCATC 5 cut(s) 264, 298, 481, 841, 985
MaeI CTAG 2 cut(s) 537, 1064
MaeII ACGT 3 cut(s) 191, 555, 910
MaeIII GTNAC 2 cut(s) 218, 712
MalI GATC 2 cut(s) 55, 768
MboI GATC 2 cut(s) 53, 766
MboII GAAGA 5 cut(s) 50, 201, 204, 386, 761
MflI RGATCY 1 cut(s) 53
MhlI GDGCHC 4 cut(s) 115, 159, 249, 739
MluCI AATT 4 cut(s) 301, 477, 926, 1100
MlyI GAGTC 1 cut(s) 709
MroXI GAANNNNTTC 1 cut(s) 1011
MseI TTAA 1 cut(s) 641
MslI CAYNNNNRTG 1 cut(s) 837
MspA1I CMGCKG 2 cut(s) 448, 533
MspI CCGG 3 cut(s) 182, 243, 324
MspR9I CCNGG 2 cut(s) 244, 951
Mva1269I GAATGC 1 cut(s) 414
MvaI CCWGG 1 cut(s) 951
MwoI GCNNNNNNNGC 9 cut(s) 11, 324, 333, 336, 339, 345, 348, 539, 848
NciI CCSGG 1 cut(s) 244
NcoI CCATGG 1 cut(s) 525
NdeII GATC 2 cut(s) 53, 766
NlaIII CATG 2 cut(s) 529, 990
NlaIV GGNNCC 2 cut(s) 156, 390
NmuCI GTSAC 2 cut(s) 218, 712
NspI RCATGY 1 cut(s) 990
NspV TTCGAA 1 cut(s) 657
PcsI WCGNNNNNNNCGW 4 cut(s) 174, 212, 264, 581
PctI GAATGC 1 cut(s) 414
PdmI GAANNNNTTC 1 cut(s) 1011
PfeI GAWTC 2 cut(s) 81, 173
PflFI GACNNNGTC 1 cut(s) 193
PleI GAGTC 1 cut(s) 709
PpsI GAGTC 1 cut(s) 709
Psp6I CCWGG 1 cut(s) 949
PspGI CCWGG 1 cut(s) 949
PspN4I GGNNCC 2 cut(s) 156, 390
PsuI RGATCY 1 cut(s) 53
PsyI GACNNNGTC 1 cut(s) 193
PvuII CAGCTG 2 cut(s) 448, 533
RsaI GTAC 2 cut(s) 554, 666
RsaNI GTAC 2 cut(s) 553, 665
RseI CAYNNNNRTG 1 cut(s) 837
SaqAI TTAA 1 cut(s) 641
Sau3AI GATC 2 cut(s) 53, 766
SchI GAGTC 1 cut(s) 709
ScrFI CCNGG 2 cut(s) 244, 951
SduI GDGCHC 4 cut(s) 115, 159, 249, 739
SfaNI GCATC 5 cut(s) 264, 298, 481, 841, 985
SfcI CTRYAG 1 cut(s) 843
SfuI TTCGAA 1 cut(s) 657
SgrAI CRCCGGYG 1 cut(s) 323
SmiMI CAYNNNNRTG 1 cut(s) 837
SmlI CTYRAG 2 cut(s) 290, 748
SmoI CTYRAG 2 cut(s) 290, 748
Sse9I AATT 4 cut(s) 301, 477, 926, 1100
SsiI CCGC 9 cut(s) 127, 200, 327, 330, 333, 336, 339, 348, 677
SspMI CTAG 2 cut(s) 537, 1064
StyD4I CCNGG 2 cut(s) 242, 949
StyI CCWWGG 2 cut(s) 9, 525
TaaI ACNGT 3 cut(s) 124, 365, 570
TaiI ACGT 3 cut(s) 194, 558, 913
TaqI TCGA 3 cut(s) 215, 267, 657
TasI AATT 4 cut(s) 301, 477, 926, 1100
TatI WGTACW 1 cut(s) 664
TauI GCSGC 7 cut(s) 130, 330, 333, 336, 339, 342, 351
TfiI GAWTC 2 cut(s) 81, 173
Tru1I TTAA 1 cut(s) 641
Tru9I TTAA 1 cut(s) 641
TscAI CASTG 1 cut(s) 370
TseFI GTSAC 2 cut(s) 218, 712
TseI GCWGC 4 cut(s) 445, 530, 533, 917
Tsp45I GTSAC 2 cut(s) 218, 712
TspDTI ATGAA 1 cut(s) 622
TspGWI ACGGA 2 cut(s) 387, 798
TspRI CASTG 1 cut(s) 370
Tth111I GACNNNGTC 1 cut(s) 193
VneI GTGCAC 1 cut(s) 735
XapI RAATTY 2 cut(s) 301, 1100
XceI RCATGY 1 cut(s) 990
XmnI GAANNNNTTC 1 cut(s) 1011
XspI CTAG 2 cut(s) 537, 1064
ZraI GACGTC 1 cut(s) 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.