Rh2DG122600

Thiol-disulfide oxidoreductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0010979
Physical Location & Seq
Reverse (-)
10209218 .. 10212091
2874 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG122600.1

Sequence Viewer

Length: 1098 bp
ATGGCTACCTTGGCGCTCCTGAGCCTAACATCTCCTTCACTCTCCTCTCCCAGATCTCTTCCTCGCTCAAATCACACCAGATTCGGTGCCCTTACTACCACTTCGGGTTGGGCTCGGAGAACTGTGCGGCTTCCCGTTCTATGCTCAGCTTCGGAGCCCAATCAAAACGCCGAATCCGAACCGGAAACGACGTCGTCTCCGCCGTCGTCTTCGAGTGACTTGACCTACAAACTATGTGCCGGGCTCGGAGGCATCGGGTTTCTCGAAACGACCTACTTGACCTTCCTCAAGCTCACGAATTCCGATGCCTTCTGCCCCACCGGCGCCGGCGGCGGAAGTTGCGGCGACATCCTCAACAGTGAATACGCCGTCGTTTTTGGGGTTCCTCTTCCGTTGTTTGGAATGCTTGCATATGGGTTAGTTGCTACACTTGGTGTGCAGCTGTTGACTGCAAAGAGTTTACCTTTTGGAATTGGGAAATCTGATGCTAGCTTGGTTTTACTTGGGACTACGACCTCCATGGCAGCTGCTAGTGCTTGTTTTTTGTACATTCTTAGCACACAGTTTTCGGGAACTTCGTGTTCGTATTGTTTGTTATCGGCTTTCCTCTCCTTCAGCTTATTTTTCATCACTTTAAAGGATTTTGGGTTCGAAAAAGTACAAAAAGAGGCGGGTTTATTGGTGTGTATAGCTGTCTTGGTGGCTGTGACTCTGAACAGGTCATATAGTGCACCTGTATCCTCAAGCCCATCTGATGTTGATCTTCCATACTTTACAACTGAGATAACAACACCATCAAGTCCGTTTGCTATATCTCTTGCAAAGCATCTAAATGCTATAGGTGCTAAAATGTATGGCGCTTTTTGGTGTTCACATTGTGTTGAACAAAAACAGATGTTTGGACGTGAAGCAGCGAAGGAATTGAACTATGTTGAGTGCTTCCCTGGTGGATTTAGAAAGGGAACTATAATGGCAAAGGCATGTATTGATGCCAAACTTGAAGGTTTTCCTACTTGGGTCATCAATGGACAGGTGATAAATCTCATCATAGATTCTGGAACATGGTCATTCACTCATTCGGTTCATCCTTATTGTTGA

Protein Analysis

365

Amino Acids

38.76

Weight (kDa)

6.92

Isoelectric Point (pI)

44.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VKOR PF07884 78 - 211 7.1e-25 Vitamin K epoxide reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010979)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G35760 AT4G35760
fragaria_vesca FvH4_1g10920 FvH4_1g10920
malus_domestica MD02G1122300.v1.1 MD15G1236200.v1.1
prunus_persica Prupe.7G176300_v2.0.a1
pyrus_communis pycom02g09520 pycom15g21020
rosa_chinensis RchiOBHm_Chr2g0098181
rosa_laevigata RLG00000016769
rosa_multiflora Rmu_sc0009178.1_g000009 Rmu_sc0023861.1_g000003
rosa_roxburghii Rroxscaffold_2G00144220
rosa_rugosa Rorug02G0070800
rosa_samantha Rh2AG118100 Rh2BG121100 Rh2CG122700 Rh2DG122600
rosa_wichuraiana Rw2G009240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 194
AccB1I GGYRCC 2 cut(s) 86, 323
AciI CCGC 6 cut(s) 127, 200, 330, 333, 342, 671
AcsI RAATTY 1 cut(s) 298
AcuI CTGAAG 1 cut(s) 598
AcyI GRCGYC 2 cut(s) 191, 324
AdeI CACNNNGTG 2 cut(s) 434, 878
AfaI GTAC 2 cut(s) 548, 660
AfiI CCNNNNNNNGG 1 cut(s) 398
AgsI TTSAA 3 cut(s) 884, 925, 1001
AjiI CACGTC 1 cut(s) 905
AjnI CCWGG 1 cut(s) 943
AloI GAACNNNNNNTCC 2 cut(s) 632, 664
AluBI AGCT 7 cut(s) 149, 292, 442, 492, 527, 618, 692
AluI AGCT 7 cut(s) 149, 292, 442, 492, 527, 618, 692
Alw21I GWGCWC 1 cut(s) 733
Alw26I GTCTC 1 cut(s) 201
Alw44I GTGCAC 1 cut(s) 729
ApaLI GTGCAC 1 cut(s) 729
ApeKI GCWGC 4 cut(s) 439, 524, 527, 911
ApoI RAATTY 1 cut(s) 298
Asp700I GAANNNNTTC 1 cut(s) 1005
AspLEI GCGC 3 cut(s) 16, 326, 860
AsuC2I CCSGG 1 cut(s) 241
AsuHPI GGTGA 1 cut(s) 1045
AsuII TTCGAA 1 cut(s) 651
AsuNHI GCTAGC 1 cut(s) 488
BaeGI GKGCMC 2 cut(s) 91, 733
BanI GGYRCC 2 cut(s) 86, 323
BanII GRGCYC 3 cut(s) 115, 159, 246
BbsI GAAGAC 1 cut(s) 201
Bbv12I GWGCWC 1 cut(s) 733
BbvI GCAGC 4 cut(s) 451, 514, 536, 923
BccI CCATC 2 cut(s) 757, 802
BceAI ACGGC 2 cut(s) 187, 353
BciT130I CCWGG 1 cut(s) 945
BciVI GTATCC 1 cut(s) 748
BcnI CCSGG 1 cut(s) 241
BcoDI GTCTC 1 cut(s) 201
BfaI CTAG 2 cut(s) 489, 531
BfmI CTRYAG 1 cut(s) 837
BfoI RGCGCY 3 cut(s) 17, 327, 861
BfuI GTATCC 1 cut(s) 748
BglI GCCNNNNNGGC 1 cut(s) 321
BglII AGATCT 1 cut(s) 53
BisI GCNGC 7 cut(s) 128, 331, 343, 440, 525, 528, 912
BlpI GCTNAGC 1 cut(s) 145
BlsI GCNGC 7 cut(s) 129, 332, 344, 441, 526, 529, 913
Bme1390I CCNGG 2 cut(s) 241, 945
BmgBI CACGTC 1 cut(s) 905
BmiI GGNNCC 4 cut(s) 88, 156, 325, 384
BmrFI CCNGG 2 cut(s) 241, 945
BmsI GCATC 5 cut(s) 261, 295, 475, 835, 979
BmtI GCTAGC 1 cut(s) 492
BpiI GAAGAC 1 cut(s) 201
Bpu10I CCTNAGC 1 cut(s) 20
Bpu1102I GCTNAGC 1 cut(s) 145
Bpu14I TTCGAA 1 cut(s) 651
BpuEI CTTGAG 2 cut(s) 272, 727
BpuMI CCSGG 1 cut(s) 241
BsaBI GATNNNNATC 1 cut(s) 759
BsaHI GRCGYC 2 cut(s) 191, 324
BsaJI CCNNGG 3 cut(s) 9, 519, 943
BsaWI WCCGGW 1 cut(s) 181
BsaXI ACNNNNNCTCC 2 cut(s) 181, 211
Bsc4I CCNNNNNNNGG 1 cut(s) 398
Bse118I RCCGGY 2 cut(s) 320, 326
Bse8I GATNNNNATC 1 cut(s) 759
BseBI CCWGG 1 cut(s) 945
BseDI CCNNGG 3 cut(s) 9, 519, 943
BseGI GGATG 2 cut(s) 348, 1084
BseJI GATNNNNATC 1 cut(s) 759
BseLI CCNNNNNNNGG 1 cut(s) 398
BseMII CTCAG 3 cut(s) 11, 159, 771
BseRI GAGGAG 1 cut(s) 34
BseSI GKGCMC 2 cut(s) 91, 733
BseXI GCAGC 4 cut(s) 451, 514, 536, 923
BsgI GTGCAG 1 cut(s) 458
BshNI GGYRCC 2 cut(s) 86, 323
BsiHKAI GWGCWC 1 cut(s) 733
BsiSI CCGG 4 cut(s) 182, 240, 321, 327
BslFI GGGAC 1 cut(s) 520
BslI CCNNNNNNNGG 1 cut(s) 398
BsmAI GTCTC 1 cut(s) 201
BsmBI CGTCTC 1 cut(s) 201
BsmFI GGGAC 1 cut(s) 520
BsmI GAATGC 1 cut(s) 408
Bsp119I TTCGAA 1 cut(s) 651
Bsp1286I GDGCHC 5 cut(s) 91, 115, 159, 246, 733
Bsp1407I TGTACA 1 cut(s) 546
Bsp143I GATC 2 cut(s) 53, 760
Bsp1720I GCTNAGC 1 cut(s) 145
Bsp19I CCATGG 1 cut(s) 519
BspACI CCGC 6 cut(s) 127, 200, 330, 333, 342, 671
BspCNI CTCAG 3 cut(s) 12, 158, 772
BspLI GGNNCC 4 cut(s) 88, 156, 325, 384
BspOI GCTAGC 1 cut(s) 492
BspT104I TTCGAA 1 cut(s) 651
BspT107I GGYRCC 2 cut(s) 86, 323
BsrFI RCCGGY 2 cut(s) 320, 326
BsrGI TGTACA 1 cut(s) 546
BssAI RCCGGY 2 cut(s) 320, 326
BssECI CCNNGG 3 cut(s) 9, 519, 943
BssMI GATC 2 cut(s) 53, 760
BssNI GRCGYC 2 cut(s) 191, 324
BssT1I CCWWGG 2 cut(s) 9, 519
Bst2UI CCWGG 1 cut(s) 945
Bst4CI ACNGT 3 cut(s) 124, 359, 564
Bst6I CTCTTC 2 cut(s) 63, 393
BstACI GRCGYC 2 cut(s) 191, 324
BstAUI TGTACA 1 cut(s) 546
BstBI TTCGAA 1 cut(s) 651
BstC8I GCNNGC 3 cut(s) 328, 408, 490
BstDEI CTNAG 4 cut(s) 20, 145, 554, 780
BstDSI CCRYGG 1 cut(s) 519
BstF5I GGATG 2 cut(s) 348, 1084
BstH2I RGCGCY 3 cut(s) 17, 327, 861
BstHHI GCGC 3 cut(s) 16, 326, 860
BstKTI GATC 2 cut(s) 56, 763
BstMAI GTCTC 1 cut(s) 201
BstMBI GATC 2 cut(s) 53, 760
BstMWI GCNNNNNNNGC 6 cut(s) 11, 321, 330, 339, 533, 842
BstNI CCWGG 1 cut(s) 945
BstNSI RCATGY 1 cut(s) 984
BstSCI CCNGG 2 cut(s) 239, 943
BstSFI CTRYAG 1 cut(s) 837
BstSLI GKGCMC 2 cut(s) 91, 733
BstV1I GCAGC 4 cut(s) 451, 514, 536, 923
BstV2I GAAGAC 1 cut(s) 201
BstX2I RGATCY 1 cut(s) 53
BstYI RGATCY 1 cut(s) 53
BsuI GTATCC 1 cut(s) 748
BtgI CCRYGG 1 cut(s) 519
BtrI CACGTC 1 cut(s) 905
BtsCI GGATG 2 cut(s) 348, 1084
BtsIMutI CAGTG 1 cut(s) 364
Cac8I GCNNGC 3 cut(s) 328, 408, 490
CfoI GCGC 3 cut(s) 16, 326, 860
Cfr10I RCCGGY 2 cut(s) 320, 326
Csp6I GTAC 2 cut(s) 547, 659
CviAII CATG 3 cut(s) 520, 981, 1062
CviQI GTAC 2 cut(s) 547, 659
DdeI CTNAG 4 cut(s) 20, 145, 554, 780
DinI GGCGCC 1 cut(s) 325
DpnI GATC 2 cut(s) 55, 762
DpnII GATC 2 cut(s) 53, 760
DraI TTTAAA 1 cut(s) 636
DraIII CACNNNGTG 2 cut(s) 434, 878
Eam1104I CTCTTC 2 cut(s) 63, 393
EarI CTCTTC 2 cut(s) 63, 393
EciI GGCGGA 2 cut(s) 189, 348
Eco130I CCWWGG 2 cut(s) 9, 519
Eco24I GRGCYC 3 cut(s) 115, 159, 246
Eco57I CTGAAG 1 cut(s) 598
EcoRI GAATTC 1 cut(s) 298
EcoRII CCWGG 1 cut(s) 943
EcoT14I CCWWGG 2 cut(s) 9, 519
EcoT38I GRGCYC 3 cut(s) 115, 159, 246
EgeI GGCGCC 1 cut(s) 325
EheI GGCGCC 1 cut(s) 325
ErhI CCWWGG 2 cut(s) 9, 519
Esp3I CGTCTC 1 cut(s) 201
FaeI CATG 3 cut(s) 523, 984, 1065
FaqI GGGAC 1 cut(s) 520
FatI CATG 3 cut(s) 519, 980, 1061
FauI CCCGC 1 cut(s) 664
FauNDI CATATG 1 cut(s) 412
Fnu4HI GCNGC 7 cut(s) 128, 331, 343, 440, 525, 528, 912
FokI GGATG 2 cut(s) 335, 1071
FriOI GRGCYC 3 cut(s) 115, 159, 246
Fsp4HI GCNGC 7 cut(s) 128, 331, 343, 440, 525, 528, 912
FspBI CTAG 2 cut(s) 489, 531
GlaI GCGC 3 cut(s) 15, 325, 859
GluI GCNGC 7 cut(s) 128, 331, 343, 440, 525, 528, 912
HaeII RGCGCY 3 cut(s) 17, 327, 861
HapII CCGG 4 cut(s) 182, 240, 321, 327
HhaI GCGC 3 cut(s) 16, 326, 860
Hin1I GRCGYC 2 cut(s) 191, 324
Hin1II CATG 3 cut(s) 523, 984, 1065
Hin6I GCGC 3 cut(s) 14, 324, 858
HinP1I GCGC 3 cut(s) 14, 324, 858
HincII GTYRAC 1 cut(s) 447
HindII GTYRAC 1 cut(s) 447
HinfI GANTC 4 cut(s) 81, 173, 709, 1052
HpaII CCGG 4 cut(s) 182, 240, 321, 327
HphI GGTGA 1 cut(s) 1045
Hpy166II GTNNAC 4 cut(s) 447, 461, 731, 872
Hpy188I TCNGA 8 cut(s) 117, 154, 178, 248, 304, 484, 714, 754
Hpy188III TCNNGA 5 cut(s) 19, 263, 295, 570, 1056
Hpy8I GTNNAC 4 cut(s) 447, 461, 731, 872
Hpy99I CGWCG 4 cut(s) 193, 196, 208, 374
HpyAV CCTTC 6 cut(s) 45, 292, 319, 622, 910, 995
HpyCH4III ACNGT 3 cut(s) 124, 359, 564
HpyCH4IV ACGT 2 cut(s) 191, 904
HpyCH4V TGCA 5 cut(s) 410, 439, 452, 731, 821
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 321, 330, 339, 533, 842
HpyF3I CTNAG 4 cut(s) 20, 145, 554, 780
HpySE526I ACGT 2 cut(s) 191, 904
Hsp92I GRCGYC 2 cut(s) 191, 324
Hsp92II CATG 3 cut(s) 523, 984, 1065
HspAI GCGC 3 cut(s) 14, 324, 858
KasI GGCGCC 1 cut(s) 323
KroI GCCGGC 1 cut(s) 326
KroNI GCCGGC 1 cut(s) 328
Kzo9I GATC 2 cut(s) 53, 760
LmnI GCTCC 2 cut(s) 21, 154
Lsp1109I GCAGC 4 cut(s) 451, 514, 536, 923
LweI GCATC 5 cut(s) 261, 295, 475, 835, 979
MaeI CTAG 2 cut(s) 489, 531
MaeII ACGT 2 cut(s) 191, 904
MaeIII GTNAC 2 cut(s) 215, 706
MalI GATC 2 cut(s) 55, 762
MboI GATC 2 cut(s) 53, 760
MboII GAAGA 4 cut(s) 50, 201, 380, 755
MflI RGATCY 1 cut(s) 53
MhlI GDGCHC 5 cut(s) 91, 115, 159, 246, 733
MluCI AATT 3 cut(s) 298, 471, 920
Mly113I GGCGCC 1 cut(s) 324
MlyI GAGTC 1 cut(s) 703
MreI CGCCGGCG 1 cut(s) 326
MroNI GCCGGC 1 cut(s) 326
MroXI GAANNNNTTC 1 cut(s) 1005
MseI TTAA 1 cut(s) 635
MslI CAYNNNNRTG 1 cut(s) 831
MspA1I CMGCKG 2 cut(s) 442, 527
MspI CCGG 4 cut(s) 182, 240, 321, 327
MspR9I CCNGG 2 cut(s) 241, 945
Mva1269I GAATGC 1 cut(s) 408
MvaI CCWGG 1 cut(s) 945
MwoI GCNNNNNNNGC 6 cut(s) 11, 321, 330, 339, 533, 842
NaeI GCCGGC 1 cut(s) 328
NarI GGCGCC 1 cut(s) 324
NciI CCSGG 1 cut(s) 241
NcoI CCATGG 1 cut(s) 519
NdeI CATATG 1 cut(s) 412
NdeII GATC 2 cut(s) 53, 760
NgoMIV GCCGGC 1 cut(s) 326
NheI GCTAGC 1 cut(s) 488
NlaIII CATG 3 cut(s) 523, 984, 1065
NlaIV GGNNCC 4 cut(s) 88, 156, 325, 384
NmuCI GTSAC 2 cut(s) 215, 706
NspI RCATGY 1 cut(s) 984
NspV TTCGAA 1 cut(s) 651
PcsI WCGNNNNNNNCGW 4 cut(s) 174, 200, 261, 575
PctI GAATGC 1 cut(s) 408
PdiI GCCGGC 1 cut(s) 328
PdmI GAANNNNTTC 1 cut(s) 1005
PfeI GAWTC 3 cut(s) 81, 173, 1052
PflFI GACNNNGTC 1 cut(s) 193
PkrI GCNGC 7 cut(s) 129, 332, 344, 441, 526, 529, 913
PleI GAGTC 1 cut(s) 703
PluTI GGCGCC 1 cut(s) 327
PpsI GAGTC 1 cut(s) 703
Psp6I CCWGG 1 cut(s) 943
PspGI CCWGG 1 cut(s) 943
PspN4I GGNNCC 4 cut(s) 88, 156, 325, 384
PsuI RGATCY 1 cut(s) 53
PsyI GACNNNGTC 1 cut(s) 193
PvuII CAGCTG 2 cut(s) 442, 527
RsaI GTAC 2 cut(s) 548, 660
RsaNI GTAC 2 cut(s) 547, 659
RseI CAYNNNNRTG 1 cut(s) 831
SaqAI TTAA 1 cut(s) 635
SatI GCNGC 7 cut(s) 128, 331, 343, 440, 525, 528, 912
Sau3AI GATC 2 cut(s) 53, 760
SchI GAGTC 1 cut(s) 703
ScrFI CCNGG 2 cut(s) 241, 945
SduI GDGCHC 5 cut(s) 91, 115, 159, 246, 733
SfaNI GCATC 5 cut(s) 261, 295, 475, 835, 979
SfcI CTRYAG 1 cut(s) 837
SfoI GGCGCC 1 cut(s) 325
SfuI TTCGAA 1 cut(s) 651
SgrAI CRCCGGYG 2 cut(s) 320, 326
SmiMI CAYNNNNRTG 1 cut(s) 831
SmlI CTYRAG 2 cut(s) 287, 742
SmoI CTYRAG 2 cut(s) 287, 742
Sse9I AATT 3 cut(s) 298, 471, 920
SsiI CCGC 6 cut(s) 127, 200, 330, 333, 342, 671
SspDI GGCGCC 1 cut(s) 323
SspMI CTAG 2 cut(s) 489, 531
StyD4I CCNGG 2 cut(s) 239, 943
StyI CCWWGG 2 cut(s) 9, 519
TaaI ACNGT 3 cut(s) 124, 359, 564
TaiI ACGT 2 cut(s) 194, 907
TaqI TCGA 3 cut(s) 212, 264, 651
TasI AATT 3 cut(s) 298, 471, 920
TatI WGTACW 2 cut(s) 546, 658
TauI GCSGC 3 cut(s) 130, 333, 345
TfiI GAWTC 3 cut(s) 81, 173, 1052
Tru1I TTAA 1 cut(s) 635
Tru9I TTAA 1 cut(s) 635
TscAI CASTG 1 cut(s) 364
TseFI GTSAC 2 cut(s) 215, 706
TseI GCWGC 4 cut(s) 439, 524, 527, 911
Tsp45I GTSAC 2 cut(s) 215, 706
TspDTI ATGAA 2 cut(s) 616, 1073
TspGWI ACGGA 2 cut(s) 381, 792
TspRI CASTG 1 cut(s) 364
Tth111I GACNNNGTC 1 cut(s) 193
VneI GTGCAC 1 cut(s) 729
XapI RAATTY 1 cut(s) 298
XceI RCATGY 1 cut(s) 984
XmnI GAANNNNTTC 1 cut(s) 1005
XspI CTAG 2 cut(s) 489, 531
ZraI GACGTC 1 cut(s) 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.