Rroxscaffold_2G00155300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0009923
Physical Location & Seq
Forward (+)
91697624 .. 91700453
2830 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00155300.1

Sequence Viewer

Length: 780 bp
ATGAAGAAGATGAAAGGGGTTGTTGCTGTGGAGCCTTCTTCTTATGCTCTGTATCAGGATTCAAAGGCCAGGCTTAAGCATCAGAGTCTTATGCAAGACTATGAGGAGCTGCAGAAGGATGCAGAGGCCATGAAGAACAGATTGGAGATGATGAAACAGAGAAAGTCTATCCTTACGGCTGAAGTCAGATTTTTGAGGAGAAGATACAAGTACTTGATTGGGAAGCAGTCCATGAACCCCAAACTGAAGGAAGACCTTGTGCAAACACACAATTTGAAAATCCAGCGAACCGATGTTTCGAAGGGAAAGAATTACAGTAAGAAGGAATCTGCTTTGAGACATCCTGCTCCTGCAATGGACTTGAACCAAAGGGTACGGATTAAAAATGCAATGGAAGTAGCCTTGCAAAAATCTACTCCAAGTTCTGACTTGAACCATAAGGCAAGGACTGTCAGCCGGAAGGAAACTACTCTGCATAACTCAAATCCAGTTATTGACTTAAATCAGAAGGAAAGGATTCAGAGTGGGAAGGAATCAACCAAGCGAAAAAAAACTCCAGTTTTTGACTTGAACCAAGTTTCGCTAGAGGAAGAAGAGGAAACTCAGGCTGATTGTGAGCCATTGAGGACAGACGAGCCAAACAAAAGTATACTTAGAGGGGGTATTGATGAGCAGCACAATGATATGAAGTTGCTGGTTTGCAGGACTATCGGGAATGGGTCGAACCGATCAGGGAAGAGGAAGATTACTTGGCAAGATCCGGTTGCCTTAAGGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

259

Amino Acids

29.9

Weight (kDa)

9.84

Isoelectric Point (pI)

54.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 649
AclWI GGATC 1 cut(s) 752
AcuI CTGAAG 2 cut(s) 201, 266
AfaI GTAC 2 cut(s) 212, 375
AflII CTTAAG 2 cut(s) 74, 769
AgsI TTSAA 5 cut(s) 63, 277, 364, 433, 571
AjnI CCWGG 1 cut(s) 68
AjuI GAANNNNNNNTTGG 2 cut(s) 125, 157
AluBI AGCT 1 cut(s) 109
AluI AGCT 1 cut(s) 109
Alw26I GTCTC 1 cut(s) 331
AlwI GGATC 1 cut(s) 752
AoxI GGCC 2 cut(s) 66, 126
ApeKI GCWGC 2 cut(s) 109, 673
Asp700I GAANNNNTTC 1 cut(s) 516
AsuII TTCGAA 1 cut(s) 299
BbsI GAAGAC 1 cut(s) 258
BbvI GCAGC 2 cut(s) 96, 685
BceAI ACGGC 1 cut(s) 192
BcgI CGANNNNNNTGC 2 cut(s) 691, 725
BciT130I CCWGG 1 cut(s) 70
BcoDI GTCTC 1 cut(s) 331
BfaI CTAG 1 cut(s) 584
BfmI CTRYAG 1 cut(s) 110
BfrI CTTAAG 2 cut(s) 74, 769
BisI GCNGC 2 cut(s) 110, 674
BlsI GCNGC 2 cut(s) 111, 675
BmcAI AGTACT 1 cut(s) 212
Bme1390I CCNGG 1 cut(s) 70
BmiI GGNNCC 1 cut(s) 33
BmrFI CCNGG 1 cut(s) 70
BmsI GCATC 2 cut(s) 88, 109
BpiI GAAGAC 1 cut(s) 258
BpmI CTGGAG 1 cut(s) 540
Bpu14I TTCGAA 1 cut(s) 299
BsaWI WCCGGW 1 cut(s) 760
Bse1I ACTGG 2 cut(s) 488, 557
Bse3DI GCAATG 2 cut(s) 360, 396
BseBI CCWGG 1 cut(s) 70
BseGI GGATG 2 cut(s) 124, 340
BseMI GCAATG 2 cut(s) 360, 396
BseMII CTCAG 1 cut(s) 617
BseNI ACTGG 2 cut(s) 488, 557
BseRI GAGGAG 2 cut(s) 119, 211
BseXI GCAGC 2 cut(s) 96, 685
BshFI GGCC 2 cut(s) 68, 128
BsiSI CCGG 2 cut(s) 457, 761
BsmAI GTCTC 1 cut(s) 331
BsnI GGCC 2 cut(s) 68, 128
Bsp119I TTCGAA 1 cut(s) 299
Bsp143I GATC 2 cut(s) 728, 757
BspANI GGCC 2 cut(s) 68, 128
BspCNI CTCAG 1 cut(s) 616
BspLI GGNNCC 1 cut(s) 33
BspMAI CTGCAG 1 cut(s) 114
BspPI GGATC 1 cut(s) 752
BspT104I TTCGAA 1 cut(s) 299
BspTI CTTAAG 2 cut(s) 74, 769
BsrDI GCAATG 2 cut(s) 360, 396
BsrI ACTGG 2 cut(s) 488, 557
BssMI GATC 2 cut(s) 728, 757
BssNAI GTATAC 1 cut(s) 650
Bst1107I GTATAC 1 cut(s) 650
Bst2UI CCWGG 1 cut(s) 70
Bst4CI ACNGT 2 cut(s) 317, 451
Bst6I CTCTTC 2 cut(s) 588, 731
BstAFI CTTAAG 2 cut(s) 74, 769
BstBI TTCGAA 1 cut(s) 299
BstDEI CTNAG 2 cut(s) 603, 653
BstF5I GGATG 2 cut(s) 124, 340
BstKTI GATC 2 cut(s) 731, 760
BstMAI GTCTC 1 cut(s) 331
BstMBI GATC 2 cut(s) 728, 757
BstNI CCWGG 1 cut(s) 70
BstSCI CCNGG 1 cut(s) 68
BstSFI CTRYAG 1 cut(s) 110
BstV1I GCAGC 2 cut(s) 96, 685
BstV2I GAAGAC 1 cut(s) 258
BstX2I RGATCY 1 cut(s) 757
BstYI RGATCY 1 cut(s) 757
BstZ17I GTATAC 1 cut(s) 650
BsuRI GGCC 2 cut(s) 68, 128
BtsCI GGATG 2 cut(s) 124, 340
Csp6I GTAC 2 cut(s) 211, 374
CviAII CATG 2 cut(s) 130, 232
CviQI GTAC 2 cut(s) 211, 374
DdeI CTNAG 2 cut(s) 603, 653
DpnI GATC 2 cut(s) 730, 759
DpnII GATC 2 cut(s) 728, 757
Eam1104I CTCTTC 2 cut(s) 588, 731
EarI CTCTTC 2 cut(s) 588, 731
Eco57I CTGAAG 2 cut(s) 201, 266
EcoRII CCWGG 1 cut(s) 68
FaeI CATG 2 cut(s) 133, 235
FaiI YATR 9 cut(s) 45, 92, 102, 131, 233, 438, 477, 650, 686
FatI CATG 2 cut(s) 129, 231
FblI GTMKAC 1 cut(s) 649
Fnu4HI GCNGC 2 cut(s) 110, 674
FokI GGATG 2 cut(s) 131, 327
Fsp4HI GCNGC 2 cut(s) 110, 674
FspBI CTAG 1 cut(s) 584
GluI GCNGC 2 cut(s) 110, 674
GsuI CTGGAG 1 cut(s) 540
HaeIII GGCC 2 cut(s) 68, 128
HapII CCGG 2 cut(s) 457, 761
Hin1II CATG 2 cut(s) 133, 235
HinfI GANTC 5 cut(s) 59, 85, 326, 517, 533
HpaII CCGG 2 cut(s) 457, 761
Hpy166II GTNNAC 1 cut(s) 650
Hpy188I TCNGA 5 cut(s) 84, 188, 427, 507, 522
Hpy188III TCNNGA 2 cut(s) 56, 712
Hpy8I GTNNAC 1 cut(s) 650
HpyAV CCTTC 8 cut(s) 45, 109, 241, 295, 316, 454, 502, 523
HpyCH4III ACNGT 2 cut(s) 317, 451
HpyCH4V TGCA 9 cut(s) 94, 112, 122, 262, 353, 389, 406, 475, 702
HpyF3I CTNAG 2 cut(s) 603, 653
Hsp92II CATG 2 cut(s) 133, 235
Kzo9I GATC 2 cut(s) 728, 757
LmnI GCTCC 3 cut(s) 31, 106, 352
Lsp1109I GCAGC 2 cut(s) 96, 685
LweI GCATC 2 cut(s) 88, 109
MaeI CTAG 1 cut(s) 584
MalI GATC 2 cut(s) 730, 759
MboI GATC 2 cut(s) 728, 757
MflI RGATCY 1 cut(s) 757
MluCI AATT 2 cut(s) 271, 310
MlyI GAGTC 1 cut(s) 94
MnlI CCTC 8 cut(s) 97, 118, 189, 580, 589, 618, 650, 732
MroXI GAANNNNTTC 1 cut(s) 516
MseI TTAA 4 cut(s) 75, 381, 500, 770
MspCI CTTAAG 2 cut(s) 74, 769
MspI CCGG 2 cut(s) 457, 761
MspR9I CCNGG 1 cut(s) 70
MvaI CCWGG 1 cut(s) 70
NdeII GATC 2 cut(s) 728, 757
NlaIII CATG 2 cut(s) 133, 235
NlaIV GGNNCC 1 cut(s) 33
NspV TTCGAA 1 cut(s) 299
PdmI GAANNNNTTC 1 cut(s) 516
PfeI GAWTC 4 cut(s) 59, 326, 517, 533
PkrI GCNGC 2 cut(s) 111, 675
PleI GAGTC 1 cut(s) 93
PpsI GAGTC 1 cut(s) 93
Psp6I CCWGG 1 cut(s) 68
PspGI CCWGG 1 cut(s) 68
PspN4I GGNNCC 1 cut(s) 33
PstI CTGCAG 1 cut(s) 114
PsuI RGATCY 1 cut(s) 757
RsaI GTAC 2 cut(s) 212, 375
RsaNI GTAC 2 cut(s) 211, 374
SaqAI TTAA 4 cut(s) 75, 381, 500, 770
SatI GCNGC 2 cut(s) 110, 674
Sau3AI GATC 2 cut(s) 728, 757
ScaI AGTACT 1 cut(s) 212
SchI GAGTC 1 cut(s) 94
ScrFI CCNGG 1 cut(s) 70
SetI ASST 2 cut(s) 111, 258
SfaNI GCATC 2 cut(s) 88, 109
SfcI CTRYAG 1 cut(s) 110
SfuI TTCGAA 1 cut(s) 299
SmlI CTYRAG 2 cut(s) 74, 769
SmoI CTYRAG 2 cut(s) 74, 769
Sse9I AATT 2 cut(s) 271, 310
SspMI CTAG 1 cut(s) 584
StyD4I CCNGG 1 cut(s) 68
TaaI ACNGT 2 cut(s) 317, 451
TaqI TCGA 2 cut(s) 299, 722
TasI AATT 2 cut(s) 271, 310
TatI WGTACW 1 cut(s) 210
TfiI GAWTC 4 cut(s) 59, 326, 517, 533
Tru1I TTAA 4 cut(s) 75, 381, 500, 770
Tru9I TTAA 4 cut(s) 75, 381, 500, 770
TseI GCWGC 2 cut(s) 109, 673
TspDTI ATGAA 6 cut(s) 17, 26, 146, 167, 248, 701
TspGWI ACGGA 1 cut(s) 391
Vha464I CTTAAG 2 cut(s) 74, 769
XmiI GTMKAC 1 cut(s) 649
XmnI GAANNNNTTC 1 cut(s) 516
XspI CTAG 1 cut(s) 584
ZrmI AGTACT 1 cut(s) 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.