Rroxscaffold_3G00227420

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0008165
Physical Location & Seq
Forward (+)
11518574 .. 11524857
6284 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00227420.1

Sequence Viewer

Length: 414 bp
ATGAAACATAAACAAAGGTCTAACGAAGCTAGAAGACCAAACGAGCAGCAGAGGAACTCCGAAGCGGCCGAGATTAGGTCCGAAGCGAAATGGTCCGACTTGCGAAGAAGACCAAATGAGCAGCACGGGGACTCCGAAGCGGCTGAGATTAGGTCTCTCTCTCGATCCGGGTCTAAGATTGAAAGCATTGCAGGCGGCATTCGAACCACAGAGGTAGTCATGCTTGAAGCTTCGACATGTCTTCCGGATTGCTCTAGTTTATTCCATCCTTTGTGGCGTCCGGATTTTTTTCATTCATGGTGGGTTGTGAGCTTCGACATTCATTGTCTTCACTACTCAAGCACCGCCGCAGCCCACTTTGTGTTTTCTGCAGCCTTTTGTGATCGCAGTGAGCCAAGTAGAACACGCCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

137

Amino Acids

15.76

Weight (kDa)

8.47

Isoelectric Point (pI)

88.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 2 cut(s) 244, 280
AciI CCGC 6 cut(s) 65, 140, 195, 345, 348, 409
AclWI GGATC 1 cut(s) 159
AcoI YGGCCR 1 cut(s) 66
AcyI GRCGYC 1 cut(s) 277
AdeI CACNNNGTG 1 cut(s) 361
AfiI CCNNNNNNNGG 1 cut(s) 75
AflIII ACRYGT 1 cut(s) 236
AgsI TTSAA 2 cut(s) 182, 227
AluBI AGCT 3 cut(s) 29, 230, 312
AluI AGCT 3 cut(s) 29, 230, 312
Alw26I GTCTC 1 cut(s) 159
AlwI GGATC 1 cut(s) 159
Aor13HI TCCGGA 2 cut(s) 244, 280
AoxI GGCC 1 cut(s) 66
ApeKI GCWGC 4 cut(s) 46, 121, 350, 371
AspS9I GGNCC 2 cut(s) 78, 93
AsuC2I CCSGG 1 cut(s) 169
AsuII TTCGAA 1 cut(s) 202
AvaII GGWCC 2 cut(s) 78, 93
BbsI GAAGAC 4 cut(s) 40, 115, 233, 320
BbvI GCAGC 4 cut(s) 58, 133, 362, 383
BccI CCATC 1 cut(s) 273
BcnI CCSGG 1 cut(s) 169
BcoDI GTCTC 1 cut(s) 159
BfaI CTAG 2 cut(s) 30, 255
BfmI CTRYAG 1 cut(s) 369
BisI GCNGC 9 cut(s) 47, 66, 122, 141, 196, 348, 351, 372, 409
BlsI GCNGC 9 cut(s) 48, 67, 123, 142, 197, 349, 352, 373, 410
Bme1390I CCNGG 1 cut(s) 169
Bme18I GGWCC 2 cut(s) 78, 93
BmgT120I GGNCC 2 cut(s) 78, 93
BmrFI CCNGG 1 cut(s) 169
BpiI GAAGAC 4 cut(s) 40, 115, 233, 320
Bpu14I TTCGAA 1 cut(s) 202
BpuEI CTTGAG 1 cut(s) 322
BpuMI CCSGG 1 cut(s) 169
BsaHI GRCGYC 1 cut(s) 277
BsaI GGTCTC 1 cut(s) 159
BsaWI WCCGGW 2 cut(s) 244, 280
BsaXI ACNNNNNCTCC 2 cut(s) 116, 146
Bsc4I CCNNNNNNNGG 1 cut(s) 75
Bse3DI GCAATG 1 cut(s) 186
BseAI TCCGGA 2 cut(s) 244, 280
BseGI GGATG 1 cut(s) 265
BseLI CCNNNNNNNGG 1 cut(s) 75
BseMI GCAATG 1 cut(s) 186
BseMII CTCAG 1 cut(s) 135
BseX3I CGGCCG 1 cut(s) 66
BseXI GCAGC 4 cut(s) 58, 133, 362, 383
Bsh1285I CGRYCG 1 cut(s) 69
BshFI GGCC 1 cut(s) 68
BsiEI CGRYCG 1 cut(s) 69
BsiSI CCGG 3 cut(s) 168, 245, 281
BslFI GGGAC 1 cut(s) 143
BslI CCNNNNNNNGG 1 cut(s) 75
BsmAI GTCTC 1 cut(s) 159
BsmFI GGGAC 1 cut(s) 143
BsmI GAATGC 1 cut(s) 198
BsnI GGCC 1 cut(s) 68
Bso31I GGTCTC 1 cut(s) 159
Bsp119I TTCGAA 1 cut(s) 202
Bsp13I TCCGGA 2 cut(s) 244, 280
Bsp143I GATC 2 cut(s) 164, 382
BspACI CCGC 6 cut(s) 65, 140, 195, 345, 348, 409
BspANI GGCC 1 cut(s) 68
BspCNI CTCAG 1 cut(s) 136
BspEI TCCGGA 2 cut(s) 244, 280
BspMAI CTGCAG 1 cut(s) 373
BspPI GGATC 1 cut(s) 159
BspT104I TTCGAA 1 cut(s) 202
BspTNI GGTCTC 1 cut(s) 159
BsrDI GCAATG 1 cut(s) 186
BssMI GATC 2 cut(s) 164, 382
BssNI GRCGYC 1 cut(s) 277
BstACI GRCGYC 1 cut(s) 277
BstBI TTCGAA 1 cut(s) 202
BstC8I GCNNGC 1 cut(s) 193
BstDEI CTNAG 2 cut(s) 144, 174
BstF5I GGATG 1 cut(s) 265
BstKTI GATC 2 cut(s) 167, 385
BstMAI GTCTC 1 cut(s) 159
BstMBI GATC 2 cut(s) 164, 382
BstMCI CGRYCG 1 cut(s) 69
BstMWI GCNNNNNNNGC 1 cut(s) 192
BstNSI RCATGY 1 cut(s) 240
BstSCI CCNGG 1 cut(s) 167
BstSFI CTRYAG 1 cut(s) 369
BstV1I GCAGC 4 cut(s) 58, 133, 362, 383
BstV2I GAAGAC 4 cut(s) 40, 115, 233, 320
BstZI CGGCCG 1 cut(s) 66
BsuRI GGCC 1 cut(s) 68
BtsCI GGATG 1 cut(s) 265
BtsI GCAGTG 1 cut(s) 394
BtsIMutI CAGTG 1 cut(s) 394
Cac8I GCNNGC 1 cut(s) 193
Cfr13I GGNCC 2 cut(s) 78, 93
CseI GACGC 1 cut(s) 266
CviAII CATG 3 cut(s) 220, 237, 297
CviJI RGCY 8 cut(s) 29, 68, 143, 230, 312, 353, 374, 394
CviKI_1 RGCY 8 cut(s) 29, 68, 143, 230, 312, 353, 374, 394
DdeI CTNAG 2 cut(s) 144, 174
DpnI GATC 2 cut(s) 166, 384
DpnII GATC 2 cut(s) 164, 382
DraIII CACNNNGTG 1 cut(s) 361
EaeI YGGCCR 1 cut(s) 66
EagI CGGCCG 1 cut(s) 66
EclXI CGGCCG 1 cut(s) 66
Eco31I GGTCTC 1 cut(s) 159
Eco47I GGWCC 2 cut(s) 78, 93
Eco52I CGGCCG 1 cut(s) 66
FaeI CATG 3 cut(s) 223, 240, 300
FaiI YATR 4 cut(s) 9, 221, 238, 298
FaqI GGGAC 1 cut(s) 143
FatI CATG 3 cut(s) 219, 236, 296
Fnu4HI GCNGC 9 cut(s) 47, 66, 122, 141, 196, 348, 351, 372, 409
FokI GGATG 1 cut(s) 252
Fsp4HI GCNGC 9 cut(s) 47, 66, 122, 141, 196, 348, 351, 372, 409
FspBI CTAG 2 cut(s) 30, 255
GluI GCNGC 9 cut(s) 47, 66, 122, 141, 196, 348, 351, 372, 409
HaeIII GGCC 1 cut(s) 68
HapII CCGG 3 cut(s) 168, 245, 281
HgaI GACGC 1 cut(s) 266
Hin1I GRCGYC 1 cut(s) 277
Hin1II CATG 3 cut(s) 223, 240, 300
HindIII AAGCTT 1 cut(s) 228
HinfI GANTC 1 cut(s) 131
HpaII CCGG 3 cut(s) 168, 245, 281
Hpy188I TCNGA 4 cut(s) 61, 82, 97, 136
Hpy188III TCNNGA 3 cut(s) 162, 245, 281
HpyCH4V TGCA 2 cut(s) 191, 371
HpyF10VI GCNNNNNNNGC 1 cut(s) 192
HpyF3I CTNAG 2 cut(s) 144, 174
Hsp92I GRCGYC 1 cut(s) 277
Hsp92II CATG 3 cut(s) 223, 240, 300
Kpn2I TCCGGA 2 cut(s) 244, 280
Kzo9I GATC 2 cut(s) 164, 382
LpnPI CCDG 4 cut(s) 177, 181, 258, 294
Lsp1109I GCAGC 4 cut(s) 58, 133, 362, 383
MaeI CTAG 2 cut(s) 30, 255
MalI GATC 2 cut(s) 166, 384
MboI GATC 2 cut(s) 164, 382
MboII GAAGA 5 cut(s) 45, 117, 120, 233, 320
MlyI GAGTC 1 cut(s) 125
MmeI TCCRAC 1 cut(s) 120
MnlI CCTC 2 cut(s) 45, 205
MroI TCCGGA 2 cut(s) 244, 280
MspA1I CMGCKG 1 cut(s) 411
MspI CCGG 3 cut(s) 168, 245, 281
MspR9I CCNGG 1 cut(s) 169
Mva1269I GAATGC 1 cut(s) 198
MwoI GCNNNNNNNGC 1 cut(s) 192
NciI CCSGG 1 cut(s) 169
NdeII GATC 2 cut(s) 164, 382
NlaIII CATG 3 cut(s) 223, 240, 300
NmeAIII GCCGAG 1 cut(s) 94
NspI RCATGY 1 cut(s) 240
NspV TTCGAA 1 cut(s) 202
PciI ACATGT 1 cut(s) 236
PcsI WCGNNNNNNNCGW 1 cut(s) 132
PctI GAATGC 1 cut(s) 198
PkrI GCNGC 9 cut(s) 48, 67, 123, 142, 197, 349, 352, 373, 410
PleI GAGTC 1 cut(s) 125
PpsI GAGTC 1 cut(s) 125
PscI ACATGT 1 cut(s) 236
PspPI GGNCC 2 cut(s) 78, 93
PstI CTGCAG 1 cut(s) 373
SatI GCNGC 9 cut(s) 47, 66, 122, 141, 196, 348, 351, 372, 409
Sau3AI GATC 2 cut(s) 164, 382
Sau96I GGNCC 2 cut(s) 78, 93
SchI GAGTC 1 cut(s) 125
ScrFI CCNGG 1 cut(s) 169
SetI ASST 7 cut(s) 20, 31, 80, 155, 216, 232, 314
SfcI CTRYAG 1 cut(s) 369
SfuI TTCGAA 1 cut(s) 202
SinI GGWCC 2 cut(s) 78, 93
SmlI CTYRAG 1 cut(s) 337
SmoI CTYRAG 1 cut(s) 337
SsiI CCGC 6 cut(s) 65, 140, 195, 345, 348, 409
SspMI CTAG 2 cut(s) 30, 255
StyD4I CCNGG 1 cut(s) 167
TaqI TCGA 4 cut(s) 163, 202, 233, 315
TauI GCSGC 5 cut(s) 68, 143, 198, 350, 411
TscAI CASTG 1 cut(s) 394
TseI GCWGC 4 cut(s) 46, 121, 350, 371
TspDTI ATGAA 4 cut(s) 17, 281, 285, 311
TspRI CASTG 1 cut(s) 394
VpaK11BI GGWCC 2 cut(s) 78, 93
XceI RCATGY 1 cut(s) 240
XspI CTAG 2 cut(s) 30, 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.