Rroxscaffold_4G00284690

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0009063
Physical Location & Seq
Forward (+)
6272597 .. 6274405
1809 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00284690.1

Sequence Viewer

Length: 1200 bp
ATGATGTATACAAGGACTGCTAGTGGTTCAACTAATCTCGAAGAACGGATGGTAACACGCGCAGGTCGAAAGCTGTTTTATCCTGGTATGGGGAAACTCTGGGTTGAAGTTTGCTTGATATCTGCTCGAGGGCTCCGGCGTTCGTCGTCCCTATGGAAGCTTCAATGGTATGCTGTGGGGTGGACTAATCCCAACAACAAGTACTGCACCAAGATTGATGCATCTGGAAATGCAAATCCAGTATGGAAAACCAAGTTCGCTACCTTGGTTGAGGACGCAGAATCTAAACTGAATGATTTGGCTCTGCACATTGAAGTCTATAGTAGAGAGCCTATATTCCTCAGAGAAAGGCTACAGGGGACAGCTACTATTCTCTTGAAAGAGTTTCTGGCTAAGCATAACAAGAATTCTGAGAATTCAAGGCATGGAGTTGAAGAAGTTGGGAGCTACCAATTGCGAAAAAGAATTCCAACAAACCTCAAGGGTTTGTTGATGTTTCAATCCACATTTCTGAGGATATGGAAGGGAGAAGCTCTCATACACAGTTTCGTGTTTAATGGAAATATGATCCTTGAGAATCACCATAAATGTGATGAAATCAAGTTCTGCTTTGTAGGAAATGAGGGAGGACCTGGGGATCACAGCAATACTATCACCTTGGCTACCGGAGATGGGTCTGCACCAGCATTACTACCTTTTACTCCACAAAAGAGACCAGAACATCAATTCCAAATGAATTCCCCGTATGCAAATCCAATGCTTTCGACTACAAACTATTCGAAACCATCTGCAGTTGGAACAAGTTACACACCAGCAAGCGGACCAAGCTACACGCCAGCAAACGGACCAGGCTACCTGCCAGCAAGCGGACCAGGCTACCTGCCAGCAAGTGGACCAAGCTATCAGCCTTCTAGAACTCCTCCCCCGCCTCCACCACCTTCCAATGTTGGATATGTACCTACTTTTCTCCCCAGGACAGATCAAATGACTGACACCTATATCAATGTGCCCTCATCATCTGGAGCACCACCTCCTGGTCGTGGAACTAGGGCCGGAGGACCTGCTTTTGCAATGGGAATGGGTGCTGGAGCGCTGGCAGCCGGTGCCGTGATCTTCGGTGATGACTTTATGTCAGGATTTGATGTTCCTTCAGGCTTACAAGATGCTAGTGTTACCATATCAGCTGATCCTCCTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

399

Amino Acids

43.23

Weight (kDa)

9.11

Isoelectric Point (pI)

48.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 35 - 135 2.8e-08 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 4 cut(s) 53, 864, 888, 1069
AccB1I GGYRCC 1 cut(s) 1103
AccB7I CCANNNNNTGG 2 cut(s) 890, 1034
AccI GTMKAC 1 cut(s) 8
AccII CGCG 1 cut(s) 60
AciI CCGC 3 cut(s) 819, 867, 926
AclWI GGATC 3 cut(s) 562, 645, 1181
AcsI RAATTY 4 cut(s) 406, 415, 465, 736
AcuI CTGAAG 1 cut(s) 1134
AfaI GTAC 2 cut(s) 203, 957
AfeI AGCGCT 1 cut(s) 1092
AfiI CCNNNNNNNGG 8 cut(s) 89, 672, 818, 842, 866, 890, 1034, 1040
AgsI TTSAA 8 cut(s) 30, 107, 164, 314, 379, 420, 434, 500
AhdI GACNNNNNGTC 1 cut(s) 1129
AjnI CCWGG 6 cut(s) 82, 631, 847, 871, 971, 1033
AloI GAACNNNNNNTCC 4 cut(s) 711, 743, 1128, 1160
AluBI AGCT 8 cut(s) 73, 160, 365, 447, 533, 828, 900, 1184
AluI AGCT 8 cut(s) 73, 160, 365, 447, 533, 828, 900, 1184
Alw21I GWGCWC 1 cut(s) 1027
Alw26I GTCTC 1 cut(s) 706
AlwI GGATC 3 cut(s) 562, 645, 1181
Ama87I CYCGRG 1 cut(s) 126
Aor51HI AGCGCT 1 cut(s) 1092
AoxI GGCC 1 cut(s) 1050
ApeKI GCWGC 1 cut(s) 1097
ApoI RAATTY 4 cut(s) 406, 415, 465, 736
AspLEI GCGC 2 cut(s) 62, 1093
AspS9I GGNCC 7 cut(s) 629, 821, 845, 869, 893, 1050, 1058
AsuHPI GGTGA 3 cut(s) 572, 646, 1130
AsuII TTCGAA 1 cut(s) 779
AvaI CYCGRG 1 cut(s) 126
AvaII GGWCC 6 cut(s) 629, 821, 845, 869, 893, 1058
BaeGI GKGCMC 1 cut(s) 1011
BanI GGYRCC 1 cut(s) 1103
BanII GRGCYC 1 cut(s) 135
BarI GAAGNNNNNNTAC 2 cut(s) 522, 554
Bbv12I GWGCWC 1 cut(s) 1027
BbvI GCAGC 1 cut(s) 1109
BccI CCATC 3 cut(s) 43, 665, 793
BceAI ACGGC 1 cut(s) 1091
BciT130I CCWGG 6 cut(s) 84, 633, 849, 873, 973, 1035
BcoDI GTCTC 1 cut(s) 706
BfaI CTAG 4 cut(s) 21, 912, 1047, 1167
BfmI CTRYAG 3 cut(s) 319, 353, 789
BfoI RGCGCY 1 cut(s) 1094
BfuAI ACCTGC 4 cut(s) 53, 864, 888, 1069
BisI GCNGC 1 cut(s) 1098
BlpI GCTNAGC 1 cut(s) 393
BlsI GCNGC 1 cut(s) 1099
BmcAI AGTACT 1 cut(s) 203
Bme1390I CCNGG 6 cut(s) 84, 633, 849, 873, 973, 1035
Bme18I GGWCC 6 cut(s) 629, 821, 845, 869, 893, 1058
BmeRI GACNNNNNGTC 1 cut(s) 1129
BmeT110I CYCGRG 1 cut(s) 126
BmgT120I GGNCC 7 cut(s) 629, 821, 845, 869, 893, 1050, 1058
BmiI GGNNCC 2 cut(s) 134, 1105
BmrFI CCNGG 6 cut(s) 84, 633, 849, 873, 973, 1035
BmsI GCATC 3 cut(s) 208, 230, 1153
BplI GAGNNNNNCTC 2 cut(s) 519, 551
BpmI CTGGAG 2 cut(s) 1041, 1107
Bpu1102I GCTNAGC 1 cut(s) 393
Bpu14I TTCGAA 1 cut(s) 779
BpuEI CTTGAG 2 cut(s) 464, 593
BsaI GGTCTC 1 cut(s) 706
BsaJI CCNNGG 4 cut(s) 264, 632, 657, 971
BsaWI WCCGGW 1 cut(s) 665
Bsc4I CCNNNNNNNGG 8 cut(s) 89, 672, 818, 842, 866, 890, 1034, 1040
Bse118I RCCGGY 1 cut(s) 1100
Bse1I ACTGG 1 cut(s) 239
Bse3DI GCAATG 1 cut(s) 1077
BseBI CCWGG 6 cut(s) 84, 633, 849, 873, 973, 1035
BseDI CCNNGG 4 cut(s) 264, 632, 657, 971
BseGI GGATG 1 cut(s) 54
BseLI CCNNNNNNNGG 8 cut(s) 89, 672, 818, 842, 866, 890, 1034, 1040
BseMI GCAATG 1 cut(s) 1077
BseMII CTCAG 3 cut(s) 355, 402, 503
BseNI ACTGG 1 cut(s) 239
BseRI GAGGAG 1 cut(s) 909
BseSI GKGCMC 1 cut(s) 1011
BseXI GCAGC 1 cut(s) 1109
BsgI GTGCAG 3 cut(s) 190, 290, 663
Bsh1236I CGCG 1 cut(s) 60
BshFI GGCC 1 cut(s) 1052
BshNI GGYRCC 1 cut(s) 1103
BsiHKAI GWGCWC 1 cut(s) 1027
BsiHKCI CYCGRG 1 cut(s) 126
BsiSI CCGG 4 cut(s) 136, 666, 1053, 1101
BslFI GGGAC 2 cut(s) 133, 373
BslI CCNNNNNNNGG 8 cut(s) 89, 672, 818, 842, 866, 890, 1034, 1040
BsmAI GTCTC 1 cut(s) 706
BsmFI GGGAC 2 cut(s) 133, 373
BsnI GGCC 1 cut(s) 1052
Bso31I GGTCTC 1 cut(s) 706
BsoBI CYCGRG 1 cut(s) 126
Bsp119I TTCGAA 1 cut(s) 779
Bsp1286I GDGCHC 3 cut(s) 135, 1011, 1027
Bsp143I GATC 5 cut(s) 567, 637, 979, 1110, 1186
Bsp1720I GCTNAGC 1 cut(s) 393
BspACI CCGC 3 cut(s) 819, 867, 926
BspANI GGCC 1 cut(s) 1052
BspCNI CTCAG 3 cut(s) 354, 403, 504
BspFNI CGCG 1 cut(s) 60
BspLI GGNNCC 2 cut(s) 134, 1105
BspMAI CTGCAG 1 cut(s) 793
BspMI ACCTGC 4 cut(s) 53, 864, 888, 1069
BspPI GGATC 3 cut(s) 562, 645, 1181
BspT104I TTCGAA 1 cut(s) 779
BspT107I GGYRCC 1 cut(s) 1103
BspTNI GGTCTC 1 cut(s) 706
BsrDI GCAATG 1 cut(s) 1077
BsrFI RCCGGY 1 cut(s) 1100
BsrI ACTGG 1 cut(s) 239
BssAI RCCGGY 1 cut(s) 1100
BssECI CCNNGG 4 cut(s) 264, 632, 657, 971
BssMI GATC 5 cut(s) 567, 637, 979, 1110, 1186
BssNAI GTATAC 1 cut(s) 9
BssT1I CCWWGG 2 cut(s) 264, 657
Bst1107I GTATAC 1 cut(s) 9
Bst2UI CCWGG 6 cut(s) 84, 633, 849, 873, 973, 1035
Bst4CI ACNGT 1 cut(s) 545
BstAPI GCANNNNNTGC 1 cut(s) 1103
BstBI TTCGAA 1 cut(s) 779
BstC8I GCNNGC 6 cut(s) 817, 837, 861, 865, 885, 1095
BstDEI CTNAG 4 cut(s) 341, 393, 411, 512
BstF5I GGATG 1 cut(s) 54
BstFNI CGCG 1 cut(s) 60
BstH2I RGCGCY 1 cut(s) 1094
BstHHI GCGC 2 cut(s) 62, 1093
BstKTI GATC 5 cut(s) 570, 640, 982, 1113, 1189
BstMAI GTCTC 1 cut(s) 706
BstMBI GATC 5 cut(s) 567, 637, 979, 1110, 1186
BstMWI GCNNNNNNNGC 4 cut(s) 825, 873, 1097, 1103
BstNI CCWGG 6 cut(s) 84, 633, 849, 873, 973, 1035
BstSCI CCNGG 6 cut(s) 82, 631, 847, 871, 971, 1033
BstSFI CTRYAG 3 cut(s) 319, 353, 789
BstSLI GKGCMC 1 cut(s) 1011
BstUI CGCG 1 cut(s) 60
BstV1I GCAGC 1 cut(s) 1109
BstZ17I GTATAC 1 cut(s) 9
BsuRI GGCC 1 cut(s) 1052
BtsCI GGATG 1 cut(s) 54
BveI ACCTGC 4 cut(s) 53, 864, 888, 1069
Cac8I GCNNGC 6 cut(s) 817, 837, 861, 865, 885, 1095
CfoI GCGC 2 cut(s) 62, 1093
Cfr10I RCCGGY 1 cut(s) 1100
Cfr13I GGNCC 7 cut(s) 629, 821, 845, 869, 893, 1050, 1058
CseI GACGC 1 cut(s) 284
Csp6I GTAC 2 cut(s) 202, 956
CviAII CATG 1 cut(s) 425
CviQI GTAC 2 cut(s) 202, 956
DdeI CTNAG 4 cut(s) 341, 393, 411, 512
DpnI GATC 5 cut(s) 569, 639, 981, 1112, 1188
DpnII GATC 5 cut(s) 567, 637, 979, 1110, 1186
DriI GACNNNNNGTC 1 cut(s) 1129
Eam1105I GACNNNNNGTC 1 cut(s) 1129
Eco130I CCWWGG 2 cut(s) 264, 657
Eco24I GRGCYC 1 cut(s) 135
Eco31I GGTCTC 1 cut(s) 706
Eco32I GATATC 1 cut(s) 120
Eco47I GGWCC 6 cut(s) 629, 821, 845, 869, 893, 1058
Eco47III AGCGCT 1 cut(s) 1092
Eco57I CTGAAG 1 cut(s) 1134
Eco88I CYCGRG 1 cut(s) 126
EcoO109I RGGNCCY 2 cut(s) 629, 1058
EcoRI GAATTC 4 cut(s) 406, 415, 465, 736
EcoRII CCWGG 6 cut(s) 82, 631, 847, 871, 971, 1033
EcoRV GATATC 1 cut(s) 120
EcoT14I CCWWGG 2 cut(s) 264, 657
EcoT22I ATGCAT 1 cut(s) 223
EcoT38I GRGCYC 1 cut(s) 135
ErhI CCWWGG 2 cut(s) 264, 657
FaeI CATG 1 cut(s) 428
FalI AAGNNNNNCTT 2 cut(s) 593, 625
FaqI GGGAC 2 cut(s) 133, 373
FatI CATG 1 cut(s) 424
FauI CCCGC 1 cut(s) 933
FblI GTMKAC 1 cut(s) 8
Fnu4HI GCNGC 1 cut(s) 1098
FokI GGATG 1 cut(s) 61
FriOI GRGCYC 1 cut(s) 135
Fsp4HI GCNGC 1 cut(s) 1098
FspBI CTAG 4 cut(s) 21, 912, 1047, 1167
GlaI GCGC 2 cut(s) 61, 1092
GluI GCNGC 1 cut(s) 1098
GsuI CTGGAG 2 cut(s) 1041, 1107
HaeII RGCGCY 1 cut(s) 1094
HaeIII GGCC 1 cut(s) 1052
HapII CCGG 4 cut(s) 136, 666, 1053, 1101
HgaI GACGC 1 cut(s) 284
HhaI GCGC 2 cut(s) 62, 1093
Hin1II CATG 1 cut(s) 428
Hin6I GCGC 2 cut(s) 60, 1091
HinP1I GCGC 2 cut(s) 60, 1091
HindIII AAGCTT 1 cut(s) 158
HinfI GANTC 2 cut(s) 281, 577
HpaII CCGG 4 cut(s) 136, 666, 1053, 1101
HphI GGTGA 3 cut(s) 572, 646, 1130
Hpy166II GTNNAC 3 cut(s) 9, 183, 893
Hpy188I TCNGA 3 cut(s) 344, 412, 513
Hpy188III TCNNGA 6 cut(s) 38, 225, 376, 912, 1020, 1134
Hpy8I GTNNAC 3 cut(s) 9, 183, 893
Hpy99I CGWCG 1 cut(s) 148
HpyAV CCTTC 4 cut(s) 517, 918, 948, 1158
HpyCH4III ACNGT 1 cut(s) 545
HpyCH4V TGCA 8 cut(s) 207, 221, 233, 307, 680, 749, 791, 1070
HpyF10VI GCNNNNNNNGC 4 cut(s) 825, 873, 1097, 1103
HpyF3I CTNAG 4 cut(s) 341, 393, 411, 512
Hsp92II CATG 1 cut(s) 428
HspAI GCGC 2 cut(s) 60, 1091
Kzo9I GATC 5 cut(s) 567, 637, 979, 1110, 1186
LmnI GCTCC 4 cut(s) 138, 444, 1022, 1088
Lsp1109I GCAGC 1 cut(s) 1109
LweI GCATC 3 cut(s) 208, 230, 1153
MaeI CTAG 4 cut(s) 21, 912, 1047, 1167
MaeIII GTNAC 3 cut(s) 52, 803, 1171
MalI GATC 5 cut(s) 569, 639, 981, 1112, 1188
MboI GATC 5 cut(s) 567, 637, 979, 1110, 1186
MboII GAAGA 3 cut(s) 53, 446, 1105
MfeI CAATTG 1 cut(s) 452
MhlI GDGCHC 3 cut(s) 135, 1011, 1027
MluCI AATT 6 cut(s) 406, 415, 452, 465, 725, 736
MmeI TCCRAC 3 cut(s) 494, 775, 928
Mph1103I ATGCAT 1 cut(s) 223
MseI TTAA 1 cut(s) 555
MslI CAYNNNNRTG 1 cut(s) 588
MspA1I CMGCKG 1 cut(s) 1184
MspI CCGG 4 cut(s) 136, 666, 1053, 1101
MspR9I CCNGG 6 cut(s) 84, 633, 849, 873, 973, 1035
MunI CAATTG 1 cut(s) 452
MvaI CCWGG 6 cut(s) 84, 633, 849, 873, 973, 1035
MvnI CGCG 1 cut(s) 60
MwoI GCNNNNNNNGC 4 cut(s) 825, 873, 1097, 1103
NdeII GATC 5 cut(s) 567, 637, 979, 1110, 1186
NlaIII CATG 1 cut(s) 428
NlaIV GGNNCC 2 cut(s) 134, 1105
NsiI ATGCAT 1 cut(s) 223
NspV TTCGAA 1 cut(s) 779
PaeR7I CTCGAG 1 cut(s) 126
PcsI WCGNNNNNNNCGW 1 cut(s) 64
PfeI GAWTC 2 cut(s) 281, 577
PflMI CCANNNNNTGG 2 cut(s) 890, 1034
PkrI GCNGC 1 cut(s) 1099
PpuMI RGGWCCY 2 cut(s) 629, 1058
Psp5II RGGWCCY 2 cut(s) 629, 1058
Psp6I CCWGG 6 cut(s) 82, 631, 847, 871, 971, 1033
PspGI CCWGG 6 cut(s) 82, 631, 847, 871, 971, 1033
PspN4I GGNNCC 2 cut(s) 134, 1105
PspPI GGNCC 7 cut(s) 629, 821, 845, 869, 893, 1050, 1058
PspPPI RGGWCCY 2 cut(s) 629, 1058
PspXI VCTCGAGB 1 cut(s) 126
PstI CTGCAG 1 cut(s) 793
PvuII CAGCTG 1 cut(s) 1184
RsaI GTAC 2 cut(s) 203, 957
RsaNI GTAC 2 cut(s) 202, 956
RseI CAYNNNNRTG 1 cut(s) 588
SaqAI TTAA 1 cut(s) 555
SatI GCNGC 1 cut(s) 1098
Sau3AI GATC 5 cut(s) 567, 637, 979, 1110, 1186
Sau96I GGNCC 7 cut(s) 629, 821, 845, 869, 893, 1050, 1058
ScaI AGTACT 1 cut(s) 203
ScrFI CCNGG 6 cut(s) 84, 633, 849, 873, 973, 1035
SduI GDGCHC 3 cut(s) 135, 1011, 1027
SfaNI GCATC 3 cut(s) 208, 230, 1153
SfcI CTRYAG 3 cut(s) 319, 353, 789
Sfr274I CTCGAG 1 cut(s) 126
SfuI TTCGAA 1 cut(s) 779
SinI GGWCC 6 cut(s) 629, 821, 845, 869, 893, 1058
SlaI CTCGAG 1 cut(s) 126
SmiMI CAYNNNNRTG 1 cut(s) 588
SmlI CTYRAG 3 cut(s) 126, 479, 572
SmoI CTYRAG 3 cut(s) 126, 479, 572
Sse9I AATT 6 cut(s) 406, 415, 452, 465, 725, 736
SsiI CCGC 3 cut(s) 819, 867, 926
SspMI CTAG 4 cut(s) 21, 912, 1047, 1167
StyD4I CCNGG 6 cut(s) 82, 631, 847, 871, 971, 1033
StyI CCWWGG 2 cut(s) 264, 657
TaaI ACNGT 1 cut(s) 545
TaqI TCGA 5 cut(s) 39, 67, 127, 764, 779
TasI AATT 6 cut(s) 406, 415, 452, 465, 725, 736
TatI WGTACW 1 cut(s) 201
TfiI GAWTC 2 cut(s) 281, 577
Tru1I TTAA 1 cut(s) 555
Tru9I TTAA 1 cut(s) 555
TseI GCWGC 1 cut(s) 1097
TspDTI ATGAA 2 cut(s) 609, 749
TspGWI ACGGA 2 cut(s) 61, 858
Van91I CCANNNNNTGG 2 cut(s) 890, 1034
VpaK11BI GGWCC 6 cut(s) 629, 821, 845, 869, 893, 1058
XapI RAATTY 4 cut(s) 406, 415, 465, 736
XbaI TCTAGA 1 cut(s) 911
XhoI CTCGAG 1 cut(s) 126
XmiI GTMKAC 1 cut(s) 8
XspI CTAG 4 cut(s) 21, 912, 1047, 1167
ZrmI AGTACT 1 cut(s) 203
Zsp2I ATGCAT 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.