Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0015694

Physical Location & Seq

Genomic Coordinates Forward (+)

6525663 .. 6529000

Gene Structure 3338 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00285090.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1968 bp

ATGGTTCCCTCACGTCTATTCCTTTCTCCCATTTTGTTACTGATCATAATCACTAGTACCACCGATCAAGCGGCCGCCCTTGCTCAGCCGCAGCCAGCAGCTGATCATCTGCTAAGCCACTTCTGTTCAAACCACAAAGGTAACTACACCACGACGGGTGCCTATCGCACAAACCTTAACCACCTTCTCTCATCCCTTCCCTTCAAGGGTAGCGGCTACGGCTTTTATAGTTCTTCATATGGCCGAAACACTACATCCGGCGCAATATATGCAATCGGACTATGTAAGGGAGACATTGGAACCACGCGCCACTGCCTTCATTGCCTCAATACCTCTAGAAGTGCTCTCATGGAGCTTTGTGCCAATCAGAAGGAAGCTATTGTTTGGTACGACAACTGCATGCTGCGCTACTCAAACCGCTCCCTATATGGCGTCATGGACACGCTGCCTGCTTTCGATTTGTGGAGCACTCAAAGCGTACCATCATCGGAGTTTGATGTATTTTTCCGGCAGCTCAGGTGGTTACTAGAAGCCCTAAGAAACAAAGCTGCGGCAGGGTGCACTCCTGATTTGTCCGAGTTACAGTGCAGCACTTGCTTACTTGGTGCTTTTGAAGAACTCCCAAAATGTTGTGATGGAAAGGAAGGTGGGCGAGTTCTTAGACCCAGCTGTAACTTCAACTATCAGCTGAACCAGGTTACTGAGGTCAATGAATTACCGAAAGGTTCCATGATTCCCAACTTAGGACGTCGTGCAAGAGATAGAAGAGTCGGCATCCTTATACTATTTGCAATGGTTGTTTCTCTTGTACTATTGATCTCCATAGGCATTTACGCGAAAGTAGTGAAGACAAGGCCACAACCTATCTTAGGGATGAAACATAATTTTATTGAGAAAATGGATGAGCATCTGGCCATGGAATCCTTGCTATTCGACTGTGGCGCCATTCGAGTTGCCACAAATAACTTTTCTGAAGCAAATAAACTTGGAGATGATAGATATGGCACTGTTTACAGGGGTAGTCTTGCCAACGCAAAAAGCATAGCAGTGAGAAGGCTTTCAATAAACTATGTGAAAGAGTATATGGAAATTAAGAAAGAGGCTTTGTTAATGGCCAAGCTTCAACATCCAAATCTAATTGAGCTCCTAGGGTGTGCCTTGGAAGAAAATTCTGAAAGGCTTCTTGTCTATGAGCTTTTCGCTAACGCAACTCTTGATCGCATCATCTTTGACGGAATAAAGAGCAGACAATGGGATTGGGAGAGCCGGTACAACATCATAGTGAGCATTGCACGGGGACTCCTTCACCTTCATGACCGTCACTTTGTTCATGGTGATGTCAATGCTGGTAACATTTTGATAGATGCACAAATGAATCCCAAAATTTCAAATTTTGGCATGGCAAGATTCTTTGAGGTTGATCAAACACAAGGCAACGTTAATGGAATTCATCTGCTGAATCTGATGTCTAACTCATACATGGCTCCAGAGGCAACTGTTGGACATTCATCTGCTGAATCTGATGTCTACAGTTTCGGTGCGTTGGTCGTGGAGATAGTAAGTGGAAAGGAAATTAGTAGTCTAACTTCCGAAAAGCGAAATGAGAATGATCTTCTGAGCTATGCATGGAAGAATTGGGCAAAAGGGACAATTAGAAATCTAATAGATCCGACGTTGTGGACTGGTTCAGGGATTGAATTAATGGAGAGATGCATCAGAATCGGATTATTCTGTATGCAGAGGACTAGAACCGATAGGCCAACCATGTCTTCTGTTATTCAGATGCTTAGTTATTCTTCCGCTATCCCTCTACCTTATAAATTTAGTCATGGTAGCTTTGGAATCATTGGAACATTGGAAAATGTGGAAGGTGTTTCTGATCCAAGGTCTAGGGCAAAGGTTAAATCCTTCATTGCAAAGATGCGGTGTGCCACTACTGCCATCATCAATAGGATACGTAGTAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0033993 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0071704 GO:0071944 GO:0097305 GO:0140096 GO:1901564 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr Stress-antifung

Protein Analysis

655

Amino Acids

73.13

Weight (kDa)

8.83

Isoelectric Point (pI)

41.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Stress-antifung	PF01657	42 - 140	2e-21	Salt stress response/antifungal
Pkinase	PF00069	324 - 594	9.5e-32	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	326 - 595	2.1e-35	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015694)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g25911
rosa_chinensis	RchiOBHm_Chr1g0372241 RchiOBHm_Chr1g0372291
rosa_laevigata	RLG00000026912 RLG00000026913 RLG00000026914
rosa_multiflora	Rmu_sc0001755.1_g000014 Rmu_sc0001755.1_g000017
rosa_roxburghii	Rroxscaffold_4G00285090 Rroxscaffold_4G00285120
rosa_rugosa	Rorug01G0370700
rosa_samantha	Rh1BG342500 Rh1CG356100 Rh1CG356200 Rh1DG373700 Rh1DG373900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1818
AatII	GACGTC	1 cut(s)	751
AccB1I	GGYRCC	2 cut(s)	158, 941
AccBSI	CCGCTC	1 cut(s)	420
AccI	GTMKAC	1 cut(s)	1527
AccII	CGCG	2 cut(s)	307, 836
AciI	CCGC	8 cut(s)	71, 75, 89, 213, 418, 551, 1800, 1924
AclI	AACGTT	1 cut(s)	1437
AclWI	GGATC	2 cut(s)	1661, 1874
AcoI	YGGCCR	4 cut(s)	72, 241, 912, 1113
AcsI	RAATTY	5 cut(s)	1168, 1383, 1390, 1446, 1820
AcuI	CTGAAG	1 cut(s)	993
AcyI	GRCGYC	3 cut(s)	432, 748, 942
AfaI	GTAC	5 cut(s)	58, 389, 480, 810, 1271
AfiI	CCNNNNNNNGG	3 cut(s)	206, 743, 869
AgsI	TTSAA	8 cut(s)	129, 205, 614, 679, 1062, 1124, 1389, 1697
AhlI	ACTAGT	1 cut(s)	53
AjiI	CACGTC	1 cut(s)	14
AjnI	CCWGG	1 cut(s)	693
AjuI	GAANNNNNNNTTGG	2 cut(s)	1753, 1785
Alw21I	GWGCWC	4 cut(s)	346, 470, 563, 1146
Alw26I	GTCTC	1 cut(s)	285
Alw44I	GTGCAC	1 cut(s)	559
AlwI	GGATC	2 cut(s)	1661, 1874
AlwNI	CAGNNNCTG	1 cut(s)	101
AoxI	GGCC	6 cut(s)	72, 241, 854, 912, 1113, 1757
ApaLI	GTGCAC	1 cut(s)	559
ApeKI	GCWGC	7 cut(s)	91, 98, 403, 445, 511, 548, 588
ApoI	RAATTY	5 cut(s)	1168, 1383, 1390, 1446, 1820
ArsI	GACNNNNNNTTYG	2 cut(s)	1307, 1339
AseI	ATTAAT	1 cut(s)	1700
Asp700I	GAANNNNTTC	2 cut(s)	1057, 1179
AspA2I	CCTAGG	1 cut(s)	1147
AspLEI	GCGC	4 cut(s)	263, 309, 408, 944
AsuHPI	GGTGA	2 cut(s)	1298, 1346
AvrII	CCTAGG	1 cut(s)	1147
BaeGI	GKGCMC	1 cut(s)	563
BalI	TGGCCA	2 cut(s)	914, 1115
BanI	GGYRCC	2 cut(s)	158, 941
BanII	GRGCYC	1 cut(s)	1146
BarI	GAAGNNNNNNTAC	2 cut(s)	1570, 1602
BbsI	GAAGAC	2 cut(s)	854, 1761
Bbv12I	GWGCWC	4 cut(s)	346, 470, 563, 1146
BbvI	GCAGC	7 cut(s)	103, 110, 390, 432, 523, 535, 600
BccI	CCATC	3 cut(s)	490, 629, 1949
BceAI	ACGGC	1 cut(s)	235
BcgI	CGANNNNNNTGC	2 cut(s)	1702, 1736
BciT130I	CCWGG	1 cut(s)	695
BciVI	GTATCC	1 cut(s)	1947
BclI	TGATCA	3 cut(s)	42, 103, 1420
BcoDI	GTCTC	1 cut(s)	285
BcuI	ACTAGT	1 cut(s)	53
BfaI	CTAG	6 cut(s)	54, 336, 527, 1148, 1746, 1890
BfmI	CTRYAG	1 cut(s)	1528
BfoI	RGCGCY	1 cut(s)	945
BfuI	GTATCC	1 cut(s)	1947
BlnI	CCTAGG	1 cut(s)	1147
BlpI	GCTNAGC	2 cut(s)	84, 113
Bme1390I	CCNGG	1 cut(s)	695
BmgBI	CACGTC	1 cut(s)	14
BmiI	GGNNCC	6 cut(s)	6, 160, 301, 727, 943, 1485
BmrFI	CCNGG	1 cut(s)	695
BmsI	GCATC	8 cut(s)	783, 916, 1230, 1354, 1700, 1722, 1773, 1911
BpiI	GAAGAC	2 cut(s)	854, 1761
BpmI	CTGGAG	1 cut(s)	1470
Bpu10I	CCTNAGC	1 cut(s)	515
Bpu1102I	GCTNAGC	2 cut(s)	84, 113
BsaAI	YACGTR	1 cut(s)	1958
BsaBI	GATNNNNATC	2 cut(s)	47, 906
BsaHI	GRCGYC	3 cut(s)	432, 748, 942
BsaJI	CCNNGG	4 cut(s)	915, 1147, 1158, 1883
BsaXI	ACNNNNNCTCC	4 cut(s)	1253, 1283, 1284, 1314
Bsc4I	CCNNNNNNNGG	3 cut(s)	206, 743, 869
Bse118I	RCCGGY	1 cut(s)	1266
Bse1I	ACTGG	1 cut(s)	1687
Bse3DI	GCAATG	4 cut(s)	319, 798, 1287, 1911
Bse8I	GATNNNNATC	2 cut(s)	47, 906
BseBI	CCWGG	1 cut(s)	695
BseDI	CCNNGG	4 cut(s)	915, 1147, 1158, 1883
BseGI	GGATG	6 cut(s)	191, 254, 774, 879, 907, 1126
BseJI	GATNNNNATC	2 cut(s)	47, 906
BseLI	CCNNNNNNNGG	3 cut(s)	206, 743, 869
BseMI	GCAATG	4 cut(s)	319, 798, 1287, 1911
BseMII	CTCAG	4 cut(s)	98, 529, 693, 1607
BseNI	ACTGG	1 cut(s)	1687
BseSI	GKGCMC	1 cut(s)	563
BseX3I	CGGCCG	1 cut(s)	72
BseXI	GCAGC	7 cut(s)	103, 110, 390, 432, 523, 535, 600
BseYI	CCCAGC	1 cut(s)	665
BsgI	GTGCAG	1 cut(s)	607
Bsh1236I	CGCG	2 cut(s)	307, 836
Bsh1285I	CGRYCG	1 cut(s)	75
BshFI	GGCC	6 cut(s)	74, 243, 856, 914, 1115, 1759
BshNI	GGYRCC	2 cut(s)	158, 941
BsiEI	CGRYCG	1 cut(s)	75
BsiHKAI	GWGCWC	4 cut(s)	346, 470, 563, 1146
BsiSI	CCGG	3 cut(s)	258, 508, 1267
BslFI	GGGAC	2 cut(s)	1311, 1660
BslI	CCNNNNNNNGG	3 cut(s)	206, 743, 869
BsmAI	GTCTC	1 cut(s)	285
BsmFI	GGGAC	2 cut(s)	1311, 1660
BsnI	GGCC	6 cut(s)	74, 243, 856, 914, 1115, 1759
Bsp1286I	GDGCHC	4 cut(s)	346, 470, 563, 1146
Bsp143I	GATC	9 cut(s)	42, 64, 103, 816, 1216, 1420, 1609, 1666, 1879
Bsp1720I	GCTNAGC	2 cut(s)	84, 113
Bsp19I	CCATGG	1 cut(s)	915
BspACI	CCGC	8 cut(s)	71, 75, 89, 213, 418, 551, 1800, 1924
BspANI	GGCC	6 cut(s)	74, 243, 856, 914, 1115, 1759
BspCNI	CTCAG	4 cut(s)	97, 528, 694, 1608
BspFNI	CGCG	2 cut(s)	307, 836
BspHI	TCATGA	1 cut(s)	1312
BspLI	GGNNCC	6 cut(s)	6, 160, 301, 727, 943, 1485
BspPI	GGATC	2 cut(s)	1661, 1874
BspT107I	GGYRCC	2 cut(s)	158, 941
BsrBI	CCGCTC	1 cut(s)	420
BsrDI	GCAATG	4 cut(s)	319, 798, 1287, 1911
BsrFI	RCCGGY	1 cut(s)	1266
BsrI	ACTGG	1 cut(s)	1687
BssAI	RCCGGY	1 cut(s)	1266
BssECI	CCNNGG	4 cut(s)	915, 1147, 1158, 1883
BssMI	GATC	9 cut(s)	42, 64, 103, 816, 1216, 1420, 1609, 1666, 1879
BssNI	GRCGYC	3 cut(s)	432, 748, 942
BssT1I	CCWWGG	4 cut(s)	915, 1147, 1158, 1883
Bst2UI	CCWGG	1 cut(s)	695
Bst4CI	ACNGT	6 cut(s)	585, 938, 1009, 1319, 1498, 1532
Bst6I	CTCTTC	1 cut(s)	760
BstACI	GRCGYC	3 cut(s)	432, 748, 942
BstAPI	GCANNNNNTGC	2 cut(s)	269, 594
BstBAI	YACGTR	1 cut(s)	1958
BstC8I	GCNNGC	3 cut(s)	96, 401, 450
BstDSI	CCRYGG	1 cut(s)	915
BstENI	CCTNNNNNAGG	1 cut(s)	867
BstF5I	GGATG	6 cut(s)	191, 254, 774, 879, 907, 1126
BstFNI	CGCG	2 cut(s)	307, 836
BstH2I	RGCGCY	1 cut(s)	945
BstHHI	GCGC	4 cut(s)	263, 309, 408, 944
BstKTI	GATC	9 cut(s)	45, 67, 106, 819, 1219, 1423, 1612, 1669, 1882
BstMAI	GTCTC	1 cut(s)	285
BstMBI	GATC	9 cut(s)	42, 64, 103, 816, 1216, 1420, 1609, 1666, 1879
BstMCI	CGRYCG	1 cut(s)	75
BstMWI	GCNNNNNNNGC	9 cut(s)	80, 219, 269, 321, 405, 474, 594, 1490, 1937
BstNI	CCWGG	1 cut(s)	695
BstNSI	RCATGY	1 cut(s)	403
BstSCI	CCNGG	1 cut(s)	693
BstSFI	CTRYAG	1 cut(s)	1528
BstSLI	GKGCMC	1 cut(s)	563
BstSNI	TACGTA	1 cut(s)	1958
BstUI	CGCG	2 cut(s)	307, 836
BstV1I	GCAGC	7 cut(s)	103, 110, 390, 432, 523, 535, 600
BstV2I	GAAGAC	2 cut(s)	854, 1761
BstX2I	RGATCY	1 cut(s)	1666
BstYI	RGATCY	1 cut(s)	1666
BstZI	CGGCCG	1 cut(s)	72
BsuI	GTATCC	1 cut(s)	1947
BsuRI	GGCC	6 cut(s)	74, 243, 856, 914, 1115, 1759
BtgI	CCRYGG	1 cut(s)	915
BtrI	CACGTC	1 cut(s)	14
BtsCI	GGATG	6 cut(s)	191, 254, 774, 879, 907, 1126
BtsI	GCAGTG	2 cut(s)	310, 1053
BtsIMutI	CAGTG	4 cut(s)	310, 590, 1005, 1053
Cac8I	GCNNGC	3 cut(s)	96, 401, 450
CaiI	CAGNNNCTG	1 cut(s)	101
CciI	TCATGA	1 cut(s)	1312
CciNI	GCGGCCGC	1 cut(s)	72
CfoI	GCGC	4 cut(s)	263, 309, 408, 944
Cfr10I	RCCGGY	1 cut(s)	1266
CseI	GACGC	1 cut(s)	421
CsiI	ACCWGGT	1 cut(s)	693
Csp6I	GTAC	5 cut(s)	57, 388, 479, 809, 1270
CviQI	GTAC	5 cut(s)	57, 388, 479, 809, 1270
DinI	GGCGCC	1 cut(s)	943
DpnI	GATC	9 cut(s)	44, 66, 105, 818, 1218, 1422, 1611, 1668, 1881
DpnII	GATC	9 cut(s)	42, 64, 103, 816, 1216, 1420, 1609, 1666, 1879
EaeI	YGGCCR	4 cut(s)	72, 241, 912, 1113
EagI	CGGCCG	1 cut(s)	72
Eam1104I	CTCTTC	1 cut(s)	760
EarI	CTCTTC	1 cut(s)	760
Ecl136II	GAGCTC	1 cut(s)	1144
EclXI	CGGCCG	1 cut(s)	72
Eco105I	TACGTA	1 cut(s)	1958
Eco130I	CCWWGG	4 cut(s)	915, 1147, 1158, 1883
Eco24I	GRGCYC	1 cut(s)	1146
Eco52I	CGGCCG	1 cut(s)	72
Eco53kI	GAGCTC	1 cut(s)	1144
Eco57I	CTGAAG	1 cut(s)	993
EcoICRI	GAGCTC	1 cut(s)	1144
EcoNI	CCTNNNNNAGG	1 cut(s)	867
EcoRI	GAATTC	1 cut(s)	1446
EcoRII	CCWGG	1 cut(s)	693
EcoT14I	CCWWGG	4 cut(s)	915, 1147, 1158, 1883
EcoT22I	ATGCAT	2 cut(s)	1627, 1715
EcoT38I	GRGCYC	1 cut(s)	1146
EgeI	GGCGCC	1 cut(s)	943
EheI	GGCGCC	1 cut(s)	943
ErhI	CCWWGG	4 cut(s)	915, 1147, 1158, 1883
FaqI	GGGAC	2 cut(s)	1311, 1660
FauNDI	CATATG	1 cut(s)	238
FbaI	TGATCA	3 cut(s)	42, 103, 1420
FblI	GTMKAC	1 cut(s)	1527
FokI	GGATG	6 cut(s)	178, 241, 761, 886, 914, 1113
FriOI	GRGCYC	1 cut(s)	1146
FspBI	CTAG	6 cut(s)	54, 336, 527, 1148, 1746, 1890
GlaI	GCGC	4 cut(s)	262, 308, 407, 943
GsaI	CCCAGC	1 cut(s)	669
GsuI	CTGGAG	1 cut(s)	1470
HaeII	RGCGCY	1 cut(s)	945
HaeIII	GGCC	6 cut(s)	74, 243, 856, 914, 1115, 1759
HapII	CCGG	3 cut(s)	258, 508, 1267
HgaI	GACGC	1 cut(s)	421
HhaI	GCGC	4 cut(s)	263, 309, 408, 944
Hin1I	GRCGYC	3 cut(s)	432, 748, 942
Hin6I	GCGC	4 cut(s)	261, 307, 406, 942
HinP1I	GCGC	4 cut(s)	261, 307, 406, 942
HindIII	AAGCTT	1 cut(s)	1118
HpaII	CCGG	3 cut(s)	258, 508, 1267
HphI	GGTGA	2 cut(s)	1298, 1346
Hpy166II	GTNNAC	4 cut(s)	561, 1012, 1528, 1680
Hpy188III	TCNNGA	5 cut(s)	336, 566, 1214, 1313, 1487
Hpy8I	GTNNAC	4 cut(s)	561, 1012, 1528, 1680
Hpy99I	CGWCG	3 cut(s)	157, 753, 1675
HpyCH4III	ACNGT	6 cut(s)	585, 938, 1009, 1319, 1498, 1532
HpyCH4IV	ACGT	5 cut(s)	13, 748, 1437, 1673, 1957
HpyF10VI	GCNNNNNNNGC	9 cut(s)	80, 219, 269, 321, 405, 474, 594, 1490, 1937
HpySE526I	ACGT	5 cut(s)	13, 748, 1437, 1673, 1957
Hsp92I	GRCGYC	3 cut(s)	432, 748, 942
HspAI	GCGC	4 cut(s)	261, 307, 406, 942
KasI	GGCGCC	1 cut(s)	941
Ksp22I	TGATCA	3 cut(s)	42, 103, 1420
Kzo9I	GATC	9 cut(s)	42, 64, 103, 816, 1216, 1420, 1609, 1666, 1879
LmnI	GCTCC	5 cut(s)	352, 425, 465, 1149, 1489
Lsp1109I	GCAGC	7 cut(s)	103, 110, 390, 432, 523, 535, 600
LweI	GCATC	8 cut(s)	783, 916, 1230, 1354, 1700, 1722, 1773, 1911
MabI	ACCWGGT	1 cut(s)	693
MaeI	CTAG	6 cut(s)	54, 336, 527, 1148, 1746, 1890
MaeII	ACGT	5 cut(s)	13, 748, 1437, 1673, 1957
MaeIII	GTNAC	8 cut(s)	36, 140, 522, 579, 671, 697, 1319, 1349
MalI	GATC	9 cut(s)	44, 66, 105, 818, 1218, 1422, 1611, 1668, 1881
MbiI	CCGCTC	1 cut(s)	420
MboI	GATC	9 cut(s)	42, 64, 103, 816, 1216, 1420, 1609, 1666, 1879
MboII	GAAGA	9 cut(s)	225, 626, 777, 859, 1175, 1604, 1642, 1761, 1788
MflI	RGATCY	1 cut(s)	1666
MhlI	GDGCHC	4 cut(s)	346, 470, 563, 1146
MlsI	TGGCCA	2 cut(s)	914, 1115
MluNI	TGGCCA	2 cut(s)	914, 1115
Mly113I	GGCGCC	1 cut(s)	942
MlyI	GAGTC	2 cut(s)	777, 1293
MmeI	TCCRAC	2 cut(s)	1480, 1694
MnlI	CCTC	9 cut(s)	19, 335, 343, 697, 1093, 1408, 1483, 1734, 1818
Mox20I	TGGCCA	2 cut(s)	914, 1115
Mph1103I	ATGCAT	2 cut(s)	1627, 1715
MroXI	GAANNNNTTC	2 cut(s)	1057, 1179
MscI	TGGCCA	2 cut(s)	914, 1115
MseI	TTAA	6 cut(s)	177, 1092, 1109, 1440, 1700, 1902
MslI	CAYNNNNRTG	4 cut(s)	1046, 1280, 1311, 1335
Msp20I	TGGCCA	2 cut(s)	914, 1115
MspA1I	CMGCKG	3 cut(s)	101, 669, 688
MspI	CCGG	3 cut(s)	258, 508, 1267
MspR9I	CCNGG	1 cut(s)	695
MvaI	CCWGG	1 cut(s)	695
MvnI	CGCG	2 cut(s)	307, 836
MwoI	GCNNNNNNNGC	9 cut(s)	80, 219, 269, 321, 405, 474, 594, 1490, 1937
NarI	GGCGCC	1 cut(s)	942
NcoI	CCATGG	1 cut(s)	915
NdeI	CATATG	1 cut(s)	238
NdeII	GATC	9 cut(s)	42, 64, 103, 816, 1216, 1420, 1609, 1666, 1879
NlaIV	GGNNCC	6 cut(s)	6, 160, 301, 727, 943, 1485
NmuCI	GTSAC	1 cut(s)	1319
NotI	GCGGCCGC	1 cut(s)	72
NsiI	ATGCAT	2 cut(s)	1627, 1715
NspI	RCATGY	1 cut(s)	403
PaeI	GCATGC	1 cut(s)	403
PagI	TCATGA	1 cut(s)	1312
PdmI	GAANNNNTTC	2 cut(s)	1057, 1179
PfeI	GAWTC	8 cut(s)	733, 920, 1375, 1407, 1459, 1517, 1719, 1842
PleI	GAGTC	2 cut(s)	776, 1293
PluTI	GGCGCC	1 cut(s)	945
PpsI	GAGTC	2 cut(s)	776, 1293
Ppu21I	YACGTR	1 cut(s)	1958
PshBI	ATTAAT	1 cut(s)	1700
PsiI	TTATAA	1 cut(s)	1818
Psp124BI	GAGCTC	1 cut(s)	1146
Psp1406I	AACGTT	1 cut(s)	1437
Psp6I	CCWGG	1 cut(s)	693
PspFI	CCCAGC	1 cut(s)	665
PspGI	CCWGG	1 cut(s)	693
PspN4I	GGNNCC	6 cut(s)	6, 160, 301, 727, 943, 1485
PstNI	CAGNNNCTG	1 cut(s)	101
PsuI	RGATCY	1 cut(s)	1666
PvuII	CAGCTG	3 cut(s)	101, 669, 688
RsaI	GTAC	5 cut(s)	58, 389, 480, 810, 1271
RsaNI	GTAC	5 cut(s)	57, 388, 479, 809, 1270
RseI	CAYNNNNRTG	4 cut(s)	1046, 1280, 1311, 1335
SacI	GAGCTC	1 cut(s)	1146
SaqAI	TTAA	6 cut(s)	177, 1092, 1109, 1440, 1700, 1902
Sau3AI	GATC	9 cut(s)	42, 64, 103, 816, 1216, 1420, 1609, 1666, 1879
SchI	GAGTC	2 cut(s)	777, 1293
ScrFI	CCNGG	1 cut(s)	695
SduI	GDGCHC	4 cut(s)	346, 470, 563, 1146
SexAI	ACCWGGT	1 cut(s)	693
SfaNI	GCATC	8 cut(s)	783, 916, 1230, 1354, 1700, 1722, 1773, 1911
SfcI	CTRYAG	1 cut(s)	1528
SfoI	GGCGCC	1 cut(s)	943
SmiMI	CAYNNNNRTG	4 cut(s)	1046, 1280, 1311, 1335
SnaBI	TACGTA	1 cut(s)	1958
SpeI	ACTAGT	1 cut(s)	53
SphI	GCATGC	1 cut(s)	403
SsiI	CCGC	8 cut(s)	71, 75, 89, 213, 418, 551, 1800, 1924
SspDI	GGCGCC	1 cut(s)	941
SspMI	CTAG	6 cut(s)	54, 336, 527, 1148, 1746, 1890
SstI	GAGCTC	1 cut(s)	1146
StyD4I	CCNGG	1 cut(s)	693
StyI	CCWWGG	4 cut(s)	915, 1147, 1158, 1883
TaaI	ACNGT	6 cut(s)	585, 938, 1009, 1319, 1498, 1532
TaiI	ACGT	5 cut(s)	16, 751, 1440, 1676, 1960
TaqI	TCGA	3 cut(s)	456, 933, 949
TatI	WGTACW	1 cut(s)	808
TauI	GCSGC	5 cut(s)	74, 77, 91, 216, 554
TfiI	GAWTC	8 cut(s)	733, 920, 1375, 1407, 1459, 1517, 1719, 1842
Tru1I	TTAA	6 cut(s)	177, 1092, 1109, 1440, 1700, 1902
Tru9I	TTAA	6 cut(s)	177, 1092, 1109, 1440, 1700, 1902
TscAI	CASTG	4 cut(s)	317, 590, 1012, 1053
TseFI	GTSAC	1 cut(s)	1319
TseI	GCWGC	7 cut(s)	91, 98, 403, 445, 511, 548, 588
Tsp45I	GTSAC	1 cut(s)	1319
TspGWI	ACGGA	1 cut(s)	1248
TspRI	CASTG	4 cut(s)	317, 590, 1012, 1053
VneI	GTGCAC	1 cut(s)	559
VspI	ATTAAT	1 cut(s)	1700
XagI	CCTNNNNNAGG	1 cut(s)	867
XapI	RAATTY	5 cut(s)	1168, 1383, 1390, 1446, 1820
XbaI	TCTAGA	1 cut(s)	335
XceI	RCATGY	1 cut(s)	403
XmaJI	CCTAGG	1 cut(s)	1147
XmiI	GTMKAC	1 cut(s)	1527
XmnI	GAANNNNTTC	2 cut(s)	1057, 1179
XspI	CTAG	6 cut(s)	54, 336, 527, 1148, 1746, 1890
ZraI	GACGTC	1 cut(s)	749
Zsp2I	ATGCAT	2 cut(s)	1627, 1715

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.