Rroxscaffold_4G00289650

Dolichyl-phosphate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0011278
Physical Location & Seq
Forward (+)
10220924 .. 10224566
3643 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00289650.1

Sequence Viewer

Length: 1182 bp
ATGGAGATCGTAATCGTATGGACGATGGTAGAGCTTCTGCTAGCCCTCATTCTCATCGTAGCCCTAGGTTTCGTCTCCGCTGTCTTCTTCGAAGCTCATAGAAGACGCCACAACAACGCGCACGTCGAAGCTCCGGCGATCTGCGAAGACCCGAAGTCGAGGAAACAGGTTACATGCCCTTCGATTTACGATCCGGCGGAGAAGTATCTTTCTTTGATAATCCCTGCATTCAATGAAGAGCTTAGGCTTCCTCTGGCCCTTGAGGAAACAATGAATTATCTCCAACAACGCGCTGCCAAGGATAAGTCATTTTCTTATGAGGTGGTTATCGTTGATGATGGAAGTGTTGATGGGACAAAAAGAGTAGCATATGAATTTGTAAAGAAATACACTGTAGACAATGTGAGGGTTATCCTGCTTGGAAGAAATCATGGAAAGGGAGAGGCTATCAGAAAAGGAATGCTTCATTCAAGGGGTGAGCTACTTCTAATGCTGGATGCAGATGGAGCAACAAAGGTTGATGATGTAGAAAAACTTGAAAAAAAGATCCATGCAGTTGCAAGTGAGGAATTTAAGTTTGGGGACTCACCTGCTAGTAACTCAGATTTTAGAATATCTGATATCCCAATTGCTGCATTTGGTTCCCGTGCTCATCTCGAGGAGAAGGCCTTGGCTTCAAGGAAGTGGTACCGCAATTTCTTGATGAAGGGTTTCCATCTTGTGGTTCTTTTGGCTGCTGGTCCTGGAATTCGTGATACCCAGTGTGGTTTTAAGATGTTCACTAGGGCTGCTGCAAGGAAGCTCTTCACTAACATCCGTTTGAAGAGGTGGTGCTTTGATGTTGAGTTGGTTTTCCTAGGCAATCGGTTTCGAATTCCGATGCGTGAGATATCTGTGAACTGGTCTGAAATTCCTGGGTCAAAGGTGAACTTATTAAGCATTCCAAATATGCTTTGGGAGCTTGTGCTGATGTCTGTTGGATATAGAAGCGGTGCTAAACTTTTTCAGAAGCATTGCCGCCCTCCTGGCCTCTATACATCAGCAGTAGAAATCAAATATCTCATCAGATATAGAGGGTTTTATACTTTGTTTCGGGATGTTAATTACAAAAATAAACTATTAAATAATAAAATAAATTATCCGCTGATAGTGTCACGAGTCACGACATGCCTAAAATGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00510 ko01100 map00510 map01100
Pfam Domains
Protein Families
Glycos_transf_2

Protein Analysis

393

Amino Acids

44.87

Weight (kDa)

9.46

Isoelectric Point (pI)

29.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glycos_transf_2 PF00535 71 - 184 2.1e-22 Glycosyl transferase family 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011278)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39630
fragaria_vesca FvH4_7g22450 FvH4_7g22450
malus_domestica MD01G1133900.v1.1 MD07G1198100.v1.1
prunus_persica Prupe.2G235200_v2.0.a1
pyrus_communis pycom01g15470 pycom07g18530
rosa_chinensis RchiOBHm_Chr1g0367061
rosa_laevigata RLG00000027302
rosa_multiflora Rmu_sc0037952.1_g000001 Rmu_sc0037953.1_g000001
rosa_roxburghii Rroxscaffold_4G00289650
rosa_rugosa Rorug01G0334200 Rorug01G0334300
rosa_samantha Rh1AG341100 Rh1BG301600 Rh1CG317800 Rh1DG334000
rosa_wichuraiana Rw1G030300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 598
Acc36I ACCTGC 1 cut(s) 598
Acc65I GGTACC 1 cut(s) 687
AccB1I GGYRCC 1 cut(s) 687
AccB7I CCANNNNNTGG 1 cut(s) 721
AccI GTMKAC 1 cut(s) 396
AccII CGCG 2 cut(s) 119, 291
AciI CCGC 6 cut(s) 78, 197, 691, 990, 1018, 1142
AclWI GGATC 2 cut(s) 185, 541
AcsI RAATTY 5 cut(s) 374, 569, 747, 873, 909
AcyI GRCGYC 1 cut(s) 106
AfaI GTAC 1 cut(s) 689
AfiI CCNNNNNNNGG 1 cut(s) 721
AgsI TTSAA 5 cut(s) 232, 471, 539, 678, 823
AhdI GACNNNNNGTC 1 cut(s) 154
AjiI CACGTC 1 cut(s) 124
AjnI CCWGG 3 cut(s) 742, 913, 1024
AjuI GAANNNNNNNTTGG 2 cut(s) 561, 593
AluBI AGCT 7 cut(s) 34, 95, 131, 241, 481, 802, 961
AluI AGCT 7 cut(s) 34, 95, 131, 241, 481, 802, 961
Alw21I GWGCWC 1 cut(s) 652
Alw26I GTCTC 1 cut(s) 79
AlwI GGATC 2 cut(s) 185, 541
Ama87I CYCGRG 1 cut(s) 656
AoxI GGCC 3 cut(s) 255, 666, 1027
ApeKI GCWGC 5 cut(s) 293, 632, 734, 788, 791
ApoI RAATTY 5 cut(s) 374, 569, 747, 873, 909
Asp700I GAANNNNTTC 2 cut(s) 710, 803
Asp718I GGTACC 1 cut(s) 687
AspA2I CCTAGG 2 cut(s) 64, 856
AspLEI GCGC 2 cut(s) 121, 293
AspS9I GGNCC 2 cut(s) 256, 740
AsuHPI GGTGA 3 cut(s) 488, 579, 937
AsuII TTCGAA 2 cut(s) 90, 871
AsuNHI GCTAGC 1 cut(s) 40
AvaI CYCGRG 1 cut(s) 656
AvaII GGWCC 1 cut(s) 740
AvrII CCTAGG 2 cut(s) 64, 856
BanI GGYRCC 1 cut(s) 687
BauI CACGAG 1 cut(s) 1155
BbsI GAAGAC 3 cut(s) 76, 109, 153
Bbv12I GWGCWC 1 cut(s) 652
BbvI GCAGC 5 cut(s) 280, 619, 721, 775, 778
BccI CCATC 5 cut(s) 19, 332, 344, 497, 723
BciT130I CCWGG 3 cut(s) 744, 915, 1026
BcoDI GTCTC 1 cut(s) 79
BfaI CTAG 5 cut(s) 41, 65, 594, 783, 857
BfmI CTRYAG 1 cut(s) 393
BfuAI ACCTGC 1 cut(s) 598
BglI GCCNNNNNGGC 1 cut(s) 1026
BisI GCNGC 6 cut(s) 294, 633, 735, 789, 792, 1018
BlnI CCTAGG 2 cut(s) 64, 856
BlsI GCNGC 6 cut(s) 295, 634, 736, 790, 793, 1019
Bme1390I CCNGG 3 cut(s) 744, 915, 1026
Bme18I GGWCC 1 cut(s) 740
BmeRI GACNNNNNGTC 1 cut(s) 154
BmeT110I CYCGRG 1 cut(s) 656
BmgBI CACGTC 1 cut(s) 124
BmgT120I GGNCC 2 cut(s) 256, 740
BmiI GGNNCC 2 cut(s) 643, 689
BmrFI CCNGG 3 cut(s) 744, 915, 1026
BmrI ACTGGG 1 cut(s) 754
BmsI GCATC 2 cut(s) 487, 870
BmtI GCTAGC 1 cut(s) 44
BmuI ACTGGG 1 cut(s) 754
BpiI GAAGAC 3 cut(s) 76, 109, 153
Bpu10I CCTNAGC 1 cut(s) 242
Bpu14I TTCGAA 2 cut(s) 90, 871
BpuEI CTTGAG 1 cut(s) 281
BsaBI GATNNNNATC 1 cut(s) 11
BsaHI GRCGYC 1 cut(s) 106
BsaJI CCNNGG 5 cut(s) 64, 297, 669, 856, 914
Bsc4I CCNNNNNNNGG 1 cut(s) 721
Bse1I ACTGG 2 cut(s) 760, 905
Bse3DI GCAATG 1 cut(s) 1012
Bse8I GATNNNNATC 1 cut(s) 11
BseBI CCWGG 3 cut(s) 744, 915, 1026
BseDI CCNNGG 5 cut(s) 64, 297, 669, 856, 914
BseGI GGATG 3 cut(s) 502, 813, 1102
BseJI GATNNNNATC 1 cut(s) 11
BseLI CCNNNNNNNGG 1 cut(s) 721
BseMI GCAATG 1 cut(s) 1012
BseMII CTCAG 1 cut(s) 615
BseNI ACTGG 2 cut(s) 760, 905
BseRI GAGGAG 1 cut(s) 674
BseXI GCAGC 5 cut(s) 280, 619, 721, 775, 778
Bsh1236I CGCG 2 cut(s) 119, 291
BshFI GGCC 3 cut(s) 257, 668, 1029
BshNI GGYRCC 1 cut(s) 687
BsiHKAI GWGCWC 1 cut(s) 652
BsiHKCI CYCGRG 1 cut(s) 656
BsiSI CCGG 2 cut(s) 134, 194
BslFI GGGAC 2 cut(s) 367, 596
BslI CCNNNNNNNGG 1 cut(s) 721
BsmAI GTCTC 1 cut(s) 79
BsmBI CGTCTC 1 cut(s) 79
BsmFI GGGAC 2 cut(s) 367, 596
BsmI GAATGC 3 cut(s) 227, 465, 939
BsnI GGCC 3 cut(s) 257, 668, 1029
BsoBI CYCGRG 1 cut(s) 656
Bsp119I TTCGAA 2 cut(s) 90, 871
Bsp1286I GDGCHC 1 cut(s) 652
Bsp143I GATC 4 cut(s) 6, 138, 190, 546
BspACI CCGC 6 cut(s) 78, 197, 691, 990, 1018, 1142
BspANI GGCC 3 cut(s) 257, 668, 1029
BspCNI CTCAG 1 cut(s) 614
BspFNI CGCG 2 cut(s) 119, 291
BspLI GGNNCC 2 cut(s) 643, 689
BspMI ACCTGC 1 cut(s) 598
BspOI GCTAGC 1 cut(s) 44
BspPI GGATC 2 cut(s) 185, 541
BspQI GCTCTTC 2 cut(s) 231, 809
BspT104I TTCGAA 2 cut(s) 90, 871
BspT107I GGYRCC 1 cut(s) 687
BsrDI GCAATG 1 cut(s) 1012
BsrI ACTGG 2 cut(s) 760, 905
BssECI CCNNGG 5 cut(s) 64, 297, 669, 856, 914
BssMI GATC 4 cut(s) 6, 138, 190, 546
BssNI GRCGYC 1 cut(s) 106
BssSI CACGAG 1 cut(s) 1155
BssT1I CCWWGG 4 cut(s) 64, 297, 669, 856
Bst2BI CACGAG 1 cut(s) 1155
Bst2UI CCWGG 3 cut(s) 744, 915, 1026
Bst4CI ACNGT 1 cut(s) 394
Bst6I CTCTTC 3 cut(s) 231, 809, 818
BstACI GRCGYC 1 cut(s) 106
BstBI TTCGAA 2 cut(s) 90, 871
BstC8I GCNNGC 1 cut(s) 42
BstDEI CTNAG 2 cut(s) 242, 601
BstF5I GGATG 3 cut(s) 502, 813, 1102
BstFNI CGCG 2 cut(s) 119, 291
BstHHI GCGC 2 cut(s) 121, 293
BstKTI GATC 4 cut(s) 9, 141, 193, 549
BstMAI GTCTC 1 cut(s) 79
BstMBI GATC 4 cut(s) 6, 138, 190, 546
BstMWI GCNNNNNNNGC 3 cut(s) 506, 958, 1026
BstNI CCWGG 3 cut(s) 744, 915, 1026
BstNSI RCATGY 2 cut(s) 177, 1170
BstSCI CCNGG 3 cut(s) 742, 913, 1024
BstSFI CTRYAG 1 cut(s) 393
BstUI CGCG 2 cut(s) 119, 291
BstV1I GCAGC 5 cut(s) 280, 619, 721, 775, 778
BstV2I GAAGAC 3 cut(s) 76, 109, 153
BstX2I RGATCY 1 cut(s) 546
BstYI RGATCY 1 cut(s) 546
BsuRI GGCC 3 cut(s) 257, 668, 1029
BtrI CACGTC 1 cut(s) 124
BtsCI GGATG 3 cut(s) 502, 813, 1102
BtsIMutI CAGTG 2 cut(s) 390, 767
BveI ACCTGC 1 cut(s) 598
Cac8I GCNNGC 1 cut(s) 42
CfoI GCGC 2 cut(s) 121, 293
Cfr13I GGNCC 2 cut(s) 256, 740
CseI GACGC 1 cut(s) 114
Csp6I GTAC 1 cut(s) 688
CviAII CATG 4 cut(s) 174, 431, 551, 1167
CviQI GTAC 1 cut(s) 688
DdeI CTNAG 2 cut(s) 242, 601
DpnI GATC 4 cut(s) 8, 140, 192, 548
DpnII GATC 4 cut(s) 6, 138, 190, 546
DriI GACNNNNNGTC 1 cut(s) 154
Eam1104I CTCTTC 3 cut(s) 231, 809, 818
Eam1105I GACNNNNNGTC 1 cut(s) 154
EarI CTCTTC 3 cut(s) 231, 809, 818
EciI GGCGGA 1 cut(s) 212
Eco130I CCWWGG 4 cut(s) 64, 297, 669, 856
Eco147I AGGCCT 1 cut(s) 668
Eco32I GATATC 2 cut(s) 622, 891
Eco47I GGWCC 1 cut(s) 740
Eco88I CYCGRG 1 cut(s) 656
EcoRI GAATTC 2 cut(s) 747, 873
EcoRII CCWGG 3 cut(s) 742, 913, 1024
EcoRV GATATC 2 cut(s) 622, 891
EcoT14I CCWWGG 4 cut(s) 64, 297, 669, 856
ErhI CCWWGG 4 cut(s) 64, 297, 669, 856
Esp3I CGTCTC 1 cut(s) 79
FaeI CATG 4 cut(s) 177, 434, 554, 1170
FalI AAGNNNNNCTT 4 cut(s) 447, 479, 914, 946
FaqI GGGAC 2 cut(s) 367, 596
FatI CATG 4 cut(s) 173, 430, 550, 1166
FauNDI CATATG 1 cut(s) 370
FblI GTMKAC 1 cut(s) 396
Fnu4HI GCNGC 6 cut(s) 294, 633, 735, 789, 792, 1018
FokI GGATG 3 cut(s) 509, 800, 1109
Fsp4HI GCNGC 6 cut(s) 294, 633, 735, 789, 792, 1018
FspBI CTAG 5 cut(s) 41, 65, 594, 783, 857
GlaI GCGC 2 cut(s) 120, 292
GluI GCNGC 6 cut(s) 294, 633, 735, 789, 792, 1018
HaeIII GGCC 3 cut(s) 257, 668, 1029
HapII CCGG 2 cut(s) 134, 194
HgaI GACGC 1 cut(s) 114
HhaI GCGC 2 cut(s) 121, 293
Hin1I GRCGYC 1 cut(s) 106
Hin1II CATG 4 cut(s) 177, 434, 554, 1170
Hin6I GCGC 2 cut(s) 119, 291
HinP1I GCGC 2 cut(s) 119, 291
HinfI GANTC 2 cut(s) 584, 1158
HpaII CCGG 2 cut(s) 134, 194
HphI GGTGA 3 cut(s) 488, 579, 937
Hpy166II GTNNAC 4 cut(s) 397, 780, 898, 928
Hpy188I TCNGA 7 cut(s) 452, 604, 619, 879, 907, 1008, 1067
Hpy188III TCNNGA 6 cut(s) 656, 700, 752, 1094, 1155, 1162
Hpy8I GTNNAC 4 cut(s) 397, 780, 898, 928
Hpy99I CGWCG 1 cut(s) 128
HpyAV CCTTC 3 cut(s) 189, 658, 700
HpyCH4III ACNGT 1 cut(s) 394
HpyCH4IV ACGT 1 cut(s) 123
HpyCH4V TGCA 6 cut(s) 227, 500, 554, 560, 635, 794
HpyF10VI GCNNNNNNNGC 3 cut(s) 506, 958, 1026
HpyF3I CTNAG 2 cut(s) 242, 601
HpySE526I ACGT 1 cut(s) 123
Hsp92I GRCGYC 1 cut(s) 106
Hsp92II CATG 4 cut(s) 177, 434, 554, 1170
HspAI GCGC 2 cut(s) 119, 291
KpnI GGTACC 1 cut(s) 691
Kzo9I GATC 4 cut(s) 6, 138, 190, 546
LguI GCTCTTC 2 cut(s) 231, 809
LmnI GCTCC 3 cut(s) 136, 506, 958
Lsp1109I GCAGC 5 cut(s) 280, 619, 721, 775, 778
LweI GCATC 2 cut(s) 487, 870
MaeI CTAG 5 cut(s) 41, 65, 594, 783, 857
MaeII ACGT 1 cut(s) 123
MaeIII GTNAC 4 cut(s) 169, 596, 1152, 1159
MalI GATC 4 cut(s) 8, 140, 192, 548
MboI GATC 4 cut(s) 6, 138, 190, 546
MboII GAAGA 8 cut(s) 76, 79, 114, 158, 248, 435, 796, 835
MfeI CAATTG 1 cut(s) 627
MflI RGATCY 1 cut(s) 546
MhlI GDGCHC 1 cut(s) 652
MlyI GAGTC 2 cut(s) 578, 1167
MmeI TCCRAC 2 cut(s) 307, 958
MroXI GAANNNNTTC 2 cut(s) 710, 803
MseI TTAA 5 cut(s) 573, 771, 935, 1101, 1121
MspA1I CMGCKG 2 cut(s) 80, 1144
MspI CCGG 2 cut(s) 134, 194
MspR9I CCNGG 3 cut(s) 744, 915, 1026
MunI CAATTG 1 cut(s) 627
Mva1269I GAATGC 3 cut(s) 227, 465, 939
MvaI CCWGG 3 cut(s) 744, 915, 1026
MvnI CGCG 2 cut(s) 119, 291
MwoI GCNNNNNNNGC 3 cut(s) 506, 958, 1026
NdeI CATATG 1 cut(s) 370
NdeII GATC 4 cut(s) 6, 138, 190, 546
NheI GCTAGC 1 cut(s) 40
NlaIII CATG 4 cut(s) 177, 434, 554, 1170
NlaIV GGNNCC 2 cut(s) 643, 689
NmuCI GTSAC 2 cut(s) 1152, 1159
NspI RCATGY 2 cut(s) 177, 1170
NspV TTCGAA 2 cut(s) 90, 871
PaeR7I CTCGAG 1 cut(s) 656
PaqCI CACCTGC 1 cut(s) 598
PceI AGGCCT 1 cut(s) 668
PciSI GCTCTTC 2 cut(s) 231, 809
PcsI WCGNNNNNNNCGW 1 cut(s) 123
PctI GAATGC 3 cut(s) 227, 465, 939
PdmI GAANNNNTTC 2 cut(s) 710, 803
PflMI CCANNNNNTGG 1 cut(s) 721
PfoI TCCNGGA 1 cut(s) 742
PkrI GCNGC 6 cut(s) 295, 634, 736, 790, 793, 1019
PleI GAGTC 2 cut(s) 578, 1166
PpsI GAGTC 2 cut(s) 578, 1166
Psp6I CCWGG 3 cut(s) 742, 913, 1024
PspGI CCWGG 3 cut(s) 742, 913, 1024
PspN4I GGNNCC 2 cut(s) 643, 689
PspPI GGNCC 2 cut(s) 256, 740
PsuI RGATCY 1 cut(s) 546
RsaI GTAC 1 cut(s) 689
RsaNI GTAC 1 cut(s) 688
SapI GCTCTTC 2 cut(s) 231, 809
SaqAI TTAA 5 cut(s) 573, 771, 935, 1101, 1121
SatI GCNGC 6 cut(s) 294, 633, 735, 789, 792, 1018
Sau3AI GATC 4 cut(s) 6, 138, 190, 546
Sau96I GGNCC 2 cut(s) 256, 740
SchI GAGTC 2 cut(s) 578, 1167
ScrFI CCNGG 3 cut(s) 744, 915, 1026
SduI GDGCHC 1 cut(s) 652
SfaNI GCATC 2 cut(s) 487, 870
SfcI CTRYAG 1 cut(s) 393
Sfr274I CTCGAG 1 cut(s) 656
SfuI TTCGAA 2 cut(s) 90, 871
SinI GGWCC 1 cut(s) 740
SlaI CTCGAG 1 cut(s) 656
SmlI CTYRAG 2 cut(s) 260, 656
SmoI CTYRAG 2 cut(s) 260, 656
SseBI AGGCCT 1 cut(s) 668
SsiI CCGC 6 cut(s) 78, 197, 691, 990, 1018, 1142
SspMI CTAG 5 cut(s) 41, 65, 594, 783, 857
StuI AGGCCT 1 cut(s) 668
StyD4I CCNGG 3 cut(s) 742, 913, 1024
StyI CCWWGG 4 cut(s) 64, 297, 669, 856
TaaI ACNGT 1 cut(s) 394
TaiI ACGT 1 cut(s) 126
TaqI TCGA 6 cut(s) 90, 126, 158, 182, 657, 871
TauI GCSGC 1 cut(s) 1020
Tru1I TTAA 5 cut(s) 573, 771, 935, 1101, 1121
Tru9I TTAA 5 cut(s) 573, 771, 935, 1101, 1121
TscAI CASTG 2 cut(s) 397, 767
TseFI GTSAC 2 cut(s) 1152, 1159
TseI GCWGC 5 cut(s) 293, 632, 734, 788, 791
Tsp45I GTSAC 2 cut(s) 1152, 1159
TspDTI ATGAA 5 cut(s) 249, 287, 387, 455, 719
TspGWI ACGGA 1 cut(s) 806
TspRI CASTG 2 cut(s) 397, 767
Van91I CCANNNNNTGG 1 cut(s) 721
VpaK11BI GGWCC 1 cut(s) 740
XapI RAATTY 5 cut(s) 374, 569, 747, 873, 909
XceI RCATGY 2 cut(s) 177, 1170
XcmI CCANNNNNNNNNTGG 1 cut(s) 951
XhoI CTCGAG 1 cut(s) 656
XmaJI CCTAGG 2 cut(s) 64, 856
XmiI GTMKAC 1 cut(s) 396
XmnI GAANNNNTTC 2 cut(s) 710, 803
XspI CTAG 5 cut(s) 41, 65, 594, 783, 857
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.