Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0005544

Physical Location & Seq

Genomic Coordinates Reverse (-)

14583185 .. 14588651

Gene Structure 5467 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00294510.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2040 bp

ATGGCATTAAATAAGTCTGGAAGTGATCGCCGTTGTCCATGGCTGATTCTCCCTGCAACTCTTCTCATTGTCCTCCTTGCCTATGGAGCTCATGCTGAGGACATTTTTCTTCAATGGGACGTCGCCGTCGATACGAGTATCAGACCTGCATCCGTGGACCAACCGGTGATTACAATCAATGGGAGGTTTCCAGGGCCGCTTATCAACGCCACAACCAATGATATTGTTCATGTTAATGTGTTTAATCATATGGATGAAGAGCTGCTCATAACATGGAATGGGATACAACAAAGGCTAAACTCTTGGCAAGATGGAGTTTCCGGAACTAATTGTCCCATTCAGCCTTTTACAAACTGGACGTATGTGTTCCAACTCAAGGATCAAATTGGTACTTTTTCCTACTTTCCCTCCATCAACTTCCTCAAAGCTGGTGGGGGATTCGGTCCGATTCAGATCCATAATCGTCCTGTCATCCAAGTTCCATATCTGAAACCGGAAGCCGAGTTCGATCTTCTCATTGGTGATTGGTATGAGACGAGTTACAAGGATCTTAGGTCCATGCTCAGCAGCATGCAAATGGCCGCCTATAATAGTACCCCTGATAAAATCTTGATGAATGGAAAAGGATCATACGGGGATCCTCTAGCAAAGGCATATGAGTCCTTCACTGTTACACCAGGGAAGACATACCTAATTAGGATATCAAATGTTGGAAGTGCGTGGAGTTTGAATTTCTTGATTCAAAATCATCGAATGGTGCTGGTTGAAACAGAAGGATCCTATACTAATGAAATCACCTTGGATTCTCTTGATGTACATGTTGGACAGTCCTACGCTGTGCTTGTCACAGCTGATCAAGCCACTTCAGACTATTACATGGTAGCAACTCCTAAATTGGTTAAAATCACAGATATGACCAAATTTGGAATTGGTGTGCTGCACTATGATAGCTCCACCACAGCTGCTGGTGGACCTCTACCAATTGGCCCTGAACCTTTTGATATAGAACTATCCATCAATCAAGCCAACTCCATCAGGTGGAACCTGACTACAGGGGCAGCAAGGCCTAATCCACAAGGATCCTTCAACGTTCATAACGTGACACTATCACAGACATTCATACTAAGTGCATCAACAGCAGAGATTGATGGTGCACCCAAGTTTACTGTCAACAATGTGTCCTACTTAACCCCAGATACACCATTGAAGTTGGCTGATCACTTGGTCAACGGTTCTGGAGTTTACGAGCTTGATGAATTCTCTACTAACTCTACAAATGTTAAACCTGAGCGTGGAGTTTTTGTTGCAAGTGGGATACACAAAGGGTGGATAGAACTTGTTTTCCACAATGATTTGGAGGTCATGGACTCTTGGCATTTGGATGGCTTCGGATTCTTTGTTGTTGGCTACGGCCGTGGAAAGTGGAGTGCTAAGTCGCGCTCAACCTACAACCTTTATGATCCCGTGGTGCGTTCTACTGTACAAGTGTACCCACGGAGGTGGAGTGCAGTTTATGCATACCTTGACAACATTGGAATGTGGAATTTGAGGTCACAGCACTTGAAAAATTGGTATTTGGGTGAAGAGCTATATGTGAGAGTCTATGATGCTGATCCCAACCCTGCAAAAGAGAGGCCTTCCCCTAATAACATTCTTCTTTGTGGAAAATTTGCCCCGCCCACTCCTCCTTCTGGACCGTCTGCACCTCCTCCAAAGCCATCTAGTGCACCATCTTTGCAAATTACAGCAGCAAGGTCTCATATTGCCATTATCTGCATTATTGCAACATTCATTCAAATATCCAGAGTCTCTGAATCCACCAGTTCGAGTTCTCTTCCTCATTTTGATCGCCTGAAAAGCCTCACCTTTCCATCCAATGATTATAGCATAAGGGAAGTGATTCAAAGTTCAAATATTTCGTTCTTGCTGAATTCACGGTTACTGATTTTAAAGTTCAGTGTTGACCATGATATTGATATCGTCCATTTTGAACTTTCAAAGAAACTTTCTTTCTCAGTGTTTGGTTTTCTGGAACTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

679

Amino Acids

75.43

Weight (kDa)

5.87

Isoelectric Point (pI)

40.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cu-oxidase_3	PF07732	48 - 155	7.5e-36	Multicopper oxidase
Cu-oxidase	PF00394	171 - 317	5.7e-39	Multicopper oxidase
Cu-oxidase_2	PF07731	400 - 537	6.3e-27	Multicopper oxidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005544)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g18341 FvH4_7g18350
malus_domestica	MD01G1098800.v1.1 MD01G1099000.v1.1 MD07G1165500.v1.1 MD07G1165800.v1.1
prunus_persica	Prupe.2G205500_v2.0.a1 Prupe.2G205600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0362061 RchiOBHm_Chr1g0362091 RchiOBHm_Chr7g0206161
rosa_laevigata	RLG00000027672 RLG00000027676
rosa_multiflora	Rmu_co8403161.1_g000001 Rmu_sc0010614.1_g000004
rosa_roxburghii	Rroxscaffold_4G00294510 Rroxscaffold_4G00294540
rosa_rugosa	Rorug01G0293200 Rorug01G0293600
rosa_samantha	Rh1BG266700 Rh1BG267000 Rh1CG284800 Rh1CG285100 Rh1DG296700 Rh1DG297100 Rh7DG233000
rosa_wichuraiana	Rw1G027020 Rw1G027050 Rw7G019480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	125
AatII	GACGTC	1 cut(s)	123
Acc36I	ACCTGC	1 cut(s)	154
AccB7I	CCANNNNNTGG	1 cut(s)	1038
AccII	CGCG	1 cut(s)	1438
AccIII	TCCGGA	1 cut(s)	320
AciI	CCGC	3 cut(s)	197, 582, 1676
AclI	AACGTT	1 cut(s)	1089
AcoI	YGGCCR	2 cut(s)	579, 1411
AcsI	RAATTY	6 cut(s)	730, 920, 1256, 1543, 1667, 1930
AcuI	CTGAAG	1 cut(s)	849
AcyI	GRCGYC	1 cut(s)	120
AfaI	GTAC	5 cut(s)	391, 595, 816, 1482, 1490
AfiI	CCNNNNNNNGG	4 cut(s)	376, 1038, 1292, 1691
AflIII	ACRYGT	1 cut(s)	817
AgeI	ACCGGT	1 cut(s)	163
AjnI	CCWGG	2 cut(s)	190, 676
AleI	CACNNNNGTG	1 cut(s)	1498
AloI	GAACNNNNNNTCC	6 cut(s)	316, 348, 488, 520, 1326, 1358
AluBI	AGCT	8 cut(s)	89, 262, 428, 851, 951, 962, 1249, 1588
AluI	AGCT	8 cut(s)	89, 262, 428, 851, 951, 962, 1249, 1588
Alw21I	GWGCWC	3 cut(s)	91, 1156, 1729
Alw26I	GTCTC	3 cut(s)	527, 1761, 1813
Alw44I	GTGCAC	2 cut(s)	1152, 1725
AlwNI	CAGNNNCTG	1 cut(s)	965
Aor13HI	TCCGGA	1 cut(s)	320
AoxI	GGCC	6 cut(s)	194, 579, 985, 1064, 1411, 1634
ApaLI	GTGCAC	2 cut(s)	1152, 1725
ApeKI	GCWGC	6 cut(s)	262, 567, 937, 962, 1058, 1748
ApoI	RAATTY	6 cut(s)	730, 920, 1256, 1543, 1667, 1930
AsiGI	ACCGGT	1 cut(s)	163
Asp700I	GAANNNNTTC	1 cut(s)	1899
AspLEI	GCGC	1 cut(s)	1440
AspS9I	GGNCC	7 cut(s)	157, 194, 443, 555, 971, 986, 1694
AsuHPI	GGTGA	5 cut(s)	178, 533, 787, 1592, 1855
AvaII	GGWCC	5 cut(s)	157, 443, 555, 971, 1694
BaeGI	GKGCMC	2 cut(s)	1156, 1729
BaeI	ACNNNNGTAYC	2 cut(s)	1472, 1505
BamHI	GGATCC	3 cut(s)	637, 776, 1079
BanII	GRGCYC	1 cut(s)	91
BbsI	GAAGAC	1 cut(s)	689
Bbv12I	GWGCWC	3 cut(s)	91, 1156, 1729
BbvCI	CCTCAGC	1 cut(s)	96
BbvI	GCAGC	6 cut(s)	249, 579, 924, 949, 1070, 1760
BccI	CCATC	9 cut(s)	305, 419, 1022, 1040, 1142, 1376, 1726, 1738, 1879
BceAI	ACGGC	4 cut(s)	15, 110, 1398, 1426
BciT130I	CCWGG	2 cut(s)	192, 678
BciVI	GTATCC	2 cut(s)	276, 1308
BclI	TGATCA	2 cut(s)	853, 1216
BcoDI	GTCTC	3 cut(s)	527, 1761, 1813
BfaI	CTAG	2 cut(s)	644, 1722
BfmI	CTRYAG	1 cut(s)	1050
BfuAI	ACCTGC	1 cut(s)	154
BfuI	GTATCC	2 cut(s)	276, 1308
BisI	GCNGC	8 cut(s)	197, 263, 568, 582, 938, 963, 1059, 1749
BlpI	GCTNAGC	1 cut(s)	563
BlsI	GCNGC	8 cut(s)	198, 264, 569, 583, 939, 964, 1060, 1750
Bme1390I	CCNGG	2 cut(s)	192, 678
Bme18I	GGWCC	5 cut(s)	157, 443, 555, 971, 1694
BmgT120I	GGNCC	7 cut(s)	157, 194, 443, 555, 971, 986, 1694
BmiI	GGNNCC	4 cut(s)	639, 778, 1043, 1081
BmrFI	CCNGG	2 cut(s)	192, 678
BmsI	GCATC	3 cut(s)	158, 1139, 1597
BpiI	GAAGAC	1 cut(s)	689
BpmI	CTGGAG	1 cut(s)	1257
Bpu10I	CCTNAGC	2 cut(s)	96, 1287
Bpu1102I	GCTNAGC	1 cut(s)	563
BpuEI	CTTGAG	1 cut(s)	359
BsaBI	GATNNNNATC	3 cut(s)	173, 452, 1611
BsaHI	GRCGYC	1 cut(s)	120
BsaI	GGTCTC	1 cut(s)	1761
BsaJI	CCNNGG	8 cut(s)	38, 153, 191, 677, 798, 1414, 1464, 1493
BsaWI	WCCGGW	3 cut(s)	163, 320, 493
BsaXI	ACNNNNNCTCC	6 cut(s)	392, 422, 1287, 1317, 1489, 1519
Bsc4I	CCNNNNNNNGG	4 cut(s)	376, 1038, 1292, 1691
Bse118I	RCCGGY	1 cut(s)	163
Bse1I	ACTGG	2 cut(s)	359, 1821
Bse8I	GATNNNNATC	3 cut(s)	173, 452, 1611
BseAI	TCCGGA	1 cut(s)	320
BseBI	CCWGG	2 cut(s)	192, 678
BseDI	CCNNGG	8 cut(s)	38, 153, 191, 677, 798, 1414, 1464, 1493
BseGI	GGATG	5 cut(s)	149, 259, 471, 1387, 1871
BseJI	GATNNNNATC	3 cut(s)	173, 452, 1611
BseLI	CCNNNNNNNGG	4 cut(s)	376, 1038, 1292, 1691
BseMII	CTCAG	4 cut(s)	87, 577, 1278, 2028
BseNI	ACTGG	2 cut(s)	359, 1821
BseRI	GAGGAG	2 cut(s)	1674, 1698
BseSI	GKGCMC	2 cut(s)	1156, 1729
BseX3I	CGGCCG	1 cut(s)	1411
BseXI	GCAGC	6 cut(s)	249, 579, 924, 949, 1070, 1760
BsgI	GTGCAG	3 cut(s)	923, 1527, 1686
Bsh1236I	CGCG	1 cut(s)	1438
Bsh1285I	CGRYCG	1 cut(s)	1414
BshFI	GGCC	6 cut(s)	196, 581, 987, 1066, 1413, 1636
BshTI	ACCGGT	1 cut(s)	163
BsiEI	CGRYCG	1 cut(s)	1414
BsiHKAI	GWGCWC	3 cut(s)	91, 1156, 1729
BsiSI	CCGG	3 cut(s)	164, 321, 494
BslFI	GGGAC	2 cut(s)	131, 318
BslI	CCNNNNNNNGG	4 cut(s)	376, 1038, 1292, 1691
BsmAI	GTCTC	3 cut(s)	527, 1761, 1813
BsmBI	CGTCTC	1 cut(s)	527
BsmFI	GGGAC	2 cut(s)	131, 318
BsnI	GGCC	6 cut(s)	196, 581, 987, 1066, 1413, 1636
Bso31I	GGTCTC	1 cut(s)	1761
Bsp1286I	GDGCHC	3 cut(s)	91, 1156, 1729
Bsp13I	TCCGGA	1 cut(s)	320
Bsp1407I	TGTACA	2 cut(s)	814, 1480
Bsp1720I	GCTNAGC	1 cut(s)	563
Bsp19I	CCATGG	1 cut(s)	38
BspACI	CCGC	3 cut(s)	197, 582, 1676
BspANI	GGCC	6 cut(s)	196, 581, 987, 1066, 1413, 1636
BspCNI	CTCAG	4 cut(s)	88, 576, 1279, 2027
BspEI	TCCGGA	1 cut(s)	320
BspFNI	CGCG	1 cut(s)	1438
BspLI	GGNNCC	4 cut(s)	639, 778, 1043, 1081
BspMI	ACCTGC	1 cut(s)	154
BspQI	GCTCTTC	2 cut(s)	252, 1578
BspTNI	GGTCTC	1 cut(s)	1761
BsrFI	RCCGGY	1 cut(s)	163
BsrGI	TGTACA	2 cut(s)	814, 1480
BsrI	ACTGG	2 cut(s)	359, 1821
BssAI	RCCGGY	1 cut(s)	163
BssECI	CCNNGG	8 cut(s)	38, 153, 191, 677, 798, 1414, 1464, 1493
BssNI	GRCGYC	1 cut(s)	120
BssT1I	CCWWGG	2 cut(s)	38, 798
Bst2UI	CCWGG	2 cut(s)	192, 678
Bst4CI	ACNGT	7 cut(s)	670, 828, 1168, 1232, 1480, 1698, 1938
Bst6I	CTCTTC	4 cut(s)	66, 252, 1578, 1839
BstACI	GRCGYC	1 cut(s)	120
BstAPI	GCANNNNNTGC	1 cut(s)	1514
BstAUI	TGTACA	2 cut(s)	814, 1480
BstC8I	GCNNGC	1 cut(s)	572
BstDEI	CTNAG	7 cut(s)	96, 551, 563, 1124, 1287, 1431, 2014
BstDSI	CCRYGG	5 cut(s)	38, 153, 1414, 1464, 1493
BstF5I	GGATG	5 cut(s)	149, 259, 471, 1387, 1871
BstFNI	CGCG	1 cut(s)	1438
BstHHI	GCGC	1 cut(s)	1440
BstMAI	GTCTC	3 cut(s)	527, 1761, 1813
BstMCI	CGRYCG	1 cut(s)	1414
BstMWI	GCNNNNNNNGC	5 cut(s)	86, 857, 1136, 1514, 1857
BstNI	CCWGG	2 cut(s)	192, 678
BstNSI	RCATGY	2 cut(s)	574, 821
BstSCI	CCNGG	2 cut(s)	190, 676
BstSFI	CTRYAG	1 cut(s)	1050
BstSLI	GKGCMC	2 cut(s)	1156, 1729
BstUI	CGCG	1 cut(s)	1438
BstV1I	GCAGC	6 cut(s)	249, 579, 924, 949, 1070, 1760
BstV2I	GAAGAC	1 cut(s)	689
BstX2I	RGATCY	5 cut(s)	453, 547, 637, 776, 1079
BstXI	CCANNNNNNTGG	1 cut(s)	1500
BstYI	RGATCY	5 cut(s)	453, 547, 637, 776, 1079
BstZI	CGGCCG	1 cut(s)	1411
BsuI	GTATCC	2 cut(s)	276, 1308
BsuRI	GGCC	6 cut(s)	196, 581, 987, 1066, 1413, 1636
BtgI	CCRYGG	5 cut(s)	38, 153, 1414, 1464, 1493
BtsCI	GGATG	5 cut(s)	149, 259, 471, 1387, 1871
BtsIMutI	CAGTG	3 cut(s)	666, 1963, 2022
BveI	ACCTGC	1 cut(s)	154
Cac8I	GCNNGC	1 cut(s)	572
CaiI	CAGNNNCTG	1 cut(s)	965
CfoI	GCGC	1 cut(s)	1440
Cfr10I	RCCGGY	1 cut(s)	163
Cfr13I	GGNCC	7 cut(s)	157, 194, 443, 555, 971, 986, 1694
CpoI	CGGWCCG	1 cut(s)	443
Csp6I	GTAC	5 cut(s)	390, 594, 815, 1481, 1489
CspAI	ACCGGT	1 cut(s)	163
CspCI	CAANNNNNGTGG	2 cut(s)	412, 447
CspI	CGGWCCG	1 cut(s)	443
CviQI	GTAC	5 cut(s)	390, 594, 815, 1481, 1489
DdeI	CTNAG	7 cut(s)	96, 551, 563, 1124, 1287, 1431, 2014
DraI	TTTAAA	1 cut(s)	1950
DrdI	GACNNNNNNGTC	1 cut(s)	125
DseDI	GACNNNNNNGTC	1 cut(s)	125
EaeI	YGGCCR	2 cut(s)	579, 1411
EagI	CGGCCG	1 cut(s)	1411
Eam1104I	CTCTTC	4 cut(s)	66, 252, 1578, 1839
EarI	CTCTTC	4 cut(s)	66, 252, 1578, 1839
Ecl136II	GAGCTC	1 cut(s)	89
EclXI	CGGCCG	1 cut(s)	1411
Eco130I	CCWWGG	2 cut(s)	38, 798
Eco147I	AGGCCT	2 cut(s)	1066, 1636
Eco24I	GRGCYC	1 cut(s)	91
Eco31I	GGTCTC	1 cut(s)	1761
Eco32I	GATATC	2 cut(s)	702, 1978
Eco47I	GGWCC	5 cut(s)	157, 443, 555, 971, 1694
Eco52I	CGGCCG	1 cut(s)	1411
Eco53kI	GAGCTC	1 cut(s)	89
Eco57I	CTGAAG	1 cut(s)	849
EcoICRI	GAGCTC	1 cut(s)	89
EcoRI	GAATTC	2 cut(s)	1256, 1930
EcoRII	CCWGG	2 cut(s)	190, 676
EcoRV	GATATC	2 cut(s)	702, 1978
EcoT14I	CCWWGG	2 cut(s)	38, 798
EcoT22I	ATGCAT	1 cut(s)	1519
EcoT38I	GRGCYC	1 cut(s)	91
ErhI	CCWWGG	2 cut(s)	38, 798
Esp3I	CGTCTC	1 cut(s)	527
FaqI	GGGAC	2 cut(s)	131, 318
FauI	CCCGC	1 cut(s)	1683
FauNDI	CATATG	2 cut(s)	249, 655
FbaI	TGATCA	2 cut(s)	853, 1216
Fnu4HI	GCNGC	8 cut(s)	197, 263, 568, 582, 938, 963, 1059, 1749
FokI	GGATG	5 cut(s)	136, 266, 458, 1394, 1858
FriOI	GRGCYC	1 cut(s)	91
Fsp4HI	GCNGC	8 cut(s)	197, 263, 568, 582, 938, 963, 1059, 1749
FspBI	CTAG	2 cut(s)	644, 1722
GlaI	GCGC	1 cut(s)	1439
GluI	GCNGC	8 cut(s)	197, 263, 568, 582, 938, 963, 1059, 1749
GsuI	CTGGAG	1 cut(s)	1257
HaeIII	GGCC	6 cut(s)	196, 581, 987, 1066, 1413, 1636
HapII	CCGG	3 cut(s)	164, 321, 494
HhaI	GCGC	1 cut(s)	1440
Hin1I	GRCGYC	1 cut(s)	120
Hin6I	GCGC	1 cut(s)	1438
HinP1I	GCGC	1 cut(s)	1438
HincII	GTYRAC	3 cut(s)	1171, 1228, 1963
HindII	GTYRAC	3 cut(s)	1171, 1228, 1963
HpaII	CCGG	3 cut(s)	164, 321, 494
HphI	GGTGA	5 cut(s)	178, 533, 787, 1592, 1855
Hpy188I	TCNGA	7 cut(s)	143, 447, 453, 489, 868, 1391, 1813
Hpy188III	TCNNGA	9 cut(s)	18, 321, 610, 736, 809, 1236, 1692, 1803, 2030
Hpy99I	CGWCG	2 cut(s)	125, 131
HpyAV	CCTTC	5 cut(s)	673, 767, 1093, 1647, 1698
HpyCH4III	ACNGT	7 cut(s)	670, 828, 1168, 1232, 1480, 1698, 1938
HpyCH4IV	ACGT	4 cut(s)	120, 359, 1089, 1098
HpyF10VI	GCNNNNNNNGC	5 cut(s)	86, 857, 1136, 1514, 1857
HpyF3I	CTNAG	7 cut(s)	96, 551, 563, 1124, 1287, 1431, 2014
HpySE526I	ACGT	4 cut(s)	120, 359, 1089, 1098
Hsp92I	GRCGYC	1 cut(s)	120
HspAI	GCGC	1 cut(s)	1438
Kpn2I	TCCGGA	1 cut(s)	320
Ksp22I	TGATCA	2 cut(s)	853, 1216
LguI	GCTCTTC	2 cut(s)	252, 1578
LmnI	GCTCC	2 cut(s)	86, 956
Lsp1109I	GCAGC	6 cut(s)	249, 579, 924, 949, 1070, 1760
LweI	GCATC	3 cut(s)	158, 1139, 1597
MaeI	CTAG	2 cut(s)	644, 1722
MaeII	ACGT	4 cut(s)	120, 359, 1089, 1098
MaeIII	GTNAC	6 cut(s)	539, 670, 844, 1099, 1551, 1938
MboII	GAAGA	8 cut(s)	53, 101, 269, 503, 694, 1595, 1646, 1826
MfeI	CAATTG	1 cut(s)	981
MflI	RGATCY	5 cut(s)	453, 547, 637, 776, 1079
MhlI	GDGCHC	3 cut(s)	91, 1156, 1729
MlyI	GAGTC	4 cut(s)	668, 1361, 1608, 1815
MmeI	TCCRAC	3 cut(s)	394, 691, 802
Mph1103I	ATGCAT	1 cut(s)	1519
MroI	TCCGGA	1 cut(s)	320
MroXI	GAANNNNTTC	1 cut(s)	1899
MseI	TTAA	8 cut(s)	8, 234, 243, 900, 1187, 1281, 1949, 2038
MslI	CAYNNNNRTG	7 cut(s)	234, 252, 575, 911, 1380, 1498, 1535
MspA1I	CMGCKG	2 cut(s)	851, 962
MspI	CCGG	3 cut(s)	164, 321, 494
MspR9I	CCNGG	2 cut(s)	192, 678
MunI	CAATTG	1 cut(s)	981
MvaI	CCWGG	2 cut(s)	192, 678
MvnI	CGCG	1 cut(s)	1438
MwoI	GCNNNNNNNGC	5 cut(s)	86, 857, 1136, 1514, 1857
NcoI	CCATGG	1 cut(s)	38
NdeI	CATATG	2 cut(s)	249, 655
NlaIV	GGNNCC	4 cut(s)	639, 778, 1043, 1081
NmeAIII	GCCGAG	1 cut(s)	526
NmuCI	GTSAC	3 cut(s)	844, 1099, 1551
NsiI	ATGCAT	1 cut(s)	1519
NspI	RCATGY	2 cut(s)	574, 821
OliI	CACNNNNGTG	1 cut(s)	1498
PaeI	GCATGC	1 cut(s)	574
PceI	AGGCCT	2 cut(s)	1066, 1636
PciI	ACATGT	1 cut(s)	817
PciSI	GCTCTTC	2 cut(s)	252, 1578
PcsI	WCGNNNNNNNCGW	2 cut(s)	126, 1095
PdmI	GAANNNNTTC	1 cut(s)	1899
PfeI	GAWTC	8 cut(s)	46, 438, 448, 739, 803, 1392, 1814, 1900
PflMI	CCANNNNNTGG	1 cut(s)	1038
PinAI	ACCGGT	1 cut(s)	163
PkrI	GCNGC	8 cut(s)	198, 264, 569, 583, 939, 964, 1060, 1750
PleI	GAGTC	4 cut(s)	667, 1361, 1607, 1814
PpsI	GAGTC	4 cut(s)	667, 1361, 1607, 1814
PscI	ACATGT	1 cut(s)	817
Psp124BI	GAGCTC	1 cut(s)	91
Psp1406I	AACGTT	1 cut(s)	1089
Psp6I	CCWGG	2 cut(s)	190, 676
PspGI	CCWGG	2 cut(s)	190, 676
PspN4I	GGNNCC	4 cut(s)	639, 778, 1043, 1081
PspPI	GGNCC	7 cut(s)	157, 194, 443, 555, 971, 986, 1694
PstNI	CAGNNNCTG	1 cut(s)	965
PsuI	RGATCY	5 cut(s)	453, 547, 637, 776, 1079
PvuII	CAGCTG	2 cut(s)	851, 962
RsaI	GTAC	5 cut(s)	391, 595, 816, 1482, 1490
RsaNI	GTAC	5 cut(s)	390, 594, 815, 1481, 1489
RseI	CAYNNNNRTG	7 cut(s)	234, 252, 575, 911, 1380, 1498, 1535
Rsr2I	CGGWCCG	1 cut(s)	443
RsrII	CGGWCCG	1 cut(s)	443
SacI	GAGCTC	1 cut(s)	91
SapI	GCTCTTC	2 cut(s)	252, 1578
SaqAI	TTAA	8 cut(s)	8, 234, 243, 900, 1187, 1281, 1949, 2038
SatI	GCNGC	8 cut(s)	197, 263, 568, 582, 938, 963, 1059, 1749
Sau96I	GGNCC	7 cut(s)	157, 194, 443, 555, 971, 986, 1694
SchI	GAGTC	4 cut(s)	668, 1361, 1608, 1815
ScrFI	CCNGG	2 cut(s)	192, 678
SduI	GDGCHC	3 cut(s)	91, 1156, 1729
SfaNI	GCATC	3 cut(s)	158, 1139, 1597
SfcI	CTRYAG	1 cut(s)	1050
SinI	GGWCC	5 cut(s)	157, 443, 555, 971, 1694
SmiMI	CAYNNNNRTG	7 cut(s)	234, 252, 575, 911, 1380, 1498, 1535
SmlI	CTYRAG	1 cut(s)	374
SmoI	CTYRAG	1 cut(s)	374
SphI	GCATGC	1 cut(s)	574
SseBI	AGGCCT	2 cut(s)	1066, 1636
SsiI	CCGC	3 cut(s)	197, 582, 1676
SspI	AATATT	1 cut(s)	1915
SspMI	CTAG	2 cut(s)	644, 1722
SstI	GAGCTC	1 cut(s)	91
StuI	AGGCCT	2 cut(s)	1066, 1636
StyD4I	CCNGG	2 cut(s)	190, 676
StyI	CCWWGG	2 cut(s)	38, 798
TaaI	ACNGT	7 cut(s)	670, 828, 1168, 1232, 1480, 1698, 1938
TaiI	ACGT	4 cut(s)	123, 362, 1092, 1101
TaqI	TCGA	4 cut(s)	129, 507, 751, 1826
TaqII	GACCGA	1 cut(s)	431
TatI	WGTACW	2 cut(s)	814, 1480
TauI	GCSGC	2 cut(s)	199, 584
TfiI	GAWTC	8 cut(s)	46, 438, 448, 739, 803, 1392, 1814, 1900
Tru1I	TTAA	8 cut(s)	8, 234, 243, 900, 1187, 1281, 1949, 2038
Tru9I	TTAA	8 cut(s)	8, 234, 243, 900, 1187, 1281, 1949, 2038
TscAI	CASTG	3 cut(s)	673, 1963, 2022
TseFI	GTSAC	3 cut(s)	844, 1099, 1551
TseI	GCWGC	6 cut(s)	262, 567, 937, 962, 1058, 1748
Tsp45I	GTSAC	3 cut(s)	844, 1099, 1551
TspDTI	ATGAA	8 cut(s)	218, 270, 629, 804, 1082, 1108, 1269, 1780
TspGWI	ACGGA	2 cut(s)	142, 1510
TspRI	CASTG	3 cut(s)	673, 1963, 2022
Van91I	CCANNNNNTGG	1 cut(s)	1038
VneI	GTGCAC	2 cut(s)	1152, 1725
VpaK11BI	GGWCC	5 cut(s)	157, 443, 555, 971, 1694
XapI	RAATTY	6 cut(s)	730, 920, 1256, 1543, 1667, 1930
XceI	RCATGY	2 cut(s)	574, 821
XmnI	GAANNNNTTC	1 cut(s)	1899
XspI	CTAG	2 cut(s)	644, 1722
ZraI	GACGTC	1 cut(s)	121
Zsp2I	ATGCAT	1 cut(s)	1519

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.