Rroxscaffold_4G00316980

Glucomannan 4-beta-mannosyltransferase 9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0005977
Physical Location & Seq
Forward (+)
41935668 .. 41941196
5529 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00316980.1

Sequence Viewer

Length: 1539 bp
ATGGACGCGCTCGGGCTGGTTTTGCAGCTGGCGAAAGCACCACTGATCGTGCCGCTGTTGCGAGTTTTGGTGGCGGTGTGCTTGGGGATGTCTGTGTTGCTCTTTGTGGAGAAGGTGTACATGGGAATTGTCATAATGTTGGTCAAGGTTTTCAGAAGAAGACCTGAGAAGCGTTGGAAATGGGAGGCAATTAAGGATGATGTGGAGCTTGGCAATTCTGCTTACCCTATGGTTCTTGTGCAAATTCCAATGTACAATGAAAAAGAGGTATATCAACTTTCTATTGGCGCTGCATGTGGGCTTTCATGGCCTTCTGATAGGATCGTAGTTCAAGTTCTTGATGATTCAACTGACCCACTCATTAAGGATTTGGTGGAAGTAGAAGTGCAGAGATGGGCAAGCAAAGGAATAAACATAAAATACGAGGTCAGAGACAACAGAAAAGGGTACAAAGCAGGGGCTCTCAAAGAGGGAATGAAGCACTCTTACGCCAAACAGTGTGACTACGTCGCCATTTTTGATGCCGACTTCCAACCTGAGCCCGATTTTCTGTGGCGCACAATCCCATTTCTCCTCCACAACCCTGACATTGCTCTTGTCCAAGCTCGCTGGAAATATGTAAATGCTGATGAGTGCCTGATGACCAGAATGCAAGAGATGTCTCTAGATTACCATTTCACAGTGGAGCAGGAAGTTGGGTCTGCGACTCATGCCTTCTTTGGTTTCAATGGCACAGCTGGAGTGTGGAGAATCAGGGCCCTCAATGAGGCTGGAGGATGGAAGGACCGCACAACAGTGGAGGATATGGATTTAGCTGTACGAGCTAGTCTTAGAGGCTGGAAGTTCGTCTATATTTCTGACCTCAAGGTGAAAAATGAATTGCCCAGTACTTTCAAAGCCTACCGTTATCAGCAGCACCGATGGTCTTGTGGTCCGGCTAATCTCTTCCGGAAGATGGTAATGGAGATTTTAAGAAACAAGAAGGTGTCCCCATTGAAGAAGTTTCATGTGATCTACAGCTTCTTCTTTGTTCGCAAAATCGTAGCTCATATTGTCACCTTTGTCTTCTACTGTGTTATTTTACCAACAACGGTTTTGGTTCCTGAAGTTCAAGTTCCCATTTGGGGTGCTGTATACATTCCTTCTACCATTACTCTCCTCAATGCAGTTGGAACTCCTAGATCACTCCACCTATTGATTTTCTGGATTCTCTTTGAGAATGTAATGTCCCTGCACCGGACTAAGGCAACATTTATAGGCCTGTTTGAAGCAGGGAGAGTAAATGAATGGGTCGTCACCGAGAAATTGGGAGATGCTCTCAAGACAAAATCAGCTGCAAAAGCTCACCGGAAACCTCGACTAAGAATTGGAGAAAGACTCCATTTGCTTGAGCTCATTGTTGGACTCTACCTCTTCTTCTGTGGGTGCTATGATCTTGCTTATGGAAAGAACCGCTTCTTCATCTACCTTTTCCTCCAATCCATTGCATTCTTCATCGCCGGGGTTGGTTATGTTGGCACCTTCGTCCCTAGCTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_tranf_2_3 Glyco_trans_2_3

Protein Analysis

512

Amino Acids

58.71

Weight (kDa)

9.32

Isoelectric Point (pI)

36.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_tranf_2_3 PF13641 76 - 311 1e-17 Glycosyltransferase like family 2
Glycos_transf_2 PF00535 81 - 237 5.3e-12 Glycosyl transferase family 2
Glyco_transf_21 PF13506 164 - 309 8.4e-06 Glycosyl transferase family 21
Glyco_trans_2_3 PF13632 169 - 368 6.6e-21 Glycosyl transferase family group 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1517
AccI GTMKAC 1 cut(s) 1134
AccII CGCG 1 cut(s) 8
AccIII TCCGGA 1 cut(s) 948
AciI CCGC 4 cut(s) 53, 74, 787, 1453
AclWI GGATC 1 cut(s) 329
AcsI RAATTY 1 cut(s) 243
AcuI CTGAAG 1 cut(s) 1125
AfaI GTAC 5 cut(s) 119, 254, 449, 819, 889
AfiI CCNNNNNNNGG 5 cut(s) 766, 955, 1124, 1236, 1243
AgsI TTSAA 7 cut(s) 332, 348, 727, 895, 997, 1112, 1268
AleI CACNNNNGTG 1 cut(s) 794
Alw21I GWGCWC 1 cut(s) 1395
Alw26I GTCTC 2 cut(s) 426, 666
AlwI GGATC 1 cut(s) 329
Ama87I CYCGRG 1 cut(s) 11
Aor13HI TCCGGA 1 cut(s) 948
AoxI GGCC 3 cut(s) 308, 756, 1258
ApaI GGGCCC 1 cut(s) 760
ApeKI GCWGC 4 cut(s) 25, 290, 913, 1334
ApoI RAATTY 1 cut(s) 243
Asp700I GAANNNNTTC 1 cut(s) 1454
AspLEI GCGC 3 cut(s) 10, 290, 558
AspS9I GGNCC 4 cut(s) 756, 757, 784, 932
AsuC2I CCSGG 1 cut(s) 1501
AsuHPI GGTGA 4 cut(s) 880, 1048, 1288, 1337
AvaI CYCGRG 1 cut(s) 11
AvaII GGWCC 2 cut(s) 784, 932
BaeGI GKGCMC 1 cut(s) 760
BanI GGYRCC 1 cut(s) 1517
BanII GRGCYC 4 cut(s) 463, 543, 760, 1395
BarI GAAGNNNNNNTAC 2 cut(s) 470, 502
BbsI GAAGAC 2 cut(s) 166, 1057
Bbv12I GWGCWC 1 cut(s) 1395
BbvI GCAGC 4 cut(s) 37, 277, 925, 1321
BccI CCATC 4 cut(s) 387, 771, 915, 949
BcnI CCSGG 1 cut(s) 1501
BcoDI GTCTC 2 cut(s) 426, 666
BfaI CTAG 4 cut(s) 665, 825, 1179, 1530
BfmI CTRYAG 1 cut(s) 1015
BfoI RGCGCY 1 cut(s) 291
BisI GCNGC 5 cut(s) 26, 53, 291, 914, 1335
BlsI GCNGC 5 cut(s) 27, 54, 292, 915, 1336
BmcAI AGTACT 1 cut(s) 889
Bme1390I CCNGG 1 cut(s) 1501
Bme18I GGWCC 2 cut(s) 784, 932
BmeT110I CYCGRG 1 cut(s) 11
BmgT120I GGNCC 4 cut(s) 756, 757, 784, 932
BmiI GGNNCC 3 cut(s) 758, 1101, 1519
BmrFI CCNGG 1 cut(s) 1501
BmrI ACTGGG 1 cut(s) 879
BmsI GCATC 2 cut(s) 511, 1303
BmuI ACTGGG 1 cut(s) 879
BpiI GAAGAC 2 cut(s) 166, 1057
BplI GAGNNNNNCTC 4 cut(s) 1302, 1334, 1362, 1394
BpmI CTGGAG 2 cut(s) 759, 792
Bpu10I CCTNAGC 1 cut(s) 537
BpuEI CTTGAG 3 cut(s) 848, 1304, 1409
BpuMI CCSGG 1 cut(s) 1501
BsaJI CCNNGG 1 cut(s) 1500
BsaWI WCCGGW 3 cut(s) 948, 1236, 1347
BsaXI ACNNNNNCTCC 2 cut(s) 101, 131
Bsc4I CCNNNNNNNGG 5 cut(s) 766, 955, 1124, 1236, 1243
Bse1I ACTGG 1 cut(s) 885
Bse3DI GCAATG 2 cut(s) 588, 1482
BseAI TCCGGA 1 cut(s) 948
BseDI CCNNGG 1 cut(s) 1500
BseGI GGATG 3 cut(s) 93, 202, 782
BseLI CCNNNNNNNGG 5 cut(s) 766, 955, 1124, 1236, 1243
BseMI GCAATG 2 cut(s) 588, 1482
BseMII CTCAG 2 cut(s) 156, 528
BseNI ACTGG 1 cut(s) 885
BseRI GAGGAG 2 cut(s) 563, 1148
BseSI GKGCMC 1 cut(s) 760
BseXI GCAGC 4 cut(s) 37, 277, 925, 1321
BsgI GTGCAG 2 cut(s) 407, 1217
Bsh1236I CGCG 1 cut(s) 8
BshFI GGCC 3 cut(s) 310, 758, 1260
BshNI GGYRCC 1 cut(s) 1517
BsiHKAI GWGCWC 1 cut(s) 1395
BsiHKCI CYCGRG 1 cut(s) 11
BsiSI CCGG 5 cut(s) 935, 949, 1237, 1348, 1500
BslFI GGGAC 3 cut(s) 973, 1213, 1511
BslI CCNNNNNNNGG 5 cut(s) 766, 955, 1124, 1236, 1243
BsmAI GTCTC 2 cut(s) 426, 666
BsmFI GGGAC 3 cut(s) 973, 1213, 1511
BsmI GAATGC 2 cut(s) 654, 1487
BsnI GGCC 3 cut(s) 310, 758, 1260
BsoBI CYCGRG 1 cut(s) 11
Bsp120I GGGCCC 1 cut(s) 756
Bsp1286I GDGCHC 4 cut(s) 463, 543, 760, 1395
Bsp13I TCCGGA 1 cut(s) 948
Bsp1407I TGTACA 2 cut(s) 117, 252
Bsp143I GATC 5 cut(s) 45, 321, 1011, 1181, 1432
BspACI CCGC 4 cut(s) 53, 74, 787, 1453
BspANI GGCC 3 cut(s) 310, 758, 1260
BspCNI CTCAG 2 cut(s) 157, 529
BspEI TCCGGA 1 cut(s) 948
BspFNI CGCG 1 cut(s) 8
BspLI GGNNCC 3 cut(s) 758, 1101, 1519
BspPI GGATC 1 cut(s) 329
BspT107I GGYRCC 1 cut(s) 1517
BsrDI GCAATG 2 cut(s) 588, 1482
BsrGI TGTACA 2 cut(s) 117, 252
BsrI ACTGG 1 cut(s) 885
BssECI CCNNGG 1 cut(s) 1500
BssMI GATC 5 cut(s) 45, 321, 1011, 1181, 1432
BssNAI GTATAC 1 cut(s) 1135
Bst1107I GTATAC 1 cut(s) 1135
Bst4CI ACNGT 6 cut(s) 498, 682, 796, 905, 1073, 1093
Bst6I CTCTTC 2 cut(s) 950, 1418
BstAUI TGTACA 2 cut(s) 117, 252
BstC8I GCNNGC 3 cut(s) 30, 400, 607
BstDEI CTNAG 5 cut(s) 165, 537, 830, 1242, 1361
BstENI CCTNNNNNAGG 1 cut(s) 764
BstF5I GGATG 3 cut(s) 93, 202, 782
BstFNI CGCG 1 cut(s) 8
BstH2I RGCGCY 1 cut(s) 291
BstHHI GCGC 3 cut(s) 10, 290, 558
BstKTI GATC 5 cut(s) 48, 324, 1014, 1184, 1435
BstMAI GTCTC 2 cut(s) 426, 666
BstMBI GATC 5 cut(s) 45, 321, 1011, 1181, 1432
BstMWI GCNNNNNNNGC 6 cut(s) 22, 58, 307, 710, 821, 1340
BstNSI RCATGY 1 cut(s) 297
BstSCI CCNGG 1 cut(s) 1499
BstSFI CTRYAG 1 cut(s) 1015
BstSLI GKGCMC 1 cut(s) 760
BstUI CGCG 1 cut(s) 8
BstV1I GCAGC 4 cut(s) 37, 277, 925, 1321
BstV2I GAAGAC 2 cut(s) 166, 1057
BstZ17I GTATAC 1 cut(s) 1135
BsuRI GGCC 3 cut(s) 310, 758, 1260
BtgZI GCGATG 1 cut(s) 1480
BtsCI GGATG 3 cut(s) 93, 202, 782
BtsIMutI CAGTG 4 cut(s) 41, 503, 687, 801
Cac8I GCNNGC 3 cut(s) 30, 400, 607
CfoI GCGC 3 cut(s) 10, 290, 558
Cfr13I GGNCC 4 cut(s) 756, 757, 784, 932
CseI GACGC 1 cut(s) 14
Csp6I GTAC 5 cut(s) 118, 253, 448, 818, 888
CviAII CATG 5 cut(s) 121, 294, 306, 710, 1007
CviQI GTAC 5 cut(s) 118, 253, 448, 818, 888
DdeI CTNAG 5 cut(s) 165, 537, 830, 1242, 1361
DpnI GATC 5 cut(s) 47, 323, 1013, 1183, 1434
DpnII GATC 5 cut(s) 45, 321, 1011, 1181, 1432
Eam1104I CTCTTC 2 cut(s) 950, 1418
EarI CTCTTC 2 cut(s) 950, 1418
Ecl136II GAGCTC 1 cut(s) 1393
Eco147I AGGCCT 1 cut(s) 1260
Eco24I GRGCYC 4 cut(s) 463, 543, 760, 1395
Eco47I GGWCC 2 cut(s) 784, 932
Eco53kI GAGCTC 1 cut(s) 1393
Eco57I CTGAAG 1 cut(s) 1125
Eco88I CYCGRG 1 cut(s) 11
EcoICRI GAGCTC 1 cut(s) 1393
EcoNI CCTNNNNNAGG 1 cut(s) 764
EcoO109I RGGNCCY 2 cut(s) 756, 757
EcoT38I GRGCYC 4 cut(s) 463, 543, 760, 1395
FaeI CATG 5 cut(s) 124, 297, 309, 713, 1010
FalI AAGNNNNNCTT 2 cut(s) 1439, 1471
FaqI GGGAC 3 cut(s) 973, 1213, 1511
FatI CATG 5 cut(s) 120, 293, 305, 709, 1006
FblI GTMKAC 1 cut(s) 1134
Fnu4HI GCNGC 5 cut(s) 26, 53, 291, 914, 1335
FokI GGATG 3 cut(s) 100, 209, 789
FriOI GRGCYC 4 cut(s) 463, 543, 760, 1395
Fsp4HI GCNGC 5 cut(s) 26, 53, 291, 914, 1335
FspBI CTAG 4 cut(s) 665, 825, 1179, 1530
GlaI GCGC 3 cut(s) 9, 289, 557
GluI GCNGC 5 cut(s) 26, 53, 291, 914, 1335
GsuI CTGGAG 2 cut(s) 759, 792
HaeII RGCGCY 1 cut(s) 291
HaeIII GGCC 3 cut(s) 310, 758, 1260
HapII CCGG 5 cut(s) 935, 949, 1237, 1348, 1500
HgaI GACGC 1 cut(s) 14
HhaI GCGC 3 cut(s) 10, 290, 558
Hin1II CATG 5 cut(s) 124, 297, 309, 713, 1010
Hin6I GCGC 3 cut(s) 8, 288, 556
HinP1I GCGC 3 cut(s) 8, 288, 556
HinfI GANTC 6 cut(s) 344, 706, 750, 1207, 1377, 1404
HpaII CCGG 5 cut(s) 935, 949, 1237, 1348, 1500
HphI GGTGA 4 cut(s) 880, 1048, 1288, 1337
Hpy166II GTNNAC 2 cut(s) 118, 1135
Hpy188I TCNGA 4 cut(s) 155, 316, 431, 859
Hpy188III TCNNGA 6 cut(s) 338, 665, 949, 1103, 1204, 1321
Hpy8I GTNNAC 2 cut(s) 118, 1135
Hpy99I CGWCG 1 cut(s) 512
HpyAV CCTTC 7 cut(s) 106, 321, 724, 775, 976, 1152, 1531
HpyCH4III ACNGT 6 cut(s) 498, 682, 796, 905, 1073, 1093
HpyCH4IV ACGT 1 cut(s) 507
HpyCH4V TGCA 9 cut(s) 25, 241, 293, 388, 652, 1166, 1234, 1337, 1487
HpyF10VI GCNNNNNNNGC 6 cut(s) 22, 58, 307, 710, 821, 1340
HpyF3I CTNAG 5 cut(s) 165, 537, 830, 1242, 1361
HpySE526I ACGT 1 cut(s) 507
Hsp92II CATG 5 cut(s) 124, 297, 309, 713, 1010
HspAI GCGC 3 cut(s) 8, 288, 556
Kpn2I TCCGGA 1 cut(s) 948
Kzo9I GATC 5 cut(s) 45, 321, 1011, 1181, 1432
LmnI GCTCC 2 cut(s) 205, 685
Lsp1109I GCAGC 4 cut(s) 37, 277, 925, 1321
LweI GCATC 2 cut(s) 511, 1303
MaeI CTAG 4 cut(s) 665, 825, 1179, 1530
MaeII ACGT 1 cut(s) 507
MaeIII GTNAC 3 cut(s) 500, 1054, 1294
MalI GATC 5 cut(s) 47, 323, 1013, 1183, 1434
MboI GATC 5 cut(s) 45, 321, 1011, 1181, 1432
MhlI GDGCHC 4 cut(s) 463, 543, 760, 1395
MluCI AATT 7 cut(s) 126, 189, 214, 243, 878, 1304, 1365
MlyI GAGTC 3 cut(s) 700, 1371, 1398
MmeI TCCRAC 4 cut(s) 155, 556, 1150, 1381
MroI TCCGGA 1 cut(s) 948
MroXI GAANNNNTTC 1 cut(s) 1454
MseI TTAA 3 cut(s) 192, 363, 971
MslI CAYNNNNRTG 1 cut(s) 794
MspA1I CMGCKG 4 cut(s) 28, 55, 737, 1334
MspI CCGG 5 cut(s) 935, 949, 1237, 1348, 1500
MspR9I CCNGG 1 cut(s) 1501
Mva1269I GAATGC 2 cut(s) 654, 1487
MvnI CGCG 1 cut(s) 8
MwoI GCNNNNNNNGC 6 cut(s) 22, 58, 307, 710, 821, 1340
NciI CCSGG 1 cut(s) 1501
NdeII GATC 5 cut(s) 45, 321, 1011, 1181, 1432
NlaIII CATG 5 cut(s) 124, 297, 309, 713, 1010
NlaIV GGNNCC 3 cut(s) 758, 1101, 1519
NmuCI GTSAC 3 cut(s) 500, 1054, 1294
NspI RCATGY 1 cut(s) 297
OliI CACNNNNGTG 1 cut(s) 794
PceI AGGCCT 1 cut(s) 1260
PctI GAATGC 2 cut(s) 654, 1487
PdmI GAANNNNTTC 1 cut(s) 1454
PfeI GAWTC 3 cut(s) 344, 750, 1207
PflFI GACNNNGTC 1 cut(s) 506
PkrI GCNGC 5 cut(s) 27, 54, 292, 915, 1336
PleI GAGTC 3 cut(s) 700, 1371, 1398
PpsI GAGTC 3 cut(s) 700, 1371, 1398
Psp124BI GAGCTC 1 cut(s) 1395
PspN4I GGNNCC 3 cut(s) 758, 1101, 1519
PspOMI GGGCCC 1 cut(s) 756
PspPI GGNCC 4 cut(s) 756, 757, 784, 932
PsrI GAACNNNNNNTAC 2 cut(s) 318, 350
PsyI GACNNNGTC 1 cut(s) 506
PvuII CAGCTG 3 cut(s) 28, 737, 1334
RsaI GTAC 5 cut(s) 119, 254, 449, 819, 889
RsaNI GTAC 5 cut(s) 118, 253, 448, 818, 888
RseI CAYNNNNRTG 1 cut(s) 794
SacI GAGCTC 1 cut(s) 1395
SaqAI TTAA 3 cut(s) 192, 363, 971
SatI GCNGC 5 cut(s) 26, 53, 291, 914, 1335
Sau3AI GATC 5 cut(s) 45, 321, 1011, 1181, 1432
Sau96I GGNCC 4 cut(s) 756, 757, 784, 932
ScaI AGTACT 1 cut(s) 889
SchI GAGTC 3 cut(s) 700, 1371, 1398
ScrFI CCNGG 1 cut(s) 1501
SduI GDGCHC 4 cut(s) 463, 543, 760, 1395
SfaNI GCATC 2 cut(s) 511, 1303
SfcI CTRYAG 1 cut(s) 1015
SinI GGWCC 2 cut(s) 784, 932
SmiMI CAYNNNNRTG 1 cut(s) 794
SmlI CTYRAG 3 cut(s) 863, 1319, 1388
SmoI CTYRAG 3 cut(s) 863, 1319, 1388
Sse9I AATT 7 cut(s) 126, 189, 214, 243, 878, 1304, 1365
SseBI AGGCCT 1 cut(s) 1260
SsiI CCGC 4 cut(s) 53, 74, 787, 1453
SspMI CTAG 4 cut(s) 665, 825, 1179, 1530
SstI GAGCTC 1 cut(s) 1395
StuI AGGCCT 1 cut(s) 1260
StyD4I CCNGG 1 cut(s) 1499
TaaI ACNGT 6 cut(s) 498, 682, 796, 905, 1073, 1093
TaiI ACGT 1 cut(s) 510
TaqI TCGA 1 cut(s) 1357
TasI AATT 7 cut(s) 126, 189, 214, 243, 878, 1304, 1365
TatI WGTACW 3 cut(s) 117, 252, 887
TauI GCSGC 1 cut(s) 55
TfiI GAWTC 3 cut(s) 344, 750, 1207
Tru1I TTAA 3 cut(s) 192, 363, 971
Tru9I TTAA 3 cut(s) 192, 363, 971
TscAI CASTG 4 cut(s) 48, 503, 687, 801
TseFI GTSAC 3 cut(s) 500, 1054, 1294
TseI GCWGC 4 cut(s) 25, 290, 913, 1334
Tsp45I GTSAC 3 cut(s) 500, 1054, 1294
TspDTI ATGAA 8 cut(s) 273, 294, 491, 891, 995, 1299, 1450, 1483
TspRI CASTG 4 cut(s) 48, 503, 687, 801
Tth111I GACNNNGTC 1 cut(s) 506
VpaK11BI GGWCC 2 cut(s) 784, 932
XagI CCTNNNNNAGG 1 cut(s) 764
XapI RAATTY 1 cut(s) 243
XbaI TCTAGA 1 cut(s) 664
XceI RCATGY 1 cut(s) 297
XmiI GTMKAC 1 cut(s) 1134
XmnI GAANNNNTTC 1 cut(s) 1454
XspI CTAG 4 cut(s) 665, 825, 1179, 1530
ZrmI AGTACT 1 cut(s) 889
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.