Rroxscaffold_5G00344170

KH-domain-like of EngA bacterial GTPase enzymes, C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0010592
Physical Location & Seq
Reverse (-)
14128183 .. 14136249
8067 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00344170.1

Sequence Viewer

Length: 798 bp
ATGAGGTATTTGCCTCTTCGACTATCACGGCGTAGCCTAGACATCGGACTACGGGATGCATGTCATGGTTGGGTCCCACCATGGCTTGTAGGTCCCCCAAATAGGGAGTCCATTAGAAATGCTCTTAATTCAACTACAGATCTCAAAACTACTTTAGCAGGTTCAAGCAGCACTCAGGAAATTCCTTTGGGTGAGGATTTGATTGAGTGTGCAAAATGCAAGAAATGTGAAGCAATACTCAAATGTGATTTGACAACAGGGACATGTAGCATGTTACGTCATCACAATTCACGTTCTACTTCCTCTGCTAATAATGTTGTTTCACCACAATCTGCCATACGTTCTACCAAGTTTGAGTATGAGAGATTCTATGAGTTATGGATAGAAGCTATTATCGGACATGATTTACCTTTCAGCTTTGTTGAGTATGAGGATATTAGAGATAGCCTCGTCGTATATGTCGCGGACTTCGAGGCTCGAAAAGGAGCATTTTCTATTCCGAAGCGGTGCGGTTCGGGTTTCGAAGGGCCTTCTCGGGTATCGGGTATTGCGGGGAACAAGGCAATTGTAGTCAATGAACAATGGGTACTAGGGATCGTACGTATGGTAGATCCTTTGGGGGACTTTGAATTTATGGCGGTCGATATTGGTGGTGTTCTTACAATTTCAAATTCACAGGGCCATGTCATGGAAGAAATGGTCATTTCAACGACCATTGGTATGGATGGAATTTTACTTGCCTCCAAAGAGGCAGCTGTTGAAGAGTCATCTGTCATTATTTTTCTTGTTGATGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KH_dom-like MMR_HSR1

Protein Analysis

265

Amino Acids

29.09

Weight (kDa)

5.23

Isoelectric Point (pI)

50.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 149
AccB7I CCANNNNNTGG 1 cut(s) 688
AccII CGCG 1 cut(s) 464
AciI CCGC 5 cut(s) 464, 505, 510, 551, 638
AclWI GGATC 2 cut(s) 602, 605
AcsI RAATTY 4 cut(s) 180, 629, 670, 729
AfaI GTAC 2 cut(s) 588, 600
AfiI CCNNNNNNNGG 3 cut(s) 102, 103, 688
AflIII ACRYGT 1 cut(s) 263
AgsI TTSAA 6 cut(s) 132, 165, 629, 669, 708, 761
AluBI AGCT 3 cut(s) 389, 417, 755
AluI AGCT 3 cut(s) 389, 417, 755
AlwI GGATC 2 cut(s) 602, 605
Ama87I CYCGRG 1 cut(s) 534
AoxI GGCC 2 cut(s) 527, 679
ApeKI GCWGC 2 cut(s) 168, 752
ApoI RAATTY 4 cut(s) 180, 629, 670, 729
ArsI GACNNNNNNTTYG 2 cut(s) 92, 124
AspS9I GGNCC 4 cut(s) 73, 92, 527, 679
AsuHPI GGTGA 2 cut(s) 203, 315
AsuII TTCGAA 1 cut(s) 522
AvaI CYCGRG 1 cut(s) 534
AvaII GGWCC 2 cut(s) 73, 92
BbvI GCAGC 2 cut(s) 180, 764
BccI CCATC 2 cut(s) 719, 785
BceAI ACGGC 1 cut(s) 44
BfaI CTAG 3 cut(s) 38, 590, 796
BfmI CTRYAG 1 cut(s) 135
BfuAI ACCTGC 1 cut(s) 149
BglII AGATCT 1 cut(s) 139
BisI GCNGC 2 cut(s) 169, 753
BlsI GCNGC 2 cut(s) 170, 754
Bme18I GGWCC 2 cut(s) 73, 92
BmeT110I CYCGRG 1 cut(s) 534
BmgT120I GGNCC 4 cut(s) 73, 92, 527, 679
BmiI GGNNCC 3 cut(s) 74, 75, 94
BmsI GCATC 1 cut(s) 46
BplI GAGNNNNNCTC 2 cut(s) 432, 464
Bpu14I TTCGAA 1 cut(s) 522
BsaAI YACGTR 1 cut(s) 602
BsaJI CCNNGG 1 cut(s) 80
Bsc4I CCNNNNNNNGG 3 cut(s) 102, 103, 688
BseDI CCNNGG 1 cut(s) 80
BseGI GGATG 2 cut(s) 61, 730
BseLI CCNNNNNNNGG 3 cut(s) 102, 103, 688
BseMII CTCAG 1 cut(s) 188
BseXI GCAGC 2 cut(s) 180, 764
Bsh1236I CGCG 1 cut(s) 464
Bsh1285I CGRYCG 1 cut(s) 642
BshFI GGCC 2 cut(s) 529, 681
BsiEI CGRYCG 1 cut(s) 642
BsiHKCI CYCGRG 1 cut(s) 534
BsiWI CGTACG 1 cut(s) 598
BslFI GGGAC 4 cut(s) 59, 78, 274, 635
BslI CCNNNNNNNGG 3 cut(s) 102, 103, 688
BsmFI GGGAC 4 cut(s) 59, 78, 274, 635
BsnI GGCC 2 cut(s) 529, 681
BsoBI CYCGRG 1 cut(s) 534
Bsp119I TTCGAA 1 cut(s) 522
Bsp143I GATC 3 cut(s) 139, 594, 610
Bsp19I CCATGG 1 cut(s) 80
BspACI CCGC 5 cut(s) 464, 505, 510, 551, 638
BspANI GGCC 2 cut(s) 529, 681
BspCNI CTCAG 1 cut(s) 187
BspFNI CGCG 1 cut(s) 464
BspLI GGNNCC 3 cut(s) 74, 75, 94
BspMI ACCTGC 1 cut(s) 149
BspPI GGATC 2 cut(s) 602, 605
BspT104I TTCGAA 1 cut(s) 522
BssECI CCNNGG 1 cut(s) 80
BssMI GATC 3 cut(s) 139, 594, 610
BssT1I CCWWGG 1 cut(s) 80
Bst6I CTCTTC 2 cut(s) 21, 756
BstBAI YACGTR 1 cut(s) 602
BstBI TTCGAA 1 cut(s) 522
BstDEI CTNAG 1 cut(s) 174
BstDSI CCRYGG 1 cut(s) 80
BstF5I GGATG 2 cut(s) 61, 730
BstFNI CGCG 1 cut(s) 464
BstKTI GATC 3 cut(s) 142, 597, 613
BstMBI GATC 3 cut(s) 139, 594, 610
BstMCI CGRYCG 1 cut(s) 642
BstNSI RCATGY 3 cut(s) 63, 267, 274
BstSFI CTRYAG 1 cut(s) 135
BstSNI TACGTA 1 cut(s) 602
BstUI CGCG 1 cut(s) 464
BstV1I GCAGC 2 cut(s) 180, 764
BstX2I RGATCY 2 cut(s) 139, 610
BstXI CCANNNNNNTGG 1 cut(s) 721
BstYI RGATCY 2 cut(s) 139, 610
BsuRI GGCC 2 cut(s) 529, 681
BtgI CCRYGG 1 cut(s) 80
BtsCI GGATG 2 cut(s) 61, 730
BveI ACCTGC 1 cut(s) 149
Cfr13I GGNCC 4 cut(s) 73, 92, 527, 679
Csp6I GTAC 2 cut(s) 587, 599
CviAII CATG 8 cut(s) 60, 65, 81, 264, 271, 401, 683, 688
CviQI GTAC 2 cut(s) 587, 599
DdeI CTNAG 1 cut(s) 174
DpnI GATC 3 cut(s) 141, 596, 612
DpnII GATC 3 cut(s) 139, 594, 610
Eam1104I CTCTTC 2 cut(s) 21, 756
EarI CTCTTC 2 cut(s) 21, 756
Eco105I TACGTA 1 cut(s) 602
Eco130I CCWWGG 1 cut(s) 80
Eco47I GGWCC 2 cut(s) 73, 92
Eco88I CYCGRG 1 cut(s) 534
EcoO109I RGGNCCY 3 cut(s) 73, 92, 527
EcoT14I CCWWGG 1 cut(s) 80
EcoT22I ATGCAT 1 cut(s) 61
ErhI CCWWGG 1 cut(s) 80
FaeI CATG 8 cut(s) 63, 68, 84, 267, 274, 404, 686, 691
FaqI GGGAC 4 cut(s) 59, 78, 274, 635
FatI CATG 8 cut(s) 59, 64, 80, 263, 270, 400, 682, 687
FauI CCCGC 1 cut(s) 544
Fnu4HI GCNGC 2 cut(s) 169, 753
FokI GGATG 2 cut(s) 68, 737
Fsp4HI GCNGC 2 cut(s) 169, 753
FspBI CTAG 3 cut(s) 38, 590, 796
GluI GCNGC 2 cut(s) 169, 753
HaeIII GGCC 2 cut(s) 529, 681
Hin1II CATG 8 cut(s) 63, 68, 84, 267, 274, 404, 686, 691
HinfI GANTC 3 cut(s) 107, 366, 764
HphI GGTGA 2 cut(s) 203, 315
Hpy188I TCNGA 3 cut(s) 47, 398, 501
Hpy188III TCNNGA 1 cut(s) 176
Hpy99I CGWCG 1 cut(s) 455
HpyAV CCTTC 2 cut(s) 518, 540
HpyCH4IV ACGT 4 cut(s) 277, 292, 340, 601
HpyCH4V TGCA 3 cut(s) 59, 212, 219
HpyF3I CTNAG 1 cut(s) 174
HpySE526I ACGT 4 cut(s) 277, 292, 340, 601
Hsp92II CATG 8 cut(s) 63, 68, 84, 267, 274, 404, 686, 691
KflI GGGWCCC 1 cut(s) 73
Kzo9I GATC 3 cut(s) 139, 594, 610
LmnI GCTCC 1 cut(s) 485
LpnPI CCDG 4 cut(s) 144, 161, 243, 662
Lsp1109I GCAGC 2 cut(s) 180, 764
LweI GCATC 1 cut(s) 46
MaeI CTAG 3 cut(s) 38, 590, 796
MaeII ACGT 4 cut(s) 277, 292, 340, 601
MaeIII GTNAC 1 cut(s) 273
MalI GATC 3 cut(s) 141, 596, 612
MboI GATC 3 cut(s) 139, 594, 610
MboII GAAGA 3 cut(s) 8, 704, 773
MfeI CAATTG 1 cut(s) 564
MflI RGATCY 2 cut(s) 139, 610
MluCI AATT 8 cut(s) 127, 180, 286, 564, 629, 663, 670, 729
MlyI GAGTC 2 cut(s) 116, 773
MnlI CCTC 8 cut(s) 24, 187, 313, 424, 458, 466, 742, 751
Mph1103I ATGCAT 1 cut(s) 61
MseI TTAA 1 cut(s) 126
MslI CAYNNNNRTG 1 cut(s) 719
MspA1I CMGCKG 1 cut(s) 755
MunI CAATTG 1 cut(s) 564
MvnI CGCG 1 cut(s) 464
NcoI CCATGG 1 cut(s) 80
NdeII GATC 3 cut(s) 139, 594, 610
NlaIII CATG 8 cut(s) 63, 68, 84, 267, 274, 404, 686, 691
NlaIV GGNNCC 3 cut(s) 74, 75, 94
NsiI ATGCAT 1 cut(s) 61
NspI RCATGY 3 cut(s) 63, 267, 274
NspV TTCGAA 1 cut(s) 522
PciI ACATGT 1 cut(s) 263
PcsI WCGNNNNNNNCGW 1 cut(s) 468
PfeI GAWTC 1 cut(s) 366
Pfl23II CGTACG 1 cut(s) 598
PflMI CCANNNNNTGG 1 cut(s) 688
PkrI GCNGC 2 cut(s) 170, 754
PleI GAGTC 2 cut(s) 115, 772
PpsI GAGTC 2 cut(s) 115, 772
Ppu21I YACGTR 1 cut(s) 602
PpuMI RGGWCCY 2 cut(s) 73, 92
PscI ACATGT 1 cut(s) 263
Psp5II RGGWCCY 2 cut(s) 73, 92
PspLI CGTACG 1 cut(s) 598
PspN4I GGNNCC 3 cut(s) 74, 75, 94
PspPI GGNCC 4 cut(s) 73, 92, 527, 679
PspPPI RGGWCCY 2 cut(s) 73, 92
PsrI GAACNNNNNNTAC 2 cut(s) 570, 602
PsuI RGATCY 2 cut(s) 139, 610
PvuII CAGCTG 1 cut(s) 755
RsaI GTAC 2 cut(s) 588, 600
RsaNI GTAC 2 cut(s) 587, 599
RseI CAYNNNNRTG 1 cut(s) 719
SaqAI TTAA 1 cut(s) 126
SatI GCNGC 2 cut(s) 169, 753
Sau3AI GATC 3 cut(s) 139, 594, 610
Sau96I GGNCC 4 cut(s) 73, 92, 527, 679
SchI GAGTC 2 cut(s) 116, 773
SfaNI GCATC 1 cut(s) 46
SfcI CTRYAG 1 cut(s) 135
SfuI TTCGAA 1 cut(s) 522
SinI GGWCC 2 cut(s) 73, 92
SmiMI CAYNNNNRTG 1 cut(s) 719
SnaBI TACGTA 1 cut(s) 602
Sse9I AATT 8 cut(s) 127, 180, 286, 564, 629, 663, 670, 729
SsiI CCGC 5 cut(s) 464, 505, 510, 551, 638
SspMI CTAG 3 cut(s) 38, 590, 796
StyI CCWWGG 1 cut(s) 80
TaiI ACGT 4 cut(s) 280, 295, 343, 604
TaqI TCGA 5 cut(s) 19, 471, 478, 522, 642
TasI AATT 8 cut(s) 127, 180, 286, 564, 629, 663, 670, 729
TfiI GAWTC 1 cut(s) 366
Tru1I TTAA 1 cut(s) 126
Tru9I TTAA 1 cut(s) 126
TseI GCWGC 2 cut(s) 168, 752
TspDTI ATGAA 1 cut(s) 591
Van91I CCANNNNNTGG 1 cut(s) 688
VpaK11BI GGWCC 2 cut(s) 73, 92
XapI RAATTY 4 cut(s) 180, 629, 670, 729
XceI RCATGY 3 cut(s) 63, 267, 274
XspI CTAG 3 cut(s) 38, 590, 796
Zsp2I ATGCAT 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.