Rorug05G0321700

KH-domain-like of EngA bacterial GTPase enzymes, C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0010592
Physical Location & Seq
Reverse (-)
38354347 .. 38357018
2672 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0321700.1

Sequence Viewer

Length: 612 bp
ATGGGTTTTGGATTCATTGAAGTATCGAGCTCGTGGTCAACTGGTCTTGCTCTTGGAGTCTTGGTTCGATTCAGAAATCTTCAAGCTCAGGATCCAAAGGAGGAGGCTAAAGCAGAGACAAGGGATTGGATAAACACTGTGGATGATTCTGCTGCACTTAGGAGTGCTGCTTCTACCTGGCACGCTGAACTGGCACCGCTCCTGAAGCCCTTGCTTGTAGTCAATATAGGAGGCCCTTCTGAATGCAGTAGTAGTAATGGCAGATTGGGGACCGGTGGTGATAGCGGTGGTGTTGTTTGTGCTGTTAAGTCCAGGGCTACTATTTCAGCTGCTGGGGAAGAGCAGAGTGGTGGAGTTTGGCAACATGCAAACCAGCGGACTATAGTTGTAAAATACATCTCCCTTGATGAAGCTGCAAGGTTATTACTTGGGGATGCACACAATGCATCCGTAATAAGAACTAAAGTAAGGCGGATTTATGATATTGCAAATGTTTCGTCCTCCATGAATCTTATTGAAAAGACCCATGCAGCAGATACAAGGAAGCCTGCATATAAATGGTCTTTCAACCTGTCTTTTGGTGAGGAATGTTACAAGCAAGGAGGATGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KH_dom-like MMR_HSR1

Protein Analysis

203

Amino Acids

21.77

Weight (kDa)

7.69

Isoelectric Point (pI)

48.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_E2F_TDP PF02319 132 - 187 5.6e-10 E2F/DP family winged-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 193
AccBSI CCGCTC 1 cut(s) 199
AciI CCGC 4 cut(s) 197, 285, 376, 472
AclWI GGATC 2 cut(s) 86, 99
AcuI CTGAAG 1 cut(s) 224
AgeI ACCGGT 1 cut(s) 272
AgsI TTSAA 4 cut(s) 20, 83, 518, 568
AjnI CCWGG 2 cut(s) 176, 311
AloI GAACNNNNNNTCC 2 cut(s) 48, 80
AluBI AGCT 4 cut(s) 30, 86, 329, 413
AluI AGCT 4 cut(s) 30, 86, 329, 413
Alw21I GWGCWC 1 cut(s) 32
Alw26I GTCTC 1 cut(s) 110
AlwI GGATC 2 cut(s) 86, 99
AlwNI CAGNNNCTG 1 cut(s) 332
AoxI GGCC 1 cut(s) 232
ApeKI GCWGC 5 cut(s) 152, 167, 329, 413, 530
ArsI GACNNNNNNTTYG 2 cut(s) 482, 514
AsiGI ACCGGT 1 cut(s) 272
AspS9I GGNCC 2 cut(s) 233, 270
AsuHPI GGTGA 2 cut(s) 290, 593
AvaII GGWCC 1 cut(s) 270
BamHI GGATCC 1 cut(s) 91
BanI GGYRCC 1 cut(s) 193
BanII GRGCYC 1 cut(s) 32
BauI CACGAG 1 cut(s) 31
Bbv12I GWGCWC 1 cut(s) 32
BbvI GCAGC 5 cut(s) 139, 154, 316, 400, 542
BcgI CGANNNNNNTGC 2 cut(s) 477, 511
BciT130I CCWGG 2 cut(s) 178, 313
BcoDI GTCTC 1 cut(s) 110
BfmI CTRYAG 1 cut(s) 381
BisI GCNGC 5 cut(s) 153, 168, 330, 414, 531
BlsI GCNGC 5 cut(s) 154, 169, 331, 415, 532
Bme1390I CCNGG 2 cut(s) 178, 313
Bme18I GGWCC 1 cut(s) 270
BmgT120I GGNCC 2 cut(s) 233, 270
BmiI GGNNCC 3 cut(s) 93, 195, 271
BmrFI CCNGG 2 cut(s) 178, 313
BmsI GCATC 2 cut(s) 424, 455
Bpu10I CCTNAGC 1 cut(s) 87
BsaJI CCNNGG 1 cut(s) 312
BsaWI WCCGGW 1 cut(s) 272
BsaXI ACNNNNNCTCC 2 cut(s) 48, 78
Bse118I RCCGGY 1 cut(s) 272
Bse1I ACTGG 2 cut(s) 46, 195
BseBI CCWGG 2 cut(s) 178, 313
BseDI CCNNGG 1 cut(s) 312
BseGI GGATG 4 cut(s) 148, 439, 446, 611
BseMII CTCAG 1 cut(s) 101
BseNI ACTGG 2 cut(s) 46, 195
BseRI GAGGAG 1 cut(s) 116
BseXI GCAGC 5 cut(s) 139, 154, 316, 400, 542
BseYI CCCAGC 1 cut(s) 332
BsgI GTGCAG 1 cut(s) 138
BshFI GGCC 1 cut(s) 234
BshNI GGYRCC 1 cut(s) 193
BshTI ACCGGT 1 cut(s) 272
BsiHKAI GWGCWC 1 cut(s) 32
BsiSI CCGG 1 cut(s) 273
BslFI GGGAC 1 cut(s) 283
BsmAI GTCTC 1 cut(s) 110
BsmFI GGGAC 1 cut(s) 283
BsmI GAATGC 1 cut(s) 248
BsnI GGCC 1 cut(s) 234
Bsp1286I GDGCHC 1 cut(s) 32
Bsp143I GATC 1 cut(s) 91
BspACI CCGC 4 cut(s) 197, 285, 376, 472
BspANI GGCC 1 cut(s) 234
BspCNI CTCAG 1 cut(s) 100
BspLI GGNNCC 3 cut(s) 93, 195, 271
BspPI GGATC 2 cut(s) 86, 99
BspQI GCTCTTC 1 cut(s) 333
BspT107I GGYRCC 1 cut(s) 193
BsrBI CCGCTC 1 cut(s) 199
BsrFI RCCGGY 1 cut(s) 272
BsrI ACTGG 2 cut(s) 46, 195
BssAI RCCGGY 1 cut(s) 272
BssECI CCNNGG 1 cut(s) 312
BssMI GATC 1 cut(s) 91
BssSI CACGAG 1 cut(s) 31
Bst2BI CACGAG 1 cut(s) 31
Bst2UI CCWGG 2 cut(s) 178, 313
Bst4CI ACNGT 1 cut(s) 139
Bst6I CTCTTC 1 cut(s) 333
BstAPI GCANNNNNTGC 1 cut(s) 443
BstC8I GCNNGC 2 cut(s) 183, 549
BstDEI CTNAG 2 cut(s) 87, 158
BstF5I GGATG 4 cut(s) 148, 439, 446, 611
BstKTI GATC 1 cut(s) 94
BstMAI GTCTC 1 cut(s) 110
BstMBI GATC 1 cut(s) 91
BstMWI GCNNNNNNNGC 3 cut(s) 191, 205, 443
BstNI CCWGG 2 cut(s) 178, 313
BstNSI RCATGY 1 cut(s) 368
BstSCI CCNGG 2 cut(s) 176, 311
BstSFI CTRYAG 1 cut(s) 381
BstV1I GCAGC 5 cut(s) 139, 154, 316, 400, 542
BstX2I RGATCY 1 cut(s) 91
BstYI RGATCY 1 cut(s) 91
BsuRI GGCC 1 cut(s) 234
BtsCI GGATG 4 cut(s) 148, 439, 446, 611
BtsIMutI CAGTG 1 cut(s) 135
Cac8I GCNNGC 2 cut(s) 183, 549
CaiI CAGNNNCTG 1 cut(s) 332
Cfr10I RCCGGY 1 cut(s) 272
Cfr13I GGNCC 2 cut(s) 233, 270
CspAI ACCGGT 1 cut(s) 272
CviAII CATG 3 cut(s) 365, 505, 527
CviJI RGCY 9 cut(s) 30, 86, 107, 208, 234, 317, 329, 413, 547
CviKI_1 RGCY 9 cut(s) 30, 86, 107, 208, 234, 317, 329, 413, 547
DdeI CTNAG 2 cut(s) 87, 158
DpnI GATC 1 cut(s) 93
DpnII GATC 1 cut(s) 91
Eam1104I CTCTTC 1 cut(s) 333
EarI CTCTTC 1 cut(s) 333
EciI GGCGGA 1 cut(s) 487
Ecl136II GAGCTC 1 cut(s) 30
Eco24I GRGCYC 1 cut(s) 32
Eco47I GGWCC 1 cut(s) 270
Eco53kI GAGCTC 1 cut(s) 30
Eco57I CTGAAG 1 cut(s) 224
EcoICRI GAGCTC 1 cut(s) 30
EcoO109I RGGNCCY 1 cut(s) 233
EcoRII CCWGG 2 cut(s) 176, 311
EcoT22I ATGCAT 1 cut(s) 448
EcoT38I GRGCYC 1 cut(s) 32
FaeI CATG 3 cut(s) 368, 508, 530
FaiI YATR 8 cut(s) 227, 366, 383, 480, 506, 528, 553, 555
FaqI GGGAC 1 cut(s) 283
FatI CATG 3 cut(s) 364, 504, 526
Fnu4HI GCNGC 5 cut(s) 153, 168, 330, 414, 531
FokI GGATG 3 cut(s) 155, 433, 446
FriOI GRGCYC 1 cut(s) 32
Fsp4HI GCNGC 5 cut(s) 153, 168, 330, 414, 531
GluI GCNGC 5 cut(s) 153, 168, 330, 414, 531
GsaI CCCAGC 1 cut(s) 336
HaeIII GGCC 1 cut(s) 234
HapII CCGG 1 cut(s) 273
Hin1II CATG 3 cut(s) 368, 508, 530
HincII GTYRAC 1 cut(s) 39
HindII GTYRAC 1 cut(s) 39
HinfI GANTC 5 cut(s) 12, 57, 69, 146, 508
HpaII CCGG 1 cut(s) 273
HphI GGTGA 2 cut(s) 290, 593
Hpy166II GTNNAC 1 cut(s) 39
Hpy188I TCNGA 2 cut(s) 74, 241
Hpy188III TCNNGA 2 cut(s) 89, 202
Hpy8I GTNNAC 1 cut(s) 39
HpyAV CCTTC 1 cut(s) 246
HpyCH4III ACNGT 1 cut(s) 139
HpyCH4V TGCA 9 cut(s) 155, 246, 368, 416, 437, 446, 488, 530, 551
HpyF10VI GCNNNNNNNGC 3 cut(s) 191, 205, 443
HpyF3I CTNAG 2 cut(s) 87, 158
Hsp92II CATG 3 cut(s) 368, 508, 530
Kzo9I GATC 1 cut(s) 91
LguI GCTCTTC 1 cut(s) 333
LmnI GCTCC 1 cut(s) 204
Lsp1109I GCAGC 5 cut(s) 139, 154, 316, 400, 542
LweI GCATC 2 cut(s) 424, 455
MaeIII GTNAC 1 cut(s) 590
MalI GATC 1 cut(s) 93
MbiI CCGCTC 1 cut(s) 199
MboI GATC 1 cut(s) 91
MboII GAAGA 2 cut(s) 71, 350
MflI RGATCY 1 cut(s) 91
MhlI GDGCHC 1 cut(s) 32
MlyI GAGTC 1 cut(s) 66
MnlI CCTC 6 cut(s) 94, 97, 224, 511, 577, 596
Mph1103I ATGCAT 1 cut(s) 448
MseI TTAA 1 cut(s) 306
MslI CAYNNNNRTG 1 cut(s) 556
MspA1I CMGCKG 2 cut(s) 329, 376
MspI CCGG 1 cut(s) 273
MspR9I CCNGG 2 cut(s) 178, 313
Mva1269I GAATGC 1 cut(s) 248
MvaI CCWGG 2 cut(s) 178, 313
MwoI GCNNNNNNNGC 3 cut(s) 191, 205, 443
NdeII GATC 1 cut(s) 91
NlaIII CATG 3 cut(s) 368, 508, 530
NlaIV GGNNCC 3 cut(s) 93, 195, 271
NsiI ATGCAT 1 cut(s) 448
NspI RCATGY 1 cut(s) 368
PciSI GCTCTTC 1 cut(s) 333
PctI GAATGC 1 cut(s) 248
PfeI GAWTC 4 cut(s) 12, 69, 146, 508
PinAI ACCGGT 1 cut(s) 272
PkrI GCNGC 5 cut(s) 154, 169, 331, 415, 532
PleI GAGTC 1 cut(s) 65
PpsI GAGTC 1 cut(s) 65
Psp124BI GAGCTC 1 cut(s) 32
Psp6I CCWGG 2 cut(s) 176, 311
PspFI CCCAGC 1 cut(s) 332
PspGI CCWGG 2 cut(s) 176, 311
PspN4I GGNNCC 3 cut(s) 93, 195, 271
PspPI GGNCC 2 cut(s) 233, 270
PstNI CAGNNNCTG 1 cut(s) 332
PsuI RGATCY 1 cut(s) 91
PvuII CAGCTG 1 cut(s) 329
RseI CAYNNNNRTG 1 cut(s) 556
SacI GAGCTC 1 cut(s) 32
SapI GCTCTTC 1 cut(s) 333
SaqAI TTAA 1 cut(s) 306
SatI GCNGC 5 cut(s) 153, 168, 330, 414, 531
Sau3AI GATC 1 cut(s) 91
Sau96I GGNCC 2 cut(s) 233, 270
SchI GAGTC 1 cut(s) 66
ScrFI CCNGG 2 cut(s) 178, 313
SduI GDGCHC 1 cut(s) 32
SetI ASST 7 cut(s) 32, 88, 179, 331, 415, 422, 573
SfaNI GCATC 2 cut(s) 424, 455
SfcI CTRYAG 1 cut(s) 381
SinI GGWCC 1 cut(s) 270
SmiMI CAYNNNNRTG 1 cut(s) 556
SsiI CCGC 4 cut(s) 197, 285, 376, 472
SstI GAGCTC 1 cut(s) 32
StyD4I CCNGG 2 cut(s) 176, 311
TaaI ACNGT 1 cut(s) 139
TaqI TCGA 2 cut(s) 26, 67
TfiI GAWTC 4 cut(s) 12, 69, 146, 508
Tru1I TTAA 1 cut(s) 306
Tru9I TTAA 1 cut(s) 306
TscAI CASTG 1 cut(s) 142
TseI GCWGC 5 cut(s) 152, 167, 329, 413, 530
TspDTI ATGAA 3 cut(s) 4, 423, 521
TspGWI ACGGA 1 cut(s) 439
TspRI CASTG 1 cut(s) 142
VpaK11BI GGWCC 1 cut(s) 270
XceI RCATGY 1 cut(s) 368
Zsp2I ATGCAT 1 cut(s) 448
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.