Rroxscaffold_7G00200370

KH-domain-like of EngA bacterial GTPase enzymes, C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0010592
Physical Location & Seq
Forward (+)
47724780 .. 47742382
17603 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00200370.1

Sequence Viewer

Length: 591 bp
ATGATGGAAAATGTCACATTAGAGAGTGAAATGATAAAAATGTCACCTAGTTTCCCACTTGATAAAAAGGTCCATAATGAATTTGTCCCCAACTTCTCTATCTCCGATGTCGTCCAAAACCTACGCCTCGATGTCCTCCAAGTCTCCTTCCAAATCGAACAGTCGCCGATCACCGCCGCTCTCTCCAAGTTCTTCTTCTACGTCCTCTCTCATAGCCTCGTTGTCGATGTCGCGGACTTCGAGGCTCCGAAACTGCGCGGTTTCGATTCGGAAGCGATCTTGTTTGGGTTTCGAAGTGGGTCCCGCAGGGCCACTTCGGATTCCGGGGTGGAATCCGGGGCTGGTCTTGTCATTCTTACAATTTCAAATTCACGGGGCCATGTCATGGAAGAAATGGTCATTTCAACGACCATTGGTATGGATGGAATTTCACTTGCCTCCAAAGAGGCAGTTGTTGAAGAGTCATCTGTCATTATTTTTCTTGTTGATGGCCAGTGGTCGAATCAAGCCGGTGAAGGAAAGCCCCGAAACTTCAAGAACCCTAGAAATGAAAAATTGTCAATTCGACCTCTACAATGCAAATTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KH_dom-like MMR_HSR1

Protein Analysis

196

Amino Acids

21.66

Weight (kDa)

5.18

Isoelectric Point (pI)

46.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 385
AccBSI CCGCTC 1 cut(s) 179
AccII CGCG 2 cut(s) 233, 258
AciI CCGC 5 cut(s) 174, 177, 233, 258, 304
AcoI YGGCCR 1 cut(s) 490
AcsI RAATTY 3 cut(s) 80, 367, 426
AfiI CCNNNNNNNGG 1 cut(s) 385
AgsI TTSAA 4 cut(s) 366, 405, 458, 535
AjuI GAANNNNNNNTTGG 2 cut(s) 179, 211
Alw26I GTCTC 1 cut(s) 148
AoxI GGCC 3 cut(s) 309, 376, 490
ApoI RAATTY 3 cut(s) 80, 367, 426
AspLEI GCGC 1 cut(s) 258
AspS9I GGNCC 4 cut(s) 70, 300, 309, 376
AsuC2I CCSGG 2 cut(s) 325, 337
AsuHPI GGTGA 3 cut(s) 36, 163, 524
AsuII TTCGAA 1 cut(s) 292
AvaII GGWCC 2 cut(s) 70, 300
BalI TGGCCA 1 cut(s) 492
BccI CCATC 2 cut(s) 416, 482
BcnI CCSGG 2 cut(s) 325, 337
BcoDI GTCTC 1 cut(s) 148
BfaI CTAG 2 cut(s) 48, 543
BisI GCNGC 1 cut(s) 177
BlsI GCNGC 1 cut(s) 178
Bme1390I CCNGG 2 cut(s) 325, 337
Bme18I GGWCC 2 cut(s) 70, 300
BmgT120I GGNCC 4 cut(s) 70, 300, 309, 376
BmiI GGNNCC 4 cut(s) 246, 301, 302, 377
BmrFI CCNGG 2 cut(s) 325, 337
Bpu14I TTCGAA 1 cut(s) 292
BpuMI CCSGG 2 cut(s) 325, 337
BsaJI CCNNGG 2 cut(s) 324, 336
Bsc4I CCNNNNNNNGG 1 cut(s) 385
Bse118I RCCGGY 1 cut(s) 509
Bse1I ACTGG 1 cut(s) 493
BseDI CCNNGG 2 cut(s) 324, 336
BseGI GGATG 1 cut(s) 427
BseLI CCNNNNNNNGG 1 cut(s) 385
BseNI ACTGG 1 cut(s) 493
Bsh1236I CGCG 2 cut(s) 233, 258
BshFI GGCC 3 cut(s) 311, 378, 492
BsiSI CCGG 3 cut(s) 324, 336, 510
BslFI GGGAC 2 cut(s) 71, 286
BslI CCNNNNNNNGG 1 cut(s) 385
BsmAI GTCTC 1 cut(s) 148
BsmFI GGGAC 2 cut(s) 71, 286
BsnI GGCC 3 cut(s) 311, 378, 492
Bsp119I TTCGAA 1 cut(s) 292
Bsp143I GATC 2 cut(s) 168, 276
BspACI CCGC 5 cut(s) 174, 177, 233, 258, 304
BspANI GGCC 3 cut(s) 311, 378, 492
BspFNI CGCG 2 cut(s) 233, 258
BspLI GGNNCC 4 cut(s) 246, 301, 302, 377
BspT104I TTCGAA 1 cut(s) 292
BsrBI CCGCTC 1 cut(s) 179
BsrFI RCCGGY 1 cut(s) 509
BsrI ACTGG 1 cut(s) 493
BssAI RCCGGY 1 cut(s) 509
BssECI CCNNGG 2 cut(s) 324, 336
BssMI GATC 2 cut(s) 168, 276
Bst4CI ACNGT 1 cut(s) 162
Bst6I CTCTTC 1 cut(s) 453
BstBI TTCGAA 1 cut(s) 292
BstF5I GGATG 1 cut(s) 427
BstFNI CGCG 2 cut(s) 233, 258
BstHHI GCGC 1 cut(s) 258
BstKTI GATC 2 cut(s) 171, 279
BstMAI GTCTC 1 cut(s) 148
BstMBI GATC 2 cut(s) 168, 276
BstSCI CCNGG 2 cut(s) 323, 335
BstUI CGCG 2 cut(s) 233, 258
BstXI CCANNNNNNTGG 1 cut(s) 418
BsuRI GGCC 3 cut(s) 311, 378, 492
BtsCI GGATG 1 cut(s) 427
BtsIMutI CAGTG 1 cut(s) 500
CfoI GCGC 1 cut(s) 258
Cfr10I RCCGGY 1 cut(s) 509
Cfr13I GGNCC 4 cut(s) 70, 300, 309, 376
CviAII CATG 2 cut(s) 380, 385
CviJI RGCY 8 cut(s) 216, 245, 311, 341, 378, 492, 509, 523
CviKI_1 RGCY 8 cut(s) 216, 245, 311, 341, 378, 492, 509, 523
DpnI GATC 2 cut(s) 170, 278
DpnII GATC 2 cut(s) 168, 276
EaeI YGGCCR 1 cut(s) 490
Eam1104I CTCTTC 1 cut(s) 453
EarI CTCTTC 1 cut(s) 453
Eco47I GGWCC 2 cut(s) 70, 300
EcoO109I RGGNCCY 1 cut(s) 300
FaeI CATG 2 cut(s) 383, 388
FaiI YATR 5 cut(s) 75, 213, 381, 386, 419
FalI AAGNNNNNCTT 2 cut(s) 179, 211
FaqI GGGAC 2 cut(s) 71, 286
FatI CATG 2 cut(s) 379, 384
FauI CCCGC 1 cut(s) 311
Fnu4HI GCNGC 1 cut(s) 177
FokI GGATG 1 cut(s) 434
Fsp4HI GCNGC 1 cut(s) 177
FspBI CTAG 2 cut(s) 48, 543
GlaI GCGC 1 cut(s) 257
GluI GCNGC 1 cut(s) 177
HaeIII GGCC 3 cut(s) 311, 378, 492
HapII CCGG 3 cut(s) 324, 336, 510
HhaI GCGC 1 cut(s) 258
Hin1II CATG 2 cut(s) 383, 388
Hin6I GCGC 1 cut(s) 256
HinP1I GCGC 1 cut(s) 256
HinfI GANTC 5 cut(s) 266, 320, 332, 461, 502
HpaII CCGG 3 cut(s) 324, 336, 510
HphI GGTGA 3 cut(s) 36, 163, 524
Hpy188I TCNGA 4 cut(s) 106, 249, 271, 319
Hpy188III TCNNGA 1 cut(s) 535
HpyAV CCTTC 2 cut(s) 157, 509
HpyCH4III ACNGT 1 cut(s) 162
HpyCH4IV ACGT 1 cut(s) 201
HpyCH4V TGCA 1 cut(s) 579
HpySE526I ACGT 1 cut(s) 201
Hsp92II CATG 2 cut(s) 383, 388
HspAI GCGC 1 cut(s) 256
KflI GGGWCCC 1 cut(s) 300
Kzo9I GATC 2 cut(s) 168, 276
LmnI GCTCC 1 cut(s) 250
LpnPI CCDG 6 cut(s) 292, 327, 337, 349, 506, 523
MaeI CTAG 2 cut(s) 48, 543
MaeII ACGT 1 cut(s) 201
MaeIII GTNAC 2 cut(s) 13, 42
MalI GATC 2 cut(s) 170, 278
MbiI CCGCTC 1 cut(s) 179
MboI GATC 2 cut(s) 168, 276
MboII GAAGA 4 cut(s) 184, 187, 401, 470
MlsI TGGCCA 1 cut(s) 492
MluCI AATT 7 cut(s) 80, 360, 367, 426, 554, 561, 581
MluNI TGGCCA 1 cut(s) 492
MlyI GAGTC 1 cut(s) 470
MnlI CCTC 8 cut(s) 137, 146, 215, 227, 235, 439, 448, 579
Mox20I TGGCCA 1 cut(s) 492
MscI TGGCCA 1 cut(s) 492
MslI CAYNNNNRTG 1 cut(s) 416
Msp20I TGGCCA 1 cut(s) 492
MspI CCGG 3 cut(s) 324, 336, 510
MspR9I CCNGG 2 cut(s) 325, 337
MvnI CGCG 2 cut(s) 233, 258
NciI CCSGG 2 cut(s) 325, 337
NdeII GATC 2 cut(s) 168, 276
NlaIII CATG 2 cut(s) 383, 388
NlaIV GGNNCC 4 cut(s) 246, 301, 302, 377
NmuCI GTSAC 2 cut(s) 13, 42
NspV TTCGAA 1 cut(s) 292
PcsI WCGNNNNNNNCGW 1 cut(s) 237
PfeI GAWTC 4 cut(s) 266, 320, 332, 502
PflMI CCANNNNNTGG 1 cut(s) 385
PkrI GCNGC 1 cut(s) 178
PleI GAGTC 1 cut(s) 469
PpsI GAGTC 1 cut(s) 469
PpuMI RGGWCCY 1 cut(s) 300
Psp5II RGGWCCY 1 cut(s) 300
PspN4I GGNNCC 4 cut(s) 246, 301, 302, 377
PspPI GGNCC 4 cut(s) 70, 300, 309, 376
PspPPI RGGWCCY 1 cut(s) 300
RseI CAYNNNNRTG 1 cut(s) 416
SatI GCNGC 1 cut(s) 177
Sau3AI GATC 2 cut(s) 168, 276
Sau96I GGNCC 4 cut(s) 70, 300, 309, 376
SchI GAGTC 1 cut(s) 470
ScrFI CCNGG 2 cut(s) 325, 337
SetI ASST 5 cut(s) 49, 72, 123, 204, 571
SfuI TTCGAA 1 cut(s) 292
SinI GGWCC 2 cut(s) 70, 300
SmiMI CAYNNNNRTG 1 cut(s) 416
Sse9I AATT 7 cut(s) 80, 360, 367, 426, 554, 561, 581
SsiI CCGC 5 cut(s) 174, 177, 233, 258, 304
SspMI CTAG 2 cut(s) 48, 543
StyD4I CCNGG 2 cut(s) 323, 335
TaaI ACNGT 1 cut(s) 162
TaiI ACGT 1 cut(s) 204
TaqI TCGA 8 cut(s) 129, 156, 225, 240, 264, 292, 500, 565
TasI AATT 7 cut(s) 80, 360, 367, 426, 554, 561, 581
TauI GCSGC 1 cut(s) 179
TfiI GAWTC 4 cut(s) 266, 320, 332, 502
TscAI CASTG 1 cut(s) 500
TseFI GTSAC 2 cut(s) 13, 42
Tsp45I GTSAC 2 cut(s) 13, 42
TspDTI ATGAA 2 cut(s) 93, 564
TspRI CASTG 1 cut(s) 500
Van91I CCANNNNNTGG 1 cut(s) 385
VpaK11BI GGWCC 2 cut(s) 70, 300
XapI RAATTY 3 cut(s) 80, 367, 426
XspI CTAG 2 cut(s) 48, 543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.