Rroxscaffold_5G00345330

Auxin-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0011507
Physical Location & Seq
Forward (+)
15721175 .. 15721813
639 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00345330.1

Sequence Viewer

Length: 639 bp
ATGATTTCCCCTATCTGCCTCTTCGTGTTTTCTTTCATTCTTTCATGTTCCTCTGCTTCGCATGTCCAAGATTTCTGTGTTGCAGACTACGCAGCTCCCCAAGGCCCCGCAGGCTACTCATGCAAAGACCCTGCAAAGGTTACTGTTGATGATTTCGTCCACTCGGGCCTTGGGATGCCTGCTAACACTTCAAATAGGTACAAGTTTGGATTCACATCTGCATTTGCTTTTAACTTCTCCGGCCTCAATGGCCTCGGCGTTTCCTTGGGCCGGACAGACGTGGAAGTTGGTGGTGTTGTCCCCATCCACTCTCACCCGGGAGCTACCGAACTGGTAGTTATTGAGGAAGGAAGTTCGATAATTGGCGGGTTCATTGCCTCGAACAACAAGGTTTATCAAAAGCCCCTGAACAAGGGTGACACTATGGTTCTTCCTCAAGGCTTGTATCACTTCTTTGTGAATCAGGGTAAAACTCCAGCGGTCATATATGTTGCTTTCAGCAGTGAAAGCCCAACCGTGCAGTTTTTGGACACATCACTGTTTAAAAATGATTTGGCTACTGATATCATAGCAAAAACTACTTTACTTGACGCTGCTCAGATTCAGAAACTCAAAACACTCTTTGGTGGTACTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0048046 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

212

Amino Acids

22.53

Weight (kDa)

5.89

Isoelectric Point (pI)

31.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 56 - 201 7.5e-30 Cupin
Cupin_2 PF07883 92 - 165 1.1e-07 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 108, 366, 479
AfaI GTAC 2 cut(s) 200, 631
AfiI CCNNNNNNNGG 3 cut(s) 136, 270, 412
AgsI TTSAA 1 cut(s) 192
AjiI CACGTC 1 cut(s) 280
AluBI AGCT 2 cut(s) 95, 323
AluI AGCT 2 cut(s) 95, 323
Ama87I CYCGRG 2 cut(s) 163, 316
AoxI GGCC 5 cut(s) 103, 166, 241, 250, 268
ApeKI GCWGC 2 cut(s) 92, 593
AspS9I GGNCC 3 cut(s) 104, 166, 268
AsuC2I CCSGG 2 cut(s) 317, 318
AsuHPI GGTGA 2 cut(s) 305, 428
AvaI CYCGRG 2 cut(s) 163, 316
BbvI GCAGC 2 cut(s) 104, 580
BccI CCATC 1 cut(s) 311
BcnI CCSGG 2 cut(s) 317, 318
BglI GCCNNNNNGGC 2 cut(s) 111, 249
BisI GCNGC 2 cut(s) 93, 594
BlsI GCNGC 2 cut(s) 94, 595
Bme1390I CCNGG 2 cut(s) 317, 318
BmeT110I CYCGRG 2 cut(s) 163, 316
BmgBI CACGTC 1 cut(s) 280
BmgT120I GGNCC 3 cut(s) 104, 166, 268
BmiI GGNNCC 1 cut(s) 106
BmrFI CCNGG 2 cut(s) 317, 318
BmsI GCATC 1 cut(s) 165
BpmI CTGGAG 1 cut(s) 459
BpuEI CTTGAG 1 cut(s) 420
BpuMI CCSGG 2 cut(s) 317, 318
BsaBI GATNNNNATC 1 cut(s) 214
BsaJI CCNNGG 5 cut(s) 100, 169, 253, 264, 316
Bsc4I CCNNNNNNNGG 3 cut(s) 136, 270, 412
Bse1I ACTGG 1 cut(s) 336
Bse3DI GCAATG 1 cut(s) 372
Bse8I GATNNNNATC 1 cut(s) 214
BseDI CCNNGG 5 cut(s) 100, 169, 253, 264, 316
BseGI GGATG 2 cut(s) 180, 303
BseJI GATNNNNATC 1 cut(s) 214
BseLI CCNNNNNNNGG 3 cut(s) 136, 270, 412
BseMI GCAATG 1 cut(s) 372
BseMII CTCAG 1 cut(s) 611
BseNI ACTGG 1 cut(s) 336
BseXI GCAGC 2 cut(s) 104, 580
BsgI GTGCAG 1 cut(s) 539
BshFI GGCC 5 cut(s) 105, 168, 243, 252, 270
BsiHKCI CYCGRG 2 cut(s) 163, 316
BsiSI CCGG 3 cut(s) 240, 271, 317
BslFI GGGAC 1 cut(s) 284
BslI CCNNNNNNNGG 3 cut(s) 136, 270, 412
BsmFI GGGAC 1 cut(s) 284
BsnI GGCC 5 cut(s) 105, 168, 243, 252, 270
BsoBI CYCGRG 2 cut(s) 163, 316
BspACI CCGC 3 cut(s) 108, 366, 479
BspANI GGCC 5 cut(s) 105, 168, 243, 252, 270
BspCNI CTCAG 1 cut(s) 610
BspLI GGNNCC 1 cut(s) 106
BsrDI GCAATG 1 cut(s) 372
BsrI ACTGG 1 cut(s) 336
BssECI CCNNGG 5 cut(s) 100, 169, 253, 264, 316
BssT1I CCWWGG 3 cut(s) 100, 169, 264
Bst4CI ACNGT 3 cut(s) 145, 517, 540
Bst6I CTCTTC 1 cut(s) 26
BstC8I GCNNGC 2 cut(s) 112, 180
BstDEI CTNAG 1 cut(s) 597
BstENI CCTNNNNNAGG 1 cut(s) 410
BstF5I GGATG 2 cut(s) 180, 303
BstMWI GCNNNNNNNGC 5 cut(s) 89, 111, 120, 249, 507
BstNSI RCATGY 1 cut(s) 65
BstSCI CCNGG 2 cut(s) 315, 316
BstV1I GCAGC 2 cut(s) 104, 580
BsuRI GGCC 5 cut(s) 105, 168, 243, 252, 270
BtrI CACGTC 1 cut(s) 280
BtsCI GGATG 2 cut(s) 180, 303
BtsI GCAGTG 1 cut(s) 508
BtsIMutI CAGTG 2 cut(s) 508, 536
Cac8I GCNNGC 2 cut(s) 112, 180
Cfr13I GGNCC 3 cut(s) 104, 166, 268
Cfr9I CCCGGG 1 cut(s) 316
CseI GACGC 1 cut(s) 599
Csp6I GTAC 2 cut(s) 199, 630
CviAII CATG 3 cut(s) 45, 62, 120
CviQI GTAC 2 cut(s) 199, 630
DdeI CTNAG 1 cut(s) 597
DraI TTTAAA 1 cut(s) 544
Eam1104I CTCTTC 1 cut(s) 26
EarI CTCTTC 1 cut(s) 26
Eco130I CCWWGG 3 cut(s) 100, 169, 264
Eco32I GATATC 1 cut(s) 565
Eco88I CYCGRG 2 cut(s) 163, 316
EcoNI CCTNNNNNAGG 1 cut(s) 410
EcoO109I RGGNCCY 1 cut(s) 104
EcoRV GATATC 1 cut(s) 565
EcoT14I CCWWGG 3 cut(s) 100, 169, 264
ErhI CCWWGG 3 cut(s) 100, 169, 264
FaeI CATG 3 cut(s) 48, 65, 123
FaiI YATR 8 cut(s) 46, 63, 121, 425, 485, 487, 489, 569
FaqI GGGAC 1 cut(s) 284
FatI CATG 3 cut(s) 44, 61, 119
FauI CCCGC 2 cut(s) 115, 359
Fnu4HI GCNGC 2 cut(s) 93, 594
FokI GGATG 2 cut(s) 187, 290
Fsp4HI GCNGC 2 cut(s) 93, 594
GluI GCNGC 2 cut(s) 93, 594
GsuI CTGGAG 1 cut(s) 459
HaeIII GGCC 5 cut(s) 105, 168, 243, 252, 270
HapII CCGG 3 cut(s) 240, 271, 317
HgaI GACGC 1 cut(s) 599
Hin1II CATG 3 cut(s) 48, 65, 123
HinfI GANTC 3 cut(s) 210, 460, 601
HpaII CCGG 3 cut(s) 240, 271, 317
HphI GGTGA 2 cut(s) 305, 428
Hpy166II GTNNAC 1 cut(s) 160
Hpy188I TCNGA 2 cut(s) 600, 606
Hpy8I GTNNAC 1 cut(s) 160
HpyAV CCTTC 1 cut(s) 341
HpyCH4III ACNGT 3 cut(s) 145, 517, 540
HpyCH4IV ACGT 1 cut(s) 279
HpyCH4V TGCA 5 cut(s) 83, 123, 134, 221, 520
HpyF10VI GCNNNNNNNGC 5 cut(s) 89, 111, 120, 249, 507
HpyF3I CTNAG 1 cut(s) 597
HpySE526I ACGT 1 cut(s) 279
Hsp92II CATG 3 cut(s) 48, 65, 123
LmnI GCTCC 2 cut(s) 100, 320
Lsp1109I GCAGC 2 cut(s) 104, 580
LweI GCATC 1 cut(s) 165
MaeII ACGT 1 cut(s) 279
MaeIII GTNAC 2 cut(s) 139, 416
MboII GAAGA 2 cut(s) 13, 422
MluCI AATT 2 cut(s) 360, 634
MnlI CCTC 7 cut(s) 29, 61, 254, 263, 337, 388, 444
MseI TTAA 3 cut(s) 231, 543, 637
MspA1I CMGCKG 1 cut(s) 479
MspI CCGG 3 cut(s) 240, 271, 317
MspR9I CCNGG 2 cut(s) 317, 318
MwoI GCNNNNNNNGC 5 cut(s) 89, 111, 120, 249, 507
NciI CCSGG 2 cut(s) 317, 318
NlaIII CATG 3 cut(s) 48, 65, 123
NlaIV GGNNCC 1 cut(s) 106
NmeAIII GCCGAG 1 cut(s) 234
NmuCI GTSAC 1 cut(s) 416
NspI RCATGY 1 cut(s) 65
PfeI GAWTC 3 cut(s) 210, 460, 601
PkrI GCNGC 2 cut(s) 94, 595
PspN4I GGNNCC 1 cut(s) 106
PspPI GGNCC 3 cut(s) 104, 166, 268
RsaI GTAC 2 cut(s) 200, 631
RsaNI GTAC 2 cut(s) 199, 630
SaqAI TTAA 3 cut(s) 231, 543, 637
SatI GCNGC 2 cut(s) 93, 594
Sau96I GGNCC 3 cut(s) 104, 166, 268
ScrFI CCNGG 2 cut(s) 317, 318
SetI ASST 6 cut(s) 97, 141, 200, 282, 325, 393
SfaNI GCATC 1 cut(s) 165
SfiI GGCCNNNNNGGCC 1 cut(s) 249
SmaI CCCGGG 1 cut(s) 318
SmlI CTYRAG 1 cut(s) 435
SmoI CTYRAG 1 cut(s) 435
Sse9I AATT 2 cut(s) 360, 634
SsiI CCGC 3 cut(s) 108, 366, 479
StyD4I CCNGG 2 cut(s) 315, 316
StyI CCWWGG 3 cut(s) 100, 169, 264
TaaI ACNGT 3 cut(s) 145, 517, 540
TaiI ACGT 1 cut(s) 282
TaqI TCGA 2 cut(s) 356, 380
TasI AATT 2 cut(s) 360, 634
TfiI GAWTC 3 cut(s) 210, 460, 601
Tru1I TTAA 3 cut(s) 231, 543, 637
Tru9I TTAA 3 cut(s) 231, 543, 637
TscAI CASTG 2 cut(s) 508, 543
TseFI GTSAC 1 cut(s) 416
TseI GCWGC 2 cut(s) 92, 593
Tsp45I GTSAC 1 cut(s) 416
TspDTI ATGAA 3 cut(s) 25, 33, 361
TspMI CCCGGG 1 cut(s) 316
TspRI CASTG 2 cut(s) 508, 543
XagI CCTNNNNNAGG 1 cut(s) 410
XceI RCATGY 1 cut(s) 65
XcmI CCANNNNNNNNNTGG 1 cut(s) 167
XmaI CCCGGG 1 cut(s) 316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.