Rroxscaffold_5G00351190

OTU-like cysteine protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0015699
Physical Location & Seq
Forward (+)
27188556 .. 27199443
10888 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00351190.1

Sequence Viewer

Length: 636 bp
ATGGGAATAATTATAGGTCGTGGGAAAGACCACTTAGGTGATATTGAGGACCCACTTCCACAAAACAATCCTCAACAACTCAACGAGTTAAATCCGAGGTTCCCATCCCTCGAAGATCATAACTCGCGAAAGCGAACTTCTTCTAATACGAACCTACAAGACGATCTTTACGGTTCTAAGAGCGGCGATTCGCCACTTCGCAGGAGAAACGAAGACGCGGATGAGGGGACTTCACACGAGAGCTTGAGTGAGGAACGGCTTCTCTTTGGTCGAAGGTGCTTTGGAAGAAGACAGATGAGGGCTTGTGGAGCCGGCATACGCGGCGTAGTAGGTTCGCCGGAGGGCTTAACAAAAGATGTTTCAAAGAAGAAGAAGAAAAATAAGAGCAAGAAAAAGAAAGCACGGGTAGAACATTTGCAAGCATCTGTCTTCCCACAAGATTGGTCACAAGCAATCTATTGGAAATTATGGCTCGGGACGGGAGAGTGTTCGGGCGAGGCGGATGAAGTTGGGCCTTCTACTTCGTGTTCTAGCCCGGATGAAAAGTCGTACTATGTAGATGAGTGGTCATATTCCCTAGAGCTGGCTAATGATTATACATTTGATTGGAGGAGTACTGCTGAAACAATTGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OTU

Protein Analysis

211

Amino Acids

23.74

Weight (kDa)

6.45

Isoelectric Point (pI)

59.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 183
AccII CGCG 3 cut(s) 127, 218, 321
AciI CCGC 4 cut(s) 183, 218, 321, 500
AfaI GTAC 2 cut(s) 551, 616
AfiI CCNNNNNNNGG 1 cut(s) 583
AgsI TTSAA 1 cut(s) 363
AleI CACNNNNGTG 1 cut(s) 36
AluBI AGCT 2 cut(s) 243, 583
AluI AGCT 2 cut(s) 243, 583
Ama87I CYCGRG 1 cut(s) 473
AoxI GGCC 1 cut(s) 512
Asp700I GAANNNNTTC 2 cut(s) 139, 258
AspS9I GGNCC 2 cut(s) 49, 512
AsuC2I CCSGG 1 cut(s) 536
AsuHPI GGTGA 1 cut(s) 50
AvaI CYCGRG 1 cut(s) 473
AvaII GGWCC 1 cut(s) 49
BauI CACGAG 1 cut(s) 236
BbsI GAAGAC 3 cut(s) 219, 295, 421
BccI CCATC 1 cut(s) 112
BceAI ACGGC 1 cut(s) 272
BcnI CCSGG 1 cut(s) 536
BfaI CTAG 2 cut(s) 531, 578
BisI GCNGC 2 cut(s) 184, 322
BlsI GCNGC 2 cut(s) 185, 323
BmcAI AGTACT 1 cut(s) 616
Bme1390I CCNGG 1 cut(s) 536
Bme18I GGWCC 1 cut(s) 49
BmeT110I CYCGRG 1 cut(s) 473
BmgT120I GGNCC 2 cut(s) 49, 512
BmiI GGNNCC 3 cut(s) 51, 101, 310
BmrFI CCNGG 1 cut(s) 536
BmsI GCATC 1 cut(s) 431
BpiI GAAGAC 3 cut(s) 219, 295, 421
BpuEI CTTGAG 1 cut(s) 265
BpuMI CCSGG 1 cut(s) 536
BsaJI CCNNGG 1 cut(s) 95
Bsc4I CCNNNNNNNGG 1 cut(s) 583
Bse118I RCCGGY 1 cut(s) 311
BseDI CCNNGG 1 cut(s) 95
BseGI GGATG 4 cut(s) 104, 226, 508, 544
BseLI CCNNNNNNNGG 1 cut(s) 583
BseRI GAGGAG 1 cut(s) 625
Bsh1236I CGCG 3 cut(s) 127, 218, 321
BshFI GGCC 1 cut(s) 514
BsiHKCI CYCGRG 1 cut(s) 473
BsiSI CCGG 3 cut(s) 312, 338, 536
BslFI GGGAC 2 cut(s) 241, 490
BslI CCNNNNNNNGG 1 cut(s) 583
BsmFI GGGAC 2 cut(s) 241, 490
BsnI GGCC 1 cut(s) 514
BsoBI CYCGRG 1 cut(s) 473
Bsp143I GATC 2 cut(s) 115, 163
Bsp68I TCGCGA 1 cut(s) 127
BspACI CCGC 4 cut(s) 183, 218, 321, 500
BspANI GGCC 1 cut(s) 514
BspFNI CGCG 3 cut(s) 127, 218, 321
BspLI GGNNCC 3 cut(s) 51, 101, 310
BsrBI CCGCTC 1 cut(s) 183
BsrFI RCCGGY 1 cut(s) 311
BssAI RCCGGY 1 cut(s) 311
BssECI CCNNGG 1 cut(s) 95
BssMI GATC 2 cut(s) 115, 163
BssSI CACGAG 1 cut(s) 236
Bst2BI CACGAG 1 cut(s) 236
Bst4CI ACNGT 1 cut(s) 173
BstC8I GCNNGC 3 cut(s) 313, 420, 585
BstDEI CTNAG 2 cut(s) 34, 177
BstF5I GGATG 4 cut(s) 104, 226, 508, 544
BstFNI CGCG 3 cut(s) 127, 218, 321
BstKTI GATC 2 cut(s) 118, 166
BstMBI GATC 2 cut(s) 115, 163
BstMWI GCNNNNNNNGC 2 cut(s) 308, 321
BstSCI CCNGG 1 cut(s) 534
BstUI CGCG 3 cut(s) 127, 218, 321
BstV2I GAAGAC 3 cut(s) 219, 295, 421
BstXI CCANNNNNNTGG 1 cut(s) 441
BsuRI GGCC 1 cut(s) 514
BtsCI GGATG 4 cut(s) 104, 226, 508, 544
BtuMI TCGCGA 1 cut(s) 127
Cac8I GCNNGC 3 cut(s) 313, 420, 585
Cfr10I RCCGGY 1 cut(s) 311
Cfr13I GGNCC 2 cut(s) 49, 512
CseI GACGC 1 cut(s) 224
Csp6I GTAC 2 cut(s) 550, 615
CviQI GTAC 2 cut(s) 550, 615
DdeI CTNAG 2 cut(s) 34, 177
DpnI GATC 2 cut(s) 117, 165
DpnII GATC 2 cut(s) 115, 163
EciI GGCGGA 1 cut(s) 515
Eco47I GGWCC 1 cut(s) 49
Eco88I CYCGRG 1 cut(s) 473
EcoO109I RGGNCCY 1 cut(s) 49
FaiI YATR 7 cut(s) 14, 120, 317, 469, 555, 571, 597
FalI AAGNNNNNCTT 2 cut(s) 150, 182
FaqI GGGAC 2 cut(s) 241, 490
Fnu4HI GCNGC 2 cut(s) 184, 322
FokI GGATG 4 cut(s) 91, 233, 515, 551
Fsp4HI GCNGC 2 cut(s) 184, 322
FspBI CTAG 2 cut(s) 531, 578
GluI GCNGC 2 cut(s) 184, 322
HaeIII GGCC 1 cut(s) 514
HapII CCGG 3 cut(s) 312, 338, 536
HgaI GACGC 1 cut(s) 224
HinfI GANTC 1 cut(s) 188
HpaII CCGG 3 cut(s) 312, 338, 536
HphI GGTGA 1 cut(s) 50
Hpy188I TCNGA 1 cut(s) 96
Hpy188III TCNNGA 2 cut(s) 126, 475
HpyAV CCTTC 2 cut(s) 267, 525
HpyCH4III ACNGT 1 cut(s) 173
HpyCH4V TGCA 1 cut(s) 418
HpyF10VI GCNNNNNNNGC 2 cut(s) 308, 321
HpyF3I CTNAG 2 cut(s) 34, 177
KroI GCCGGC 1 cut(s) 311
KroNI GCCGGC 1 cut(s) 313
Kzo9I GATC 2 cut(s) 115, 163
LmnI GCTCC 1 cut(s) 308
LpnPI CCDG 5 cut(s) 187, 325, 351, 549, 569
LweI GCATC 1 cut(s) 431
MaeI CTAG 2 cut(s) 531, 578
MaeIII GTNAC 1 cut(s) 444
MalI GATC 2 cut(s) 117, 165
MbiI CCGCTC 1 cut(s) 183
MboI GATC 2 cut(s) 115, 163
MboII GAAGA 9 cut(s) 125, 132, 224, 297, 300, 379, 382, 385, 421
MfeI CAATTG 1 cut(s) 627
MluCI AATT 3 cut(s) 9, 464, 627
MroNI GCCGGC 1 cut(s) 311
MroXI GAANNNNTTC 2 cut(s) 139, 258
MseI TTAA 2 cut(s) 89, 347
MslI CAYNNNNRTG 1 cut(s) 36
MspI CCGG 3 cut(s) 312, 338, 536
MspR9I CCNGG 1 cut(s) 536
MunI CAATTG 1 cut(s) 627
MvnI CGCG 3 cut(s) 127, 218, 321
MwoI GCNNNNNNNGC 2 cut(s) 308, 321
NaeI GCCGGC 1 cut(s) 313
NciI CCSGG 1 cut(s) 536
NdeII GATC 2 cut(s) 115, 163
NgoMIV GCCGGC 1 cut(s) 311
NlaIV GGNNCC 3 cut(s) 51, 101, 310
NmuCI GTSAC 1 cut(s) 444
NruI TCGCGA 1 cut(s) 127
OliI CACNNNNGTG 1 cut(s) 36
PdiI GCCGGC 1 cut(s) 313
PdmI GAANNNNTTC 2 cut(s) 139, 258
PfeI GAWTC 1 cut(s) 188
PkrI GCNGC 2 cut(s) 185, 323
PpuMI RGGWCCY 1 cut(s) 49
Psp5II RGGWCCY 1 cut(s) 49
PspN4I GGNNCC 3 cut(s) 51, 101, 310
PspPI GGNCC 2 cut(s) 49, 512
PspPPI RGGWCCY 1 cut(s) 49
RruI TCGCGA 1 cut(s) 127
RsaI GTAC 2 cut(s) 551, 616
RsaNI GTAC 2 cut(s) 550, 615
RseI CAYNNNNRTG 1 cut(s) 36
SaqAI TTAA 2 cut(s) 89, 347
SatI GCNGC 2 cut(s) 184, 322
Sau3AI GATC 2 cut(s) 115, 163
Sau96I GGNCC 2 cut(s) 49, 512
ScaI AGTACT 1 cut(s) 616
ScrFI CCNGG 1 cut(s) 536
SetI ASST 8 cut(s) 19, 40, 101, 156, 245, 278, 334, 585
SfaNI GCATC 1 cut(s) 431
SinI GGWCC 1 cut(s) 49
SmiMI CAYNNNNRTG 1 cut(s) 36
SmlI CTYRAG 1 cut(s) 244
SmoI CTYRAG 1 cut(s) 244
Sse9I AATT 3 cut(s) 9, 464, 627
SsiI CCGC 4 cut(s) 183, 218, 321, 500
SspMI CTAG 2 cut(s) 531, 578
StyD4I CCNGG 1 cut(s) 534
TaaI ACNGT 1 cut(s) 173
TaqI TCGA 2 cut(s) 111, 271
TasI AATT 3 cut(s) 9, 464, 627
TatI WGTACW 1 cut(s) 614
TauI GCSGC 2 cut(s) 186, 324
TfiI GAWTC 1 cut(s) 188
Tru1I TTAA 2 cut(s) 89, 347
Tru9I TTAA 2 cut(s) 89, 347
TseFI GTSAC 1 cut(s) 444
Tsp45I GTSAC 1 cut(s) 444
TspDTI ATGAA 2 cut(s) 519, 555
VpaK11BI GGWCC 1 cut(s) 49
XmnI GAANNNNTTC 2 cut(s) 139, 258
XspI CTAG 2 cut(s) 531, 578
ZrmI AGTACT 1 cut(s) 616
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.