Rroxscaffold_7G00214650

Proline iminopeptidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0007440
Physical Location & Seq
Reverse (-)
65301497 .. 65307540
6044 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00214650.1

Sequence Viewer

Length: 1458 bp
ATGACTATGTTAGTGACCTTGGTATTCTCCCCAAATTCCTTCTCTTCCGCACCCTCCCTTCCCTTAATCTCCAACACCTCCTCCCCTTCTTCCTTCTCCTTCTCCTCCAAGCTCACTGCTTCAGGGAGGCGTAATTTGGTTCTACGTGGTTGCAAGACTGAGACGAAGCTACGAGATTCGATGGAATCGGAGAAGGAATTGCCGGAATTCAACAGAGAACTGTATCCGAGCATTGAGCCTTACAGCTCCGGCTTTCTCAAGGTTTCCGATATTCACTCAATCTATTGGGAGCAGTCCGGAAATCCCAACGGCCATGCTACTGTAAAATCTATATATTGCTGTCAATGTAGGATGGTTGATGCAGTTTGTCAACTCAGCTTTAGTTTAGAGTTAGAACTGGCAGTCTCTTCTGCACTCTTAGCAGTAGAAGAACTCACCAAATGTTTGGCAAAAGATGCAGAACCACAAGTAAGCATTAGAAACAACTTGGATGGCTGTTCTTTAAATGAGATGTGTTGTACTAGGGCTCACACTGTACCTGTTATATTTCTTCATGGGGGTCCAGGAGGTGGAACTTCCCCAAATAATCGAAGATTCTTTGATCCTGAGTTTTACAGAATCATCCTAGTGGATCAGCGAGGTTCGGGGAAAAGTACTCCACATGCTTGCTTGGAAGAAAACACCACATGGGATCTCATTGGTGACATTGAAAAGCTTAGAGAACACCTGGAAATTCCAGAATGGCAGGTTTTTGGTGGATCTTGGGGAGTACACTTGCCCTTGCATATAGCCAATCACACCCTGAGAAGAAAGAAAGAGATTGATTGGTTTTATGAGGGTGGTGCTGCTGCCATATACCCTGATGCTTGGGAGCAATTTCGTGATCTGATTCCGGAAAATGAGCGGGGAAGTTTTGTTGATGCTTATAGGAAGAGATTAAACTCCGATGATAAGGAAATACAAGTTGTTAGTGCACCTTCGCATCCCTTTAAAGGACCTTTTACATGGAATGTAAATATTGATTTCACAGTTGCACACATCGCCATCACAGTTTCCAGAAACCAGCATCCTCTTGTATATCTAGCAGCTGCTAGAGCATGGACAATGTGGGAAATGATGACTGCACATCTCCTTCCAAATGACGAGAACATCAAGAGGGGGAAGACGATATTTTTGCATTGGATTGAAAATCATTACTTTGTGAACAGAGGATTCTTTCCTTCAGATGCATACCTGTTAGATAACGTTGAAAAGATAAGGCATATAAAAACTACAATCGTACAGGGACGATATGATGTTTGCTGTCCCATGATGTCGGCGTGGGATCTCCATAAAGCATGGCCAGAAGCTGATCTCAAGGTAATTCAAGATGCAGGACATTCTGCTAATGAACCAGGAATAGCTGCAGAGCTCGTGGCGGCAAATGAGAAGCTGAAACACATAAAAATTGGAGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00330 map00330
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

485

Amino Acids

54.67

Weight (kDa)

6.1

Isoelectric Point (pI)

43.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 180 - 256 1.8e-13 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 736
AccB7I CCANNNNNTGG 1 cut(s) 569
AccBSI CCGCTC 1 cut(s) 904
AccIII TCCGGA 2 cut(s) 296, 892
AciI CCGC 3 cut(s) 48, 904, 1418
AclI AACGTT 1 cut(s) 1245
AclWI GGATC 5 cut(s) 596, 639, 699, 766, 1332
AcoI YGGCCR 2 cut(s) 310, 1340
AcsI RAATTY 3 cut(s) 34, 206, 732
AcuI CTGAAG 2 cut(s) 105, 1206
AfaI GTAC 5 cut(s) 520, 537, 655, 771, 1281
AfiI CCNNNNNNNGG 2 cut(s) 569, 992
AgsI TTSAA 5 cut(s) 211, 710, 1187, 1250, 1367
AjnI CCWGG 3 cut(s) 562, 726, 1393
Alw21I GWGCWC 2 cut(s) 976, 1413
Alw26I GTCTC 2 cut(s) 155, 409
Alw44I GTGCAC 1 cut(s) 972
AlwI GGATC 5 cut(s) 596, 639, 699, 766, 1332
AlwNI CAGNNNCTG 1 cut(s) 1349
Aor13HI TCCGGA 2 cut(s) 296, 892
AoxI GGCC 2 cut(s) 310, 1340
ApaLI GTGCAC 1 cut(s) 972
ApeKI GCWGC 5 cut(s) 845, 848, 1085, 1088, 1403
ApoI RAATTY 3 cut(s) 34, 206, 732
ArsI GACNNNNNNTTYG 2 cut(s) 1156, 1188
AspS9I GGNCC 2 cut(s) 560, 995
AsuHPI GGTGA 2 cut(s) 427, 713
AvaII GGWCC 2 cut(s) 560, 995
BaeGI GKGCMC 1 cut(s) 976
BalI TGGCCA 1 cut(s) 1342
BanII GRGCYC 2 cut(s) 529, 1413
BauI CACGAG 1 cut(s) 1412
BbsI GAAGAC 1 cut(s) 1169
Bbv12I GWGCWC 2 cut(s) 976, 1413
BbvI GCAGC 5 cut(s) 832, 835, 1075, 1097, 1390
BccI CCATC 4 cut(s) 175, 346, 485, 1052
BceAI ACGGC 1 cut(s) 325
BcgI CGANNNNNNTGC 2 cut(s) 1156, 1190
BciT130I CCWGG 3 cut(s) 564, 728, 1395
BciVI GTATCC 1 cut(s) 234
BcoDI GTCTC 2 cut(s) 155, 409
BfaI CTAG 4 cut(s) 522, 626, 1082, 1092
BfmI CTRYAG 1 cut(s) 1404
BfuAI ACCTGC 1 cut(s) 736
BfuI GTATCC 1 cut(s) 234
BisI GCNGC 6 cut(s) 846, 849, 1086, 1089, 1404, 1419
BlsI GCNGC 6 cut(s) 847, 850, 1087, 1090, 1405, 1420
BmcAI AGTACT 1 cut(s) 655
Bme1390I CCNGG 3 cut(s) 564, 728, 1395
Bme18I GGWCC 2 cut(s) 560, 995
BmgT120I GGNCC 2 cut(s) 560, 995
BmiI GGNNCC 1 cut(s) 561
BmrFI CCNGG 3 cut(s) 564, 728, 1395
BmsI GCATC 8 cut(s) 349, 445, 853, 910, 991, 1075, 1216, 1360
BpiI GAAGAC 1 cut(s) 1169
BpuEI CTTGAG 2 cut(s) 242, 1340
BsaAI YACGTR 1 cut(s) 146
BsaJI CCNNGG 1 cut(s) 18
BsaWI WCCGGW 2 cut(s) 296, 892
BsaXI ACNNNNNCTCC 2 cut(s) 65, 95
Bsc4I CCNNNNNNNGG 2 cut(s) 569, 992
Bse1I ACTGG 1 cut(s) 402
BseAI TCCGGA 2 cut(s) 296, 892
BseBI CCWGG 3 cut(s) 564, 728, 1395
BseDI CCNNGG 1 cut(s) 18
BseGI GGATG 5 cut(s) 357, 496, 621, 982, 1066
BseLI CCNNNNNNNGG 2 cut(s) 569, 992
BseMII CTCAG 4 cut(s) 150, 388, 597, 794
BseNI ACTGG 1 cut(s) 402
BseRI GAGGAG 2 cut(s) 70, 94
BseSI GKGCMC 1 cut(s) 976
BseXI GCAGC 5 cut(s) 832, 835, 1075, 1097, 1390
BsgI GTGCAG 2 cut(s) 396, 1107
BshFI GGCC 2 cut(s) 312, 1342
BsiHKAI GWGCWC 2 cut(s) 976, 1413
BsiSI CCGG 4 cut(s) 203, 249, 297, 893
BslFI GGGAC 2 cut(s) 1290, 1299
BslI CCNNNNNNNGG 2 cut(s) 569, 992
BsmAI GTCTC 2 cut(s) 155, 409
BsmBI CGTCTC 1 cut(s) 155
BsmFI GGGAC 2 cut(s) 1290, 1299
BsnI GGCC 2 cut(s) 312, 1342
Bsp1286I GDGCHC 3 cut(s) 529, 976, 1413
Bsp13I TCCGGA 2 cut(s) 296, 892
Bsp143I GATC 7 cut(s) 601, 631, 691, 758, 883, 1324, 1351
BspACI CCGC 3 cut(s) 48, 904, 1418
BspANI GGCC 2 cut(s) 312, 1342
BspCNI CTCAG 4 cut(s) 151, 387, 598, 795
BspEI TCCGGA 2 cut(s) 296, 892
BspLI GGNNCC 1 cut(s) 561
BspMAI CTGCAG 1 cut(s) 1408
BspMI ACCTGC 1 cut(s) 736
BspPI GGATC 5 cut(s) 596, 639, 699, 766, 1332
BsrBI CCGCTC 1 cut(s) 904
BsrI ACTGG 1 cut(s) 402
BssECI CCNNGG 1 cut(s) 18
BssMI GATC 7 cut(s) 601, 631, 691, 758, 883, 1324, 1351
BssSI CACGAG 1 cut(s) 1412
BssT1I CCWWGG 1 cut(s) 18
Bst2BI CACGAG 1 cut(s) 1412
Bst2UI CCWGG 3 cut(s) 564, 728, 1395
Bst4CI ACNGT 5 cut(s) 222, 322, 535, 1030, 1051
Bst6I CTCTTC 3 cut(s) 49, 412, 926
BstAPI GCANNNNNTGC 1 cut(s) 455
BstBAI YACGTR 1 cut(s) 146
BstC8I GCNNGC 1 cut(s) 667
BstDEI CTNAG 6 cut(s) 159, 374, 418, 606, 716, 803
BstF5I GGATG 5 cut(s) 357, 496, 621, 982, 1066
BstKTI GATC 7 cut(s) 604, 634, 694, 761, 886, 1327, 1354
BstMAI GTCTC 2 cut(s) 155, 409
BstMBI GATC 7 cut(s) 601, 631, 691, 758, 883, 1324, 1351
BstMWI GCNNNNNNNGC 4 cut(s) 419, 455, 1040, 1094
BstNI CCWGG 3 cut(s) 564, 728, 1395
BstNSI RCATGY 1 cut(s) 665
BstSCI CCNGG 3 cut(s) 562, 726, 1393
BstSFI CTRYAG 1 cut(s) 1404
BstSLI GKGCMC 1 cut(s) 976
BstV1I GCAGC 5 cut(s) 832, 835, 1075, 1097, 1390
BstV2I GAAGAC 1 cut(s) 1169
BstX2I RGATCY 3 cut(s) 691, 758, 1324
BstXI CCANNNNNNTGG 1 cut(s) 445
BstYI RGATCY 3 cut(s) 691, 758, 1324
BsuI GTATCC 1 cut(s) 234
BsuRI GGCC 2 cut(s) 312, 1342
BtgZI GCGATG 1 cut(s) 1024
BtsCI GGATG 5 cut(s) 357, 496, 621, 982, 1066
BtsI GCAGTG 1 cut(s) 114
BtsIMutI CAGTG 2 cut(s) 114, 531
BveI ACCTGC 1 cut(s) 736
Cac8I GCNNGC 1 cut(s) 667
CaiI CAGNNNCTG 1 cut(s) 1349
Cfr13I GGNCC 2 cut(s) 560, 995
Csp6I GTAC 5 cut(s) 519, 536, 654, 770, 1280
CviAII CATG 8 cut(s) 314, 554, 662, 687, 1005, 1098, 1309, 1338
CviQI GTAC 5 cut(s) 519, 536, 654, 770, 1280
DdeI CTNAG 6 cut(s) 159, 374, 418, 606, 716, 803
DpnI GATC 7 cut(s) 603, 633, 693, 760, 885, 1326, 1353
DpnII GATC 7 cut(s) 601, 631, 691, 758, 883, 1324, 1351
DraI TTTAAA 2 cut(s) 504, 991
EaeI YGGCCR 2 cut(s) 310, 1340
Eam1104I CTCTTC 3 cut(s) 49, 412, 926
EarI CTCTTC 3 cut(s) 49, 412, 926
Ecl136II GAGCTC 1 cut(s) 1411
Eco130I CCWWGG 1 cut(s) 18
Eco24I GRGCYC 2 cut(s) 529, 1413
Eco47I GGWCC 2 cut(s) 560, 995
Eco53kI GAGCTC 1 cut(s) 1411
Eco57I CTGAAG 2 cut(s) 105, 1206
EcoICRI GAGCTC 1 cut(s) 1411
EcoO109I RGGNCCY 1 cut(s) 995
EcoRI GAATTC 1 cut(s) 206
EcoRII CCWGG 3 cut(s) 562, 726, 1393
EcoT14I CCWWGG 1 cut(s) 18
EcoT22I ATGCAT 1 cut(s) 1231
EcoT38I GRGCYC 2 cut(s) 529, 1413
ErhI CCWWGG 1 cut(s) 18
Esp3I CGTCTC 1 cut(s) 155
FaeI CATG 8 cut(s) 317, 557, 665, 690, 1008, 1101, 1312, 1341
FaqI GGGAC 2 cut(s) 1290, 1299
FatI CATG 8 cut(s) 313, 553, 661, 686, 1004, 1097, 1308, 1337
FauI CCCGC 1 cut(s) 897
Fnu4HI GCNGC 6 cut(s) 846, 849, 1086, 1089, 1404, 1419
FokI GGATG 5 cut(s) 364, 503, 608, 969, 1053
FriOI GRGCYC 2 cut(s) 529, 1413
Fsp4HI GCNGC 6 cut(s) 846, 849, 1086, 1089, 1404, 1419
FspBI CTAG 4 cut(s) 522, 626, 1082, 1092
GluI GCNGC 6 cut(s) 846, 849, 1086, 1089, 1404, 1419
HaeIII GGCC 2 cut(s) 312, 1342
HapII CCGG 4 cut(s) 203, 249, 297, 893
Hin1II CATG 8 cut(s) 317, 557, 665, 690, 1008, 1101, 1312, 1341
HincII GTYRAC 1 cut(s) 371
HindII GTYRAC 1 cut(s) 371
HindIII AAGCTT 1 cut(s) 713
HinfI GANTC 6 cut(s) 176, 185, 594, 618, 889, 1212
HpaII CCGG 4 cut(s) 203, 249, 297, 893
HphI GGTGA 2 cut(s) 427, 713
Hpy166II GTNNAC 4 cut(s) 371, 772, 974, 1204
Hpy188I TCNGA 6 cut(s) 190, 228, 268, 888, 946, 1225
Hpy188III TCNNGA 8 cut(s) 297, 605, 737, 881, 893, 1056, 1153, 1367
Hpy8I GTNNAC 4 cut(s) 371, 772, 974, 1204
HpyAV CCTTC 9 cut(s) 49, 68, 96, 103, 109, 187, 987, 1142, 1230
HpyCH4III ACNGT 5 cut(s) 222, 322, 535, 1030, 1051
HpyCH4IV ACGT 2 cut(s) 145, 1245
HpyF10VI GCNNNNNNNGC 4 cut(s) 419, 455, 1040, 1094
HpyF3I CTNAG 6 cut(s) 159, 374, 418, 606, 716, 803
HpySE526I ACGT 2 cut(s) 145, 1245
Hsp92II CATG 8 cut(s) 317, 557, 665, 690, 1008, 1101, 1312, 1341
Kpn2I TCCGGA 2 cut(s) 296, 892
Kzo9I GATC 7 cut(s) 601, 631, 691, 758, 883, 1324, 1351
LmnI GCTCC 4 cut(s) 251, 289, 871, 1451
Lsp1109I GCAGC 5 cut(s) 832, 835, 1075, 1097, 1390
LweI GCATC 8 cut(s) 349, 445, 853, 910, 991, 1075, 1216, 1360
MaeI CTAG 4 cut(s) 522, 626, 1082, 1092
MaeII ACGT 2 cut(s) 145, 1245
MaeIII GTNAC 2 cut(s) 13, 701
MalI GATC 7 cut(s) 603, 633, 693, 760, 885, 1326, 1353
MbiI CCGCTC 1 cut(s) 904
MboI GATC 7 cut(s) 601, 631, 691, 758, 883, 1324, 1351
MflI RGATCY 3 cut(s) 691, 758, 1324
MhlI GDGCHC 3 cut(s) 529, 976, 1413
MlsI TGGCCA 1 cut(s) 1342
MluCI AATT 8 cut(s) 34, 133, 197, 206, 732, 875, 1362, 1446
MluNI TGGCCA 1 cut(s) 1342
MmeI TCCRAC 1 cut(s) 96
Mox20I TGGCCA 1 cut(s) 1342
Mph1103I ATGCAT 1 cut(s) 1231
MroI TCCGGA 2 cut(s) 296, 892
MscI TGGCCA 1 cut(s) 1342
MseI TTAA 4 cut(s) 65, 503, 938, 990
MslI CAYNNNNRTG 1 cut(s) 626
Msp20I TGGCCA 1 cut(s) 1342
MspA1I CMGCKG 1 cut(s) 1088
MspI CCGG 4 cut(s) 203, 249, 297, 893
MspR9I CCNGG 3 cut(s) 564, 728, 1395
MvaI CCWGG 3 cut(s) 564, 728, 1395
MwoI GCNNNNNNNGC 4 cut(s) 419, 455, 1040, 1094
NdeII GATC 7 cut(s) 601, 631, 691, 758, 883, 1324, 1351
NlaIII CATG 8 cut(s) 317, 557, 665, 690, 1008, 1101, 1312, 1341
NlaIV GGNNCC 1 cut(s) 561
NmuCI GTSAC 2 cut(s) 13, 701
NsiI ATGCAT 1 cut(s) 1231
NspI RCATGY 1 cut(s) 665
PfeI GAWTC 6 cut(s) 176, 185, 594, 618, 889, 1212
PflMI CCANNNNNTGG 1 cut(s) 569
PfoI TCCNGGA 1 cut(s) 562
PkrI GCNGC 6 cut(s) 847, 850, 1087, 1090, 1405, 1420
Ppu21I YACGTR 1 cut(s) 146
PpuMI RGGWCCY 1 cut(s) 995
Psp124BI GAGCTC 1 cut(s) 1413
Psp1406I AACGTT 1 cut(s) 1245
Psp5II RGGWCCY 1 cut(s) 995
Psp6I CCWGG 3 cut(s) 562, 726, 1393
PspGI CCWGG 3 cut(s) 562, 726, 1393
PspN4I GGNNCC 1 cut(s) 561
PspPI GGNCC 2 cut(s) 560, 995
PspPPI RGGWCCY 1 cut(s) 995
PstI CTGCAG 1 cut(s) 1408
PstNI CAGNNNCTG 1 cut(s) 1349
PsuI RGATCY 3 cut(s) 691, 758, 1324
PvuII CAGCTG 1 cut(s) 1088
RsaI GTAC 5 cut(s) 520, 537, 655, 771, 1281
RsaNI GTAC 5 cut(s) 519, 536, 654, 770, 1280
RseI CAYNNNNRTG 1 cut(s) 626
SacI GAGCTC 1 cut(s) 1413
SaqAI TTAA 4 cut(s) 65, 503, 938, 990
SatI GCNGC 6 cut(s) 846, 849, 1086, 1089, 1404, 1419
Sau3AI GATC 7 cut(s) 601, 631, 691, 758, 883, 1324, 1351
Sau96I GGNCC 2 cut(s) 560, 995
ScaI AGTACT 1 cut(s) 655
ScrFI CCNGG 3 cut(s) 564, 728, 1395
SduI GDGCHC 3 cut(s) 529, 976, 1413
SfaNI GCATC 8 cut(s) 349, 445, 853, 910, 991, 1075, 1216, 1360
SfcI CTRYAG 1 cut(s) 1404
SinI GGWCC 2 cut(s) 560, 995
SmiMI CAYNNNNRTG 1 cut(s) 626
SmlI CTYRAG 2 cut(s) 257, 1355
SmoI CTYRAG 2 cut(s) 257, 1355
Sse9I AATT 8 cut(s) 34, 133, 197, 206, 732, 875, 1362, 1446
SsiI CCGC 3 cut(s) 48, 904, 1418
SspI AATATT 1 cut(s) 1018
SspMI CTAG 4 cut(s) 522, 626, 1082, 1092
SstI GAGCTC 1 cut(s) 1413
StyD4I CCNGG 3 cut(s) 562, 726, 1393
StyI CCWWGG 1 cut(s) 18
TaaI ACNGT 5 cut(s) 222, 322, 535, 1030, 1051
TaiI ACGT 2 cut(s) 148, 1248
TaqI TCGA 2 cut(s) 179, 589
TasI AATT 8 cut(s) 34, 133, 197, 206, 732, 875, 1362, 1446
TatI WGTACW 3 cut(s) 518, 653, 769
TauI GCSGC 1 cut(s) 1421
TfiI GAWTC 6 cut(s) 176, 185, 594, 618, 889, 1212
Tru1I TTAA 4 cut(s) 65, 503, 938, 990
Tru9I TTAA 4 cut(s) 65, 503, 938, 990
TscAI CASTG 2 cut(s) 121, 538
TseFI GTSAC 2 cut(s) 13, 701
TseI GCWGC 5 cut(s) 845, 848, 1085, 1088, 1403
Tsp45I GTSAC 2 cut(s) 13, 701
TspDTI ATGAA 2 cut(s) 542, 1404
TspRI CASTG 2 cut(s) 121, 538
Van91I CCANNNNNTGG 1 cut(s) 569
VneI GTGCAC 1 cut(s) 972
VpaK11BI GGWCC 2 cut(s) 560, 995
XapI RAATTY 3 cut(s) 34, 206, 732
XceI RCATGY 1 cut(s) 665
XspI CTAG 4 cut(s) 522, 626, 1082, 1092
ZrmI AGTACT 1 cut(s) 655
Zsp2I ATGCAT 1 cut(s) 1231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.