Rorug01G0343100

Protein YIPF1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0009035
Physical Location & Seq
Reverse (-)
46123656 .. 46127551
3896 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0343100.1

Sequence Viewer

Length: 567 bp
ATGGAGGTCCGTCCCAATCAGACGACGGACAACTCGTCGGCGCCGCCGCAGCGTAGCCAGCGCAATACCTCTCAGAAAAAGCCGCTGGCTTCTCCAATTCCGGTCGACTCCTCCTCCGTTTCTCAAAGGCTTCAGAAGGAGTTGATGTCTCTAATGATGAGTGGAGGAGAACTTGGAGTATCAGCTTTCCCTGAAGGTGACAGCATTTTTGCATGGATTGGCACAATTGAAGGTGGAAAAGGAACTATGTACGAGGGTTTATCTTACAAACTGTCCTTGAGATTTCCTATGGATTATCCTTTCAAGCCACCACTAGTCAAGTTTGAGACAATGTGCTTCCATCCAAATGTTGATCAGTTTGGCAACATATGCCTTGATATTCTGCAGGACAAGTGGTCTTCCGCTTATGATTGCAGGACTATTCTTCTGTCCATTCAAAGTCTGTTGGGAGAGCCAAACCCAGAGAGTCCCCTCAACAGCTATGCTGCTGCTCTTTGGAGTAACAAGGAAGATTACAGAAAAATGGTCCAGAAACAGTACTTTGCTGGAGAATCATTTGAGAGCTGA

Protein Analysis

188

Amino Acids

21.05

Weight (kDa)

5.25

Isoelectric Point (pI)

62.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 43 - 179 2.4e-44 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 40
AccI GTMKAC 1 cut(s) 105
AciI CCGC 4 cut(s) 44, 47, 83, 402
AcuI CTGAAG 2 cut(s) 116, 213
AcyI GRCGYC 1 cut(s) 41
AfaI GTAC 2 cut(s) 251, 539
AgsI TTSAA 3 cut(s) 230, 304, 437
AhdI GACNNNNNGTC 2 cut(s) 34, 394
AhlI ACTAGT 1 cut(s) 313
AluBI AGCT 3 cut(s) 185, 480, 564
AluI AGCT 3 cut(s) 185, 480, 564
Alw26I GTCTC 2 cut(s) 153, 320
ApeKI GCWGC 3 cut(s) 49, 485, 488
ArsI GACNNNNNNTTYG 2 cut(s) 191, 223
AspLEI GCGC 2 cut(s) 43, 63
AspS9I GGNCC 2 cut(s) 7, 526
AsuHPI GGTGA 1 cut(s) 209
AvaII GGWCC 2 cut(s) 7, 526
BanI GGYRCC 1 cut(s) 40
BbsI GAAGAC 1 cut(s) 390
BbvI GCAGC 3 cut(s) 61, 472, 475
BccI CCATC 1 cut(s) 348
BclI TGATCA 1 cut(s) 352
BcoDI GTCTC 2 cut(s) 153, 320
BcuI ACTAGT 1 cut(s) 313
BfaI CTAG 1 cut(s) 314
BfmI CTRYAG 1 cut(s) 383
BfoI RGCGCY 1 cut(s) 44
BisI GCNGC 6 cut(s) 44, 47, 50, 83, 486, 489
BlsI GCNGC 6 cut(s) 45, 48, 51, 84, 487, 490
BmcAI AGTACT 1 cut(s) 539
Bme18I GGWCC 2 cut(s) 7, 526
BmeRI GACNNNNNGTC 2 cut(s) 34, 394
BmgT120I GGNCC 2 cut(s) 7, 526
BmiI GGNNCC 1 cut(s) 42
BpiI GAAGAC 1 cut(s) 390
BpmI CTGGAG 1 cut(s) 567
BpuEI CTTGAG 1 cut(s) 298
BsaHI GRCGYC 1 cut(s) 41
BsaWI WCCGGW 1 cut(s) 100
BsaXI ACNNNNNCTCC 4 cut(s) 98, 128, 131, 161
BseGI GGATG 1 cut(s) 340
BseMII CTCAG 1 cut(s) 86
BseRI GAGGAG 3 cut(s) 100, 103, 180
BseXI GCAGC 3 cut(s) 61, 472, 475
Bsh1285I CGRYCG 1 cut(s) 105
BshNI GGYRCC 1 cut(s) 40
BsiEI CGRYCG 1 cut(s) 105
BsiSI CCGG 1 cut(s) 101
BslFI GGGAC 1 cut(s) 453
BsmAI GTCTC 2 cut(s) 153, 320
BsmFI GGGAC 1 cut(s) 453
Bsp143I GATC 1 cut(s) 352
BspACI CCGC 4 cut(s) 44, 47, 83, 402
BspCNI CTCAG 1 cut(s) 85
BspLI GGNNCC 1 cut(s) 42
BspMAI CTGCAG 1 cut(s) 387
BspT107I GGYRCC 1 cut(s) 40
BssMI GATC 1 cut(s) 352
BssNI GRCGYC 1 cut(s) 41
Bst4CI ACNGT 2 cut(s) 273, 537
BstACI GRCGYC 1 cut(s) 41
BstAPI GCANNNNNTGC 1 cut(s) 369
BstC8I GCNNGC 2 cut(s) 59, 87
BstDEI CTNAG 1 cut(s) 72
BstF5I GGATG 1 cut(s) 340
BstH2I RGCGCY 1 cut(s) 44
BstHHI GCGC 2 cut(s) 43, 63
BstKTI GATC 1 cut(s) 355
BstMAI GTCTC 2 cut(s) 153, 320
BstMBI GATC 1 cut(s) 352
BstMCI CGRYCG 1 cut(s) 105
BstMWI GCNNNNNNNGC 3 cut(s) 49, 58, 369
BstSFI CTRYAG 1 cut(s) 383
BstV1I GCAGC 3 cut(s) 61, 472, 475
BstV2I GAAGAC 1 cut(s) 390
BtsCI GGATG 1 cut(s) 340
Cac8I GCNNGC 2 cut(s) 59, 87
CfoI GCGC 2 cut(s) 43, 63
Cfr13I GGNCC 2 cut(s) 7, 526
Csp6I GTAC 2 cut(s) 250, 538
CviAII CATG 1 cut(s) 213
CviJI RGCY 9 cut(s) 57, 82, 89, 130, 185, 307, 454, 480, 564
CviKI_1 RGCY 9 cut(s) 57, 82, 89, 130, 185, 307, 454, 480, 564
CviQI GTAC 2 cut(s) 250, 538
DdeI CTNAG 1 cut(s) 72
DinI GGCGCC 1 cut(s) 42
DpnI GATC 1 cut(s) 354
DpnII GATC 1 cut(s) 352
DriI GACNNNNNGTC 2 cut(s) 34, 394
Eam1105I GACNNNNNGTC 2 cut(s) 34, 394
Eco47I GGWCC 2 cut(s) 7, 526
Eco57I CTGAAG 2 cut(s) 116, 213
EgeI GGCGCC 1 cut(s) 42
EheI GGCGCC 1 cut(s) 42
FaeI CATG 1 cut(s) 216
FaiI YATR 7 cut(s) 214, 248, 290, 368, 370, 408, 483
FaqI GGGAC 1 cut(s) 453
FatI CATG 1 cut(s) 212
FauNDI CATATG 1 cut(s) 368
FbaI TGATCA 1 cut(s) 352
FblI GTMKAC 1 cut(s) 105
Fnu4HI GCNGC 6 cut(s) 44, 47, 50, 83, 486, 489
FokI GGATG 1 cut(s) 327
Fsp4HI GCNGC 6 cut(s) 44, 47, 50, 83, 486, 489
FspBI CTAG 1 cut(s) 314
GlaI GCGC 2 cut(s) 42, 62
GluI GCNGC 6 cut(s) 44, 47, 50, 83, 486, 489
GsuI CTGGAG 1 cut(s) 567
HaeII RGCGCY 1 cut(s) 44
HapII CCGG 1 cut(s) 101
HhaI GCGC 2 cut(s) 43, 63
Hin1I GRCGYC 1 cut(s) 41
Hin1II CATG 1 cut(s) 216
Hin6I GCGC 2 cut(s) 41, 61
HinP1I GCGC 2 cut(s) 41, 61
HincII GTYRAC 1 cut(s) 106
HindII GTYRAC 1 cut(s) 106
HinfI GANTC 3 cut(s) 107, 466, 551
HpaII CCGG 1 cut(s) 101
HphI GGTGA 1 cut(s) 209
Hpy166II GTNNAC 1 cut(s) 106
Hpy188I TCNGA 3 cut(s) 21, 75, 135
Hpy188III TCNNGA 1 cut(s) 529
Hpy8I GTNNAC 1 cut(s) 106
Hpy99I CGWCG 2 cut(s) 28, 40
HpyAV CCTTC 3 cut(s) 130, 188, 224
HpyCH4III ACNGT 2 cut(s) 273, 537
HpyCH4V TGCA 3 cut(s) 212, 385, 414
HpyF10VI GCNNNNNNNGC 3 cut(s) 49, 58, 369
HpyF3I CTNAG 1 cut(s) 72
Hsp92I GRCGYC 1 cut(s) 41
Hsp92II CATG 1 cut(s) 216
HspAI GCGC 2 cut(s) 41, 61
KasI GGCGCC 1 cut(s) 40
Ksp22I TGATCA 1 cut(s) 352
Kzo9I GATC 1 cut(s) 352
LpnPI CCDG 9 cut(s) 71, 71, 114, 204, 371, 400, 474, 531, 542
Lsp1109I GCAGC 3 cut(s) 61, 472, 475
MaeI CTAG 1 cut(s) 314
MaeIII GTNAC 2 cut(s) 197, 500
MalI GATC 1 cut(s) 354
MboI GATC 1 cut(s) 352
MboII GAAGA 3 cut(s) 390, 416, 521
MfeI CAATTG 1 cut(s) 225
MluCI AATT 2 cut(s) 96, 225
Mly113I GGCGCC 1 cut(s) 41
MlyI GAGTC 2 cut(s) 101, 475
MnlI CCTC 6 cut(s) 79, 121, 124, 158, 247, 482
MslI CAYNNNNRTG 1 cut(s) 345
MspA1I CMGCKG 1 cut(s) 85
MspI CCGG 1 cut(s) 101
MunI CAATTG 1 cut(s) 225
MwoI GCNNNNNNNGC 3 cut(s) 49, 58, 369
NarI GGCGCC 1 cut(s) 41
NdeI CATATG 1 cut(s) 368
NdeII GATC 1 cut(s) 352
NlaIII CATG 1 cut(s) 216
NlaIV GGNNCC 1 cut(s) 42
NmuCI GTSAC 1 cut(s) 197
PcsI WCGNNNNNNNCGW 1 cut(s) 32
PfeI GAWTC 1 cut(s) 551
PkrI GCNGC 6 cut(s) 45, 48, 51, 84, 487, 490
PleI GAGTC 2 cut(s) 101, 474
PluTI GGCGCC 1 cut(s) 44
PpsI GAGTC 2 cut(s) 101, 474
PspN4I GGNNCC 1 cut(s) 42
PspPI GGNCC 2 cut(s) 7, 526
PstI CTGCAG 1 cut(s) 387
RsaI GTAC 2 cut(s) 251, 539
RsaNI GTAC 2 cut(s) 250, 538
RseI CAYNNNNRTG 1 cut(s) 345
SalI GTCGAC 1 cut(s) 104
SatI GCNGC 6 cut(s) 44, 47, 50, 83, 486, 489
Sau3AI GATC 1 cut(s) 352
Sau96I GGNCC 2 cut(s) 7, 526
ScaI AGTACT 1 cut(s) 539
SchI GAGTC 2 cut(s) 101, 475
SetI ASST 7 cut(s) 9, 71, 187, 199, 235, 482, 566
SfcI CTRYAG 1 cut(s) 383
SfoI GGCGCC 1 cut(s) 42
SinI GGWCC 2 cut(s) 7, 526
SmiMI CAYNNNNRTG 1 cut(s) 345
SmlI CTYRAG 1 cut(s) 277
SmoI CTYRAG 1 cut(s) 277
SpeI ACTAGT 1 cut(s) 313
Sse9I AATT 2 cut(s) 96, 225
SsiI CCGC 4 cut(s) 44, 47, 83, 402
SspDI GGCGCC 1 cut(s) 40
SspMI CTAG 1 cut(s) 314
TaaI ACNGT 2 cut(s) 273, 537
TaqI TCGA 1 cut(s) 105
TasI AATT 2 cut(s) 96, 225
TatI WGTACW 1 cut(s) 537
TauI GCSGC 3 cut(s) 46, 49, 85
TfiI GAWTC 1 cut(s) 551
TseFI GTSAC 1 cut(s) 197
TseI GCWGC 3 cut(s) 49, 485, 488
Tsp45I GTSAC 1 cut(s) 197
TspGWI ACGGA 2 cut(s) 41, 106
VpaK11BI GGWCC 2 cut(s) 7, 526
XmiI GTMKAC 1 cut(s) 105
XspI CTAG 1 cut(s) 314
ZrmI AGTACT 1 cut(s) 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.