Rorug02G0493600

Protein TIC 20-IV, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0008813
Physical Location & Seq
Reverse (-)
62262364 .. 62264709
2346 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0493600.1

Sequence Viewer

Length: 1371 bp
ATGACAGAGAGCAATGTGAATGAGCACTACTTGATCGAAATTAAAGAAGAGGAAGAGAGTGTGATAGCAGCAAACAATGTGAAAAACCAAACTTCGAGTGGAAATCAAAATGAGGAGAGTGTCAAGATAGCATCCTCCATTAGCAGAAAGCTACTTAGCAAAGCACCATCACCACCTAGTACTTGCATATACAGAGTCCCTGATGCACTAAAGAAGCTTCACGAAAATGATTTTGTTCCTAGTTTAGTTTCAATTGGACCATTTCACCATGGCACAAAGAATCTGCAAGCCATGGAAGAATTTAAGCTTTGGTACTTGCACCAGCTCCTTGATCGAAAACCCAATCCTGAGACCAAATTGGAGCACATTGTTGAAAAAATCAGAAGCATGGAAGACTATTGTCGTGAATGCTATAACGAAAAACTTGATCATCTGAGCAGTGAAGCATTTGTAGAAATGATGGTGGTTGACGGTTGCTTTATTCTTGAATACTACCGCAAAAGAATAGGGGAAGTGCCTGCAGATGCGGATGATCCACTGTTCAATATTAGTTTAGCAATGTTGCCAACACTTACAAGTGACTTGGTTATGCTAGAAAACCAGCTGCCTTGGAGAGTTCTTGACTGTTTATTCCAACTCACAAAAGTACCTAATGGAATGTACAAGTCACTATCTGAGCTTACTCGCAGTTGTCTCCCAATTTGGGGGACCGTGTCTGACGATCATCTAGAGCAGACATTGGAAAGCAGACATTTACTTGACAATGTCAGGATCCTTTCTGCTGAATTAGACAAGAAAGAGGAGATTATGCCATTTTGGGACTCTATGCCCTCTGCATCGCAACTTCAGCAAATTGGAGTCATATTTCAACCCAAAAGAAATAACATCAACATAAACATAACCTTCCTCGAGGGAGTGATGGAGATTGCACCAATAACCATCCAGGGTGATAATCCTGTCTTTAGAAACCTCATCGCCTTGGAACAGTGTGAATCAGGGCCCCATAAGTATCAAATTAGCACTTATGCCAGGGCATTGGGTGACCTTATTAAATCCAGTAAAGATGTGGAGTTTCTCATGGAGAAAGAGATTATTCATACCAGTTTAAACAAGGAAGACATAGCCGGTTTCTTCAACGACATTTGCAATGGTTTTGCAACTTCCTCCTGCTGCAAGTCTTTTTGGCAAGTTTCCTTGACAGTGAGACAGTATTATAAACAACATTGGCTACAAAGATTGATAGTATACATCAAACATAACTACTTGTACAATCCCTCATCAATATGGTCAGTTTCTAATGCAATTGTCCTTATTATAATTCTTACGGTCCTGCAAACCATATATTCGGTCCTCTCTTACTACAAGTCATAG

Protein Analysis

456

Amino Acids

52.39

Weight (kDa)

5.46

Isoelectric Point (pI)

52.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 63 - 439 1.2e-92 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1215, 1316
AbsI CCTCGAGG 1 cut(s) 908
AccI GTMKAC 1 cut(s) 1245
AciI CCGC 2 cut(s) 496, 527
AclWI GGATC 3 cut(s) 527, 766, 779
AcsI RAATTY 1 cut(s) 299
AcuI CTGAAG 1 cut(s) 830
AfaI GTAC 5 cut(s) 181, 314, 648, 662, 1268
AfiI CCNNNNNNNGG 2 cut(s) 703, 704
AgsI TTSAA 6 cut(s) 252, 374, 488, 544, 869, 1135
AjnI CCWGG 2 cut(s) 942, 1028
AluBI AGCT 6 cut(s) 151, 217, 307, 325, 604, 679
AluI AGCT 6 cut(s) 151, 217, 307, 325, 604, 679
Alw21I GWGCWC 2 cut(s) 27, 366
Alw26I GTCTC 3 cut(s) 344, 698, 1198
AlwI GGATC 3 cut(s) 527, 766, 779
Ama87I CYCGRG 1 cut(s) 908
AoxI GGCC 1 cut(s) 998
ApaI GGGCCC 1 cut(s) 1002
ApeKI GCWGC 3 cut(s) 68, 604, 1170
ApoI RAATTY 1 cut(s) 299
AspS9I GGNCC 6 cut(s) 257, 708, 998, 999, 1327, 1348
AsuHPI GGTGA 4 cut(s) 162, 257, 959, 1052
AvaI CYCGRG 1 cut(s) 908
AvaII GGWCC 4 cut(s) 257, 708, 1327, 1348
BaeGI GKGCMC 1 cut(s) 1002
BaeI ACNNNNGTAYC 2 cut(s) 630, 663
BamHI GGATCC 1 cut(s) 771
BanII GRGCYC 1 cut(s) 1002
BbsI GAAGAC 2 cut(s) 399, 1122
Bbv12I GWGCWC 2 cut(s) 27, 366
BbvI GCAGC 3 cut(s) 80, 591, 1157
BccI CCATC 4 cut(s) 175, 454, 913, 947
BciT130I CCWGG 2 cut(s) 944, 1030
BclI TGATCA 1 cut(s) 427
BcoDI GTCTC 3 cut(s) 344, 698, 1198
BfaI CTAG 4 cut(s) 177, 240, 593, 728
BfmI CTRYAG 1 cut(s) 519
BisI GCNGC 3 cut(s) 69, 605, 1171
BlsI GCNGC 3 cut(s) 70, 606, 1172
BmcAI AGTACT 1 cut(s) 181
Bme1390I CCNGG 2 cut(s) 944, 1030
Bme18I GGWCC 4 cut(s) 257, 708, 1327, 1348
BmeT110I CYCGRG 1 cut(s) 908
BmgT120I GGNCC 6 cut(s) 257, 708, 998, 999, 1327, 1348
BmiI GGNNCC 4 cut(s) 709, 773, 1000, 1001
BmrFI CCNGG 2 cut(s) 944, 1030
BmsI GCATC 4 cut(s) 140, 193, 514, 845
BoxI GACNNNNGTC 1 cut(s) 399
BpiI GAAGAC 2 cut(s) 399, 1122
BsaI GGTCTC 1 cut(s) 344
BsaJI CCNNGG 6 cut(s) 268, 291, 608, 943, 978, 1029
Bsc4I CCNNNNNNNGG 2 cut(s) 703, 704
Bse118I RCCGGY 1 cut(s) 1124
Bse1I ACTGG 2 cut(s) 1056, 1101
Bse3DI GCAATG 3 cut(s) 19, 564, 1153
BseBI CCWGG 2 cut(s) 944, 1030
BseDI CCNNGG 6 cut(s) 268, 291, 608, 943, 978, 1029
BseGI GGATG 3 cut(s) 131, 535, 939
BseLI CCNNNNNNNGG 2 cut(s) 703, 704
BseMI GCAATG 3 cut(s) 19, 564, 1153
BseMII CTCAG 3 cut(s) 339, 425, 666
BseNI ACTGG 2 cut(s) 1056, 1101
BseRI GAGGAG 2 cut(s) 128, 815
BseSI GKGCMC 1 cut(s) 1002
BseXI GCAGC 3 cut(s) 80, 591, 1157
BshFI GGCC 1 cut(s) 1000
BsiHKAI GWGCWC 2 cut(s) 27, 366
BsiHKCI CYCGRG 1 cut(s) 908
BsiSI CCGG 1 cut(s) 1125
BslFI GGGAC 3 cut(s) 182, 721, 833
BslI CCNNNNNNNGG 2 cut(s) 703, 704
BsmAI GTCTC 3 cut(s) 344, 698, 1198
BsmFI GGGAC 3 cut(s) 182, 721, 833
BsmI GAATGC 1 cut(s) 413
BsnI GGCC 1 cut(s) 1000
Bso31I GGTCTC 1 cut(s) 344
BsoBI CYCGRG 1 cut(s) 908
Bsp120I GGGCCC 1 cut(s) 998
Bsp1286I GDGCHC 3 cut(s) 27, 366, 1002
Bsp1407I TGTACA 2 cut(s) 660, 1266
Bsp143I GATC 6 cut(s) 33, 331, 427, 532, 721, 771
Bsp19I CCATGG 2 cut(s) 268, 291
BspACI CCGC 2 cut(s) 496, 527
BspANI GGCC 1 cut(s) 1000
BspCNI CTCAG 3 cut(s) 340, 426, 667
BspLI GGNNCC 4 cut(s) 709, 773, 1000, 1001
BspMAI CTGCAG 1 cut(s) 523
BspPI GGATC 3 cut(s) 527, 766, 779
BspTNI GGTCTC 1 cut(s) 344
BsrDI GCAATG 3 cut(s) 19, 564, 1153
BsrFI RCCGGY 1 cut(s) 1124
BsrGI TGTACA 2 cut(s) 660, 1266
BsrI ACTGG 2 cut(s) 1056, 1101
BssAI RCCGGY 1 cut(s) 1124
BssECI CCNNGG 6 cut(s) 268, 291, 608, 943, 978, 1029
BssMI GATC 6 cut(s) 33, 331, 427, 532, 721, 771
BssNAI GTATAC 1 cut(s) 1246
BssT1I CCWWGG 4 cut(s) 268, 291, 608, 978
Bst1107I GTATAC 1 cut(s) 1246
Bst2UI CCWGG 2 cut(s) 944, 1030
Bst4CI ACNGT 8 cut(s) 473, 540, 626, 712, 987, 1201, 1209, 1327
Bst6I CTCTTC 2 cut(s) 42, 48
BstAUI TGTACA 2 cut(s) 660, 1266
BstC8I GCNNGC 2 cut(s) 288, 519
BstDEI CTNAG 4 cut(s) 155, 348, 434, 675
BstDSI CCRYGG 2 cut(s) 268, 291
BstEII GGTNACC 1 cut(s) 1040
BstF5I GGATG 3 cut(s) 131, 535, 939
BstKTI GATC 6 cut(s) 36, 334, 430, 535, 724, 774
BstMAI GTCTC 3 cut(s) 344, 698, 1198
BstMBI GATC 6 cut(s) 33, 331, 427, 532, 721, 771
BstMWI GCNNNNNNNGC 1 cut(s) 847
BstNI CCWGG 2 cut(s) 944, 1030
BstPAI GACNNNNGTC 1 cut(s) 399
BstPI GGTNACC 1 cut(s) 1040
BstSCI CCNGG 2 cut(s) 942, 1028
BstSFI CTRYAG 1 cut(s) 519
BstSLI GKGCMC 1 cut(s) 1002
BstV1I GCAGC 3 cut(s) 80, 591, 1157
BstV2I GAAGAC 2 cut(s) 399, 1122
BstX2I RGATCY 1 cut(s) 771
BstXI CCANNNNNNTGG 1 cut(s) 1036
BstYI RGATCY 1 cut(s) 771
BstZ17I GTATAC 1 cut(s) 1246
BsuRI GGCC 1 cut(s) 1000
BtgI CCRYGG 2 cut(s) 268, 291
BtgZI GCGATG 2 cut(s) 822, 958
BtsCI GGATG 3 cut(s) 131, 535, 939
BtsI GCAGTG 1 cut(s) 445
BtsIMutI CAGTG 4 cut(s) 445, 536, 992, 1206
Cac8I GCNNGC 2 cut(s) 288, 519
Cfr10I RCCGGY 1 cut(s) 1124
Cfr13I GGNCC 6 cut(s) 257, 708, 998, 999, 1327, 1348
Csp6I GTAC 5 cut(s) 180, 313, 647, 661, 1267
CviAII CATG 4 cut(s) 269, 292, 388, 1078
CviQI GTAC 5 cut(s) 180, 313, 647, 661, 1267
DdeI CTNAG 4 cut(s) 155, 348, 434, 675
DpnI GATC 6 cut(s) 35, 333, 429, 534, 723, 773
DpnII GATC 6 cut(s) 33, 331, 427, 532, 721, 771
DraI TTTAAA 1 cut(s) 1107
Eam1104I CTCTTC 2 cut(s) 42, 48
EarI CTCTTC 2 cut(s) 42, 48
Eco130I CCWWGG 4 cut(s) 268, 291, 608, 978
Eco24I GRGCYC 1 cut(s) 1002
Eco31I GGTCTC 1 cut(s) 344
Eco47I GGWCC 4 cut(s) 257, 708, 1327, 1348
Eco57I CTGAAG 1 cut(s) 830
Eco88I CYCGRG 1 cut(s) 908
Eco91I GGTNACC 1 cut(s) 1040
EcoO109I RGGNCCY 2 cut(s) 998, 999
EcoO65I GGTNACC 1 cut(s) 1040
EcoRII CCWGG 2 cut(s) 942, 1028
EcoT14I CCWWGG 4 cut(s) 268, 291, 608, 978
EcoT38I GRGCYC 1 cut(s) 1002
ErhI CCWWGG 4 cut(s) 268, 291, 608, 978
FaeI CATG 4 cut(s) 272, 295, 391, 1081
FaqI GGGAC 3 cut(s) 182, 721, 833
FatI CATG 4 cut(s) 268, 291, 387, 1077
FbaI TGATCA 1 cut(s) 427
FblI GTMKAC 1 cut(s) 1245
Fnu4HI GCNGC 3 cut(s) 69, 605, 1171
FokI GGATG 3 cut(s) 118, 542, 926
FriOI GRGCYC 1 cut(s) 1002
Fsp4HI GCNGC 3 cut(s) 69, 605, 1171
FspBI CTAG 4 cut(s) 177, 240, 593, 728
GluI GCNGC 3 cut(s) 69, 605, 1171
HaeIII GGCC 1 cut(s) 1000
HapII CCGG 1 cut(s) 1125
Hin1II CATG 4 cut(s) 272, 295, 391, 1081
HincII GTYRAC 1 cut(s) 469
HindII GTYRAC 1 cut(s) 469
HindIII AAGCTT 2 cut(s) 215, 305
HinfI GANTC 5 cut(s) 195, 280, 821, 858, 992
HpaII CCGG 1 cut(s) 1125
HphI GGTGA 4 cut(s) 162, 257, 959, 1052
Hpy166II GTNNAC 2 cut(s) 469, 1246
Hpy188I TCNGA 4 cut(s) 383, 435, 676, 718
Hpy188III TCNNGA 8 cut(s) 124, 221, 347, 404, 485, 620, 728, 769
Hpy8I GTNNAC 2 cut(s) 469, 1246
HpyAV CCTTC 1 cut(s) 913
HpyCH4III ACNGT 8 cut(s) 473, 540, 626, 712, 987, 1201, 1209, 1327
HpyF10VI GCNNNNNNNGC 1 cut(s) 847
HpyF3I CTNAG 4 cut(s) 155, 348, 434, 675
Hsp92II CATG 4 cut(s) 272, 295, 391, 1081
Ksp22I TGATCA 1 cut(s) 427
Kzo9I GATC 6 cut(s) 33, 331, 427, 532, 721, 771
LmnI GCTCC 2 cut(s) 330, 361
Lsp1109I GCAGC 3 cut(s) 80, 591, 1157
LweI GCATC 4 cut(s) 140, 193, 514, 845
MaeI CTAG 4 cut(s) 177, 240, 593, 728
MaeIII GTNAC 3 cut(s) 578, 666, 1040
MalI GATC 6 cut(s) 35, 333, 429, 534, 723, 773
MboI GATC 6 cut(s) 33, 331, 427, 532, 721, 771
MboII GAAGA 6 cut(s) 59, 65, 308, 404, 1123, 1127
MfeI CAATTG 2 cut(s) 252, 1302
MflI RGATCY 1 cut(s) 771
MhlI GDGCHC 3 cut(s) 27, 366, 1002
MlyI GAGTC 3 cut(s) 204, 815, 867
MmeI TCCRAC 1 cut(s) 658
MseI TTAA 4 cut(s) 42, 303, 1050, 1106
MslI CAYNNNNRTG 2 cut(s) 225, 1282
MspA1I CMGCKG 1 cut(s) 604
MspI CCGG 1 cut(s) 1125
MspR9I CCNGG 2 cut(s) 944, 1030
MssI GTTTAAAC 1 cut(s) 1107
MunI CAATTG 2 cut(s) 252, 1302
Mva1269I GAATGC 1 cut(s) 413
MvaI CCWGG 2 cut(s) 944, 1030
MwoI GCNNNNNNNGC 1 cut(s) 847
NcoI CCATGG 2 cut(s) 268, 291
NdeII GATC 6 cut(s) 33, 331, 427, 532, 721, 771
NlaIII CATG 4 cut(s) 272, 295, 391, 1081
NlaIV GGNNCC 4 cut(s) 709, 773, 1000, 1001
NmuCI GTSAC 3 cut(s) 578, 666, 1040
PaeR7I CTCGAG 1 cut(s) 908
PctI GAATGC 1 cut(s) 413
PfeI GAWTC 2 cut(s) 280, 992
PflFI GACNNNGTC 2 cut(s) 712, 764
PkrI GCNGC 3 cut(s) 70, 606, 1172
PleI GAGTC 3 cut(s) 203, 815, 866
PmeI GTTTAAAC 1 cut(s) 1107
PpsI GAGTC 3 cut(s) 203, 815, 866
PshAI GACNNNNGTC 1 cut(s) 399
PsiI TTATAA 2 cut(s) 1215, 1316
Psp6I CCWGG 2 cut(s) 942, 1028
PspEI GGTNACC 1 cut(s) 1040
PspGI CCWGG 2 cut(s) 942, 1028
PspN4I GGNNCC 4 cut(s) 709, 773, 1000, 1001
PspOMI GGGCCC 1 cut(s) 998
PspPI GGNCC 6 cut(s) 257, 708, 998, 999, 1327, 1348
PspXI VCTCGAGB 1 cut(s) 908
PstI CTGCAG 1 cut(s) 523
PsuI RGATCY 1 cut(s) 771
PsyI GACNNNGTC 2 cut(s) 712, 764
PvuII CAGCTG 1 cut(s) 604
RsaI GTAC 5 cut(s) 181, 314, 648, 662, 1268
RsaNI GTAC 5 cut(s) 180, 313, 647, 661, 1267
RseI CAYNNNNRTG 2 cut(s) 225, 1282
SaqAI TTAA 4 cut(s) 42, 303, 1050, 1106
SatI GCNGC 3 cut(s) 69, 605, 1171
Sau3AI GATC 6 cut(s) 33, 331, 427, 532, 721, 771
Sau96I GGNCC 6 cut(s) 257, 708, 998, 999, 1327, 1348
ScaI AGTACT 1 cut(s) 181
SchI GAGTC 3 cut(s) 204, 815, 867
ScrFI CCNGG 2 cut(s) 944, 1030
SduI GDGCHC 3 cut(s) 27, 366, 1002
SfaNI GCATC 4 cut(s) 140, 193, 514, 845
SfcI CTRYAG 1 cut(s) 519
Sfr274I CTCGAG 1 cut(s) 908
SinI GGWCC 4 cut(s) 257, 708, 1327, 1348
SlaI CTCGAG 1 cut(s) 908
SmiMI CAYNNNNRTG 2 cut(s) 225, 1282
SmlI CTYRAG 1 cut(s) 908
SmoI CTYRAG 1 cut(s) 908
SsiI CCGC 2 cut(s) 496, 527
SspI AATATT 1 cut(s) 547
SspMI CTAG 4 cut(s) 177, 240, 593, 728
StyD4I CCNGG 2 cut(s) 942, 1028
StyI CCWWGG 4 cut(s) 268, 291, 608, 978
TaaI ACNGT 8 cut(s) 473, 540, 626, 712, 987, 1201, 1209, 1327
TaqI TCGA 4 cut(s) 36, 95, 334, 909
TaqII GACCGA 1 cut(s) 1336
TatI WGTACW 3 cut(s) 179, 660, 1266
TfiI GAWTC 2 cut(s) 280, 992
Tru1I TTAA 4 cut(s) 42, 303, 1050, 1106
Tru9I TTAA 4 cut(s) 42, 303, 1050, 1106
TscAI CASTG 4 cut(s) 445, 543, 992, 1206
TseFI GTSAC 3 cut(s) 578, 666, 1040
TseI GCWGC 3 cut(s) 68, 604, 1170
Tsp45I GTSAC 3 cut(s) 578, 666, 1040
TspDTI ATGAA 1 cut(s) 1085
TspRI CASTG 4 cut(s) 445, 543, 992, 1206
Tth111I GACNNNGTC 2 cut(s) 712, 764
VpaK11BI GGWCC 4 cut(s) 257, 708, 1327, 1348
XapI RAATTY 1 cut(s) 299
XbaI TCTAGA 1 cut(s) 727
XcmI CCANNNNNNNNNTGG 2 cut(s) 95, 1063
XhoI CTCGAG 1 cut(s) 908
XmiI GTMKAC 1 cut(s) 1245
XspI CTAG 4 cut(s) 177, 240, 593, 728
ZrmI AGTACT 1 cut(s) 181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.