Rh2CG543000

Protein TIC 20-IV, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0008813
Physical Location & Seq
Forward (+)
72162761 .. 72166613
3853 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG543000.1

Sequence Viewer

Length: 915 bp
ATGAGCAGTATTCTATCACTGAATCACAAGGCGGTTATTGATATGATCGTATATGTATCTTATGCTCCTACAGGCCTAGTTCATGATAAACTTCTTATGTGCTGTTGGTTTTTTACTAGGTACAGGGATGTGAAGCTTAGACGACTCACCAAGCCAAACAGGGTTGCATGCAACTTCACTCCAAAGTCAATGCCTATATTTTCAACCAGCAGGCCACCTAATCAACTTCTGCCTGTACTGCCCAGAGTTTCAGGATTGCCAAGCTTGGACCTTTCTGCTGCATCATCCACAGTGCTAAGGGGGAATGAAGCTGGCCGGTCACAGAAAATATCCATCCTACTCAGACAACGCAAATCTTCAACATTGGTACAAGCAAAGAAGGATAATAGTTCAATCACTATACCTTTCCCGAAAATGACTGAGAAGCCAGAGTGGTGGTGGAGAACTTTAGCATGTCTCCCTTATTTAGCATCTTTGCAGATGTCTGAGGTTGGCTTTTATATTCAACCCTTTGCAGAACACTATCAACTTTTTGGAGATTTGATTTATTTTGTCCCCGGAGCAATAAAGAGGTTACCACAGTGGTTCACAATGACATATGTCATGGTGGCATATTTTGGAGTGGTGAAGAAGAAACAGTGGCCTCACTTCTTTAGGTACCATGTTATTATGGCCATGTTATTAGAAACGATGCTGCAAGTGGTAGCACAGTCGAGCAATTTCTTTCCGCTTATACACTTCAATGGTACATTTGGCATAGAGTATTGGGCGGCTGTGGCATTCCTGTACATTTTGATCTTGTTGGAGTGCATAAGGTGTGCTCTTGCTGGCAAGTATGTTAAGCTCCCGTTTTTCAGTACTCCTGCTCTAGCTCATACTCTTTTCACCGTAGAAGGCAGGTACAGACCTTTTTAA

Protein Analysis

304

Amino Acids

34.99

Weight (kDa)

9.84

Isoelectric Point (pI)

49.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIC20 PF16166 115 - 288 1.8e-68 Chloroplast import apparatus Tic20-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 888
Acc65I GGTACC 1 cut(s) 657
AccB1I GGYRCC 1 cut(s) 657
AciI CCGC 3 cut(s) 32, 728, 770
AcoI YGGCCR 2 cut(s) 313, 672
AfaI GTAC 8 cut(s) 122, 237, 369, 659, 748, 788, 859, 902
AgsI TTSAA 5 cut(s) 204, 360, 393, 506, 742
AluBI AGCT 5 cut(s) 136, 264, 311, 844, 872
AluI AGCT 5 cut(s) 136, 264, 311, 844, 872
Alw21I GWGCWC 1 cut(s) 823
Alw26I GTCTC 1 cut(s) 461
AoxI GGCC 5 cut(s) 73, 212, 313, 641, 672
ApeKI GCWGC 2 cut(s) 278, 694
Asp718I GGTACC 1 cut(s) 657
AspS9I GGNCC 1 cut(s) 268
AsuC2I CCSGG 1 cut(s) 558
AsuHPI GGTGA 3 cut(s) 139, 637, 877
AvaII GGWCC 1 cut(s) 268
BalI TGGCCA 1 cut(s) 674
BanI GGYRCC 1 cut(s) 657
Bbv12I GWGCWC 1 cut(s) 823
BbvI GCAGC 2 cut(s) 265, 681
BccI CCATC 1 cut(s) 341
BcnI CCSGG 1 cut(s) 558
BcoDI GTCTC 1 cut(s) 461
BfaI CTAG 3 cut(s) 77, 117, 869
BfmI CTRYAG 1 cut(s) 69
BfuAI ACCTGC 1 cut(s) 888
BisI GCNGC 3 cut(s) 279, 695, 771
BlsI GCNGC 3 cut(s) 280, 696, 772
BmcAI AGTACT 1 cut(s) 859
Bme1390I CCNGG 1 cut(s) 558
Bme18I GGWCC 1 cut(s) 268
BmgT120I GGNCC 1 cut(s) 268
BmiI GGNNCC 1 cut(s) 659
BmrFI CCNGG 1 cut(s) 558
BmsI GCATC 3 cut(s) 290, 479, 681
BoxI GACNNNNGTC 1 cut(s) 599
Bpu10I CCTNAGC 1 cut(s) 296
BpuMI CCSGG 1 cut(s) 558
BsaJI CCNNGG 1 cut(s) 556
Bse118I RCCGGY 1 cut(s) 315
BseDI CCNNGG 1 cut(s) 556
BseGI GGATG 3 cut(s) 133, 284, 333
BseMII CTCAG 3 cut(s) 355, 411, 477
BseXI GCAGC 2 cut(s) 265, 681
BshFI GGCC 5 cut(s) 75, 214, 315, 643, 674
BshNI GGYRCC 1 cut(s) 657
BsiHKAI GWGCWC 1 cut(s) 823
BsiSI CCGG 2 cut(s) 316, 558
BslFI GGGAC 1 cut(s) 539
BsmAI GTCTC 1 cut(s) 461
BsmFI GGGAC 1 cut(s) 539
BsmI GAATGC 1 cut(s) 779
BsnI GGCC 5 cut(s) 75, 214, 315, 643, 674
Bsp1286I GDGCHC 1 cut(s) 823
Bsp1407I TGTACA 1 cut(s) 786
Bsp143I GATC 2 cut(s) 45, 795
BspACI CCGC 3 cut(s) 32, 728, 770
BspANI GGCC 5 cut(s) 75, 214, 315, 643, 674
BspCNI CTCAG 3 cut(s) 354, 412, 478
BspHI TCATGA 1 cut(s) 82
BspLI GGNNCC 1 cut(s) 659
BspMI ACCTGC 1 cut(s) 888
BspT107I GGYRCC 1 cut(s) 657
BsrFI RCCGGY 1 cut(s) 315
BsrGI TGTACA 1 cut(s) 786
BssAI RCCGGY 1 cut(s) 315
BssECI CCNNGG 1 cut(s) 556
BssMI GATC 2 cut(s) 45, 795
Bst4CI ACNGT 5 cut(s) 292, 582, 639, 711, 889
BstAUI TGTACA 1 cut(s) 786
BstC8I GCNNGC 4 cut(s) 169, 212, 313, 829
BstDEI CTNAG 5 cut(s) 137, 296, 341, 420, 486
BstEII GGTNACC 1 cut(s) 573
BstF5I GGATG 3 cut(s) 133, 284, 333
BstKTI GATC 2 cut(s) 48, 798
BstMAI GTCTC 1 cut(s) 461
BstMBI GATC 2 cut(s) 45, 795
BstMWI GCNNNNNNNGC 2 cut(s) 238, 776
BstNSI RCATGY 2 cut(s) 171, 456
BstPAI GACNNNNGTC 1 cut(s) 599
BstPI GGTNACC 1 cut(s) 573
BstSCI CCNGG 1 cut(s) 556
BstSFI CTRYAG 1 cut(s) 69
BstV1I GCAGC 2 cut(s) 265, 681
BstXI CCANNNNNNTGG 1 cut(s) 435
BsuRI GGCC 5 cut(s) 75, 214, 315, 643, 674
BtsCI GGATG 3 cut(s) 133, 284, 333
BtsIMutI CAGTG 4 cut(s) 17, 297, 587, 644
BveI ACCTGC 1 cut(s) 888
Cac8I GCNNGC 4 cut(s) 169, 212, 313, 829
CciI TCATGA 1 cut(s) 82
Cfr10I RCCGGY 1 cut(s) 315
Cfr13I GGNCC 1 cut(s) 268
Csp6I GTAC 8 cut(s) 121, 236, 368, 658, 747, 787, 858, 901
CviAII CATG 6 cut(s) 83, 168, 453, 604, 662, 676
CviQI GTAC 8 cut(s) 121, 236, 368, 658, 747, 787, 858, 901
DdeI CTNAG 5 cut(s) 137, 296, 341, 420, 486
DpnI GATC 2 cut(s) 47, 797
DpnII GATC 2 cut(s) 45, 795
EaeI YGGCCR 2 cut(s) 313, 672
Eco147I AGGCCT 1 cut(s) 75
Eco47I GGWCC 1 cut(s) 268
Eco91I GGTNACC 1 cut(s) 573
EcoO65I GGTNACC 1 cut(s) 573
FaeI CATG 6 cut(s) 86, 171, 456, 607, 665, 679
FaqI GGGAC 1 cut(s) 539
FatI CATG 6 cut(s) 82, 167, 452, 603, 661, 675
FauNDI CATATG 1 cut(s) 598
Fnu4HI GCNGC 3 cut(s) 279, 695, 771
FokI GGATG 3 cut(s) 140, 271, 320
Fsp4HI GCNGC 3 cut(s) 279, 695, 771
FspBI CTAG 3 cut(s) 77, 117, 869
GluI GCNGC 3 cut(s) 279, 695, 771
HaeIII GGCC 5 cut(s) 75, 214, 315, 643, 674
HapII CCGG 2 cut(s) 316, 558
Hin1II CATG 6 cut(s) 86, 171, 456, 607, 665, 679
HindIII AAGCTT 2 cut(s) 134, 262
HinfI GANTC 2 cut(s) 22, 144
HpaII CCGG 2 cut(s) 316, 558
HphI GGTGA 3 cut(s) 139, 637, 877
Hpy166II GTNNAC 1 cut(s) 588
Hpy188I TCNGA 2 cut(s) 344, 487
Hpy188III TCNNGA 3 cut(s) 83, 252, 409
Hpy8I GTNNAC 1 cut(s) 588
HpyAV CCTTC 2 cut(s) 373, 887
HpyCH4III ACNGT 5 cut(s) 292, 582, 639, 711, 889
HpyCH4V TGCA 7 cut(s) 167, 171, 281, 478, 515, 697, 810
HpyF10VI GCNNNNNNNGC 2 cut(s) 238, 776
HpyF3I CTNAG 5 cut(s) 137, 296, 341, 420, 486
Hsp92II CATG 6 cut(s) 86, 171, 456, 607, 665, 679
KpnI GGTACC 1 cut(s) 661
Kzo9I GATC 2 cut(s) 45, 795
LmnI GCTCC 3 cut(s) 70, 560, 849
Lsp1109I GCAGC 2 cut(s) 265, 681
LweI GCATC 3 cut(s) 290, 479, 681
MaeI CTAG 3 cut(s) 77, 117, 869
MaeIII GTNAC 2 cut(s) 318, 573
MalI GATC 2 cut(s) 47, 797
MboI GATC 2 cut(s) 45, 795
MboII GAAGA 3 cut(s) 348, 640, 643
MhlI GDGCHC 1 cut(s) 823
MlsI TGGCCA 1 cut(s) 674
MluCI AATT 1 cut(s) 718
MluNI TGGCCA 1 cut(s) 674
MlyI GAGTC 1 cut(s) 138
MmeI TCCRAC 1 cut(s) 783
MnlI CCTC 3 cut(s) 481, 564, 654
Mox20I TGGCCA 1 cut(s) 674
MscI TGGCCA 1 cut(s) 674
MseI TTAA 2 cut(s) 840, 913
MslI CAYNNNNRTG 1 cut(s) 741
Msp20I TGGCCA 1 cut(s) 674
MspI CCGG 2 cut(s) 316, 558
MspR9I CCNGG 1 cut(s) 558
Mva1269I GAATGC 1 cut(s) 779
MwoI GCNNNNNNNGC 2 cut(s) 238, 776
NciI CCSGG 1 cut(s) 558
NdeI CATATG 1 cut(s) 598
NdeII GATC 2 cut(s) 45, 795
NlaIII CATG 6 cut(s) 86, 171, 456, 607, 665, 679
NlaIV GGNNCC 1 cut(s) 659
NmuCI GTSAC 1 cut(s) 318
NspI RCATGY 2 cut(s) 171, 456
PaeI GCATGC 1 cut(s) 171
PagI TCATGA 1 cut(s) 82
PceI AGGCCT 1 cut(s) 75
PctI GAATGC 1 cut(s) 779
PfeI GAWTC 1 cut(s) 22
PkrI GCNGC 3 cut(s) 280, 696, 772
PleI GAGTC 1 cut(s) 138
PpsI GAGTC 1 cut(s) 138
PshAI GACNNNNGTC 1 cut(s) 599
PspEI GGTNACC 1 cut(s) 573
PspN4I GGNNCC 1 cut(s) 659
PspPI GGNCC 1 cut(s) 268
RsaI GTAC 8 cut(s) 122, 237, 369, 659, 748, 788, 859, 902
RsaNI GTAC 8 cut(s) 121, 236, 368, 658, 747, 787, 858, 901
RseI CAYNNNNRTG 1 cut(s) 741
SaqAI TTAA 2 cut(s) 840, 913
SatI GCNGC 3 cut(s) 279, 695, 771
Sau3AI GATC 2 cut(s) 45, 795
Sau96I GGNCC 1 cut(s) 268
ScaI AGTACT 1 cut(s) 859
SchI GAGTC 1 cut(s) 138
ScrFI CCNGG 1 cut(s) 558
SduI GDGCHC 1 cut(s) 823
SfaNI GCATC 3 cut(s) 290, 479, 681
SfcI CTRYAG 1 cut(s) 69
SinI GGWCC 1 cut(s) 268
SmiMI CAYNNNNRTG 1 cut(s) 741
SphI GCATGC 1 cut(s) 171
Sse9I AATT 1 cut(s) 718
SseBI AGGCCT 1 cut(s) 75
SsiI CCGC 3 cut(s) 32, 728, 770
SspMI CTAG 3 cut(s) 77, 117, 869
StuI AGGCCT 1 cut(s) 75
StyD4I CCNGG 1 cut(s) 556
TaaI ACNGT 5 cut(s) 292, 582, 639, 711, 889
TaqI TCGA 1 cut(s) 713
TasI AATT 1 cut(s) 718
TatI WGTACW 3 cut(s) 235, 786, 857
TauI GCSGC 1 cut(s) 773
TfiI GAWTC 1 cut(s) 22
Tru1I TTAA 2 cut(s) 840, 913
Tru9I TTAA 2 cut(s) 840, 913
TscAI CASTG 4 cut(s) 24, 297, 587, 644
TseFI GTSAC 1 cut(s) 318
TseI GCWGC 2 cut(s) 278, 694
Tsp45I GTSAC 1 cut(s) 318
TspDTI ATGAA 2 cut(s) 71, 321
TspRI CASTG 4 cut(s) 24, 297, 587, 644
VpaK11BI GGWCC 1 cut(s) 268
XceI RCATGY 2 cut(s) 171, 456
XcmI CCANNNNNNNNNTGG 1 cut(s) 435
XspI CTAG 3 cut(s) 77, 117, 869
ZrmI AGTACT 1 cut(s) 859
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.