Rh2DG582700

Protein TIC 20-IV, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0008813
Physical Location & Seq
Forward (+)
81343109 .. 81345655
2547 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG582700.1

Sequence Viewer

Length: 855 bp
ATGGCGACCGCCTTGAGCTGCGGCCAGACCATCTTCCTCGTTCCTCCTTCTCGCATCACCTCTCGGTACAGGGATGTGAAACTTAGACGACTCACCAAGCCAAACAGGGTTGCATGCAACTTCACTCCAAAGTCACTGCCTATATTTTCAACCAGCAGACCACCTAATCAACTTCTGCCTGTACTGCCCAGAGGATTGCCAAGCTTGGACCTTTCTGCTGCATCATCCACAGTGCTAAGGGGGAATGAAGCTGGCCGGTCACAGAAAACATCCATCCTACTCAGACAACGCAAATCTTCAACATTGGTACAAGCAAAGAAGGATAATAGTTCAATCACTATACCTTTCCCGAAAATGACTGAGAAGCCAGAGTGGTGGTGGAGAACTTTAGCATGTCTCCCTTATTTAGCATCTTTGCAGATGTCCGAGGTTGGCTTTTATATTCAACCCTTTGCAGAACACTATCAACTTTTTGGAGATTTGATTTATTTTGTCCCCGGAGCAATAAAGAGGTTACCACAGTGGTTCACAATGACATATGTCATGGTGGCATATTTTGGAGTGGTGAAGAAGAAACAGTGGCCTCACTTCTTTAGGTACCATGTTATTATGGCCATGTTATTAGAAACGATGCTGCAAGTGGTAGCGCAGTCGAGCAATTTCTTTCCGCTTATACACTTCAATGGTACATTTGGCATAGAGTATTGGGCGGCTGTGGCATTCCTGTACATTTTGATCTTGTTGGAGTGCATAAGGTGTGCTCTTGCTGGCAAGTATGTTAAGCTCCCGTTTTTCAGTACTCCTGCTCTAGCTCATACTCTTTTCACCGTAGAAGGCAGGTACAGACCTTTTTAA

Protein Analysis

284

Amino Acids

32.52

Weight (kDa)

10.05

Isoelectric Point (pI)

52.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIC20 PF16166 95 - 268 1.5e-68 Chloroplast import apparatus Tic20-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 828
Acc65I GGTACC 1 cut(s) 597
AccB1I GGYRCC 1 cut(s) 597
AciI CCGC 4 cut(s) 9, 21, 668, 710
AcoI YGGCCR 3 cut(s) 22, 253, 612
AfaI GTAC 8 cut(s) 68, 183, 309, 599, 688, 728, 799, 842
AgsI TTSAA 5 cut(s) 150, 300, 333, 446, 682
AluBI AGCT 5 cut(s) 18, 204, 251, 784, 812
AluI AGCT 5 cut(s) 18, 204, 251, 784, 812
Alw21I GWGCWC 1 cut(s) 763
Alw26I GTCTC 1 cut(s) 401
AoxI GGCC 4 cut(s) 22, 253, 581, 612
ApeKI GCWGC 3 cut(s) 18, 218, 634
Asp718I GGTACC 1 cut(s) 597
AspLEI GCGC 1 cut(s) 649
AspS9I GGNCC 1 cut(s) 208
AsuC2I CCSGG 1 cut(s) 498
AsuHPI GGTGA 4 cut(s) 49, 85, 577, 817
AvaII GGWCC 1 cut(s) 208
BalI TGGCCA 1 cut(s) 614
BanI GGYRCC 1 cut(s) 597
Bbv12I GWGCWC 1 cut(s) 763
BbvI GCAGC 3 cut(s) 5, 205, 621
BccI CCATC 2 cut(s) 38, 281
BcnI CCSGG 1 cut(s) 498
BcoDI GTCTC 1 cut(s) 401
BfaI CTAG 1 cut(s) 809
BfuAI ACCTGC 1 cut(s) 828
BisI GCNGC 5 cut(s) 19, 22, 219, 635, 711
BlsI GCNGC 5 cut(s) 20, 23, 220, 636, 712
BmcAI AGTACT 1 cut(s) 799
Bme1390I CCNGG 1 cut(s) 498
Bme18I GGWCC 1 cut(s) 208
BmgT120I GGNCC 1 cut(s) 208
BmiI GGNNCC 1 cut(s) 599
BmrFI CCNGG 1 cut(s) 498
BmsI GCATC 4 cut(s) 63, 230, 419, 621
BoxI GACNNNNGTC 1 cut(s) 539
Bpu10I CCTNAGC 1 cut(s) 236
BpuEI CTTGAG 1 cut(s) 34
BpuMI CCSGG 1 cut(s) 498
BsaJI CCNNGG 2 cut(s) 426, 496
Bse118I RCCGGY 1 cut(s) 255
BseDI CCNNGG 2 cut(s) 426, 496
BseGI GGATG 4 cut(s) 79, 224, 269, 273
BseMII CTCAG 2 cut(s) 295, 351
BseXI GCAGC 3 cut(s) 5, 205, 621
Bsh1285I CGRYCG 1 cut(s) 9
BshFI GGCC 4 cut(s) 24, 255, 583, 614
BshNI GGYRCC 1 cut(s) 597
BsiEI CGRYCG 1 cut(s) 9
BsiHKAI GWGCWC 1 cut(s) 763
BsiSI CCGG 2 cut(s) 256, 498
BslFI GGGAC 1 cut(s) 479
BsmAI GTCTC 1 cut(s) 401
BsmFI GGGAC 1 cut(s) 479
BsmI GAATGC 1 cut(s) 719
BsnI GGCC 4 cut(s) 24, 255, 583, 614
Bsp1286I GDGCHC 1 cut(s) 763
Bsp1407I TGTACA 1 cut(s) 726
Bsp143I GATC 1 cut(s) 735
BspACI CCGC 4 cut(s) 9, 21, 668, 710
BspANI GGCC 4 cut(s) 24, 255, 583, 614
BspCNI CTCAG 2 cut(s) 294, 352
BspLI GGNNCC 1 cut(s) 599
BspMI ACCTGC 1 cut(s) 828
BspT107I GGYRCC 1 cut(s) 597
BsrFI RCCGGY 1 cut(s) 255
BsrGI TGTACA 1 cut(s) 726
BssAI RCCGGY 1 cut(s) 255
BssECI CCNNGG 2 cut(s) 426, 496
BssMI GATC 1 cut(s) 735
Bst4CI ACNGT 4 cut(s) 232, 522, 579, 829
BstAUI TGTACA 1 cut(s) 726
BstC8I GCNNGC 3 cut(s) 115, 253, 769
BstDEI CTNAG 4 cut(s) 83, 236, 281, 360
BstEII GGTNACC 1 cut(s) 513
BstF5I GGATG 4 cut(s) 79, 224, 269, 273
BstHHI GCGC 1 cut(s) 649
BstKTI GATC 1 cut(s) 738
BstMAI GTCTC 1 cut(s) 401
BstMBI GATC 1 cut(s) 735
BstMCI CGRYCG 1 cut(s) 9
BstMWI GCNNNNNNNGC 2 cut(s) 184, 716
BstNSI RCATGY 2 cut(s) 117, 396
BstPAI GACNNNNGTC 1 cut(s) 539
BstPI GGTNACC 1 cut(s) 513
BstSCI CCNGG 1 cut(s) 496
BstV1I GCAGC 3 cut(s) 5, 205, 621
BstXI CCANNNNNNTGG 1 cut(s) 375
BsuRI GGCC 4 cut(s) 24, 255, 583, 614
BtsCI GGATG 4 cut(s) 79, 224, 269, 273
BtsI GCAGTG 1 cut(s) 134
BtsIMutI CAGTG 4 cut(s) 134, 237, 527, 584
BveI ACCTGC 1 cut(s) 828
Cac8I GCNNGC 3 cut(s) 115, 253, 769
CfoI GCGC 1 cut(s) 649
Cfr10I RCCGGY 1 cut(s) 255
Cfr13I GGNCC 1 cut(s) 208
Csp6I GTAC 8 cut(s) 67, 182, 308, 598, 687, 727, 798, 841
CviAII CATG 5 cut(s) 114, 393, 544, 602, 616
CviQI GTAC 8 cut(s) 67, 182, 308, 598, 687, 727, 798, 841
DdeI CTNAG 4 cut(s) 83, 236, 281, 360
DpnI GATC 1 cut(s) 737
DpnII GATC 1 cut(s) 735
EaeI YGGCCR 3 cut(s) 22, 253, 612
Eco47I GGWCC 1 cut(s) 208
Eco91I GGTNACC 1 cut(s) 513
EcoO65I GGTNACC 1 cut(s) 513
FaeI CATG 5 cut(s) 117, 396, 547, 605, 619
FaqI GGGAC 1 cut(s) 479
FatI CATG 5 cut(s) 113, 392, 543, 601, 615
FauNDI CATATG 1 cut(s) 538
Fnu4HI GCNGC 5 cut(s) 19, 22, 219, 635, 711
FokI GGATG 4 cut(s) 86, 211, 256, 260
Fsp4HI GCNGC 5 cut(s) 19, 22, 219, 635, 711
FspBI CTAG 1 cut(s) 809
GlaI GCGC 1 cut(s) 648
GluI GCNGC 5 cut(s) 19, 22, 219, 635, 711
HaeIII GGCC 4 cut(s) 24, 255, 583, 614
HapII CCGG 2 cut(s) 256, 498
HhaI GCGC 1 cut(s) 649
Hin1II CATG 5 cut(s) 117, 396, 547, 605, 619
Hin6I GCGC 1 cut(s) 647
HinP1I GCGC 1 cut(s) 647
HindIII AAGCTT 1 cut(s) 202
HinfI GANTC 1 cut(s) 90
HpaII CCGG 2 cut(s) 256, 498
HphI GGTGA 4 cut(s) 49, 85, 577, 817
Hpy166II GTNNAC 1 cut(s) 528
Hpy188I TCNGA 2 cut(s) 284, 427
Hpy188III TCNNGA 1 cut(s) 349
Hpy8I GTNNAC 1 cut(s) 528
HpyAV CCTTC 3 cut(s) 57, 313, 827
HpyCH4III ACNGT 4 cut(s) 232, 522, 579, 829
HpyCH4V TGCA 7 cut(s) 113, 117, 221, 418, 455, 637, 750
HpyF10VI GCNNNNNNNGC 2 cut(s) 184, 716
HpyF3I CTNAG 4 cut(s) 83, 236, 281, 360
Hsp92II CATG 5 cut(s) 117, 396, 547, 605, 619
HspAI GCGC 1 cut(s) 647
KpnI GGTACC 1 cut(s) 601
Kzo9I GATC 1 cut(s) 735
LmnI GCTCC 2 cut(s) 500, 789
Lsp1109I GCAGC 3 cut(s) 5, 205, 621
LweI GCATC 4 cut(s) 63, 230, 419, 621
MaeI CTAG 1 cut(s) 809
MaeIII GTNAC 3 cut(s) 132, 258, 513
MalI GATC 1 cut(s) 737
MboI GATC 1 cut(s) 735
MboII GAAGA 4 cut(s) 25, 288, 580, 583
MhlI GDGCHC 1 cut(s) 763
MlsI TGGCCA 1 cut(s) 614
MluCI AATT 1 cut(s) 658
MluNI TGGCCA 1 cut(s) 614
MlyI GAGTC 1 cut(s) 84
MmeI TCCRAC 1 cut(s) 723
MnlI CCTC 7 cut(s) 47, 54, 70, 185, 421, 504, 594
Mox20I TGGCCA 1 cut(s) 614
MscI TGGCCA 1 cut(s) 614
MseI TTAA 2 cut(s) 780, 853
MslI CAYNNNNRTG 1 cut(s) 681
Msp20I TGGCCA 1 cut(s) 614
MspI CCGG 2 cut(s) 256, 498
MspR9I CCNGG 1 cut(s) 498
Mva1269I GAATGC 1 cut(s) 719
MwoI GCNNNNNNNGC 2 cut(s) 184, 716
NciI CCSGG 1 cut(s) 498
NdeI CATATG 1 cut(s) 538
NdeII GATC 1 cut(s) 735
NlaIII CATG 5 cut(s) 117, 396, 547, 605, 619
NlaIV GGNNCC 1 cut(s) 599
NmuCI GTSAC 2 cut(s) 132, 258
NspI RCATGY 2 cut(s) 117, 396
PaeI GCATGC 1 cut(s) 117
PctI GAATGC 1 cut(s) 719
PkrI GCNGC 5 cut(s) 20, 23, 220, 636, 712
PleI GAGTC 1 cut(s) 84
PpsI GAGTC 1 cut(s) 84
PshAI GACNNNNGTC 1 cut(s) 539
PspEI GGTNACC 1 cut(s) 513
PspN4I GGNNCC 1 cut(s) 599
PspPI GGNCC 1 cut(s) 208
RsaI GTAC 8 cut(s) 68, 183, 309, 599, 688, 728, 799, 842
RsaNI GTAC 8 cut(s) 67, 182, 308, 598, 687, 727, 798, 841
RseI CAYNNNNRTG 1 cut(s) 681
SaqAI TTAA 2 cut(s) 780, 853
SatI GCNGC 5 cut(s) 19, 22, 219, 635, 711
Sau3AI GATC 1 cut(s) 735
Sau96I GGNCC 1 cut(s) 208
ScaI AGTACT 1 cut(s) 799
SchI GAGTC 1 cut(s) 84
ScrFI CCNGG 1 cut(s) 498
SduI GDGCHC 1 cut(s) 763
SfaNI GCATC 4 cut(s) 63, 230, 419, 621
SinI GGWCC 1 cut(s) 208
SmiMI CAYNNNNRTG 1 cut(s) 681
SmlI CTYRAG 1 cut(s) 13
SmoI CTYRAG 1 cut(s) 13
SphI GCATGC 1 cut(s) 117
Sse9I AATT 1 cut(s) 658
SsiI CCGC 4 cut(s) 9, 21, 668, 710
SspMI CTAG 1 cut(s) 809
StyD4I CCNGG 1 cut(s) 496
TaaI ACNGT 4 cut(s) 232, 522, 579, 829
TaqI TCGA 1 cut(s) 653
TasI AATT 1 cut(s) 658
TatI WGTACW 3 cut(s) 181, 726, 797
TauI GCSGC 2 cut(s) 24, 713
Tru1I TTAA 2 cut(s) 780, 853
Tru9I TTAA 2 cut(s) 780, 853
TscAI CASTG 4 cut(s) 141, 237, 527, 584
TseFI GTSAC 2 cut(s) 132, 258
TseI GCWGC 3 cut(s) 18, 218, 634
Tsp45I GTSAC 2 cut(s) 132, 258
TspDTI ATGAA 1 cut(s) 261
TspRI CASTG 4 cut(s) 141, 237, 527, 584
VpaK11BI GGWCC 1 cut(s) 208
XceI RCATGY 2 cut(s) 117, 396
XcmI CCANNNNNNNNNTGG 1 cut(s) 375
XspI CTAG 1 cut(s) 809
ZrmI AGTACT 1 cut(s) 799
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.