Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0012686

Physical Location & Seq

Genomic Coordinates Reverse (-)

72256458 .. 72259058

Gene Structure 2601 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0604700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2601 bp

ATGGCCGATCACCACCATCACCACCACCGCCCCCACCGCCTCTCCCTCCCTCAACGCCCCACCACGCCCACCACCTTCGCCTCCACCACCCCCACCGCCTCCCGCCAATACCCACTTTACCCCttcactccctccgccGCCACTCCCTCCAAACACCGCCTCTCTTCTTCTCTCAACCCCAAACCCCTCTCCGCCGCCGCCGCCGCCGCTGGAAGCAAATCCTCTCTCTCATTCCTCTTCCTCCTCCTCCTCTCCCTCCGATCACTCTACTCCCTCCTCCCCTTCCTCCGCTCCTCCCCTTCCTTCTCTCTCTTCCCCTTCTCCTTCCTCGTCTCTCTCCTCTCCTTCCTCCTAACCCTAGCTTTCTCTCTCTTCACCTCCTCCGCCTCCGCCTCCGCCGCCGCCTCCAGAGACCCGTTCCACCAGAAGCCCAACCAGCCCATCTTCTCCCTCACTCTCATCACTCAATCCCAGCACAGACTCCTCGTCGCGAAATCGATTCTTCTCGCCGGAGTCTTCCTCCTCCGCTTCCAAGCTCTCCGGTACTGCGGCACCGCCGCCATGATCCTAGCCGAGATGTCTGGAAATGTGGCGGCAAGGTTCTTGGCGGAGGGGAGGAAACAGAGTGTGGTGGGCGGCAGTGGTGATCGGAATCGGAGCTCCAAGGTTCGTGGGTTTCTTGCTCTGTTCACTGGGTTGTTCTTGTTATCTATGAGCTGGGATCGGATTGAATGCTTTCCGTTTTCGGCTAAGTTTGTGCAAAAAATGGGAGTGTCTGTGTTGCCTAGAGTGGATTGTGTGAGAATTTGGCCAATGCTGCTTCCGTTTCTGTCTGGATTTTTGGGGTGTTATGAGAGGGTTTCGATGAATTGGGGGACTATTAGGCAGTTGGGGAGGAAGAGGGTTCGGGTGATTTCGTTGTTTTTTACTACTGTTGTGCTGTTCATTCCTGCTGTTGTTAGTGTTTTTATGTTTGAAGCTGAGGGAGATGGAGTTGTTTCCATTGGGAACTTGGGCTGGCCTCTGGCAAACACTGTTGTTTTCGGTGTGCTTCTGAGTGAGAGTTATAGTGATGAGAAGTTAATGAGTTCTAAAGATTTTCGGAGGGAGTATTTGGTGACTTTCTTATGTACTGTTGTATTGGAGCTGTTCTATTTTGCTGAACTGTCGCTTTGGGGGCTATTGCTTTGTGGTATGTTGCTGTACGTTGCTGTTAGGGAGTTGGATCCTTTTCATGCGAGTTATGTTGAACTGGGAATGGAGTCGTCGGAATCATTTAGTGCATCCGTTATGAAACCTATTAGGCACATTTTGAGTGAGAGGAAATCGCGGAAGATTGCACTTTTCCTTCTGATCAATACTGCATACATGTTTGTGGAATTCATTGCTGGGTTCATGAGCAATAGTCTAGGGCTGATATCAGATGCCTGTCACATGTTGTTTGATTGTGCTGCTCTGGCAATTGGGCTATATGCTTCGTATATTTCGCGCTTGCCTGCAAATAATCAGTTTAACTATGGCCGTGGGAGATTTGAGGTTCTTGCAGGATATACAAATGCTGTTTTCCTGGTTTTGGTTGGAGCATTAATAGTATTGGAATCACTTGAGAGGATTTTGGACCCTCAGGAGATGTCAACTAGCAGTATACTAGTTGTTTCAATTGGAGGGCTTCTTGTCAATGTGGTTGGTTTAATCTTCTTTCATGAGGAGCATCATCATGCCCATGGTGGATCCGGATCATGCTCTCACTCCCACAGTCATCAACATTCGCATGACTCTGTTGGACATAATCATGAAAAGCTTGAGGAATCGTGTATAGCTATTTCTCATGAATGCCATGAAGAATCGTGTGCCAGCCATGATGACCATCACCAACATGGCAGTTCTGTTGGCTGCAAAGATCACCGGCAAGATCACCATGATCACAGCCACCAGCATGACCACAGTCACCAGCATGATCACCATGACCACAGCCACCAGCATGACCACCATGATCACAGCCATCATCATGACCACCATGATCACAGCCATCATCATGACCACCATGGCCACAACCACCAGCATGATCACCATGGCCACAACCACCAGCATGGTGGTTCCGAGCTGAAAAGACTGCCAACCGGTGGAGAAAAATCAACTAAGCAACAGCATCGCCACATTGACCACAACATGGAAGGAATATTTCTGCATGTTTTGGCTGACACCTTAGGGAGTGTCGGAGTTGTTATATCAACACTCTTAATTAAGTACAAGGGATGGCTTGTTGCTGATCCTGCATGCTCGATATTTATTTCTGTCTTGATTATATCTTCAGTTATCCCATTGCTCAGGAACTCTGCAGAAGTCTTGCTCCAAAGAATTCCGAGGGCACATGAGCAGGATTTGAGAAAAGCTCTTAATGATGTTAGGAAGATAAGGGGGATTTCTGGTATTCGGAACTTGCATGTATGGAGTTTCACAAACTCAGATGTAGTGGGTACGCTCCATCTTCATGTTTCCACAGAAGTTGACAAAGGATCTGCAAAAGCGCAGGTGTCGCCCTTATTACATGAAGCTGGAATCAAGGATTTAACGGTGCAGTTGGAATGTGTTGAGGGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000724 GO:0000725 GO:0003674 GO:0003682 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009987 GO:0010035 GO:0010038 GO:0033554 GO:0034641 GO:0042221 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0046483 GO:0046686 GO:0050896 GO:0051716 GO:0071704 GO:0090304 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NAP

Protein Analysis

866

Amino Acids

96.36

Weight (kDa)

7.36

Isoelectric Point (pI)

39.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_efflux	PF01545	447 - 579	3.4e-33	Cation efflux transmembrane domain
Cation_efflux	PF01545	714 - 784	1.5e-13	Cation efflux transmembrane domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0012686)

Species	Orthologous Gene IDs
rosa_chinensis	RchiOBHm_Chr2g0087421 RchiOBHm_Chr3g0447701
rosa_laevigata	RLG00000015865 RLG00000025977
rosa_multiflora	Rmu_ssc0000391.1_g000007
rosa_roxburghii	Rroxscaffold_2G00153550 Rroxscaffold_6G00425880 Rroxscaffold_6G00425900
rosa_rugosa	Rorug01G0475300 Rorug02G0604700
rosa_samantha	Rh2BG028900 Rh2DG028700 Rh3AG004300 Rh3AG004500 Rh3BG003900 Rh3CG004400 Rh3DG004300
rosa_wichuraiana	Rw2G002280 Rw3G000320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2521
Acc36I	ACCTGC	1 cut(s)	2521
AccB1I	GGYRCC	1 cut(s)	551
AccB7I	CCANNNNNTGG	2 cut(s)	2123, 2170
AccBSI	CCGCTC	1 cut(s)	291
AccI	GTMKAC	1 cut(s)	1645
AccII	CGCG	3 cut(s)	491, 1330, 1489
AccIII	TCCGGA	1 cut(s)	1733
AclWI	GGATC	9 cut(s)	559, 729, 1220, 1233, 1725, 1738, 1744, 2264, 2524
AcoI	YGGCCR	5 cut(s)	3, 809, 1519, 2047, 2074
AcsI	RAATTY	3 cut(s)	804, 1379, 2358
AcuI	CTGAAG	1 cut(s)	2295
AfaI	GTAC	5 cut(s)	545, 1132, 1205, 2249, 2479
AfiI	CCNNNNNNNGG	3 cut(s)	1573, 2123, 2170
AflIII	ACRYGT	2 cut(s)	1368, 1434
AgeI	ACCGGT	1 cut(s)	2120
AgsI	TTSAA	4 cut(s)	731, 977, 1250, 1659
AhdI	GACNNNNNGTC	1 cut(s)	485
AhlI	ACTAGT	1 cut(s)	1648
AjnI	CCWGG	1 cut(s)	1566
Alw21I	GWGCWC	1 cut(s)	662
Alw26I	GTCTC	2 cut(s)	337, 405
AlwI	GGATC	9 cut(s)	559, 729, 1220, 1233, 1725, 1738, 1744, 2264, 2524
Aor13HI	TCCGGA	1 cut(s)	1733
AoxI	GGCC	6 cut(s)	3, 809, 1019, 1519, 2047, 2074
ApeKI	GCWGC	3 cut(s)	817, 1451, 1893
ApoI	RAATTY	3 cut(s)	804, 1379, 2358
AseI	ATTAAT	1 cut(s)	1586
AsiGI	ACCGGT	1 cut(s)	2120
Asp700I	GAANNNNTTC	1 cut(s)	735
AspLEI	GCGC	2 cut(s)	1491, 2530
AspS9I	GGNCC	1 cut(s)	1618
AvaII	GGWCC	1 cut(s)	1618
AxyI	CCTNAGG	2 cut(s)	1623, 2206
BaeGI	GKGCMC	1 cut(s)	2371
BalI	TGGCCA	3 cut(s)	811, 2049, 2076
BamHI	GGATCC	2 cut(s)	1225, 1730
BanI	GGYRCC	1 cut(s)	551
BanII	GRGCYC	1 cut(s)	662
BbsI	GAAGAC	1 cut(s)	508
Bbv12I	GWGCWC	1 cut(s)	662
BbvCI	CCTCAGC	1 cut(s)	981
BbvI	GCAGC	3 cut(s)	804, 1438, 1880
BccI	CCATC	8 cut(s)	24, 449, 983, 1875, 2010, 2037, 2250, 2493
BceAI	ACGGC	1 cut(s)	1506
BcgI	CGANNNNNNTGC	2 cut(s)	2132, 2166
BciT130I	CCWGG	1 cut(s)	1568
BclI	TGATCA	6 cut(s)	1353, 1921, 1957, 1993, 2020, 2065
BcoDI	GTCTC	2 cut(s)	337, 405
BcuI	ACTAGT	1 cut(s)	1648
BfaI	CTAG	6 cut(s)	359, 569, 786, 1409, 1638, 1649
BfmI	CTRYAG	1 cut(s)	2337
BfuAI	ACCTGC	1 cut(s)	2521
Bme1390I	CCNGG	1 cut(s)	1568
Bme18I	GGWCC	1 cut(s)	1618
BmeRI	GACNNNNNGTC	1 cut(s)	485
BmgT120I	GGNCC	1 cut(s)	1618
BmiI	GGNNCC	5 cut(s)	553, 1227, 1620, 1732, 2098
BmrFI	CCNGG	1 cut(s)	1568
BmrI	ACTGGG	2 cut(s)	702, 1262
BmsI	GCATC	4 cut(s)	1292, 1414, 1720, 2158
BmuI	ACTGGG	2 cut(s)	702, 1262
BoxI	GACNNNNGTC	1 cut(s)	1944
BpiI	GAAGAC	1 cut(s)	508
BplI	GAGNNNNNCTC	4 cut(s)	504, 536, 2377, 2409
BpmI	CTGGAG	1 cut(s)	391
Bpu10I	CCTNAGC	2 cut(s)	981, 2327
BpuEI	CTTGAG	2 cut(s)	1625, 1823
Bsa29I	ATCGAT	1 cut(s)	497
BsaBI	GATNNNNATC	3 cut(s)	651, 1735, 1866
BsaI	GGTCTC	1 cut(s)	405
BsaJI	CCNNGG	6 cut(s)	663, 1522, 1723, 2044, 2071, 2363
BsaWI	WCCGGW	3 cut(s)	540, 1733, 2120
BsaXI	ACNNNNNCTCC	6 cut(s)	26, 56, 762, 792, 978, 1008
Bsc4I	CCNNNNNNNGG	3 cut(s)	1573, 2123, 2170
Bse118I	RCCGGY	2 cut(s)	1905, 2120
Bse1I	ACTGG	2 cut(s)	697, 1257
Bse21I	CCTNAGG	2 cut(s)	1623, 2206
Bse3DI	GCAATG	2 cut(s)	1383, 2321
Bse8I	GATNNNNATC	3 cut(s)	651, 1735, 1866
BseAI	TCCGGA	1 cut(s)	1733
BseBI	CCWGG	1 cut(s)	1568
BseCI	ATCGAT	1 cut(s)	497
BseDI	CCNNGG	6 cut(s)	663, 1522, 1723, 2044, 2071, 2363
BseGI	GGATG	2 cut(s)	1283, 2261
BseJI	GATNNNNATC	3 cut(s)	651, 1735, 1866
BseLI	CCNNNNNNNGG	3 cut(s)	1573, 2123, 2170
BseMI	GCAATG	2 cut(s)	1383, 2321
BseMII	CTCAG	5 cut(s)	972, 1046, 1637, 2341, 2478
BseNI	ACTGG	2 cut(s)	697, 1257
BseSI	GKGCMC	1 cut(s)	2371
BseXI	GCAGC	3 cut(s)	804, 1438, 1880
BseYI	CCCAGC	3 cut(s)	471, 717, 1388
BsgI	GTGCAG	1 cut(s)	2597
Bsh1236I	CGCG	3 cut(s)	491, 1330, 1489
BshFI	GGCC	6 cut(s)	5, 811, 1021, 1521, 2049, 2076
BshNI	GGYRCC	1 cut(s)	551
BshTI	ACCGGT	1 cut(s)	2120
BshVI	ATCGAT	1 cut(s)	497
BsiHKAI	GWGCWC	1 cut(s)	662
BsiSI	CCGG	5 cut(s)	510, 541, 1734, 1906, 2121
BslFI	GGGAC	1 cut(s)	889
BslI	CCNNNNNNNGG	3 cut(s)	1573, 2123, 2170
BsmAI	GTCTC	2 cut(s)	337, 405
BsmBI	CGTCTC	1 cut(s)	337
BsmFI	GGGAC	1 cut(s)	889
BsmI	GAATGC	2 cut(s)	737, 1838
BsnI	GGCC	6 cut(s)	5, 811, 1021, 1521, 2049, 2076
Bso31I	GGTCTC	1 cut(s)	405
Bsp1286I	GDGCHC	2 cut(s)	662, 2371
Bsp13I	TCCGGA	1 cut(s)	1733
Bsp19I	CCATGG	3 cut(s)	1723, 2044, 2071
Bsp68I	TCGCGA	1 cut(s)	491
BspANI	GGCC	6 cut(s)	5, 811, 1021, 1521, 2049, 2076
BspCNI	CTCAG	5 cut(s)	973, 1047, 1636, 2340, 2477
BspDI	ATCGAT	1 cut(s)	497
BspEI	TCCGGA	1 cut(s)	1733
BspFNI	CGCG	3 cut(s)	491, 1330, 1489
BspHI	TCATGA	6 cut(s)	1395, 1702, 1792, 1828, 2008, 2035
BspLI	GGNNCC	5 cut(s)	553, 1227, 1620, 1732, 2098
BspMAI	CTGCAG	1 cut(s)	2341
BspMI	ACCTGC	1 cut(s)	2521
BspPI	GGATC	9 cut(s)	559, 729, 1220, 1233, 1725, 1738, 1744, 2264, 2524
BspT107I	GGYRCC	1 cut(s)	551
BspTNI	GGTCTC	1 cut(s)	405
BsrBI	CCGCTC	1 cut(s)	291
BsrDI	GCAATG	2 cut(s)	1383, 2321
BsrFI	RCCGGY	2 cut(s)	1905, 2120
BsrI	ACTGG	2 cut(s)	697, 1257
BssAI	RCCGGY	2 cut(s)	1905, 2120
BssECI	CCNNGG	6 cut(s)	663, 1522, 1723, 2044, 2071, 2363
BssNAI	GTATAC	1 cut(s)	1646
BssT1I	CCWWGG	4 cut(s)	663, 1723, 2044, 2071
Bst1107I	GTATAC	1 cut(s)	1646
Bst2UI	CCWGG	1 cut(s)	1568
Bst4CI	ACNGT	7 cut(s)	934, 1036, 1135, 1167, 1757, 1946, 2575
Bst6I	CTCTTC	5 cut(s)	169, 242, 317, 377, 893
BstC8I	GCNNGC	5 cut(s)	1019, 1493, 1497, 1855, 2278
BstDEI	CTNAG	8 cut(s)	750, 981, 1055, 1623, 2139, 2206, 2327, 2464
BstDSI	CCRYGG	4 cut(s)	1522, 1723, 2044, 2071
BstF5I	GGATG	2 cut(s)	1283, 2261
BstFNI	CGCG	3 cut(s)	491, 1330, 1489
BstHHI	GCGC	2 cut(s)	1491, 2530
BstMAI	GTCTC	2 cut(s)	337, 405
BstNI	CCWGG	1 cut(s)	1568
BstNSI	RCATGY	5 cut(s)	1372, 1438, 2192, 2280, 2447
BstPAI	GACNNNNGTC	1 cut(s)	1944
BstSCI	CCNGG	1 cut(s)	1566
BstSFI	CTRYAG	1 cut(s)	2337
BstSLI	GKGCMC	1 cut(s)	2371
BstUI	CGCG	3 cut(s)	491, 1330, 1489
BstV1I	GCAGC	3 cut(s)	804, 1438, 1880
BstV2I	GAAGAC	1 cut(s)	508
BstX2I	RGATCY	3 cut(s)	1225, 1730, 2516
BstXI	CCANNNNNNTGG	2 cut(s)	2090, 2093
BstYI	RGATCY	3 cut(s)	1225, 1730, 2516
BstZ17I	GTATAC	1 cut(s)	1646
Bsu15I	ATCGAT	1 cut(s)	497
Bsu36I	CCTNAGG	2 cut(s)	1623, 2206
BsuRI	GGCC	6 cut(s)	5, 811, 1021, 1521, 2049, 2076
BsuTUI	ATCGAT	1 cut(s)	497
BtgI	CCRYGG	4 cut(s)	1522, 1723, 2044, 2071
BtgZI	GCGATG	1 cut(s)	2135
BtsCI	GGATG	2 cut(s)	1283, 2261
BtsI	GCAGTG	1 cut(s)	646
BtsIMutI	CAGTG	3 cut(s)	646, 690, 1032
BtuMI	TCGCGA	1 cut(s)	491
BveI	ACCTGC	1 cut(s)	2521
Cac8I	GCNNGC	5 cut(s)	1019, 1493, 1497, 1855, 2278
CciI	TCATGA	6 cut(s)	1395, 1702, 1792, 1828, 2008, 2035
CfoI	GCGC	2 cut(s)	1491, 2530
Cfr10I	RCCGGY	2 cut(s)	1905, 2120
Cfr13I	GGNCC	1 cut(s)	1618
ClaI	ATCGAT	1 cut(s)	497
Csp6I	GTAC	5 cut(s)	544, 1131, 1204, 2248, 2478
CspAI	ACCGGT	1 cut(s)	2120
CspCI	CAANNNNNGTGG	4 cut(s)	652, 687, 2488, 2523
CviQI	GTAC	5 cut(s)	544, 1131, 1204, 2248, 2478
DdeI	CTNAG	8 cut(s)	750, 981, 1055, 1623, 2139, 2206, 2327, 2464
DriI	GACNNNNNGTC	1 cut(s)	485
EaeI	YGGCCR	5 cut(s)	3, 809, 1519, 2047, 2074
Eam1104I	CTCTTC	5 cut(s)	169, 242, 317, 377, 893
Eam1105I	GACNNNNNGTC	1 cut(s)	485
EarI	CTCTTC	5 cut(s)	169, 242, 317, 377, 893
EciI	GGCGGA	6 cut(s)	124, 181, 373, 379, 385, 623
Ecl136II	GAGCTC	1 cut(s)	660
Eco130I	CCWWGG	4 cut(s)	663, 1723, 2044, 2071
Eco24I	GRGCYC	1 cut(s)	662
Eco31I	GGTCTC	1 cut(s)	405
Eco32I	GATATC	1 cut(s)	1419
Eco47I	GGWCC	1 cut(s)	1618
Eco53kI	GAGCTC	1 cut(s)	660
Eco57I	CTGAAG	1 cut(s)	2295
Eco81I	CCTNAGG	2 cut(s)	1623, 2206
EcoICRI	GAGCTC	1 cut(s)	660
EcoRI	GAATTC	2 cut(s)	1379, 2358
EcoRII	CCWGG	1 cut(s)	1566
EcoRV	GATATC	1 cut(s)	1419
EcoT14I	CCWWGG	4 cut(s)	663, 1723, 2044, 2071
EcoT38I	GRGCYC	1 cut(s)	662
ErhI	CCWWGG	4 cut(s)	663, 1723, 2044, 2071
Esp3I	CGTCTC	1 cut(s)	337
FaqI	GGGAC	1 cut(s)	889
FauI	CCCGC	1 cut(s)	110
FbaI	TGATCA	6 cut(s)	1353, 1921, 1957, 1993, 2020, 2065
FblI	GTMKAC	1 cut(s)	1645
FokI	GGATG	2 cut(s)	1270, 2268
FriOI	GRGCYC	1 cut(s)	662
FspBI	CTAG	6 cut(s)	359, 569, 786, 1409, 1638, 1649
GlaI	GCGC	2 cut(s)	1490, 2529
GsaI	CCCAGC	3 cut(s)	475, 721, 1392
GsuI	CTGGAG	1 cut(s)	391
HaeIII	GGCC	6 cut(s)	5, 811, 1021, 1521, 2049, 2076
HapII	CCGG	5 cut(s)	510, 541, 1734, 1906, 2121
HhaI	GCGC	2 cut(s)	1491, 2530
Hin6I	GCGC	2 cut(s)	1489, 2528
HinP1I	GCGC	2 cut(s)	1489, 2528
HincII	GTYRAC	2 cut(s)	1635, 2509
HindII	GTYRAC	2 cut(s)	1635, 2509
HindIII	AAGCTT	1 cut(s)	1799
HpaII	CCGG	5 cut(s)	510, 541, 1734, 1906, 2121
Hpy166II	GTNNAC	4 cut(s)	690, 1635, 1646, 2509
Hpy8I	GTNNAC	4 cut(s)	690, 1635, 1646, 2509
Hpy99I	CGWCG	2 cut(s)	491, 1270
HpyCH4III	ACNGT	7 cut(s)	934, 1036, 1135, 1167, 1757, 1946, 2575
HpyCH4IV	ACGT	1 cut(s)	1206
HpyF3I	CTNAG	8 cut(s)	750, 981, 1055, 1623, 2139, 2206, 2327, 2464
HpySE526I	ACGT	1 cut(s)	1206
HspAI	GCGC	2 cut(s)	1489, 2528
Kpn2I	TCCGGA	1 cut(s)	1733
Ksp22I	TGATCA	6 cut(s)	1353, 1921, 1957, 1993, 2020, 2065
LmnI	GCTCC	8 cut(s)	296, 657, 665, 1144, 1580, 1708, 2355, 2487
Lsp1109I	GCAGC	3 cut(s)	804, 1438, 1880
LweI	GCATC	4 cut(s)	1292, 1414, 1720, 2158
MaeI	CTAG	6 cut(s)	359, 569, 786, 1409, 1638, 1649
MaeII	ACGT	1 cut(s)	1206
MaeIII	GTNAC	3 cut(s)	1117, 1430, 1946
MbiI	CCGCTC	1 cut(s)	291
MfeI	CAATTG	2 cut(s)	1461, 1659
MflI	RGATCY	3 cut(s)	1225, 1730, 2516
MhlI	GDGCHC	2 cut(s)	662, 2371
MlsI	TGGCCA	3 cut(s)	811, 2049, 2076
MluCI	AATT	7 cut(s)	804, 868, 1379, 1461, 1659, 2241, 2358
MluNI	TGGCCA	3 cut(s)	811, 2049, 2076
MlyI	GAGTC	4 cut(s)	474, 522, 1271, 1769
MmeI	TCCRAC	6 cut(s)	1203, 1248, 1558, 1762, 2197, 2562
Mox20I	TGGCCA	3 cut(s)	811, 2049, 2076
MroI	TCCGGA	1 cut(s)	1733
MroXI	GAANNNNTTC	1 cut(s)	735
MscI	TGGCCA	3 cut(s)	811, 2049, 2076
MseI	TTAA	8 cut(s)	1082, 1512, 1586, 1691, 2240, 2244, 2397, 2570
Msp20I	TGGCCA	3 cut(s)	811, 2049, 2076
MspA1I	CMGCKG	1 cut(s)	209
MspI	CCGG	5 cut(s)	510, 541, 1734, 1906, 2121
MspR9I	CCNGG	1 cut(s)	1568
MunI	CAATTG	2 cut(s)	1461, 1659
Mva1269I	GAATGC	2 cut(s)	737, 1838
MvaI	CCWGG	1 cut(s)	1568
MvnI	CGCG	3 cut(s)	491, 1330, 1489
NcoI	CCATGG	3 cut(s)	1723, 2044, 2071
NlaIV	GGNNCC	5 cut(s)	553, 1227, 1620, 1732, 2098
NmeAIII	GCCGAG	1 cut(s)	598
NmuCI	GTSAC	3 cut(s)	1117, 1430, 1946
NruI	TCGCGA	1 cut(s)	491
NspI	RCATGY	5 cut(s)	1372, 1438, 2192, 2280, 2447
PacI	TTAATTAA	1 cut(s)	2244
PaeI	GCATGC	1 cut(s)	2280
PagI	TCATGA	6 cut(s)	1395, 1702, 1792, 1828, 2008, 2035
PaqCI	CACCTGC	1 cut(s)	2521
PciI	ACATGT	2 cut(s)	1368, 1434
PctI	GAATGC	2 cut(s)	737, 1838
PdmI	GAANNNNTTC	1 cut(s)	735
PfeI	GAWTC	7 cut(s)	499, 652, 1271, 1598, 1808, 1844, 2559
PflMI	CCANNNNNTGG	2 cut(s)	2123, 2170
PinAI	ACCGGT	1 cut(s)	2120
PleI	GAGTC	4 cut(s)	474, 521, 1270, 1769
PpsI	GAGTC	4 cut(s)	474, 521, 1270, 1769
PscI	ACATGT	2 cut(s)	1368, 1434
PshAI	GACNNNNGTC	1 cut(s)	1944
PshBI	ATTAAT	1 cut(s)	1586
Psp124BI	GAGCTC	1 cut(s)	662
Psp6I	CCWGG	1 cut(s)	1566
PspFI	CCCAGC	3 cut(s)	471, 717, 1388
PspGI	CCWGG	1 cut(s)	1566
PspN4I	GGNNCC	5 cut(s)	553, 1227, 1620, 1732, 2098
PspPI	GGNCC	1 cut(s)	1618
PstI	CTGCAG	1 cut(s)	2341
PsuI	RGATCY	3 cut(s)	1225, 1730, 2516
RruI	TCGCGA	1 cut(s)	491
RsaI	GTAC	5 cut(s)	545, 1132, 1205, 2249, 2479
RsaNI	GTAC	5 cut(s)	544, 1131, 1204, 2248, 2478
SacI	GAGCTC	1 cut(s)	662
SaqAI	TTAA	8 cut(s)	1082, 1512, 1586, 1691, 2240, 2244, 2397, 2570
Sau96I	GGNCC	1 cut(s)	1618
SchI	GAGTC	4 cut(s)	474, 522, 1271, 1769
ScrFI	CCNGG	1 cut(s)	1568
SduI	GDGCHC	2 cut(s)	662, 2371
SfaNI	GCATC	4 cut(s)	1292, 1414, 1720, 2158
SfcI	CTRYAG	1 cut(s)	2337
SinI	GGWCC	1 cut(s)	1618
SmlI	CTYRAG	2 cut(s)	1604, 1802
SmoI	CTYRAG	2 cut(s)	1604, 1802
SpeI	ACTAGT	1 cut(s)	1648
SphI	GCATGC	1 cut(s)	2280
Sse9I	AATT	7 cut(s)	804, 868, 1379, 1461, 1659, 2241, 2358
SspI	AATATT	1 cut(s)	2181
SspMI	CTAG	6 cut(s)	359, 569, 786, 1409, 1638, 1649
SstI	GAGCTC	1 cut(s)	662
StyD4I	CCNGG	1 cut(s)	1566
StyI	CCWWGG	4 cut(s)	663, 1723, 2044, 2071
TaaI	ACNGT	7 cut(s)	934, 1036, 1135, 1167, 1757, 1946, 2575
TaiI	ACGT	1 cut(s)	1209
TaqI	TCGA	3 cut(s)	497, 863, 2282
TasI	AATT	7 cut(s)	804, 868, 1379, 1461, 1659, 2241, 2358
TatI	WGTACW	2 cut(s)	1130, 2247
TfiI	GAWTC	7 cut(s)	499, 652, 1271, 1598, 1808, 1844, 2559
Tru1I	TTAA	8 cut(s)	1082, 1512, 1586, 1691, 2240, 2244, 2397, 2570
Tru9I	TTAA	8 cut(s)	1082, 1512, 1586, 1691, 2240, 2244, 2397, 2570
TscAI	CASTG	3 cut(s)	646, 697, 1039
TseFI	GTSAC	3 cut(s)	1117, 1430, 1946
TseI	GCWGC	3 cut(s)	817, 1451, 1893
Tsp45I	GTSAC	3 cut(s)	1117, 1430, 1946
TspGWI	ACGGA	3 cut(s)	729, 813, 1276
TspRI	CASTG	3 cut(s)	646, 697, 1039
Van91I	CCANNNNNTGG	2 cut(s)	2123, 2170
VpaK11BI	GGWCC	1 cut(s)	1618
VspI	ATTAAT	1 cut(s)	1586
XapI	RAATTY	3 cut(s)	804, 1379, 2358
XceI	RCATGY	5 cut(s)	1372, 1438, 2192, 2280, 2447
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1009, 1874, 2090
XmiI	GTMKAC	1 cut(s)	1645
XmnI	GAANNNNTTC	1 cut(s)	735
XspI	CTAG	6 cut(s)	359, 569, 786, 1409, 1638, 1649

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.