Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000003

Orthogroup ID

OG0003078

Physical Location & Seq

Genomic Coordinates Forward (+)

21261721 .. 21270996

Gene Structure 9276 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug03G0230700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4113 bp

ATGCCAACTCTGTTCCTCCATTTCTTCCTTTTCTTATTCACTACCACTGTTACTAATCTCATCCCCGCAGTTCACAGCAACTGCATTGAGGCCCAGAAACAATCATTGCTCCATTTCAAACAAAGTCTTGAATTTAATTCTTCCGAATCCTCCAAGCTTGTCACATGGAATTCGAGTACCGAATGTTGTTCTTGGTTGGGTGTAAGTTGCAGCACCAATGGGAGTGTTGTCGGACTTAACCTCAGCAGCGAGTCTATCTCATGCAGCATTGACAATTCAAGTAGTCTCTTCCAACTTCAACATCTTCAGAGCCTCAATTTGGCCGACAACAACTTCGAAGGCTCTTTAATTCCATCTGCAATCGGAAAGCTTGCAAATTTGAGGTATTTGAATTTACAAGAAGCTAATTTTTCTGGGCAAATTCCCATTGAGGTCGCACGCTTGAATAGGTTGAAAGTTCTTGATCTGTCTAGAAGTTACCTTTTGGGGGGATTAAAACTTGAGAGCCCGAATTTAGGCACTTTAATCCAGAACCTTACAGAGCTTACAGAGTTAATATTGGATGGAAACTACATCCCTGGTCTGGTTCCAAAATTCTTAGCCAATTTTTCAAGTTTGATTTCCTTGAGTCTATCTGCTTGTAACTTGTACGGAGCATTTCCCAAAGAGATATTCCTGGTACCGACACTAAAGTCTGTTAACCTGTCACTTAATTCACAGCTTCGTGGTTCCTTACCGGAGTTTCCGAAGAATGGATCTCTCGAATCCCTAGTTCTATCCCAGACTAAATTTTCAGGAGTCTTGCCGGACTCTATTGGCAACCTTAAAAAGCTGTCTGAATTAGATCTTTCAGAGTGCAATTTCACAGGAGCAGTTCCAAAGTCACTGGAAAACCTAACACAATTGGGGGATTTGGCCTTGTCATACAACAAGTTTTCTGGTCCGATTAATTCCATTCAATGGGAAAAACTCATCAAGCTTGACAGTCTCGACTTGGCCAACAATCTACTCTACGGGAGTATTCCATTGTCTCCCTTTTCTATTCCCTTGCTGCAGAGGTTAGATCTTTCTGGAAATAACTTCTCTGGTCAATTGCTTGAATTTTCTAATGTCTCTTCGCACTTGCTAGAAGAACTTGATTTGAGTAGCAACAATTTGGAAGGATCAGTACCCGTGTCTATCTTTAATCTTCCAGGGCTTCAGGAGTTTAATCTTTCTTCAAACAACTTGAGTGGTTTTCCTTTTTATGGCCTTCCGGAGTCGAGAAATCTTTCTTCTCTTGATCTATCCTTCAATAGCTTGTTGTTCAATCATAATGGTACTAACTCATCACACCCCTCCCTCCTTCAGATTAAAACCCTGAAGTTGGCTTCTAACAAGTTGAAAACATTCCCAAATTTCTTGAAAAATCTATCTTATTTAACCGACTTGGACCTTTCAGACAACCAAATTCCAGGTCAGATACCCAATTGGATTTGGGAGCTTCATCTCGAGAATCTATATCTATGTTGTAACAAGTTGGTAAGTCTAGAAGCTCCTTTACCTGGTTCTACTTACCATCTGATGATTCTTGATCTTCACTCAAACCAGATTCAGGGACAAATACCATTGTTTCTAAGTGGTAATTATTTGGATTACTCAGGAAATAATTTCAGCTCTAGTATGCCAATTGACATTGGTGACATCACCAATTCCACGGGCTACCTTTCTCTTTCAAGCAGTAACTTGTATGGGGAAATTCCAAAATCAATATGCAGTGCAAGTAATGTAATTCTTGATCTCTCTAATAACTCTTTGAGAGGAACCATTCCCCAATGCTTGACTCAGACTTCTCTTTCTGTCCTTGATTTGAGGAGAAACAACCTTACTGGATCTATCCCTGATACTTTTCTTGAAGGTTGTCCTTTGGTTACTCTAGCTCTGGGCAGAAATCAGATACAAGGTCAATTTCCAAAATCTCTAGCCAATTGTTCAAGCTTAGAGGTTTTAGACCTTGGAATTAATCAAATAACAGATGCCTTTCCCTGCCCGTTGAAGACCATATCCACCTTGCGTGTCCTTGTGTTGCGATCCAACAAATTTTATGGACCTATTGGATGTCCCGAGACCAAAGACACCTGGCTGAAGCTTCAAATCATAAACTTGGCTAATAACAGTTTCAATGGTGAAATACCAGGTACATCATTGACAACATGGCAGGCAATGGTGGTTAATGAAGATGGTTACCAAATTCACCATAGCTATAAGTGGGTACCACCAGGTAGTGAAATTGTGTCCTCTGCTGGAACTGCGGTCAGTTGTGGACGAGGCAGCACTGGAACTCCATGCCATAACTCATCACCACGTAGTGCAATTACGTTGAATGCTCCAACTGCGGTCAGTTGTTATCGAAAGAACACTGGAACTCCATGCCATAACTCACCACCTAGGTATTACTCACCACCTAATGAGAGCTCTATTATTGATTATGCAGATAGAGTAACAACTACAAGCAAAGGTCAAGAGATGGAGCTTGTATATATTTCATTTTTCTTCACTTCGATTGACTTCTCTGCTAACAAGTTCAACGGAACAATACCCATGGAAATGGGCCAACTGAGATCATTATATGCCCTCAATCTGTCCAGTAATGCTTTCACAGGCGAAATCCCATCATCCTTTGGTAACATGAGAAGCATAGAGTCCTTAGACCTCTCACAGAACCACCTGAGCGGCCAAATTCCACCGCAGCTTGCAACTCTCACTTTCCTTTCGTACTTGAATGTCTCATACAATCAACTGACGGGAAGGATTCCACCCAGCACTCAATTTTCGACATTTCCAAATACCTCCTTCGAGGGTAATAAAGGATTATGGGGGCCTCCTCTGACATGGGATACAGCAATCGTATTGCCACCTCCAAAGTTTAATGGAAGCTCAAGTAATCCGAATTCTGGAGATGAGATTGATTGGAATGTTATCAGTGCTGAAATTGGATTTACATGTGGGTTTGGAATTGCCGTCGGGTCACTTTTGTTTTGCAAGAGATGGAGGAAATGGTACTACAGAGCTATGTTTAACATCCTGTTCAAGATATTCCCTCAGCTGGAACACAGATTTGGTAATCACAGAAGGCATGTTTACATAAATCAAAGTTCATCTCCTCTATACATTTTCCAAGGACCGTCTGAGTTTATGCCACCAGAAAAAGGCTTGAAAAGTTTTTTAAGAGAGTACAAGATGCGTGAAGATATTTTAGATCAGCAGGCATCACTTCAACGAGCAAGACTTGAAGCTTCTCTTGCTGATGGCATCTCTTGGGGTATGGGAGAGGATGCCATTATAGAAGAAGCTGAGGATGATGGTGAGGAAGTAACTTGGCAAACATACAAAGGACAGCTTTCGGAAAAGCAGATAAAAACCCGTGATAAAATAACTAAAAGAATGGAAAAGATTTCTGATATGAAGAAAGAGATAGATGCTATTCGTGCTAAAGACGTCTCTCAAGGTGGATTGACTCAAGGGCAACAAACTCAGATCGCGCGAAATGGACAAAGAACAGAACAGATTCTGGTAGAGCTTGAAAATTTGGAAGAGACGCTGAATGAGAGTATTCGAGAAAGTTTAGGTGCACGTGTTGGGAAGCTATCTCATGGTAAGAAGAAAGGAGCAATTGAAGAAGAAGAAGAACTTTTGAGAGTGGAAGAAATATCTCTTATATTCAAGAACTTGCATCATCTTCTGAACTCATTGCGGCCCCATCAGGCTAGGGGAACACTTGTTCACATTCTAGAACTTCAGATAAAACGACGTAAACAAGCTGTGGAGGATGTAAAGAGGAGGAGAGAAGAAGCGCAGGCACTTCTGAAGGAGTCCCTTGAATCGTTGGAGGACACTGGTCCATCTCTTATGTTGAAATTGGGAGGGCTCATATATGTTCCTACTCAATTATCAGAGCCCCTCAGACAGTTTCATCTAATACCTTTAGGGAGGGTCATGGAATCCCACTGGTCAAATTTTCTCGGACAGACAGTTGTATCTAGTACCGTCATGCTTTTATATCAGAAACTTCTGGTTTTACCTTTCTATTCTAGTGTTGAGAGGAAAGTAGTCCATCTAGCTGTTATTGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001676 GO:0003674 GO:0003824 GO:0004467 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006082 GO:0006629 GO:0006631 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010025 GO:0010143 GO:0010166 GO:0012505 GO:0015645 GO:0016405 GO:0016874 GO:0016877 GO:0016878 GO:0019752 GO:0031957 GO:0032787 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044255 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0071704 GO:1901568 GO:1901570 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00061 ko00071 ko01100 ko01212 ko02024 ko03320 ko04146 ko04216 ko04714 ko04920 map00061 map00071 map01100 map01212 map02024 map03320 map04146 map04216 map04714 map04920

Pfam Domains

Protein Families

AMP-binding

Protein Analysis

1370

Amino Acids

151.86

Weight (kDa)

5.93

Isoelectric Point (pI)

43.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	31 - 71	4.5e-09	Leucine rich repeat N-terminal domain
LRR_14	PF23598	85 - 235	3.2e-11	Leucine-rich repeat region
LRR_8	PF13855	253 - 312	3.3e-06	Leucine rich repeat
LRR_8	PF13855	376 - 434	6.6e-07	Leucine rich repeat
LRR_8	PF13855	867 - 927	6.5e-08	Leucine rich repeat
Med7	PF05983	1223 - 1282	6.1e-15	MED7 protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003078)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47240 AT2G47240 AT2G47240 AT2G47240 AT2G47245
fragaria_vesca	FvH4_1g26810 FvH4_1g26810 FvH4_1g26810 FvH4_1g26850 FvH4_1g26850 FvH4_1g26850
malus_domestica	MD09G1286100.v1.1
prunus_persica	Prupe.3G007200_v2.0.a1
pyrus_communis	pycom09g19340
rosa_chinensis	RchiOBHm_Chr3g0487031 RchiOBHm_Chr3g0487091
rosa_laevigata	RLG00000023034 RLG00000023036
rosa_multiflora	Rmu_sc0005856.1_g000028
rosa_roxburghii	Rroxscaffold_6G00395190 Rroxscaffold_6G00395220
rosa_rugosa	Rorug03G0230300 Rorug03G0230500 Rorug03G0230600 Rorug03G0230700 Rorug03G0230800 Rorug03G0230900
rosa_samantha	Rh3AG278900 Rh3AG279200 Rh3BG315100 Rh3CG313100 Rh3CG313200 Rh3DG309800 Rh3DG310100
rosa_wichuraiana	Rw0G017060 Rw3G024720 Rw3G024750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	691
AatII	GACGTC	1 cut(s)	3480
Acc65I	GGTACC	2 cut(s)	679, 2251
AccB1I	GGYRCC	2 cut(s)	679, 2251
AccB7I	CCANNNNNTGG	1 cut(s)	960
AccBSI	CCGCTC	1 cut(s)	2712
AccII	CGCG	2 cut(s)	3521, 3523
AccIII	TCCGGA	1 cut(s)	1255
AciI	CCGC	6 cut(s)	66, 2291, 2375, 2712, 2726, 3733
AclWI	GGATC	4 cut(s)	763, 1171, 1879, 2064
AcoI	YGGCCR	3 cut(s)	321, 996, 2713
AcuI	CTGAAG	7 cut(s)	290, 1184, 1331, 1382, 2144, 3761, 3866
AcvI	CACGTG	1 cut(s)	3614
AcyI	GRCGYC	1 cut(s)	3477
AdeI	CACNNNGTG	1 cut(s)	2348
AflIII	ACRYGT	2 cut(s)	2981, 3613
AjnI	CCWGG	8 cut(s)	577, 675, 1192, 1453, 1543, 2117, 2173, 2257
AjuI	GAANNNNNNNTTGG	4 cut(s)	656, 688, 3081, 3113
AloI	GAACNNNNNNTCC	2 cut(s)	3744, 3776
Alw21I	GWGCWC	2 cut(s)	2456, 3613
Alw26I	GTCTC	8 cut(s)	290, 992, 1035, 1117, 2099, 2770, 3484, 3569
Alw44I	GTGCAC	1 cut(s)	3609
AlwI	GGATC	4 cut(s)	763, 1171, 1879, 2064
AlwNI	CAGNNNCTG	2 cut(s)	81, 3550
Ama87I	CYCGRG	2 cut(s)	1490, 2102
Aor13HI	TCCGGA	1 cut(s)	1255
AoxI	GGCC	9 cut(s)	90, 321, 915, 996, 1249, 2590, 2713, 2858, 3734
ApaLI	GTGCAC	1 cut(s)	3609
ApeKI	GCWGC	6 cut(s)	210, 246, 264, 1051, 2310, 2728
ArsI	GACNNNNNNTTYG	4 cut(s)	317, 349, 1441, 1473
AseI	ATTAAT	2 cut(s)	948, 2001
Asp700I	GAANNNNTTC	3 cut(s)	1270, 1388, 3546
Asp718I	GGTACC	2 cut(s)	679, 2251
AspA2I	CCTAGG	1 cut(s)	2426
AspLEI	GCGC	2 cut(s)	3523, 3835
AspS9I	GGNCC	9 cut(s)	91, 941, 1432, 2087, 2590, 2858, 3161, 3735, 3878
AsuHPI	GGTGA	8 cut(s)	1678, 1691, 2177, 2225, 2331, 2412, 2430, 3356
AsuII	TTCGAA	1 cut(s)	336
AvaI	CYCGRG	2 cut(s)	1490, 2102
AvaII	GGWCC	5 cut(s)	941, 1432, 2087, 3161, 3878
AvrII	CCTAGG	1 cut(s)	2426
BaeGI	GKGCMC	1 cut(s)	3613
BaeI	ACNNNNGTAYC	2 cut(s)	3999, 4032
BalI	TGGCCA	1 cut(s)	998
BanI	GGYRCC	2 cut(s)	679, 2251
BanII	GRGCYC	4 cut(s)	509, 2456, 3909, 3939
BarI	GAAGNNNNNNTAC	4 cut(s)	1152, 1184, 1524, 1556
BbrPI	CACGTG	1 cut(s)	3614
BbsI	GAAGAC	1 cut(s)	2042
Bbv12I	GWGCWC	2 cut(s)	2456, 3613
BbvCI	CCTCAGC	3 cut(s)	242, 3081, 3333
BbvI	GCAGC	6 cut(s)	222, 258, 276, 1038, 2322, 2740
BceAI	ACGGC	1 cut(s)	2984
BciT130I	CCWGG	8 cut(s)	579, 677, 1194, 1455, 1545, 2119, 2175, 2259
BciVI	GTATCC	1 cut(s)	2869
BcoDI	GTCTC	8 cut(s)	290, 992, 1035, 1117, 2099, 2770, 3484, 3569
BfmI	CTRYAG	2 cut(s)	1052, 3043
BfuI	GTATCC	1 cut(s)	2869
BglII	AGATCT	2 cut(s)	844, 1063
BisI	GCNGC	8 cut(s)	211, 247, 265, 1052, 2311, 2713, 2729, 3734
BlnI	CCTAGG	1 cut(s)	2426
BlsI	GCNGC	8 cut(s)	212, 248, 266, 1053, 2312, 2714, 2730, 3735
Bme1390I	CCNGG	8 cut(s)	579, 677, 1194, 1455, 1545, 2119, 2175, 2259
Bme18I	GGWCC	5 cut(s)	941, 1432, 2087, 3161, 3878
BmeT110I	CYCGRG	2 cut(s)	1490, 2102
BmgT120I	GGNCC	9 cut(s)	91, 941, 1432, 2087, 2590, 2858, 3161, 3735, 3878
BmiI	GGNNCC	7 cut(s)	588, 681, 730, 1804, 2253, 2859, 3737
BmrFI	CCNGG	8 cut(s)	579, 677, 1194, 1455, 1545, 2119, 2175, 2259
BmsI	GCATC	7 cut(s)	2005, 3210, 3257, 3300, 3304, 3448, 3721
BoxI	GACNNNNGTC	1 cut(s)	3876
BpiI	GAAGAC	1 cut(s)	2042
BplI	GAGNNNNNCTC	2 cut(s)	242, 274
BpmI	CTGGAG	1 cut(s)	2955
Bpu10I	CCTNAGC	4 cut(s)	242, 2708, 3081, 3333
Bpu14I	TTCGAA	1 cut(s)	336
BpuEI	CTTGAG	6 cut(s)	521, 646, 1249, 2902, 3468, 3483
BsaAI	YACGTR	2 cut(s)	2345, 3614
BsaHI	GRCGYC	1 cut(s)	3477
BsaI	GGTCTC	1 cut(s)	2099
BsaJI	CCNNGG	7 cut(s)	577, 1193, 1695, 1993, 2426, 2580, 3157
BsaWI	WCCGGW	2 cut(s)	736, 1255
BsaXI	ACNNNNNCTCC	4 cut(s)	2305, 2335, 2389, 2419
Bse1I	ACTGG	7 cut(s)	891, 1873, 2320, 2404, 2625, 3880, 3992
Bse3DI	GCAATG	3 cut(s)	104, 2208, 3728
BseAI	TCCGGA	1 cut(s)	1255
BseBI	CCWGG	8 cut(s)	579, 677, 1194, 1455, 1545, 2119, 2175, 2259
BseDI	CCNNGG	7 cut(s)	577, 1193, 1695, 1993, 2426, 2580, 3157
BseGI	GGATG	9 cut(s)	60, 568, 573, 2102, 2654, 3060, 3319, 3343, 3814
BseMI	GCAATG	3 cut(s)	104, 2208, 3728
BseNI	ACTGG	7 cut(s)	891, 1873, 2320, 2404, 2625, 3880, 3992
BseRI	GAGGAG	5 cut(s)	1867, 2853, 3132, 3832, 3835
BseSI	GKGCMC	1 cut(s)	3613
BseXI	GCAGC	6 cut(s)	222, 258, 276, 1038, 2322, 2740
BseYI	CCCAGC	1 cut(s)	2798
Bsh1236I	CGCG	2 cut(s)	3521, 3523
BshFI	GGCC	9 cut(s)	92, 323, 917, 998, 1251, 2592, 2715, 2860, 3736
BshNI	GGYRCC	2 cut(s)	679, 2251
BsiHKAI	GWGCWC	2 cut(s)	2456, 3613
BsiHKCI	CYCGRG	2 cut(s)	1490, 2102
BsiSI	CCGG	3 cut(s)	737, 806, 1256
BslFI	GGGAC	3 cut(s)	1611, 2085, 3838
BsmAI	GTCTC	8 cut(s)	290, 992, 1035, 1117, 2099, 2770, 3484, 3569
BsmBI	CGTCTC	2 cut(s)	3484, 3569
BsmFI	GGGAC	3 cut(s)	1611, 2085, 3838
BsmI	GAATGC	1 cut(s)	2368
BsnI	GGCC	9 cut(s)	92, 323, 917, 998, 1251, 2592, 2715, 2860, 3736
Bso31I	GGTCTC	1 cut(s)	2099
BsoBI	CYCGRG	2 cut(s)	1490, 2102
Bsp119I	TTCGAA	1 cut(s)	336
Bsp1286I	GDGCHC	5 cut(s)	509, 2456, 3613, 3909, 3939
Bsp13I	TCCGGA	1 cut(s)	1255
Bsp19I	CCATGG	1 cut(s)	2580
BspACI	CCGC	6 cut(s)	66, 2291, 2375, 2712, 2726, 3733
BspANI	GGCC	9 cut(s)	92, 323, 917, 998, 1251, 2592, 2715, 2860, 3736
BspEI	TCCGGA	1 cut(s)	1255
BspFNI	CGCG	2 cut(s)	3521, 3523
BspLI	GGNNCC	7 cut(s)	588, 681, 730, 1804, 2253, 2859, 3737
BspMAI	CTGCAG	1 cut(s)	1056
BspPI	GGATC	4 cut(s)	763, 1171, 1879, 2064
BspT104I	TTCGAA	1 cut(s)	336
BspT107I	GGYRCC	2 cut(s)	679, 2251
BspTNI	GGTCTC	1 cut(s)	2099
BsrBI	CCGCTC	1 cut(s)	2712
BsrDI	GCAATG	3 cut(s)	104, 2208, 3728
BsrI	ACTGG	7 cut(s)	891, 1873, 2320, 2404, 2625, 3880, 3992
BssECI	CCNNGG	7 cut(s)	577, 1193, 1695, 1993, 2426, 2580, 3157
BssNI	GRCGYC	1 cut(s)	3477
BssT1I	CCWWGG	4 cut(s)	1993, 2426, 2580, 3157
Bst2UI	CCWGG	8 cut(s)	579, 677, 1194, 1455, 1545, 2119, 2175, 2259
Bst4CI	ACNGT	7 cut(s)	49, 986, 2156, 3165, 3948, 4012, 4027
Bst6I	CTCTTC	3 cut(s)	293, 1120, 3567
BstACI	GRCGYC	1 cut(s)	3477
BstBAI	YACGTR	2 cut(s)	2345, 3614
BstBI	TTCGAA	1 cut(s)	336
BstC8I	GCNNGC	6 cut(s)	372, 439, 2199, 2733, 3246, 3837
BstDSI	CCRYGG	2 cut(s)	1695, 2580
BstEII	GGTNACC	1 cut(s)	2222
BstF5I	GGATG	9 cut(s)	60, 568, 573, 2102, 2654, 3060, 3319, 3343, 3814
BstFNI	CGCG	2 cut(s)	3521, 3523
BstHHI	GCGC	2 cut(s)	3523, 3835
BstMAI	GTCTC	8 cut(s)	290, 992, 1035, 1117, 2099, 2770, 3484, 3569
BstMWI	GCNNNNNNNGC	4 cut(s)	2288, 2372, 3281, 3467
BstNI	CCWGG	8 cut(s)	579, 677, 1194, 1455, 1545, 2119, 2175, 2259
BstNSI	RCATGY	2 cut(s)	2985, 3119
BstPAI	GACNNNNGTC	1 cut(s)	3876
BstPI	GGTNACC	1 cut(s)	2222
BstSCI	CCNGG	8 cut(s)	577, 675, 1192, 1453, 1543, 2117, 2173, 2257
BstSFI	CTRYAG	2 cut(s)	1052, 3043
BstSLI	GKGCMC	1 cut(s)	3613
BstUI	CGCG	2 cut(s)	3521, 3523
BstV1I	GCAGC	6 cut(s)	222, 258, 276, 1038, 2322, 2740
BstV2I	GAAGAC	1 cut(s)	2042
BstX2I	RGATCY	4 cut(s)	755, 844, 1063, 1871
BstXI	CCANNNNNNTGG	1 cut(s)	2587
BstYI	RGATCY	4 cut(s)	755, 844, 1063, 1871
BsuI	GTATCC	1 cut(s)	2869
BsuRI	GGCC	9 cut(s)	92, 323, 917, 998, 1251, 2592, 2715, 2860, 3736
BtgI	CCRYGG	2 cut(s)	1695, 2580
BtsCI	GGATG	9 cut(s)	60, 568, 573, 2102, 2654, 3060, 3319, 3343, 3814
BtsI	GCAGTG	1 cut(s)	1762
BtsIMutI	CAGTG	8 cut(s)	45, 884, 1762, 2313, 2397, 2968, 3873, 3985
Cac8I	GCNNGC	6 cut(s)	372, 439, 2199, 2733, 3246, 3837
CaiI	CAGNNNCTG	2 cut(s)	81, 3550
CfoI	GCGC	2 cut(s)	3523, 3835
Cfr13I	GGNCC	9 cut(s)	91, 941, 1432, 2087, 2590, 2858, 3161, 3735, 3878
CseI	GACGC	1 cut(s)	3586
CsiI	ACCWGGT	3 cut(s)	1543, 2173, 2257
CspCI	CAANNNNNGTGG	2 cut(s)	1213, 1248
DraIII	CACNNNGTG	1 cut(s)	2348
DrdI	GACNNNNNNGTC	1 cut(s)	691
DseDI	GACNNNNNNGTC	1 cut(s)	691
EaeI	YGGCCR	3 cut(s)	321, 996, 2713
Eam1104I	CTCTTC	3 cut(s)	293, 1120, 3567
EarI	CTCTTC	3 cut(s)	293, 1120, 3567
Ecl136II	GAGCTC	1 cut(s)	2454
Eco130I	CCWWGG	4 cut(s)	1993, 2426, 2580, 3157
Eco24I	GRGCYC	4 cut(s)	509, 2456, 3909, 3939
Eco31I	GGTCTC	1 cut(s)	2099
Eco47I	GGWCC	5 cut(s)	941, 1432, 2087, 3161, 3878
Eco53kI	GAGCTC	1 cut(s)	2454
Eco57I	CTGAAG	7 cut(s)	290, 1184, 1331, 1382, 2144, 3761, 3866
Eco72I	CACGTG	1 cut(s)	3614
Eco88I	CYCGRG	2 cut(s)	1490, 2102
Eco91I	GGTNACC	1 cut(s)	2222
EcoICRI	GAGCTC	1 cut(s)	2454
EcoO109I	RGGNCCY	1 cut(s)	2858
EcoO65I	GGTNACC	1 cut(s)	2222
EcoRI	GAATTC	2 cut(s)	169, 2929
EcoRII	CCWGG	8 cut(s)	577, 675, 1192, 1453, 1543, 2117, 2173, 2257
EcoT14I	CCWWGG	4 cut(s)	1993, 2426, 2580, 3157
EcoT38I	GRGCYC	4 cut(s)	509, 2456, 3909, 3939
ErhI	CCWWGG	4 cut(s)	1993, 2426, 2580, 3157
Esp3I	CGTCTC	2 cut(s)	3484, 3569
FalI	AAGNNNNNCTT	6 cut(s)	3264, 3296, 3363, 3395, 3654, 3686
FaqI	GGGAC	3 cut(s)	1611, 2085, 3838
FauI	CCCGC	1 cut(s)	73
Fnu4HI	GCNGC	8 cut(s)	211, 247, 265, 1052, 2311, 2713, 2729, 3734
FokI	GGATG	9 cut(s)	47, 560, 575, 2109, 2641, 3047, 3326, 3350, 3821
FriOI	GRGCYC	4 cut(s)	509, 2456, 3909, 3939
Fsp4HI	GCNGC	8 cut(s)	211, 247, 265, 1052, 2311, 2713, 2729, 3734
GlaI	GCGC	2 cut(s)	3522, 3834
GluI	GCNGC	8 cut(s)	211, 247, 265, 1052, 2311, 2713, 2729, 3734
GsaI	CCCAGC	1 cut(s)	2802
GsuI	CTGGAG	1 cut(s)	2955
HaeIII	GGCC	9 cut(s)	92, 323, 917, 998, 1251, 2592, 2715, 2860, 3736
HapII	CCGG	3 cut(s)	737, 806, 1256
HgaI	GACGC	1 cut(s)	3586
HhaI	GCGC	2 cut(s)	3523, 3835
Hin1I	GRCGYC	1 cut(s)	3477
Hin6I	GCGC	2 cut(s)	3521, 3833
HinP1I	GCGC	2 cut(s)	3521, 3833
HincII	GTYRAC	1 cut(s)	700
HindII	GTYRAC	1 cut(s)	700
HindIII	AAGCTT	6 cut(s)	155, 368, 977, 1975, 2126, 3273
HpaI	GTTAAC	1 cut(s)	700
HpaII	CCGG	3 cut(s)	737, 806, 1256
HphI	GGTGA	8 cut(s)	1678, 1691, 2177, 2225, 2331, 2412, 2430, 3356
Hpy166II	GTNNAC	7 cut(s)	73, 700, 2303, 3121, 3611, 3763, 3794
Hpy8I	GTNNAC	7 cut(s)	73, 700, 2303, 3121, 3611, 3763, 3794
Hpy99I	CGWCG	2 cut(s)	3005, 3792
HpyCH4III	ACNGT	7 cut(s)	49, 986, 2156, 3165, 3948, 4012, 4027
HpyCH4IV	ACGT	5 cut(s)	2344, 2357, 3477, 3613, 3790
HpyF10VI	GCNNNNNNNGC	4 cut(s)	2288, 2372, 3281, 3467
HpySE526I	ACGT	5 cut(s)	2344, 2357, 3477, 3613, 3790
Hsp92I	GRCGYC	1 cut(s)	3477
HspAI	GCGC	2 cut(s)	3521, 3833
Kpn2I	TCCGGA	1 cut(s)	1255
KpnI	GGTACC	2 cut(s)	683, 2255
KspAI	GTTAAC	1 cut(s)	700
LmnI	GCTCC	8 cut(s)	114, 653, 869, 1480, 1540, 2371, 2509, 3647
Lsp1109I	GCAGC	6 cut(s)	222, 258, 276, 1038, 2322, 2740
LweI	GCATC	7 cut(s)	2005, 3210, 3257, 3300, 3304, 3448, 3721
MabI	ACCWGGT	3 cut(s)	1543, 2173, 2257
MaeII	ACGT	5 cut(s)	2344, 2357, 3477, 3613, 3790
MbiI	CCGCTC	1 cut(s)	2712
MfeI	CAATTG	6 cut(s)	902, 1091, 1468, 1668, 1966, 3651
MflI	RGATCY	4 cut(s)	755, 844, 1063, 1871
MhlI	GDGCHC	5 cut(s)	509, 2456, 3613, 3909, 3939
MlsI	TGGCCA	1 cut(s)	998
MluNI	TGGCCA	1 cut(s)	998
MlyI	GAGTC	9 cut(s)	260, 637, 803, 807, 1268, 1816, 2690, 3490, 3860
MmeI	TCCRAC	5 cut(s)	211, 316, 2097, 2393, 3846
Mox20I	TGGCCA	1 cut(s)	998
MroI	TCCGGA	1 cut(s)	1255
MroXI	GAANNNNTTC	3 cut(s)	1270, 1388, 3546
MscI	TGGCCA	1 cut(s)	998
MslI	CAYNNNNRTG	1 cut(s)	2585
Msp20I	TGGCCA	1 cut(s)	998
MspA1I	CMGCKG	1 cut(s)	3085
MspI	CCGG	3 cut(s)	737, 806, 1256
MspR9I	CCNGG	8 cut(s)	579, 677, 1194, 1455, 1545, 2119, 2175, 2259
MunI	CAATTG	6 cut(s)	902, 1091, 1468, 1668, 1966, 3651
Mva1269I	GAATGC	1 cut(s)	2368
MvaI	CCWGG	8 cut(s)	579, 677, 1194, 1455, 1545, 2119, 2175, 2259
MvnI	CGCG	2 cut(s)	3521, 3523
MwoI	GCNNNNNNNGC	4 cut(s)	2288, 2372, 3281, 3467
NcoI	CCATGG	1 cut(s)	2580
NlaIV	GGNNCC	7 cut(s)	588, 681, 730, 1804, 2253, 2859, 3737
NmuCI	GTSAC	5 cut(s)	160, 705, 882, 1679, 3006
NspI	RCATGY	2 cut(s)	2985, 3119
NspV	TTCGAA	1 cut(s)	336
PaeR7I	CTCGAG	1 cut(s)	1490
PciI	ACATGT	1 cut(s)	2981
PctI	GAATGC	1 cut(s)	2368
PdmI	GAANNNNTTC	3 cut(s)	1270, 1388, 3546
PfeI	GAWTC	9 cut(s)	146, 764, 1495, 1567, 1591, 2791, 3547, 3860, 3980
PflMI	CCANNNNNTGG	1 cut(s)	960
PkrI	GCNGC	8 cut(s)	212, 248, 266, 1053, 2312, 2714, 2730, 3735
PleI	GAGTC	9 cut(s)	259, 636, 803, 806, 1267, 1816, 2689, 3490, 3859
PmaCI	CACGTG	1 cut(s)	3614
PmlI	CACGTG	1 cut(s)	3614
PpsI	GAGTC	9 cut(s)	259, 636, 803, 806, 1267, 1816, 2689, 3490, 3859
Ppu21I	YACGTR	2 cut(s)	2345, 3614
PscI	ACATGT	1 cut(s)	2981
PshAI	GACNNNNGTC	1 cut(s)	3876
PshBI	ATTAAT	2 cut(s)	948, 2001
Psp124BI	GAGCTC	1 cut(s)	2456
Psp6I	CCWGG	8 cut(s)	577, 675, 1192, 1453, 1543, 2117, 2173, 2257
PspCI	CACGTG	1 cut(s)	3614
PspEI	GGTNACC	1 cut(s)	2222
PspFI	CCCAGC	1 cut(s)	2798
PspGI	CCWGG	8 cut(s)	577, 675, 1192, 1453, 1543, 2117, 2173, 2257
PspN4I	GGNNCC	7 cut(s)	588, 681, 730, 1804, 2253, 2859, 3737
PspPI	GGNCC	9 cut(s)	91, 941, 1432, 2087, 2590, 2858, 3161, 3735, 3878
PstI	CTGCAG	1 cut(s)	1056
PstNI	CAGNNNCTG	2 cut(s)	81, 3550
PsuI	RGATCY	4 cut(s)	755, 844, 1063, 1871
PvuII	CAGCTG	1 cut(s)	3085
RseI	CAYNNNNRTG	1 cut(s)	2585
SacI	GAGCTC	1 cut(s)	2456
SatI	GCNGC	8 cut(s)	211, 247, 265, 1052, 2311, 2713, 2729, 3734
Sau96I	GGNCC	9 cut(s)	91, 941, 1432, 2087, 2590, 2858, 3161, 3735, 3878
SchI	GAGTC	9 cut(s)	260, 637, 803, 807, 1268, 1816, 2690, 3490, 3860
ScrFI	CCNGG	8 cut(s)	579, 677, 1194, 1455, 1545, 2119, 2175, 2259
SduI	GDGCHC	5 cut(s)	509, 2456, 3613, 3909, 3939
SexAI	ACCWGGT	3 cut(s)	1543, 2173, 2257
SfaNI	GCATC	7 cut(s)	2005, 3210, 3257, 3300, 3304, 3448, 3721
SfcI	CTRYAG	2 cut(s)	1052, 3043
Sfr274I	CTCGAG	1 cut(s)	1490
SfuI	TTCGAA	1 cut(s)	336
SinI	GGWCC	5 cut(s)	941, 1432, 2087, 3161, 3878
SlaI	CTCGAG	1 cut(s)	1490
SmiMI	CAYNNNNRTG	1 cut(s)	2585
SmlI	CTYRAG	7 cut(s)	500, 625, 1228, 1490, 2917, 3483, 3498
SmoI	CTYRAG	7 cut(s)	500, 625, 1228, 1490, 2917, 3483, 3498
SsiI	CCGC	6 cut(s)	66, 2291, 2375, 2712, 2726, 3733
SspI	AATATT	1 cut(s)	558
SstI	GAGCTC	1 cut(s)	2456
StyD4I	CCNGG	8 cut(s)	577, 675, 1192, 1453, 1543, 2117, 2173, 2257
StyI	CCWWGG	4 cut(s)	1993, 2426, 2580, 3157
TaaI	ACNGT	7 cut(s)	49, 986, 2156, 3165, 3948, 4012, 4027
TaiI	ACGT	5 cut(s)	2347, 2360, 3480, 3616, 3793
TatI	WGTACW	1 cut(s)	3213
TauI	GCSGC	2 cut(s)	2715, 3736
TfiI	GAWTC	9 cut(s)	146, 764, 1495, 1567, 1591, 2791, 3547, 3860, 3980
TscAI	CASTG	8 cut(s)	52, 891, 1762, 2320, 2404, 2968, 3880, 3992
TseFI	GTSAC	5 cut(s)	160, 705, 882, 1679, 3006
TseI	GCWGC	6 cut(s)	210, 246, 264, 1051, 2310, 2728
Tsp45I	GTSAC	5 cut(s)	160, 705, 882, 1679, 3006
TspDTI	ATGAA	6 cut(s)	1475, 2229, 2514, 3126, 3458, 3941
TspGWI	ACGGA	2 cut(s)	666, 2583
TspRI	CASTG	8 cut(s)	52, 891, 1762, 2320, 2404, 2968, 3880, 3992
Van91I	CCANNNNNTGG	1 cut(s)	960
VneI	GTGCAC	1 cut(s)	3609
VpaK11BI	GGWCC	5 cut(s)	941, 1432, 2087, 3161, 3878
VspI	ATTAAT	2 cut(s)	948, 2001
XbaI	TCTAGA	3 cut(s)	470, 1528, 3769
XceI	RCATGY	2 cut(s)	2985, 3119
XhoI	CTCGAG	1 cut(s)	1490
XmaJI	CCTAGG	1 cut(s)	2426
XmnI	GAANNNNTTC	3 cut(s)	1270, 1388, 3546
ZraI	GACGTC	1 cut(s)	3478

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.