Rorug03G0251900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0011470
Physical Location & Seq
Reverse (-)
23808592 .. 23811418
2827 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0251900.1

Sequence Viewer

Length: 1140 bp
ATGGCTGAGTACCCCTACTCATCAAATTTCTTCACAGGTCTCTTCAAATTCAACCCTCTTCTTCATCATCATCACCAACATGAATACCCTTTGATTTCAAACCCTCCTCCTCCTCCGCAACCACAACCACAACCTCAACCTCAACCTCCCTATGCTCACAACTTCTTCAATATCAACTACAATCAGAACCACAACCAGTACTCTTCTTTTTACTCCAGCCAAACCACTCATCATCAACCCCCTTCTCCACCTCTCAGAGAAGCCCTCCCACTCCTTAACCGCTTAAGCCCAACAAAGCATCATGAAGACGAAGAACAAGAGCATGATCACCATCATGAGTACTACTCCTGTAGTACTGATCAAATGGATGTAGATGACAAGATCAGCAAACCGTACAACAAAGAAGACCATGGGGAAGATGATGAGGCTAGTGTGACTGTTGCACTGCACATAGGACTCCCGAGCCCTAGTGCAGCTGAAATGGCATCTGTGCTTTCTGCAGCTTCTTCTTCCTCAGAAATCAGCACAACTCATGACAAAGATCATGTTATTGGAGATCATGGTGATGATTCAACTGGGTACCCCATGAACAGATCATCGCTCAACAAGGGTCAGTATTGGATCCCAACTCCGTCACAGATTCTCATTGGTCCTACTCAATTCTCTTGCCCTGTCTGCTTCAAGACCTTCAACAGATACAATAACATGCAGATGCATATGTGGGGGCATGGATCTCAATACAGAAAAGGGCCCGAGTCTCTAAGAGGAACTCAACCTACAGGCATGCTTAGGCTTCCTTGCTATTGTTGCACACCAGGTTGCAGGAACAACATCGATCACCCGAGAGCGAAGCCCCTGAAAGACTTTAGAACCCTCCAAACACATTACAAGAGGAAGCATGGAATCAAGCCTTTCATGTGCAGAAAATGTGGCAAGGCATTTGCAGTGAGAGGAGATTGGAGAACCCACGAGAAGAATTGTGGCAAGCTTTGGTATTGCATTTGTGGCTCTGATTTTAAGCACAAGAGGTCTCTCAAAGATCATATCAAGGCTTTTGGGAATGGGCATGCAGCTTATGGAATTGATGGATTTGAAGAAGATGATGAGCCTGCATCTGAAGTTGAGCAAGATAATGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00670 ko00970 map00670 map00970
Pfam Domains
Protein Families
No domains found.

Protein Analysis

379

Amino Acids

43.29

Weight (kDa)

6.48

Isoelectric Point (pI)

52.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C2H2_STOP2_3rd PF23115 267 - 301 1.3e-18 STOP2-like, C2H2-type zinc finger
C2CH-3rd_BIRD-IDD PF22995 304 - 328 7.7e-12 BIRD-IDD transcription factor, third C2HC zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011470)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G17460 AT5G17460 AT5G17460 AT5G17460 AT5G17460
fragaria_vesca FvH4_1g30050
malus_domestica MD09G1260700.v1.1 MD09G1260800.v1.1
prunus_persica Prupe.3G152500_v2.0.a1
pyrus_communis pycom09g17520
rosa_chinensis RchiOBHm_Chr3g0492091
rosa_laevigata RLG00000022794
rosa_multiflora Rmu_sc0021983.1_g000002
rosa_roxburghii Rroxscaffold_6G00392540
rosa_rugosa Rorug03G0251900
rosa_samantha Rh3AG302000 Rh3BG337900 Rh3CG335400 Rh3DG337500
rosa_wichuraiana Rw3G026660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 579
AccB1I GGYRCC 1 cut(s) 579
AciI CCGC 2 cut(s) 116, 280
AclWI GGATC 3 cut(s) 616, 629, 739
AcsI RAATTY 2 cut(s) 25, 47
AcuI CTGAAG 1 cut(s) 1137
AfaI GTAC 6 cut(s) 11, 200, 341, 355, 395, 581
AflII CTTAAG 1 cut(s) 283
AgsI TTSAA 8 cut(s) 46, 52, 99, 169, 573, 682, 691, 1094
AjnI CCWGG 1 cut(s) 814
AluBI AGCT 4 cut(s) 476, 503, 988, 1073
AluI AGCT 4 cut(s) 476, 503, 988, 1073
Alw26I GTCTC 3 cut(s) 44, 762, 1035
AlwI GGATC 3 cut(s) 616, 629, 739
Ama87I CYCGRG 3 cut(s) 460, 752, 841
AoxI GGCC 1 cut(s) 749
ApaI GGGCCC 1 cut(s) 753
ApeKI GCWGC 3 cut(s) 473, 500, 1070
ApoI RAATTY 2 cut(s) 25, 47
Asp718I GGTACC 1 cut(s) 579
AspS9I GGNCC 3 cut(s) 650, 749, 750
AsuHPI GGTGA 4 cut(s) 65, 320, 575, 830
AvaI CYCGRG 3 cut(s) 460, 752, 841
AvaII GGWCC 1 cut(s) 650
BaeGI GKGCMC 1 cut(s) 753
BamHI GGATCC 1 cut(s) 621
BanI GGYRCC 1 cut(s) 579
BanII GRGCYC 2 cut(s) 467, 753
BauI CACGAG 1 cut(s) 968
BbsI GAAGAC 2 cut(s) 312, 411
BbvI GCAGC 3 cut(s) 485, 512, 1082
BccI CCATC 2 cut(s) 339, 1079
BciT130I CCWGG 1 cut(s) 816
BclI TGATCA 2 cut(s) 325, 358
BcoDI GTCTC 3 cut(s) 44, 762, 1035
BfaI CTAG 2 cut(s) 429, 468
BfmI CTRYAG 3 cut(s) 349, 498, 777
BfrI CTTAAG 1 cut(s) 283
BisI GCNGC 3 cut(s) 474, 501, 1071
BlsI GCNGC 3 cut(s) 475, 502, 1072
BmcAI AGTACT 3 cut(s) 200, 341, 355
Bme1390I CCNGG 1 cut(s) 816
Bme18I GGWCC 1 cut(s) 650
BmeT110I CYCGRG 3 cut(s) 460, 752, 841
BmgT120I GGNCC 3 cut(s) 650, 749, 750
BmiI GGNNCC 3 cut(s) 581, 623, 751
BmrFI CCNGG 1 cut(s) 816
BmrI ACTGGG 1 cut(s) 585
BmsI GCATC 4 cut(s) 307, 494, 702, 1121
BmuI ACTGGG 1 cut(s) 585
BpiI GAAGAC 2 cut(s) 312, 411
BplI GAGNNNNNCTC 4 cut(s) 249, 281, 329, 361
BpmI CTGGAG 1 cut(s) 199
Bpu10I CCTNAGC 1 cut(s) 788
Bsa29I ATCGAT 1 cut(s) 834
BsaBI GATNNNNATC 1 cut(s) 330
BsaI GGTCTC 2 cut(s) 44, 1035
BsaJI CCNNGG 1 cut(s) 409
Bse1I ACTGG 2 cut(s) 196, 580
Bse8I GATNNNNATC 1 cut(s) 330
BseBI CCWGG 1 cut(s) 816
BseCI ATCGAT 1 cut(s) 834
BseDI CCNNGG 1 cut(s) 409
BseGI GGATG 1 cut(s) 373
BseJI GATNNNNATC 1 cut(s) 330
BseMII CTCAG 2 cut(s) 268, 528
BseNI ACTGG 2 cut(s) 196, 580
BseRI GAGGAG 4 cut(s) 96, 99, 102, 966
BseSI GKGCMC 1 cut(s) 753
BseXI GCAGC 3 cut(s) 485, 512, 1082
BsgI GTGCAG 3 cut(s) 431, 492, 940
BshFI GGCC 1 cut(s) 751
BshNI GGYRCC 1 cut(s) 579
BshVI ATCGAT 1 cut(s) 834
BsiHKCI CYCGRG 3 cut(s) 460, 752, 841
BsmAI GTCTC 3 cut(s) 44, 762, 1035
BsnI GGCC 1 cut(s) 751
Bso31I GGTCTC 2 cut(s) 44, 1035
BsoBI CYCGRG 3 cut(s) 460, 752, 841
Bsp120I GGGCCC 1 cut(s) 749
Bsp1286I GDGCHC 2 cut(s) 467, 753
Bsp19I CCATGG 1 cut(s) 409
BspACI CCGC 2 cut(s) 116, 280
BspANI GGCC 1 cut(s) 751
BspCNI CTCAG 2 cut(s) 267, 527
BspDI ATCGAT 1 cut(s) 834
BspHI TCATGA 3 cut(s) 301, 334, 532
BspLI GGNNCC 3 cut(s) 581, 623, 751
BspMAI CTGCAG 1 cut(s) 502
BspPI GGATC 3 cut(s) 616, 629, 739
BspT107I GGYRCC 1 cut(s) 579
BspTI CTTAAG 1 cut(s) 283
BspTNI GGTCTC 2 cut(s) 44, 1035
BsrI ACTGG 2 cut(s) 196, 580
BssECI CCNNGG 1 cut(s) 409
BssSI CACGAG 1 cut(s) 968
BssT1I CCWWGG 1 cut(s) 409
Bst2BI CACGAG 1 cut(s) 968
Bst2UI CCWGG 1 cut(s) 816
Bst4CI ACNGT 2 cut(s) 393, 439
Bst6I CTCTTC 3 cut(s) 47, 63, 208
BstAFI CTTAAG 1 cut(s) 283
BstC8I GCNNGC 4 cut(s) 785, 986, 1068, 1110
BstDEI CTNAG 5 cut(s) 6, 254, 514, 761, 788
BstDSI CCRYGG 1 cut(s) 409
BstF5I GGATG 1 cut(s) 373
BstMAI GTCTC 3 cut(s) 44, 762, 1035
BstMWI GCNNNNNNNGC 4 cut(s) 482, 675, 807, 1005
BstNI CCWGG 1 cut(s) 816
BstNSI RCATGY 3 cut(s) 709, 787, 1070
BstSCI CCNGG 1 cut(s) 814
BstSFI CTRYAG 3 cut(s) 349, 498, 777
BstSLI GKGCMC 1 cut(s) 753
BstV1I GCAGC 3 cut(s) 485, 512, 1082
BstV2I GAAGAC 2 cut(s) 312, 411
BstX2I RGATCY 2 cut(s) 621, 731
BstYI RGATCY 2 cut(s) 621, 731
Bsu15I ATCGAT 1 cut(s) 834
BsuRI GGCC 1 cut(s) 751
BsuTUI ATCGAT 1 cut(s) 834
BtgI CCRYGG 1 cut(s) 409
BtgZI GCGATG 1 cut(s) 582
BtsCI GGATG 1 cut(s) 373
BtsI GCAGTG 2 cut(s) 443, 951
BtsIMutI CAGTG 2 cut(s) 443, 951
Cac8I GCNNGC 4 cut(s) 785, 986, 1068, 1110
CciI TCATGA 3 cut(s) 301, 334, 532
Cfr13I GGNCC 3 cut(s) 650, 749, 750
ClaI ATCGAT 1 cut(s) 834
CsiI ACCWGGT 1 cut(s) 814
Csp6I GTAC 6 cut(s) 10, 199, 340, 354, 394, 580
CviQI GTAC 6 cut(s) 10, 199, 340, 354, 394, 580
DdeI CTNAG 5 cut(s) 6, 254, 514, 761, 788
Eam1104I CTCTTC 3 cut(s) 47, 63, 208
EarI CTCTTC 3 cut(s) 47, 63, 208
Eco130I CCWWGG 1 cut(s) 409
Eco24I GRGCYC 2 cut(s) 467, 753
Eco31I GGTCTC 2 cut(s) 44, 1035
Eco47I GGWCC 1 cut(s) 650
Eco57I CTGAAG 1 cut(s) 1137
Eco88I CYCGRG 3 cut(s) 460, 752, 841
EcoO109I RGGNCCY 1 cut(s) 749
EcoRII CCWGG 1 cut(s) 814
EcoT14I CCWWGG 1 cut(s) 409
EcoT22I ATGCAT 1 cut(s) 717
EcoT38I GRGCYC 2 cut(s) 467, 753
ErhI CCWWGG 1 cut(s) 409
FauNDI CATATG 1 cut(s) 717
FbaI TGATCA 2 cut(s) 325, 358
Fnu4HI GCNGC 3 cut(s) 474, 501, 1071
FokI GGATG 1 cut(s) 380
FriOI GRGCYC 2 cut(s) 467, 753
Fsp4HI GCNGC 3 cut(s) 474, 501, 1071
FspBI CTAG 2 cut(s) 429, 468
GluI GCNGC 3 cut(s) 474, 501, 1071
GsuI CTGGAG 1 cut(s) 199
HaeIII GGCC 1 cut(s) 751
HindIII AAGCTT 1 cut(s) 986
HinfI GANTC 5 cut(s) 456, 569, 640, 755, 903
HphI GGTGA 4 cut(s) 65, 320, 575, 830
Hpy188I TCNGA 5 cut(s) 186, 257, 517, 1012, 1117
Hpy188III TCNNGA 5 cut(s) 302, 335, 460, 533, 682
HpyAV CCTTC 2 cut(s) 252, 697
HpyCH4III ACNGT 2 cut(s) 393, 439
HpyF10VI GCNNNNNNNGC 4 cut(s) 482, 675, 807, 1005
HpyF3I CTNAG 5 cut(s) 6, 254, 514, 761, 788
KpnI GGTACC 1 cut(s) 583
Ksp22I TGATCA 2 cut(s) 325, 358
Lsp1109I GCAGC 3 cut(s) 485, 512, 1082
LweI GCATC 4 cut(s) 307, 494, 702, 1121
MabI ACCWGGT 1 cut(s) 814
MaeI CTAG 2 cut(s) 429, 468
MaeIII GTNAC 2 cut(s) 433, 633
MflI RGATCY 2 cut(s) 621, 731
MhlI GDGCHC 2 cut(s) 467, 753
MluCI AATT 5 cut(s) 25, 47, 659, 976, 1080
MlyI GAGTC 2 cut(s) 450, 764
Mph1103I ATGCAT 1 cut(s) 717
MseI TTAA 4 cut(s) 276, 284, 1017, 1138
MslI CAYNNNNRTG 4 cut(s) 78, 333, 564, 710
MspA1I CMGCKG 1 cut(s) 476
MspCI CTTAAG 1 cut(s) 283
MspR9I CCNGG 1 cut(s) 816
MvaI CCWGG 1 cut(s) 816
MwoI GCNNNNNNNGC 4 cut(s) 482, 675, 807, 1005
NcoI CCATGG 1 cut(s) 409
NdeI CATATG 1 cut(s) 717
NlaIV GGNNCC 3 cut(s) 581, 623, 751
NmuCI GTSAC 2 cut(s) 433, 633
NsiI ATGCAT 1 cut(s) 717
NspI RCATGY 3 cut(s) 709, 787, 1070
PaeI GCATGC 2 cut(s) 787, 1070
PagI TCATGA 3 cut(s) 301, 334, 532
PfeI GAWTC 3 cut(s) 569, 640, 903
PkrI GCNGC 3 cut(s) 475, 502, 1072
PleI GAGTC 2 cut(s) 450, 763
PpsI GAGTC 2 cut(s) 450, 763
Psp6I CCWGG 1 cut(s) 814
PspGI CCWGG 1 cut(s) 814
PspN4I GGNNCC 3 cut(s) 581, 623, 751
PspOMI GGGCCC 1 cut(s) 749
PspPI GGNCC 3 cut(s) 650, 749, 750
PsrI GAACNNNNNNTAC 2 cut(s) 760, 792
PstI CTGCAG 1 cut(s) 502
PsuI RGATCY 2 cut(s) 621, 731
PvuII CAGCTG 1 cut(s) 476
RsaI GTAC 6 cut(s) 11, 200, 341, 355, 395, 581
RsaNI GTAC 6 cut(s) 10, 199, 340, 354, 394, 580
RseI CAYNNNNRTG 4 cut(s) 78, 333, 564, 710
SaqAI TTAA 4 cut(s) 276, 284, 1017, 1138
SatI GCNGC 3 cut(s) 474, 501, 1071
Sau96I GGNCC 3 cut(s) 650, 749, 750
ScaI AGTACT 3 cut(s) 200, 341, 355
SchI GAGTC 2 cut(s) 450, 764
ScrFI CCNGG 1 cut(s) 816
SduI GDGCHC 2 cut(s) 467, 753
SexAI ACCWGGT 1 cut(s) 814
SfaNI GCATC 4 cut(s) 307, 494, 702, 1121
SfcI CTRYAG 3 cut(s) 349, 498, 777
SinI GGWCC 1 cut(s) 650
SmiMI CAYNNNNRTG 4 cut(s) 78, 333, 564, 710
SmlI CTYRAG 1 cut(s) 283
SmoI CTYRAG 1 cut(s) 283
SphI GCATGC 2 cut(s) 787, 1070
Sse9I AATT 5 cut(s) 25, 47, 659, 976, 1080
SsiI CCGC 2 cut(s) 116, 280
SspMI CTAG 2 cut(s) 429, 468
StyD4I CCNGG 1 cut(s) 814
StyI CCWWGG 1 cut(s) 409
TaaI ACNGT 2 cut(s) 393, 439
TaqI TCGA 1 cut(s) 834
TasI AATT 5 cut(s) 25, 47, 659, 976, 1080
TatI WGTACW 3 cut(s) 198, 339, 353
TfiI GAWTC 3 cut(s) 569, 640, 903
Tru1I TTAA 4 cut(s) 276, 284, 1017, 1138
Tru9I TTAA 4 cut(s) 276, 284, 1017, 1138
TscAI CASTG 2 cut(s) 450, 951
TseFI GTSAC 2 cut(s) 433, 633
TseI GCWGC 3 cut(s) 473, 500, 1070
Tsp45I GTSAC 2 cut(s) 433, 633
TspDTI ATGAA 5 cut(s) 53, 96, 318, 602, 904
TspGWI ACGGA 1 cut(s) 621
TspRI CASTG 2 cut(s) 450, 951
Vha464I CTTAAG 1 cut(s) 283
VpaK11BI GGWCC 1 cut(s) 650
XapI RAATTY 2 cut(s) 25, 47
XceI RCATGY 3 cut(s) 709, 787, 1070
XspI CTAG 2 cut(s) 429, 468
ZrmI AGTACT 3 cut(s) 200, 341, 355
Zsp2I ATGCAT 1 cut(s) 717
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.