Rorug03G0286400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0007773
Physical Location & Seq
Reverse (-)
28964642 .. 28966217
1576 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0286400.1

Sequence Viewer

Length: 1092 bp
ATGGCAGCAGAGGAAACTGGTAAATGCTCTGAAGCTTATCCAATGAAAGGTGGAGATGGCCCCAGCAACTATGCCAAGAACTCCATCTACCAGAAAGGAGTAATTGATGCTGCGAAAGAACTTCTAAATAAGGCGATTGCAGAAAAGCTTGACATGGAAACATTTTCATCTGCCAACTCCTTTCACATTGCAGATTTGGGTTGCTCAGTTGGGCCTAATACATTTTTCGCAGTTGAAAACATACTTGAAGCTGTTCTATTCAAGTATCAAAGCCGAGGGCTGAATTGTCAAATCCCTGAATTTCAAGTCTTCTTTAATGATCATACCTCAAATGACTTCAACATGCTCTTCAATTCCCTCCCTCAGAATAGGCAATACTATGCTGCGGCTGTGCCTGGTTCTTTCTATGGTCGAATACTCCCCGATGCTTCCATCCACTTTTTTCACTCTTCTATTTCCCTTCAGTGGCTTTCTAGAGTACCAAAAGATGTAACAGACAGCAACTCCCCTGCTTGGAATAAAGGACGAATACATTACTTAGACTCCACAGATGAAGTAGTGAGGGCTTACGAAGCCCAATATGCCGAGGACATGGAGCACTTTCTGCATGCCAGGGCACAAGAGACAGTACATGGAGGATTGATGGTACTTGCCATTCCTGGTTACCCAGCTGATACACCTCCTTCTCATACTCTGGCAAATGTCATCTATCAAATTTTAGGATCTTGCCTCATTGACATGGCTAGGAAGGGAGTAGTCAGCGAGGAGAAAATAGATTCATTTAATGTGCCTATATACTATGTGGGTCCCCGAGAACTGGAAGCTGCTGTAGAGCGAAATGGATGTTTTAGCATAGAGATAATGGAACACTTACCTACTGTGATGGAACCCGGAACTATTTCAAAAAATAGCAAACTCTTTGCATCTCATATGAGAGCTATCATGGAAGGACTCTTCAAGCAGCATTTCAAAGAAGAAATCTTAGATGAGCTCTTCGACTTGTTTCACAAGAAAGTTGAAGAGCAGCACTCCGCATTTGAGTCAGCGAAGGGAGTGGACATCCTTATTGTTCTTAAACGCATGGCAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

363

Amino Acids

40.67

Weight (kDa)

5.38

Isoelectric Point (pI)

41.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 50 - 360 3.9e-106 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 386, 1032
AclWI GGATC 1 cut(s) 730
AcsI RAATTY 2 cut(s) 299, 714
AcuI CTGAAG 2 cut(s) 51, 446
AfaI GTAC 3 cut(s) 480, 630, 648
AfiI CCNNNNNNNGG 4 cut(s) 47, 465, 513, 817
AjnI CCWGG 3 cut(s) 394, 611, 658
AluBI AGCT 7 cut(s) 35, 148, 251, 671, 824, 938, 991
AluI AGCT 7 cut(s) 35, 148, 251, 671, 824, 938, 991
Alw21I GWGCWC 2 cut(s) 600, 993
Alw26I GTCTC 1 cut(s) 617
AlwI GGATC 1 cut(s) 730
Ama87I CYCGRG 1 cut(s) 810
AoxI GGCC 2 cut(s) 58, 212
ApeKI GCWGC 6 cut(s) 5, 110, 383, 824, 961, 1024
ApoI RAATTY 2 cut(s) 299, 714
Asp700I GAANNNNTTC 2 cut(s) 252, 898
AspS9I GGNCC 3 cut(s) 59, 212, 806
AsuC2I CCSGG 1 cut(s) 891
AvaI CYCGRG 1 cut(s) 810
AvaII GGWCC 1 cut(s) 806
BaeGI GKGCMC 1 cut(s) 619
BanII GRGCYC 1 cut(s) 993
BbsI GAAGAC 1 cut(s) 301
Bbv12I GWGCWC 2 cut(s) 600, 993
BbvI GCAGC 6 cut(s) 17, 97, 370, 811, 973, 1036
BccI CCATC 5 cut(s) 50, 92, 440, 637, 877
BciT130I CCWGG 3 cut(s) 396, 613, 660
BclI TGATCA 1 cut(s) 319
BcnI CCSGG 1 cut(s) 891
BcoDI GTCTC 1 cut(s) 617
BfaI CTAG 2 cut(s) 474, 744
BfmI CTRYAG 1 cut(s) 828
BisI GCNGC 7 cut(s) 6, 111, 384, 387, 825, 962, 1025
BlsI GCNGC 7 cut(s) 7, 112, 385, 388, 826, 963, 1026
Bme1390I CCNGG 4 cut(s) 396, 613, 660, 891
Bme18I GGWCC 1 cut(s) 806
BmeT110I CYCGRG 1 cut(s) 810
BmgT120I GGNCC 3 cut(s) 59, 212, 806
BmiI GGNNCC 4 cut(s) 61, 807, 808, 888
BmrFI CCNGG 4 cut(s) 396, 613, 660, 891
BmsI GCATC 3 cut(s) 97, 415, 932
BpiI GAAGAC 1 cut(s) 301
BplI GAGNNNNNCTC 2 cut(s) 1013, 1045
BpuMI CCSGG 1 cut(s) 891
BsaJI CCNNGG 3 cut(s) 274, 585, 612
BsaXI ACNNNNNCTCC 4 cut(s) 488, 518, 527, 557
Bsc4I CCNNNNNNNGG 4 cut(s) 47, 465, 513, 817
Bse1I ACTGG 2 cut(s) 22, 822
Bse3DI GCAATG 1 cut(s) 186
BseBI CCWGG 3 cut(s) 396, 613, 660
BseDI CCNNGG 3 cut(s) 274, 585, 612
BseGI GGATG 3 cut(s) 432, 848, 1059
BseLI CCNNNNNNNGG 4 cut(s) 47, 465, 513, 817
BseMI GCAATG 1 cut(s) 186
BseMII CTCAG 2 cut(s) 219, 377
BseNI ACTGG 2 cut(s) 22, 822
BseRI GAGGAG 1 cut(s) 779
BseSI GKGCMC 1 cut(s) 619
BseXI GCAGC 6 cut(s) 17, 97, 370, 811, 973, 1036
BseYI CCCAGC 2 cut(s) 62, 667
BshFI GGCC 2 cut(s) 60, 214
BsiHKAI GWGCWC 2 cut(s) 600, 993
BsiHKCI CYCGRG 1 cut(s) 810
BsiSI CCGG 1 cut(s) 891
BslFI GGGAC 1 cut(s) 792
BslI CCNNNNNNNGG 4 cut(s) 47, 465, 513, 817
BsmAI GTCTC 1 cut(s) 617
BsmFI GGGAC 1 cut(s) 792
BsnI GGCC 2 cut(s) 60, 214
BsoBI CYCGRG 1 cut(s) 810
Bsp1286I GDGCHC 3 cut(s) 600, 619, 993
Bsp143I GATC 2 cut(s) 319, 722
BspACI CCGC 2 cut(s) 386, 1032
BspANI GGCC 2 cut(s) 60, 214
BspCNI CTCAG 2 cut(s) 218, 376
BspLI GGNNCC 4 cut(s) 61, 807, 808, 888
BspPI GGATC 1 cut(s) 730
BspQI GCTCTTC 3 cut(s) 353, 998, 1014
BsrDI GCAATG 1 cut(s) 186
BsrI ACTGG 2 cut(s) 22, 822
BssECI CCNNGG 3 cut(s) 274, 585, 612
BssMI GATC 2 cut(s) 319, 722
Bst2UI CCWGG 3 cut(s) 396, 613, 660
Bst4CI ACNGT 2 cut(s) 628, 880
Bst6I CTCTTC 5 cut(s) 353, 454, 959, 998, 1014
BstAPI GCANNNNNTGC 1 cut(s) 604
BstC8I GCNNGC 1 cut(s) 609
BstDEI CTNAG 4 cut(s) 205, 363, 538, 982
BstEII GGTNACC 1 cut(s) 662
BstF5I GGATG 3 cut(s) 432, 848, 1059
BstKTI GATC 2 cut(s) 322, 725
BstMAI GTCTC 1 cut(s) 617
BstMBI GATC 2 cut(s) 319, 722
BstMWI GCNNNNNNNGC 3 cut(s) 572, 581, 604
BstNI CCWGG 3 cut(s) 396, 613, 660
BstNSI RCATGY 2 cut(s) 346, 611
BstPI GGTNACC 1 cut(s) 662
BstSCI CCNGG 4 cut(s) 394, 611, 658, 889
BstSFI CTRYAG 1 cut(s) 828
BstSLI GKGCMC 1 cut(s) 619
BstV1I GCAGC 6 cut(s) 17, 97, 370, 811, 973, 1036
BstV2I GAAGAC 1 cut(s) 301
BstX2I RGATCY 1 cut(s) 722
BstYI RGATCY 1 cut(s) 722
BsuRI GGCC 2 cut(s) 60, 214
BtsCI GGATG 3 cut(s) 432, 848, 1059
BtsIMutI CAGTG 1 cut(s) 470
Cac8I GCNNGC 1 cut(s) 609
Cfr13I GGNCC 3 cut(s) 59, 212, 806
Csp6I GTAC 3 cut(s) 479, 629, 647
CviAII CATG 8 cut(s) 154, 343, 592, 608, 632, 739, 943, 1081
CviQI GTAC 3 cut(s) 479, 629, 647
DdeI CTNAG 4 cut(s) 205, 363, 538, 982
DpnI GATC 2 cut(s) 321, 724
DpnII GATC 2 cut(s) 319, 722
Eam1104I CTCTTC 5 cut(s) 353, 454, 959, 998, 1014
EarI CTCTTC 5 cut(s) 353, 454, 959, 998, 1014
Ecl136II GAGCTC 1 cut(s) 991
Eco24I GRGCYC 1 cut(s) 993
Eco47I GGWCC 1 cut(s) 806
Eco53kI GAGCTC 1 cut(s) 991
Eco57I CTGAAG 2 cut(s) 51, 446
Eco88I CYCGRG 1 cut(s) 810
Eco91I GGTNACC 1 cut(s) 662
EcoICRI GAGCTC 1 cut(s) 991
EcoO109I RGGNCCY 1 cut(s) 806
EcoO65I GGTNACC 1 cut(s) 662
EcoRII CCWGG 3 cut(s) 394, 611, 658
EcoT38I GRGCYC 1 cut(s) 993
FaeI CATG 8 cut(s) 157, 346, 595, 611, 635, 742, 946, 1084
FaqI GGGAC 1 cut(s) 792
FatI CATG 8 cut(s) 153, 342, 591, 607, 631, 738, 942, 1080
FauNDI CATATG 1 cut(s) 930
FbaI TGATCA 1 cut(s) 319
Fnu4HI GCNGC 7 cut(s) 6, 111, 384, 387, 825, 962, 1025
FokI GGATG 3 cut(s) 419, 855, 1046
FriOI GRGCYC 1 cut(s) 993
Fsp4HI GCNGC 7 cut(s) 6, 111, 384, 387, 825, 962, 1025
FspBI CTAG 2 cut(s) 474, 744
GluI GCNGC 7 cut(s) 6, 111, 384, 387, 825, 962, 1025
GsaI CCCAGC 2 cut(s) 66, 671
HaeIII GGCC 2 cut(s) 60, 214
HapII CCGG 1 cut(s) 891
Hin1II CATG 8 cut(s) 157, 346, 595, 611, 635, 742, 946, 1084
HindIII AAGCTT 2 cut(s) 33, 146
HinfI GANTC 4 cut(s) 542, 776, 951, 1040
HpaII CCGG 1 cut(s) 891
Hpy166II GTNNAC 1 cut(s) 1057
Hpy188I TCNGA 2 cut(s) 31, 366
Hpy188III TCNNGA 1 cut(s) 474
Hpy8I GTNNAC 1 cut(s) 1057
HpyAV CCTTC 5 cut(s) 470, 693, 742, 941, 1042
HpyCH4III ACNGT 2 cut(s) 628, 880
HpyCH4V TGCA 4 cut(s) 140, 191, 607, 923
HpyF10VI GCNNNNNNNGC 3 cut(s) 572, 581, 604
HpyF3I CTNAG 4 cut(s) 205, 363, 538, 982
Hsp92II CATG 8 cut(s) 157, 346, 595, 611, 635, 742, 946, 1084
KflI GGGWCCC 1 cut(s) 806
Ksp22I TGATCA 1 cut(s) 319
Kzo9I GATC 2 cut(s) 319, 722
LguI GCTCTTC 3 cut(s) 353, 998, 1014
LmnI GCTCC 1 cut(s) 595
Lsp1109I GCAGC 6 cut(s) 17, 97, 370, 811, 973, 1036
LweI GCATC 3 cut(s) 97, 415, 932
MaeI CTAG 2 cut(s) 474, 744
MaeIII GTNAC 2 cut(s) 490, 662
MalI GATC 2 cut(s) 321, 724
MboI GATC 2 cut(s) 319, 722
MboII GAAGA 7 cut(s) 301, 340, 441, 946, 985, 986, 1031
MflI RGATCY 1 cut(s) 722
MhlI GDGCHC 3 cut(s) 600, 619, 993
MluCI AATT 6 cut(s) 102, 283, 299, 352, 714, 1087
MlyI GAGTC 3 cut(s) 536, 945, 1049
MroXI GAANNNNTTC 2 cut(s) 252, 898
MseI TTAA 3 cut(s) 315, 783, 1074
MslI CAYNNNNRTG 1 cut(s) 737
MspA1I CMGCKG 1 cut(s) 671
MspI CCGG 1 cut(s) 891
MspR9I CCNGG 4 cut(s) 396, 613, 660, 891
MvaI CCWGG 3 cut(s) 396, 613, 660
MwoI GCNNNNNNNGC 3 cut(s) 572, 581, 604
NciI CCSGG 1 cut(s) 891
NdeI CATATG 1 cut(s) 930
NdeII GATC 2 cut(s) 319, 722
NlaIII CATG 8 cut(s) 157, 346, 595, 611, 635, 742, 946, 1084
NlaIV GGNNCC 4 cut(s) 61, 807, 808, 888
NmeAIII GCCGAG 2 cut(s) 299, 610
NspI RCATGY 2 cut(s) 346, 611
PaeI GCATGC 1 cut(s) 611
PciSI GCTCTTC 3 cut(s) 353, 998, 1014
PdmI GAANNNNTTC 2 cut(s) 252, 898
PfeI GAWTC 1 cut(s) 776
PkrI GCNGC 7 cut(s) 7, 112, 385, 388, 826, 963, 1026
PleI GAGTC 3 cut(s) 536, 945, 1048
PpsI GAGTC 3 cut(s) 536, 945, 1048
PpuMI RGGWCCY 1 cut(s) 806
Psp124BI GAGCTC 1 cut(s) 993
Psp5II RGGWCCY 1 cut(s) 806
Psp6I CCWGG 3 cut(s) 394, 611, 658
PspEI GGTNACC 1 cut(s) 662
PspFI CCCAGC 2 cut(s) 62, 667
PspGI CCWGG 3 cut(s) 394, 611, 658
PspN4I GGNNCC 4 cut(s) 61, 807, 808, 888
PspPI GGNCC 3 cut(s) 59, 212, 806
PspPPI RGGWCCY 1 cut(s) 806
PsrI GAACNNNNNNTAC 2 cut(s) 71, 103
PsuI RGATCY 1 cut(s) 722
PvuII CAGCTG 1 cut(s) 671
RsaI GTAC 3 cut(s) 480, 630, 648
RsaNI GTAC 3 cut(s) 479, 629, 647
RseI CAYNNNNRTG 1 cut(s) 737
SacI GAGCTC 1 cut(s) 993
SapI GCTCTTC 3 cut(s) 353, 998, 1014
SaqAI TTAA 3 cut(s) 315, 783, 1074
SatI GCNGC 7 cut(s) 6, 111, 384, 387, 825, 962, 1025
Sau3AI GATC 2 cut(s) 319, 722
Sau96I GGNCC 3 cut(s) 59, 212, 806
SchI GAGTC 3 cut(s) 536, 945, 1049
ScrFI CCNGG 4 cut(s) 396, 613, 660, 891
SduI GDGCHC 3 cut(s) 600, 619, 993
SfaNI GCATC 3 cut(s) 97, 415, 932
SfcI CTRYAG 1 cut(s) 828
SinI GGWCC 1 cut(s) 806
SmiMI CAYNNNNRTG 1 cut(s) 737
SphI GCATGC 1 cut(s) 611
Sse9I AATT 6 cut(s) 102, 283, 299, 352, 714, 1087
SsiI CCGC 2 cut(s) 386, 1032
SspMI CTAG 2 cut(s) 474, 744
SstI GAGCTC 1 cut(s) 993
StyD4I CCNGG 4 cut(s) 394, 611, 658, 889
TaaI ACNGT 2 cut(s) 628, 880
TaqI TCGA 2 cut(s) 412, 996
TasI AATT 6 cut(s) 102, 283, 299, 352, 714, 1087
TatI WGTACW 1 cut(s) 628
TauI GCSGC 1 cut(s) 389
TfiI GAWTC 1 cut(s) 776
Tru1I TTAA 3 cut(s) 315, 783, 1074
Tru9I TTAA 3 cut(s) 315, 783, 1074
TscAI CASTG 1 cut(s) 470
TseI GCWGC 6 cut(s) 5, 110, 383, 824, 961, 1024
TspDTI ATGAA 4 cut(s) 59, 156, 567, 768
TspRI CASTG 1 cut(s) 470
VpaK11BI GGWCC 1 cut(s) 806
XapI RAATTY 2 cut(s) 299, 714
XbaI TCTAGA 1 cut(s) 473
XceI RCATGY 2 cut(s) 346, 611
XmnI GAANNNNTTC 2 cut(s) 252, 898
XspI CTAG 2 cut(s) 474, 744
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.