Rorug03G0324300

Sec-independent protein translocase protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0004134
Physical Location & Seq
Reverse (-)
35498513 .. 35500300
1788 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0324300.1

Sequence Viewer

Length: 810 bp
ATGCTCATCCACCAGAACGTCTTCAGAGTCCGAATCGCCGACCTCGGCCCCACCATCGATGCCGAACCTTCCGAGTCCAACGGAATCCTCGCCGATGCGTTGCACTCCGACCGCGCCGAGTACATTTCCATTGACCTTCGCTGCAAGGATCAAGTTCTTCAAGCATTTCAAGGTGCTGAGGTTGTGTTCCATATGGCGGCACCTAATTCGTCGATCAGCAATTACCAGCTCCACCATTCGGTCAATGTTGAAGGAACTAAGAATGTAATCGATGCTTGTGTTGAGCAGAAAGTGAAGAGGCTCATATACACTAGCTCTCCTAGCGTTGTGTTTGATGGGGTTCATGGCATTATTGATGGGGATGAGTCATTGCCGTATCCACCTAAGCACAATGATACTTATTCGGCCACTAAAGCGCAAGGAGAGGAGTCGGTACTAAAGGCAAACGGTGCTAAAGGGCTTCTAACCTACTGCATACACCCTAGCAGCATTTTCGGACCCGGTGATAGGTTACTTATTCTGTCTTTAGTTGTTACCGCCAGGGCAGGGAAATCCAAGTTCATTATAGGGGATGGCAATAACATTTATGATTTCACGTATGTTGAAAACGTGGCTCATGCCCATATATGTGCAGAGCGAGCTCTAGCATTTGAAGGGACAGTTGCAGAGAAAGCTGCAGGACAGGCATATTTTATAACCAATATGGAACCTATCAAGTTTTGGGAGTTTATGTCACTTATTCTAGAAGGTCTTGGCTATGAAAGGCCCATATGGGATGAAGATTCCACAATTGACGCCTTCAAGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

269

Amino Acids

29.56

Weight (kDa)

5.15

Isoelectric Point (pI)

30.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
3Beta_HSD PF01073 7 - 256 2.1e-65 3-beta hydroxysteroid dehydrogenase/isomerase family
Epimerase PF01370 34 - 213 2.3e-27 NAD dependent epimerase/dehydratase family
NAD_binding_10 PF13460 34 - 110 1.3e-08 NAD(P)H-binding
GDP_Man_Dehyd PF16363 36 - 209 5.9e-10 GDP-mannose 4,6 dehydratase
RmlD_sub_bind PF04321 43 - 253 1e-07 RmlD substrate binding domain
Polysacc_synt_2 PF02719 43 - 106 8.8e-06 Polysaccharide biosynthesis protein
NAD_binding_4 PF07993 56 - 206 8.2e-12 Male sterility protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 695
AccB1I GGYRCC 1 cut(s) 199
AccII CGCG 1 cut(s) 114
AciI CCGC 3 cut(s) 112, 197, 537
AclWI GGATC 1 cut(s) 156
AcoI YGGCCR 1 cut(s) 405
AcuI CTGAAG 1 cut(s) 7
AcyI GRCGYC 1 cut(s) 795
AfaI GTAC 2 cut(s) 122, 435
AfiI CCNNNNNNNGG 4 cut(s) 196, 238, 507, 546
AgsI TTSAA 6 cut(s) 161, 170, 251, 605, 653, 802
AjnI CCWGG 1 cut(s) 539
AluBI AGCT 4 cut(s) 229, 315, 641, 674
AluI AGCT 4 cut(s) 229, 315, 641, 674
Alw21I GWGCWC 1 cut(s) 643
AlwI GGATC 1 cut(s) 156
AoxI GGCC 3 cut(s) 46, 405, 764
ApeKI GCWGC 3 cut(s) 141, 486, 674
Asp700I GAANNNNTTC 1 cut(s) 20
AspLEI GCGC 2 cut(s) 116, 418
AspS9I GGNCC 3 cut(s) 47, 497, 765
AsuC2I CCSGG 1 cut(s) 501
AsuHPI GGTGA 1 cut(s) 515
AvaII GGWCC 1 cut(s) 497
BanI GGYRCC 1 cut(s) 199
BanII GRGCYC 1 cut(s) 643
BbsI GAAGAC 1 cut(s) 13
Bbv12I GWGCWC 1 cut(s) 643
BbvCI CCTCAGC 1 cut(s) 177
BbvI GCAGC 3 cut(s) 128, 498, 661
BccI CCATC 4 cut(s) 62, 329, 350, 566
BceAI ACGGC 1 cut(s) 358
BcgI CGANNNNNNTGC 4 cut(s) 189, 223, 475, 509
BciT130I CCWGG 1 cut(s) 541
BciVI GTATCC 1 cut(s) 387
BcnI CCSGG 1 cut(s) 501
BfaI CTAG 5 cut(s) 312, 321, 483, 644, 743
BfmI CTRYAG 1 cut(s) 675
BfuI GTATCC 1 cut(s) 387
BisI GCNGC 4 cut(s) 142, 198, 487, 675
BlsI GCNGC 4 cut(s) 143, 199, 488, 676
Bme1390I CCNGG 2 cut(s) 501, 541
Bme18I GGWCC 1 cut(s) 497
BmgT120I GGNCC 3 cut(s) 47, 497, 765
BmiI GGNNCC 4 cut(s) 49, 201, 499, 708
BmrFI CCNGG 2 cut(s) 501, 541
BmsI GCATC 3 cut(s) 49, 85, 262
BpiI GAAGAC 1 cut(s) 13
Bpu10I CCTNAGC 2 cut(s) 177, 384
BpuMI CCSGG 1 cut(s) 501
Bsa29I ATCGAT 2 cut(s) 57, 270
BsaAI YACGTR 1 cut(s) 597
BsaHI GRCGYC 1 cut(s) 795
BsaJI CCNNGG 2 cut(s) 43, 540
BsaXI ACNNNNNCTCC 4 cut(s) 301, 331, 716, 746
Bsc4I CCNNNNNNNGG 4 cut(s) 196, 238, 507, 546
Bse3DI GCAATG 1 cut(s) 368
BseBI CCWGG 1 cut(s) 541
BseCI ATCGAT 2 cut(s) 57, 270
BseDI CCNNGG 2 cut(s) 43, 540
BseGI GGATG 4 cut(s) 6, 367, 577, 781
BseLI CCNNNNNNNGG 4 cut(s) 196, 238, 507, 546
BseMI GCAATG 1 cut(s) 368
BseMII CTCAG 1 cut(s) 168
BseRI GAGGAG 1 cut(s) 440
BseXI GCAGC 3 cut(s) 128, 498, 661
BsgI GTGCAG 1 cut(s) 651
Bsh1236I CGCG 1 cut(s) 114
Bsh1285I CGRYCG 1 cut(s) 112
BshFI GGCC 3 cut(s) 48, 407, 766
BshNI GGYRCC 1 cut(s) 199
BshVI ATCGAT 2 cut(s) 57, 270
BsiEI CGRYCG 1 cut(s) 112
BsiHKAI GWGCWC 1 cut(s) 643
BsiSI CCGG 1 cut(s) 501
BslFI GGGAC 1 cut(s) 670
BslI CCNNNNNNNGG 4 cut(s) 196, 238, 507, 546
BsmFI GGGAC 1 cut(s) 670
BsnI GGCC 3 cut(s) 48, 407, 766
Bsp1286I GDGCHC 1 cut(s) 643
Bsp143I GATC 2 cut(s) 148, 213
BspACI CCGC 3 cut(s) 112, 197, 537
BspANI GGCC 3 cut(s) 48, 407, 766
BspCNI CTCAG 1 cut(s) 169
BspDI ATCGAT 2 cut(s) 57, 270
BspFNI CGCG 1 cut(s) 114
BspLI GGNNCC 4 cut(s) 49, 201, 499, 708
BspMAI CTGCAG 1 cut(s) 679
BspPI GGATC 1 cut(s) 156
BspT107I GGYRCC 1 cut(s) 199
BsrDI GCAATG 1 cut(s) 368
BssECI CCNNGG 2 cut(s) 43, 540
BssMI GATC 2 cut(s) 148, 213
BssNI GRCGYC 1 cut(s) 795
Bst2UI CCWGG 1 cut(s) 541
Bst4CI ACNGT 2 cut(s) 449, 661
Bst6I CTCTTC 1 cut(s) 290
BstACI GRCGYC 1 cut(s) 795
BstAPI GCANNNNNTGC 1 cut(s) 449
BstBAI YACGTR 1 cut(s) 597
BstC8I GCNNGC 1 cut(s) 639
BstDEI CTNAG 3 cut(s) 177, 258, 384
BstF5I GGATG 4 cut(s) 6, 367, 577, 781
BstFNI CGCG 1 cut(s) 114
BstHHI GCGC 2 cut(s) 116, 418
BstKTI GATC 2 cut(s) 151, 216
BstMBI GATC 2 cut(s) 148, 213
BstMCI CGRYCG 1 cut(s) 112
BstMWI GCNNNNNNNGC 6 cut(s) 321, 413, 449, 638, 671, 683
BstNI CCWGG 1 cut(s) 541
BstSCI CCNGG 2 cut(s) 499, 539
BstSFI CTRYAG 1 cut(s) 675
BstUI CGCG 1 cut(s) 114
BstV1I GCAGC 3 cut(s) 128, 498, 661
BstV2I GAAGAC 1 cut(s) 13
Bsu15I ATCGAT 2 cut(s) 57, 270
BsuI GTATCC 1 cut(s) 387
BsuRI GGCC 3 cut(s) 48, 407, 766
BsuTUI ATCGAT 2 cut(s) 57, 270
BtsCI GGATG 4 cut(s) 6, 367, 577, 781
Cac8I GCNNGC 1 cut(s) 639
CfoI GCGC 2 cut(s) 116, 418
Cfr13I GGNCC 3 cut(s) 47, 497, 765
ClaI ATCGAT 2 cut(s) 57, 270
CseI GACGC 1 cut(s) 803
Csp6I GTAC 2 cut(s) 121, 434
CviAII CATG 2 cut(s) 344, 617
CviQI GTAC 2 cut(s) 121, 434
DdeI CTNAG 3 cut(s) 177, 258, 384
DpnI GATC 2 cut(s) 150, 215
DpnII GATC 2 cut(s) 148, 213
EaeI YGGCCR 1 cut(s) 405
Eam1104I CTCTTC 1 cut(s) 290
EarI CTCTTC 1 cut(s) 290
Ecl136II GAGCTC 1 cut(s) 641
Eco24I GRGCYC 1 cut(s) 643
Eco47I GGWCC 1 cut(s) 497
Eco53kI GAGCTC 1 cut(s) 641
Eco57I CTGAAG 1 cut(s) 7
EcoICRI GAGCTC 1 cut(s) 641
EcoRII CCWGG 1 cut(s) 539
EcoT38I GRGCYC 1 cut(s) 643
FaeI CATG 2 cut(s) 347, 620
FaqI GGGAC 1 cut(s) 670
FatI CATG 2 cut(s) 343, 616
FauNDI CATATG 2 cut(s) 192, 770
Fnu4HI GCNGC 4 cut(s) 142, 198, 487, 675
FokI GGATG 3 cut(s) 374, 584, 788
FriOI GRGCYC 1 cut(s) 643
Fsp4HI GCNGC 4 cut(s) 142, 198, 487, 675
FspBI CTAG 5 cut(s) 312, 321, 483, 644, 743
GlaI GCGC 2 cut(s) 115, 417
GluI GCNGC 4 cut(s) 142, 198, 487, 675
HaeIII GGCC 3 cut(s) 48, 407, 766
HapII CCGG 1 cut(s) 501
HgaI GACGC 1 cut(s) 803
HhaI GCGC 2 cut(s) 116, 418
Hin1I GRCGYC 1 cut(s) 795
Hin1II CATG 2 cut(s) 347, 620
Hin6I GCGC 2 cut(s) 114, 416
HinP1I GCGC 2 cut(s) 114, 416
HinfI GANTC 7 cut(s) 27, 33, 74, 84, 365, 428, 782
HpaII CCGG 1 cut(s) 501
HphI GGTGA 1 cut(s) 515
Hpy188I TCNGA 5 cut(s) 26, 32, 73, 109, 497
Hpy188III TCNNGA 2 cut(s) 743, 802
Hpy99I CGWCG 1 cut(s) 214
HpyAV CCTTC 6 cut(s) 78, 146, 245, 647, 740, 808
HpyCH4III ACNGT 2 cut(s) 449, 661
HpyCH4IV ACGT 3 cut(s) 18, 596, 609
HpyCH4V TGCA 6 cut(s) 103, 144, 474, 632, 665, 677
HpyF10VI GCNNNNNNNGC 6 cut(s) 321, 413, 449, 638, 671, 683
HpyF3I CTNAG 3 cut(s) 177, 258, 384
HpySE526I ACGT 3 cut(s) 18, 596, 609
Hsp92I GRCGYC 1 cut(s) 795
Hsp92II CATG 2 cut(s) 347, 620
HspAI GCGC 2 cut(s) 114, 416
Kzo9I GATC 2 cut(s) 148, 213
LmnI GCTCC 1 cut(s) 234
LpnPI CCDG 8 cut(s) 26, 239, 514, 526, 531, 553, 663, 668
Lsp1109I GCAGC 3 cut(s) 128, 498, 661
LweI GCATC 3 cut(s) 49, 85, 262
MaeI CTAG 5 cut(s) 312, 321, 483, 644, 743
MaeII ACGT 3 cut(s) 18, 596, 609
MaeIII GTNAC 3 cut(s) 510, 532, 732
MalI GATC 2 cut(s) 150, 215
MboI GATC 2 cut(s) 148, 213
MboII GAAGA 4 cut(s) 13, 149, 307, 791
MfeI CAATTG 1 cut(s) 789
MhlI GDGCHC 1 cut(s) 643
MluCI AATT 4 cut(s) 205, 220, 789, 805
MlyI GAGTC 4 cut(s) 36, 83, 374, 437
MmeI TCCRAC 2 cut(s) 102, 132
MnlI CCTC 5 cut(s) 53, 98, 172, 291, 418
MroXI GAANNNNTTC 1 cut(s) 20
MslI CAYNNNNRTG 1 cut(s) 627
MspI CCGG 1 cut(s) 501
MspR9I CCNGG 2 cut(s) 501, 541
MunI CAATTG 1 cut(s) 789
MvaI CCWGG 1 cut(s) 541
MvnI CGCG 1 cut(s) 114
MwoI GCNNNNNNNGC 6 cut(s) 321, 413, 449, 638, 671, 683
NciI CCSGG 1 cut(s) 501
NdeI CATATG 2 cut(s) 192, 770
NdeII GATC 2 cut(s) 148, 213
NlaIII CATG 2 cut(s) 347, 620
NlaIV GGNNCC 4 cut(s) 49, 201, 499, 708
NmeAIII GCCGAG 2 cut(s) 24, 142
NmuCI GTSAC 1 cut(s) 732
PdmI GAANNNNTTC 1 cut(s) 20
PfeI GAWTC 3 cut(s) 33, 84, 782
PkrI GCNGC 4 cut(s) 143, 199, 488, 676
PleI GAGTC 4 cut(s) 35, 82, 373, 436
PpsI GAGTC 4 cut(s) 35, 82, 373, 436
Ppu21I YACGTR 1 cut(s) 597
PsiI TTATAA 1 cut(s) 695
Psp124BI GAGCTC 1 cut(s) 643
Psp6I CCWGG 1 cut(s) 539
PspGI CCWGG 1 cut(s) 539
PspN4I GGNNCC 4 cut(s) 49, 201, 499, 708
PspPI GGNCC 3 cut(s) 47, 497, 765
PstI CTGCAG 1 cut(s) 679
RsaI GTAC 2 cut(s) 122, 435
RsaNI GTAC 2 cut(s) 121, 434
RseI CAYNNNNRTG 1 cut(s) 627
SacI GAGCTC 1 cut(s) 643
SatI GCNGC 4 cut(s) 142, 198, 487, 675
Sau3AI GATC 2 cut(s) 148, 213
Sau96I GGNCC 3 cut(s) 47, 497, 765
SchI GAGTC 4 cut(s) 36, 83, 374, 437
ScrFI CCNGG 2 cut(s) 501, 541
SduI GDGCHC 1 cut(s) 643
SfaNI GCATC 3 cut(s) 49, 85, 262
SfcI CTRYAG 1 cut(s) 675
SinI GGWCC 1 cut(s) 497
SmiMI CAYNNNNRTG 1 cut(s) 627
Sse9I AATT 4 cut(s) 205, 220, 789, 805
SsiI CCGC 3 cut(s) 112, 197, 537
SspMI CTAG 5 cut(s) 312, 321, 483, 644, 743
SstI GAGCTC 1 cut(s) 643
StyD4I CCNGG 2 cut(s) 499, 539
TaaI ACNGT 2 cut(s) 449, 661
TaiI ACGT 3 cut(s) 21, 599, 612
TaqI TCGA 3 cut(s) 57, 212, 270
TaqII GACCGA 1 cut(s) 229
TasI AATT 4 cut(s) 205, 220, 789, 805
TatI WGTACW 1 cut(s) 120
TauI GCSGC 1 cut(s) 200
TfiI GAWTC 3 cut(s) 33, 84, 782
TseFI GTSAC 1 cut(s) 732
TseI GCWGC 3 cut(s) 141, 486, 674
Tsp45I GTSAC 1 cut(s) 732
TspDTI ATGAA 4 cut(s) 332, 550, 774, 792
TspGWI ACGGA 1 cut(s) 96
VpaK11BI GGWCC 1 cut(s) 497
XbaI TCTAGA 1 cut(s) 742
XmnI GAANNNNTTC 1 cut(s) 20
XspI CTAG 5 cut(s) 312, 321, 483, 644, 743
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.