Rorug04G0014900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0004071
Physical Location & Seq
Forward (+)
2378089 .. 2384086
5998 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0014900.1

Sequence Viewer

Length: 1659 bp
ATGTACGAGAACCAGAAAGATATGTATGATAATGAATCTAATGTAGTGGTTGGCTTGAAGTTTGTGCGCAATGCATTGAATCATTTCAACCAGACCGGGAGGGAACTCAAGTACAAGGACTTATTTGGAAGAGTATATGTAAAAATACAGTTTTTCAAACATTTTGGTAATCAAGCGCTTCCTCTGTCTCATATTCTTCTGGAGCTAAAAAAGATGTATGGACTGAAGCTCATCTGTGAAGCATCTTTTAGAGGAAAAAAGGCTTGCTTGGAAATTAAAGCCAGTCACCGTGTTCAGAAGACAGAGGGTTTGATTAAGAAGCATGGTTACCGAAAAAGTGATAGTTGTGAGTTTTGGACCGCTGCAATGCCATGCCAAATCATTGTGTATCCTCATGCTTGTACATATGAGCATCAGTATAATATGCTTCTTGGTCTAGCTGCAAAAATGGCTAAAAGAGCCATTCAGACTTCTGGTAAGGACTTCAGTACTCTGTTATGGTTTGATTATACTGGGAGAGAGCCTGCAGATTGCCAGCCAAAACTTGCACTGATGCTTTTCCGATTACTAAAGATTGCTGAGGAATTTAATGTTGCTGTCTACATTACCAACGAAGCTGACCATGGCGGAGTATTATTCATTTCTGATCCAAAGAAACCAGCAGGAGGTCATGTGCTTGCCCATGCAGCGACCACAAAGCTCATCTTTAGAAAAGGCAAAGGGGAACAGCGTGTCTGCAAGGTGTTTGATGACCTAGACCTGCCTAAGCCTGAAGCGATATCCTTCTCATCTGCCTACGTCACCGAACCTCATAAGCACATGCGCCAAAATGGGCACCTTCGAAAGCTTACACCTTGCTCGAAAAGCCTTCAGTTCCTTGAAGAAGAAGAAGAAGAAGAAACAAAGGGTGTATTGTTTATTGGGAATAATACTTTGATTCATTTCTATGCAGAATGCGGGGACTTGGATTATGCCCGGAAGGTGTTTGATGAAATGCTTGAGAGAAACACTGTGTCGTGGACTAGTTTGATTTGTGGTTATGGTTGGAGGAACATGCCAAAGGAGGCTGTGAGTTCCAAAGCTTTCTTTGAGGTTGTTCCTAAAACAACAGAAGATGTCCGAGCCCTGTGCACGGTGGGGACTATAGATTTTCCATTCAGAAAGGTTACACAATCTGGCCAAGTTGGCTACATGCTGATTGCTGATCTAACTGAATTTGAGACGACCGCCAATGAGGTAGAAGCAAAGATGTGTGCTAACCTTTGTGAGGAAGAAGAATTGTTTTTCATTGTGTCTAAGGATGGAATTGATACTCTTAAACTCAGACGTTACAGAAACAATGAACGAATCTTTGTGAATCCTAATGAATGCAGTGATACTAAGATTAATCACAGTCATGCCAGGGTAGTGCAGTGGGGTGCTCCACCAAGAAGCTGTCTTAAATTGAATATTGATGGAGGCTCGAAGGGCGGCTTCTCTAGGCATCTTCCTGCAATCCAAAGCGAGGCAGAAGCTTGTAGAGCAGGCTTGCCAGTGGCTATTCACCAGAGTTGGAGACATGTTGAGGTAGAAAGTGATTGTGCTCAGTTTCTTGCTGTGCTGAATCTTCAAGGGGAGGATAACTCTGAGGAAAATTGTAGAATTGCAATCGAGGCATGA

Protein Analysis

552

Amino Acids

62.67

Weight (kDa)

7.25

Isoelectric Point (pI)

41.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad51 PF08423 127 - 260 4.5e-30 Rad51
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 68
Acc36I ACCTGC 1 cut(s) 768
AccB1I GGYRCC 1 cut(s) 834
AccI GTMKAC 1 cut(s) 600
AciI CCGC 5 cut(s) 360, 627, 957, 1227, 1470
AclWI GGATC 1 cut(s) 641
AcoI YGGCCR 1 cut(s) 1177
AcsI RAATTY 2 cut(s) 584, 1214
AcuI CTGAAG 4 cut(s) 245, 469, 792, 854
AfaI GTAC 4 cut(s) 5, 113, 403, 490
AfeI AGCGCT 1 cut(s) 177
AfiI CCNNNNNNNGG 4 cut(s) 665, 1132, 1267, 1504
AflIII ACRYGT 1 cut(s) 1558
AgsI TTSAA 7 cut(s) 58, 79, 88, 157, 881, 1447, 1610
AhlI ACTAGT 1 cut(s) 1022
AjnI CCWGG 1 cut(s) 1400
AluBI AGCT 9 cut(s) 205, 229, 440, 617, 700, 847, 1082, 1434, 1514
AluI AGCT 9 cut(s) 205, 229, 440, 617, 700, 847, 1082, 1434, 1514
Alw21I GWGCWC 3 cut(s) 1133, 1423, 1585
Alw26I GTCTC 3 cut(s) 192, 1214, 1549
Alw44I GTGCAC 1 cut(s) 1129
AlwI GGATC 1 cut(s) 641
Aor51HI AGCGCT 1 cut(s) 177
AoxI GGCC 1 cut(s) 1177
ApaLI GTGCAC 1 cut(s) 1129
ApeKI GCWGC 3 cut(s) 362, 440, 686
ApoI RAATTY 2 cut(s) 584, 1214
ArsI GACNNNNNNTTYG 2 cut(s) 292, 324
AseI ATTAAT 1 cut(s) 1386
Asp700I GAANNNNTTC 1 cut(s) 83
AspLEI GCGC 3 cut(s) 69, 178, 825
AspS9I GGNCC 1 cut(s) 357
AsuC2I CCSGG 2 cut(s) 97, 976
AsuHPI GGTGA 3 cut(s) 278, 793, 1535
AsuII TTCGAA 1 cut(s) 841
AvaII GGWCC 1 cut(s) 357
BaeGI GKGCMC 2 cut(s) 837, 1133
BalI TGGCCA 1 cut(s) 1179
BanI GGYRCC 1 cut(s) 834
BanII GRGCYC 1 cut(s) 1126
BarI GAAGNNNNNNTAC 2 cut(s) 311, 343
BbsI GAAGAC 1 cut(s) 305
Bbv12I GWGCWC 3 cut(s) 1133, 1423, 1585
BbvCI CCTCAGC 1 cut(s) 579
BbvI GCAGC 3 cut(s) 349, 427, 698
BccI CCATC 2 cut(s) 1295, 1448
BcgI CGANNNNNNTGC 2 cut(s) 1110, 1144
BciT130I CCWGG 1 cut(s) 1402
BciVI GTATCC 1 cut(s) 399
BcnI CCSGG 2 cut(s) 97, 976
BcoDI GTCTC 3 cut(s) 192, 1214, 1549
BcuI ACTAGT 1 cut(s) 1022
BfaI CTAG 4 cut(s) 437, 755, 1023, 1479
BfmI CTRYAG 2 cut(s) 525, 1143
BfoI RGCGCY 1 cut(s) 179
BfuAI ACCTGC 1 cut(s) 768
BfuI GTATCC 1 cut(s) 399
BglI GCCNNNNNGGC 1 cut(s) 1185
BisI GCNGC 4 cut(s) 363, 441, 687, 1471
BlsI GCNGC 4 cut(s) 364, 442, 688, 1472
BmcAI AGTACT 1 cut(s) 490
Bme1390I CCNGG 3 cut(s) 97, 976, 1402
Bme18I GGWCC 1 cut(s) 357
BmgT120I GGNCC 1 cut(s) 357
BmiI GGNNCC 1 cut(s) 836
BmrFI CCNGG 3 cut(s) 97, 976, 1402
BmrI ACTGGG 1 cut(s) 522
BmsI GCATC 4 cut(s) 251, 421, 543, 1492
BmuI ACTGGG 1 cut(s) 522
BpiI GAAGAC 1 cut(s) 305
BplI GAGNNNNNCTC 2 cut(s) 1607, 1639
BpmI CTGGAG 1 cut(s) 221
Bpu10I CCTNAGC 2 cut(s) 579, 765
Bpu14I TTCGAA 1 cut(s) 841
BpuEI CTTGAG 2 cut(s) 92, 1019
BpuMI CCSGG 2 cut(s) 97, 976
BsaJI CCNNGG 2 cut(s) 622, 1401
BsaXI ACNNNNNCTCC 2 cut(s) 657, 687
Bsc4I CCNNNNNNNGG 4 cut(s) 665, 1132, 1267, 1504
Bse1I ACTGG 3 cut(s) 282, 517, 1532
Bse3DI GCAATG 2 cut(s) 76, 372
BseBI CCWGG 1 cut(s) 1402
BseDI CCNNGG 2 cut(s) 622, 1401
BseGI GGATG 1 cut(s) 1306
BseLI CCNNNNNNNGG 4 cut(s) 665, 1132, 1267, 1504
BseMI GCAATG 2 cut(s) 76, 372
BseMII CTCAG 4 cut(s) 570, 1336, 1598, 1617
BseNI ACTGG 3 cut(s) 282, 517, 1532
BseSI GKGCMC 2 cut(s) 837, 1133
BseXI GCAGC 3 cut(s) 349, 427, 698
BsgI GTGCAG 1 cut(s) 1430
Bsh1285I CGRYCG 1 cut(s) 1227
BshFI GGCC 1 cut(s) 1179
BshNI GGYRCC 1 cut(s) 834
BsiEI CGRYCG 1 cut(s) 1227
BsiHKAI GWGCWC 3 cut(s) 1133, 1423, 1585
BsiSI CCGG 2 cut(s) 96, 976
BslFI GGGAC 2 cut(s) 974, 1153
BslI CCNNNNNNNGG 4 cut(s) 665, 1132, 1267, 1504
BsmAI GTCTC 3 cut(s) 192, 1214, 1549
BsmBI CGTCTC 1 cut(s) 1214
BsmFI GGGAC 2 cut(s) 974, 1153
BsmI GAATGC 2 cut(s) 959, 1373
BsnI GGCC 1 cut(s) 1179
Bsp119I TTCGAA 1 cut(s) 841
Bsp1286I GDGCHC 5 cut(s) 837, 1126, 1133, 1423, 1585
Bsp1407I TGTACA 1 cut(s) 401
Bsp143I GATC 2 cut(s) 646, 1204
Bsp19I CCATGG 1 cut(s) 622
BspACI CCGC 5 cut(s) 360, 627, 957, 1227, 1470
BspANI GGCC 1 cut(s) 1179
BspCNI CTCAG 4 cut(s) 571, 1335, 1597, 1618
BspLI GGNNCC 1 cut(s) 836
BspMAI CTGCAG 1 cut(s) 529
BspMI ACCTGC 1 cut(s) 768
BspPI GGATC 1 cut(s) 641
BspT104I TTCGAA 1 cut(s) 841
BspT107I GGYRCC 1 cut(s) 834
BsrDI GCAATG 2 cut(s) 76, 372
BsrGI TGTACA 1 cut(s) 401
BsrI ACTGG 3 cut(s) 282, 517, 1532
BssECI CCNNGG 2 cut(s) 622, 1401
BssMI GATC 2 cut(s) 646, 1204
BssT1I CCWWGG 1 cut(s) 622
Bst2UI CCWGG 1 cut(s) 1402
Bst4CI ACNGT 5 cut(s) 150, 290, 1012, 1135, 1394
Bst6I CTCTTC 1 cut(s) 124
BstAUI TGTACA 1 cut(s) 401
BstBI TTCGAA 1 cut(s) 841
BstC8I GCNNGC 6 cut(s) 265, 525, 536, 678, 1525, 1529
BstDEI CTNAG 7 cut(s) 579, 765, 1296, 1322, 1380, 1584, 1626
BstDSI CCRYGG 1 cut(s) 622
BstEII GGTNACC 1 cut(s) 326
BstENI CCTNNNNNAGG 1 cut(s) 1265
BstF5I GGATG 1 cut(s) 1306
BstH2I RGCGCY 1 cut(s) 179
BstHHI GCGC 3 cut(s) 69, 178, 825
BstKTI GATC 2 cut(s) 649, 1207
BstMAI GTCTC 3 cut(s) 192, 1214, 1549
BstMBI GATC 2 cut(s) 646, 1204
BstMCI CGRYCG 1 cut(s) 1227
BstMWI GCNNNNNNNGC 8 cut(s) 449, 458, 686, 864, 1185, 1467, 1520, 1652
BstNI CCWGG 1 cut(s) 1402
BstNSI RCATGY 4 cut(s) 823, 1057, 1195, 1562
BstPI GGTNACC 1 cut(s) 326
BstSCI CCNGG 3 cut(s) 95, 974, 1400
BstSFI CTRYAG 2 cut(s) 525, 1143
BstSLI GKGCMC 2 cut(s) 837, 1133
BstV1I GCAGC 3 cut(s) 349, 427, 698
BstV2I GAAGAC 1 cut(s) 305
BsuI GTATCC 1 cut(s) 399
BsuRI GGCC 1 cut(s) 1179
BtgI CCRYGG 1 cut(s) 622
BtsCI GGATG 1 cut(s) 1306
BtsI GCAGTG 2 cut(s) 1378, 1418
BtsIMutI CAGTG 5 cut(s) 548, 1008, 1378, 1418, 1539
BveI ACCTGC 1 cut(s) 768
Cac8I GCNNGC 6 cut(s) 265, 525, 536, 678, 1525, 1529
CfoI GCGC 3 cut(s) 69, 178, 825
Cfr13I GGNCC 1 cut(s) 357
Csp6I GTAC 4 cut(s) 4, 112, 402, 489
CviQI GTAC 4 cut(s) 4, 112, 402, 489
DdeI CTNAG 7 cut(s) 579, 765, 1296, 1322, 1380, 1584, 1626
DpnI GATC 2 cut(s) 648, 1206
DpnII GATC 2 cut(s) 646, 1204
EaeI YGGCCR 1 cut(s) 1177
Eam1104I CTCTTC 1 cut(s) 124
EarI CTCTTC 1 cut(s) 124
EciI GGCGGA 1 cut(s) 642
Eco130I CCWWGG 1 cut(s) 622
Eco24I GRGCYC 1 cut(s) 1126
Eco32I GATATC 1 cut(s) 780
Eco47I GGWCC 1 cut(s) 357
Eco47III AGCGCT 1 cut(s) 177
Eco57I CTGAAG 4 cut(s) 245, 469, 792, 854
Eco91I GGTNACC 1 cut(s) 326
EcoNI CCTNNNNNAGG 1 cut(s) 1265
EcoO65I GGTNACC 1 cut(s) 326
EcoRII CCWGG 1 cut(s) 1400
EcoRV GATATC 1 cut(s) 780
EcoT14I CCWWGG 1 cut(s) 622
EcoT22I ATGCAT 1 cut(s) 76
EcoT38I GRGCYC 1 cut(s) 1126
ErhI CCWWGG 1 cut(s) 622
Esp3I CGTCTC 1 cut(s) 1214
FalI AAGNNNNNCTT 6 cut(s) 251, 283, 689, 721, 1457, 1489
FaqI GGGAC 2 cut(s) 974, 1153
FauI CCCGC 1 cut(s) 950
FauNDI CATATG 1 cut(s) 406
FblI GTMKAC 1 cut(s) 600
Fnu4HI GCNGC 4 cut(s) 363, 441, 687, 1471
FokI GGATG 1 cut(s) 1313
FriOI GRGCYC 1 cut(s) 1126
Fsp4HI GCNGC 4 cut(s) 363, 441, 687, 1471
FspBI CTAG 4 cut(s) 437, 755, 1023, 1479
FspI TGCGCA 1 cut(s) 68
GlaI GCGC 3 cut(s) 68, 177, 824
GluI GCNGC 4 cut(s) 363, 441, 687, 1471
GsuI CTGGAG 1 cut(s) 221
HaeII RGCGCY 1 cut(s) 179
HaeIII GGCC 1 cut(s) 1179
HapII CCGG 2 cut(s) 96, 976
HhaI GCGC 3 cut(s) 69, 178, 825
Hin6I GCGC 3 cut(s) 67, 176, 823
HinP1I GCGC 3 cut(s) 67, 176, 823
HindIII AAGCTT 3 cut(s) 845, 1080, 1512
HinfI GANTC 6 cut(s) 35, 79, 937, 1347, 1357, 1603
HpaII CCGG 2 cut(s) 96, 976
HphI GGTGA 3 cut(s) 278, 793, 1535
Hpy166II GTNNAC 3 cut(s) 601, 1020, 1131
Hpy188I TCNGA 8 cut(s) 297, 468, 563, 646, 1121, 1160, 1325, 1627
Hpy188III TCNNGA 1 cut(s) 200
Hpy8I GTNNAC 3 cut(s) 601, 1020, 1131
HpyAV CCTTC 5 cut(s) 793, 848, 878, 973, 1459
HpyCH4III ACNGT 5 cut(s) 150, 290, 1012, 1135, 1394
HpyCH4IV ACGT 2 cut(s) 798, 1327
HpyF10VI GCNNNNNNNGC 8 cut(s) 449, 458, 686, 864, 1185, 1467, 1520, 1652
HpyF3I CTNAG 7 cut(s) 579, 765, 1296, 1322, 1380, 1584, 1626
HpySE526I ACGT 2 cut(s) 798, 1327
HspAI GCGC 3 cut(s) 67, 176, 823
Kzo9I GATC 2 cut(s) 646, 1204
LmnI GCTCC 2 cut(s) 202, 1426
Lsp1109I GCAGC 3 cut(s) 349, 427, 698
LweI GCATC 4 cut(s) 251, 421, 543, 1492
MaeI CTAG 4 cut(s) 437, 755, 1023, 1479
MaeII ACGT 2 cut(s) 798, 1327
MaeIII GTNAC 5 cut(s) 284, 326, 799, 1165, 1328
MalI GATC 2 cut(s) 648, 1206
MboI GATC 2 cut(s) 646, 1204
MhlI GDGCHC 5 cut(s) 837, 1126, 1133, 1423, 1585
MlsI TGGCCA 1 cut(s) 1179
MluCI AATT 8 cut(s) 273, 584, 1214, 1277, 1305, 1442, 1633, 1641
MluNI TGGCCA 1 cut(s) 1179
MmeI TCCRAC 2 cut(s) 1025, 1532
Mox20I TGGCCA 1 cut(s) 1179
Mph1103I ATGCAT 1 cut(s) 76
MroXI GAANNNNTTC 1 cut(s) 83
MscI TGGCCA 1 cut(s) 1179
MseI TTAA 6 cut(s) 276, 315, 588, 1317, 1386, 1440
MslI CAYNNNNRTG 2 cut(s) 945, 1395
Msp20I TGGCCA 1 cut(s) 1179
MspA1I CMGCKG 1 cut(s) 362
MspI CCGG 2 cut(s) 96, 976
MspR9I CCNGG 3 cut(s) 97, 976, 1402
Mva1269I GAATGC 2 cut(s) 959, 1373
MvaI CCWGG 1 cut(s) 1402
MwoI GCNNNNNNNGC 8 cut(s) 449, 458, 686, 864, 1185, 1467, 1520, 1652
NciI CCSGG 2 cut(s) 97, 976
NcoI CCATGG 1 cut(s) 622
NdeI CATATG 1 cut(s) 406
NdeII GATC 2 cut(s) 646, 1204
NlaIV GGNNCC 1 cut(s) 836
NmuCI GTSAC 2 cut(s) 284, 799
NsbI TGCGCA 1 cut(s) 68
NsiI ATGCAT 1 cut(s) 76
NspI RCATGY 4 cut(s) 823, 1057, 1195, 1562
NspV TTCGAA 1 cut(s) 841
PciI ACATGT 1 cut(s) 1558
PctI GAATGC 2 cut(s) 959, 1373
PdmI GAANNNNTTC 1 cut(s) 83
PfeI GAWTC 6 cut(s) 35, 79, 937, 1347, 1357, 1603
PkrI GCNGC 4 cut(s) 364, 442, 688, 1472
PscI ACATGT 1 cut(s) 1558
PshBI ATTAAT 1 cut(s) 1386
Psp6I CCWGG 1 cut(s) 1400
PspEI GGTNACC 1 cut(s) 326
PspGI CCWGG 1 cut(s) 1400
PspN4I GGNNCC 1 cut(s) 836
PspPI GGNCC 1 cut(s) 357
PstI CTGCAG 1 cut(s) 529
RsaI GTAC 4 cut(s) 5, 113, 403, 490
RsaNI GTAC 4 cut(s) 4, 112, 402, 489
RseI CAYNNNNRTG 2 cut(s) 945, 1395
SaqAI TTAA 6 cut(s) 276, 315, 588, 1317, 1386, 1440
SatI GCNGC 4 cut(s) 363, 441, 687, 1471
Sau3AI GATC 2 cut(s) 646, 1204
Sau96I GGNCC 1 cut(s) 357
ScaI AGTACT 1 cut(s) 490
ScrFI CCNGG 3 cut(s) 97, 976, 1402
SduI GDGCHC 5 cut(s) 837, 1126, 1133, 1423, 1585
SfaNI GCATC 4 cut(s) 251, 421, 543, 1492
SfcI CTRYAG 2 cut(s) 525, 1143
SfuI TTCGAA 1 cut(s) 841
SinI GGWCC 1 cut(s) 357
SmiMI CAYNNNNRTG 2 cut(s) 945, 1395
SmlI CTYRAG 2 cut(s) 107, 998
SmoI CTYRAG 2 cut(s) 107, 998
SpeI ACTAGT 1 cut(s) 1022
Sse9I AATT 8 cut(s) 273, 584, 1214, 1277, 1305, 1442, 1633, 1641
SsiI CCGC 5 cut(s) 360, 627, 957, 1227, 1470
SspI AATATT 1 cut(s) 1450
SspMI CTAG 4 cut(s) 437, 755, 1023, 1479
StyD4I CCNGG 3 cut(s) 95, 974, 1400
StyI CCWWGG 1 cut(s) 622
TaaI ACNGT 5 cut(s) 150, 290, 1012, 1135, 1394
TaiI ACGT 2 cut(s) 801, 1330
TaqI TCGA 4 cut(s) 841, 860, 1463, 1650
TasI AATT 8 cut(s) 273, 584, 1214, 1277, 1305, 1442, 1633, 1641
TatI WGTACW 3 cut(s) 111, 401, 488
TauI GCSGC 1 cut(s) 1473
TfiI GAWTC 6 cut(s) 35, 79, 937, 1347, 1357, 1603
Tru1I TTAA 6 cut(s) 276, 315, 588, 1317, 1386, 1440
Tru9I TTAA 6 cut(s) 276, 315, 588, 1317, 1386, 1440
TscAI CASTG 5 cut(s) 555, 1015, 1378, 1418, 1539
TseFI GTSAC 2 cut(s) 284, 799
TseI GCWGC 3 cut(s) 362, 440, 686
Tsp45I GTSAC 2 cut(s) 284, 799
TspDTI ATGAA 7 cut(s) 48, 628, 929, 1005, 1276, 1356, 1380
TspRI CASTG 5 cut(s) 555, 1015, 1378, 1418, 1539
VneI GTGCAC 1 cut(s) 1129
VpaK11BI GGWCC 1 cut(s) 357
VspI ATTAAT 1 cut(s) 1386
XagI CCTNNNNNAGG 1 cut(s) 1265
XapI RAATTY 2 cut(s) 584, 1214
XceI RCATGY 4 cut(s) 823, 1057, 1195, 1562
XmiI GTMKAC 1 cut(s) 600
XmnI GAANNNNTTC 1 cut(s) 83
XspI CTAG 4 cut(s) 437, 755, 1023, 1479
ZrmI AGTACT 1 cut(s) 490
Zsp2I ATGCAT 1 cut(s) 76
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.