Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000004

Orthogroup ID

OG0012440

Physical Location & Seq

Genomic Coordinates Reverse (-)

39483200 .. 39488331

Gene Structure 5132 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug04G0230400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4104 bp

ATGTTTGTACTCTCTCTCCTTACTTCGGCTGCCGGAGGAGTAGTGAGTAAGGTGGCTTCACTTGCTACTGATCAGTTGAGACTTGATATCGTCTGGGGGTTCCGCAAGGAACTGAATAAGCTCAAAGAATCCTCAACTCAGATGGGATATATCATACATGATGCTGCGCACGAAGCTGGAGATCGGGAACTGCGGGCAAGGGCTGAGTGGATGAAGAGGCTTATAATTCATCGGATCGATATAAAGGAGACAAGGCTGGATAAGAAGATACTTGGCTTCTTCTCCAACAATCCTGTTGTATTTCGTCTTCAGATGGCACGCAAGATTTACAACATCAACACATCCTTGGATGATCTCAACAAGCAAGCACCCGGTGTGGGACTCGTCAGATCAAATGGAGGTGCAGCAGCCAGTCAAGGTATGCAGGACAGGGAAACAACCTCATTCTTTGAGAAAAATGAATTCACCTTGAAAGATGCAATCATTGTTGGAAGGGATGAGGTGGTGTCAAATATAATTGCAACCTTGACCAACTCCAACAATCAAGAAAAGATTCTTTCGGTGATGGCCATTGTGGGATTGGGAGGCCTAGGTAAAACAACTTTGGCTCGCTCAGTTACCAAGCAACTGAAAGAAGGTGAAATGGAAAAGTACTTTGATATAACATTCTGGGTGTATGTATCTAATTCTTTTGATGTCAATTCAATTTTACGTCGAATGCTGGAATCATATGACGATGCGACTGGAGCAAATCTTTCAAGTCGGGAAGCATTGATTGGAAGGCTCCAGCTGAAGTTGAAAGAGAAAAGGTATTTTCTTGTACTTGATGACGTTTGGAATGAGGATGCTAATAATTGGGATGATTTGAAGCGTTGTCTGTTGCAACTATGTGCTGCCAAAGGAAGCTCTGTGATTGTCACCACCCGTAGCGCCAAGGTTGCATCAATCATGGAAACACTTCGTAGGTGCGATCTAGAGACTTTATCGGATGATGAATGTTGGTCCATATTGAAGGATAGAGCGTTTGCAGATCCTAATGCTCCAATAGCTTCAGAACTAGAGGGACTCGGACGGAACATTGCCAAAAAGTGCGCTGGTCTTCCATTGGCAACAAAGGTTTTGGGAAGCTTGATGCGTTCTAAGAATAGTAGCAACGAATGGTCGTCAATTCTGGAAAGTAAAATATGGCAGTTATCATCAAACAGAGGGGATAACAGGACCATGCCGGTTCTGAGGTTGAGTTTTGACAATTTGAAATCACCATTGAAACAATGTTTTGCATATTGCTCCATGCTCGAGAGAGGTTCCTCAATTGAAAGAGATAACTTGATCCAACTATGGATGGCTCAAGGTTTTCTCCATCATTCTCATGATCACCAAAGCAAGCAAGGGATTGAAACAGAGGATATAGGCAATAAGTATTTTGATACGCTACTGGAGAACTCTTTGTTTCAAGATGCTACAAAGGATGAGGATGGTGTTATCACCCGATGCAAGATGCATGATCTTGTGCATGATGTTGCAATAGAAGTATCCAAGTGTGAGAGCTTTACACCAGACTTCAATCATGAGATGGATGCTCATGAGATTCGACATGTTGCATGGATTCCTACTCCAATACTAGAAAGGTTGCCAGAAACAAGAATTTCAAAACTGCGCTCACTCTTTTCAAATGTCCAGGTCTCTAATACCATTTTCCCAAAGTTTAAAGCTTTACGTGTCTTAAGTTTTTGTGAGACTGAGAATCAGAAGTTGTTAGACCAAAAAGCAGAGAATCAGGAATTGCCAAATTCACTTGGTAAGCTGAAGCACTTGAGGTATCTAGATATTTCCGATACAGAAATTGAAGCACTCCCAAAGTCTATTGGCAAGCTCTATAACCTCCAAACATTGAAATTACCGGATCTCGAAAAGTGTCCAAAGGAGATTCAAAATTTGATCAACTTGAGGCATTTTTATTTTGGTGAGGGGATGAAATTTGAGGCTGAGATTTTGAGGCGATTAACTAATCTCCGAACACTGCCTTCCTTTAATGTGGGTAAGGAGAGAGGTCCTGCAATTAAGGAGTTGGGTGGGTTGAACCAATTGAGAGGCCACTTAATTATTGATGGGCTACAACATGTAAGGGATGGAGAAGAAGCAAAGAATGCTAGGTTAGTGCAGAAGAATCACTTACGTGAGCTAACATTTAAGTGGACAGACGATGGCTTGGCGGATGTACTAGATGATGGTTCATTAGGTAGTCACGTGCCCCCTAATGAAAATCAGACGGATGTACTAGATGATGCTAGTTCATTACTACCTAATGAAAATCAGACTTATGTACTAGATGGCTTGGAACCTCATGGTAACTTGCAAAGCTTGAAGATTGAAAATTTCATGGGTGCGAGATTTCCGTCATGGATAACGAGTCTCCAAAATTTGAAGCAGATTCGACTACTCAATTGTTGCAGTCGTGAAGGAGTCCCAACACTCGGGCATCTACCTAATCTAAGATCTCTTGAGATTAAGAGGATGAAAAAGCTGAAATGTGTGGGAGCTGAGTTTTATGGTACTAGTACAACTTTGTTTCCAGCACTGAAAGCATTATACATCCTTGGGTGCCAAGAGCTCATTGAATGGATGGAAGCACCGAGGATATCAGGAGAAGTAGTGGTATTTCCTTGCCTCGAAGAGTTGGAACTCAGGTACTGTCCCAAATTGAGAAATGCTCCGAGTCGTTTTCCACGTCTCAAGAGGTTGTGGATAGACGAAATGGACAACAACATGCCAATAGAAAATATAAGCACTCAGCTTACCACTCTCACCAATCTCTATATAGATCATGTAAAGGGACTAACTTGTCTACCAGAAGGGATGCTGAACAATAACAAGTGTCTCACATATTTGCAGATAGTTAATTGTGATGAGTTGACTTGTATTGCTCTCGATGTATCTGACACGCTCCCTTGTCTTGAGGAGTTGAAAATAACTCGTTGTCACAGCCTAGAGTCAATTCGGATCGCACAGGGCATTGCATCTCTCCGTGAATTGTACATCTGGAGGTGTACGGGATTATCAAGCCTAGAGGTTGGGCTCGATTACTGCACCTCTCTTCAGGCGTTGCATATATATTATTGCCCTAATCTGAAGATAATTCCAATCACACAGGGCACGCCGTCTCTCCGGATATTGAGGATTCAAGGATGTGCGGGATTGTCAAGCCTAGGAAGTGGGCTCAACTACTGCACCTCTCTTCAGGAATTGGAGATTAAAGGTTGTCACAATCTAACATCAATTCCAATCACACAGGGCATCACATCTCTCCAGAGGTTGAGGATTATTCGTTGTCAGAGATTATTAAGCCTACCGAGTGGGATCCAATTCTGCACCTCTCTTCAGCATCTGCGTATAACTGGTTGCTCTAGTCTAGTATCCATTTCAGTATCATATGTGTGCGACCACCCGGACTCAACGAATGAACCGGAGGAAGTCTCTACGGGTGTTGTTGAGTATTTGTCTATACAGGAGGATTGTTGTAATCTAGAATCTATTCCACCTAATCTCCGTCAGTTGTTGATATGTCATTGTGGGAAATTCAAATATGTGCCCATAGGATTTCACCGCCTCACTCGTTTGAAGATGCTGCAAATTGGTTTGTTCTGGGAGGAGCTGGACGCCTTCCCTGATTTTCAGCTTCCACCACCATCACATGATTCACAACTTGAAAGCTTAGAGTTGGTGGGGTGGCCTAAGCTCAAGTCTCTGCCCCAACAAATTCAGCACTTTACTTTTTTAAAAACTCTCTGGATAGGAAAGTTTATAGGACTAGAGGCTCTTCCTGAATGGTTGGGAAATCTTACATCTCTTGAGACTCTACATATCTGGCTTTGCAAGAATCTCAAGTATCTACCTACACTAAATGCTATGAAAGGCCTCACCAAATTGAAGAAGCTCTACATTGAGGGATGTCCCCTGCTCAAGGAAAGATGCACCAAGGAGACTGGCCCAGAATGGCTCAAGATTTCTCACATCCCAGAACTTGACTGTGTTAATTCAGCAGCAGCTATGAGAATGAGAAGTATCCACCTCAAGTGGCAGCTATGCAAACAAAAGATGCACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004843 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006464 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009987 GO:0016579 GO:0016787 GO:0019538 GO:0019783 GO:0019784 GO:0032182 GO:0036211 GO:0036459 GO:0043130 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0070011 GO:0070646 GO:0070647 GO:0071108 GO:0071704 GO:0101005 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_C65

Protein Analysis

1367

Amino Acids

154.74

Weight (kDa)

7.16

Isoelectric Point (pI)

43.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NB-ARC	PF00931	167 - 344	8.8e-34	NB-ARC domain
AAA_22	PF13401	188 - 291	9.3e-07	AAA domain
NACHT	PF05729	188 - 330	1.9e-07	NACHT domain
WHD_DRP	PF23559	434 - 508	2.7e-21	Disease resistance protein Winged helix domain
LRR_14	PF23598	552 - 896	1.3e-31	Leucine-rich repeat region
LRR_R13L1-DRL21	PF25019	687 - 839	1.7e-32	R13L1/DRL21 LRRs
LRR_At1g61320_AtMIF1	PF23622	1069 - 1219	4.9e-06	At1g61320/AtMIF1, LRR domain
LRR_14	PF23598	1235 - 1351	4.3e-07	Leucine-rich repeat region
LRR_R13L1-DRL21	PF25019	1256 - 1317	4e-06	R13L1/DRL21 LRRs

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012440)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G28120
fragaria_vesca	FvH4_4g22670 FvH4_4g22670
malus_domestica	MD00G1177400.v1.1 MD13G1130400.v1.1
prunus_persica	Prupe.1G215300_v2.0.a1
pyrus_communis	pycom13g11370
rosa_chinensis	RchiOBHm_Chr4g0428911 RchiOBHm_Chr7g0205601
rosa_laevigata	RLG00000007115
rosa_multiflora	Rmu_sc0008612.1_g000010 Rmu_sc0015419.1_g000004 Rmu_sc0019159.1_g000001
rosa_roxburghii	Rroxscaffold_5G00370580
rosa_rugosa	Rorug04G0230400
rosa_samantha	Rh4BG292700 Rh4CG308800 Rh4DG289900
rosa_wichuraiana	Rw4G024880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	224
AasI	GACNNNNNNGTC	1 cut(s)	2841
Acc16I	TGCGCA	1 cut(s)	168
AccB1I	GGYRCC	1 cut(s)	2601
AccI	GTMKAC	1 cut(s)	2845
AccIII	TCCGGA	1 cut(s)	3165
AciI	CCGC	5 cut(s)	103, 193, 2213, 3191, 3604
AclWI	GGATC	7 cut(s)	242, 1025, 1324, 1911, 3008, 3352, 3365
AcoI	YGGCCR	1 cut(s)	567
AcsI	RAATTY	9 cut(s)	461, 1644, 1789, 1933, 1976, 2374, 2419, 3575, 3756
AcuI	CTGAAG	8 cut(s)	293, 812, 1035, 1826, 3080, 3149, 3221, 3362
AcvI	CACGTG	1 cut(s)	2248
AcyI	GRCGYC	1 cut(s)	3657
AdeI	CACNNNGTG	1 cut(s)	374
AfiI	CCNNNNNNNGG	4 cut(s)	25, 377, 2474, 3961
AflII	CTTAAG	1 cut(s)	1723
AflIII	ACRYGT	3 cut(s)	1594, 1717, 2119
AhlI	ACTAGT	1 cut(s)	2555
AjiI	CACGTC	1 cut(s)	2729
AjnI	CCWGG	1 cut(s)	1677
AjuI	GAANNNNNNNTTGG	2 cut(s)	759, 791
AleI	CACNNNNGTG	1 cut(s)	2175
Alw21I	GWGCWC	1 cut(s)	2613
AlwI	GGATC	7 cut(s)	242, 1025, 1324, 1911, 3008, 3352, 3365
AlwNI	CAGNNNCTG	2 cut(s)	2691, 3385
Ama87I	CYCGRG	2 cut(s)	1295, 2474
Aor13HI	TCCGGA	1 cut(s)	3165
AoxI	GGCC	6 cut(s)	567, 586, 2092, 3728, 3913, 3985
ApeKI	GCWGC	9 cut(s)	29, 164, 404, 407, 893, 3625, 4040, 4043, 4078
ApoI	RAATTY	9 cut(s)	461, 1644, 1789, 1933, 1976, 2374, 2419, 3575, 3756
Asp700I	GAANNNNTTC	3 cut(s)	552, 957, 3531
AspA2I	CCTAGG	2 cut(s)	589, 3205
AspLEI	GCGC	4 cut(s)	169, 932, 1094, 1659
AspS9I	GGNCC	4 cut(s)	1002, 1218, 2051, 3986
AsuC2I	CCSGG	2 cut(s)	372, 3446
AvaI	CYCGRG	2 cut(s)	1295, 2474
AvaII	GGWCC	3 cut(s)	1002, 1218, 2051
AvrII	CCTAGG	2 cut(s)	589, 3205
BaeGI	GKGCMC	3 cut(s)	2253, 3155, 3591
BalI	TGGCCA	1 cut(s)	569
BamHI	GGATCC	1 cut(s)	3357
BanI	GGYRCC	1 cut(s)	2601
BanII	GRGCYC	3 cut(s)	2613, 3078, 3219
BarI	GAAGNNNNNNTAC	6 cut(s)	40, 72, 291, 323, 3920, 3952
BbrPI	CACGTG	1 cut(s)	2248
BbsI	GAAGAC	2 cut(s)	299, 1091
Bbv12I	GWGCWC	1 cut(s)	2613
BbvI	GCAGC	9 cut(s)	16, 151, 416, 419, 880, 3612, 4052, 4055, 4090
BceAI	ACGGC	1 cut(s)	3142
BciT130I	CCWGG	1 cut(s)	1679
BciVI	GTATCC	3 cut(s)	1543, 3424, 4073
BclI	TGATCA	3 cut(s)	70, 1372, 1938
BcnI	CCSGG	2 cut(s)	372, 3446
BcuI	ACTAGT	1 cut(s)	2555
BfoI	RGCGCY	1 cut(s)	933
BfrI	CTTAAG	1 cut(s)	1723
BfuI	GTATCC	3 cut(s)	1543, 3424, 4073
BglII	AGATCT	1 cut(s)	2495
BisI	GCNGC	9 cut(s)	30, 165, 405, 408, 894, 3626, 4041, 4044, 4079
BlnI	CCTAGG	2 cut(s)	589, 3205
BlsI	GCNGC	9 cut(s)	31, 166, 406, 409, 895, 3627, 4042, 4045, 4080
BmcAI	AGTACT	1 cut(s)	653
Bme1390I	CCNGG	3 cut(s)	372, 1679, 3446
Bme18I	GGWCC	3 cut(s)	1002, 1218, 2051
BmeT110I	CYCGRG	2 cut(s)	1295, 2474
BmgBI	CACGTC	1 cut(s)	2729
BmgT120I	GGNCC	4 cut(s)	1002, 1218, 2051, 3986
BmiI	GGNNCC	6 cut(s)	101, 785, 1306, 2340, 2603, 3359
BmrFI	CCNGG	3 cut(s)	372, 1679, 3446
BpiI	GAAGAC	2 cut(s)	299, 1091
BplI	GAGNNNNNCTC	4 cut(s)	2695, 2727, 3458, 3490
BpmI	CTGGAG	6 cut(s)	198, 765, 770, 1457, 3061, 3290
Bpu10I	CCTNAGC	1 cut(s)	3732
BpuMI	CCSGG	2 cut(s)	372, 3446
Bsa29I	ATCGAT	1 cut(s)	237
BsaAI	YACGTR	3 cut(s)	1718, 2177, 2248
BsaHI	GRCGYC	1 cut(s)	3657
BsaI	GGTCTC	1 cut(s)	1687
BsaJI	CCNNGG	7 cut(s)	345, 589, 933, 2596, 2633, 3205, 3975
BsaWI	WCCGGW	3 cut(s)	1900, 3165, 3463
BsaXI	ACNNNNNCTCC	7 cut(s)	27, 30, 57, 2637, 2667, 3528, 3558
Bsc4I	CCNNNNNNNGG	4 cut(s)	25, 377, 2474, 3961
Bse118I	RCCGGY	1 cut(s)	1225
Bse1I	ACTGG	5 cut(s)	411, 748, 1440, 3400, 3988
Bse3DI	GCAATG	2 cut(s)	1077, 3012
BseAI	TCCGGA	1 cut(s)	3165
BseBI	CCWGG	1 cut(s)	1679
BseCI	ATCGAT	1 cut(s)	237
BseDI	CCNNGG	7 cut(s)	345, 589, 933, 2596, 2633, 3205, 3975
BseLI	CCNNNNNNNGG	4 cut(s)	25, 377, 2474, 3961
BseMI	GCAATG	2 cut(s)	1077, 3012
BseMII	CTCAG	9 cut(s)	152, 195, 627, 1223, 1731, 1977, 2532, 2698, 2804
BseNI	ACTGG	5 cut(s)	411, 748, 1440, 3400, 3988
BseRI	GAGGAG	3 cut(s)	51, 2972, 3662
BseSI	GKGCMC	3 cut(s)	2253, 3155, 3591
BseXI	GCAGC	9 cut(s)	16, 151, 416, 419, 880, 3612, 4052, 4055, 4090
BsgI	GTGCAG	5 cut(s)	423, 2180, 3070, 3211, 3352
BshFI	GGCC	6 cut(s)	569, 588, 2094, 3730, 3915, 3987
BshNI	GGYRCC	1 cut(s)	2601
BshVI	ATCGAT	1 cut(s)	237
BsiHKAI	GWGCWC	1 cut(s)	2613
BsiHKCI	CYCGRG	2 cut(s)	1295, 2474
BsiSI	CCGG	7 cut(s)	33, 372, 1226, 1901, 3166, 3446, 3464
BslFI	GGGAC	6 cut(s)	393, 1077, 2450, 2679, 2847, 3936
BslI	CCNNNNNNNGG	4 cut(s)	25, 377, 2474, 3961
BsmBI	CGTCTC	2 cut(s)	2735, 3165
BsmFI	GGGAC	6 cut(s)	393, 1077, 2450, 2679, 2847, 3936
BsmI	GAATGC	2 cut(s)	723, 2152
BsnI	GGCC	6 cut(s)	569, 588, 2094, 3730, 3915, 3987
Bso31I	GGTCTC	1 cut(s)	1687
BsoBI	CYCGRG	2 cut(s)	1295, 2474
Bsp1286I	GDGCHC	6 cut(s)	2253, 2613, 3078, 3155, 3219, 3591
Bsp13I	TCCGGA	1 cut(s)	3165
Bsp1407I	TGTACA	1 cut(s)	3033
BspACI	CCGC	5 cut(s)	103, 193, 2213, 3191, 3604
BspANI	GGCC	6 cut(s)	569, 588, 2094, 3730, 3915, 3987
BspCNI	CTCAG	9 cut(s)	151, 196, 626, 1224, 1732, 1978, 2533, 2697, 2803
BspDI	ATCGAT	1 cut(s)	237
BspEI	TCCGGA	1 cut(s)	3165
BspHI	TCATGA	3 cut(s)	1369, 1567, 1582
BspLI	GGNNCC	6 cut(s)	101, 785, 1306, 2340, 2603, 3359
BspPI	GGATC	7 cut(s)	242, 1025, 1324, 1911, 3008, 3352, 3365
BspQI	GCTCTTC	1 cut(s)	3822
BspT107I	GGYRCC	1 cut(s)	2601
BspTI	CTTAAG	1 cut(s)	1723
BspTNI	GGTCTC	1 cut(s)	1687
BsrDI	GCAATG	2 cut(s)	1077, 3012
BsrFI	RCCGGY	1 cut(s)	1225
BsrGI	TGTACA	1 cut(s)	3033
BsrI	ACTGG	5 cut(s)	411, 748, 1440, 3400, 3988
BssAI	RCCGGY	1 cut(s)	1225
BssECI	CCNNGG	7 cut(s)	345, 589, 933, 2596, 2633, 3205, 3975
BssNI	GRCGYC	1 cut(s)	3657
BssT1I	CCWWGG	6 cut(s)	345, 589, 933, 2596, 3205, 3975
Bst2UI	CCWGG	1 cut(s)	1679
Bst4CI	ACNGT	2 cut(s)	2693, 4028
Bst6I	CTCTTC	6 cut(s)	209, 2667, 3099, 3240, 3381, 3822
BstACI	GRCGYC	1 cut(s)	3657
BstAFI	CTTAAG	1 cut(s)	1723
BstAPI	GCANNNNNTGC	1 cut(s)	2147
BstAUI	TGTACA	1 cut(s)	3033
BstBAI	YACGTR	3 cut(s)	1718, 2177, 2248
BstC8I	GCNNGC	7 cut(s)	195, 319, 366, 610, 1385, 1871, 3155
BstENI	CCTNNNNNAGG	1 cut(s)	3959
BstH2I	RGCGCY	1 cut(s)	933
BstHHI	GCGC	4 cut(s)	169, 932, 1094, 1659
BstMWI	GCNNNNNNNGC	7 cut(s)	62, 173, 746, 938, 1046, 2147, 2582
BstNI	CCWGG	1 cut(s)	1679
BstNSI	RCATGY	3 cut(s)	1598, 2123, 2770
BstSCI	CCNGG	3 cut(s)	370, 1677, 3444
BstSLI	GKGCMC	3 cut(s)	2253, 3155, 3591
BstV1I	GCAGC	9 cut(s)	16, 151, 416, 419, 880, 3612, 4052, 4055, 4090
BstV2I	GAAGAC	2 cut(s)	299, 1091
BstX2I	RGATCY	4 cut(s)	1030, 1903, 2495, 3357
BstYI	RGATCY	4 cut(s)	1030, 1903, 2495, 3357
Bsu15I	ATCGAT	1 cut(s)	237
BsuI	GTATCC	3 cut(s)	1543, 3424, 4073
BsuRI	GGCC	6 cut(s)	569, 588, 2094, 3730, 3915, 3987
BsuTUI	ATCGAT	1 cut(s)	237
BtrI	CACGTC	1 cut(s)	2729
BtsI	GCAGTG	1 cut(s)	2018
BtsIMutI	CAGTG	3 cut(s)	2018, 2576, 4099
Cac8I	GCNNGC	7 cut(s)	195, 319, 366, 610, 1385, 1871, 3155
CaiI	CAGNNNCTG	2 cut(s)	2691, 3385
CciI	TCATGA	3 cut(s)	1369, 1567, 1582
CfoI	GCGC	4 cut(s)	169, 932, 1094, 1659
Cfr10I	RCCGGY	1 cut(s)	1225
Cfr13I	GGNCC	4 cut(s)	1002, 1218, 2051, 3986
ClaI	ATCGAT	1 cut(s)	237
CseI	GACGC	1 cut(s)	3665
DraI	TTTAAA	2 cut(s)	1708, 3777
DraIII	CACNNNGTG	1 cut(s)	374
DrdI	GACNNNNNNGTC	1 cut(s)	2841
DseDI	GACNNNNNNGTC	1 cut(s)	2841
EaeI	YGGCCR	1 cut(s)	567
Eam1104I	CTCTTC	6 cut(s)	209, 2667, 3099, 3240, 3381, 3822
EarI	CTCTTC	6 cut(s)	209, 2667, 3099, 3240, 3381, 3822
EciI	GGCGGA	1 cut(s)	2228
Ecl136II	GAGCTC	1 cut(s)	2611
Eco130I	CCWWGG	6 cut(s)	345, 589, 933, 2596, 3205, 3975
Eco147I	AGGCCT	2 cut(s)	588, 3915
Eco24I	GRGCYC	3 cut(s)	2613, 3078, 3219
Eco31I	GGTCTC	1 cut(s)	1687
Eco32I	GATATC	2 cut(s)	88, 2640
Eco47I	GGWCC	3 cut(s)	1002, 1218, 2051
Eco53kI	GAGCTC	1 cut(s)	2611
Eco57I	CTGAAG	8 cut(s)	293, 812, 1035, 1826, 3080, 3149, 3221, 3362
Eco72I	CACGTG	1 cut(s)	2248
Eco88I	CYCGRG	2 cut(s)	1295, 2474
EcoICRI	GAGCTC	1 cut(s)	2611
EcoNI	CCTNNNNNAGG	1 cut(s)	3959
EcoO109I	RGGNCCY	1 cut(s)	2051
EcoRI	GAATTC	1 cut(s)	461
EcoRII	CCWGG	1 cut(s)	1677
EcoRV	GATATC	2 cut(s)	88, 2640
EcoT14I	CCWWGG	6 cut(s)	345, 589, 933, 2596, 3205, 3975
EcoT22I	ATGCAT	1 cut(s)	1503
EcoT38I	GRGCYC	3 cut(s)	2613, 3078, 3219
ErhI	CCWWGG	6 cut(s)	345, 589, 933, 2596, 3205, 3975
Esp3I	CGTCTC	2 cut(s)	2735, 3165
FalI	AAGNNNNNCTT	2 cut(s)	2156, 2188
FaqI	GGGAC	6 cut(s)	393, 1077, 2450, 2679, 2847, 3936
FauI	CCCGC	2 cut(s)	186, 3184
FauNDI	CATATG	2 cut(s)	730, 3430
FbaI	TGATCA	3 cut(s)	70, 1372, 1938
FblI	GTMKAC	1 cut(s)	2845
Fnu4HI	GCNGC	9 cut(s)	30, 165, 405, 408, 894, 3626, 4041, 4044, 4079
FriOI	GRGCYC	3 cut(s)	2613, 3078, 3219
Fsp4HI	GCNGC	9 cut(s)	30, 165, 405, 408, 894, 3626, 4041, 4044, 4079
FspI	TGCGCA	1 cut(s)	168
GlaI	GCGC	4 cut(s)	168, 931, 1093, 1658
GluI	GCNGC	9 cut(s)	30, 165, 405, 408, 894, 3626, 4041, 4044, 4079
GsuI	CTGGAG	6 cut(s)	198, 765, 770, 1457, 3061, 3290
HaeII	RGCGCY	1 cut(s)	933
HaeIII	GGCC	6 cut(s)	569, 588, 2094, 3730, 3915, 3987
HapII	CCGG	7 cut(s)	33, 372, 1226, 1901, 3166, 3446, 3464
HgaI	GACGC	1 cut(s)	3665
HhaI	GCGC	4 cut(s)	169, 932, 1094, 1659
Hin1I	GRCGYC	1 cut(s)	3657
Hin6I	GCGC	4 cut(s)	167, 930, 1092, 1657
HinP1I	GCGC	4 cut(s)	167, 930, 1092, 1657
HincII	GTYRAC	1 cut(s)	2913
HindII	GTYRAC	1 cut(s)	2913
HindIII	AAGCTT	4 cut(s)	1126, 1710, 2359, 3709
HpaII	CCGG	7 cut(s)	33, 372, 1226, 1901, 3166, 3446, 3464
Hpy166II	GTNNAC	4 cut(s)	2196, 2846, 2913, 3048
Hpy8I	GTNNAC	4 cut(s)	2196, 2846, 2913, 3048
Hpy99I	CGWCG	1 cut(s)	717
HpyAV	CCTTC	8 cut(s)	486, 629, 774, 1006, 2034, 2453, 2846, 3670
HpyCH4III	ACNGT	2 cut(s)	2693, 4028
HpyCH4IV	ACGT	6 cut(s)	712, 831, 1717, 2176, 2247, 2728
HpyF10VI	GCNNNNNNNGC	7 cut(s)	62, 173, 746, 938, 1046, 2147, 2582
HpySE526I	ACGT	6 cut(s)	712, 831, 1717, 2176, 2247, 2728
Hsp92I	GRCGYC	1 cut(s)	3657
HspAI	GCGC	4 cut(s)	167, 930, 1092, 1657
Kpn2I	TCCGGA	1 cut(s)	3165
Ksp22I	TGATCA	3 cut(s)	70, 1372, 1938
LguI	GCTCTTC	1 cut(s)	3822
LmnI	GCTCC	8 cut(s)	746, 789, 1045, 1292, 2537, 2716, 2949, 3649
Lsp1109I	GCAGC	9 cut(s)	16, 151, 416, 419, 880, 3612, 4052, 4055, 4090
MaeII	ACGT	6 cut(s)	712, 831, 1717, 2176, 2247, 2728
MaeIII	GTNAC	6 cut(s)	616, 916, 2243, 2348, 2978, 3260
MfeI	CAATTG	3 cut(s)	1311, 2084, 2443
MflI	RGATCY	4 cut(s)	1030, 1903, 2495, 3357
MhlI	GDGCHC	6 cut(s)	2253, 2613, 3078, 3155, 3219, 3591
MlsI	TGGCCA	1 cut(s)	569
MluNI	TGGCCA	1 cut(s)	569
MlyI	GAGTC	8 cut(s)	375, 1059, 2419, 2472, 2725, 2999, 3443, 3847
MmeI	TCCRAC	5 cut(s)	309, 469, 561, 1357, 2658
Mox20I	TGGCCA	1 cut(s)	569
Mph1103I	ATGCAT	1 cut(s)	1503
MroI	TCCGGA	1 cut(s)	3165
MroXI	GAANNNNTTC	3 cut(s)	552, 957, 3531
MscI	TGGCCA	1 cut(s)	569
MslI	CAYNNNNRTG	4 cut(s)	1368, 2175, 2191, 3433
Msp20I	TGGCCA	1 cut(s)	569
MspA1I	CMGCKG	1 cut(s)	790
MspCI	CTTAAG	1 cut(s)	1723
MspI	CCGG	7 cut(s)	33, 372, 1226, 1901, 3166, 3446, 3464
MspR9I	CCNGG	3 cut(s)	372, 1679, 3446
MunI	CAATTG	3 cut(s)	1311, 2084, 2443
Mva1269I	GAATGC	2 cut(s)	723, 2152
MvaI	CCWGG	1 cut(s)	1679
MwoI	GCNNNNNNNGC	7 cut(s)	62, 173, 746, 938, 1046, 2147, 2582
NciI	CCSGG	2 cut(s)	372, 3446
NdeI	CATATG	2 cut(s)	730, 3430
NlaIV	GGNNCC	6 cut(s)	101, 785, 1306, 2340, 2603, 3359
NmuCI	GTSAC	4 cut(s)	916, 2243, 2978, 3260
NsbI	TGCGCA	1 cut(s)	168
NsiI	ATGCAT	1 cut(s)	1503
NspI	RCATGY	3 cut(s)	1598, 2123, 2770
OliI	CACNNNNGTG	1 cut(s)	2175
PaeR7I	CTCGAG	1 cut(s)	1295
PagI	TCATGA	3 cut(s)	1369, 1567, 1582
PceI	AGGCCT	2 cut(s)	588, 3915
PciI	ACATGT	2 cut(s)	1594, 2119
PciSI	GCTCTTC	1 cut(s)	3822
PcsI	WCGNNNNNNNCGW	1 cut(s)	2725
PctI	GAATGC	2 cut(s)	723, 2152
PdmI	GAANNNNTTC	3 cut(s)	552, 957, 3531
PkrI	GCNGC	9 cut(s)	31, 166, 406, 409, 895, 3627, 4042, 4045, 4080
PleI	GAGTC	8 cut(s)	375, 1059, 2418, 2471, 2724, 2998, 3443, 3847
PmaCI	CACGTG	1 cut(s)	2248
PmlI	CACGTG	1 cut(s)	2248
PpsI	GAGTC	8 cut(s)	375, 1059, 2418, 2471, 2724, 2998, 3443, 3847
Ppu21I	YACGTR	3 cut(s)	1718, 2177, 2248
PpuMI	RGGWCCY	1 cut(s)	2051
PscI	ACATGT	2 cut(s)	1594, 2119
PsiI	TTATAA	1 cut(s)	224
Psp124BI	GAGCTC	1 cut(s)	2613
Psp5II	RGGWCCY	1 cut(s)	2051
Psp6I	CCWGG	1 cut(s)	1677
PspCI	CACGTG	1 cut(s)	2248
PspGI	CCWGG	1 cut(s)	1677
PspN4I	GGNNCC	6 cut(s)	101, 785, 1306, 2340, 2603, 3359
PspPI	GGNCC	4 cut(s)	1002, 1218, 2051, 3986
PspPPI	RGGWCCY	1 cut(s)	2051
PstNI	CAGNNNCTG	2 cut(s)	2691, 3385
PsuI	RGATCY	4 cut(s)	1030, 1903, 2495, 3357
PvuII	CAGCTG	1 cut(s)	790
RseI	CAYNNNNRTG	4 cut(s)	1368, 2175, 2191, 3433
SacI	GAGCTC	1 cut(s)	2613
SapI	GCTCTTC	1 cut(s)	3822
SatI	GCNGC	9 cut(s)	30, 165, 405, 408, 894, 3626, 4041, 4044, 4079
Sau96I	GGNCC	4 cut(s)	1002, 1218, 2051, 3986
ScaI	AGTACT	1 cut(s)	653
SchI	GAGTC	8 cut(s)	375, 1059, 2419, 2472, 2725, 2999, 3443, 3847
ScrFI	CCNGG	3 cut(s)	372, 1679, 3446
SduI	GDGCHC	6 cut(s)	2253, 2613, 3078, 3155, 3219, 3591
Sfr274I	CTCGAG	1 cut(s)	1295
SinI	GGWCC	3 cut(s)	1002, 1218, 2051
SlaI	CTCGAG	1 cut(s)	1295
SmiMI	CAYNNNNRTG	4 cut(s)	1368, 2175, 2191, 3433
SpeI	ACTAGT	1 cut(s)	2555
SseBI	AGGCCT	2 cut(s)	588, 3915
SsiI	CCGC	5 cut(s)	103, 193, 2213, 3191, 3604
SstI	GAGCTC	1 cut(s)	2613
StuI	AGGCCT	2 cut(s)	588, 3915
StyD4I	CCNGG	3 cut(s)	370, 1677, 3444
StyI	CCWWGG	6 cut(s)	345, 589, 933, 2596, 3205, 3975
TaaI	ACNGT	2 cut(s)	2693, 4028
TaiI	ACGT	6 cut(s)	715, 834, 1720, 2179, 2250, 2731
TaqI	TCGA	9 cut(s)	237, 715, 1296, 1591, 1908, 2434, 2670, 2928, 3078
TatI	WGTACW	8 cut(s)	7, 651, 820, 2218, 2275, 2323, 2558, 3033
TscAI	CASTG	2 cut(s)	2025, 2583
TseFI	GTSAC	4 cut(s)	916, 2243, 2978, 3260
TseI	GCWGC	9 cut(s)	29, 164, 404, 407, 893, 3625, 4040, 4043, 4078
Tsp45I	GTSAC	4 cut(s)	916, 2243, 2978, 3260
TspGWI	ACGGA	5 cut(s)	1087, 2285, 2385, 3014, 3536
TspRI	CASTG	2 cut(s)	2025, 2583
Vha464I	CTTAAG	1 cut(s)	1723
VpaK11BI	GGWCC	3 cut(s)	1002, 1218, 2051
XagI	CCTNNNNNAGG	1 cut(s)	3959
XapI	RAATTY	9 cut(s)	461, 1644, 1789, 1933, 1976, 2374, 2419, 3575, 3756
XbaI	TCTAGA	3 cut(s)	973, 1822, 3523
XceI	RCATGY	3 cut(s)	1598, 2123, 2770
XcmI	CCANNNNNNNNNTGG	1 cut(s)	577
XhoI	CTCGAG	1 cut(s)	1295
XmaJI	CCTAGG	2 cut(s)	589, 3205
XmiI	GTMKAC	1 cut(s)	2845
XmnI	GAANNNNTTC	3 cut(s)	552, 957, 3531
ZrmI	AGTACT	1 cut(s)	653
Zsp2I	ATGCAT	1 cut(s)	1503

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.